col6a6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col6a6
Entrez Gene ID	100487679
Full Name	collagen, type VI, alpha 6
General protein information	Preferred Names collagen, type VI, alpha 6 Names collagen alpha-6(VI) chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031904420.1	XP_031760280.1	collagen alpha-6(VI) chain
XM_018094873.1	XP_017950362.1	collagen alpha-6(VI) chain

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Col6a5	NP_001161395.1
Canis lupus familiaris (dog)	COL6A5	XP_853265.3
Rattus norvegicus (Norway rat)	Col6a5	XP_006243950.1
Homo sapiens (human)	COL6A5	NP_001265227.1
Xenopus tropicalis (tropical clawed frog)	col6a6	XP_002937788.2
Pan troglodytes (chimpanzee)	COL6A5	XP_526306.3
	LOC719331	XP_002808380.1

Gene Ontology

The following col6a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col6a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa45060	XM_018094873.1 Latest version!	Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OXa60808	XM_031904420.1 Latest version!	Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col6a6 ( XM_018094873.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950362.1 Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.

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CloneID	OXa45060
Clone ID Related Accession (Same CDS sequence)	XM_018094873.1
Accession Version	XM_018094873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 27% of CDS bases ##RefSeq-Attributes-END##

ATGAAGATCC TACTCTTCAA GTTTCTTTTG TTCTGCTGGC TTCAGATATC TTTTACTCAG 
AAAGCAGCTG TACCTGAGTA TGCAGATCTT GTCTTTCTGG TTGACAGTTC CAGTAACCTG 
GGAGAGAAGG GCTTCGCCCA AGTGAAAACC TTAATTACCA GAATAATCTC TTCATTGCCT 
GTAGGACCAG ACAAATATCG GGTTGGGCTG GCACAATACA ATGATGATTT AGACATTGGT 
TTTCTGCTAA ACACCTACAA GGGAAAGGGC CCAGTTATAA GTCATATAAA AAATAAACTT 
GCATATAAAG GTGGATCTCT AAAGCTTGGC AATGCACTCA CAAAAGCACA TGAAAAGTTC 
TTTAAAGAAG TCAGTGATAG AGACAAATCA GTGTATCCAC CAGTTCTAGT TGTTGTTGCT 
TCTGGATCGT CTCTGGATGA AGTTACCCAG CCAGCCCTTG AGCTTCAACA GGATGGAGTC 
AGAATAGTTT CAGTAGGGTT ACAAACAGCC CCCTTGTCTC AACTGCAAGC CATAGCTTCA 
TCCACTAGGC ATGTCTTACA GGTATCCAAC ATTCGAGATA TCCCTGCAGT CTCTACAACT 
CTGATAGACA GCATTAAGGA TGTGGTGCAA AGTAAGACTG TTGTACCAAC ACCTTCAGCA 
ACAGATGTTA CAGATATATC AGTTTTCCCT ACAGTTATTG AAGATGTAAA TATCACTGTT 
GATTCAAGAA TATACACTTG TGACCAAGAA TTTACTGCAG ACATAGTATT TATAATAGAT 
ACTTCTCGTC ACAAACCTTC TGAAAGTGAA AACTTAAAGA AATTTCTTTC TGATGTCGTA 
TCAACGCTAC CAATAAGTGA AAACTGTGCC CATGTGCATT TGGTTAAATA TGGCAGCAAA 
GCTGAATTTC TGGCCCCACT AGAGACAGGA GTAAACAAAA GCCGTGTGTT GGAGGCCATA 
AAATCCATAA CTCCAATGAA AGAAGAGGCA GCTAACACTG GCCACGCTCT TAACTTTACA 
AGAACTGAAG TTGTTGCTGA TCCAAAGACA AGTAGGAAGG GACAAGGAAT AAAGCAAATT 
AGTATTTTGA TCACTCATAG TCCATCTGCA GATAATGTCA CTGATGCTGC GCATCTACTG 
CGACAAGACA GAGTAACAAG CTTTGCCTTG GGGATTGCAG AAGCCAAAAA AAATCAAATG 
GACCAAATAG CATCCTACCC ACCTGACAGT TATACTATAA GCTTAAAGGG ATTTTCAGAC 
CTTTCTACAC AGGTGGAATA TTTACAAAAA AATATTCAGA ATGAGCTGGA AGATTACACA 
GTACAATCCT ATGCAAGAAC AGGCCTTATT CAACAAGGAT GCCTGGACAC AGAGGCTGCT 
GATATCTATT TACTTGTTGA TGAGTCTGGC AGCATTACTA CTGACAACTT CCAAGAAATG 
AAGACATTTT TGAAAAAACT TGTAGAAATG TTTGACATAG GACCTGAAAA GGTTCTGGTT 
AGATGTGTAC TCTATTCAGA TTCACCAAGA GAGATTCCAA TCGGTAGAGA CTTTAGTAAA 
GATGAATTAA AATCTGCCCT GCAAAATCTA AGGCAATTAG GAGGTGGAAC AAATACTGGA 
AAAGCTATTA ATTTCACTCA GCAGGTAATT ACAAAAACAG AAAACACTAG GCCTGGGGAT 
GTGCCAGTAT ATCTGATTGT CCTTACAGAT GGAGAGTCCC AGGACAGTGT GAAGGAGGCT 
GCTGAGATTG TCCGGGGTCA TGGTGTAAAC ACTTATGCAA TTGGAGTAAA AGGGTATAAT 
AAAACTCAGT TGCTGGAGAT CACAGGAGCA GAGCACAGGG TTCACTTAGC CCACGACTTT 
GATTCCTTGA AACACATTAA GGATGTTATA GCACAGCAAA TCTGTTTTGG AAAAGCATGT 
CAAGACTTAA TGGCAGACAT TTTGTTTCTG GTTGATAGTT CCAGTGGTAT GGGATCAGAC 
AAGTTTAGCA AAATGAAAAC ATTCATGAAA GATCTGGTCA ACAAAACGGA GGTTGGATTG 
ACAGGTGTAC AGTTTGCAGC TGTTCAGTTC AGTAATTCAA TTAAGGAAGA ATTCCAGTTC 
AATAAACATG CAACCAAAAA TGCCATTTGG GACTCCATAG ACAACATGAA TTTAATGGGC 
AATGTTGCAA AGACTGGAAA TGCCTTAGCA AATGTAGCTG ACTACTTTAC AGAGGCTAAA 
GGAGCAAGAC CAAGTTCAAA AGTTAGTAAA ATTCTTCTAC TGATCACAGA TAATCCATCA 
CAAGATGAAG TAAAAGTTCC AGCCGATTCT TTAAGAAGCA ATGGATTGAT CATCTATTCT 
ATAGGTGGCT TCAGTGCAAA TAAAAAAGAA CTTCAAGAGA TAAGTGGAAA AATAACACCT 
TACTACCAGT CATTTGACAA ACTGCAGACA ATTGAAAATG ATTTGCTTTT TAGAATTTGC 
AATCCTGAAG AAGAATGCAA GAGAATAGAA GTGGCAGATA TTGTCTTTGT AATTGATTCA 
TCTGGCAGTA TAGACTATAC AGAATATAAG GAAATGCAAA ATTTTATGGT ATCGTTGGTG 
AACAAGTCAG CTGTTGGACC AGATAATGTT CAATTCGGCG CTCTCAAATA CTCTGATTAC 
AACACAGAAC TCTTCTACCT GAACAGGTAT ACAAACAAAG TGGACATTAT AAATCATATC 
AACAAAGATA CAACACAAGG TGGAAACACA TACACTGCAG GAGCGGTTAG GTTTTCAAAA 
GAGTTTTTCA CAGAGAAACA TGGAAGCCGC AAGGCAAGGG GAGTGCCTCA GATTGTAATG 
GTTATAACTG ATGGAGATTC TCATGATAAA GATAAACTGA ATGAGACAGC AAGGCAGCTA 
GAACAAGAAG GCATCATCAT CTATGCTATC GGCATTGATC AAGCAAACAC CAATGAGCTG 
GAAACATTGG CTGGGACAGA AGGCAAATGG TTCATGGTAG CTAACTTCAG TGGATTACAG 
GACATCCTTG TACAGGTATC AGAGGCAATG TGCAATAAAA CAGACTGCAA GGTTGAACAA 
GCAGATCTGT TGTTTCTTAT TGATGGATCC AGTAATACTT CTGAAGAAAC ATTCAGGGAA 
ATAAAAAATT TTGTGGTATC AGTTATGGAT GATTTTAATG TTGGCCCTGT AAATGTCCAT 
ATAGCTGTTT CTCAGTACAG TGAGTCTTGC ATTCGAGAAA TTAATTTTGA CTATTCTACT 
GAAAGAGGAA CTTTAAAGAA TGAAATAATA AACATCAGAA AGACCAAAGG GCGGCGTCAT 
ATTGGAGCAG CACTTGATTT CACAAAATCT ACCGTGTATA GCCCATCCAG TGATAACCGT 
TTAAACCAGG GTGTGAAGCA GCTTCTTGTA GTGATTACTG CTGGAAATGC ATCAGATCAA 
GTTGCACGAC CCGCGAAGGC TCTTAGGGAC CGTGGAGTCG ATATATATGC TGTGGGAATA 
GGAAATATTT GCAAGACACA GCTTACACAG ATAACTGGAT CACCTGAGAA GATATATACT 
GATGATGTCA GTGGCTTGAA AGCAATTAAA AAGAGGCTTG TTAGGGATAC ATGTTCAAAA 
GATCCTGTTA CCAATTGTTC CCTTGATGTT GTGGTCAGTT TCGACATCTC CACCCAGTCC 
AGAGGTCAAC GTTTATTCCA TGACCATCCT GAACTAGAAA CTATCCTGCC AAACGTTTTA 
AAGTCAATGG TGTCTCTGAA AAGCCCAAGC TGTGGTGCAG GAAAGCAGCC TCAAGCTTCT 
GTGGCATTTT ATGTGAAAGA TACAAACAGT GTTTCCAGAT TCAACACCTA CAGCCAAGAC 
CTATTTAATA AATTAAAGGA AGTTAAAGTT AGTGGCCCTT CATACCTTGA TTCTTTTTTT 
CTGAAGTCTT CATGGGATAT CTTTAAGAAT ACAGACAAAG GAAAAGCAAA GATGCTACTC 
GTGTTCACTG ATGGACTAGA TGAAGATGTA AAACAACTTG AATACACAGC AGGGTTTATT 
CGATCCCAAG GGCTGAATGC ATTAGTTACA GTTGCTTTGG AAGGAAGCAA AAAGGTGGAG 
GACATGAAGA TGATTGAATT TGGCAGAGGT TTTGAATATA AAAATCAGAT GCATGTTGGA 
ATGCCAGGCA TTGGTCTCAG GTTGGCCAGA CAAGCAGGAA GAAATGGGGC TAAAGGACAG 
CAAGGACACA GAGGAGATGA TGGTGAAAGT GGTGAAAGAG GATTACTTGG TCCAACTGGT 
GAGCAAGGAA TCAAGGGATG CGATGGAGCC AGAGGACGCA AGGGAAACAG AGGATATGCA 
GGAAGCAAAA GTGATGATGG TGAGCCTGGA CTTGAAGGAT TGCCAGGAGA ACAGGGTAGT 
CCTGGTTCAC GAGGAATTAA AGGAGACGAA GGAAATGAAG GCTTTCGAGG AGCTCCAGGT 
CAACCTGGAA CTAGTAGCTC TATCCCTGGT CCCCCCGGTT TGAAAGGAGA TCCAGGACAA 
GAGGGTGAAG CTGGGCGTCA GGGTCCACCG GGACCTCCTG GAAGCAGAGG AACAGGGGGA 
ATAAAGGGAG AGCCTGGTGA GACTGGCTAT GCTGGGGATC AGGGAGAGCG AGGACCTCAG 
GGAGACATTG GGAGCAGTGG ATTAAAAGGT GAAAAAGGAC TTAGTGGATC CCAAGGAAAT 
CGAGGACCAC CTGGTATTGA AGGTTCTACA GGGAGTCCAG GGAAACCTGG ACCATTGGGG 
AGAAAGGGAG AGTCTGAAGG GCCTGGACAA AAAGGAGAAC CTGGAACAGC TGGAAAAAGA 
GGACAGCCGG GTGAAGATGG AAGATCTGGT TATGGTAAAG CAGGAAAAAA AGGAGTGAAG 
GGACAACAAG GATTTCCAGG GGAAATAGGT GTTATGGGAC CAAGAGGAGA TGCTGGGATG 
GATGGAGTAA AAGGTCTAAA GGGATACAAA GGGAACCCGG GTCCTTCATC TAAAGGGGAA 
GCTGGAAATC GAGGAATTCC CGGGTACCCA GGCCGGATGG GAAGAAAAGG AGACCGTGGG 
CCCTCACCAC CCTCTGCATG TGAACTAATT GAATTTGTAC GTGATAAATG CAGAAAACCT 
CCCTGCCCTG TGTATCCTAC AGAATTAGTT TTTGCTCTAG AGATGTCAAG TGCAGTGACC 
CCAGCAATTT ACACACGAAT GCTAACACTG ACAAAAGATA TTGTGAGCAA CATCACAATC 
AGAGCAAGTA ACTGTCCAGC AGGGGCTCGC GTGGCAGTTG TGTCCTATAA TTCAAATATA 
AAGCACCTCA TACGATTCTC TGACTTTCAA ATCAAGGACA AGTTGATAAA GGCTGTGGAA 
AGTATTCCTC TGGAAAAGTC CTCTCAAAGC CCTGATGTTG GGGGTGCCAT GACGTTTGTA 
GCAAGAAATA TTTTTAAGAG ATCCCTTCAA GGCTTCACAG TCAGAAGAAT CGCTGTGCTT 
CTCAGCAGTG GACGTTCTGA AAATGCCAAT AACATCAACA TGGCAGTCAT GGAATATGGT 
GCTCTTGGTA TCATTCCAGT GGTATTTGCA TTCAACCCAG CACCTCTTAT CAAGCGTGCC 
TTTGAAATGG ATGCAACAGG AGCTTTTGAA CTGATTGAAA TGAAACCAAA TGTAGATCCA 
TTAAAAAATT TCAAGCTGTG CACTCTGTGT TTTGACAAAT GTCGGCCAGA AAAGTCCTGT 
GGCGACAAAT CAACTCAACA AAAGCCACCT ATGGATGTTG CCTTTCTTCT TGATAGTTCA 
TACAATGTGA AATACGATGA ATTTGAAGCA GCAAGAGAAT TTTTAGGCAG CATGATTGAT 
CATTTTGACA TTGAAGGATC AGCTTCTGAT AGAGTAGCAC TTGTGACCTT TAATGAAAGT 
TATCATGTTG ACTTTAACTT ATTATCCTAT GACAACAAAA GTAGGATCAA GAAACACATA 
CAGGAAAGTG TTCAACAGGT CAGTGGCCAA CCAGCTCTTG GCTTATCGCT ACAATATACA 
ATTGAGAACA TTATGGCAAA GACACCCAAT CTAAGGAAAC ACAAAGTCAT AATTATTATA 
TTATTTGGAG AGACAAGCCA GTGGGACAAG CAGACACTCA GTGATGCCTC TCAAAAAGCC 
AAATGTGAAG GCTATGCTGT ATTTGTGCTT TCCATTGGTA GATCACATAA CAACACTGAA 
CTGACAGAGC TTGCAAGTAA ACCTTTGGAT CATCACTTAC TTGGACTTGG TAGAATACAC 
AAACCAGACC TTGTGTATGC CAGTGGGTTT CTGCAACAAT TTCTTAATTA TGTGAGAAGA 
GGTATTGGCA AGTATCCCTC AGCCGAGCTC AAGAAGAAAT GCTCAGCAGC TGCAGTGCCA 
GTCAGCAGAA GAAGATTGCC CTCACAGACT GATGCGGAAG AAATGAGAAG AGCAGGAGCT 
GAATATCTTT CTGATTTGCA GTTGGACTTT ATCTCTCAGA AACAAAGCAG CAACATGGAC 
AACTTAGCAT TCCAGAACCT AGAAAGAGAG TTACTCAGGG CTGACTCTGA AGAAGCCAAG 
ACGGGCCACA TCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950362.1
CDS	1..6558
Translation	MKILLFKFLLFCWLQISFTQKAAVPEYADLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALELQQDGVRIVSVGLQTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQSKTVVPTPSATDVTDISVFPTVIEDVNITVDSRIYTCDQEFTADIVFIIDTSRHKPSESENLKKFLSDVVSTLPISENCAHVHLVKYGSKAEFLAPLETGVNKSRVLEAIKSITPMKEEAANTGHALNFTRTEVVADPKTSRKGQGIKQISILITHSPSADNVTDAAHLLRQDRVTSFALGIAEAKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSDSPREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTDGESQDSVKEAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQICFGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVADYFTEAKGARPSSKVSKILLLITDNPSQDEVKVPADSLRSNGLIIYSIGGFSANKKELQEISGKITPYYQSFDKLQTIENDLLFRICNPEEECKRIEVADIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYNTELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDGDSHDKDKLNETARQLEQEGIIIYAIGIDQANTNELETLAGTEGKWFMVANFSGLQDILVQVSEAMCNKTDCKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAGNASDQVARPAKALRDRGVDIYAVGIGNICKTQLTQITGSPEKIYTDDVSGLKAIKKRLVRDTCSKDPVTNCSLDVVVSFDISTQSRGQRLFHDHPELETILPNVLKSMVSLKSPSCGAGKQPQASVAFYVKDTNSVSRFNTYSQDLFNKLKEVKVSGPSYLDSFFLKSSWDIFKNTDKGKAKMLLVFTDGLDEDVKQLEYTAGFIRSQGLNALVTVALEGSKKVEDMKMIEFGRGFEYKNQMHVGMPGIGLRLARQAGRNGAKGQQGHRGDDGESGERGLLGPTGEQGIKGCDGARGRKGNRGYAGSKSDDGEPGLEGLPGEQGSPGSRGIKGDEGNEGFRGAPGQPGTSSSIPGPPGLKGDPGQEGEAGRQGPPGPPGSRGTGGIKGEPGETGYAGDQGERGPQGDIGSSGLKGEKGLSGSQGNRGPPGIEGSTGSPGKPGPLGRKGESEGPGQKGEPGTAGKRGQPGEDGRSGYGKAGKKGVKGQQGFPGEIGVMGPRGDAGMDGVKGLKGYKGNPGPSSKGEAGNRGIPGYPGRMGRKGDRGPSPPSACELIEFVRDKCRKPPCPVYPTELVFALEMSSAVTPAIYTRMLTLTKDIVSNITIRASNCPAGARVAVVSYNSNIKHLIRFSDFQIKDKLIKAVESIPLEKSSQSPDVGGAMTFVARNIFKRSLQGFTVRRIAVLLSSGRSENANNINMAVMEYGALGIIPVVFAFNPAPLIKRAFEMDATGAFELIEMKPNVDPLKNFKLCTLCFDKCRPEKSCGDKSTQQKPPMDVAFLLDSSYNVKYDEFEAAREFLGSMIDHFDIEGSASDRVALVTFNESYHVDFNLLSYDNKSRIKKHIQESVQQVSGQPALGLSLQYTIENIMAKTPNLRKHKVIIIILFGETSQWDKQTLSDASQKAKCEGYAVFVLSIGRSHNNTELTELASKPLDHHLLGLGRIHKPDLVYASGFLQQFLNYVRRGIGKYPSAELKKKCSAAAVPVSRRRLPSQTDAEEMRRAGAEYLSDLQLDFISQKQSSNMDNLAFQNLERELLRADSEEAKTGHIR

Target ORF information:

RefSeq Version	XM_018094873.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094873.1

ORF Insert Sequence:

ATGAAGATCC TACTCTTCAA GTTTCTTTTG TTCTGCTGGC TTCAGATATC TTTTACTCAG 
AAAGCAGCTG TACCTGAGTA TGCAGATCTT GTCTTTCTGG TTGACAGTTC CAGTAACCTG 
GGAGAGAAGG GCTTCGCCCA AGTGAAAACC TTAATTACCA GAATAATCTC TTCATTGCCT 
GTAGGACCAG ACAAATATCG GGTTGGGCTG GCACAATACA ATGATGATTT AGACATTGGT 
TTTCTGCTAA ACACCTACAA GGGAAAGGGC CCAGTTATAA GTCATATAAA AAATAAACTT 
GCATATAAAG GTGGATCTCT AAAGCTTGGC AATGCACTCA CAAAAGCACA TGAAAAGTTC 
TTTAAAGAAG TCAGTGATAG AGACAAATCA GTGTATCCAC CAGTTCTAGT TGTTGTTGCT 
TCTGGATCGT CTCTGGATGA AGTTACCCAG CCAGCCCTTG AGCTTCAACA GGATGGAGTC 
AGAATAGTTT CAGTAGGGTT ACAAACAGCC CCCTTGTCTC AACTGCAAGC CATAGCTTCA 
TCCACTAGGC ATGTCTTACA GGTATCCAAC ATTCGAGATA TCCCTGCAGT CTCTACAACT 
CTGATAGACA GCATTAAGGA TGTGGTGCAA AGTAAGACTG TTGTACCAAC ACCTTCAGCA 
ACAGATGTTA CAGATATATC AGTTTTCCCT ACAGTTATTG AAGATGTAAA TATCACTGTT 
GATTCAAGAA TATACACTTG TGACCAAGAA TTTACTGCAG ACATAGTATT TATAATAGAT 
ACTTCTCGTC ACAAACCTTC TGAAAGTGAA AACTTAAAGA AATTTCTTTC TGATGTCGTA 
TCAACGCTAC CAATAAGTGA AAACTGTGCC CATGTGCATT TGGTTAAATA TGGCAGCAAA 
GCTGAATTTC TGGCCCCACT AGAGACAGGA GTAAACAAAA GCCGTGTGTT GGAGGCCATA 
AAATCCATAA CTCCAATGAA AGAAGAGGCA GCTAACACTG GCCACGCTCT TAACTTTACA 
AGAACTGAAG TTGTTGCTGA TCCAAAGACA AGTAGGAAGG GACAAGGAAT AAAGCAAATT 
AGTATTTTGA TCACTCATAG TCCATCTGCA GATAATGTCA CTGATGCTGC GCATCTACTG 
CGACAAGACA GAGTAACAAG CTTTGCCTTG GGGATTGCAG AAGCCAAAAA AAATCAAATG 
GACCAAATAG CATCCTACCC ACCTGACAGT TATACTATAA GCTTAAAGGG ATTTTCAGAC 
CTTTCTACAC AGGTGGAATA TTTACAAAAA AATATTCAGA ATGAGCTGGA AGATTACACA 
GTACAATCCT ATGCAAGAAC AGGCCTTATT CAACAAGGAT GCCTGGACAC AGAGGCTGCT 
GATATCTATT TACTTGTTGA TGAGTCTGGC AGCATTACTA CTGACAACTT CCAAGAAATG 
AAGACATTTT TGAAAAAACT TGTAGAAATG TTTGACATAG GACCTGAAAA GGTTCTGGTT 
AGATGTGTAC TCTATTCAGA TTCACCAAGA GAGATTCCAA TCGGTAGAGA CTTTAGTAAA 
GATGAATTAA AATCTGCCCT GCAAAATCTA AGGCAATTAG GAGGTGGAAC AAATACTGGA 
AAAGCTATTA ATTTCACTCA GCAGGTAATT ACAAAAACAG AAAACACTAG GCCTGGGGAT 
GTGCCAGTAT ATCTGATTGT CCTTACAGAT GGAGAGTCCC AGGACAGTGT GAAGGAGGCT 
GCTGAGATTG TCCGGGGTCA TGGTGTAAAC ACTTATGCAA TTGGAGTAAA AGGGTATAAT 
AAAACTCAGT TGCTGGAGAT CACAGGAGCA GAGCACAGGG TTCACTTAGC CCACGACTTT 
GATTCCTTGA AACACATTAA GGATGTTATA GCACAGCAAA TCTGTTTTGG AAAAGCATGT 
CAAGACTTAA TGGCAGACAT TTTGTTTCTG GTTGATAGTT CCAGTGGTAT GGGATCAGAC 
AAGTTTAGCA AAATGAAAAC ATTCATGAAA GATCTGGTCA ACAAAACGGA GGTTGGATTG 
ACAGGTGTAC AGTTTGCAGC TGTTCAGTTC AGTAATTCAA TTAAGGAAGA ATTCCAGTTC 
AATAAACATG CAACCAAAAA TGCCATTTGG GACTCCATAG ACAACATGAA TTTAATGGGC 
AATGTTGCAA AGACTGGAAA TGCCTTAGCA AATGTAGCTG ACTACTTTAC AGAGGCTAAA 
GGAGCAAGAC CAAGTTCAAA AGTTAGTAAA ATTCTTCTAC TGATCACAGA TAATCCATCA 
CAAGATGAAG TAAAAGTTCC AGCCGATTCT TTAAGAAGCA ATGGATTGAT CATCTATTCT 
ATAGGTGGCT TCAGTGCAAA TAAAAAAGAA CTTCAAGAGA TAAGTGGAAA AATAACACCT 
TACTACCAGT CATTTGACAA ACTGCAGACA ATTGAAAATG ATTTGCTTTT TAGAATTTGC 
AATCCTGAAG AAGAATGCAA GAGAATAGAA GTGGCAGATA TTGTCTTTGT AATTGATTCA 
TCTGGCAGTA TAGACTATAC AGAATATAAG GAAATGCAAA ATTTTATGGT ATCGTTGGTG 
AACAAGTCAG CTGTTGGACC AGATAATGTT CAATTCGGCG CTCTCAAATA CTCTGATTAC 
AACACAGAAC TCTTCTACCT GAACAGGTAT ACAAACAAAG TGGACATTAT AAATCATATC 
AACAAAGATA CAACACAAGG TGGAAACACA TACACTGCAG GAGCGGTTAG GTTTTCAAAA 
GAGTTTTTCA CAGAGAAACA TGGAAGCCGC AAGGCAAGGG GAGTGCCTCA GATTGTAATG 
GTTATAACTG ATGGAGATTC TCATGATAAA GATAAACTGA ATGAGACAGC AAGGCAGCTA 
GAACAAGAAG GCATCATCAT CTATGCTATC GGCATTGATC AAGCAAACAC CAATGAGCTG 
GAAACATTGG CTGGGACAGA AGGCAAATGG TTCATGGTAG CTAACTTCAG TGGATTACAG 
GACATCCTTG TACAGGTATC AGAGGCAATG TGCAATAAAA CAGACTGCAA GGTTGAACAA 
GCAGATCTGT TGTTTCTTAT TGATGGATCC AGTAATACTT CTGAAGAAAC ATTCAGGGAA 
ATAAAAAATT TTGTGGTATC AGTTATGGAT GATTTTAATG TTGGCCCTGT AAATGTCCAT 
ATAGCTGTTT CTCAGTACAG TGAGTCTTGC ATTCGAGAAA TTAATTTTGA CTATTCTACT 
GAAAGAGGAA CTTTAAAGAA TGAAATAATA AACATCAGAA AGACCAAAGG GCGGCGTCAT 
ATTGGAGCAG CACTTGATTT CACAAAATCT ACCGTGTATA GCCCATCCAG TGATAACCGT 
TTAAACCAGG GTGTGAAGCA GCTTCTTGTA GTGATTACTG CTGGAAATGC ATCAGATCAA 
GTTGCACGAC CCGCGAAGGC TCTTAGGGAC CGTGGAGTCG ATATATATGC TGTGGGAATA 
GGAAATATTT GCAAGACACA GCTTACACAG ATAACTGGAT CACCTGAGAA GATATATACT 
GATGATGTCA GTGGCTTGAA AGCAATTAAA AAGAGGCTTG TTAGGGATAC ATGTTCAAAA 
GATCCTGTTA CCAATTGTTC CCTTGATGTT GTGGTCAGTT TCGACATCTC CACCCAGTCC 
AGAGGTCAAC GTTTATTCCA TGACCATCCT GAACTAGAAA CTATCCTGCC AAACGTTTTA 
AAGTCAATGG TGTCTCTGAA AAGCCCAAGC TGTGGTGCAG GAAAGCAGCC TCAAGCTTCT 
GTGGCATTTT ATGTGAAAGA TACAAACAGT GTTTCCAGAT TCAACACCTA CAGCCAAGAC 
CTATTTAATA AATTAAAGGA AGTTAAAGTT AGTGGCCCTT CATACCTTGA TTCTTTTTTT 
CTGAAGTCTT CATGGGATAT CTTTAAGAAT ACAGACAAAG GAAAAGCAAA GATGCTACTC 
GTGTTCACTG ATGGACTAGA TGAAGATGTA AAACAACTTG AATACACAGC AGGGTTTATT 
CGATCCCAAG GGCTGAATGC ATTAGTTACA GTTGCTTTGG AAGGAAGCAA AAAGGTGGAG 
GACATGAAGA TGATTGAATT TGGCAGAGGT TTTGAATATA AAAATCAGAT GCATGTTGGA 
ATGCCAGGCA TTGGTCTCAG GTTGGCCAGA CAAGCAGGAA GAAATGGGGC TAAAGGACAG 
CAAGGACACA GAGGAGATGA TGGTGAAAGT GGTGAAAGAG GATTACTTGG TCCAACTGGT 
GAGCAAGGAA TCAAGGGATG CGATGGAGCC AGAGGACGCA AGGGAAACAG AGGATATGCA 
GGAAGCAAAA GTGATGATGG TGAGCCTGGA CTTGAAGGAT TGCCAGGAGA ACAGGGTAGT 
CCTGGTTCAC GAGGAATTAA AGGAGACGAA GGAAATGAAG GCTTTCGAGG AGCTCCAGGT 
CAACCTGGAA CTAGTAGCTC TATCCCTGGT CCCCCCGGTT TGAAAGGAGA TCCAGGACAA 
GAGGGTGAAG CTGGGCGTCA GGGTCCACCG GGACCTCCTG GAAGCAGAGG AACAGGGGGA 
ATAAAGGGAG AGCCTGGTGA GACTGGCTAT GCTGGGGATC AGGGAGAGCG AGGACCTCAG 
GGAGACATTG GGAGCAGTGG ATTAAAAGGT GAAAAAGGAC TTAGTGGATC CCAAGGAAAT 
CGAGGACCAC CTGGTATTGA AGGTTCTACA GGGAGTCCAG GGAAACCTGG ACCATTGGGG 
AGAAAGGGAG AGTCTGAAGG GCCTGGACAA AAAGGAGAAC CTGGAACAGC TGGAAAAAGA 
GGACAGCCGG GTGAAGATGG AAGATCTGGT TATGGTAAAG CAGGAAAAAA AGGAGTGAAG 
GGACAACAAG GATTTCCAGG GGAAATAGGT GTTATGGGAC CAAGAGGAGA TGCTGGGATG 
GATGGAGTAA AAGGTCTAAA GGGATACAAA GGGAACCCGG GTCCTTCATC TAAAGGGGAA 
GCTGGAAATC GAGGAATTCC CGGGTACCCA GGCCGGATGG GAAGAAAAGG AGACCGTGGG 
CCCTCACCAC CCTCTGCATG TGAACTAATT GAATTTGTAC GTGATAAATG CAGAAAACCT 
CCCTGCCCTG TGTATCCTAC AGAATTAGTT TTTGCTCTAG AGATGTCAAG TGCAGTGACC 
CCAGCAATTT ACACACGAAT GCTAACACTG ACAAAAGATA TTGTGAGCAA CATCACAATC 
AGAGCAAGTA ACTGTCCAGC AGGGGCTCGC GTGGCAGTTG TGTCCTATAA TTCAAATATA 
AAGCACCTCA TACGATTCTC TGACTTTCAA ATCAAGGACA AGTTGATAAA GGCTGTGGAA 
AGTATTCCTC TGGAAAAGTC CTCTCAAAGC CCTGATGTTG GGGGTGCCAT GACGTTTGTA 
GCAAGAAATA TTTTTAAGAG ATCCCTTCAA GGCTTCACAG TCAGAAGAAT CGCTGTGCTT 
CTCAGCAGTG GACGTTCTGA AAATGCCAAT AACATCAACA TGGCAGTCAT GGAATATGGT 
GCTCTTGGTA TCATTCCAGT GGTATTTGCA TTCAACCCAG CACCTCTTAT CAAGCGTGCC 
TTTGAAATGG ATGCAACAGG AGCTTTTGAA CTGATTGAAA TGAAACCAAA TGTAGATCCA 
TTAAAAAATT TCAAGCTGTG CACTCTGTGT TTTGACAAAT GTCGGCCAGA AAAGTCCTGT 
GGCGACAAAT CAACTCAACA AAAGCCACCT ATGGATGTTG CCTTTCTTCT TGATAGTTCA 
TACAATGTGA AATACGATGA ATTTGAAGCA GCAAGAGAAT TTTTAGGCAG CATGATTGAT 
CATTTTGACA TTGAAGGATC AGCTTCTGAT AGAGTAGCAC TTGTGACCTT TAATGAAAGT 
TATCATGTTG ACTTTAACTT ATTATCCTAT GACAACAAAA GTAGGATCAA GAAACACATA 
CAGGAAAGTG TTCAACAGGT CAGTGGCCAA CCAGCTCTTG GCTTATCGCT ACAATATACA 
ATTGAGAACA TTATGGCAAA GACACCCAAT CTAAGGAAAC ACAAAGTCAT AATTATTATA 
TTATTTGGAG AGACAAGCCA GTGGGACAAG CAGACACTCA GTGATGCCTC TCAAAAAGCC 
AAATGTGAAG GCTATGCTGT ATTTGTGCTT TCCATTGGTA GATCACATAA CAACACTGAA 
CTGACAGAGC TTGCAAGTAA ACCTTTGGAT CATCACTTAC TTGGACTTGG TAGAATACAC 
AAACCAGACC TTGTGTATGC CAGTGGGTTT CTGCAACAAT TTCTTAATTA TGTGAGAAGA 
GGTATTGGCA AGTATCCCTC AGCCGAGCTC AAGAAGAAAT GCTCAGCAGC TGCAGTGCCA 
GTCAGCAGAA GAAGATTGCC CTCACAGACT GATGCGGAAG AAATGAGAAG AGCAGGAGCT 
GAATATCTTT CTGATTTGCA GTTGGACTTT ATCTCTCAGA AACAAAGCAG CAACATGGAC 
AACTTAGCAT TCCAGAACCT AGAAAGAGAG TTACTCAGGG CTGACTCTGA AGAAGCCAAG 
ACGGGCCACA TCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col6a6 ( XM_031904420.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031760280.1 Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.

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CloneID	OXa60808
Clone ID Related Accession (Same CDS sequence)	XM_031904420.1
Accession Version	XM_031904420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	collagen alpha-6(VI) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC TACTCTTCAA GTTTCTTTTG TTCTGCTGGC TTCAGATATC TTTTACTCAG 
AAAGCAGCTG TACCTGAGTA TGCAGATCTT GTCTTTCTGG TTGACAGTTC CAGTAACCTG 
GGAGAGAAGG GCTTCGCCCA AGTGAAAACC TTAATTACCA GAATAATCTC TTCATTGCCT 
GTAGGACCAG ACAAATATCG GGTTGGGCTG GCACAATACA ATGATGATTT AGACATTGGT 
TTTCTGCTAA ACACCTACAA GGGAAAGGGC CCAGTTATAA GTCATATAAA AAATAAACTT 
GCATATAAAG GTGGATCTCT AAAGCTTGGC AATGCACTCA CAAAAGCACA TGAAAAGTTC 
TTTAAAGAAG TCAGTGATAG AGACAAATCA GTGTATCCAC CAGTTCTAGT TGTTGTTGCT 
TCTGGATCGT CTCTGGATGA AGTTACCCAG CCAGCCCTTG AGCTTCAACA GGATGGAGTC 
AGAATAGTTT CAGTAGGGTT ACAAACAGCC CCCTTGTCTC AACTGCAAGC CATAGCTTCA 
TCCACTAGGC ATGTCTTACA GGTATCCAAC ATTCGAGATA TCCCTGCAGT CTCTACAACT 
CTGATAGACA GCATTAAGGA TGTGGTGCAA AGTAAGACTG TTGTACCAAC ACCTTCAGCA 
ACAGATGTTA CAGATATATC AGTTTTCCCT ACAGTTATTG AAGATGTAAA TATCACTGTT 
GATTCAAGAA TATACACTTG TGACCAAGAA TTTACTGCAG ACATAGTATT TATAATAGAT 
ACTTCTCGTC ACAAACCTTC TGAAAGTGAA AACTTAAAGA AATTTCTTTC TGATGTCGTA 
TCAACGCTAC CAATAAGTGA AAACTGTGCC CATGTGCATT TGGTTAAATA TGGCAGCAAA 
GCTGAATTTC TGGCCCCACT AGAGACAGGA GTAAACAAAA GCCGTGTGTT GGAGGCCATA 
AAATCCATAA CTCCAATGAA AGAAGAGGCA GCTAACACTG GCCACGCTCT TAACTTTACA 
AGAACTGAAG TTGTTGCTGA TCCAAAGACA AGTAGGAAGG GACAAGGAAT AAAGCAAATT 
AGTATTTTGA TCACTCATAG TCCATCTGCA GATAATGTCA CTGATGCTGC GCATCTACTG 
CGACAAGACA GAGTAACAAG CTTTGCCTTG GGGATTGCAG AAGCCAAAAA AAATCAAATG 
GACCAAATAG CATCCTACCC ACCTGACAGT TATACTATAA GCTTAAAGGG ATTTTCAGAC 
CTTTCTACAC AGGTGGAATA TTTACAAAAA AATATTCAGA ATGAGCTGGA AGATTACACA 
GTACAATCCT ATGCAAGAAC AGGCCTTATT CAACAAGGAT GCCTGGACAC AGAGGCTGCT 
GATATCTATT TACTTGTTGA TGAGTCTGGC AGCATTACTA CTGACAACTT CCAAGAAATG 
AAGACATTTT TGAAAAAACT TGTAGAAATG TTTGACATAG GACCTGAAAA GGTTCTGGTT 
AGATGTGTAC TCTATTCAGA TTCACCAAGA GAGATTCCAA TCGGTAGAGA CTTTAGTAAA 
GATGAATTAA AATCTGCCCT GCAAAATCTA AGGCAATTAG GAGGTGGAAC AAATACTGGA 
AAAGCTATTA ATTTCACTCA GCAGGTAATT ACAAAAACAG AAAACACTAG GCCTGGGGAT 
GTGCCAGTAT ATCTGATTGT CCTTACAGAT GGAGAGTCCC AGGACAGTGT GAAGGAGGCT 
GCTGAGATTG TCCGGGGTCA TGGTGTAAAC ACTTATGCAA TTGGAGTAAA AGGGTATAAT 
AAAACTCAGT TGCTGGAGAT CACAGGAGCA GAGCACAGGG TTCACTTAGC CCACGACTTT 
GATTCCTTGA AACACATTAA GGATGTTATA GCACAGCAAA TCTGTTTTGG AAAAGCATGT 
CAAGACTTAA TGGCAGACAT TTTGTTTCTG GTTGATAGTT CCAGTGGTAT GGGATCAGAC 
AAGTTTAGCA AAATGAAAAC ATTCATGAAA GATCTGGTCA ACAAAACGGA GGTTGGATTG 
ACAGGTGTAC AGTTTGCAGC TGTTCAGTTC AGTAATTCAA TTAAGGAAGA ATTCCAGTTC 
AATAAACATG CAACCAAAAA TGCCATTTGG GACTCCATAG ACAACATGAA TTTAATGGGC 
AATGTTGCAA AGACTGGAAA TGCCTTAGCA AATGTAGCTG ACTACTTTAC AGAGGCTAAA 
GGAGCAAGAC CAAGTTCAAA AGTTAGTAAA ATTCTTCTAC TGATCACAGA TAATCCATCA 
CAAGATGAAG TAAAAGTTCC AGCCGATTCT TTAAGAAGCA ATGGATTGAT CATCTATTCT 
ATAGGTGGCT TCAGTGCAAA TAAAAAAGAA CTTCAAGAGA TAAGTGGAAA AATAACACCT 
TACTACCAGT CATTTGACAA ACTGCAGACA ATTGAAAATG ATTTGCTTTT TAGAATTTGC 
AATCCTGAAG AAGAATGCAA GAGAATAGAA GTGGCAGATA TTGTCTTTGT AATTGATTCA 
TCTGGCAGTA TAGACTATAC AGAATATAAG GAAATGCAAA ATTTTATGGT ATCGTTGGTG 
AACAAGTCAG CTGTTGGACC AGATAATGTT CAATTCGGCG CTCTCAAATA CTCTGATTAC 
AACACAGAAC TCTTCTACCT GAACAGGTAT ACAAACAAAG TGGACATTAT AAATCATATC 
AACAAAGATA CAACACAAGG TGGAAACACA TACACTGCAG GAGCGGTTAG GTTTTCAAAA 
GAGTTTTTCA CAGAGAAACA TGGAAGCCGC AAGGCAAGGG GAGTGCCTCA GATTGTAATG 
GTTATAACTG ATGGAGATTC TCATGATAAA GATAAACTGA ATGAGACAGC AAGGCAGCTA 
GAACAAGAAG GCATCATCAT CTATGCTATC GGCATTGATC AAGCAAACAC CAATGAGCTG 
GAAACATTGG CTGGGACAGA AGGCAAATGG TTCATGGTAG CTAACTTCAG TGGATTACAG 
GACATCCTTG TACAGGTATC AGAGGCAATG TGCAATAAAA CAGACTGCAA GGTTGAACAA 
GCAGATCTGT TGTTTCTTAT TGATGGATCC AGTAATACTT CTGAAGAAAC ATTCAGGGAA 
ATAAAAAATT TTGTGGTATC AGTTATGGAT GATTTTAATG TTGGCCCTGT AAATGTCCAT 
ATAGCTGTTT CTCAGTACAG TGAGTCTTGC ATTCGAGAAA TTAATTTTGA CTATTCTACT 
GAAAGAGGAA CTTTAAAGAA TGAAATAATA AACATCAGAA AGACCAAAGG GCGGCGTCAT 
ATTGGAGCAG CACTTGATTT CACAAAATCT ACCGTGTATA GCCCATCCAG TGATAACCGT 
TTAAACCAGG GTGTGAAGCA GCTTCTTGTA GTGATTACTG CTGGAAATGC ATCAGATCAA 
GTTGCACGAC CCGCGAAGGC TCTTAGGGAC CGTGGAGTCG ATATATATGC TGTGGGAATA 
GGAAATATTT GCAAGACACA GCTTACACAG ATAACTGGAT CACCTGAGAA GATATATACT 
GATGATGTCA GTGGCTTGAA AGCAATTAAA AAGAGGCTTG TTAGGGATAC ATGTTCAAAA 
GATCCTGTTA CCAATTGTTC CCTTGATGTT GTGGTCAGTT TCGACATCTC CACCCAGTCC 
AGAGGTCAAC GTTTATTCCA TGACCATCCT GAACTAGAAA CTATCCTGCC AAACGTTTTA 
AAGTCAATGG TGTCTCTGAA AAGCCCAAGC TGTGGTGCAG GAAAGCAGCC TCAAGCTTCT 
GTGGCATTTT ATGTGAAAGA TACAAACAGT GTTTCCAGAT TCAACACCTA CAGCCAAGAC 
CTATTTAATA AATTAAAGGA AGTTAAAGTT AGTGGCCCTT CATACCTTGA TTCTTTTTTT 
CTGAAGTCTT CATGGGATAT CTTTAAGAAT ACAGACAAAG GAAAAGCAAA GATGCTACTC 
GTGTTCACTG ATGGACTAGA TGAAGATGTA AAACAACTTG AATACACAGC AGGGTTTATT 
CGATCCCAAG GGCTGAATGC ATTAGTTACA GTTGCTTTGG AAGGAAGCAA AAAGGTGGAG 
GACATGAAGA TGATTGAATT TGGCAGAGGT TTTGAATATA AAAATCAGAT GCATGTTGGA 
ATGCCAGGCA TTGGTCTCAG GTTGGCCAGA CAAGCAGACC ATTCTGTTGA GACCATATGC 
TGCTGTGTAT TTTGTAAGTG CTATGGAGAA ACAGGTGAAC CAGGAGATGT TGGTGCTTAT 
GGAATAAAGG GAAGAAATGG GGCTAAAGGA CAGCAAGGAC ACAGAGGAGA TGATGGTGAA 
AGTGGTGAAA GAGGATTACT TGGTCCAACT GGTGAGCAAG GAATCAAGGG ATGCGATGGA 
GCCAGAGGAC GCAAGGGAAA CAGAGGATAT GCAGGAAGCA AAAGTGATGA TGGTGAGCCT 
GGACTTGAAG GATTGCCAGG AGAACAGGGG AACACTGGCC TTCCTGGGAC AAAAGGAGAA 
AAAGGAGTAC CCGGACAAGC AGGTAGTCCT GGTTCACGAG GAATTAAAGG AGACGAAGGA 
AATGAAGGCT TTCGAGGAGC TCCAGGTCAA CCTGGAACTA GTAGCTCTAT CCCTGGTCCC 
CCCGGTTTGA AAGGAGATCC AGGACAAGAG GGTGAAGCTG GGCGTCAGGG TCCACCGGGA 
CCTCCTGGAA GCAGAGGAAC AGGGAGTTTA CAAGGTAGAA GAGGCGTTCC AGGCCCAAAG 
GGAATAAAGG GAGAGCCTGG TGAGACTGGC TATGCTGGGG ATCAGGGAGA GCGAGGACCT 
CAGGGAGACA TTGGGAGCAG TGGATTAAAA GGTGAAAAAG GACTTAGTGG ATCCCAAGGA 
AATCGAGGAC CACCTGGTAT TGAAGGTTCT ACAGGGAGTC CAGGGAAACC TGGACCATTG 
GGGAGAAAGG GAGAGTCTGA AGGGCCTGGA CAAAAAGGAG AACCTGGAAC AGCTGGAAAA 
AGAGGACAGC CGGGTGAAGA TGGAAGATCT GGTTATGGTA AAGCAGGAAA AAAAGGAGTG 
AAGGGACAAC AAGGATTTCC AGGGGAAATA GGTGTTATGG GACCAAGAGG AGATGCTGGG 
ATGGATGGAG TAAAAGGTCT AAAGGGATAC AAAGGGAACC CGGGTCCTTC ATCTAAAGGG 
GAAGCTGGAA ATCGAGGAAT TCCCGGGTAC CCAGGCCGGA TGGGAAGAAA AGGAGACCGT 
GGGCCCTCAC CACCCTCTGC ATGTGAACTA ATTGAATTTG TACGTGATAA ATGCACATGT 
TGCCAAGGAA AACCTCCCTG CCCTGTGTAT CCTACAGAAT TAGTTTTTGC TCTAGAGATG 
TCAAGTGCAG TGACCCCAGC AATTTACACA CGAATGCTAA CACTGACAAA AGATATTGTG 
AGCAACATCA CAATCAGAGC AAGTAACTGT CCAGCAGGGG CTCGCGTGGC AGTTGTGTCC 
TATAATTCAA ATATAAAGCA CCTCATACGA TTCTCTGACT TTCAAATCAA GGACAAGTTG 
ATAAAGGCTG TGGAAAGTAT TCCTCTGGAA AAGTCCTCTC AAAGCCCTGA TGTTGGGGGT 
GCCATGACGT TTGTAGCAAG AAATATTTTT AAGAGATCCC TTCAAGGCTT CACAGTCAGA 
AGAATCGCTG TGCTTCTCAG CAGTGGACGT TCTGAAAATG CCAATAACAT CAACATGGCA 
GTCATGGAAT ATGGTGCTCT TGGTATCATT CCAGTGGTAT TTGCATTCAA CCCAGCACCT 
CTTATCAAGC GTGCCTTTGA AATGGATGCA ACAGGAGCTT TTGAACTGAT TGAAATGAAA 
CCAAATGTAG ATCCATTAAA AAATTTCAAG CTGTGCACTC TGTGTTTTGA CAAATGTCGG 
CCAGAAAAGT CCTGTGGCGA CAAATCAACT CAACAAAAGC CACCTATGGA TGTTGCCTTT 
CTTCTTGATA GTTCATACAA TGTGAAATAC GATGAATTTG AAGCAGCAAG AGAATTTTTA 
GGCAGCATGA TTGATCATTT TGACATTGAA GGATCAGCTT CTGATAGAGT AGCACTTGTG 
ACCTTTAATG AAAGTTATCA TGTTGACTTT AACTTATTAT CCTATGACAA CAAAAGTAGG 
ATCAAGAAAC ACATACAGGA AAGTGTTCAA CAGGTCAGTG GCCAACCAGC TCTTGGCTTA 
TCGCTACAAT ATACAATTGA GAACATTATG GCAAAGACAC CCAATCTAAG GAAACACAAA 
GTCATAATTA TTATATTATT TGGAGAGACA AGCCAGTGGG ACAAGCAGAC ACTCAGTGAT 
GCCTCTCAAA AAGCCAAATG TGAAGGCTAT GCTGTATTTG TGCTTTCCAT TGGTAGATCA 
CATAACAACA CTGAACTGAC AGAGCTTGCA AGTAAACCTT TGGATCATCA CTTACTTGGA 
CTTGGTAGAA TACACAAACC AGACCTTGTG TATGCCAGTG GGTTTCTGCA ACAATTTCTT 
AATTATGTGA GAAGAGGTAT TGGCAAGTAT CCCTCAGCCG AGCTCAAGAA GAAATGCTCA 
GCAGCTGCAG TGCCAGTCAG CAGAAGAAGA TTGCCCTCAC AGACTGATGC AGAAGAAATG 
AGAAGAGCAG GAGCTGAATA TCTTTCTGAT ATGCAGTTGG ACTTTATCTC TCAGAAACAA 
AGCAGCAACA TGGACAACTT AGCATTCCAG AACCTAGAAA GAGAGTTACT CAGGGCTGAC 
TCTGAAGAAG CCAAGACGGG CCACATCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031760280.1
CDS	154..6906
Translation	MKILLFKFLLFCWLQISFTQKAAVPEYADLVFLVDSSSNLGEKGFAQVKTLITRIISSLPVGPDKYRVGLAQYNDDLDIGFLLNTYKGKGPVISHIKNKLAYKGGSLKLGNALTKAHEKFFKEVSDRDKSVYPPVLVVVASGSSLDEVTQPALELQQDGVRIVSVGLQTAPLSQLQAIASSTRHVLQVSNIRDIPAVSTTLIDSIKDVVQSKTVVPTPSATDVTDISVFPTVIEDVNITVDSRIYTCDQEFTADIVFIIDTSRHKPSESENLKKFLSDVVSTLPISENCAHVHLVKYGSKAEFLAPLETGVNKSRVLEAIKSITPMKEEAANTGHALNFTRTEVVADPKTSRKGQGIKQISILITHSPSADNVTDAAHLLRQDRVTSFALGIAEAKKNQMDQIASYPPDSYTISLKGFSDLSTQVEYLQKNIQNELEDYTVQSYARTGLIQQGCLDTEAADIYLLVDESGSITTDNFQEMKTFLKKLVEMFDIGPEKVLVRCVLYSDSPREIPIGRDFSKDELKSALQNLRQLGGGTNTGKAINFTQQVITKTENTRPGDVPVYLIVLTDGESQDSVKEAAEIVRGHGVNTYAIGVKGYNKTQLLEITGAEHRVHLAHDFDSLKHIKDVIAQQICFGKACQDLMADILFLVDSSSGMGSDKFSKMKTFMKDLVNKTEVGLTGVQFAAVQFSNSIKEEFQFNKHATKNAIWDSIDNMNLMGNVAKTGNALANVADYFTEAKGARPSSKVSKILLLITDNPSQDEVKVPADSLRSNGLIIYSIGGFSANKKELQEISGKITPYYQSFDKLQTIENDLLFRICNPEEECKRIEVADIVFVIDSSGSIDYTEYKEMQNFMVSLVNKSAVGPDNVQFGALKYSDYNTELFYLNRYTNKVDIINHINKDTTQGGNTYTAGAVRFSKEFFTEKHGSRKARGVPQIVMVITDGDSHDKDKLNETARQLEQEGIIIYAIGIDQANTNELETLAGTEGKWFMVANFSGLQDILVQVSEAMCNKTDCKVEQADLLFLIDGSSNTSEETFREIKNFVVSVMDDFNVGPVNVHIAVSQYSESCIREINFDYSTERGTLKNEIINIRKTKGRRHIGAALDFTKSTVYSPSSDNRLNQGVKQLLVVITAGNASDQVARPAKALRDRGVDIYAVGIGNICKTQLTQITGSPEKIYTDDVSGLKAIKKRLVRDTCSKDPVTNCSLDVVVSFDISTQSRGQRLFHDHPELETILPNVLKSMVSLKSPSCGAGKQPQASVAFYVKDTNSVSRFNTYSQDLFNKLKEVKVSGPSYLDSFFLKSSWDIFKNTDKGKAKMLLVFTDGLDEDVKQLEYTAGFIRSQGLNALVTVALEGSKKVEDMKMIEFGRGFEYKNQMHVGMPGIGLRLARQADHSVETICCCVFCKCYGETGEPGDVGAYGIKGRNGAKGQQGHRGDDGESGERGLLGPTGEQGIKGCDGARGRKGNRGYAGSKSDDGEPGLEGLPGEQGNTGLPGTKGEKGVPGQAGSPGSRGIKGDEGNEGFRGAPGQPGTSSSIPGPPGLKGDPGQEGEAGRQGPPGPPGSRGTGSLQGRRGVPGPKGIKGEPGETGYAGDQGERGPQGDIGSSGLKGEKGLSGSQGNRGPPGIEGSTGSPGKPGPLGRKGESEGPGQKGEPGTAGKRGQPGEDGRSGYGKAGKKGVKGQQGFPGEIGVMGPRGDAGMDGVKGLKGYKGNPGPSSKGEAGNRGIPGYPGRMGRKGDRGPSPPSACELIEFVRDKCTCCQGKPPCPVYPTELVFALEMSSAVTPAIYTRMLTLTKDIVSNITIRASNCPAGARVAVVSYNSNIKHLIRFSDFQIKDKLIKAVESIPLEKSSQSPDVGGAMTFVARNIFKRSLQGFTVRRIAVLLSSGRSENANNINMAVMEYGALGIIPVVFAFNPAPLIKRAFEMDATGAFELIEMKPNVDPLKNFKLCTLCFDKCRPEKSCGDKSTQQKPPMDVAFLLDSSYNVKYDEFEAAREFLGSMIDHFDIEGSASDRVALVTFNESYHVDFNLLSYDNKSRIKKHIQESVQQVSGQPALGLSLQYTIENIMAKTPNLRKHKVIIIILFGETSQWDKQTLSDASQKAKCEGYAVFVLSIGRSHNNTELTELASKPLDHHLLGLGRIHKPDLVYASGFLQQFLNYVRRGIGKYPSAELKKKCSAAAVPVSRRRLPSQTDAEEMRRAGAEYLSDMQLDFISQKQSSNMDNLAFQNLERELLRADSEEAKTGHIR

Target ORF information:

RefSeq Version	XM_031904420.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis collagen, type VI, alpha 6 (col6a6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031904420.1

ORF Insert Sequence:

ATGAAGATCC TACTCTTCAA GTTTCTTTTG TTCTGCTGGC TTCAGATATC TTTTACTCAG 
AAAGCAGCTG TACCTGAGTA TGCAGATCTT GTCTTTCTGG TTGACAGTTC CAGTAACCTG 
GGAGAGAAGG GCTTCGCCCA AGTGAAAACC TTAATTACCA GAATAATCTC TTCATTGCCT 
GTAGGACCAG ACAAATATCG GGTTGGGCTG GCACAATACA ATGATGATTT AGACATTGGT 
TTTCTGCTAA ACACCTACAA GGGAAAGGGC CCAGTTATAA GTCATATAAA AAATAAACTT 
GCATATAAAG GTGGATCTCT AAAGCTTGGC AATGCACTCA CAAAAGCACA TGAAAAGTTC 
TTTAAAGAAG TCAGTGATAG AGACAAATCA GTGTATCCAC CAGTTCTAGT TGTTGTTGCT 
TCTGGATCGT CTCTGGATGA AGTTACCCAG CCAGCCCTTG AGCTTCAACA GGATGGAGTC 
AGAATAGTTT CAGTAGGGTT ACAAACAGCC CCCTTGTCTC AACTGCAAGC CATAGCTTCA 
TCCACTAGGC ATGTCTTACA GGTATCCAAC ATTCGAGATA TCCCTGCAGT CTCTACAACT 
CTGATAGACA GCATTAAGGA TGTGGTGCAA AGTAAGACTG TTGTACCAAC ACCTTCAGCA 
ACAGATGTTA CAGATATATC AGTTTTCCCT ACAGTTATTG AAGATGTAAA TATCACTGTT 
GATTCAAGAA TATACACTTG TGACCAAGAA TTTACTGCAG ACATAGTATT TATAATAGAT 
ACTTCTCGTC ACAAACCTTC TGAAAGTGAA AACTTAAAGA AATTTCTTTC TGATGTCGTA 
TCAACGCTAC CAATAAGTGA AAACTGTGCC CATGTGCATT TGGTTAAATA TGGCAGCAAA 
GCTGAATTTC TGGCCCCACT AGAGACAGGA GTAAACAAAA GCCGTGTGTT GGAGGCCATA 
AAATCCATAA CTCCAATGAA AGAAGAGGCA GCTAACACTG GCCACGCTCT TAACTTTACA 
AGAACTGAAG TTGTTGCTGA TCCAAAGACA AGTAGGAAGG GACAAGGAAT AAAGCAAATT 
AGTATTTTGA TCACTCATAG TCCATCTGCA GATAATGTCA CTGATGCTGC GCATCTACTG 
CGACAAGACA GAGTAACAAG CTTTGCCTTG GGGATTGCAG AAGCCAAAAA AAATCAAATG 
GACCAAATAG CATCCTACCC ACCTGACAGT TATACTATAA GCTTAAAGGG ATTTTCAGAC 
CTTTCTACAC AGGTGGAATA TTTACAAAAA AATATTCAGA ATGAGCTGGA AGATTACACA 
GTACAATCCT ATGCAAGAAC AGGCCTTATT CAACAAGGAT GCCTGGACAC AGAGGCTGCT 
GATATCTATT TACTTGTTGA TGAGTCTGGC AGCATTACTA CTGACAACTT CCAAGAAATG 
AAGACATTTT TGAAAAAACT TGTAGAAATG TTTGACATAG GACCTGAAAA GGTTCTGGTT 
AGATGTGTAC TCTATTCAGA TTCACCAAGA GAGATTCCAA TCGGTAGAGA CTTTAGTAAA 
GATGAATTAA AATCTGCCCT GCAAAATCTA AGGCAATTAG GAGGTGGAAC AAATACTGGA 
AAAGCTATTA ATTTCACTCA GCAGGTAATT ACAAAAACAG AAAACACTAG GCCTGGGGAT 
GTGCCAGTAT ATCTGATTGT CCTTACAGAT GGAGAGTCCC AGGACAGTGT GAAGGAGGCT 
GCTGAGATTG TCCGGGGTCA TGGTGTAAAC ACTTATGCAA TTGGAGTAAA AGGGTATAAT 
AAAACTCAGT TGCTGGAGAT CACAGGAGCA GAGCACAGGG TTCACTTAGC CCACGACTTT 
GATTCCTTGA AACACATTAA GGATGTTATA GCACAGCAAA TCTGTTTTGG AAAAGCATGT 
CAAGACTTAA TGGCAGACAT TTTGTTTCTG GTTGATAGTT CCAGTGGTAT GGGATCAGAC 
AAGTTTAGCA AAATGAAAAC ATTCATGAAA GATCTGGTCA ACAAAACGGA GGTTGGATTG 
ACAGGTGTAC AGTTTGCAGC TGTTCAGTTC AGTAATTCAA TTAAGGAAGA ATTCCAGTTC 
AATAAACATG CAACCAAAAA TGCCATTTGG GACTCCATAG ACAACATGAA TTTAATGGGC 
AATGTTGCAA AGACTGGAAA TGCCTTAGCA AATGTAGCTG ACTACTTTAC AGAGGCTAAA 
GGAGCAAGAC CAAGTTCAAA AGTTAGTAAA ATTCTTCTAC TGATCACAGA TAATCCATCA 
CAAGATGAAG TAAAAGTTCC AGCCGATTCT TTAAGAAGCA ATGGATTGAT CATCTATTCT 
ATAGGTGGCT TCAGTGCAAA TAAAAAAGAA CTTCAAGAGA TAAGTGGAAA AATAACACCT 
TACTACCAGT CATTTGACAA ACTGCAGACA ATTGAAAATG ATTTGCTTTT TAGAATTTGC 
AATCCTGAAG AAGAATGCAA GAGAATAGAA GTGGCAGATA TTGTCTTTGT AATTGATTCA 
TCTGGCAGTA TAGACTATAC AGAATATAAG GAAATGCAAA ATTTTATGGT ATCGTTGGTG 
AACAAGTCAG CTGTTGGACC AGATAATGTT CAATTCGGCG CTCTCAAATA CTCTGATTAC 
AACACAGAAC TCTTCTACCT GAACAGGTAT ACAAACAAAG TGGACATTAT AAATCATATC 
AACAAAGATA CAACACAAGG TGGAAACACA TACACTGCAG GAGCGGTTAG GTTTTCAAAA 
GAGTTTTTCA CAGAGAAACA TGGAAGCCGC AAGGCAAGGG GAGTGCCTCA GATTGTAATG 
GTTATAACTG ATGGAGATTC TCATGATAAA GATAAACTGA ATGAGACAGC AAGGCAGCTA 
GAACAAGAAG GCATCATCAT CTATGCTATC GGCATTGATC AAGCAAACAC CAATGAGCTG 
GAAACATTGG CTGGGACAGA AGGCAAATGG TTCATGGTAG CTAACTTCAG TGGATTACAG 
GACATCCTTG TACAGGTATC AGAGGCAATG TGCAATAAAA CAGACTGCAA GGTTGAACAA 
GCAGATCTGT TGTTTCTTAT TGATGGATCC AGTAATACTT CTGAAGAAAC ATTCAGGGAA 
ATAAAAAATT TTGTGGTATC AGTTATGGAT GATTTTAATG TTGGCCCTGT AAATGTCCAT 
ATAGCTGTTT CTCAGTACAG TGAGTCTTGC ATTCGAGAAA TTAATTTTGA CTATTCTACT 
GAAAGAGGAA CTTTAAAGAA TGAAATAATA AACATCAGAA AGACCAAAGG GCGGCGTCAT 
ATTGGAGCAG CACTTGATTT CACAAAATCT ACCGTGTATA GCCCATCCAG TGATAACCGT 
TTAAACCAGG GTGTGAAGCA GCTTCTTGTA GTGATTACTG CTGGAAATGC ATCAGATCAA 
GTTGCACGAC CCGCGAAGGC TCTTAGGGAC CGTGGAGTCG ATATATATGC TGTGGGAATA 
GGAAATATTT GCAAGACACA GCTTACACAG ATAACTGGAT CACCTGAGAA GATATATACT 
GATGATGTCA GTGGCTTGAA AGCAATTAAA AAGAGGCTTG TTAGGGATAC ATGTTCAAAA 
GATCCTGTTA CCAATTGTTC CCTTGATGTT GTGGTCAGTT TCGACATCTC CACCCAGTCC 
AGAGGTCAAC GTTTATTCCA TGACCATCCT GAACTAGAAA CTATCCTGCC AAACGTTTTA 
AAGTCAATGG TGTCTCTGAA AAGCCCAAGC TGTGGTGCAG GAAAGCAGCC TCAAGCTTCT 
GTGGCATTTT ATGTGAAAGA TACAAACAGT GTTTCCAGAT TCAACACCTA CAGCCAAGAC 
CTATTTAATA AATTAAAGGA AGTTAAAGTT AGTGGCCCTT CATACCTTGA TTCTTTTTTT 
CTGAAGTCTT CATGGGATAT CTTTAAGAAT ACAGACAAAG GAAAAGCAAA GATGCTACTC 
GTGTTCACTG ATGGACTAGA TGAAGATGTA AAACAACTTG AATACACAGC AGGGTTTATT 
CGATCCCAAG GGCTGAATGC ATTAGTTACA GTTGCTTTGG AAGGAAGCAA AAAGGTGGAG 
GACATGAAGA TGATTGAATT TGGCAGAGGT TTTGAATATA AAAATCAGAT GCATGTTGGA 
ATGCCAGGCA TTGGTCTCAG GTTGGCCAGA CAAGCAGACC ATTCTGTTGA GACCATATGC 
TGCTGTGTAT TTTGTAAGTG CTATGGAGAA ACAGGTGAAC CAGGAGATGT TGGTGCTTAT 
GGAATAAAGG GAAGAAATGG GGCTAAAGGA CAGCAAGGAC ACAGAGGAGA TGATGGTGAA 
AGTGGTGAAA GAGGATTACT TGGTCCAACT GGTGAGCAAG GAATCAAGGG ATGCGATGGA 
GCCAGAGGAC GCAAGGGAAA CAGAGGATAT GCAGGAAGCA AAAGTGATGA TGGTGAGCCT 
GGACTTGAAG GATTGCCAGG AGAACAGGGG AACACTGGCC TTCCTGGGAC AAAAGGAGAA 
AAAGGAGTAC CCGGACAAGC AGGTAGTCCT GGTTCACGAG GAATTAAAGG AGACGAAGGA 
AATGAAGGCT TTCGAGGAGC TCCAGGTCAA CCTGGAACTA GTAGCTCTAT CCCTGGTCCC 
CCCGGTTTGA AAGGAGATCC AGGACAAGAG GGTGAAGCTG GGCGTCAGGG TCCACCGGGA 
CCTCCTGGAA GCAGAGGAAC AGGGAGTTTA CAAGGTAGAA GAGGCGTTCC AGGCCCAAAG 
GGAATAAAGG GAGAGCCTGG TGAGACTGGC TATGCTGGGG ATCAGGGAGA GCGAGGACCT 
CAGGGAGACA TTGGGAGCAG TGGATTAAAA GGTGAAAAAG GACTTAGTGG ATCCCAAGGA 
AATCGAGGAC CACCTGGTAT TGAAGGTTCT ACAGGGAGTC CAGGGAAACC TGGACCATTG 
GGGAGAAAGG GAGAGTCTGA AGGGCCTGGA CAAAAAGGAG AACCTGGAAC AGCTGGAAAA 
AGAGGACAGC CGGGTGAAGA TGGAAGATCT GGTTATGGTA AAGCAGGAAA AAAAGGAGTG 
AAGGGACAAC AAGGATTTCC AGGGGAAATA GGTGTTATGG GACCAAGAGG AGATGCTGGG 
ATGGATGGAG TAAAAGGTCT AAAGGGATAC AAAGGGAACC CGGGTCCTTC ATCTAAAGGG 
GAAGCTGGAA ATCGAGGAAT TCCCGGGTAC CCAGGCCGGA TGGGAAGAAA AGGAGACCGT 
GGGCCCTCAC CACCCTCTGC ATGTGAACTA ATTGAATTTG TACGTGATAA ATGCACATGT 
TGCCAAGGAA AACCTCCCTG CCCTGTGTAT CCTACAGAAT TAGTTTTTGC TCTAGAGATG 
TCAAGTGCAG TGACCCCAGC AATTTACACA CGAATGCTAA CACTGACAAA AGATATTGTG 
AGCAACATCA CAATCAGAGC AAGTAACTGT CCAGCAGGGG CTCGCGTGGC AGTTGTGTCC 
TATAATTCAA ATATAAAGCA CCTCATACGA TTCTCTGACT TTCAAATCAA GGACAAGTTG 
ATAAAGGCTG TGGAAAGTAT TCCTCTGGAA AAGTCCTCTC AAAGCCCTGA TGTTGGGGGT 
GCCATGACGT TTGTAGCAAG AAATATTTTT AAGAGATCCC TTCAAGGCTT CACAGTCAGA 
AGAATCGCTG TGCTTCTCAG CAGTGGACGT TCTGAAAATG CCAATAACAT CAACATGGCA 
GTCATGGAAT ATGGTGCTCT TGGTATCATT CCAGTGGTAT TTGCATTCAA CCCAGCACCT 
CTTATCAAGC GTGCCTTTGA AATGGATGCA ACAGGAGCTT TTGAACTGAT TGAAATGAAA 
CCAAATGTAG ATCCATTAAA AAATTTCAAG CTGTGCACTC TGTGTTTTGA CAAATGTCGG 
CCAGAAAAGT CCTGTGGCGA CAAATCAACT CAACAAAAGC CACCTATGGA TGTTGCCTTT 
CTTCTTGATA GTTCATACAA TGTGAAATAC GATGAATTTG AAGCAGCAAG AGAATTTTTA 
GGCAGCATGA TTGATCATTT TGACATTGAA GGATCAGCTT CTGATAGAGT AGCACTTGTG 
ACCTTTAATG AAAGTTATCA TGTTGACTTT AACTTATTAT CCTATGACAA CAAAAGTAGG 
ATCAAGAAAC ACATACAGGA AAGTGTTCAA CAGGTCAGTG GCCAACCAGC TCTTGGCTTA 
TCGCTACAAT ATACAATTGA GAACATTATG GCAAAGACAC CCAATCTAAG GAAACACAAA 
GTCATAATTA TTATATTATT TGGAGAGACA AGCCAGTGGG ACAAGCAGAC ACTCAGTGAT 
GCCTCTCAAA AAGCCAAATG TGAAGGCTAT GCTGTATTTG TGCTTTCCAT TGGTAGATCA 
CATAACAACA CTGAACTGAC AGAGCTTGCA AGTAAACCTT TGGATCATCA CTTACTTGGA 
CTTGGTAGAA TACACAAACC AGACCTTGTG TATGCCAGTG GGTTTCTGCA ACAATTTCTT 
AATTATGTGA GAAGAGGTAT TGGCAAGTAT CCCTCAGCCG AGCTCAAGAA GAAATGCTCA 
GCAGCTGCAG TGCCAGTCAG CAGAAGAAGA TTGCCCTCAC AGACTGATGC AGAAGAAATG 
AGAAGAGCAG GAGCTGAATA TCTTTCTGAT ATGCAGTTGG ACTTTATCTC TCAGAAACAA 
AGCAGCAACA TGGACAACTT AGCATTCCAG AACCTAGAAA GAGAGTTACT CAGGGCTGAC 
TCTGAAGAAG CCAAGACGGG CCACATCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col6a6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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