dock8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dock8
Entrez Gene ID	100488105
Full Name	dedicator of cytokinesis 8
General protein information	Preferred Names dedicator of cytokinesis 8 Names dedicator of cytokinesis protein 8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012952557.2	XP_012808011.1	dedicator of cytokinesis protein 8 isoform X2
XM_012952557.3	XP_012808011.1	dedicator of cytokinesis protein 8 isoform X2
XM_002935601.5	XP_002935647.2	dedicator of cytokinesis protein 8 isoform X1
XM_002935601.4	XP_002935647.2	dedicator of cytokinesis protein 8 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOCK8	NP_001178461.1
Danio rerio (zebrafish)	dock8	XP_001920107.4
Homo sapiens (human)	DOCK8	NP_982272.2
Pan troglodytes (chimpanzee)	DOCK8	XP_003312029.1
Xenopus tropicalis (tropical clawed frog)	dock8	XP_002935647.2
Canis lupus familiaris (dog)	DOCK8	XP_005615946.1
Gallus gallus (chicken)	DOCK8	XP_424926.4
	LOC698081	XP_002808151.1
Rattus norvegicus (Norway rat)	Dock8	NP_001032882.2
Mus musculus (house mouse)	Dock8	NP_083061.2

Gene Ontology

The following dock8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dock8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03963	XM_002935601.5 Latest version!	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa34066	XM_012952557.3 Latest version!	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa34066	XM_012952557.2	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa03963	XM_002935601.4	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dock8 ( XM_002935601.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935647.2 Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

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CloneID	OXa03963
Clone ID Related Accession (Same CDS sequence)	XM_002935601.5 , XM_002935601.4
Accession Version	XM_002935601.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935601.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGGTT 
ACAGGTCGCA ACTTCAATCG TATGTCCCAT TTTATTGAAG ACAGTAATGA TATGCAGCAT 
GTCATTGCGT GCACAAGGAC ATCTATAATA AAGCACTTTC ATGAAGAAAT GGCCCTTCAA 
ATGGTTGTCA GCACTGGAAT GGTTAGAGAA AGTGTCTTCA AGTATGCTTG GTTCTTCTTT 
GAACTGCTGG TAAAGAGCAT GGCCCTATAT GTTCACAGTG TTGACAAACA GGATGTCCCC 
AGGAGAAGTC GCTTCTCAGA CCGGTTTAAA GATGACATAA GCACCATTGT AAATGTTGTC 
ACTTCTGAGA TTGGATCTCT CTTAAGTAAA CCTCAAAAAG AGTCGGAACA AGCTGAGAAA 
ATCAACATAA GTCTAGCATT TTTCTTATAT GATTTACTAT CTCTGATGGA CAGAGGTTTT 
GTTTTTAACC TAATCAAGCA CTACTGTAAT CAGCTGACCT CTAAGCTGAA TTCTTTCCCA 
ACCTTGATTT CTATGAGACT GGAGTTCCTG AGGATCCTTT GTAGCCATGA GCATTATCTC 
AATCTAAACC TCTTCTTTGT AACTTCCTCC ACGCCTGCTT CTCCATGCCC TTCTATCTCT 
TCAGAGAACT CCAGCTCCTG CTCCAGTTTT CAGGATCAGA AAATTGCAAG CATGTTTGAC 
CTCTCACCAG ATTACCGGCA AAAGCATTTT CTAACTGGGC TTCTGTTTAC AGAGTTGGAT 
GCTGCCCTGG ACTCTGAGAT GGAAGGAATT GGCAAGGTTC AAAGGAAAGC TATAGGTGCA 
ATTTACAACC TGCTGAGTTC ACATGACCTG GACCCACGCT GCCAAAGAAT AGAGACAAAA 
CAAAAAATTG CTGATCTTTA CCTGCCTTTG GTCAGTACCA TTTTAGATGC ACTTCCTCAA 
CTACATGATT TTTCAGCTGT AGACACACGA AGTGGAAAAG CCAGAACCTC AGATGAAGAG 
TCAGAAACTC CAAGCACAAT TAACCCAAGT GTAGCAATGG CTGTTGCTGG AAACCAATTC 
AACATAAAAA CATCTACCAT ATCTTCTGTG CCAGTTAAAG TGTACTCAAC CCTAAGCCCT 
GAATCCAGCA GAATCCTGCT AACATGCTTC CTTTGGATCA TGAAGAATGC TGAGCAGAAT 
GTGATTCAGA AGTGGATCAC CTATATGCCT ATTCTGCAGC TAAACAGAAT TCTGGATCTG 
CTCTACATGT GCGTTTCCTG CTTTGAATAC AAAGGAAGGC AATCTTCAGA CAAAGTCAGC 
ACCTTGGTGC TTCAGAAATC AAGAGATGTT AAGGCACGCC TGGAAGAAGC ATTGCTTAGG 
GGTGAAGGCG CCAGGGGTGA AATGATGAGA CGCTGGAAGA CAGGGAATGA AAGATTTGCT 
GGACTGAATG AAAATTTACG GTGGAGAAAA GAACAGACAC AGTGGCGTCA AGCTAATGAG 
AGGTTAAATA TGACAAAAGC TGAAATTGAA CAAGAAGCCA TAATGTGTAG GAACCTGGCA 
ACAGAAGCAA ATGTAACTAT TTTGGATATA GTGGAAACTA TCATACAGAC CAGTTTGTCC 
ATTGATTGCA AAGATAATCT TCTGGGAGGA GTCCTTAAAG TTCTTGTACA TTCCTTGAGT 
ACGAATCAGA GCAACACCTT CCTTACTCAC TGTTTTTCAA CCATCCGTCT GCTAATTATT 
AAGTTTGCAG ATTTTCTGTT TGAAGAAGAA GTGGAGCAAT GCTCAGATCT GTGTCAAAAG 
GTCCTACACC ACTGTTGCAG TTCCTTGGAA AGCACTCGGA GCCAGGCCTG TGCCACTCTG 
TACCTCATCA TGAGATTCAG CTTTAGCAAT ATCAGTAACT TTGCCAGAGT GAAGATGCAG 
GTAACTATGT CACTGGCATC CTTGGTTGGA AAATCACAAG ACTTCAATGA AGAGTTCCTG 
CGCAAATCAT TAAGGACGAT TCTGGCCTAT GCAGAAGAGG ATGAGGAGAT GCAGCCTACA 
CTTTTCCCAG CTCAGGTGGA TGACCTTTTA AATAACCTGA ACAGCATTCT GTCAGACACA 
GTTAAGATGA GGGCTTTCCA GGAGGACCCA GAAATGCTTA TGGATTTGAT GTATAGAATT 
GCCAAGGGCT ATCAGACCTC CCCAGACCTT AGGCTGACCT GGCTGCAGAA TATGGCAGAG 
AAACATGTGA AGAGGAAATG CTTTACTGAA GCAGCCATGT GTTTGGTTCA TGCTGCTGCC 
CTAGTGGCAG AATATTTAGG CATGTTAGAG GACTGCAGCT ATCTGCCCGT AGGCAGTGTT 
AGCTTTCAGA ATATTTCCTC CAATGTAATT GAGGAATCCG CAGTTTCTGA TGACATTCTC 
ACTCCAGATG ATGATGGTGT GTGTTCTGGA AGATATTTTT CAGAAAATGG GTTGATCGGA 
CTCTTGGAGC AGGCTGCTGA ACTTTTTAAT ACAGCAGCAT TCTATGAAAC TGTAAATGAA 
GTTTACAAAA TTGTCATCCC AGTTCTTGAG GCGCATCGTG ATTTCCACAA GCTCGCATCA 
ACCCATAAAA AACTTCAGAA AGCTTTTGAC AATATTGCTG AAAAGGGTCA GAAAAGAATG 
TTTGGAACAT ACTTCCGTGT AGGATTCTAT GGGTCAAAGT TTGGAGATTT AGATGAGCAA 
GAGTATATTT ATAAGGAACC TACGATTACA AAACTTCCTG AGATTTCCCA TAGATTAGAG 
AGTTTTTATG GTCAGTGTTT CGGAGATGAT GCTGTAGAAG TTATTAAAGA CTCTGTTCCT 
GTGGACAAAT CGAAATTGGA TCCTAATAAG GCTTACATAC AGATTACCTT TGTGGAGCCC 
TATTTTGAAG ACTATGAAAA GAAGTACAGA GCCACCCACT TCGAGAAGAA CTACAACCTT 
CGTCGCTTTA TGTATACAAC TCCTTTTACT CTAGATGGGC GTCCCAGAGG AGAACTCAGC 
GAACAGTACA AGAGAAAAAC CATCTTAACC ACAATACACG CATTTCCTTA CATTAAAACA 
CGCATCAATG TCATCCAGAA AGAGGAGTTC GAACTCACTC CTATAGAGGT TGCCATAGAA 
GATATGCAGA AGAAAACACA TGAATTGGCT GATGCAACCA ACAAGGAGCC ACCTGATGCC 
AAGATGCTTC AAATGTTGCT TCAAGGCTCT GTAGGAGCCA CTGTAAACCA GGGACCACTA 
GAGGTAGCCC AAGTGTTTCT TGCTGAAATT CCAGCTGACC CTAAACTCTA TCGACATCAC 
AACAAGCTGA GATTGTGCTT TAAAGAGTTT ATTATGCGGT GTGGTGAGGC AGTCGAAAAG 
AACAAAAGGC TCATCACTGC TGACCAGAGA GAATATCAGC AGGAACTGAA GAAGAACTAT 
AACAAACTGA AAGAGAACCT GCGACCCATG ATCGAGCGCA AAATCCCAGA GCTATATAAG 
AATGTAGTCA AGACACGTGG TGATGTCCGA GGGCTGTACC ATAGAACAAG CTTCAGAAAA 
TACGACTCCC AGCTCTCTTC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935647.2
CDS	171..6440
Translation	MASLSSAERRAFALKLNRNSSAEIRKHLSLQYSGEFIPLRGLGTPTSSTLQLPPFYDVVDPVDFESFLMTHINNQDPEFRQELGDFFEDELDTVYKAKECRTVQPSLPEQGVELDPHVRDCISTYIRESLIVTRKSQENQVIGSFSRSGSRKDFPKTLQKQVFESDEAEPRPNEQNDDSPITLLIDESGQTALTSCDFDLRGLRPETRLKNLLQFSNVEDLERQNEEARKSNRQSDLFALSPPMDEEDTVEIRPVPDCPKEHMGYRIMVKLHTLKFEIDIEPLFASFALYDLKEKKKISENFHCDLNSEVYKGYLRNHIPNIATSSQAKSAVFSITYPSQDIFLVVKIEKVLQQGEISDCAEPYMVLKETESGKTKDKIEKLKSQAENFCQRLGKYRMPFAWAPVSLLNCINVMTLERDSPESENPNGRNPATDRRASWTAGRRFSDRCNSLEENTQLPFKPTTLTVNSFFKQEGDKLSDEDLFKHLADYKRSSLQRRIKSIPGLLKLEISPVCDGLSSCLSPELLPVKPEGRTRPIKEILEFPVKEVYVPHTIYRNILYVYPQRLNFANRLSSARNIAIKIQFMNGEDAKNAMPVIFGKSSGPEFLSEVTTAVTYHNKTPDFYEEVKIKLPAKLTNRHHLLFTFYHISCQSKQGACVETLLGYSWLPMLLNDRLQIGQYCLPVAMEKLPLNYSMHTPEKVPGQSPPIKWVEGHKGVFDLEVQAVSSVHTQDNHLEKFLTLCHALEGPVTFPIRVLDQKITESSLEHELKLSVVCLSSSRLEPLVLFLHLVLDKLFHLTVQPMVIGSQTANFSQYAFESVVSIVNSLHNSKDLSKDQHGRNCLLASYVYYVFRLPEVQRDTIIAAGNLPEMRYYTVGRTSAASVGSKLMQSRSLSSSNPDITGNQNNADEEVKNIMSSKVTGRNFNRMSHFIEDSNDMQHVIACTRTSIIKHFHEEMALQMVVSTGMVRESVFKYAWFFFELLVKSMALYVHSVDKQDVPRRSRFSDRFKDDISTIVNVVTSEIGSLLSKPQKESEQAEKINISLAFFLYDLLSLMDRGFVFNLIKHYCNQLTSKLNSFPTLISMRLEFLRILCSHEHYLNLNLFFVTSSTPASPCPSISSENSSSCSSFQDQKIASMFDLSPDYRQKHFLTGLLFTELDAALDSEMEGIGKVQRKAIGAIYNLLSSHDLDPRCQRIETKQKIADLYLPLVSTILDALPQLHDFSAVDTRSGKARTSDEESETPSTINPSVAMAVAGNQFNIKTSTISSVPVKVYSTLSPESSRILLTCFLWIMKNAEQNVIQKWITYMPILQLNRILDLLYMCVSCFEYKGRQSSDKVSTLVLQKSRDVKARLEEALLRGEGARGEMMRRWKTGNERFAGLNENLRWRKEQTQWRQANERLNMTKAEIEQEAIMCRNLATEANVTILDIVETIIQTSLSIDCKDNLLGGVLKVLVHSLSTNQSNTFLTHCFSTIRLLIIKFADFLFEEEVEQCSDLCQKVLHHCCSSLESTRSQACATLYLIMRFSFSNISNFARVKMQVTMSLASLVGKSQDFNEEFLRKSLRTILAYAEEDEEMQPTLFPAQVDDLLNNLNSILSDTVKMRAFQEDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHVKRKCFTEAAMCLVHAAALVAEYLGMLEDCSYLPVGSVSFQNISSNVIEESAVSDDILTPDDDGVCSGRYFSENGLIGLLEQAAELFNTAAFYETVNEVYKIVIPVLEAHRDFHKLASTHKKLQKAFDNIAEKGQKRMFGTYFRVGFYGSKFGDLDEQEYIYKEPTITKLPEISHRLESFYGQCFGDDAVEVIKDSVPVDKSKLDPNKAYIQITFVEPYFEDYEKKYRATHFEKNYNLRRFMYTTPFTLDGRPRGELSEQYKRKTILTTIHAFPYIKTRINVIQKEEFELTPIEVAIEDMQKKTHELADATNKEPPDAKMLQMLLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKNVVKTRGDVRGLYHRTSFRKYDSQLSSSG

Target ORF information:

RefSeq Version	XM_002935601.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935601.5

ORF Insert Sequence:

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGGTT 
ACAGGTCGCA ACTTCAATCG TATGTCCCAT TTTATTGAAG ACAGTAATGA TATGCAGCAT 
GTCATTGCGT GCACAAGGAC ATCTATAATA AAGCACTTTC ATGAAGAAAT GGCCCTTCAA 
ATGGTTGTCA GCACTGGAAT GGTTAGAGAA AGTGTCTTCA AGTATGCTTG GTTCTTCTTT 
GAACTGCTGG TAAAGAGCAT GGCCCTATAT GTTCACAGTG TTGACAAACA GGATGTCCCC 
AGGAGAAGTC GCTTCTCAGA CCGGTTTAAA GATGACATAA GCACCATTGT AAATGTTGTC 
ACTTCTGAGA TTGGATCTCT CTTAAGTAAA CCTCAAAAAG AGTCGGAACA AGCTGAGAAA 
ATCAACATAA GTCTAGCATT TTTCTTATAT GATTTACTAT CTCTGATGGA CAGAGGTTTT 
GTTTTTAACC TAATCAAGCA CTACTGTAAT CAGCTGACCT CTAAGCTGAA TTCTTTCCCA 
ACCTTGATTT CTATGAGACT GGAGTTCCTG AGGATCCTTT GTAGCCATGA GCATTATCTC 
AATCTAAACC TCTTCTTTGT AACTTCCTCC ACGCCTGCTT CTCCATGCCC TTCTATCTCT 
TCAGAGAACT CCAGCTCCTG CTCCAGTTTT CAGGATCAGA AAATTGCAAG CATGTTTGAC 
CTCTCACCAG ATTACCGGCA AAAGCATTTT CTAACTGGGC TTCTGTTTAC AGAGTTGGAT 
GCTGCCCTGG ACTCTGAGAT GGAAGGAATT GGCAAGGTTC AAAGGAAAGC TATAGGTGCA 
ATTTACAACC TGCTGAGTTC ACATGACCTG GACCCACGCT GCCAAAGAAT AGAGACAAAA 
CAAAAAATTG CTGATCTTTA CCTGCCTTTG GTCAGTACCA TTTTAGATGC ACTTCCTCAA 
CTACATGATT TTTCAGCTGT AGACACACGA AGTGGAAAAG CCAGAACCTC AGATGAAGAG 
TCAGAAACTC CAAGCACAAT TAACCCAAGT GTAGCAATGG CTGTTGCTGG AAACCAATTC 
AACATAAAAA CATCTACCAT ATCTTCTGTG CCAGTTAAAG TGTACTCAAC CCTAAGCCCT 
GAATCCAGCA GAATCCTGCT AACATGCTTC CTTTGGATCA TGAAGAATGC TGAGCAGAAT 
GTGATTCAGA AGTGGATCAC CTATATGCCT ATTCTGCAGC TAAACAGAAT TCTGGATCTG 
CTCTACATGT GCGTTTCCTG CTTTGAATAC AAAGGAAGGC AATCTTCAGA CAAAGTCAGC 
ACCTTGGTGC TTCAGAAATC AAGAGATGTT AAGGCACGCC TGGAAGAAGC ATTGCTTAGG 
GGTGAAGGCG CCAGGGGTGA AATGATGAGA CGCTGGAAGA CAGGGAATGA AAGATTTGCT 
GGACTGAATG AAAATTTACG GTGGAGAAAA GAACAGACAC AGTGGCGTCA AGCTAATGAG 
AGGTTAAATA TGACAAAAGC TGAAATTGAA CAAGAAGCCA TAATGTGTAG GAACCTGGCA 
ACAGAAGCAA ATGTAACTAT TTTGGATATA GTGGAAACTA TCATACAGAC CAGTTTGTCC 
ATTGATTGCA AAGATAATCT TCTGGGAGGA GTCCTTAAAG TTCTTGTACA TTCCTTGAGT 
ACGAATCAGA GCAACACCTT CCTTACTCAC TGTTTTTCAA CCATCCGTCT GCTAATTATT 
AAGTTTGCAG ATTTTCTGTT TGAAGAAGAA GTGGAGCAAT GCTCAGATCT GTGTCAAAAG 
GTCCTACACC ACTGTTGCAG TTCCTTGGAA AGCACTCGGA GCCAGGCCTG TGCCACTCTG 
TACCTCATCA TGAGATTCAG CTTTAGCAAT ATCAGTAACT TTGCCAGAGT GAAGATGCAG 
GTAACTATGT CACTGGCATC CTTGGTTGGA AAATCACAAG ACTTCAATGA AGAGTTCCTG 
CGCAAATCAT TAAGGACGAT TCTGGCCTAT GCAGAAGAGG ATGAGGAGAT GCAGCCTACA 
CTTTTCCCAG CTCAGGTGGA TGACCTTTTA AATAACCTGA ACAGCATTCT GTCAGACACA 
GTTAAGATGA GGGCTTTCCA GGAGGACCCA GAAATGCTTA TGGATTTGAT GTATAGAATT 
GCCAAGGGCT ATCAGACCTC CCCAGACCTT AGGCTGACCT GGCTGCAGAA TATGGCAGAG 
AAACATGTGA AGAGGAAATG CTTTACTGAA GCAGCCATGT GTTTGGTTCA TGCTGCTGCC 
CTAGTGGCAG AATATTTAGG CATGTTAGAG GACTGCAGCT ATCTGCCCGT AGGCAGTGTT 
AGCTTTCAGA ATATTTCCTC CAATGTAATT GAGGAATCCG CAGTTTCTGA TGACATTCTC 
ACTCCAGATG ATGATGGTGT GTGTTCTGGA AGATATTTTT CAGAAAATGG GTTGATCGGA 
CTCTTGGAGC AGGCTGCTGA ACTTTTTAAT ACAGCAGCAT TCTATGAAAC TGTAAATGAA 
GTTTACAAAA TTGTCATCCC AGTTCTTGAG GCGCATCGTG ATTTCCACAA GCTCGCATCA 
ACCCATAAAA AACTTCAGAA AGCTTTTGAC AATATTGCTG AAAAGGGTCA GAAAAGAATG 
TTTGGAACAT ACTTCCGTGT AGGATTCTAT GGGTCAAAGT TTGGAGATTT AGATGAGCAA 
GAGTATATTT ATAAGGAACC TACGATTACA AAACTTCCTG AGATTTCCCA TAGATTAGAG 
AGTTTTTATG GTCAGTGTTT CGGAGATGAT GCTGTAGAAG TTATTAAAGA CTCTGTTCCT 
GTGGACAAAT CGAAATTGGA TCCTAATAAG GCTTACATAC AGATTACCTT TGTGGAGCCC 
TATTTTGAAG ACTATGAAAA GAAGTACAGA GCCACCCACT TCGAGAAGAA CTACAACCTT 
CGTCGCTTTA TGTATACAAC TCCTTTTACT CTAGATGGGC GTCCCAGAGG AGAACTCAGC 
GAACAGTACA AGAGAAAAAC CATCTTAACC ACAATACACG CATTTCCTTA CATTAAAACA 
CGCATCAATG TCATCCAGAA AGAGGAGTTC GAACTCACTC CTATAGAGGT TGCCATAGAA 
GATATGCAGA AGAAAACACA TGAATTGGCT GATGCAACCA ACAAGGAGCC ACCTGATGCC 
AAGATGCTTC AAATGTTGCT TCAAGGCTCT GTAGGAGCCA CTGTAAACCA GGGACCACTA 
GAGGTAGCCC AAGTGTTTCT TGCTGAAATT CCAGCTGACC CTAAACTCTA TCGACATCAC 
AACAAGCTGA GATTGTGCTT TAAAGAGTTT ATTATGCGGT GTGGTGAGGC AGTCGAAAAG 
AACAAAAGGC TCATCACTGC TGACCAGAGA GAATATCAGC AGGAACTGAA GAAGAACTAT 
AACAAACTGA AAGAGAACCT GCGACCCATG ATCGAGCGCA AAATCCCAGA GCTATATAAG 
AATGTAGTCA AGACACGTGG TGATGTCCGA GGGCTGTACC ATAGAACAAG CTTCAGAAAA 
TACGACTCCC AGCTCTCTTC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_012952557.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808011.1 Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

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CloneID	OXa34066
Clone ID Related Accession (Same CDS sequence)	XM_012952557.2 , XM_012952557.3
Accession Version	XM_012952557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGCAC 
TTTCATGAAG AAATGGCCCT TCAAATGGTT GTCAGCACTG GAATGGTTAG AGAAAGTGTC 
TTCAAGTATG CTTGGTTCTT CTTTGAACTG CTGGTAAAGA GCATGGCCCT ATATGTTCAC 
AGTGTTGACA AACAGGATGT CCCCAGGAGA AGTCGCTTCT CAGACCGGTT TAAAGATGAC 
ATAAGCACCA TTGTAAATGT TGTCACTTCT GAGATTGGAT CTCTCTTAAG TAAACCTCAA 
AAAGAGTCGG AACAAGCTGA GAAAATCAAC ATAAGTCTAG CATTTTTCTT ATATGATTTA 
CTATCTCTGA TGGACAGAGG TTTTGTTTTT AACCTAATCA AGCACTACTG TAATCAGCTG 
ACCTCTAAGC TGAATTCTTT CCCAACCTTG ATTTCTATGA GACTGGAGTT CCTGAGGATC 
CTTTGTAGCC ATGAGCATTA TCTCAATCTA AACCTCTTCT TTGTAACTTC CTCCACGCCT 
GCTTCTCCAT GCCCTTCTAT CTCTTCAGAG AACTCCAGCT CCTGCTCCAG TTTTCAGGAT 
CAGAAAATTG CAAGCATGTT TGACCTCTCA CCAGATTACC GGCAAAAGCA TTTTCTAACT 
GGGCTTCTGT TTACAGAGTT GGATGCTGCC CTGGACTCTG AGATGGAAGG AATTGGCAAG 
GTTCAAAGGA AAGCTATAGG TGCAATTTAC AACCTGCTGA GTTCACATGA CCTGGACCCA 
CGCTGCCAAA GAATAGAGAC AAAACAAAAA ATTGCTGATC TTTACCTGCC TTTGGTCAGT 
ACCATTTTAG ATGCACTTCC TCAACTACAT GATTTTTCAG CTGTAGACAC ACGAAGTGGA 
AAAGCCAGAA CCTCAGATGA AGAGTCAGAA ACTCCAAGCA CAATTAACCC AAGTGTAGCA 
ATGGCTGTTG CTGGAAACCA ATTCAACATA AAAACATCTA CCATATCTTC TGTGCCAGTT 
AAAGTGTACT CAACCCTAAG CCCTGAATCC AGCAGAATCC TGCTAACATG CTTCCTTTGG 
ATCATGAAGA ATGCTGAGCA GAATGTGATT CAGAAGTGGA TCACCTATAT GCCTATTCTG 
CAGCTAAACA GAATTCTGGA TCTGCTCTAC ATGTGCGTTT CCTGCTTTGA ATACAAAGGA 
AGGCAATCTT CAGACAAAGT CAGCACCTTG GTGCTTCAGA AATCAAGAGA TGTTAAGGCA 
CGCCTGGAAG AAGCATTGCT TAGGGGTGAA GGCGCCAGGG GTGAAATGAT GAGACGCTGG 
AAGACAGGGA ATGAAAGATT TGCTGGACTG AATGAAAATT TACGGTGGAG AAAAGAACAG 
ACACAGTGGC GTCAAGCTAA TGAGAGGTTA AATATGACAA AAGCTGAAAT TGAACAAGAA 
GCCATAATGT GTAGGAACCT GGCAACAGAA GCAAATGTAA CTATTTTGGA TATAGTGGAA 
ACTATCATAC AGACCAGTTT GTCCATTGAT TGCAAAGATA ATCTTCTGGG AGGAGTCCTT 
AAAGTTCTTG TACATTCCTT GAGTACGAAT CAGAGCAACA CCTTCCTTAC TCACTGTTTT 
TCAACCATCC GTCTGCTAAT TATTAAGTTT GCAGATTTTC TGTTTGAAGA AGAAGTGGAG 
CAATGCTCAG ATCTGTGTCA AAAGGTCCTA CACCACTGTT GCAGTTCCTT GGAAAGCACT 
CGGAGCCAGG CCTGTGCCAC TCTGTACCTC ATCATGAGAT TCAGCTTTAG CAATATCAGT 
AACTTTGCCA GAGTGAAGAT GCAGGTAACT ATGTCACTGG CATCCTTGGT TGGAAAATCA 
CAAGACTTCA ATGAAGAGTT CCTGCGCAAA TCATTAAGGA CGATTCTGGC CTATGCAGAA 
GAGGATGAGG AGATGCAGCC TACACTTTTC CCAGCTCAGG TGGATGACCT TTTAAATAAC 
CTGAACAGCA TTCTGTCAGA CACAGTTAAG ATGAGGGCTT TCCAGGAGGA CCCAGAAATG 
CTTATGGATT TGATGTATAG AATTGCCAAG GGCTATCAGA CCTCCCCAGA CCTTAGGCTG 
ACCTGGCTGC AGAATATGGC AGAGAAACAT GTGAAGAGGA AATGCTTTAC TGAAGCAGCC 
ATGTGTTTGG TTCATGCTGC TGCCCTAGTG GCAGAATATT TAGGCATGTT AGAGGACTGC 
AGCTATCTGC CCGTAGGCAG TGTTAGCTTT CAGAATATTT CCTCCAATGT AATTGAGGAA 
TCCGCAGTTT CTGATGACAT TCTCACTCCA GATGATGATG GTGTGTGTTC TGGAAGATAT 
TTTTCAGAAA ATGGGTTGAT CGGACTCTTG GAGCAGGCTG CTGAACTTTT TAATACAGCA 
GCATTCTATG AAACTGTAAA TGAAGTTTAC AAAATTGTCA TCCCAGTTCT TGAGGCGCAT 
CGTGATTTCC ACAAGCTCGC ATCAACCCAT AAAAAACTTC AGAAAGCTTT TGACAATATT 
GCTGAAAAGG GTCAGAAAAG AATGTTTGGA ACATACTTCC GTGTAGGATT CTATGGGTCA 
AAGTTTGGAG ATTTAGATGA GCAAGAGTAT ATTTATAAGG AACCTACGAT TACAAAACTT 
CCTGAGATTT CCCATAGATT AGAGAGTTTT TATGGTCAGT GTTTCGGAGA TGATGCTGTA 
GAAGTTATTA AAGACTCTGT TCCTGTGGAC AAATCGAAAT TGGATCCTAA TAAGGCTTAC 
ATACAGATTA CCTTTGTGGA GCCCTATTTT GAAGACTATG AAAAGAAGTA CAGAGCCACC 
CACTTCGAGA AGAACTACAA CCTTCGTCGC TTTATGTATA CAACTCCTTT TACTCTAGAT 
GGGCGTCCCA GAGGAGAACT CAGCGAACAG TACAAGAGAA AAACCATCTT AACCACAATA 
CACGCATTTC CTTACATTAA AACACGCATC AATGTCATCC AGAAAGAGGA GTTCGAACTC 
ACTCCTATAG AGGTTGCCAT AGAAGATATG CAGAAGAAAA CACATGAATT GGCTGATGCA 
ACCAACAAGG AGCCACCTGA TGCCAAGATG CTTCAAATGT TGCTTCAAGG CTCTGTAGGA 
GCCACTGTAA ACCAGGGACC ACTAGAGGTA GCCCAAGTGT TTCTTGCTGA AATTCCAGCT 
GACCCTAAAC TCTATCGACA TCACAACAAG CTGAGATTGT GCTTTAAAGA GTTTATTATG 
CGGTGTGGTG AGGCAGTCGA AAAGAACAAA AGGCTCATCA CTGCTGACCA GAGAGAATAT 
CAGCAGGAAC TGAAGAAGAA CTATAACAAA CTGAAAGAGA ACCTGCGACC CATGATCGAG 
CGCAAAATCC CAGAGCTATA TAAGAATGTA GTCAAGACAC GTGGTGATGT CCGAGGGCTG 
TACCATAGAA CAAGCTTCAG AAAATACGAC TCCCAGCTCT CTTCCAGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808011.1
CDS	171..6344
Translation	MASLSSAERRAFALKLNRNSSAEIRKHLSLQYSGEFIPLRGLGTPTSSTLQLPPFYDVVDPVDFESFLMTHINNQDPEFRQELGDFFEDELDTVYKAKECRTVQPSLPEQGVELDPHVRDCISTYIRESLIVTRKSQENQVIGSFSRSGSRKDFPKTLQKQVFESDEAEPRPNEQNDDSPITLLIDESGQTALTSCDFDLRGLRPETRLKNLLQFSNVEDLERQNEEARKSNRQSDLFALSPPMDEEDTVEIRPVPDCPKEHMGYRIMVKLHTLKFEIDIEPLFASFALYDLKEKKKISENFHCDLNSEVYKGYLRNHIPNIATSSQAKSAVFSITYPSQDIFLVVKIEKVLQQGEISDCAEPYMVLKETESGKTKDKIEKLKSQAENFCQRLGKYRMPFAWAPVSLLNCINVMTLERDSPESENPNGRNPATDRRASWTAGRRFSDRCNSLEENTQLPFKPTTLTVNSFFKQEGDKLSDEDLFKHLADYKRSSLQRRIKSIPGLLKLEISPVCDGLSSCLSPELLPVKPEGRTRPIKEILEFPVKEVYVPHTIYRNILYVYPQRLNFANRLSSARNIAIKIQFMNGEDAKNAMPVIFGKSSGPEFLSEVTTAVTYHNKTPDFYEEVKIKLPAKLTNRHHLLFTFYHISCQSKQGACVETLLGYSWLPMLLNDRLQIGQYCLPVAMEKLPLNYSMHTPEKVPGQSPPIKWVEGHKGVFDLEVQAVSSVHTQDNHLEKFLTLCHALEGPVTFPIRVLDQKITESSLEHELKLSVVCLSSSRLEPLVLFLHLVLDKLFHLTVQPMVIGSQTANFSQYAFESVVSIVNSLHNSKDLSKDQHGRNCLLASYVYYVFRLPEVQRDTIIAAGNLPEMRYYTVGRTSAASVGSKLMQSRSLSSSNPDITGNQNNADEEVKNIMSSKHFHEEMALQMVVSTGMVRESVFKYAWFFFELLVKSMALYVHSVDKQDVPRRSRFSDRFKDDISTIVNVVTSEIGSLLSKPQKESEQAEKINISLAFFLYDLLSLMDRGFVFNLIKHYCNQLTSKLNSFPTLISMRLEFLRILCSHEHYLNLNLFFVTSSTPASPCPSISSENSSSCSSFQDQKIASMFDLSPDYRQKHFLTGLLFTELDAALDSEMEGIGKVQRKAIGAIYNLLSSHDLDPRCQRIETKQKIADLYLPLVSTILDALPQLHDFSAVDTRSGKARTSDEESETPSTINPSVAMAVAGNQFNIKTSTISSVPVKVYSTLSPESSRILLTCFLWIMKNAEQNVIQKWITYMPILQLNRILDLLYMCVSCFEYKGRQSSDKVSTLVLQKSRDVKARLEEALLRGEGARGEMMRRWKTGNERFAGLNENLRWRKEQTQWRQANERLNMTKAEIEQEAIMCRNLATEANVTILDIVETIIQTSLSIDCKDNLLGGVLKVLVHSLSTNQSNTFLTHCFSTIRLLIIKFADFLFEEEVEQCSDLCQKVLHHCCSSLESTRSQACATLYLIMRFSFSNISNFARVKMQVTMSLASLVGKSQDFNEEFLRKSLRTILAYAEEDEEMQPTLFPAQVDDLLNNLNSILSDTVKMRAFQEDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHVKRKCFTEAAMCLVHAAALVAEYLGMLEDCSYLPVGSVSFQNISSNVIEESAVSDDILTPDDDGVCSGRYFSENGLIGLLEQAAELFNTAAFYETVNEVYKIVIPVLEAHRDFHKLASTHKKLQKAFDNIAEKGQKRMFGTYFRVGFYGSKFGDLDEQEYIYKEPTITKLPEISHRLESFYGQCFGDDAVEVIKDSVPVDKSKLDPNKAYIQITFVEPYFEDYEKKYRATHFEKNYNLRRFMYTTPFTLDGRPRGELSEQYKRKTILTTIHAFPYIKTRINVIQKEEFELTPIEVAIEDMQKKTHELADATNKEPPDAKMLQMLLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKNVVKTRGDVRGLYHRTSFRKYDSQLSSSG

Target ORF information:

RefSeq Version	XM_012952557.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952557.3

ORF Insert Sequence:

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGCAC 
TTTCATGAAG AAATGGCCCT TCAAATGGTT GTCAGCACTG GAATGGTTAG AGAAAGTGTC 
TTCAAGTATG CTTGGTTCTT CTTTGAACTG CTGGTAAAGA GCATGGCCCT ATATGTTCAC 
AGTGTTGACA AACAGGATGT CCCCAGGAGA AGTCGCTTCT CAGACCGGTT TAAAGATGAC 
ATAAGCACCA TTGTAAATGT TGTCACTTCT GAGATTGGAT CTCTCTTAAG TAAACCTCAA 
AAAGAGTCGG AACAAGCTGA GAAAATCAAC ATAAGTCTAG CATTTTTCTT ATATGATTTA 
CTATCTCTGA TGGACAGAGG TTTTGTTTTT AACCTAATCA AGCACTACTG TAATCAGCTG 
ACCTCTAAGC TGAATTCTTT CCCAACCTTG ATTTCTATGA GACTGGAGTT CCTGAGGATC 
CTTTGTAGCC ATGAGCATTA TCTCAATCTA AACCTCTTCT TTGTAACTTC CTCCACGCCT 
GCTTCTCCAT GCCCTTCTAT CTCTTCAGAG AACTCCAGCT CCTGCTCCAG TTTTCAGGAT 
CAGAAAATTG CAAGCATGTT TGACCTCTCA CCAGATTACC GGCAAAAGCA TTTTCTAACT 
GGGCTTCTGT TTACAGAGTT GGATGCTGCC CTGGACTCTG AGATGGAAGG AATTGGCAAG 
GTTCAAAGGA AAGCTATAGG TGCAATTTAC AACCTGCTGA GTTCACATGA CCTGGACCCA 
CGCTGCCAAA GAATAGAGAC AAAACAAAAA ATTGCTGATC TTTACCTGCC TTTGGTCAGT 
ACCATTTTAG ATGCACTTCC TCAACTACAT GATTTTTCAG CTGTAGACAC ACGAAGTGGA 
AAAGCCAGAA CCTCAGATGA AGAGTCAGAA ACTCCAAGCA CAATTAACCC AAGTGTAGCA 
ATGGCTGTTG CTGGAAACCA ATTCAACATA AAAACATCTA CCATATCTTC TGTGCCAGTT 
AAAGTGTACT CAACCCTAAG CCCTGAATCC AGCAGAATCC TGCTAACATG CTTCCTTTGG 
ATCATGAAGA ATGCTGAGCA GAATGTGATT CAGAAGTGGA TCACCTATAT GCCTATTCTG 
CAGCTAAACA GAATTCTGGA TCTGCTCTAC ATGTGCGTTT CCTGCTTTGA ATACAAAGGA 
AGGCAATCTT CAGACAAAGT CAGCACCTTG GTGCTTCAGA AATCAAGAGA TGTTAAGGCA 
CGCCTGGAAG AAGCATTGCT TAGGGGTGAA GGCGCCAGGG GTGAAATGAT GAGACGCTGG 
AAGACAGGGA ATGAAAGATT TGCTGGACTG AATGAAAATT TACGGTGGAG AAAAGAACAG 
ACACAGTGGC GTCAAGCTAA TGAGAGGTTA AATATGACAA AAGCTGAAAT TGAACAAGAA 
GCCATAATGT GTAGGAACCT GGCAACAGAA GCAAATGTAA CTATTTTGGA TATAGTGGAA 
ACTATCATAC AGACCAGTTT GTCCATTGAT TGCAAAGATA ATCTTCTGGG AGGAGTCCTT 
AAAGTTCTTG TACATTCCTT GAGTACGAAT CAGAGCAACA CCTTCCTTAC TCACTGTTTT 
TCAACCATCC GTCTGCTAAT TATTAAGTTT GCAGATTTTC TGTTTGAAGA AGAAGTGGAG 
CAATGCTCAG ATCTGTGTCA AAAGGTCCTA CACCACTGTT GCAGTTCCTT GGAAAGCACT 
CGGAGCCAGG CCTGTGCCAC TCTGTACCTC ATCATGAGAT TCAGCTTTAG CAATATCAGT 
AACTTTGCCA GAGTGAAGAT GCAGGTAACT ATGTCACTGG CATCCTTGGT TGGAAAATCA 
CAAGACTTCA ATGAAGAGTT CCTGCGCAAA TCATTAAGGA CGATTCTGGC CTATGCAGAA 
GAGGATGAGG AGATGCAGCC TACACTTTTC CCAGCTCAGG TGGATGACCT TTTAAATAAC 
CTGAACAGCA TTCTGTCAGA CACAGTTAAG ATGAGGGCTT TCCAGGAGGA CCCAGAAATG 
CTTATGGATT TGATGTATAG AATTGCCAAG GGCTATCAGA CCTCCCCAGA CCTTAGGCTG 
ACCTGGCTGC AGAATATGGC AGAGAAACAT GTGAAGAGGA AATGCTTTAC TGAAGCAGCC 
ATGTGTTTGG TTCATGCTGC TGCCCTAGTG GCAGAATATT TAGGCATGTT AGAGGACTGC 
AGCTATCTGC CCGTAGGCAG TGTTAGCTTT CAGAATATTT CCTCCAATGT AATTGAGGAA 
TCCGCAGTTT CTGATGACAT TCTCACTCCA GATGATGATG GTGTGTGTTC TGGAAGATAT 
TTTTCAGAAA ATGGGTTGAT CGGACTCTTG GAGCAGGCTG CTGAACTTTT TAATACAGCA 
GCATTCTATG AAACTGTAAA TGAAGTTTAC AAAATTGTCA TCCCAGTTCT TGAGGCGCAT 
CGTGATTTCC ACAAGCTCGC ATCAACCCAT AAAAAACTTC AGAAAGCTTT TGACAATATT 
GCTGAAAAGG GTCAGAAAAG AATGTTTGGA ACATACTTCC GTGTAGGATT CTATGGGTCA 
AAGTTTGGAG ATTTAGATGA GCAAGAGTAT ATTTATAAGG AACCTACGAT TACAAAACTT 
CCTGAGATTT CCCATAGATT AGAGAGTTTT TATGGTCAGT GTTTCGGAGA TGATGCTGTA 
GAAGTTATTA AAGACTCTGT TCCTGTGGAC AAATCGAAAT TGGATCCTAA TAAGGCTTAC 
ATACAGATTA CCTTTGTGGA GCCCTATTTT GAAGACTATG AAAAGAAGTA CAGAGCCACC 
CACTTCGAGA AGAACTACAA CCTTCGTCGC TTTATGTATA CAACTCCTTT TACTCTAGAT 
GGGCGTCCCA GAGGAGAACT CAGCGAACAG TACAAGAGAA AAACCATCTT AACCACAATA 
CACGCATTTC CTTACATTAA AACACGCATC AATGTCATCC AGAAAGAGGA GTTCGAACTC 
ACTCCTATAG AGGTTGCCAT AGAAGATATG CAGAAGAAAA CACATGAATT GGCTGATGCA 
ACCAACAAGG AGCCACCTGA TGCCAAGATG CTTCAAATGT TGCTTCAAGG CTCTGTAGGA 
GCCACTGTAA ACCAGGGACC ACTAGAGGTA GCCCAAGTGT TTCTTGCTGA AATTCCAGCT 
GACCCTAAAC TCTATCGACA TCACAACAAG CTGAGATTGT GCTTTAAAGA GTTTATTATG 
CGGTGTGGTG AGGCAGTCGA AAAGAACAAA AGGCTCATCA CTGCTGACCA GAGAGAATAT 
CAGCAGGAAC TGAAGAAGAA CTATAACAAA CTGAAAGAGA ACCTGCGACC CATGATCGAG 
CGCAAAATCC CAGAGCTATA TAAGAATGTA GTCAAGACAC GTGGTGATGT CCGAGGGCTG 
TACCATAGAA CAAGCTTCAG AAAATACGAC TCCCAGCTCT CTTCCAGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_002935601.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935647.2 Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

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CloneID	OXa03963
Clone ID Related Accession (Same CDS sequence)	XM_002935601.5 , XM_002935601.4
Accession Version	XM_002935601.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935601.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGGTT 
ACAGGTCGCA ACTTCAATCG TATGTCCCAT TTTATTGAAG ACAGTAATGA TATGCAGCAT 
GTCATTGCGT GCACAAGGAC ATCTATAATA AAGCACTTTC ATGAAGAAAT GGCCCTTCAA 
ATGGTTGTCA GCACTGGAAT GGTTAGAGAA AGTGTCTTCA AGTATGCTTG GTTCTTCTTT 
GAACTGCTGG TAAAGAGCAT GGCCCTATAT GTTCACAGTG TTGACAAACA GGATGTCCCC 
AGGAGAAGTC GCTTCTCAGA CCGGTTTAAA GATGACATAA GCACCATTGT AAATGTTGTC 
ACTTCTGAGA TTGGATCTCT CTTAAGTAAA CCTCAAAAAG AGTCGGAACA AGCTGAGAAA 
ATCAACATAA GTCTAGCATT TTTCTTATAT GATTTACTAT CTCTGATGGA CAGAGGTTTT 
GTTTTTAACC TAATCAAGCA CTACTGTAAT CAGCTGACCT CTAAGCTGAA TTCTTTCCCA 
ACCTTGATTT CTATGAGACT GGAGTTCCTG AGGATCCTTT GTAGCCATGA GCATTATCTC 
AATCTAAACC TCTTCTTTGT AACTTCCTCC ACGCCTGCTT CTCCATGCCC TTCTATCTCT 
TCAGAGAACT CCAGCTCCTG CTCCAGTTTT CAGGATCAGA AAATTGCAAG CATGTTTGAC 
CTCTCACCAG ATTACCGGCA AAAGCATTTT CTAACTGGGC TTCTGTTTAC AGAGTTGGAT 
GCTGCCCTGG ACTCTGAGAT GGAAGGAATT GGCAAGGTTC AAAGGAAAGC TATAGGTGCA 
ATTTACAACC TGCTGAGTTC ACATGACCTG GACCCACGCT GCCAAAGAAT AGAGACAAAA 
CAAAAAATTG CTGATCTTTA CCTGCCTTTG GTCAGTACCA TTTTAGATGC ACTTCCTCAA 
CTACATGATT TTTCAGCTGT AGACACACGA AGTGGAAAAG CCAGAACCTC AGATGAAGAG 
TCAGAAACTC CAAGCACAAT TAACCCAAGT GTAGCAATGG CTGTTGCTGG AAACCAATTC 
AACATAAAAA CATCTACCAT ATCTTCTGTG CCAGTTAAAG TGTACTCAAC CCTAAGCCCT 
GAATCCAGCA GAATCCTGCT AACATGCTTC CTTTGGATCA TGAAGAATGC TGAGCAGAAT 
GTGATTCAGA AGTGGATCAC CTATATGCCT ATTCTGCAGC TAAACAGAAT TCTGGATCTG 
CTCTACATGT GCGTTTCCTG CTTTGAATAC AAAGGAAGGC AATCTTCAGA CAAAGTCAGC 
ACCTTGGTGC TTCAGAAATC AAGAGATGTT AAGGCACGCC TGGAAGAAGC ATTGCTTAGG 
GGTGAAGGCG CCAGGGGTGA AATGATGAGA CGCTGGAAGA CAGGGAATGA AAGATTTGCT 
GGACTGAATG AAAATTTACG GTGGAGAAAA GAACAGACAC AGTGGCGTCA AGCTAATGAG 
AGGTTAAATA TGACAAAAGC TGAAATTGAA CAAGAAGCCA TAATGTGTAG GAACCTGGCA 
ACAGAAGCAA ATGTAACTAT TTTGGATATA GTGGAAACTA TCATACAGAC CAGTTTGTCC 
ATTGATTGCA AAGATAATCT TCTGGGAGGA GTCCTTAAAG TTCTTGTACA TTCCTTGAGT 
ACGAATCAGA GCAACACCTT CCTTACTCAC TGTTTTTCAA CCATCCGTCT GCTAATTATT 
AAGTTTGCAG ATTTTCTGTT TGAAGAAGAA GTGGAGCAAT GCTCAGATCT GTGTCAAAAG 
GTCCTACACC ACTGTTGCAG TTCCTTGGAA AGCACTCGGA GCCAGGCCTG TGCCACTCTG 
TACCTCATCA TGAGATTCAG CTTTAGCAAT ATCAGTAACT TTGCCAGAGT GAAGATGCAG 
GTAACTATGT CACTGGCATC CTTGGTTGGA AAATCACAAG ACTTCAATGA AGAGTTCCTG 
CGCAAATCAT TAAGGACGAT TCTGGCCTAT GCAGAAGAGG ATGAGGAGAT GCAGCCTACA 
CTTTTCCCAG CTCAGGTGGA TGACCTTTTA AATAACCTGA ACAGCATTCT GTCAGACACA 
GTTAAGATGA GGGCTTTCCA GGAGGACCCA GAAATGCTTA TGGATTTGAT GTATAGAATT 
GCCAAGGGCT ATCAGACCTC CCCAGACCTT AGGCTGACCT GGCTGCAGAA TATGGCAGAG 
AAACATGTGA AGAGGAAATG CTTTACTGAA GCAGCCATGT GTTTGGTTCA TGCTGCTGCC 
CTAGTGGCAG AATATTTAGG CATGTTAGAG GACTGCAGCT ATCTGCCCGT AGGCAGTGTT 
AGCTTTCAGA ATATTTCCTC CAATGTAATT GAGGAATCCG CAGTTTCTGA TGACATTCTC 
ACTCCAGATG ATGATGGTGT GTGTTCTGGA AGATATTTTT CAGAAAATGG GTTGATCGGA 
CTCTTGGAGC AGGCTGCTGA ACTTTTTAAT ACAGCAGCAT TCTATGAAAC TGTAAATGAA 
GTTTACAAAA TTGTCATCCC AGTTCTTGAG GCGCATCGTG ATTTCCACAA GCTCGCATCA 
ACCCATAAAA AACTTCAGAA AGCTTTTGAC AATATTGCTG AAAAGGGTCA GAAAAGAATG 
TTTGGAACAT ACTTCCGTGT AGGATTCTAT GGGTCAAAGT TTGGAGATTT AGATGAGCAA 
GAGTATATTT ATAAGGAACC TACGATTACA AAACTTCCTG AGATTTCCCA TAGATTAGAG 
AGTTTTTATG GTCAGTGTTT CGGAGATGAT GCTGTAGAAG TTATTAAAGA CTCTGTTCCT 
GTGGACAAAT CGAAATTGGA TCCTAATAAG GCTTACATAC AGATTACCTT TGTGGAGCCC 
TATTTTGAAG ACTATGAAAA GAAGTACAGA GCCACCCACT TCGAGAAGAA CTACAACCTT 
CGTCGCTTTA TGTATACAAC TCCTTTTACT CTAGATGGGC GTCCCAGAGG AGAACTCAGC 
GAACAGTACA AGAGAAAAAC CATCTTAACC ACAATACACG CATTTCCTTA CATTAAAACA 
CGCATCAATG TCATCCAGAA AGAGGAGTTC GAACTCACTC CTATAGAGGT TGCCATAGAA 
GATATGCAGA AGAAAACACA TGAATTGGCT GATGCAACCA ACAAGGAGCC ACCTGATGCC 
AAGATGCTTC AAATGTTGCT TCAAGGCTCT GTAGGAGCCA CTGTAAACCA GGGACCACTA 
GAGGTAGCCC AAGTGTTTCT TGCTGAAATT CCAGCTGACC CTAAACTCTA TCGACATCAC 
AACAAGCTGA GATTGTGCTT TAAAGAGTTT ATTATGCGGT GTGGTGAGGC AGTCGAAAAG 
AACAAAAGGC TCATCACTGC TGACCAGAGA GAATATCAGC AGGAACTGAA GAAGAACTAT 
AACAAACTGA AAGAGAACCT GCGACCCATG ATCGAGCGCA AAATCCCAGA GCTATATAAG 
AATGTAGTCA AGACACGTGG TGATGTCCGA GGGCTGTACC ATAGAACAAG CTTCAGAAAA 
TACGACTCCC AGCTCTCTTC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935647.2
CDS	195..6464
Translation	MASLSSAERRAFALKLNRNSSAEIRKHLSLQYSGEFIPLRGLGTPTSSTLQLPPFYDVVDPVDFESFLMTHINNQDPEFRQELGDFFEDELDTVYKAKECRTVQPSLPEQGVELDPHVRDCISTYIRESLIVTRKSQENQVIGSFSRSGSRKDFPKTLQKQVFESDEAEPRPNEQNDDSPITLLIDESGQTALTSCDFDLRGLRPETRLKNLLQFSNVEDLERQNEEARKSNRQSDLFALSPPMDEEDTVEIRPVPDCPKEHMGYRIMVKLHTLKFEIDIEPLFASFALYDLKEKKKISENFHCDLNSEVYKGYLRNHIPNIATSSQAKSAVFSITYPSQDIFLVVKIEKVLQQGEISDCAEPYMVLKETESGKTKDKIEKLKSQAENFCQRLGKYRMPFAWAPVSLLNCINVMTLERDSPESENPNGRNPATDRRASWTAGRRFSDRCNSLEENTQLPFKPTTLTVNSFFKQEGDKLSDEDLFKHLADYKRSSLQRRIKSIPGLLKLEISPVCDGLSSCLSPELLPVKPEGRTRPIKEILEFPVKEVYVPHTIYRNILYVYPQRLNFANRLSSARNIAIKIQFMNGEDAKNAMPVIFGKSSGPEFLSEVTTAVTYHNKTPDFYEEVKIKLPAKLTNRHHLLFTFYHISCQSKQGACVETLLGYSWLPMLLNDRLQIGQYCLPVAMEKLPLNYSMHTPEKVPGQSPPIKWVEGHKGVFDLEVQAVSSVHTQDNHLEKFLTLCHALEGPVTFPIRVLDQKITESSLEHELKLSVVCLSSSRLEPLVLFLHLVLDKLFHLTVQPMVIGSQTANFSQYAFESVVSIVNSLHNSKDLSKDQHGRNCLLASYVYYVFRLPEVQRDTIIAAGNLPEMRYYTVGRTSAASVGSKLMQSRSLSSSNPDITGNQNNADEEVKNIMSSKVTGRNFNRMSHFIEDSNDMQHVIACTRTSIIKHFHEEMALQMVVSTGMVRESVFKYAWFFFELLVKSMALYVHSVDKQDVPRRSRFSDRFKDDISTIVNVVTSEIGSLLSKPQKESEQAEKINISLAFFLYDLLSLMDRGFVFNLIKHYCNQLTSKLNSFPTLISMRLEFLRILCSHEHYLNLNLFFVTSSTPASPCPSISSENSSSCSSFQDQKIASMFDLSPDYRQKHFLTGLLFTELDAALDSEMEGIGKVQRKAIGAIYNLLSSHDLDPRCQRIETKQKIADLYLPLVSTILDALPQLHDFSAVDTRSGKARTSDEESETPSTINPSVAMAVAGNQFNIKTSTISSVPVKVYSTLSPESSRILLTCFLWIMKNAEQNVIQKWITYMPILQLNRILDLLYMCVSCFEYKGRQSSDKVSTLVLQKSRDVKARLEEALLRGEGARGEMMRRWKTGNERFAGLNENLRWRKEQTQWRQANERLNMTKAEIEQEAIMCRNLATEANVTILDIVETIIQTSLSIDCKDNLLGGVLKVLVHSLSTNQSNTFLTHCFSTIRLLIIKFADFLFEEEVEQCSDLCQKVLHHCCSSLESTRSQACATLYLIMRFSFSNISNFARVKMQVTMSLASLVGKSQDFNEEFLRKSLRTILAYAEEDEEMQPTLFPAQVDDLLNNLNSILSDTVKMRAFQEDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHVKRKCFTEAAMCLVHAAALVAEYLGMLEDCSYLPVGSVSFQNISSNVIEESAVSDDILTPDDDGVCSGRYFSENGLIGLLEQAAELFNTAAFYETVNEVYKIVIPVLEAHRDFHKLASTHKKLQKAFDNIAEKGQKRMFGTYFRVGFYGSKFGDLDEQEYIYKEPTITKLPEISHRLESFYGQCFGDDAVEVIKDSVPVDKSKLDPNKAYIQITFVEPYFEDYEKKYRATHFEKNYNLRRFMYTTPFTLDGRPRGELSEQYKRKTILTTIHAFPYIKTRINVIQKEEFELTPIEVAIEDMQKKTHELADATNKEPPDAKMLQMLLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKNVVKTRGDVRGLYHRTSFRKYDSQLSSSG

Target ORF information:

RefSeq Version	XM_002935601.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935601.4

ORF Insert Sequence:

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGGTT 
ACAGGTCGCA ACTTCAATCG TATGTCCCAT TTTATTGAAG ACAGTAATGA TATGCAGCAT 
GTCATTGCGT GCACAAGGAC ATCTATAATA AAGCACTTTC ATGAAGAAAT GGCCCTTCAA 
ATGGTTGTCA GCACTGGAAT GGTTAGAGAA AGTGTCTTCA AGTATGCTTG GTTCTTCTTT 
GAACTGCTGG TAAAGAGCAT GGCCCTATAT GTTCACAGTG TTGACAAACA GGATGTCCCC 
AGGAGAAGTC GCTTCTCAGA CCGGTTTAAA GATGACATAA GCACCATTGT AAATGTTGTC 
ACTTCTGAGA TTGGATCTCT CTTAAGTAAA CCTCAAAAAG AGTCGGAACA AGCTGAGAAA 
ATCAACATAA GTCTAGCATT TTTCTTATAT GATTTACTAT CTCTGATGGA CAGAGGTTTT 
GTTTTTAACC TAATCAAGCA CTACTGTAAT CAGCTGACCT CTAAGCTGAA TTCTTTCCCA 
ACCTTGATTT CTATGAGACT GGAGTTCCTG AGGATCCTTT GTAGCCATGA GCATTATCTC 
AATCTAAACC TCTTCTTTGT AACTTCCTCC ACGCCTGCTT CTCCATGCCC TTCTATCTCT 
TCAGAGAACT CCAGCTCCTG CTCCAGTTTT CAGGATCAGA AAATTGCAAG CATGTTTGAC 
CTCTCACCAG ATTACCGGCA AAAGCATTTT CTAACTGGGC TTCTGTTTAC AGAGTTGGAT 
GCTGCCCTGG ACTCTGAGAT GGAAGGAATT GGCAAGGTTC AAAGGAAAGC TATAGGTGCA 
ATTTACAACC TGCTGAGTTC ACATGACCTG GACCCACGCT GCCAAAGAAT AGAGACAAAA 
CAAAAAATTG CTGATCTTTA CCTGCCTTTG GTCAGTACCA TTTTAGATGC ACTTCCTCAA 
CTACATGATT TTTCAGCTGT AGACACACGA AGTGGAAAAG CCAGAACCTC AGATGAAGAG 
TCAGAAACTC CAAGCACAAT TAACCCAAGT GTAGCAATGG CTGTTGCTGG AAACCAATTC 
AACATAAAAA CATCTACCAT ATCTTCTGTG CCAGTTAAAG TGTACTCAAC CCTAAGCCCT 
GAATCCAGCA GAATCCTGCT AACATGCTTC CTTTGGATCA TGAAGAATGC TGAGCAGAAT 
GTGATTCAGA AGTGGATCAC CTATATGCCT ATTCTGCAGC TAAACAGAAT TCTGGATCTG 
CTCTACATGT GCGTTTCCTG CTTTGAATAC AAAGGAAGGC AATCTTCAGA CAAAGTCAGC 
ACCTTGGTGC TTCAGAAATC AAGAGATGTT AAGGCACGCC TGGAAGAAGC ATTGCTTAGG 
GGTGAAGGCG CCAGGGGTGA AATGATGAGA CGCTGGAAGA CAGGGAATGA AAGATTTGCT 
GGACTGAATG AAAATTTACG GTGGAGAAAA GAACAGACAC AGTGGCGTCA AGCTAATGAG 
AGGTTAAATA TGACAAAAGC TGAAATTGAA CAAGAAGCCA TAATGTGTAG GAACCTGGCA 
ACAGAAGCAA ATGTAACTAT TTTGGATATA GTGGAAACTA TCATACAGAC CAGTTTGTCC 
ATTGATTGCA AAGATAATCT TCTGGGAGGA GTCCTTAAAG TTCTTGTACA TTCCTTGAGT 
ACGAATCAGA GCAACACCTT CCTTACTCAC TGTTTTTCAA CCATCCGTCT GCTAATTATT 
AAGTTTGCAG ATTTTCTGTT TGAAGAAGAA GTGGAGCAAT GCTCAGATCT GTGTCAAAAG 
GTCCTACACC ACTGTTGCAG TTCCTTGGAA AGCACTCGGA GCCAGGCCTG TGCCACTCTG 
TACCTCATCA TGAGATTCAG CTTTAGCAAT ATCAGTAACT TTGCCAGAGT GAAGATGCAG 
GTAACTATGT CACTGGCATC CTTGGTTGGA AAATCACAAG ACTTCAATGA AGAGTTCCTG 
CGCAAATCAT TAAGGACGAT TCTGGCCTAT GCAGAAGAGG ATGAGGAGAT GCAGCCTACA 
CTTTTCCCAG CTCAGGTGGA TGACCTTTTA AATAACCTGA ACAGCATTCT GTCAGACACA 
GTTAAGATGA GGGCTTTCCA GGAGGACCCA GAAATGCTTA TGGATTTGAT GTATAGAATT 
GCCAAGGGCT ATCAGACCTC CCCAGACCTT AGGCTGACCT GGCTGCAGAA TATGGCAGAG 
AAACATGTGA AGAGGAAATG CTTTACTGAA GCAGCCATGT GTTTGGTTCA TGCTGCTGCC 
CTAGTGGCAG AATATTTAGG CATGTTAGAG GACTGCAGCT ATCTGCCCGT AGGCAGTGTT 
AGCTTTCAGA ATATTTCCTC CAATGTAATT GAGGAATCCG CAGTTTCTGA TGACATTCTC 
ACTCCAGATG ATGATGGTGT GTGTTCTGGA AGATATTTTT CAGAAAATGG GTTGATCGGA 
CTCTTGGAGC AGGCTGCTGA ACTTTTTAAT ACAGCAGCAT TCTATGAAAC TGTAAATGAA 
GTTTACAAAA TTGTCATCCC AGTTCTTGAG GCGCATCGTG ATTTCCACAA GCTCGCATCA 
ACCCATAAAA AACTTCAGAA AGCTTTTGAC AATATTGCTG AAAAGGGTCA GAAAAGAATG 
TTTGGAACAT ACTTCCGTGT AGGATTCTAT GGGTCAAAGT TTGGAGATTT AGATGAGCAA 
GAGTATATTT ATAAGGAACC TACGATTACA AAACTTCCTG AGATTTCCCA TAGATTAGAG 
AGTTTTTATG GTCAGTGTTT CGGAGATGAT GCTGTAGAAG TTATTAAAGA CTCTGTTCCT 
GTGGACAAAT CGAAATTGGA TCCTAATAAG GCTTACATAC AGATTACCTT TGTGGAGCCC 
TATTTTGAAG ACTATGAAAA GAAGTACAGA GCCACCCACT TCGAGAAGAA CTACAACCTT 
CGTCGCTTTA TGTATACAAC TCCTTTTACT CTAGATGGGC GTCCCAGAGG AGAACTCAGC 
GAACAGTACA AGAGAAAAAC CATCTTAACC ACAATACACG CATTTCCTTA CATTAAAACA 
CGCATCAATG TCATCCAGAA AGAGGAGTTC GAACTCACTC CTATAGAGGT TGCCATAGAA 
GATATGCAGA AGAAAACACA TGAATTGGCT GATGCAACCA ACAAGGAGCC ACCTGATGCC 
AAGATGCTTC AAATGTTGCT TCAAGGCTCT GTAGGAGCCA CTGTAAACCA GGGACCACTA 
GAGGTAGCCC AAGTGTTTCT TGCTGAAATT CCAGCTGACC CTAAACTCTA TCGACATCAC 
AACAAGCTGA GATTGTGCTT TAAAGAGTTT ATTATGCGGT GTGGTGAGGC AGTCGAAAAG 
AACAAAAGGC TCATCACTGC TGACCAGAGA GAATATCAGC AGGAACTGAA GAAGAACTAT 
AACAAACTGA AAGAGAACCT GCGACCCATG ATCGAGCGCA AAATCCCAGA GCTATATAAG 
AATGTAGTCA AGACACGTGG TGATGTCCGA GGGCTGTACC ATAGAACAAG CTTCAGAAAA 
TACGACTCCC AGCTCTCTTC CAGTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_012952557.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808011.1 Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

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CloneID	OXa34066
Clone ID Related Accession (Same CDS sequence)	XM_012952557.2 , XM_012952557.3
Accession Version	XM_012952557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGCAC 
TTTCATGAAG AAATGGCCCT TCAAATGGTT GTCAGCACTG GAATGGTTAG AGAAAGTGTC 
TTCAAGTATG CTTGGTTCTT CTTTGAACTG CTGGTAAAGA GCATGGCCCT ATATGTTCAC 
AGTGTTGACA AACAGGATGT CCCCAGGAGA AGTCGCTTCT CAGACCGGTT TAAAGATGAC 
ATAAGCACCA TTGTAAATGT TGTCACTTCT GAGATTGGAT CTCTCTTAAG TAAACCTCAA 
AAAGAGTCGG AACAAGCTGA GAAAATCAAC ATAAGTCTAG CATTTTTCTT ATATGATTTA 
CTATCTCTGA TGGACAGAGG TTTTGTTTTT AACCTAATCA AGCACTACTG TAATCAGCTG 
ACCTCTAAGC TGAATTCTTT CCCAACCTTG ATTTCTATGA GACTGGAGTT CCTGAGGATC 
CTTTGTAGCC ATGAGCATTA TCTCAATCTA AACCTCTTCT TTGTAACTTC CTCCACGCCT 
GCTTCTCCAT GCCCTTCTAT CTCTTCAGAG AACTCCAGCT CCTGCTCCAG TTTTCAGGAT 
CAGAAAATTG CAAGCATGTT TGACCTCTCA CCAGATTACC GGCAAAAGCA TTTTCTAACT 
GGGCTTCTGT TTACAGAGTT GGATGCTGCC CTGGACTCTG AGATGGAAGG AATTGGCAAG 
GTTCAAAGGA AAGCTATAGG TGCAATTTAC AACCTGCTGA GTTCACATGA CCTGGACCCA 
CGCTGCCAAA GAATAGAGAC AAAACAAAAA ATTGCTGATC TTTACCTGCC TTTGGTCAGT 
ACCATTTTAG ATGCACTTCC TCAACTACAT GATTTTTCAG CTGTAGACAC ACGAAGTGGA 
AAAGCCAGAA CCTCAGATGA AGAGTCAGAA ACTCCAAGCA CAATTAACCC AAGTGTAGCA 
ATGGCTGTTG CTGGAAACCA ATTCAACATA AAAACATCTA CCATATCTTC TGTGCCAGTT 
AAAGTGTACT CAACCCTAAG CCCTGAATCC AGCAGAATCC TGCTAACATG CTTCCTTTGG 
ATCATGAAGA ATGCTGAGCA GAATGTGATT CAGAAGTGGA TCACCTATAT GCCTATTCTG 
CAGCTAAACA GAATTCTGGA TCTGCTCTAC ATGTGCGTTT CCTGCTTTGA ATACAAAGGA 
AGGCAATCTT CAGACAAAGT CAGCACCTTG GTGCTTCAGA AATCAAGAGA TGTTAAGGCA 
CGCCTGGAAG AAGCATTGCT TAGGGGTGAA GGCGCCAGGG GTGAAATGAT GAGACGCTGG 
AAGACAGGGA ATGAAAGATT TGCTGGACTG AATGAAAATT TACGGTGGAG AAAAGAACAG 
ACACAGTGGC GTCAAGCTAA TGAGAGGTTA AATATGACAA AAGCTGAAAT TGAACAAGAA 
GCCATAATGT GTAGGAACCT GGCAACAGAA GCAAATGTAA CTATTTTGGA TATAGTGGAA 
ACTATCATAC AGACCAGTTT GTCCATTGAT TGCAAAGATA ATCTTCTGGG AGGAGTCCTT 
AAAGTTCTTG TACATTCCTT GAGTACGAAT CAGAGCAACA CCTTCCTTAC TCACTGTTTT 
TCAACCATCC GTCTGCTAAT TATTAAGTTT GCAGATTTTC TGTTTGAAGA AGAAGTGGAG 
CAATGCTCAG ATCTGTGTCA AAAGGTCCTA CACCACTGTT GCAGTTCCTT GGAAAGCACT 
CGGAGCCAGG CCTGTGCCAC TCTGTACCTC ATCATGAGAT TCAGCTTTAG CAATATCAGT 
AACTTTGCCA GAGTGAAGAT GCAGGTAACT ATGTCACTGG CATCCTTGGT TGGAAAATCA 
CAAGACTTCA ATGAAGAGTT CCTGCGCAAA TCATTAAGGA CGATTCTGGC CTATGCAGAA 
GAGGATGAGG AGATGCAGCC TACACTTTTC CCAGCTCAGG TGGATGACCT TTTAAATAAC 
CTGAACAGCA TTCTGTCAGA CACAGTTAAG ATGAGGGCTT TCCAGGAGGA CCCAGAAATG 
CTTATGGATT TGATGTATAG AATTGCCAAG GGCTATCAGA CCTCCCCAGA CCTTAGGCTG 
ACCTGGCTGC AGAATATGGC AGAGAAACAT GTGAAGAGGA AATGCTTTAC TGAAGCAGCC 
ATGTGTTTGG TTCATGCTGC TGCCCTAGTG GCAGAATATT TAGGCATGTT AGAGGACTGC 
AGCTATCTGC CCGTAGGCAG TGTTAGCTTT CAGAATATTT CCTCCAATGT AATTGAGGAA 
TCCGCAGTTT CTGATGACAT TCTCACTCCA GATGATGATG GTGTGTGTTC TGGAAGATAT 
TTTTCAGAAA ATGGGTTGAT CGGACTCTTG GAGCAGGCTG CTGAACTTTT TAATACAGCA 
GCATTCTATG AAACTGTAAA TGAAGTTTAC AAAATTGTCA TCCCAGTTCT TGAGGCGCAT 
CGTGATTTCC ACAAGCTCGC ATCAACCCAT AAAAAACTTC AGAAAGCTTT TGACAATATT 
GCTGAAAAGG GTCAGAAAAG AATGTTTGGA ACATACTTCC GTGTAGGATT CTATGGGTCA 
AAGTTTGGAG ATTTAGATGA GCAAGAGTAT ATTTATAAGG AACCTACGAT TACAAAACTT 
CCTGAGATTT CCCATAGATT AGAGAGTTTT TATGGTCAGT GTTTCGGAGA TGATGCTGTA 
GAAGTTATTA AAGACTCTGT TCCTGTGGAC AAATCGAAAT TGGATCCTAA TAAGGCTTAC 
ATACAGATTA CCTTTGTGGA GCCCTATTTT GAAGACTATG AAAAGAAGTA CAGAGCCACC 
CACTTCGAGA AGAACTACAA CCTTCGTCGC TTTATGTATA CAACTCCTTT TACTCTAGAT 
GGGCGTCCCA GAGGAGAACT CAGCGAACAG TACAAGAGAA AAACCATCTT AACCACAATA 
CACGCATTTC CTTACATTAA AACACGCATC AATGTCATCC AGAAAGAGGA GTTCGAACTC 
ACTCCTATAG AGGTTGCCAT AGAAGATATG CAGAAGAAAA CACATGAATT GGCTGATGCA 
ACCAACAAGG AGCCACCTGA TGCCAAGATG CTTCAAATGT TGCTTCAAGG CTCTGTAGGA 
GCCACTGTAA ACCAGGGACC ACTAGAGGTA GCCCAAGTGT TTCTTGCTGA AATTCCAGCT 
GACCCTAAAC TCTATCGACA TCACAACAAG CTGAGATTGT GCTTTAAAGA GTTTATTATG 
CGGTGTGGTG AGGCAGTCGA AAAGAACAAA AGGCTCATCA CTGCTGACCA GAGAGAATAT 
CAGCAGGAAC TGAAGAAGAA CTATAACAAA CTGAAAGAGA ACCTGCGACC CATGATCGAG 
CGCAAAATCC CAGAGCTATA TAAGAATGTA GTCAAGACAC GTGGTGATGT CCGAGGGCTG 
TACCATAGAA CAAGCTTCAG AAAATACGAC TCCCAGCTCT CTTCCAGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808011.1
CDS	195..6368
Translation	MASLSSAERRAFALKLNRNSSAEIRKHLSLQYSGEFIPLRGLGTPTSSTLQLPPFYDVVDPVDFESFLMTHINNQDPEFRQELGDFFEDELDTVYKAKECRTVQPSLPEQGVELDPHVRDCISTYIRESLIVTRKSQENQVIGSFSRSGSRKDFPKTLQKQVFESDEAEPRPNEQNDDSPITLLIDESGQTALTSCDFDLRGLRPETRLKNLLQFSNVEDLERQNEEARKSNRQSDLFALSPPMDEEDTVEIRPVPDCPKEHMGYRIMVKLHTLKFEIDIEPLFASFALYDLKEKKKISENFHCDLNSEVYKGYLRNHIPNIATSSQAKSAVFSITYPSQDIFLVVKIEKVLQQGEISDCAEPYMVLKETESGKTKDKIEKLKSQAENFCQRLGKYRMPFAWAPVSLLNCINVMTLERDSPESENPNGRNPATDRRASWTAGRRFSDRCNSLEENTQLPFKPTTLTVNSFFKQEGDKLSDEDLFKHLADYKRSSLQRRIKSIPGLLKLEISPVCDGLSSCLSPELLPVKPEGRTRPIKEILEFPVKEVYVPHTIYRNILYVYPQRLNFANRLSSARNIAIKIQFMNGEDAKNAMPVIFGKSSGPEFLSEVTTAVTYHNKTPDFYEEVKIKLPAKLTNRHHLLFTFYHISCQSKQGACVETLLGYSWLPMLLNDRLQIGQYCLPVAMEKLPLNYSMHTPEKVPGQSPPIKWVEGHKGVFDLEVQAVSSVHTQDNHLEKFLTLCHALEGPVTFPIRVLDQKITESSLEHELKLSVVCLSSSRLEPLVLFLHLVLDKLFHLTVQPMVIGSQTANFSQYAFESVVSIVNSLHNSKDLSKDQHGRNCLLASYVYYVFRLPEVQRDTIIAAGNLPEMRYYTVGRTSAASVGSKLMQSRSLSSSNPDITGNQNNADEEVKNIMSSKHFHEEMALQMVVSTGMVRESVFKYAWFFFELLVKSMALYVHSVDKQDVPRRSRFSDRFKDDISTIVNVVTSEIGSLLSKPQKESEQAEKINISLAFFLYDLLSLMDRGFVFNLIKHYCNQLTSKLNSFPTLISMRLEFLRILCSHEHYLNLNLFFVTSSTPASPCPSISSENSSSCSSFQDQKIASMFDLSPDYRQKHFLTGLLFTELDAALDSEMEGIGKVQRKAIGAIYNLLSSHDLDPRCQRIETKQKIADLYLPLVSTILDALPQLHDFSAVDTRSGKARTSDEESETPSTINPSVAMAVAGNQFNIKTSTISSVPVKVYSTLSPESSRILLTCFLWIMKNAEQNVIQKWITYMPILQLNRILDLLYMCVSCFEYKGRQSSDKVSTLVLQKSRDVKARLEEALLRGEGARGEMMRRWKTGNERFAGLNENLRWRKEQTQWRQANERLNMTKAEIEQEAIMCRNLATEANVTILDIVETIIQTSLSIDCKDNLLGGVLKVLVHSLSTNQSNTFLTHCFSTIRLLIIKFADFLFEEEVEQCSDLCQKVLHHCCSSLESTRSQACATLYLIMRFSFSNISNFARVKMQVTMSLASLVGKSQDFNEEFLRKSLRTILAYAEEDEEMQPTLFPAQVDDLLNNLNSILSDTVKMRAFQEDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHVKRKCFTEAAMCLVHAAALVAEYLGMLEDCSYLPVGSVSFQNISSNVIEESAVSDDILTPDDDGVCSGRYFSENGLIGLLEQAAELFNTAAFYETVNEVYKIVIPVLEAHRDFHKLASTHKKLQKAFDNIAEKGQKRMFGTYFRVGFYGSKFGDLDEQEYIYKEPTITKLPEISHRLESFYGQCFGDDAVEVIKDSVPVDKSKLDPNKAYIQITFVEPYFEDYEKKYRATHFEKNYNLRRFMYTTPFTLDGRPRGELSEQYKRKTILTTIHAFPYIKTRINVIQKEEFELTPIEVAIEDMQKKTHELADATNKEPPDAKMLQMLLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVEKNKRLITADQREYQQELKKNYNKLKENLRPMIERKIPELYKNVVKTRGDVRGLYHRTSFRKYDSQLSSSG

Target ORF information:

RefSeq Version	XM_012952557.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952557.2

ORF Insert Sequence:

ATGGCTTCTC TCTCCAGTGC CGAAAGAAGA GCCTTTGCGC TGAAGCTCAA CAGGAATTCT 
TCAGCAGAAA TAAGGAAACA TCTCTCCCTT CAGTACAGTG GGGAATTTAT ACCATTGCGT 
GGCCTTGGTA CACCAACCTC TTCAACTCTA CAACTACCTC CATTTTATGA CGTGGTAGAC 
CCTGTTGATT TTGAAAGCTT CCTCATGACA CACATCAACA ACCAAGATCC TGAATTTCGG 
CAAGAATTGG GTGACTTTTT TGAAGATGAA TTGGATACTG TATACAAAGC CAAGGAGTGC 
AGGACTGTGC AGCCTTCTTT ACCAGAACAA GGAGTTGAGC TGGATCCACA CGTCCGAGAC 
TGTATCAGTA CGTATATACG TGAATCGCTG ATTGTCACCA GAAAAAGTCA AGAAAACCAA 
GTAATAGGAA GCTTTAGCAG AAGCGGATCT AGGAAAGATT TTCCCAAGAC TCTCCAAAAA 
CAAGTGTTTG AGTCAGATGA AGCAGAACCT AGACCAAACG AACAGAATGA TGATAGTCCA 
ATCACTTTGC TTATTGATGA ATCTGGACAA ACTGCACTTA CATCCTGTGA CTTTGACCTG 
CGTGGCTTAC GACCAGAAAC TCGGCTGAAG AACTTGCTTC AATTTAGTAA TGTTGAAGAT 
CTGGAGAGAC AAAATGAAGA AGCTCGCAAA TCCAATAGGC AGTCAGATCT TTTTGCTCTC 
AGTCCACCAA TGGATGAGGA GGACACCGTA GAAATACGCC CTGTGCCGGA CTGTCCAAAA 
GAACACATGG GATACAGAAT AATGGTCAAA TTGCATACTT TGAAATTTGA GATTGACATT 
GAGCCACTGT TTGCCAGCTT TGCACTCTAT GACTTAAAAG AAAAAAAAAA GATCTCTGAA 
AATTTTCACT GTGACCTGAA TTCCGAAGTG TATAAGGGTT ATCTGCGCAA TCACATTCCT 
AACATAGCCA CATCAAGCCA AGCAAAGTCT GCTGTGTTTT CAATTACCTA TCCCTCCCAA 
GATATATTCC TAGTTGTCAA GATCGAAAAA GTTCTTCAGC AGGGAGAAAT CTCAGACTGC 
GCAGAGCCAT ATATGGTTTT AAAAGAAACT GAAAGTGGAA AGACCAAAGA TAAAATTGAA 
AAACTAAAAT CTCAAGCTGA AAACTTTTGT CAGCGTCTAG GAAAGTACAG AATGCCATTT 
GCATGGGCAC CTGTGAGCCT CTTAAACTGC ATTAATGTTA TGACACTTGA AAGAGATTCC 
CCTGAGAGTG AAAACCCCAA TGGAAGAAAT CCGGCTACTG ACAGAAGAGC CTCATGGACT 
GCAGGAAGGC GTTTTTCTGA TCGATGCAAC TCATTGGAAG AGAACACCCA ATTGCCCTTC 
AAGCCTACAA CACTTACTGT CAACAGTTTC TTTAAACAGG AAGGAGACAA GCTAAGTGAC 
GAAGATTTAT TTAAACATCT AGCAGACTAC AAAAGGTCAT CTTTGCAAAG GAGAATTAAA 
TCTATACCAG GTCTACTCAA ACTGGAAATT TCTCCTGTCT GTGATGGGCT CAGCTCTTGT 
CTTTCACCTG AGTTGCTCCC AGTTAAACCT GAAGGCCGAA CACGCCCAAT TAAAGAAATA 
CTAGAGTTTC CAGTTAAAGA AGTTTATGTT CCACATACCA TTTACAGGAA TATTCTGTAT 
GTCTACCCAC AAAGGCTAAA TTTTGCAAAC CGGCTTTCAT CTGCAAGAAA CATTGCTATC 
AAAATCCAGT TCATGAATGG AGAAGATGCT AAAAACGCTA TGCCTGTCAT ATTTGGGAAG 
TCCAGTGGCC CAGAGTTTTT GTCAGAAGTC ACCACAGCTG TTACATACCA CAACAAAACT 
CCGGATTTTT ATGAAGAAGT GAAAATTAAA CTGCCTGCTA AACTCACAAA TAGACATCAC 
TTACTATTTA CATTTTACCA TATCAGCTGT CAGTCTAAAC AAGGAGCATG TGTGGAAACT 
CTGCTGGGAT ATTCATGGCT GCCAATGTTG TTAAACGATC GCCTTCAAAT AGGACAGTAT 
TGCCTTCCTG TGGCAATGGA AAAGCTGCCT CTGAATTATT CTATGCATAC TCCAGAGAAA 
GTGCCAGGGC AGTCACCCCC CATAAAATGG GTAGAAGGGC ACAAAGGCGT TTTTGACTTA 
GAGGTCCAGG CAGTCTCATC TGTGCATACT CAGGACAACC ACCTAGAGAA GTTCCTCACT 
CTTTGCCATG CTCTTGAAGG TCCAGTGACA TTTCCCATAA GAGTGCTAGA CCAGAAAATT 
ACAGAATCAT CTCTGGAACA TGAACTAAAG CTCAGCGTTG TCTGTTTAAG CTCTTCCAGA 
CTAGAGCCTC TGGTGCTTTT TCTTCATCTT GTACTGGATA AACTTTTTCA TCTTACTGTG 
CAGCCAATGG TTATTGGAAG TCAGACAGCC AACTTTTCTC AGTATGCTTT TGAATCAGTG 
GTGTCTATAG TGAACAGCCT TCACAATAGT AAAGACTTAA GCAAAGATCA GCATGGCAGA 
AACTGCTTAT TGGCCTCTTA TGTATATTAT GTATTCCGTC TTCCCGAGGT TCAGAGAGAC 
ACGATCATAG CAGCTGGAAA TCTGCCAGAG ATGCGCTACT ATACAGTAGG ACGTACATCT 
GCTGCATCCG TTGGGTCAAA ACTAATGCAG TCACGTTCAC TGAGCTCCAG TAATCCTGAT 
ATCACTGGCA ATCAGAACAA TGCAGATGAA GAAGTCAAAA ACATTATGTC CTCTAAGCAC 
TTTCATGAAG AAATGGCCCT TCAAATGGTT GTCAGCACTG GAATGGTTAG AGAAAGTGTC 
TTCAAGTATG CTTGGTTCTT CTTTGAACTG CTGGTAAAGA GCATGGCCCT ATATGTTCAC 
AGTGTTGACA AACAGGATGT CCCCAGGAGA AGTCGCTTCT CAGACCGGTT TAAAGATGAC 
ATAAGCACCA TTGTAAATGT TGTCACTTCT GAGATTGGAT CTCTCTTAAG TAAACCTCAA 
AAAGAGTCGG AACAAGCTGA GAAAATCAAC ATAAGTCTAG CATTTTTCTT ATATGATTTA 
CTATCTCTGA TGGACAGAGG TTTTGTTTTT AACCTAATCA AGCACTACTG TAATCAGCTG 
ACCTCTAAGC TGAATTCTTT CCCAACCTTG ATTTCTATGA GACTGGAGTT CCTGAGGATC 
CTTTGTAGCC ATGAGCATTA TCTCAATCTA AACCTCTTCT TTGTAACTTC CTCCACGCCT 
GCTTCTCCAT GCCCTTCTAT CTCTTCAGAG AACTCCAGCT CCTGCTCCAG TTTTCAGGAT 
CAGAAAATTG CAAGCATGTT TGACCTCTCA CCAGATTACC GGCAAAAGCA TTTTCTAACT 
GGGCTTCTGT TTACAGAGTT GGATGCTGCC CTGGACTCTG AGATGGAAGG AATTGGCAAG 
GTTCAAAGGA AAGCTATAGG TGCAATTTAC AACCTGCTGA GTTCACATGA CCTGGACCCA 
CGCTGCCAAA GAATAGAGAC AAAACAAAAA ATTGCTGATC TTTACCTGCC TTTGGTCAGT 
ACCATTTTAG ATGCACTTCC TCAACTACAT GATTTTTCAG CTGTAGACAC ACGAAGTGGA 
AAAGCCAGAA CCTCAGATGA AGAGTCAGAA ACTCCAAGCA CAATTAACCC AAGTGTAGCA 
ATGGCTGTTG CTGGAAACCA ATTCAACATA AAAACATCTA CCATATCTTC TGTGCCAGTT 
AAAGTGTACT CAACCCTAAG CCCTGAATCC AGCAGAATCC TGCTAACATG CTTCCTTTGG 
ATCATGAAGA ATGCTGAGCA GAATGTGATT CAGAAGTGGA TCACCTATAT GCCTATTCTG 
CAGCTAAACA GAATTCTGGA TCTGCTCTAC ATGTGCGTTT CCTGCTTTGA ATACAAAGGA 
AGGCAATCTT CAGACAAAGT CAGCACCTTG GTGCTTCAGA AATCAAGAGA TGTTAAGGCA 
CGCCTGGAAG AAGCATTGCT TAGGGGTGAA GGCGCCAGGG GTGAAATGAT GAGACGCTGG 
AAGACAGGGA ATGAAAGATT TGCTGGACTG AATGAAAATT TACGGTGGAG AAAAGAACAG 
ACACAGTGGC GTCAAGCTAA TGAGAGGTTA AATATGACAA AAGCTGAAAT TGAACAAGAA 
GCCATAATGT GTAGGAACCT GGCAACAGAA GCAAATGTAA CTATTTTGGA TATAGTGGAA 
ACTATCATAC AGACCAGTTT GTCCATTGAT TGCAAAGATA ATCTTCTGGG AGGAGTCCTT 
AAAGTTCTTG TACATTCCTT GAGTACGAAT CAGAGCAACA CCTTCCTTAC TCACTGTTTT 
TCAACCATCC GTCTGCTAAT TATTAAGTTT GCAGATTTTC TGTTTGAAGA AGAAGTGGAG 
CAATGCTCAG ATCTGTGTCA AAAGGTCCTA CACCACTGTT GCAGTTCCTT GGAAAGCACT 
CGGAGCCAGG CCTGTGCCAC TCTGTACCTC ATCATGAGAT TCAGCTTTAG CAATATCAGT 
AACTTTGCCA GAGTGAAGAT GCAGGTAACT ATGTCACTGG CATCCTTGGT TGGAAAATCA 
CAAGACTTCA ATGAAGAGTT CCTGCGCAAA TCATTAAGGA CGATTCTGGC CTATGCAGAA 
GAGGATGAGG AGATGCAGCC TACACTTTTC CCAGCTCAGG TGGATGACCT TTTAAATAAC 
CTGAACAGCA TTCTGTCAGA CACAGTTAAG ATGAGGGCTT TCCAGGAGGA CCCAGAAATG 
CTTATGGATT TGATGTATAG AATTGCCAAG GGCTATCAGA CCTCCCCAGA CCTTAGGCTG 
ACCTGGCTGC AGAATATGGC AGAGAAACAT GTGAAGAGGA AATGCTTTAC TGAAGCAGCC 
ATGTGTTTGG TTCATGCTGC TGCCCTAGTG GCAGAATATT TAGGCATGTT AGAGGACTGC 
AGCTATCTGC CCGTAGGCAG TGTTAGCTTT CAGAATATTT CCTCCAATGT AATTGAGGAA 
TCCGCAGTTT CTGATGACAT TCTCACTCCA GATGATGATG GTGTGTGTTC TGGAAGATAT 
TTTTCAGAAA ATGGGTTGAT CGGACTCTTG GAGCAGGCTG CTGAACTTTT TAATACAGCA 
GCATTCTATG AAACTGTAAA TGAAGTTTAC AAAATTGTCA TCCCAGTTCT TGAGGCGCAT 
CGTGATTTCC ACAAGCTCGC ATCAACCCAT AAAAAACTTC AGAAAGCTTT TGACAATATT 
GCTGAAAAGG GTCAGAAAAG AATGTTTGGA ACATACTTCC GTGTAGGATT CTATGGGTCA 
AAGTTTGGAG ATTTAGATGA GCAAGAGTAT ATTTATAAGG AACCTACGAT TACAAAACTT 
CCTGAGATTT CCCATAGATT AGAGAGTTTT TATGGTCAGT GTTTCGGAGA TGATGCTGTA 
GAAGTTATTA AAGACTCTGT TCCTGTGGAC AAATCGAAAT TGGATCCTAA TAAGGCTTAC 
ATACAGATTA CCTTTGTGGA GCCCTATTTT GAAGACTATG AAAAGAAGTA CAGAGCCACC 
CACTTCGAGA AGAACTACAA CCTTCGTCGC TTTATGTATA CAACTCCTTT TACTCTAGAT 
GGGCGTCCCA GAGGAGAACT CAGCGAACAG TACAAGAGAA AAACCATCTT AACCACAATA 
CACGCATTTC CTTACATTAA AACACGCATC AATGTCATCC AGAAAGAGGA GTTCGAACTC 
ACTCCTATAG AGGTTGCCAT AGAAGATATG CAGAAGAAAA CACATGAATT GGCTGATGCA 
ACCAACAAGG AGCCACCTGA TGCCAAGATG CTTCAAATGT TGCTTCAAGG CTCTGTAGGA 
GCCACTGTAA ACCAGGGACC ACTAGAGGTA GCCCAAGTGT TTCTTGCTGA AATTCCAGCT 
GACCCTAAAC TCTATCGACA TCACAACAAG CTGAGATTGT GCTTTAAAGA GTTTATTATG 
CGGTGTGGTG AGGCAGTCGA AAAGAACAAA AGGCTCATCA CTGCTGACCA GAGAGAATAT 
CAGCAGGAAC TGAAGAAGAA CTATAACAAA CTGAAAGAGA ACCTGCGACC CATGATCGAG 
CGCAAAATCC CAGAGCTATA TAAGAATGTA GTCAAGACAC GTGGTGATGT CCGAGGGCTG 
TACCATAGAA CAAGCTTCAG AAAATACGAC TCCCAGCTCT CTTCCAGTGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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dock8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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