wnk2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnk2
Entrez Gene ID	100488072
Full Name	WNK lysine deficient protein kinase 2
Synonyms	prkwnk2,sdccag43
General protein information	Preferred Names WNK lysine deficient protein kinase 2 Names serine/threonine-protein kinase WNK2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901288.1	XP_031757148.1	serine/threonine-protein kinase WNK2 isoform X2
XM_031901291.1	XP_031757151.1	serine/threonine-protein kinase WNK2 isoform X5
XM_031901289.1	XP_031757149.1	serine/threonine-protein kinase WNK2 isoform X3
XM_031901290.1	XP_031757150.1	serine/threonine-protein kinase WNK2 isoform X4
XM_031901292.1	XP_031757152.1	serine/threonine-protein kinase WNK2 isoform X6
XM_018090162.1	XP_017945651.1	serine/threonine-protein kinase WNK2
XM_031901287.1	XP_031757147.1	serine/threonine-protein kinase WNK2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	WNK2	XP_003951524.1
Canis lupus familiaris (dog)	WNK2	XP_005616885.1
Xenopus tropicalis (tropical clawed frog)	wnk2	XP_002943109.2
Homo sapiens (human)	WNK2	NP_006639.3
	WNK2	XP_001108626.2
Bos taurus (cattle)	WNK2	XP_005210503.1
Mus musculus (house mouse)	Wnk2	NP_083637.2
Rattus norvegicus (Norway rat)	Wnk2	NP_001178485.1
Gallus gallus (chicken)	WNK2	XP_414326.4

Gene Ontology

The following wnk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa57686	XM_031901291.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57687	XM_031901292.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa57682	XM_031901287.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa57683	XM_031901288.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa57684	XM_031901289.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa57685	XM_031901290.1 Latest version!	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnk2 ( XM_031901291.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757151.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa57686
Clone ID Related Accession (Same CDS sequence)	XM_031901291.1
Accession Version	XM_031901291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC 
CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC 
AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT 
CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC 
CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG 
TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA 
CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC 
AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA 
TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT 
GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG 
TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA 
AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG 
CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757151.1
CDS	427..6291
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTPGIQVATGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQPHPVVQQIPAPQGICPMQSATHPFPQYQAPLAQVAVPQLKPLHIPPVPAQQRPVPATQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKASTQSPTSPNGQAMKIPSINVTSFHSQSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGWTVYHQTSERVTYKSSSKPRTRFLSGPVSLSIWSTLKRLCLGKDHSSRSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901291.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901291.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC 
CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC 
AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT 
CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC 
CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG 
TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA 
CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC 
AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA 
TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT 
GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG 
TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA 
AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG 
CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk2 ( XM_031901292.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757152.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.

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CloneID	OXa57687
Clone ID Related Accession (Same CDS sequence)	XM_031901292.1
Accession Version	XM_031901292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGGTTG TTCTGCCCCA ATATCCTCTG GATGTGGGAA CTGCCATGGG GGTTATGGTA 
CCTTCTACAA CAATGTCACC ACCAGTGCAG TTTCCAGCTG AATCTGCCCC TCTGCACCCA 
CCTCAGGAAC ATGTCACCCC GGTATCCTAT ATACACACAG ATGTACCTAT GACCCAACAG 
AGCACAACTC TTCAGTCTGA GGGTCATCAG CCCGAAACTA CACAGCCACC TGTCCATCAG 
AGTCAAACTC TCTACCAAGC CCCCCGGCCT TCTGTGGAGA CCAATGTAGA GGAGCCGACT 
CTGCAAGAGA ATCCAGTGAG TTCCGCACAA AGTTGTGATA ACTATGCAGG CTCAGATGCT 
GCGTCTGGTA GAGAGATGAG TGATGGTGGA GAAGGGGTTC ATGGGAAACA GGATGGAAAA 
CCAACCAAAA AGCATCACCG GAGGTCTTCC CGCACCCGCT CTCGCCAGGA GAAGGCCAAT 
AAACCAAAGC TGACTATTCT TAATGTGAGC AATACAGGTG ACAAGATGGT GGAATGCCAG 
TTGGAGACTC ACAACCATAA AATGGTGACT TTCAAATTTG ACTTGGATGG CGATGCACCA 
GATGAGATTG CCACGTACAT GGTGGAGAAT GGCTTTATTC TGCAGATGGA GAAGGAGGTG 
TTTATTGAGC AAATGAAGGA TGTTATGGAC AAAGCGGAAG ACATGTTGAG CGAAGACACT 
GAAGGGGAGA GAAGTTCAGA CCAAGCGGCG AGTCCTCAGA AAGTGGATTC TAATGGGAGA 
GAGACGAGGG AGGAAAACAC CCCAAATCAA ATGAAGACTC CGGTCTATCA GCAAAACGTT 
CTACATACTG GGAAGCGATG GTTTATTATA TGTCCAGTGG CCGAAACCAC CGGTTCCTCC 
CTGCCAGAAG TGCCCCAAAC TCCCTTAAAT ACCCAGAAGC CGACTGACGT TAAAGATCAG 
ACCGCCTGCG AGGATTCAGT ATTTGCCACA AATGCGGATA AGTTGGACTT TGCGGTGCCG 
CCGGGCATAC TGTGTGAGAG TGCCAGTGCC ATTCCTGCCT CAAGGACACA ATCTTCTTCT 
ACAATATCTT CCTCCGATCT GCAAACCCAA ACGGAACTGT CCTTTTCTGC TCTTCCTGAG 
TCTCTCTCCT TTCTCCAAGA AGAGGAACCG GTCCTGTTCA GCCCGCCGGC AGTTACGCAG 
CCTCTAATGG ACTCCATAAG CCATGAGGCC GGAGGGAGCC TGGCGCCTGT ATTCTCCGCA 
GCACAAGAAC CCGAGCAGAA ACCCAGTCCT ACAGATTCAG TAAATTCAAC AGAACCGCCA 
CGTGCAGCCT TACCCCTCTC CGACCACGAA CGACCGGCCG CTCAGCAAGA ACGGACTCTA 
GATCCACCCC CAGAGCGGAG TACATTGTAT GGATCCAGTA TTGGTTTCCC CGTTGTAAAC 
TCCATAAAAC CAGATGCCCC CTTGCCCATA CCCCCTTGCC AGCCACACCT TGATTCTGAA 
ATTTCCACCC CACTGAAACC AGTCCCAGTC CCCACTCAGT CTCTGGTTTT ACCAGTTGGC 
GGCACATCTT GCCAACAGCC CAGTATTACC GAGTCGGACG GGGAAGGCCC CCCGAGGGTA 
GAATTTACCG ACGATACCAT TAAAAGCTTA GATGAAAAGC TCCGCACTCT CTTGTACCAG 
GAATACACCC CGGCCAACGT TTCTGCCGGA ACCCCTGACA ATTGTTCCGG AGCAGAAAGC 
GGAGCAGAGA CAACGTTGGG CAACGGAGAA GAGATTGCTG AACACAGAAA CCTCTCCCAG 
TCTGTCTCTG CGCCACTAGA GAAAGCAACG GAACTGGGCT GGACTGTCGC AGAGAAAAGT 
TCTTGTGAGC TGTCAAACCA AAAGATTCAG GCTGCACCTC TGAACCATTC CCAGGGGTGG 
GATTCTGGCA AAGAAAGTGC AGTTGACTCT TCTTCCAAAA AGAGAGAGAC CCCTGACAAG 
TCCTCCAAGA CCATCGGCAG GTTCTCGGTT GTGAGTGCAG AAGATGATGC CCCAGTGACC 
GCTCCAAATT ACACGAGATT TTCAGCCCCG CCCGATGTCT ACCTGGATCA GGCTCCACCA 
CAACCAGCCA GGGCCTCTGT CCCTAGAGCT CACACCTCTT CCCCTATTAA CTCTCTGTTC 
TGTGGCCATG TTTCCAGTGA CTCTGGGGAT GATACCCCTC ACTCCAAGTC CAAAGAGGCT 
CAGCCGTCCA CCCCAAGTAA GGGGGGCACC AATGACTTCA TGAAGAAGGC CGCTGCTTTC 
TTGCATCGCT CTGGAAAGGC CAGTACCCAA AGCCCCACCT CTCCCAACGG CCAAGCCATG 
AAAATCCCTT CAATAAACGT GACCTCTTTC CATTCGCAGT CGTCCTATAT GAGCAGTGAT 
AACGACTCGG AGTTTGAAGA TGCCGACATG AAGAAGGAAC TGCAGAGCCT CCGGGAGAAG 
CACATGAAGG AAATAACAGA ACTGCAATCG CAGCAGAAGA AGGAGATTGA GGCCCTTTAC 
CTCCGGCTGG GGAAGCCCCT CCCACCCAAT GTGGGATTCC TGCACGCAGC ACCCCCCAGT 
GGCCGCCGGA GGAGGATCAG CAAAAATAAG CTGAAAACTG GGAAACTGCT CAATCCGCTG 
GTGCAGCAGC TGAAGAATTC GACTACCAGT CCAAGCCGCA TTTCCCCAGA ATGCAATGGT 
TCCCCTGCTA AGGATCCTGC AAAGTCTCTG GCAGACTGCA CCGCAGGAAC CTCGCCAAGC 
CGTGCCGTAC AAACGCAGCA GCCTTGCTCT GTGAAAGTCA CCATGTCGTC CGATAATATC 
TGCTCTGGGG TCACCCGGGA CGGTGTCGAT GTGCAGAGCA ATTCAAGCCA AGGCTGGACG 
GTTTACCACC AAACGTCTGA GAGAGTGACC TATAAATCTA GTAGCAAACC TCGTACCAGA 
TTCCTCAGTG GACCTGTGTC TCTGTCCATC TGGTCCACTC TGAAACGCCT GTGTCTAGGC 
AAAGATCACA GTAGTAGATC TTCGACAAGC AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG 
CTGCCCCCGC TGAACGCACA AATCCAGCCG CAGGCCAACA ACAGCAACAA CAACAAAAAG 
GGGACGTTTA CGGATGACCT TCACAAATTG GTGGATGAGT GGGCCTGCAA AACCGTCGGC 
GTGGCACAAT TCAAACCCTC ATTAAACCAA TTGAAGCAGT ACCAGCAGCG GCAAGATCTG 
GAGCCCAAGT CTGTGCCCAG CGACCTGTGT GACATGAGCC GTGCCGTGCC AGAGCCCAGG 
AAGAGATACA GCATTCCCAT GGCTGCCCCT CCGGCTGTAC CACTGGCTCC CCTCCTCTCT 
GCCGCTGTCC CAGCAACTGT TCCAGGAGCC GTGCAGCCGG GTACCTTTGT CATGCCCTTT 
TGTCCGTACG CGGCACCAAT GTACGGCATG CCCTGGGCAG GAGCGGCTGA GCTTGGGACT 
GCAGGAAATC AGATCATGGC CCAAGTAGCC CCCATAGGCA ACCAAATATT CCCTGTGGCA 
CTGCAGCACC CAGGTTTGCA CCCTGCTGGA TCCAACATGC GGATTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757152.1
CDS	427..6117
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTPGIQVATGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKASTQSPTSPNGQAMKIPSINVTSFHSQSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGWTVYHQTSERVTYKSSSKPRTRFLSGPVSLSIWSTLKRLCLGKDHSSRSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901292.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901292.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGGTTG TTCTGCCCCA ATATCCTCTG GATGTGGGAA CTGCCATGGG GGTTATGGTA 
CCTTCTACAA CAATGTCACC ACCAGTGCAG TTTCCAGCTG AATCTGCCCC TCTGCACCCA 
CCTCAGGAAC ATGTCACCCC GGTATCCTAT ATACACACAG ATGTACCTAT GACCCAACAG 
AGCACAACTC TTCAGTCTGA GGGTCATCAG CCCGAAACTA CACAGCCACC TGTCCATCAG 
AGTCAAACTC TCTACCAAGC CCCCCGGCCT TCTGTGGAGA CCAATGTAGA GGAGCCGACT 
CTGCAAGAGA ATCCAGTGAG TTCCGCACAA AGTTGTGATA ACTATGCAGG CTCAGATGCT 
GCGTCTGGTA GAGAGATGAG TGATGGTGGA GAAGGGGTTC ATGGGAAACA GGATGGAAAA 
CCAACCAAAA AGCATCACCG GAGGTCTTCC CGCACCCGCT CTCGCCAGGA GAAGGCCAAT 
AAACCAAAGC TGACTATTCT TAATGTGAGC AATACAGGTG ACAAGATGGT GGAATGCCAG 
TTGGAGACTC ACAACCATAA AATGGTGACT TTCAAATTTG ACTTGGATGG CGATGCACCA 
GATGAGATTG CCACGTACAT GGTGGAGAAT GGCTTTATTC TGCAGATGGA GAAGGAGGTG 
TTTATTGAGC AAATGAAGGA TGTTATGGAC AAAGCGGAAG ACATGTTGAG CGAAGACACT 
GAAGGGGAGA GAAGTTCAGA CCAAGCGGCG AGTCCTCAGA AAGTGGATTC TAATGGGAGA 
GAGACGAGGG AGGAAAACAC CCCAAATCAA ATGAAGACTC CGGTCTATCA GCAAAACGTT 
CTACATACTG GGAAGCGATG GTTTATTATA TGTCCAGTGG CCGAAACCAC CGGTTCCTCC 
CTGCCAGAAG TGCCCCAAAC TCCCTTAAAT ACCCAGAAGC CGACTGACGT TAAAGATCAG 
ACCGCCTGCG AGGATTCAGT ATTTGCCACA AATGCGGATA AGTTGGACTT TGCGGTGCCG 
CCGGGCATAC TGTGTGAGAG TGCCAGTGCC ATTCCTGCCT CAAGGACACA ATCTTCTTCT 
ACAATATCTT CCTCCGATCT GCAAACCCAA ACGGAACTGT CCTTTTCTGC TCTTCCTGAG 
TCTCTCTCCT TTCTCCAAGA AGAGGAACCG GTCCTGTTCA GCCCGCCGGC AGTTACGCAG 
CCTCTAATGG ACTCCATAAG CCATGAGGCC GGAGGGAGCC TGGCGCCTGT ATTCTCCGCA 
GCACAAGAAC CCGAGCAGAA ACCCAGTCCT ACAGATTCAG TAAATTCAAC AGAACCGCCA 
CGTGCAGCCT TACCCCTCTC CGACCACGAA CGACCGGCCG CTCAGCAAGA ACGGACTCTA 
GATCCACCCC CAGAGCGGAG TACATTGTAT GGATCCAGTA TTGGTTTCCC CGTTGTAAAC 
TCCATAAAAC CAGATGCCCC CTTGCCCATA CCCCCTTGCC AGCCACACCT TGATTCTGAA 
ATTTCCACCC CACTGAAACC AGTCCCAGTC CCCACTCAGT CTCTGGTTTT ACCAGTTGGC 
GGCACATCTT GCCAACAGCC CAGTATTACC GAGTCGGACG GGGAAGGCCC CCCGAGGGTA 
GAATTTACCG ACGATACCAT TAAAAGCTTA GATGAAAAGC TCCGCACTCT CTTGTACCAG 
GAATACACCC CGGCCAACGT TTCTGCCGGA ACCCCTGACA ATTGTTCCGG AGCAGAAAGC 
GGAGCAGAGA CAACGTTGGG CAACGGAGAA GAGATTGCTG AACACAGAAA CCTCTCCCAG 
TCTGTCTCTG CGCCACTAGA GAAAGCAACG GAACTGGGCT GGACTGTCGC AGAGAAAAGT 
TCTTGTGAGC TGTCAAACCA AAAGATTCAG GCTGCACCTC TGAACCATTC CCAGGGGTGG 
GATTCTGGCA AAGAAAGTGC AGTTGACTCT TCTTCCAAAA AGAGAGAGAC CCCTGACAAG 
TCCTCCAAGA CCATCGGCAG GTTCTCGGTT GTGAGTGCAG AAGATGATGC CCCAGTGACC 
GCTCCAAATT ACACGAGATT TTCAGCCCCG CCCGATGTCT ACCTGGATCA GGCTCCACCA 
CAACCAGCCA GGGCCTCTGT CCCTAGAGCT CACACCTCTT CCCCTATTAA CTCTCTGTTC 
TGTGGCCATG TTTCCAGTGA CTCTGGGGAT GATACCCCTC ACTCCAAGTC CAAAGAGGCT 
CAGCCGTCCA CCCCAAGTAA GGGGGGCACC AATGACTTCA TGAAGAAGGC CGCTGCTTTC 
TTGCATCGCT CTGGAAAGGC CAGTACCCAA AGCCCCACCT CTCCCAACGG CCAAGCCATG 
AAAATCCCTT CAATAAACGT GACCTCTTTC CATTCGCAGT CGTCCTATAT GAGCAGTGAT 
AACGACTCGG AGTTTGAAGA TGCCGACATG AAGAAGGAAC TGCAGAGCCT CCGGGAGAAG 
CACATGAAGG AAATAACAGA ACTGCAATCG CAGCAGAAGA AGGAGATTGA GGCCCTTTAC 
CTCCGGCTGG GGAAGCCCCT CCCACCCAAT GTGGGATTCC TGCACGCAGC ACCCCCCAGT 
GGCCGCCGGA GGAGGATCAG CAAAAATAAG CTGAAAACTG GGAAACTGCT CAATCCGCTG 
GTGCAGCAGC TGAAGAATTC GACTACCAGT CCAAGCCGCA TTTCCCCAGA ATGCAATGGT 
TCCCCTGCTA AGGATCCTGC AAAGTCTCTG GCAGACTGCA CCGCAGGAAC CTCGCCAAGC 
CGTGCCGTAC AAACGCAGCA GCCTTGCTCT GTGAAAGTCA CCATGTCGTC CGATAATATC 
TGCTCTGGGG TCACCCGGGA CGGTGTCGAT GTGCAGAGCA ATTCAAGCCA AGGCTGGACG 
GTTTACCACC AAACGTCTGA GAGAGTGACC TATAAATCTA GTAGCAAACC TCGTACCAGA 
TTCCTCAGTG GACCTGTGTC TCTGTCCATC TGGTCCACTC TGAAACGCCT GTGTCTAGGC 
AAAGATCACA GTAGTAGATC TTCGACAAGC AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG 
CTGCCCCCGC TGAACGCACA AATCCAGCCG CAGGCCAACA ACAGCAACAA CAACAAAAAG 
GGGACGTTTA CGGATGACCT TCACAAATTG GTGGATGAGT GGGCCTGCAA AACCGTCGGC 
GTGGCACAAT TCAAACCCTC ATTAAACCAA TTGAAGCAGT ACCAGCAGCG GCAAGATCTG 
GAGCCCAAGT CTGTGCCCAG CGACCTGTGT GACATGAGCC GTGCCGTGCC AGAGCCCAGG 
AAGAGATACA GCATTCCCAT GGCTGCCCCT CCGGCTGTAC CACTGGCTCC CCTCCTCTCT 
GCCGCTGTCC CAGCAACTGT TCCAGGAGCC GTGCAGCCGG GTACCTTTGT CATGCCCTTT 
TGTCCGTACG CGGCACCAAT GTACGGCATG CCCTGGGCAG GAGCGGCTGA GCTTGGGACT 
GCAGGAAATC AGATCATGGC CCAAGTAGCC CCCATAGGCA ACCAAATATT CCCTGTGGCA 
CTGCAGCACC CAGGTTTGCA CCCTGCTGGA TCCAACATGC GGATTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk2 ( XM_031901287.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757147.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.

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CloneID	OXa57682
Clone ID Related Accession (Same CDS sequence)	XM_031901287.1
Accession Version	XM_031901287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC 
CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC 
AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT 
CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC 
CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG 
TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA 
CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC 
AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA 
TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT 
GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG 
TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA 
AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG 
CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757147.1
CDS	427..6771
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTPGIQVATGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQPHPVVQQIPAPQGICPMQSATHPFPQYQAPLAQVAVPQLKPLHIPPVPAQQRPVPATQLPPYPLIPAVTPLAGLETLPGASLSDFPAVCVTPLPAPCHYFPPGIVVASVPLTPAIAPPLPLQAMNSQLVLSYETNIVPNVPVGTVPLLAAAGVPTLPTPQAVSQHPQVQQFQTSYPPLIQSDVPSQNVSHQATVPPPQTHHPSIPHPSDQASAGACNQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKASTQSPTSPNGQAMKIPSINVTSFHSQSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGWTVYHQTSERVTYKSSSKPRTRFLSGPVSLSIWSTLKRLCLGKDHSSRSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901287.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901287.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC 
CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC 
AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT 
CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC 
CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG 
TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA 
CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC 
AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA 
TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT 
GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG 
TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA 
AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG 
CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk2 ( XM_031901288.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757148.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.

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CloneID	OXa57683
Clone ID Related Accession (Same CDS sequence)	XM_031901288.1
Accession Version	XM_031901288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGACTGGCAC CCTCCCGGGT 
CTTGGGCAGC CGCATTACCA AGACCCCGGT GCCCAGTACC CGATGATGCA AGCGAATGCC 
ATTCCTTCAG TGCAGTTTGC TCAGTTACAG CCAGTGTTAC CTGGACAGCC GCATCCGGTC 
GTACAGCAAA TCCCGGCACC TCAGGGCATC TGCCCCATGC AGTCAGCCAC TCACCCCTTC 
CCACAGTACC AAGCGCCCCT CGCCCAAGTG GCAGTGCCAC AACTGAAGCC TCTCCATATC 
CCTCCTGTGC CCGCACAGCA GCGCCCGGTT CCTGCCACCC AGCTTCCACC GTACCCTCTC 
ATTCCAGCAG TTACTCCTCT CGCAGGACTA GAGACTCTCC CTGGCGCCAG TCTCTCTGAC 
TTTCCCGCTG TCTGTGTTAC CCCATTACCT GCCCCCTGTC ACTACTTTCC CCCAGGCATT 
GTGGTGGCCA GTGTCCCATT GACTCCTGCC ATAGCCCCAC CCCTGCCTTT GCAAGCCATG 
AATTCGCAGT TGGTACTGTC CTATGAGACT AACATAGTCC CAAATGTGCC AGTGGGCACG 
GTTCCATTGC TAGCAGCAGC CGGTGTCCCA ACGTTACCCA CCCCACAGGC TGTTTCCCAG 
CATCCCCAAG TTCAGCAGTT CCAAACATCC TACCCACCCC TCATTCAGAG TGATGTTCCC 
TCTCAAAATG TCTCCCATCA AGCAACTGTT CCACCTCCTC AAACCCACCA TCCGAGCATC 
CCTCATCCCT CTGACCAGGC CTCGGCCGGG GCATGTAACC AGGTTGTTCT GCCCCAATAT 
CCTCTGGATG TGGGAACTGC CATGGGGGTT ATGGTACCTT CTACAACAAT GTCACCACCA 
GTGCAGTTTC CAGCTGAATC TGCCCCTCTG CACCCACCTC AGGAACATGT CACCCCGGTA 
TCCTATATAC ACACAGATGT ACCTATGACC CAACAGAGCA CAACTCTTCA GTCTGAGGGT 
CATCAGCCCG AAACTACACA GCCACCTGTC CATCAGAGTC AAACTCTCTA CCAAGCCCCC 
CGGCCTTCTG TGGAGACCAA TGTAGAGGAG CCGACTCTGC AAGAGAATCC AGTGAGTTCC 
GCACAAAGTT GTGATAACTA TGCAGGCTCA GATGCTGCGT CTGGTAGAGA GATGAGTGAT 
GGTGGAGAAG GGGTTCATGG GAAACAGGAT GGAAAACCAA CCAAAAAGCA TCACCGGAGG 
TCTTCCCGCA CCCGCTCTCG CCAGGAGAAG GCCAATAAAC CAAAGCTGAC TATTCTTAAT 
GTGAGCAATA CAGGTGACAA GATGGTGGAA TGCCAGTTGG AGACTCACAA CCATAAAATG 
GTGACTTTCA AATTTGACTT GGATGGCGAT GCACCAGATG AGATTGCCAC GTACATGGTG 
GAGAATGGCT TTATTCTGCA GATGGAGAAG GAGGTGTTTA TTGAGCAAAT GAAGGATGTT 
ATGGACAAAG CGGAAGACAT GTTGAGCGAA GACACTGAAG GGGAGAGAAG TTCAGACCAA 
GCGGCGAGTC CTCAGAAAGT GGATTCTAAT GGGAGAGAGA CGAGGGAGGA AAACACCCCA 
AATCAAATGA AGACTCCGGT CTATCAGCAA AACGTTCTAC ATACTGGGAA GCGATGGTTT 
ATTATATGTC CAGTGGCCGA AACCACCGGT TCCTCCCTGC CAGAAGTGCC CCAAACTCCC 
TTAAATACCC AGAAGCCGAC TGACGTTAAA GATCAGACCG CCTGCGAGGA TTCAGTATTT 
GCCACAAATG CGGATAAGTT GGACTTTGCG GTGCCGCCGG GCATACTGTG TGAGAGTGCC 
AGTGCCATTC CTGCCTCAAG GACACAATCT TCTTCTACAA TATCTTCCTC CGATCTGCAA 
ACCCAAACGG AACTGTCCTT TTCTGCTCTT CCTGAGTCTC TCTCCTTTCT CCAAGAAGAG 
GAACCGGTCC TGTTCAGCCC GCCGGCAGTT ACGCAGCCTC TAATGGACTC CATAAGCCAT 
GAGGCCGGAG GGAGCCTGGC GCCTGTATTC TCCGCAGCAC AAGAACCCGA GCAGAAACCC 
AGTCCTACAG ATTCAGTAAA TTCAACAGAA CCGCCACGTG CAGCCTTACC CCTCTCCGAC 
CACGAACGAC CGGCCGCTCA GCAAGAACGG ACTCTAGATC CACCCCCAGA GCGGAGTACA 
TTGTATGGAT CCAGTATTGG TTTCCCCGTT GTAAACTCCA TAAAACCAGA TGCCCCCTTG 
CCCATACCCC CTTGCCAGCC ACACCTTGAT TCTGAAATTT CCACCCCACT GAAACCAGTC 
CCAGTCCCCA CTCAGTCTCT GGTTTTACCA GTTGGCGGCA CATCTTGCCA ACAGCCCAGT 
ATTACCGAGT CGGACGGGGA AGGCCCCCCG AGGGTAGAAT TTACCGACGA TACCATTAAA 
AGCTTAGATG AAAAGCTCCG CACTCTCTTG TACCAGGAAT ACACCCCGGC CAACGTTTCT 
GCCGGAACCC CTGACAATTG TTCCGGAGCA GAAAGCGGAG CAGAGACAAC GTTGGGCAAC 
GGAGAAGAGA TTGCTGAACA CAGAAACCTC TCCCAGTCTG TCTCTGCGCC ACTAGAGAAA 
GCAACGGAAC TGGGCTGGAC TGTCGCAGAG AAAAGTTCTT GTGAGCTGTC AAACCAAAAG 
ATTCAGGCTG CACCTCTGAA CCATTCCCAG GGGTGGGATT CTGGCAAAGA AAGTGCAGTT 
GACTCTTCTT CCAAAAAGAG AGAGACCCCT GACAAGTCCT CCAAGACCAT CGGCAGGTTC 
TCGGTTGTGA GTGCAGAAGA TGATGCCCCA GTGACCGCTC CAAATTACAC GAGATTTTCA 
GCCCCGCCCG ATGTCTACCT GGATCAGGCT CCACCACAAC CAGCCAGGGC CTCTGTCCCT 
AGAGCTCACA CCTCTTCCCC TATTAACTCT CTGTTCTGTG GCCATGTTTC CAGTGACTCT 
GGGGATGATA CCCCTCACTC CAAGTCCAAA GAGGCTCAGC CGTCCACCCC AAGTAAGGGG 
GGCACCAATG ACTTCATGAA GAAGGCCGCT GCTTTCTTGC ATCGCTCTGG AAAGGCCAGT 
ACCCAAAGCC CCACCTCTCC CAACGGCCAA GCCATGAAAA TCCCTTCAAT AAACGTGACC 
TCTTTCCATT CGCAGTCGTC CTATATGAGC AGTGATAACG ACTCGGAGTT TGAAGATGCC 
GACATGAAGA AGGAACTGCA GAGCCTCCGG GAGAAGCACA TGAAGGAAAT AACAGAACTG 
CAATCGCAGC AGAAGAAGGA GATTGAGGCC CTTTACCTCC GGCTGGGGAA GCCCCTCCCA 
CCCAATGTGG GATTCCTGCA CGCAGCACCC CCCAGTGGCC GCCGGAGGAG GATCAGCAAA 
AATAAGCTGA AAACTGGGAA ACTGCTCAAT CCGCTGGTGC AGCAGCTGAA GAATTCGACT 
ACCAGTCCAA GCCGCATTTC CCCAGAATGC AATGGTTCCC CTGCTAAGGA TCCTGCAAAG 
TCTCTGGCAG ACTGCACCGC AGGAACCTCG CCAAGCCGTG CCGTACAAAC GCAGCAGCCT 
TGCTCTGTGA AAGTCACCAT GTCGTCCGAT AATATCTGCT CTGGGGTCAC CCGGGACGGT 
GTCGATGTGC AGAGCAATTC AAGCCAAGGC TGGACGGTTT ACCACCAAAC GTCTGAGAGA 
GTGACCTATA AATCTAGTAG CAAACCTCGT ACCAGATTCC TCAGTGGACC TGTGTCTCTG 
TCCATCTGGT CCACTCTGAA ACGCCTGTGT CTAGGCAAAG ATCACAGTAG TAGATCTTCG 
ACAAGCAGCC TCGCAACCGC CCCCGAGGCC TCGGCGCTGC CCCCGCTGAA CGCACAAATC 
CAGCCGCAGG CCAACAACAG CAACAACAAC AAAAAGGGGA CGTTTACGGA TGACCTTCAC 
AAATTGGTGG ATGAGTGGGC CTGCAAAACC GTCGGCGTGG CACAATTCAA ACCCTCATTA 
AACCAATTGA AGCAGTACCA GCAGCGGCAA GATCTGGAGC CCAAGTCTGT GCCCAGCGAC 
CTGTGTGACA TGAGCCGTGC CGTGCCAGAG CCCAGGAAGA GATACAGCAT TCCCATGGCT 
GCCCCTCCGG CTGTACCACT GGCTCCCCTC CTCTCTGCCG CTGTCCCAGC AACTGTTCCA 
GGAGCCGTGC AGCCGGGTAC CTTTGTCATG CCCTTTTGTC CGTACGCGGC ACCAATGTAC 
GGCATGCCCT GGGCAGGAGC GGCTGAGCTT GGGACTGCAG GAAATCAGAT CATGGCCCAA 
GTAGCCCCCA TAGGCAACCA AATATTCCCT GTGGCACTGC AGCACCCAGG TTTGCACCCT 
GCTGGATCCA ACATGCGGAT TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757148.1
CDS	427..6753
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTTGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQPHPVVQQIPAPQGICPMQSATHPFPQYQAPLAQVAVPQLKPLHIPPVPAQQRPVPATQLPPYPLIPAVTPLAGLETLPGASLSDFPAVCVTPLPAPCHYFPPGIVVASVPLTPAIAPPLPLQAMNSQLVLSYETNIVPNVPVGTVPLLAAAGVPTLPTPQAVSQHPQVQQFQTSYPPLIQSDVPSQNVSHQATVPPPQTHHPSIPHPSDQASAGACNQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKASTQSPTSPNGQAMKIPSINVTSFHSQSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGWTVYHQTSERVTYKSSSKPRTRFLSGPVSLSIWSTLKRLCLGKDHSSRSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901288.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901288.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGACTGGCAC CCTCCCGGGT 
CTTGGGCAGC CGCATTACCA AGACCCCGGT GCCCAGTACC CGATGATGCA AGCGAATGCC 
ATTCCTTCAG TGCAGTTTGC TCAGTTACAG CCAGTGTTAC CTGGACAGCC GCATCCGGTC 
GTACAGCAAA TCCCGGCACC TCAGGGCATC TGCCCCATGC AGTCAGCCAC TCACCCCTTC 
CCACAGTACC AAGCGCCCCT CGCCCAAGTG GCAGTGCCAC AACTGAAGCC TCTCCATATC 
CCTCCTGTGC CCGCACAGCA GCGCCCGGTT CCTGCCACCC AGCTTCCACC GTACCCTCTC 
ATTCCAGCAG TTACTCCTCT CGCAGGACTA GAGACTCTCC CTGGCGCCAG TCTCTCTGAC 
TTTCCCGCTG TCTGTGTTAC CCCATTACCT GCCCCCTGTC ACTACTTTCC CCCAGGCATT 
GTGGTGGCCA GTGTCCCATT GACTCCTGCC ATAGCCCCAC CCCTGCCTTT GCAAGCCATG 
AATTCGCAGT TGGTACTGTC CTATGAGACT AACATAGTCC CAAATGTGCC AGTGGGCACG 
GTTCCATTGC TAGCAGCAGC CGGTGTCCCA ACGTTACCCA CCCCACAGGC TGTTTCCCAG 
CATCCCCAAG TTCAGCAGTT CCAAACATCC TACCCACCCC TCATTCAGAG TGATGTTCCC 
TCTCAAAATG TCTCCCATCA AGCAACTGTT CCACCTCCTC AAACCCACCA TCCGAGCATC 
CCTCATCCCT CTGACCAGGC CTCGGCCGGG GCATGTAACC AGGTTGTTCT GCCCCAATAT 
CCTCTGGATG TGGGAACTGC CATGGGGGTT ATGGTACCTT CTACAACAAT GTCACCACCA 
GTGCAGTTTC CAGCTGAATC TGCCCCTCTG CACCCACCTC AGGAACATGT CACCCCGGTA 
TCCTATATAC ACACAGATGT ACCTATGACC CAACAGAGCA CAACTCTTCA GTCTGAGGGT 
CATCAGCCCG AAACTACACA GCCACCTGTC CATCAGAGTC AAACTCTCTA CCAAGCCCCC 
CGGCCTTCTG TGGAGACCAA TGTAGAGGAG CCGACTCTGC AAGAGAATCC AGTGAGTTCC 
GCACAAAGTT GTGATAACTA TGCAGGCTCA GATGCTGCGT CTGGTAGAGA GATGAGTGAT 
GGTGGAGAAG GGGTTCATGG GAAACAGGAT GGAAAACCAA CCAAAAAGCA TCACCGGAGG 
TCTTCCCGCA CCCGCTCTCG CCAGGAGAAG GCCAATAAAC CAAAGCTGAC TATTCTTAAT 
GTGAGCAATA CAGGTGACAA GATGGTGGAA TGCCAGTTGG AGACTCACAA CCATAAAATG 
GTGACTTTCA AATTTGACTT GGATGGCGAT GCACCAGATG AGATTGCCAC GTACATGGTG 
GAGAATGGCT TTATTCTGCA GATGGAGAAG GAGGTGTTTA TTGAGCAAAT GAAGGATGTT 
ATGGACAAAG CGGAAGACAT GTTGAGCGAA GACACTGAAG GGGAGAGAAG TTCAGACCAA 
GCGGCGAGTC CTCAGAAAGT GGATTCTAAT GGGAGAGAGA CGAGGGAGGA AAACACCCCA 
AATCAAATGA AGACTCCGGT CTATCAGCAA AACGTTCTAC ATACTGGGAA GCGATGGTTT 
ATTATATGTC CAGTGGCCGA AACCACCGGT TCCTCCCTGC CAGAAGTGCC CCAAACTCCC 
TTAAATACCC AGAAGCCGAC TGACGTTAAA GATCAGACCG CCTGCGAGGA TTCAGTATTT 
GCCACAAATG CGGATAAGTT GGACTTTGCG GTGCCGCCGG GCATACTGTG TGAGAGTGCC 
AGTGCCATTC CTGCCTCAAG GACACAATCT TCTTCTACAA TATCTTCCTC CGATCTGCAA 
ACCCAAACGG AACTGTCCTT TTCTGCTCTT CCTGAGTCTC TCTCCTTTCT CCAAGAAGAG 
GAACCGGTCC TGTTCAGCCC GCCGGCAGTT ACGCAGCCTC TAATGGACTC CATAAGCCAT 
GAGGCCGGAG GGAGCCTGGC GCCTGTATTC TCCGCAGCAC AAGAACCCGA GCAGAAACCC 
AGTCCTACAG ATTCAGTAAA TTCAACAGAA CCGCCACGTG CAGCCTTACC CCTCTCCGAC 
CACGAACGAC CGGCCGCTCA GCAAGAACGG ACTCTAGATC CACCCCCAGA GCGGAGTACA 
TTGTATGGAT CCAGTATTGG TTTCCCCGTT GTAAACTCCA TAAAACCAGA TGCCCCCTTG 
CCCATACCCC CTTGCCAGCC ACACCTTGAT TCTGAAATTT CCACCCCACT GAAACCAGTC 
CCAGTCCCCA CTCAGTCTCT GGTTTTACCA GTTGGCGGCA CATCTTGCCA ACAGCCCAGT 
ATTACCGAGT CGGACGGGGA AGGCCCCCCG AGGGTAGAAT TTACCGACGA TACCATTAAA 
AGCTTAGATG AAAAGCTCCG CACTCTCTTG TACCAGGAAT ACACCCCGGC CAACGTTTCT 
GCCGGAACCC CTGACAATTG TTCCGGAGCA GAAAGCGGAG CAGAGACAAC GTTGGGCAAC 
GGAGAAGAGA TTGCTGAACA CAGAAACCTC TCCCAGTCTG TCTCTGCGCC ACTAGAGAAA 
GCAACGGAAC TGGGCTGGAC TGTCGCAGAG AAAAGTTCTT GTGAGCTGTC AAACCAAAAG 
ATTCAGGCTG CACCTCTGAA CCATTCCCAG GGGTGGGATT CTGGCAAAGA AAGTGCAGTT 
GACTCTTCTT CCAAAAAGAG AGAGACCCCT GACAAGTCCT CCAAGACCAT CGGCAGGTTC 
TCGGTTGTGA GTGCAGAAGA TGATGCCCCA GTGACCGCTC CAAATTACAC GAGATTTTCA 
GCCCCGCCCG ATGTCTACCT GGATCAGGCT CCACCACAAC CAGCCAGGGC CTCTGTCCCT 
AGAGCTCACA CCTCTTCCCC TATTAACTCT CTGTTCTGTG GCCATGTTTC CAGTGACTCT 
GGGGATGATA CCCCTCACTC CAAGTCCAAA GAGGCTCAGC CGTCCACCCC AAGTAAGGGG 
GGCACCAATG ACTTCATGAA GAAGGCCGCT GCTTTCTTGC ATCGCTCTGG AAAGGCCAGT 
ACCCAAAGCC CCACCTCTCC CAACGGCCAA GCCATGAAAA TCCCTTCAAT AAACGTGACC 
TCTTTCCATT CGCAGTCGTC CTATATGAGC AGTGATAACG ACTCGGAGTT TGAAGATGCC 
GACATGAAGA AGGAACTGCA GAGCCTCCGG GAGAAGCACA TGAAGGAAAT AACAGAACTG 
CAATCGCAGC AGAAGAAGGA GATTGAGGCC CTTTACCTCC GGCTGGGGAA GCCCCTCCCA 
CCCAATGTGG GATTCCTGCA CGCAGCACCC CCCAGTGGCC GCCGGAGGAG GATCAGCAAA 
AATAAGCTGA AAACTGGGAA ACTGCTCAAT CCGCTGGTGC AGCAGCTGAA GAATTCGACT 
ACCAGTCCAA GCCGCATTTC CCCAGAATGC AATGGTTCCC CTGCTAAGGA TCCTGCAAAG 
TCTCTGGCAG ACTGCACCGC AGGAACCTCG CCAAGCCGTG CCGTACAAAC GCAGCAGCCT 
TGCTCTGTGA AAGTCACCAT GTCGTCCGAT AATATCTGCT CTGGGGTCAC CCGGGACGGT 
GTCGATGTGC AGAGCAATTC AAGCCAAGGC TGGACGGTTT ACCACCAAAC GTCTGAGAGA 
GTGACCTATA AATCTAGTAG CAAACCTCGT ACCAGATTCC TCAGTGGACC TGTGTCTCTG 
TCCATCTGGT CCACTCTGAA ACGCCTGTGT CTAGGCAAAG ATCACAGTAG TAGATCTTCG 
ACAAGCAGCC TCGCAACCGC CCCCGAGGCC TCGGCGCTGC CCCCGCTGAA CGCACAAATC 
CAGCCGCAGG CCAACAACAG CAACAACAAC AAAAAGGGGA CGTTTACGGA TGACCTTCAC 
AAATTGGTGG ATGAGTGGGC CTGCAAAACC GTCGGCGTGG CACAATTCAA ACCCTCATTA 
AACCAATTGA AGCAGTACCA GCAGCGGCAA GATCTGGAGC CCAAGTCTGT GCCCAGCGAC 
CTGTGTGACA TGAGCCGTGC CGTGCCAGAG CCCAGGAAGA GATACAGCAT TCCCATGGCT 
GCCCCTCCGG CTGTACCACT GGCTCCCCTC CTCTCTGCCG CTGTCCCAGC AACTGTTCCA 
GGAGCCGTGC AGCCGGGTAC CTTTGTCATG CCCTTTTGTC CGTACGCGGC ACCAATGTAC 
GGCATGCCCT GGGCAGGAGC GGCTGAGCTT GGGACTGCAG GAAATCAGAT CATGGCCCAA 
GTAGCCCCCA TAGGCAACCA AATATTCCCT GTGGCACTGC AGCACCCAGG TTTGCACCCT 
GCTGGATCCA ACATGCGGAT TACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk2 ( XM_031901289.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757149.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.

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CloneID	OXa57684
Clone ID Related Accession (Same CDS sequence)	XM_031901289.1
Accession Version	XM_031901289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGTCGTCCTA TATGAGCAGT GATAACGACT CGGAGTTTGA AGATGCCGAC 
ATGAAGAAGG AACTGCAGAG CCTCCGGGAG AAGCACATGA AGGAAATAAC AGAACTGCAA 
TCGCAGCAGA AGAAGGAGAT TGAGGCCCTT TACCTCCGGC TGGGGAAGCC CCTCCCACCC 
AATGTGGGAT TCCTGCACGC AGCACCCCCC AGTGGCCGCC GGAGGAGGAT CAGCAAAAAT 
AAGCTGAAAA CTGGGAAACT GCTCAATCCG CTGGTGCAGC AGCTGAAGAA TTCGACTACC 
AGTCCAAGCC GCATTTCCCC AGAATGCAAT GGTTCCCCTG CTAAGGATCC TGCAAAGTCT 
CTGGCAGACT GCACCGCAGG AACCTCGCCA AGCCGTGCCG TACAAACGCA GCAGCCTTGC 
TCTGTGAAAG TCACCATGTC GTCCGATAAT ATCTGCTCTG GGGTCACCCG GGACGGTGTC 
GATGTGCAGA GCAATTCAAG CCAAGGCTGG ACGGTTTACC ACCAAACGTC TGAGAGAGTG 
ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTCTGTCC 
ATCTGGTCCA CTCTGAAACG CCTGTGTCTA GGCAAAGATC ACAGTAGTAG ATCTTCGACA 
AGCAGCCTCG CAACCGCCCC CGAGGCCTCG GCGCTGCCCC CGCTGAACGC ACAAATCCAG 
CCGCAGGCCA ACAACAGCAA CAACAACAAA AAGGGGACGT TTACGGATGA CCTTCACAAA 
TTGGTGGATG AGTGGGCCTG CAAAACCGTC GGCGTGGCAC AATTCAAACC CTCATTAAAC 
CAATTGAAGC AGTACCAGCA GCGGCAAGAT CTGGAGCCCA AGTCTGTGCC CAGCGACCTG 
TGTGACATGA GCCGTGCCGT GCCAGAGCCC AGGAAGAGAT ACAGCATTCC CATGGCTGCC 
CCTCCGGCTG TACCACTGGC TCCCCTCCTC TCTGCCGCTG TCCCAGCAAC TGTTCCAGGA 
GCCGTGCAGC CGGGTACCTT TGTCATGCCC TTTTGTCCGT ACGCGGCACC AATGTACGGC 
ATGCCCTGGG CAGGAGCGGC TGAGCTTGGG ACTGCAGGAA ATCAGATCAT GGCCCAAGTA 
GCCCCCATAG GCAACCAAAT ATTCCCTGTG GCACTGCAGC ACCCAGGTTT GCACCCTGCT 
GGATCCAACA TGCGGATTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757149.1
CDS	427..6690
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTPGIQVATGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQPHPVVQQIPAPQGICPMQSATHPFPQYQAPLAQVAVPQLKPLHIPPVPAQQRPVPATQLPPYPLIPAVTPLAGLETLPGASLSDFPAVCVTPLPAPCHYFPPGIVVASVPLTPAIAPPLPLQAMNSQLVLSYETNIVPNVPVGTVPLLAAAGVPTLPTPQAVSQHPQVQQFQTSYPPLIQSDVPSQNVSHQATVPPPQTHHPSIPHPSDQASAGACNQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGWTVYHQTSERVTYKSSSKPRTRFLSGPVSLSIWSTLKRLCLGKDHSSRSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901289.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901289.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGTCGTCCTA TATGAGCAGT GATAACGACT CGGAGTTTGA AGATGCCGAC 
ATGAAGAAGG AACTGCAGAG CCTCCGGGAG AAGCACATGA AGGAAATAAC AGAACTGCAA 
TCGCAGCAGA AGAAGGAGAT TGAGGCCCTT TACCTCCGGC TGGGGAAGCC CCTCCCACCC 
AATGTGGGAT TCCTGCACGC AGCACCCCCC AGTGGCCGCC GGAGGAGGAT CAGCAAAAAT 
AAGCTGAAAA CTGGGAAACT GCTCAATCCG CTGGTGCAGC AGCTGAAGAA TTCGACTACC 
AGTCCAAGCC GCATTTCCCC AGAATGCAAT GGTTCCCCTG CTAAGGATCC TGCAAAGTCT 
CTGGCAGACT GCACCGCAGG AACCTCGCCA AGCCGTGCCG TACAAACGCA GCAGCCTTGC 
TCTGTGAAAG TCACCATGTC GTCCGATAAT ATCTGCTCTG GGGTCACCCG GGACGGTGTC 
GATGTGCAGA GCAATTCAAG CCAAGGCTGG ACGGTTTACC ACCAAACGTC TGAGAGAGTG 
ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTCTGTCC 
ATCTGGTCCA CTCTGAAACG CCTGTGTCTA GGCAAAGATC ACAGTAGTAG ATCTTCGACA 
AGCAGCCTCG CAACCGCCCC CGAGGCCTCG GCGCTGCCCC CGCTGAACGC ACAAATCCAG 
CCGCAGGCCA ACAACAGCAA CAACAACAAA AAGGGGACGT TTACGGATGA CCTTCACAAA 
TTGGTGGATG AGTGGGCCTG CAAAACCGTC GGCGTGGCAC AATTCAAACC CTCATTAAAC 
CAATTGAAGC AGTACCAGCA GCGGCAAGAT CTGGAGCCCA AGTCTGTGCC CAGCGACCTG 
TGTGACATGA GCCGTGCCGT GCCAGAGCCC AGGAAGAGAT ACAGCATTCC CATGGCTGCC 
CCTCCGGCTG TACCACTGGC TCCCCTCCTC TCTGCCGCTG TCCCAGCAAC TGTTCCAGGA 
GCCGTGCAGC CGGGTACCTT TGTCATGCCC TTTTGTCCGT ACGCGGCACC AATGTACGGC 
ATGCCCTGGG CAGGAGCGGC TGAGCTTGGG ACTGCAGGAA ATCAGATCAT GGCCCAAGTA 
GCCCCCATAG GCAACCAAAT ATTCCCTGTG GCACTGCAGC ACCCAGGTTT GCACCCTGCT 
GGATCCAACA TGCGGATTAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk2 ( XM_031901290.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757150.1 Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.

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CloneID	OXa57685
Clone ID Related Accession (Same CDS sequence)	XM_031901290.1
Accession Version	XM_031901290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	serine/threonine-protein kinase WNK2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGATC TTCGACAAGC 
AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG CTGCCCCCGC TGAACGCACA AATCCAGCCG 
CAGGCCAACA ACAGCAACAA CAACAAAAAG GGGACGTTTA CGGATGACCT TCACAAATTG 
GTGGATGAGT GGGCCTGCAA AACCGTCGGC GTGGCACAAT TCAAACCCTC ATTAAACCAA 
TTGAAGCAGT ACCAGCAGCG GCAAGATCTG GAGCCCAAGT CTGTGCCCAG CGACCTGTGT 
GACATGAGCC GTGCCGTGCC AGAGCCCAGG AAGAGATACA GCATTCCCAT GGCTGCCCCT 
CCGGCTGTAC CACTGGCTCC CCTCCTCTCT GCCGCTGTCC CAGCAACTGT TCCAGGAGCC 
GTGCAGCCGG GTACCTTTGT CATGCCCTTT TGTCCGTACG CGGCACCAAT GTACGGCATG 
CCCTGGGCAG GAGCGGCTGA GCTTGGGACT GCAGGAAATC AGATCATGGC CCAAGTAGCC 
CCCATAGGCA ACCAAATATT CCCTGTGGCA CTGCAGCACC CAGGTTTGCA CCCTGCTGGA 
TCCAACATGC GGATTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757150.1
CDS	426..6626
Translation	MEAGVGAPSCIGSLGTVEPNGLAQLPINIQPIAKGVDTAMLSSGAGAQAGPAAKVSKTVKRRFLRRTVVDSDQEEPSVLDLSLSGHHNKVIPISRARRAIAKRSQGVSDQGGGTEVLESKGSSSKGGSADGEPDQPEGLENKEKAETSHDVPKCRKEAPAEEEEENEEEADMKAVSTSLDGRFLKFDIEIGRGSFKTVYKGLDTETWVEVAWCELQDRKLSKLERQRFKEEAEMLKGLQHPNIVRFYDFWESCLKGKKYIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLMFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGVKPASFDKVADPEIKEIIGECICKNKEERYGIKDLLSHAFFAEDTGVRVELAEEDNGRKSSIALRLWVEDPKKLKVKPKDNGAIEFTFDLEKETPEDVAQEMVESGFFHESDVKIVGKSIRDRVALIQWRRERICPGENEALEQPPVVQPPLAPLAHSSHIAPHIPPEPEELDTDQHLYQPNLPTSITSVASDGTFDSGQGSTVYSDSQSSQQSVIYSSLPDSLPPAIQRVYSPPILEGQTVPHFGQYQPPPTPGIQVATGTLPGLGQPHYQDPGAQYPMMQANAIPSVQFAQLQPVLPGQPHPVVQQIPAPQGICPMQSATHPFPQYQAPLAQVAVPQLKPLHIPPVPAQQRPVPATQLPPYPLIPAVTPLAGLETLPGASLSDFPAVCVTPLPAPCHYFPPGIVVASVPLTPAIAPPLPLQAMNSQLVLSYETNIVPNVPVGTVPLLAAAGVPTLPTPQAVSQHPQVQQFQTSYPPLIQSDVPSQNVSHQATVPPPQTHHPSIPHPSDQASAGACNQVVLPQYPLDVGTAMGVMVPSTTMSPPVQFPAESAPLHPPQEHVTPVSYIHTDVPMTQQSTTLQSEGHQPETTQPPVHQSQTLYQAPRPSVETNVEEPTLQENPVSSAQSCDNYAGSDAASGREMSDGGEGVHGKQDGKPTKKHHRRSSRTRSRQEKANKPKLTILNVSNTGDKMVECQLETHNHKMVTFKFDLDGDAPDEIATYMVENGFILQMEKEVFIEQMKDVMDKAEDMLSEDTEGERSSDQAASPQKVDSNGRETREENTPNQMKTPVYQQNVLHTGKRWFIICPVAETTGSSLPEVPQTPLNTQKPTDVKDQTACEDSVFATNADKLDFAVPPGILCESASAIPASRTQSSSTISSSDLQTQTELSFSALPESLSFLQEEEPVLFSPPAVTQPLMDSISHEAGGSLAPVFSAAQEPEQKPSPTDSVNSTEPPRAALPLSDHERPAAQQERTLDPPPERSTLYGSSIGFPVVNSIKPDAPLPIPPCQPHLDSEISTPLKPVPVPTQSLVLPVGGTSCQQPSITESDGEGPPRVEFTDDTIKSLDEKLRTLLYQEYTPANVSAGTPDNCSGAESGAETTLGNGEEIAEHRNLSQSVSAPLEKATELGWTVAEKSSCELSNQKIQAAPLNHSQGWDSGKESAVDSSSKKRETPDKSSKTIGRFSVVSAEDDAPVTAPNYTRFSAPPDVYLDQAPPQPARASVPRAHTSSPINSLFCGHVSSDSGDDTPHSKSKEAQPSTPSKGGTNDFMKKAAAFLHRSGKASTQSPTSPNGQAMKIPSINVTSFHSQSSYMSSDNDSEFEDADMKKELQSLREKHMKEITELQSQQKKEIEALYLRLGKPLPPNVGFLHAAPPSGRRRRISKNKLKTGKLLNPLVQQLKNSTTSPSRISPECNGSPAKDPAKSLADCTAGTSPSRAVQTQQPCSVKVTMSSDNICSGVTRDGVDVQSNSSQGSSTSSLATAPEASALPPLNAQIQPQANNSNNNKKGTFTDDLHKLVDEWACKTVGVAQFKPSLNQLKQYQQRQDLEPKSVPSDLCDMSRAVPEPRKRYSIPMAAPPAVPLAPLLSAAVPATVPGAVQPGTFVMPFCPYAAPMYGMPWAGAAELGTAGNQIMAQVAPIGNQIFPVALQHPGLHPAGSNMRIT

Target ORF information:

RefSeq Version	XM_031901290.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901290.1

ORF Insert Sequence:

ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG 
CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG 
CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT 
TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC 
GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA 
GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG 
AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG 
GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC 
GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG 
GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA 
AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC 
CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC 
GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG 
GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC 
CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC 
ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC 
TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA 
CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG 
TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC 
GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC 
GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC 
TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA 
TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA 
GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA 
CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA 
ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT 
GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC 
CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT 
CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC 
CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT 
CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT 
CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT 
ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG 
ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT 
GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG 
TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA 
CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG 
CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT 
GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC 
TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC 
CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA 
AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC 
CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC 
ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA 
ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG 
GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT 
ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG 
GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA 
ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA 
ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA 
GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT 
GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC 
AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA 
AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG 
ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG 
ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT 
GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG 
GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG 
AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT 
ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA 
GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC 
TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC 
ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA 
TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC 
TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA 
ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA 
GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA 
GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA 
CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA 
AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC 
ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA 
TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT 
ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC 
ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA 
GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC 
TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT 
GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT 
GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC 
AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA 
AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA 
GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC 
CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG 
TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT 
CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC 
CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC 
TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG 
AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG 
CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC 
CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG 
CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT 
GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC 
GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT 
GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGATC TTCGACAAGC 
AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG CTGCCCCCGC TGAACGCACA AATCCAGCCG 
CAGGCCAACA ACAGCAACAA CAACAAAAAG GGGACGTTTA CGGATGACCT TCACAAATTG 
GTGGATGAGT GGGCCTGCAA AACCGTCGGC GTGGCACAAT TCAAACCCTC ATTAAACCAA 
TTGAAGCAGT ACCAGCAGCG GCAAGATCTG GAGCCCAAGT CTGTGCCCAG CGACCTGTGT 
GACATGAGCC GTGCCGTGCC AGAGCCCAGG AAGAGATACA GCATTCCCAT GGCTGCCCCT 
CCGGCTGTAC CACTGGCTCC CCTCCTCTCT GCCGCTGTCC CAGCAACTGT TCCAGGAGCC 
GTGCAGCCGG GTACCTTTGT CATGCCCTTT TGTCCGTACG CGGCACCAAT GTACGGCATG 
CCCTGGGCAG GAGCGGCTGA GCTTGGGACT GCAGGAAATC AGATCATGGC CCAAGTAGCC 
CCCATAGGCA ACCAAATATT CCCTGTGGCA CTGCAGCACC CAGGTTTGCA CCCTGCTGGA 
TCCAACATGC GGATTACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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wnk2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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