prrc2b gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012968983.2	XP_012824437.1	protein PRRC2B isoform X5
XM_018096599.1	XP_017952088.1	protein PRRC2B isoform X7
XM_012968985.2	XP_012824439.1	protein PRRC2B isoform X8
XM_012968980.3	XP_012824434.2	protein PRRC2B isoform X2
XM_012968981.2	XP_012824435.1	protein PRRC2B isoform X3
XM_012968983.3	XP_012824437.2	protein PRRC2B isoform X4
XM_012968985.3	XP_012824439.2	protein PRRC2B isoform X7
XM_012968980.2	XP_012824434.1	protein PRRC2B isoform X2
XM_012968984.2	XP_012824438.1	protein PRRC2B isoform X6
XM_012968982.2	XP_012824436.1	protein PRRC2B isoform X4
XM_012968981.3	XP_012824435.2	protein PRRC2B isoform X3
XM_012968984.3	XP_012824438.1	protein PRRC2B isoform X6
XM_012968979.2	XP_012824433.1	protein PRRC2B isoform X1
XM_031890906.1	XP_031746766.1	protein PRRC2B isoform X1
XM_012968982.3	XP_012824436.1	protein PRRC2B isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa64142	XM_012968985.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa35277	XM_012968985.2 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa35274	XM_012968982.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa35276	XM_012968984.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64138	XM_031890906.1 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64139	XM_012968980.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64140	XM_012968981.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa64141	XM_012968983.3 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa35271	XM_012968979.2 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa35272	XM_012968980.2 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa35273	XM_012968981.2 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa35275	XM_012968983.2 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa35276	XM_018096599.1 Latest version!	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OXa35276	XM_012968984.2	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa35274	XM_012968982.2	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

prrc2b ( XM_012968985.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824439.2 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa64142
Clone ID Related Accession (Same CDS sequence)	XM_012968985.3
Accession Version	XM_012968985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968985.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGCCGCTGCT CTGACGATTC CTATGGTGCA 
GAGAGCAGCC ACCACCGTTA CGCCATGGAC AGGTCTATGC ACTGCGACAG CGGAGAGGCG 
GGGAAGAAAC AGGAAAGGGA TTCAAGGACG CCTGGTCTCA AATCCAGTGA TAAAAATGAG 
GTGATCGGAC AGTTTGACCT CAAATATGGA GATTCCATGC TTGAGGAAGA GTCCGAGGTC 
GGAGACATTG GTAACAAGCC TCGTCGTGTG TTGGAAAAGG ATCGCCGGAA AAAGGACCAG 
GTGGTTCAGG TGCCGGCAAA GGGCAGCAGT ATCCAGTCTA GGATTCCCCC TCGCTTTGCC 
AAAAAACAGA ACGGCATGTG CCTGGATCAA GCCGACGTTA CTGCCAAAGA AATCTGGGAG 
AGCAGCGGCC AAGGAATCTC TGTCCAGCCT GGCACGGATA CCTGGAGTAA ACCTGTGAGC 
TCATTTAGCA CGGAGTCCAG CTCCACAGAG GGCTTTAAAG GCAGTCAAGG AGACAGTGGC 
ATTGACCTGA GTGCCGAGTC TCGGGAGTCG TCTGCTACAT CTTCACAACG CAGCTCTCCG 
TATGGAACCA TGAAACCGGA GGAAATGAAC GGAACAGGTG TGGCAGAGCC AAAACCAGAT 
TGCCCTAAAG AACAAGGGCA AAAACAGTCT GAGAAAAAGG ATTCCGACCC TGGCTCGGGC 
CTAAATAAAG AACATAAACC TGGACCCATT GGCAACGAAC GCTCGCTGAA AAACCGAAAA 
GGATCCGAGG GCACCGAACG GTTGGAAGGC GGCGTCCCCC CAGTCAATGG AGTAGAGATC 
CACGTGGATT CGGTTCTCCC TGTTCCGCCT ATTGAATTTG GAGTAAGCGC AAAAGATGCA 
GACTATGCCC TCCCTCCTGG TACTGCCACC GTTCAAGCAG CCAATACTGT TACAAAGTTG 
CAGGATGCTT TGGCCAGTAA GGCAGGGCTA ACGCAGTCCA TCCCCATCCT AAGGAGAGAC 
CACCACATGC AGCAGGGTAT GAGCCTAAAC ATGTCCTACC CCACTGCGGA TCTCACCCTT 
AAGATGGAGT CTGCCCGCAA AGCCTGGGAA AACTCGCCCA GTTTGCCAGA GCAGAATTCG 
CCAGCAGGCC CTGGCTCAGG AATTCAGCCG CCATCTAGTG TGGGAGCCTC CACCGGGGTC 
AACTACAGCT CCTTTGGCGG CGTGTCAATG CCACCCATGC CTGTTGCCTC TGTCGCCCCG 
TCTGCCTCCA TGCCAGGAAA CCACATCCCA CCCCTGTACC TTGATGGGCA CGTGTTTACC 
AACCAGCCGC GCCTGGTGCA GCAAACCATA CCTCAACAGC AAGGGTATCA GCAGGCAGCA 
GCTGCCCAGC AGATCCCCAT TTCCCTGCAC ACCTCCCTGC AGGCTCAAGC CCAGCTGGGT 
TTGCGAGGAG GCCTCCCCGT GTCCCAGTCG CAAGAAATTT ACAGCTCCAT GCAGCCGTTC 
AGGTCCCAGG TGTACATGCA TCCAAGTCTT TCTCAGCCCA GTGCTATGGT ACTGACCAGT 
GGCACAGGCC TCAAGCCCCC ATACAACCCT TTCCCTGGCA TGCAGACACT GGAAATGGTT 
AAAACCCAGC CCACGTCCCC TTATCAGCCC CTGAATGGGA GCCAGCAGCT GGTGTATGAA 
AGCCAACTGA ACCAGGCCTC CCAGATGATG GACTCCCAGC TCACACAGTT GACCATGCCC 
ATGCCCGGCT CCCAGCTCCA GATGCCCCGA TACAGTTCAG GACAGCAAAC AATGATCTTA 
CCACAGTCCA TACAGCTTCC CCAGGGGCAG AACCTGCCTG TAGGGGCTCC TCGGAGAATG 
CAGCCTTCTA TGCTGACAAG CAGAGAGTCC TCCCAGATGG AGATGAAAAG CTTCCACTTT 
ACAGACGGCA AACAGAACAT GCCGACTGCC ATGCAAGCAC AGCACTCCTA TCGGCCGAGT 
TCAGCTAGCC CCAGTGGAAA GTCGCCCGGA CCGGGGCCAT CTGCCAACTT GGGCTCTGTG 
CAGGGACACT ACCCTCAACA GGTAAAACAC AGATCCGACG ACAGCAAAGG CGGCCTGAGG 
TCCCCCAAAG TTCCCGAGCA GCCCTCAGCG AGTCCCATGA AGCCGGCCAG GACGGGGGCC 
ATCAAGCCTC AAGCGGTGAA AGTGGAAGAG AGCAAGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824439.2
CDS	319..4860
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKHRSDDSKGGLRSPKVPEQPSASPMKPARTGAIKPQAVKVEESKA

Target ORF information:

RefSeq Version	XM_012968985.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968985.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGCCGCTGCT CTGACGATTC CTATGGTGCA 
GAGAGCAGCC ACCACCGTTA CGCCATGGAC AGGTCTATGC ACTGCGACAG CGGAGAGGCG 
GGGAAGAAAC AGGAAAGGGA TTCAAGGACG CCTGGTCTCA AATCCAGTGA TAAAAATGAG 
GTGATCGGAC AGTTTGACCT CAAATATGGA GATTCCATGC TTGAGGAAGA GTCCGAGGTC 
GGAGACATTG GTAACAAGCC TCGTCGTGTG TTGGAAAAGG ATCGCCGGAA AAAGGACCAG 
GTGGTTCAGG TGCCGGCAAA GGGCAGCAGT ATCCAGTCTA GGATTCCCCC TCGCTTTGCC 
AAAAAACAGA ACGGCATGTG CCTGGATCAA GCCGACGTTA CTGCCAAAGA AATCTGGGAG 
AGCAGCGGCC AAGGAATCTC TGTCCAGCCT GGCACGGATA CCTGGAGTAA ACCTGTGAGC 
TCATTTAGCA CGGAGTCCAG CTCCACAGAG GGCTTTAAAG GCAGTCAAGG AGACAGTGGC 
ATTGACCTGA GTGCCGAGTC TCGGGAGTCG TCTGCTACAT CTTCACAACG CAGCTCTCCG 
TATGGAACCA TGAAACCGGA GGAAATGAAC GGAACAGGTG TGGCAGAGCC AAAACCAGAT 
TGCCCTAAAG AACAAGGGCA AAAACAGTCT GAGAAAAAGG ATTCCGACCC TGGCTCGGGC 
CTAAATAAAG AACATAAACC TGGACCCATT GGCAACGAAC GCTCGCTGAA AAACCGAAAA 
GGATCCGAGG GCACCGAACG GTTGGAAGGC GGCGTCCCCC CAGTCAATGG AGTAGAGATC 
CACGTGGATT CGGTTCTCCC TGTTCCGCCT ATTGAATTTG GAGTAAGCGC AAAAGATGCA 
GACTATGCCC TCCCTCCTGG TACTGCCACC GTTCAAGCAG CCAATACTGT TACAAAGTTG 
CAGGATGCTT TGGCCAGTAA GGCAGGGCTA ACGCAGTCCA TCCCCATCCT AAGGAGAGAC 
CACCACATGC AGCAGGGTAT GAGCCTAAAC ATGTCCTACC CCACTGCGGA TCTCACCCTT 
AAGATGGAGT CTGCCCGCAA AGCCTGGGAA AACTCGCCCA GTTTGCCAGA GCAGAATTCG 
CCAGCAGGCC CTGGCTCAGG AATTCAGCCG CCATCTAGTG TGGGAGCCTC CACCGGGGTC 
AACTACAGCT CCTTTGGCGG CGTGTCAATG CCACCCATGC CTGTTGCCTC TGTCGCCCCG 
TCTGCCTCCA TGCCAGGAAA CCACATCCCA CCCCTGTACC TTGATGGGCA CGTGTTTACC 
AACCAGCCGC GCCTGGTGCA GCAAACCATA CCTCAACAGC AAGGGTATCA GCAGGCAGCA 
GCTGCCCAGC AGATCCCCAT TTCCCTGCAC ACCTCCCTGC AGGCTCAAGC CCAGCTGGGT 
TTGCGAGGAG GCCTCCCCGT GTCCCAGTCG CAAGAAATTT ACAGCTCCAT GCAGCCGTTC 
AGGTCCCAGG TGTACATGCA TCCAAGTCTT TCTCAGCCCA GTGCTATGGT ACTGACCAGT 
GGCACAGGCC TCAAGCCCCC ATACAACCCT TTCCCTGGCA TGCAGACACT GGAAATGGTT 
AAAACCCAGC CCACGTCCCC TTATCAGCCC CTGAATGGGA GCCAGCAGCT GGTGTATGAA 
AGCCAACTGA ACCAGGCCTC CCAGATGATG GACTCCCAGC TCACACAGTT GACCATGCCC 
ATGCCCGGCT CCCAGCTCCA GATGCCCCGA TACAGTTCAG GACAGCAAAC AATGATCTTA 
CCACAGTCCA TACAGCTTCC CCAGGGGCAG AACCTGCCTG TAGGGGCTCC TCGGAGAATG 
CAGCCTTCTA TGCTGACAAG CAGAGAGTCC TCCCAGATGG AGATGAAAAG CTTCCACTTT 
ACAGACGGCA AACAGAACAT GCCGACTGCC ATGCAAGCAC AGCACTCCTA TCGGCCGAGT 
TCAGCTAGCC CCAGTGGAAA GTCGCCCGGA CCGGGGCCAT CTGCCAACTT GGGCTCTGTG 
CAGGGACACT ACCCTCAACA GGTAAAACAC AGATCCGACG ACAGCAAAGG CGGCCTGAGG 
TCCCCCAAAG TTCCCGAGCA GCCCTCAGCG AGTCCCATGA AGCCGGCCAG GACGGGGGCC 
ATCAAGCCTC AAGCGGTGAA AGTGGAAGAG AGCAAGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968985.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824439.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa35277
Clone ID Related Accession (Same CDS sequence)	XM_012968985.2
Accession Version	XM_012968985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGCCGCTGCT CTGACGATTC CTATGGTGCA 
GAGAGCAGCC ACCACCGTTA CGCCATGGAC AGGTCTATGC ACTGCGACAG CGGAGAGGCG 
GGGAAGAAAC AGGAAAGGGA TTCAAGGACG CCTGGTCTCA AATCCAGTGA TAAAAATGAG 
GTGATCGGAC AGTTTGACCT CAAATATGGA GATTCCATGC TTGAGGAAGA GTCCGAGGTC 
GGAGACATTG GTAACAAGCC TCGTCGTGTG TTGGAAAAGG ATCGCCGGAA AAAGGACCAG 
GTGGTTCAGG TGCCGGCAAA GGGCAGCAGT ATCCAGTCTA GGATTCCCCC TCGCTTTGCC 
AAAAAACAGA ACGGCATGTG CCTGGATCAA GCCGACGTTA CTGCCAAAGA AATCTGGGAG 
AGCAGCGGCC AAGGAATCTC TGTCCAGCCT GGCACGGATA CCTGGAGTAA ACCTGTGAGC 
TCATTTAGCA CGGAGTCCAG CTCCACAGAG GGCTTTAAAG GCAGTCAAGG AGACAGTGGC 
ATTGACCTGA GTGCCGAGTC TCGGGAGTCG TCTGCTACAT CTTCACAACG CAGCTCTCCG 
TATGGAACCA TGAAACCGGA GGAAATGAAC GGAACAGGTG TGGCAGAGCC AAAACCAGAT 
TGCCCTAAAG AACAAGGGCA AAAACAGTCT GAGAAAAAGG ATTCCGACCC TGGCTCGGGC 
CTAAATAAAG AACATAAACC TGGACCCATT GGCAACGAAC GCTCGCTGAA AAACCGAAAA 
GGATCCGAGG GCACCGAACG GTTGGAAGGC GGCGTCCCCC CAGTCAATGG AGTAGAGATC 
CACGTGGATT CGGTTCTCCC TGTTCCGCCT ATTGAATTTG GAGTAAGCGC AAAAGATGCA 
GACTATGCCC TCCCTCCTGG TACTGCCACC GTTCAAGCAG CCAATACTGT TACAAAGTTG 
CAGGATGCTT TGGCCAGTAA GGCAGGGCTA ACGCAGTCCA TCCCCATCCT AAGGAGAGAC 
CACCACATGC AGCAGGGTAT GAGCCTAAAC ATGTCCTACC CCACTGCGGA TCTCACCCTT 
AAGATGGAGT CTGCCCGCAA AGCCTGGGAA AACTCGCCCA GTTTGCCAGA GCAGAATTCG 
CCAGCAGGCC CTGGCTCAGG AATTCAGCCG CCATCTAGTG TGGGAGCCTC CACCGGGGTC 
AACTACAGCT CCTTTGGCGG CGTGTCAATG CCACCCATGC CTGTTGCCTC TGTCGCCCCG 
TCTGCCTCCA TGCCAGGAAA CCACATCCCA CCCCTGTACC TTGATGGGCA CGTGTTTACC 
AACCAGCCGC GCCTGGTGCA GCAAACCATA CCTCAACAGC AAGGGTATCA GCAGGCAGCA 
GCTGCCCAGC AGATCCCCAT TTCCCTGCAC ACCTCCCTGC AGGCTCAAGC CCAGCTGGGT 
TTGCGAGGAG GCCTCCCCGT GTCCCAGTCG CAAGAAATTT ACAGCTCCAT GCAGCCGTTC 
AGGTCCCAGG TGTACATGCA TCCAAGTCTT TCTCAGCCCA GTGCTATGGT ACTGACCAGT 
GGCACAGGCC TCAAGCCCCC ATACAACCCT TTCCCTGGCA TGCAGACACT GGAAATGGTT 
AAAACCCAGC CCACGTCCCC TTATCAGCCC CTGAATGGGA GCCAGCAGCT GGTGTATGAA 
AGCCAACTGA ACCAGGCCTC CCAGATGATG GACTCCCAGC TCACACAGTT GACCATGCCC 
ATGCCCGGCT CCCAGCTCCA GATGCCCCGA TACAGTTCAG GACAGCAAAC AATGATCTTA 
CCACAGTCCA TACAGCTTCC CCAGGGGCAG AACCTGCCTG TAGGGGCTCC TCGGAGAATG 
CAGCCTTCTA TGCTGACAAG CAGAGAGTCC TCCCAGATGG AGATGAAAAG CTTCCACTTT 
ACAGACGGCA AACAGAACAT GCCGACTGCC ATGCAAGCAC AGCACTCCTA TCGGCCGAGT 
TCAGCTAGCC CCAGTGGAAA GTCGCCCGGA CCGGGGCCAT CTGCCAACTT GGGCTCTGTG 
CAGGGACACT ACCCTCAACA GGTAAAGACC AGTCCTGTCA CAAAGAGCGA ACCCATATTG 
TACCATCTCG TTCATGTTCA TGGCATCCTA GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824439.1
CDS	319..4794
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKTSPVTKSEPILYHLVHVHGILV

Target ORF information:

RefSeq Version	XM_012968985.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968985.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGCCGCTGCT CTGACGATTC CTATGGTGCA 
GAGAGCAGCC ACCACCGTTA CGCCATGGAC AGGTCTATGC ACTGCGACAG CGGAGAGGCG 
GGGAAGAAAC AGGAAAGGGA TTCAAGGACG CCTGGTCTCA AATCCAGTGA TAAAAATGAG 
GTGATCGGAC AGTTTGACCT CAAATATGGA GATTCCATGC TTGAGGAAGA GTCCGAGGTC 
GGAGACATTG GTAACAAGCC TCGTCGTGTG TTGGAAAAGG ATCGCCGGAA AAAGGACCAG 
GTGGTTCAGG TGCCGGCAAA GGGCAGCAGT ATCCAGTCTA GGATTCCCCC TCGCTTTGCC 
AAAAAACAGA ACGGCATGTG CCTGGATCAA GCCGACGTTA CTGCCAAAGA AATCTGGGAG 
AGCAGCGGCC AAGGAATCTC TGTCCAGCCT GGCACGGATA CCTGGAGTAA ACCTGTGAGC 
TCATTTAGCA CGGAGTCCAG CTCCACAGAG GGCTTTAAAG GCAGTCAAGG AGACAGTGGC 
ATTGACCTGA GTGCCGAGTC TCGGGAGTCG TCTGCTACAT CTTCACAACG CAGCTCTCCG 
TATGGAACCA TGAAACCGGA GGAAATGAAC GGAACAGGTG TGGCAGAGCC AAAACCAGAT 
TGCCCTAAAG AACAAGGGCA AAAACAGTCT GAGAAAAAGG ATTCCGACCC TGGCTCGGGC 
CTAAATAAAG AACATAAACC TGGACCCATT GGCAACGAAC GCTCGCTGAA AAACCGAAAA 
GGATCCGAGG GCACCGAACG GTTGGAAGGC GGCGTCCCCC CAGTCAATGG AGTAGAGATC 
CACGTGGATT CGGTTCTCCC TGTTCCGCCT ATTGAATTTG GAGTAAGCGC AAAAGATGCA 
GACTATGCCC TCCCTCCTGG TACTGCCACC GTTCAAGCAG CCAATACTGT TACAAAGTTG 
CAGGATGCTT TGGCCAGTAA GGCAGGGCTA ACGCAGTCCA TCCCCATCCT AAGGAGAGAC 
CACCACATGC AGCAGGGTAT GAGCCTAAAC ATGTCCTACC CCACTGCGGA TCTCACCCTT 
AAGATGGAGT CTGCCCGCAA AGCCTGGGAA AACTCGCCCA GTTTGCCAGA GCAGAATTCG 
CCAGCAGGCC CTGGCTCAGG AATTCAGCCG CCATCTAGTG TGGGAGCCTC CACCGGGGTC 
AACTACAGCT CCTTTGGCGG CGTGTCAATG CCACCCATGC CTGTTGCCTC TGTCGCCCCG 
TCTGCCTCCA TGCCAGGAAA CCACATCCCA CCCCTGTACC TTGATGGGCA CGTGTTTACC 
AACCAGCCGC GCCTGGTGCA GCAAACCATA CCTCAACAGC AAGGGTATCA GCAGGCAGCA 
GCTGCCCAGC AGATCCCCAT TTCCCTGCAC ACCTCCCTGC AGGCTCAAGC CCAGCTGGGT 
TTGCGAGGAG GCCTCCCCGT GTCCCAGTCG CAAGAAATTT ACAGCTCCAT GCAGCCGTTC 
AGGTCCCAGG TGTACATGCA TCCAAGTCTT TCTCAGCCCA GTGCTATGGT ACTGACCAGT 
GGCACAGGCC TCAAGCCCCC ATACAACCCT TTCCCTGGCA TGCAGACACT GGAAATGGTT 
AAAACCCAGC CCACGTCCCC TTATCAGCCC CTGAATGGGA GCCAGCAGCT GGTGTATGAA 
AGCCAACTGA ACCAGGCCTC CCAGATGATG GACTCCCAGC TCACACAGTT GACCATGCCC 
ATGCCCGGCT CCCAGCTCCA GATGCCCCGA TACAGTTCAG GACAGCAAAC AATGATCTTA 
CCACAGTCCA TACAGCTTCC CCAGGGGCAG AACCTGCCTG TAGGGGCTCC TCGGAGAATG 
CAGCCTTCTA TGCTGACAAG CAGAGAGTCC TCCCAGATGG AGATGAAAAG CTTCCACTTT 
ACAGACGGCA AACAGAACAT GCCGACTGCC ATGCAAGCAC AGCACTCCTA TCGGCCGAGT 
TCAGCTAGCC CCAGTGGAAA GTCGCCCGGA CCGGGGCCAT CTGCCAACTT GGGCTCTGTG 
CAGGGACACT ACCCTCAACA GGTAAAGACC AGTCCTGTCA CAAAGAGCGA ACCCATATTG 
TACCATCTCG TTCATGTTCA TGGCATCCTA GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968982.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824436.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

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CloneID	OXa35274
Clone ID Related Accession (Same CDS sequence)	XM_012968982.2 , XM_012968982.3
Accession Version	XM_012968982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TGGCAAGGTG ACCTCTTCCA GATAAAGGAG ACTCCTCCCA TCTGTTGGCG GAAGGTTCTG 
CATGCCCGGC CCGTGTCCCC TGCACAAATG GCTTTGGAAT TACCATTCTC TGCTCTCGCC 
CCCAACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824436.1
CDS	319..6750
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRWQGDLFQIKETPPICWRKVLHARPVSPAQMALELPFSALAPNR

Target ORF information:

RefSeq Version	XM_012968982.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968982.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TGGCAAGGTG ACCTCTTCCA GATAAAGGAG ACTCCTCCCA TCTGTTGGCG GAAGGTTCTG 
CATGCCCGGC CCGTGTCCCC TGCACAAATG GCTTTGGAAT TACCATTCTC TGCTCTCGCC 
CCCAACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968984.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824438.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.

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CloneID	OXa35276
Clone ID Related Accession (Same CDS sequence)	XM_018096599.1 , XM_012968984.2 , XM_012968984.3
Accession Version	XM_012968984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968984.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824438.1
CDS	319..6621
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYR

Target ORF information:

RefSeq Version	XM_012968984.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968984.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_031890906.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746766.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

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CloneID	OXa64138
Clone ID Related Accession (Same CDS sequence)	XM_031890906.1
Accession Version	XM_031890906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
CCGAGTTCAG CTAGCCCCAG TGGAAAGTCG CCCGGACCGG GGCCATCTGC CAACTTGGGC 
TCTGTGCAGG GACACTACCC TCAACAGGTA AAACACAGAT CCGACGACAG CAAAGGCGGC 
CTGAGGTCCC CCAAAGTTCC CGAGCAGCCC TCAGCGAGTC CCATGAAGCC GGCCAGGACG 
GGGGCCATCA AGCCTCAAGC GGTGAAAGTG GAAGAGAGCA AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746766.1
CDS	319..6846
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKHRSDDSKGGLRSPKVPEQPSASPMKPARTGAIKPQAVKVEESKA

Target ORF information:

RefSeq Version	XM_031890906.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890906.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
CCGAGTTCAG CTAGCCCCAG TGGAAAGTCG CCCGGACCGG GGCCATCTGC CAACTTGGGC 
TCTGTGCAGG GACACTACCC TCAACAGGTA AAACACAGAT CCGACGACAG CAAAGGCGGC 
CTGAGGTCCC CCAAAGTTCC CGAGCAGCCC TCAGCGAGTC CCATGAAGCC GGCCAGGACG 
GGGGCCATCA AGCCTCAAGC GGTGAAAGTG GAAGAGAGCA AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968980.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824434.2 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	OXa64139
Clone ID Related Accession (Same CDS sequence)	XM_012968980.3
Accession Version	XM_012968980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGA TGCAGACTAT 
GCCCTCCCTC CTGGTACTGC CACCGTTCAA GCAGCCAATA CTGTTACAAA GTTGCAGGAT 
GCTTTGGCCA GTAAGGCAGG GCTAACGCAG TCCATCCCCA TCCTAAGGAG AGACCACCAC 
ATGCAGCAGG GTATGAGCCT AAACATGTCC TACCCCACTG CGGATCTCAC CCTTAAGATG 
GAGTCTGCCC GCAAAGCCTG GGAAAACTCG CCCAGTTTGC CAGAGCAGAA TTCGCCAGCA 
GGCCCTGGCT CAGGAATTCA GCCGCCATCT AGTGTGGGAG CCTCCACCGG GGTCAACTAC 
AGCTCCTTTG GCGGCGTGTC AATGCCACCC ATGCCTGTTG CCTCTGTCGC CCCGTCTGCC 
TCCATGCCAG GAAACCACAT CCCACCCCTG TACCTTGATG GGCACGTGTT TACCAACCAG 
CCGCGCCTGG TGCAGCAAAC CATACCTCAA CAGCAAGGGT ATCAGCAGGC AGCAGCTGCC 
CAGCAGATCC CCATTTCCCT GCACACCTCC CTGCAGGCTC AAGCCCAGCT GGGTTTGCGA 
GGAGGCCTCC CCGTGTCCCA GTCGCAAGAA ATTTACAGCT CCATGCAGCC GTTCAGGTCC 
CAGGTGTACA TGCATCCAAG TCTTTCTCAG CCCAGTGCTA TGGTACTGAC CAGTGGCACA 
GGCCTCAAGC CCCCATACAA CCCTTTCCCT GGCATGCAGA CACTGGAAAT GGTTAAAACC 
CAGCCCACGT CCCCTTATCA GCCCCTGAAT GGGAGCCAGC AGCTGGTGTA TGAAAGCCAA 
CTGAACCAGG CCTCCCAGAT GATGGACTCC CAGCTCACAC AGTTGACCAT GCCCATGCCC 
GGCTCCCAGC TCCAGATGCC CCGATACAGT TCAGGACAGC AAACAATGAT CTTACCACAG 
TCCATACAGC TTCCCCAGGG GCAGAACCTG CCTGTAGGGG CTCCTCGGAG AATGCAGCCT 
TCTATGCTGA CAAGCAGAGA GTCCTCCCAG ATGGAGATGA AAAGCTTCCA CTTTACAGAC 
GGCAAACAGA ACATGCCGAC TGCCATGCAA GCACAGCACT CCTATCGGCC GAGTTCAGCT 
AGCCCCAGTG GAAAGTCGCC CGGACCGGGG CCATCTGCCA ACTTGGGCTC TGTGCAGGGA 
CACTACCCTC AACAGGTAAA ACACAGATCC GACGACAGCA AAGGCGGCCT GAGGTCCCCC 
AAAGTTCCCG AGCAGCCCTC AGCGAGTCCC ATGAAGCCGG CCAGGACGGG GGCCATCAAG 
CCTCAAGCGG TGAAAGTGGA AGAGAGCAAG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824434.2
CDS	319..6834
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKHRSDDSKGGLRSPKVPEQPSASPMKPARTGAIKPQAVKVEESKA

Target ORF information:

RefSeq Version	XM_012968980.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968980.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGA TGCAGACTAT 
GCCCTCCCTC CTGGTACTGC CACCGTTCAA GCAGCCAATA CTGTTACAAA GTTGCAGGAT 
GCTTTGGCCA GTAAGGCAGG GCTAACGCAG TCCATCCCCA TCCTAAGGAG AGACCACCAC 
ATGCAGCAGG GTATGAGCCT AAACATGTCC TACCCCACTG CGGATCTCAC CCTTAAGATG 
GAGTCTGCCC GCAAAGCCTG GGAAAACTCG CCCAGTTTGC CAGAGCAGAA TTCGCCAGCA 
GGCCCTGGCT CAGGAATTCA GCCGCCATCT AGTGTGGGAG CCTCCACCGG GGTCAACTAC 
AGCTCCTTTG GCGGCGTGTC AATGCCACCC ATGCCTGTTG CCTCTGTCGC CCCGTCTGCC 
TCCATGCCAG GAAACCACAT CCCACCCCTG TACCTTGATG GGCACGTGTT TACCAACCAG 
CCGCGCCTGG TGCAGCAAAC CATACCTCAA CAGCAAGGGT ATCAGCAGGC AGCAGCTGCC 
CAGCAGATCC CCATTTCCCT GCACACCTCC CTGCAGGCTC AAGCCCAGCT GGGTTTGCGA 
GGAGGCCTCC CCGTGTCCCA GTCGCAAGAA ATTTACAGCT CCATGCAGCC GTTCAGGTCC 
CAGGTGTACA TGCATCCAAG TCTTTCTCAG CCCAGTGCTA TGGTACTGAC CAGTGGCACA 
GGCCTCAAGC CCCCATACAA CCCTTTCCCT GGCATGCAGA CACTGGAAAT GGTTAAAACC 
CAGCCCACGT CCCCTTATCA GCCCCTGAAT GGGAGCCAGC AGCTGGTGTA TGAAAGCCAA 
CTGAACCAGG CCTCCCAGAT GATGGACTCC CAGCTCACAC AGTTGACCAT GCCCATGCCC 
GGCTCCCAGC TCCAGATGCC CCGATACAGT TCAGGACAGC AAACAATGAT CTTACCACAG 
TCCATACAGC TTCCCCAGGG GCAGAACCTG CCTGTAGGGG CTCCTCGGAG AATGCAGCCT 
TCTATGCTGA CAAGCAGAGA GTCCTCCCAG ATGGAGATGA AAAGCTTCCA CTTTACAGAC 
GGCAAACAGA ACATGCCGAC TGCCATGCAA GCACAGCACT CCTATCGGCC GAGTTCAGCT 
AGCCCCAGTG GAAAGTCGCC CGGACCGGGG CCATCTGCCA ACTTGGGCTC TGTGCAGGGA 
CACTACCCTC AACAGGTAAA ACACAGATCC GACGACAGCA AAGGCGGCCT GAGGTCCCCC 
AAAGTTCCCG AGCAGCCCTC AGCGAGTCCC ATGAAGCCGG CCAGGACGGG GGCCATCAAG 
CCTCAAGCGG TGAAAGTGGA AGAGAGCAAG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968981.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824435.2 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

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CloneID	OXa64140
Clone ID Related Accession (Same CDS sequence)	XM_012968981.3
Accession Version	XM_012968981.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968981.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAATGTCAC CAGTTGGAGA GACGGCGGTG GGAGAAGCAT CGCTTCCAGC 
TTATCTCCGA CCGCCTCTCC TACTGAGCCG GGCAGCAAAA CGGCAGTACC GGGAGAAGGG 
CCCCCCTCTA CATCGCTTGC TAATGATACT AAAGAACTCT CGCTGCGCCC GGCTCAGCCC 
ACCCGAAAAG GCCCTGCTCA GTTCATGGGA AACACCTACC ACCCACCGAC TTACCATGAC 
ATGCTACCAG CCTTTATGTG CTCTCAGCAC CCACCAGAGC CTTCTGGGAC ATTGGATCGT 
ACTTCCTTTC CCACCCTTAA TTCTCAGTCT CGCCTTGAAC CTCGAGTCCC TTTTCGCCAA 
TTTCAGATGA ATGATAACGA AGGACGGGAG AGCAGGTTTC CCATTGCACC CCCTCGTTCT 
GCACGACCCC CCCGCCCACC AGCCGAGCGC CCCCCAAGAG CCACCATCAT CAATGCAGAG 
GATCTAAAGG AGCTGGATGA CCTTGATAAT GATGCAGAGG ATGGCTGGGC TGGTCTGCAC 
GATGAGGTTG ATTATTCCGA GAAACTGAAG TTCAGTGAAG ATGAAGAAGA GGAGGACGGT 
GCTGCAAAAG ATGGCAGAAG TAAATGGAAT GGTTGGGATG GCAAAAGGCA GCGGCAGGTT 
TCCTTCAATT CTACTGATAG CGTGGAGGGA AAACATCCAG CAGATGAAGG AAAGGTGTGG 
AACGACACAT TGTCCCATTC GCGTCCTGCA AGAAGAACAC CGGAACCTGC GCCACAAGGG 
CCACGTAAAC CCAACACTTG GGCTGCTGTG GCTGATCATC AGAAACCCCC TGCCCCTGCT 
GCAGTCCTGC GACAGCCCTC CATAGAAGAG AAAGATGAAA AGCTGCCCCA GAGGAAAAAT 
TTTGTCCAGT CCGAGATCTC TGAGGCAGTA GAGCGTGCCC GCAAAAGGAG AGAGGAGGAG 
GAGAGGCGAA ACCGCGAGGA ACGGCTGGCT GCTTGTGCTG CTAAACTAAA ACAGCTTGAT 
CAGAAGAACA AGCAGGCCCT GAAACCATCG GCAGATTCTT CCCGCCAGCT GGACAATAAG 
GAGAATGAAG ACCCCCGTTC TCCTGTGTCA GACAGAAGCA ACCCCCAGGA AAACGCACAT 
ACTTTCAGAA GAGAATACTC TCAGGAGTCC CCTTCTGAAT ATCCAGAGGA GGAGCCTTCT 
GGAACCCTAA AAGCTGAAAC CAGCAGCGAA GAAGAATATG CGGAACCTAC ATCCCCAGCA 
CAAGATTTCA GCAAACATCA GAAACTGATT CCACCCCGAT TCCAGCGGCA GCAACAGCAG 
CAGGAGCAGT TGTACAAGAT GCAGCAGTGG CAGCAGCAGC AACAGCAAGC CTATGTGGCC 
TCCACTCATT CCAACCCTCC CCGGACCTTC TACTCCCCTC ACCCACAGAT GTTGGGCTTT 
GATCCACGCT GGATGATGAT GCCTTCTTAC ATGGACCCAC GGATGACGCA AGGCCGTGCG 
CCTGTGGACT TCTACCCTTC CTCCATCCAC CCTCCAGGTG TAATGAAGCA TATGATCCAG 
CAGGATTCAA TGAGCGGTTC TGGAAGTTGC CATTCAGACG AACAGAACTG CCAGTCTGAG 
AGGAGACCTC AGTCAGCAGA ACCGATGTCT GGTTGGGGCC AGGAAAGCTA CGTGCAATTA 
CACAGCAAGG CCTGCTCCCT GCCTCAGCAG AAACAGGCTG AGACTGCCGC AGTGGGGAGC 
TACAGTAGGA ATGAAAGTTC TTACTCATCC GCAAGAGAGA AATCAGATGG CTCCCCTACC 
CACCCTGAGC CCTTAGAGAG GGAAGAGGAG TATTTATCTG TTTCTTATGA AAAGAAATCC 
ACTGGGAGCT TTAGCAGTTG CATTTCCCCT CAAAGGAGAG GACAGGACGC CTTGTACCAA 
CATCTAGAGA GCGGCATAGA GTCTGGTGTG AACAGGGGGA GACAGATGGC AGCCAGGGAT 
TCGTCCTCAA GAGGCTCTGA GTTTATAAAG ACTGATAAAA AGCCACAGTT TAATGGCTGG 
GGTTATGCAC CCCATCAGAA ATCTTCCGAA ACAGCCAGCA GAGCAGAAGA GGATCTTTCA 
AAGGAGGACC TATCTGATGA ACTGGAGCAC TGGAAGAAGG AGGATGGTGG GAGCAGCCAG 
GATGGTACAG GAGAAACCTG CTGGAGAAGT GAATCTGCAA ATTCCAACAG TCAGCAGCAA 
TCTGAGCATC TAGGGAGAAG CCGTAGATCT GGACCTATCA AAAAGCCCGT CTTGAAAGCC 
CTCAAGATAG AGGACAAAGA GCTGGAGAAA AACAAGGCAG AAATTAAAGA GCCAGTAAAA 
CCTTTTAAGG AAAAAATTCT TCCAAAAACA GAGTGCGTTG TTAAGACTGA ACCTGTTGTT 
TCAAGCCCTC CTCCCAGCAT GATTGAAGTT AAAACTCCCC CTCCGCCTAG TCCTGTCCAG 
GAGACTGATA AATTCCCCCA AGAAAAAGCT GAAAGGAGTT GGGAGACCAA GCCTTTACGG 
GAATCTAGTA GCACTTCTTT GCAAAAGAGA AGTGACTGGA TATTCATTGA CGAAGAGCAA 
GCGTTTGGCG GTAGGACTCA AGGCCGTGGC AGAGGTAGAG GGTTTAAAGA CTTTGGTTTC 
CGCAACCGAG GGGTCTCTGG CACTTACAGC GGGCAGAAGG TCAGCCGAGG TCGGGGTGTT 
CGAGAGTACA ATCCCCCTGA AGATCTTAGG GGCAAGGCCT CCCGGAGGAG GATTGCAAGC 
GAAACACACA GTGAGGGGTC AGAATATGAA GAGCTTCCGA AACGCAGGAG GCAGAGAGGG 
ACGGAGAATG GCAATGATCC TGCTGTACAG GAGAGGGAAG CTGAAGATCT GCGAAAGGGG 
GACTTTCAGG ATTCATGGAG ATCTAATAGG AATTATTCAG ACGATCAGAA TAACACTGAC 
TCAAAATCAA GAGCTCCAAG AGCTTTTGGA AGGTCCCTTC CGCCAAGGTT GAGCAACAGC 
TATGGTAGGA GACCATTATC CACCAAGGAG TCCTCTCACT GGCAAGCCAA GTCTGGGGGA 
TCGTCTTGGC AAGAGTATGG GGCGCCAGCT GAAGCCTATG GGAGTCGCCA TCATGCAGAC 
AGAGACTATG CCCATGACTA TAGATACGCA GACTCATTTC CTAGTAGGGG TTTTGATGAA 
AGTCACGGAG ATGAGAGGAG GTCTTTTTTC CAAGAGGAAT ATTCAGACCG GGATAGTTTG 
GAAAAGAGAT CTTTTGGCAG GCGAAGGCCC CCACGCCAAG ACAAACCTCC GAGGTTTAGG 
CGTCTTAGAC AGGAAAGAGA CCCTTTGGGA CAGTGGAGTG GTGAAGAGAC AGTCGGCACT 
GGAAACAGCA GTGAACACTG GCAGTCTCGT CCTAGAGTCC CACTAAATGA CAAATCTGGG 
CCGGTTCATA GGAGGTCTCC AGAAATGTCC CACCACAACT CTGATCATGT CACCGAAGAC 
TGGGAAACTG CTTCAGAAAG CAGCGACTTC AGTGAAAAGA GGCCGGGGGA TATGGATGGA 
GATGGGAGCC AGAGTGGCAG TGGGCTGTCT GAAAAACGAG AACTGTCCAA GAGAAGTTTC 
TCCAGCCAGA GACCTCTAAT GGATCGTCAG AGTCGTAAAG TGGAGCCATC AGGTTATGAT 
GAGAAGCCTT CACGGGTCAG TGGGAGTTCC TCCCGCAATG ACTTCCAGAA GAGTTCTGGT 
CCTTTAAAGA GCAGCCGCTG CTCTGACGAT TCCTATGGTG CAGAGAGCAG CCACCACCGT 
TACGCCATGG ACAGGTCTAT GCACTGCGAC AGCGGAGAGG CGGGGAAGAA ACAGGAAAGG 
GATTCAAGGA CGCCTGGTCT CAAATCCAGT GATAAAAATG AGGTGATCGG ACAGTTTGAC 
CTCAAATATG GAGATTCCAT GCTTGAGGAA GAGTCCGAGG TCGGAGACAT TGGTAACAAG 
CCTCGTCGTG TGTTGGAAAA GGATCGCCGG AAAAAGGACC AGGTGGTTCA GGTGCCGGCA 
AAGGGCAGCA GTATCCAGTC TAGGATTCCC CCTCGCTTTG CCAAAAAACA GAACGGCATG 
TGCCTGGATC AAGCCGACGT TACTGCCAAA GAAATCTGGG AGAGCAGCGG CCAAGGAATC 
TCTGTCCAGC CTGGCACGGA TACCTGGAGT AAACCTGTGA GCTCATTTAG CACGGAGTCC 
AGCTCCACAG AGGGCTTTAA AGGCAGTCAA GGAGACAGTG GCATTGACCT GAGTGCCGAG 
TCTCGGGAGT CGTCTGCTAC ATCTTCACAA CGCAGCTCTC CGTATGGAAC CATGAAACCG 
GAGGAAATGA ACGGAACAGG TGTGGCAGAG CCAAAACCAG ATTGCCCTAA AGAACAAGGG 
CAAAAACAGT CTGAGAAAAA GGATTCCGAC CCTGGCTCGG GCCTAAATAA AGAACATAAA 
CCTGGACCCA TTGGCAACGA ACGCTCGCTG AAAAACCGAA AAGGATCCGA GGGCACCGAA 
CGGTTGGAAG GCGGCGTCCC CCCAGTCAAT GGAGTAGAGA TCCACGTGGA TTCGGTTCTC 
CCTGTTCCGC CTATTGAATT TGGAGTAAGC GCAAAAGATG CAGACTATGC CCTCCCTCCT 
GGTACTGCCA CCGTTCAAGC AGCCAATACT GTTACAAAGT TGCAGGATGC TTTGGCCAGT 
AAGGCAGGGC TAACGCAGTC CATCCCCATC CTAAGGAGAG ACCACCACAT GCAGCAGGGT 
ATGAGCCTAA ACATGTCCTA CCCCACTGCG GATCTCACCC TTAAGATGGA GTCTGCCCGC 
AAAGCCTGGG AAAACTCGCC CAGTTTGCCA GAGCAGAATT CGCCAGCAGG CCCTGGCTCA 
GGAATTCAGC CGCCATCTAG TGTGGGAGCC TCCACCGGGG TCAACTACAG CTCCTTTGGC 
GGCGTGTCAA TGCCACCCAT GCCTGTTGCC TCTGTCGCCC CGTCTGCCTC CATGCCAGGA 
AACCACATCC CACCCCTGTA CCTTGATGGG CACGTGTTTA CCAACCAGCC GCGCCTGGTG 
CAGCAAACCA TACCTCAACA GCAAGGGTAT CAGCAGGCAG CAGCTGCCCA GCAGATCCCC 
ATTTCCCTGC ACACCTCCCT GCAGGCTCAA GCCCAGCTGG GTTTGCGAGG AGGCCTCCCC 
GTGTCCCAGT CGCAAGAAAT TTACAGCTCC ATGCAGCCGT TCAGGTCCCA GGTGTACATG 
CATCCAAGTC TTTCTCAGCC CAGTGCTATG GTACTGACCA GTGGCACAGG CCTCAAGCCC 
CCATACAACC CTTTCCCTGG CATGCAGACA CTGGAAATGG TTAAAACCCA GCCCACGTCC 
CCTTATCAGC CCCTGAATGG GAGCCAGCAG CTGGTGTATG AAAGCCAACT GAACCAGGCC 
TCCCAGATGA TGGACTCCCA GCTCACACAG TTGACCATGC CCATGCCCGG CTCCCAGCTC 
CAGATGCCCC GATACAGTTC AGGACAGCAA ACAATGATCT TACCACAGTC CATACAGCTT 
CCCCAGGGGC AGAACCTGCC TGTAGGGGCT CCTCGGAGAA TGCAGCCTTC TATGCTGACA 
AGCAGAGAGT CCTCCCAGAT GGAGATGAAA AGCTTCCACT TTACAGACGG CAAACAGAAC 
ATGCCGACTG CCATGCAAGC ACAGCACTCC TATCGGCCGA GTTCAGCTAG CCCCAGTGGA 
AAGTCGCCCG GACCGGGGCC ATCTGCCAAC TTGGGCTCTG TGCAGGGACA CTACCCTCAA 
CAGGTAAAAC ACAGATCCGA CGACAGCAAA GGCGGCCTGA GGTCCCCCAA AGTTCCCGAG 
CAGCCCTCAG CGAGTCCCAT GAAGCCGGCC AGGACGGGGG CCATCAAGCC TCAAGCGGTG 
AAAGTGGAAG AGAGCAAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824435.2
CDS	319..6822
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQNVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKHRSDDSKGGLRSPKVPEQPSASPMKPARTGAIKPQAVKVEESKA

Target ORF information:

RefSeq Version	XM_012968981.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968981.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAATGTCAC CAGTTGGAGA GACGGCGGTG GGAGAAGCAT CGCTTCCAGC 
TTATCTCCGA CCGCCTCTCC TACTGAGCCG GGCAGCAAAA CGGCAGTACC GGGAGAAGGG 
CCCCCCTCTA CATCGCTTGC TAATGATACT AAAGAACTCT CGCTGCGCCC GGCTCAGCCC 
ACCCGAAAAG GCCCTGCTCA GTTCATGGGA AACACCTACC ACCCACCGAC TTACCATGAC 
ATGCTACCAG CCTTTATGTG CTCTCAGCAC CCACCAGAGC CTTCTGGGAC ATTGGATCGT 
ACTTCCTTTC CCACCCTTAA TTCTCAGTCT CGCCTTGAAC CTCGAGTCCC TTTTCGCCAA 
TTTCAGATGA ATGATAACGA AGGACGGGAG AGCAGGTTTC CCATTGCACC CCCTCGTTCT 
GCACGACCCC CCCGCCCACC AGCCGAGCGC CCCCCAAGAG CCACCATCAT CAATGCAGAG 
GATCTAAAGG AGCTGGATGA CCTTGATAAT GATGCAGAGG ATGGCTGGGC TGGTCTGCAC 
GATGAGGTTG ATTATTCCGA GAAACTGAAG TTCAGTGAAG ATGAAGAAGA GGAGGACGGT 
GCTGCAAAAG ATGGCAGAAG TAAATGGAAT GGTTGGGATG GCAAAAGGCA GCGGCAGGTT 
TCCTTCAATT CTACTGATAG CGTGGAGGGA AAACATCCAG CAGATGAAGG AAAGGTGTGG 
AACGACACAT TGTCCCATTC GCGTCCTGCA AGAAGAACAC CGGAACCTGC GCCACAAGGG 
CCACGTAAAC CCAACACTTG GGCTGCTGTG GCTGATCATC AGAAACCCCC TGCCCCTGCT 
GCAGTCCTGC GACAGCCCTC CATAGAAGAG AAAGATGAAA AGCTGCCCCA GAGGAAAAAT 
TTTGTCCAGT CCGAGATCTC TGAGGCAGTA GAGCGTGCCC GCAAAAGGAG AGAGGAGGAG 
GAGAGGCGAA ACCGCGAGGA ACGGCTGGCT GCTTGTGCTG CTAAACTAAA ACAGCTTGAT 
CAGAAGAACA AGCAGGCCCT GAAACCATCG GCAGATTCTT CCCGCCAGCT GGACAATAAG 
GAGAATGAAG ACCCCCGTTC TCCTGTGTCA GACAGAAGCA ACCCCCAGGA AAACGCACAT 
ACTTTCAGAA GAGAATACTC TCAGGAGTCC CCTTCTGAAT ATCCAGAGGA GGAGCCTTCT 
GGAACCCTAA AAGCTGAAAC CAGCAGCGAA GAAGAATATG CGGAACCTAC ATCCCCAGCA 
CAAGATTTCA GCAAACATCA GAAACTGATT CCACCCCGAT TCCAGCGGCA GCAACAGCAG 
CAGGAGCAGT TGTACAAGAT GCAGCAGTGG CAGCAGCAGC AACAGCAAGC CTATGTGGCC 
TCCACTCATT CCAACCCTCC CCGGACCTTC TACTCCCCTC ACCCACAGAT GTTGGGCTTT 
GATCCACGCT GGATGATGAT GCCTTCTTAC ATGGACCCAC GGATGACGCA AGGCCGTGCG 
CCTGTGGACT TCTACCCTTC CTCCATCCAC CCTCCAGGTG TAATGAAGCA TATGATCCAG 
CAGGATTCAA TGAGCGGTTC TGGAAGTTGC CATTCAGACG AACAGAACTG CCAGTCTGAG 
AGGAGACCTC AGTCAGCAGA ACCGATGTCT GGTTGGGGCC AGGAAAGCTA CGTGCAATTA 
CACAGCAAGG CCTGCTCCCT GCCTCAGCAG AAACAGGCTG AGACTGCCGC AGTGGGGAGC 
TACAGTAGGA ATGAAAGTTC TTACTCATCC GCAAGAGAGA AATCAGATGG CTCCCCTACC 
CACCCTGAGC CCTTAGAGAG GGAAGAGGAG TATTTATCTG TTTCTTATGA AAAGAAATCC 
ACTGGGAGCT TTAGCAGTTG CATTTCCCCT CAAAGGAGAG GACAGGACGC CTTGTACCAA 
CATCTAGAGA GCGGCATAGA GTCTGGTGTG AACAGGGGGA GACAGATGGC AGCCAGGGAT 
TCGTCCTCAA GAGGCTCTGA GTTTATAAAG ACTGATAAAA AGCCACAGTT TAATGGCTGG 
GGTTATGCAC CCCATCAGAA ATCTTCCGAA ACAGCCAGCA GAGCAGAAGA GGATCTTTCA 
AAGGAGGACC TATCTGATGA ACTGGAGCAC TGGAAGAAGG AGGATGGTGG GAGCAGCCAG 
GATGGTACAG GAGAAACCTG CTGGAGAAGT GAATCTGCAA ATTCCAACAG TCAGCAGCAA 
TCTGAGCATC TAGGGAGAAG CCGTAGATCT GGACCTATCA AAAAGCCCGT CTTGAAAGCC 
CTCAAGATAG AGGACAAAGA GCTGGAGAAA AACAAGGCAG AAATTAAAGA GCCAGTAAAA 
CCTTTTAAGG AAAAAATTCT TCCAAAAACA GAGTGCGTTG TTAAGACTGA ACCTGTTGTT 
TCAAGCCCTC CTCCCAGCAT GATTGAAGTT AAAACTCCCC CTCCGCCTAG TCCTGTCCAG 
GAGACTGATA AATTCCCCCA AGAAAAAGCT GAAAGGAGTT GGGAGACCAA GCCTTTACGG 
GAATCTAGTA GCACTTCTTT GCAAAAGAGA AGTGACTGGA TATTCATTGA CGAAGAGCAA 
GCGTTTGGCG GTAGGACTCA AGGCCGTGGC AGAGGTAGAG GGTTTAAAGA CTTTGGTTTC 
CGCAACCGAG GGGTCTCTGG CACTTACAGC GGGCAGAAGG TCAGCCGAGG TCGGGGTGTT 
CGAGAGTACA ATCCCCCTGA AGATCTTAGG GGCAAGGCCT CCCGGAGGAG GATTGCAAGC 
GAAACACACA GTGAGGGGTC AGAATATGAA GAGCTTCCGA AACGCAGGAG GCAGAGAGGG 
ACGGAGAATG GCAATGATCC TGCTGTACAG GAGAGGGAAG CTGAAGATCT GCGAAAGGGG 
GACTTTCAGG ATTCATGGAG ATCTAATAGG AATTATTCAG ACGATCAGAA TAACACTGAC 
TCAAAATCAA GAGCTCCAAG AGCTTTTGGA AGGTCCCTTC CGCCAAGGTT GAGCAACAGC 
TATGGTAGGA GACCATTATC CACCAAGGAG TCCTCTCACT GGCAAGCCAA GTCTGGGGGA 
TCGTCTTGGC AAGAGTATGG GGCGCCAGCT GAAGCCTATG GGAGTCGCCA TCATGCAGAC 
AGAGACTATG CCCATGACTA TAGATACGCA GACTCATTTC CTAGTAGGGG TTTTGATGAA 
AGTCACGGAG ATGAGAGGAG GTCTTTTTTC CAAGAGGAAT ATTCAGACCG GGATAGTTTG 
GAAAAGAGAT CTTTTGGCAG GCGAAGGCCC CCACGCCAAG ACAAACCTCC GAGGTTTAGG 
CGTCTTAGAC AGGAAAGAGA CCCTTTGGGA CAGTGGAGTG GTGAAGAGAC AGTCGGCACT 
GGAAACAGCA GTGAACACTG GCAGTCTCGT CCTAGAGTCC CACTAAATGA CAAATCTGGG 
CCGGTTCATA GGAGGTCTCC AGAAATGTCC CACCACAACT CTGATCATGT CACCGAAGAC 
TGGGAAACTG CTTCAGAAAG CAGCGACTTC AGTGAAAAGA GGCCGGGGGA TATGGATGGA 
GATGGGAGCC AGAGTGGCAG TGGGCTGTCT GAAAAACGAG AACTGTCCAA GAGAAGTTTC 
TCCAGCCAGA GACCTCTAAT GGATCGTCAG AGTCGTAAAG TGGAGCCATC AGGTTATGAT 
GAGAAGCCTT CACGGGTCAG TGGGAGTTCC TCCCGCAATG ACTTCCAGAA GAGTTCTGGT 
CCTTTAAAGA GCAGCCGCTG CTCTGACGAT TCCTATGGTG CAGAGAGCAG CCACCACCGT 
TACGCCATGG ACAGGTCTAT GCACTGCGAC AGCGGAGAGG CGGGGAAGAA ACAGGAAAGG 
GATTCAAGGA CGCCTGGTCT CAAATCCAGT GATAAAAATG AGGTGATCGG ACAGTTTGAC 
CTCAAATATG GAGATTCCAT GCTTGAGGAA GAGTCCGAGG TCGGAGACAT TGGTAACAAG 
CCTCGTCGTG TGTTGGAAAA GGATCGCCGG AAAAAGGACC AGGTGGTTCA GGTGCCGGCA 
AAGGGCAGCA GTATCCAGTC TAGGATTCCC CCTCGCTTTG CCAAAAAACA GAACGGCATG 
TGCCTGGATC AAGCCGACGT TACTGCCAAA GAAATCTGGG AGAGCAGCGG CCAAGGAATC 
TCTGTCCAGC CTGGCACGGA TACCTGGAGT AAACCTGTGA GCTCATTTAG CACGGAGTCC 
AGCTCCACAG AGGGCTTTAA AGGCAGTCAA GGAGACAGTG GCATTGACCT GAGTGCCGAG 
TCTCGGGAGT CGTCTGCTAC ATCTTCACAA CGCAGCTCTC CGTATGGAAC CATGAAACCG 
GAGGAAATGA ACGGAACAGG TGTGGCAGAG CCAAAACCAG ATTGCCCTAA AGAACAAGGG 
CAAAAACAGT CTGAGAAAAA GGATTCCGAC CCTGGCTCGG GCCTAAATAA AGAACATAAA 
CCTGGACCCA TTGGCAACGA ACGCTCGCTG AAAAACCGAA AAGGATCCGA GGGCACCGAA 
CGGTTGGAAG GCGGCGTCCC CCCAGTCAAT GGAGTAGAGA TCCACGTGGA TTCGGTTCTC 
CCTGTTCCGC CTATTGAATT TGGAGTAAGC GCAAAAGATG CAGACTATGC CCTCCCTCCT 
GGTACTGCCA CCGTTCAAGC AGCCAATACT GTTACAAAGT TGCAGGATGC TTTGGCCAGT 
AAGGCAGGGC TAACGCAGTC CATCCCCATC CTAAGGAGAG ACCACCACAT GCAGCAGGGT 
ATGAGCCTAA ACATGTCCTA CCCCACTGCG GATCTCACCC TTAAGATGGA GTCTGCCCGC 
AAAGCCTGGG AAAACTCGCC CAGTTTGCCA GAGCAGAATT CGCCAGCAGG CCCTGGCTCA 
GGAATTCAGC CGCCATCTAG TGTGGGAGCC TCCACCGGGG TCAACTACAG CTCCTTTGGC 
GGCGTGTCAA TGCCACCCAT GCCTGTTGCC TCTGTCGCCC CGTCTGCCTC CATGCCAGGA 
AACCACATCC CACCCCTGTA CCTTGATGGG CACGTGTTTA CCAACCAGCC GCGCCTGGTG 
CAGCAAACCA TACCTCAACA GCAAGGGTAT CAGCAGGCAG CAGCTGCCCA GCAGATCCCC 
ATTTCCCTGC ACACCTCCCT GCAGGCTCAA GCCCAGCTGG GTTTGCGAGG AGGCCTCCCC 
GTGTCCCAGT CGCAAGAAAT TTACAGCTCC ATGCAGCCGT TCAGGTCCCA GGTGTACATG 
CATCCAAGTC TTTCTCAGCC CAGTGCTATG GTACTGACCA GTGGCACAGG CCTCAAGCCC 
CCATACAACC CTTTCCCTGG CATGCAGACA CTGGAAATGG TTAAAACCCA GCCCACGTCC 
CCTTATCAGC CCCTGAATGG GAGCCAGCAG CTGGTGTATG AAAGCCAACT GAACCAGGCC 
TCCCAGATGA TGGACTCCCA GCTCACACAG TTGACCATGC CCATGCCCGG CTCCCAGCTC 
CAGATGCCCC GATACAGTTC AGGACAGCAA ACAATGATCT TACCACAGTC CATACAGCTT 
CCCCAGGGGC AGAACCTGCC TGTAGGGGCT CCTCGGAGAA TGCAGCCTTC TATGCTGACA 
AGCAGAGAGT CCTCCCAGAT GGAGATGAAA AGCTTCCACT TTACAGACGG CAAACAGAAC 
ATGCCGACTG CCATGCAAGC ACAGCACTCC TATCGGCCGA GTTCAGCTAG CCCCAGTGGA 
AAGTCGCCCG GACCGGGGCC ATCTGCCAAC TTGGGCTCTG TGCAGGGACA CTACCCTCAA 
CAGGTAAAAC ACAGATCCGA CGACAGCAAA GGCGGCCTGA GGTCCCCCAA AGTTCCCGAG 
CAGCCCTCAG CGAGTCCCAT GAAGCCGGCC AGGACGGGGG CCATCAAGCC TCAAGCGGTG 
AAAGTGGAAG AGAGCAAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968983.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824437.2 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

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CloneID	OXa64141
Clone ID Related Accession (Same CDS sequence)	XM_012968983.3
Accession Version	XM_012968983.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968983.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GCAGGGCTAA CGCAGTCCAT CCCCATCCTA AGGAGAGACC ACCACATGCA GCAGGGTATG 
AGCCTAAACA TGTCCTACCC CACTGCGGAT CTCACCCTTA AGATGGAGTC TGCCCGCAAA 
GCCTGGGAAA ACTCGCCCAG TTTGCCAGAG CAGAATTCGC CAGCAGGCCC TGGCTCAGGA 
ATTCAGCCGC CATCTAGTGT GGGAGCCTCC ACCGGGGTCA ACTACAGCTC CTTTGGCGGC 
GTGTCAATGC CACCCATGCC TGTTGCCTCT GTCGCCCCGT CTGCCTCCAT GCCAGGAAAC 
CACATCCCAC CCCTGTACCT TGATGGGCAC GTGTTTACCA ACCAGCCGCG CCTGGTGCAG 
CAAACCATAC CTCAACAGCA AGGGTATCAG CAGGCAGCAG CTGCCCAGCA GATCCCCATT 
TCCCTGCACA CCTCCCTGCA GGCTCAAGCC CAGCTGGGTT TGCGAGGAGG CCTCCCCGTG 
TCCCAGTCGC AAGAAATTTA CAGCTCCATG CAGCCGTTCA GGTCCCAGGT GTACATGCAT 
CCAAGTCTTT CTCAGCCCAG TGCTATGGTA CTGACCAGTG GCACAGGCCT CAAGCCCCCA 
TACAACCCTT TCCCTGGCAT GCAGACACTG GAAATGGTTA AAACCCAGCC CACGTCCCCT 
TATCAGCCCC TGAATGGGAG CCAGCAGCTG GTGTATGAAA GCCAACTGAA CCAGGCCTCC 
CAGATGATGG ACTCCCAGCT CACACAGTTG ACCATGCCCA TGCCCGGCTC CCAGCTCCAG 
ATGCCCCGAT ACAGTTCAGG ACAGCAAACA ATGATCTTAC CACAGTCCAT ACAGCTTCCC 
CAGGGGCAGA ACCTGCCTGT AGGGGCTCCT CGGAGAATGC AGCCTTCTAT GCTGACAAGC 
AGAGAGTCCT CCCAGATGGA GATGAAAAGC TTCCACTTTA CAGACGGCAA ACAGAACATG 
CCGACTGCCA TGCAAGCACA GCACTCCTAT CGGCCGAGTT CAGCTAGCCC CAGTGGAAAG 
TCGCCCGGAC CGGGGCCATC TGCCAACTTG GGCTCTGTGC AGGGACACTA CCCTCAACAG 
GTAAAACACA GATCCGACGA CAGCAAAGGC GGCCTGAGGT CCCCCAAAGT TCCCGAGCAG 
CCCTCAGCGA GTCCCATGAA GCCGGCCAGG ACGGGGGCCA TCAAGCCTCA AGCGGTGAAA 
GTGGAAGAGA GCAAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824437.2
CDS	319..6759
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKHRSDDSKGGLRSPKVPEQPSASPMKPARTGAIKPQAVKVEESKA

Target ORF information:

RefSeq Version	XM_012968983.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968983.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GCAGGGCTAA CGCAGTCCAT CCCCATCCTA AGGAGAGACC ACCACATGCA GCAGGGTATG 
AGCCTAAACA TGTCCTACCC CACTGCGGAT CTCACCCTTA AGATGGAGTC TGCCCGCAAA 
GCCTGGGAAA ACTCGCCCAG TTTGCCAGAG CAGAATTCGC CAGCAGGCCC TGGCTCAGGA 
ATTCAGCCGC CATCTAGTGT GGGAGCCTCC ACCGGGGTCA ACTACAGCTC CTTTGGCGGC 
GTGTCAATGC CACCCATGCC TGTTGCCTCT GTCGCCCCGT CTGCCTCCAT GCCAGGAAAC 
CACATCCCAC CCCTGTACCT TGATGGGCAC GTGTTTACCA ACCAGCCGCG CCTGGTGCAG 
CAAACCATAC CTCAACAGCA AGGGTATCAG CAGGCAGCAG CTGCCCAGCA GATCCCCATT 
TCCCTGCACA CCTCCCTGCA GGCTCAAGCC CAGCTGGGTT TGCGAGGAGG CCTCCCCGTG 
TCCCAGTCGC AAGAAATTTA CAGCTCCATG CAGCCGTTCA GGTCCCAGGT GTACATGCAT 
CCAAGTCTTT CTCAGCCCAG TGCTATGGTA CTGACCAGTG GCACAGGCCT CAAGCCCCCA 
TACAACCCTT TCCCTGGCAT GCAGACACTG GAAATGGTTA AAACCCAGCC CACGTCCCCT 
TATCAGCCCC TGAATGGGAG CCAGCAGCTG GTGTATGAAA GCCAACTGAA CCAGGCCTCC 
CAGATGATGG ACTCCCAGCT CACACAGTTG ACCATGCCCA TGCCCGGCTC CCAGCTCCAG 
ATGCCCCGAT ACAGTTCAGG ACAGCAAACA ATGATCTTAC CACAGTCCAT ACAGCTTCCC 
CAGGGGCAGA ACCTGCCTGT AGGGGCTCCT CGGAGAATGC AGCCTTCTAT GCTGACAAGC 
AGAGAGTCCT CCCAGATGGA GATGAAAAGC TTCCACTTTA CAGACGGCAA ACAGAACATG 
CCGACTGCCA TGCAAGCACA GCACTCCTAT CGGCCGAGTT CAGCTAGCCC CAGTGGAAAG 
TCGCCCGGAC CGGGGCCATC TGCCAACTTG GGCTCTGTGC AGGGACACTA CCCTCAACAG 
GTAAAACACA GATCCGACGA CAGCAAAGGC GGCCTGAGGT CCCCCAAAGT TCCCGAGCAG 
CCCTCAGCGA GTCCCATGAA GCCGGCCAGG ACGGGGGCCA TCAAGCCTCA AGCGGTGAAA 
GTGGAAGAGA GCAAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968979.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824433.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

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CloneID	OXa35271
Clone ID Related Accession (Same CDS sequence)	XM_012968979.2
Accession Version	XM_012968979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
CCGAGTTCAG CTAGCCCCAG TGGAAAGTCG CCCGGACCGG GGCCATCTGC CAACTTGGGC 
TCTGTGCAGG GACACTACCC TCAACAGGTA AAGACCAGTC CTGTCACAAA GAGCGAACCC 
ATATTGTACC ATCTCGTTCA TGTTCATGGC ATCCTAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824433.1
CDS	319..6780
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKTSPVTKSEPILYHLVHVHGILV

Target ORF information:

RefSeq Version	XM_012968979.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968979.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
CCGAGTTCAG CTAGCCCCAG TGGAAAGTCG CCCGGACCGG GGCCATCTGC CAACTTGGGC 
TCTGTGCAGG GACACTACCC TCAACAGGTA AAGACCAGTC CTGTCACAAA GAGCGAACCC 
ATATTGTACC ATCTCGTTCA TGTTCATGGC ATCCTAGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968980.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824434.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	OXa35272
Clone ID Related Accession (Same CDS sequence)	XM_012968980.2
Accession Version	XM_012968980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGA TGCAGACTAT 
GCCCTCCCTC CTGGTACTGC CACCGTTCAA GCAGCCAATA CTGTTACAAA GTTGCAGGAT 
GCTTTGGCCA GTAAGGCAGG GCTAACGCAG TCCATCCCCA TCCTAAGGAG AGACCACCAC 
ATGCAGCAGG GTATGAGCCT AAACATGTCC TACCCCACTG CGGATCTCAC CCTTAAGATG 
GAGTCTGCCC GCAAAGCCTG GGAAAACTCG CCCAGTTTGC CAGAGCAGAA TTCGCCAGCA 
GGCCCTGGCT CAGGAATTCA GCCGCCATCT AGTGTGGGAG CCTCCACCGG GGTCAACTAC 
AGCTCCTTTG GCGGCGTGTC AATGCCACCC ATGCCTGTTG CCTCTGTCGC CCCGTCTGCC 
TCCATGCCAG GAAACCACAT CCCACCCCTG TACCTTGATG GGCACGTGTT TACCAACCAG 
CCGCGCCTGG TGCAGCAAAC CATACCTCAA CAGCAAGGGT ATCAGCAGGC AGCAGCTGCC 
CAGCAGATCC CCATTTCCCT GCACACCTCC CTGCAGGCTC AAGCCCAGCT GGGTTTGCGA 
GGAGGCCTCC CCGTGTCCCA GTCGCAAGAA ATTTACAGCT CCATGCAGCC GTTCAGGTCC 
CAGGTGTACA TGCATCCAAG TCTTTCTCAG CCCAGTGCTA TGGTACTGAC CAGTGGCACA 
GGCCTCAAGC CCCCATACAA CCCTTTCCCT GGCATGCAGA CACTGGAAAT GGTTAAAACC 
CAGCCCACGT CCCCTTATCA GCCCCTGAAT GGGAGCCAGC AGCTGGTGTA TGAAAGCCAA 
CTGAACCAGG CCTCCCAGAT GATGGACTCC CAGCTCACAC AGTTGACCAT GCCCATGCCC 
GGCTCCCAGC TCCAGATGCC CCGATACAGT TCAGGACAGC AAACAATGAT CTTACCACAG 
TCCATACAGC TTCCCCAGGG GCAGAACCTG CCTGTAGGGG CTCCTCGGAG AATGCAGCCT 
TCTATGCTGA CAAGCAGAGA GTCCTCCCAG ATGGAGATGA AAAGCTTCCA CTTTACAGAC 
GGCAAACAGA ACATGCCGAC TGCCATGCAA GCACAGCACT CCTATCGGCC GAGTTCAGCT 
AGCCCCAGTG GAAAGTCGCC CGGACCGGGG CCATCTGCCA ACTTGGGCTC TGTGCAGGGA 
CACTACCCTC AACAGGTAAA GACCAGTCCT GTCACAAAGA GCGAACCCAT ATTGTACCAT 
CTCGTTCATG TTCATGGCAT CCTAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824434.1
CDS	319..6768
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKTSPVTKSEPILYHLVHVHGILV

Target ORF information:

RefSeq Version	XM_012968980.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968980.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGA TGCAGACTAT 
GCCCTCCCTC CTGGTACTGC CACCGTTCAA GCAGCCAATA CTGTTACAAA GTTGCAGGAT 
GCTTTGGCCA GTAAGGCAGG GCTAACGCAG TCCATCCCCA TCCTAAGGAG AGACCACCAC 
ATGCAGCAGG GTATGAGCCT AAACATGTCC TACCCCACTG CGGATCTCAC CCTTAAGATG 
GAGTCTGCCC GCAAAGCCTG GGAAAACTCG CCCAGTTTGC CAGAGCAGAA TTCGCCAGCA 
GGCCCTGGCT CAGGAATTCA GCCGCCATCT AGTGTGGGAG CCTCCACCGG GGTCAACTAC 
AGCTCCTTTG GCGGCGTGTC AATGCCACCC ATGCCTGTTG CCTCTGTCGC CCCGTCTGCC 
TCCATGCCAG GAAACCACAT CCCACCCCTG TACCTTGATG GGCACGTGTT TACCAACCAG 
CCGCGCCTGG TGCAGCAAAC CATACCTCAA CAGCAAGGGT ATCAGCAGGC AGCAGCTGCC 
CAGCAGATCC CCATTTCCCT GCACACCTCC CTGCAGGCTC AAGCCCAGCT GGGTTTGCGA 
GGAGGCCTCC CCGTGTCCCA GTCGCAAGAA ATTTACAGCT CCATGCAGCC GTTCAGGTCC 
CAGGTGTACA TGCATCCAAG TCTTTCTCAG CCCAGTGCTA TGGTACTGAC CAGTGGCACA 
GGCCTCAAGC CCCCATACAA CCCTTTCCCT GGCATGCAGA CACTGGAAAT GGTTAAAACC 
CAGCCCACGT CCCCTTATCA GCCCCTGAAT GGGAGCCAGC AGCTGGTGTA TGAAAGCCAA 
CTGAACCAGG CCTCCCAGAT GATGGACTCC CAGCTCACAC AGTTGACCAT GCCCATGCCC 
GGCTCCCAGC TCCAGATGCC CCGATACAGT TCAGGACAGC AAACAATGAT CTTACCACAG 
TCCATACAGC TTCCCCAGGG GCAGAACCTG CCTGTAGGGG CTCCTCGGAG AATGCAGCCT 
TCTATGCTGA CAAGCAGAGA GTCCTCCCAG ATGGAGATGA AAAGCTTCCA CTTTACAGAC 
GGCAAACAGA ACATGCCGAC TGCCATGCAA GCACAGCACT CCTATCGGCC GAGTTCAGCT 
AGCCCCAGTG GAAAGTCGCC CGGACCGGGG CCATCTGCCA ACTTGGGCTC TGTGCAGGGA 
CACTACCCTC AACAGGTAAA GACCAGTCCT GTCACAAAGA GCGAACCCAT ATTGTACCAT 
CTCGTTCATG TTCATGGCAT CCTAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968981.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824435.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

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CloneID	OXa35273
Clone ID Related Accession (Same CDS sequence)	XM_012968981.2
Accession Version	XM_012968981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAATGTCAC CAGTTGGAGA GACGGCGGTG GGAGAAGCAT CGCTTCCAGC 
TTATCTCCGA CCGCCTCTCC TACTGAGCCG GGCAGCAAAA CGGCAGTACC GGGAGAAGGG 
CCCCCCTCTA CATCGCTTGC TAATGATACT AAAGAACTCT CGCTGCGCCC GGCTCAGCCC 
ACCCGAAAAG GCCCTGCTCA GTTCATGGGA AACACCTACC ACCCACCGAC TTACCATGAC 
ATGCTACCAG CCTTTATGTG CTCTCAGCAC CCACCAGAGC CTTCTGGGAC ATTGGATCGT 
ACTTCCTTTC CCACCCTTAA TTCTCAGTCT CGCCTTGAAC CTCGAGTCCC TTTTCGCCAA 
TTTCAGATGA ATGATAACGA AGGACGGGAG AGCAGGTTTC CCATTGCACC CCCTCGTTCT 
GCACGACCCC CCCGCCCACC AGCCGAGCGC CCCCCAAGAG CCACCATCAT CAATGCAGAG 
GATCTAAAGG AGCTGGATGA CCTTGATAAT GATGCAGAGG ATGGCTGGGC TGGTCTGCAC 
GATGAGGTTG ATTATTCCGA GAAACTGAAG TTCAGTGAAG ATGAAGAAGA GGAGGACGGT 
GCTGCAAAAG ATGGCAGAAG TAAATGGAAT GGTTGGGATG GCAAAAGGCA GCGGCAGGTT 
TCCTTCAATT CTACTGATAG CGTGGAGGGA AAACATCCAG CAGATGAAGG AAAGGTGTGG 
AACGACACAT TGTCCCATTC GCGTCCTGCA AGAAGAACAC CGGAACCTGC GCCACAAGGG 
CCACGTAAAC CCAACACTTG GGCTGCTGTG GCTGATCATC AGAAACCCCC TGCCCCTGCT 
GCAGTCCTGC GACAGCCCTC CATAGAAGAG AAAGATGAAA AGCTGCCCCA GAGGAAAAAT 
TTTGTCCAGT CCGAGATCTC TGAGGCAGTA GAGCGTGCCC GCAAAAGGAG AGAGGAGGAG 
GAGAGGCGAA ACCGCGAGGA ACGGCTGGCT GCTTGTGCTG CTAAACTAAA ACAGCTTGAT 
CAGAAGAACA AGCAGGCCCT GAAACCATCG GCAGATTCTT CCCGCCAGCT GGACAATAAG 
GAGAATGAAG ACCCCCGTTC TCCTGTGTCA GACAGAAGCA ACCCCCAGGA AAACGCACAT 
ACTTTCAGAA GAGAATACTC TCAGGAGTCC CCTTCTGAAT ATCCAGAGGA GGAGCCTTCT 
GGAACCCTAA AAGCTGAAAC CAGCAGCGAA GAAGAATATG CGGAACCTAC ATCCCCAGCA 
CAAGATTTCA GCAAACATCA GAAACTGATT CCACCCCGAT TCCAGCGGCA GCAACAGCAG 
CAGGAGCAGT TGTACAAGAT GCAGCAGTGG CAGCAGCAGC AACAGCAAGC CTATGTGGCC 
TCCACTCATT CCAACCCTCC CCGGACCTTC TACTCCCCTC ACCCACAGAT GTTGGGCTTT 
GATCCACGCT GGATGATGAT GCCTTCTTAC ATGGACCCAC GGATGACGCA AGGCCGTGCG 
CCTGTGGACT TCTACCCTTC CTCCATCCAC CCTCCAGGTG TAATGAAGCA TATGATCCAG 
CAGGATTCAA TGAGCGGTTC TGGAAGTTGC CATTCAGACG AACAGAACTG CCAGTCTGAG 
AGGAGACCTC AGTCAGCAGA ACCGATGTCT GGTTGGGGCC AGGAAAGCTA CGTGCAATTA 
CACAGCAAGG CCTGCTCCCT GCCTCAGCAG AAACAGGCTG AGACTGCCGC AGTGGGGAGC 
TACAGTAGGA ATGAAAGTTC TTACTCATCC GCAAGAGAGA AATCAGATGG CTCCCCTACC 
CACCCTGAGC CCTTAGAGAG GGAAGAGGAG TATTTATCTG TTTCTTATGA AAAGAAATCC 
ACTGGGAGCT TTAGCAGTTG CATTTCCCCT CAAAGGAGAG GACAGGACGC CTTGTACCAA 
CATCTAGAGA GCGGCATAGA GTCTGGTGTG AACAGGGGGA GACAGATGGC AGCCAGGGAT 
TCGTCCTCAA GAGGCTCTGA GTTTATAAAG ACTGATAAAA AGCCACAGTT TAATGGCTGG 
GGTTATGCAC CCCATCAGAA ATCTTCCGAA ACAGCCAGCA GAGCAGAAGA GGATCTTTCA 
AAGGAGGACC TATCTGATGA ACTGGAGCAC TGGAAGAAGG AGGATGGTGG GAGCAGCCAG 
GATGGTACAG GAGAAACCTG CTGGAGAAGT GAATCTGCAA ATTCCAACAG TCAGCAGCAA 
TCTGAGCATC TAGGGAGAAG CCGTAGATCT GGACCTATCA AAAAGCCCGT CTTGAAAGCC 
CTCAAGATAG AGGACAAAGA GCTGGAGAAA AACAAGGCAG AAATTAAAGA GCCAGTAAAA 
CCTTTTAAGG AAAAAATTCT TCCAAAAACA GAGTGCGTTG TTAAGACTGA ACCTGTTGTT 
TCAAGCCCTC CTCCCAGCAT GATTGAAGTT AAAACTCCCC CTCCGCCTAG TCCTGTCCAG 
GAGACTGATA AATTCCCCCA AGAAAAAGCT GAAAGGAGTT GGGAGACCAA GCCTTTACGG 
GAATCTAGTA GCACTTCTTT GCAAAAGAGA AGTGACTGGA TATTCATTGA CGAAGAGCAA 
GCGTTTGGCG GTAGGACTCA AGGCCGTGGC AGAGGTAGAG GGTTTAAAGA CTTTGGTTTC 
CGCAACCGAG GGGTCTCTGG CACTTACAGC GGGCAGAAGG TCAGCCGAGG TCGGGGTGTT 
CGAGAGTACA ATCCCCCTGA AGATCTTAGG GGCAAGGCCT CCCGGAGGAG GATTGCAAGC 
GAAACACACA GTGAGGGGTC AGAATATGAA GAGCTTCCGA AACGCAGGAG GCAGAGAGGG 
ACGGAGAATG GCAATGATCC TGCTGTACAG GAGAGGGAAG CTGAAGATCT GCGAAAGGGG 
GACTTTCAGG ATTCATGGAG ATCTAATAGG AATTATTCAG ACGATCAGAA TAACACTGAC 
TCAAAATCAA GAGCTCCAAG AGCTTTTGGA AGGTCCCTTC CGCCAAGGTT GAGCAACAGC 
TATGGTAGGA GACCATTATC CACCAAGGAG TCCTCTCACT GGCAAGCCAA GTCTGGGGGA 
TCGTCTTGGC AAGAGTATGG GGCGCCAGCT GAAGCCTATG GGAGTCGCCA TCATGCAGAC 
AGAGACTATG CCCATGACTA TAGATACGCA GACTCATTTC CTAGTAGGGG TTTTGATGAA 
AGTCACGGAG ATGAGAGGAG GTCTTTTTTC CAAGAGGAAT ATTCAGACCG GGATAGTTTG 
GAAAAGAGAT CTTTTGGCAG GCGAAGGCCC CCACGCCAAG ACAAACCTCC GAGGTTTAGG 
CGTCTTAGAC AGGAAAGAGA CCCTTTGGGA CAGTGGAGTG GTGAAGAGAC AGTCGGCACT 
GGAAACAGCA GTGAACACTG GCAGTCTCGT CCTAGAGTCC CACTAAATGA CAAATCTGGG 
CCGGTTCATA GGAGGTCTCC AGAAATGTCC CACCACAACT CTGATCATGT CACCGAAGAC 
TGGGAAACTG CTTCAGAAAG CAGCGACTTC AGTGAAAAGA GGCCGGGGGA TATGGATGGA 
GATGGGAGCC AGAGTGGCAG TGGGCTGTCT GAAAAACGAG AACTGTCCAA GAGAAGTTTC 
TCCAGCCAGA GACCTCTAAT GGATCGTCAG AGTCGTAAAG TGGAGCCATC AGGTTATGAT 
GAGAAGCCTT CACGGGTCAG TGGGAGTTCC TCCCGCAATG ACTTCCAGAA GAGTTCTGGT 
CCTTTAAAGA GCAGCCGCTG CTCTGACGAT TCCTATGGTG CAGAGAGCAG CCACCACCGT 
TACGCCATGG ACAGGTCTAT GCACTGCGAC AGCGGAGAGG CGGGGAAGAA ACAGGAAAGG 
GATTCAAGGA CGCCTGGTCT CAAATCCAGT GATAAAAATG AGGTGATCGG ACAGTTTGAC 
CTCAAATATG GAGATTCCAT GCTTGAGGAA GAGTCCGAGG TCGGAGACAT TGGTAACAAG 
CCTCGTCGTG TGTTGGAAAA GGATCGCCGG AAAAAGGACC AGGTGGTTCA GGTGCCGGCA 
AAGGGCAGCA GTATCCAGTC TAGGATTCCC CCTCGCTTTG CCAAAAAACA GAACGGCATG 
TGCCTGGATC AAGCCGACGT TACTGCCAAA GAAATCTGGG AGAGCAGCGG CCAAGGAATC 
TCTGTCCAGC CTGGCACGGA TACCTGGAGT AAACCTGTGA GCTCATTTAG CACGGAGTCC 
AGCTCCACAG AGGGCTTTAA AGGCAGTCAA GGAGACAGTG GCATTGACCT GAGTGCCGAG 
TCTCGGGAGT CGTCTGCTAC ATCTTCACAA CGCAGCTCTC CGTATGGAAC CATGAAACCG 
GAGGAAATGA ACGGAACAGG TGTGGCAGAG CCAAAACCAG ATTGCCCTAA AGAACAAGGG 
CAAAAACAGT CTGAGAAAAA GGATTCCGAC CCTGGCTCGG GCCTAAATAA AGAACATAAA 
CCTGGACCCA TTGGCAACGA ACGCTCGCTG AAAAACCGAA AAGGATCCGA GGGCACCGAA 
CGGTTGGAAG GCGGCGTCCC CCCAGTCAAT GGAGTAGAGA TCCACGTGGA TTCGGTTCTC 
CCTGTTCCGC CTATTGAATT TGGAGTAAGC GCAAAAGATG CAGACTATGC CCTCCCTCCT 
GGTACTGCCA CCGTTCAAGC AGCCAATACT GTTACAAAGT TGCAGGATGC TTTGGCCAGT 
AAGGCAGGGC TAACGCAGTC CATCCCCATC CTAAGGAGAG ACCACCACAT GCAGCAGGGT 
ATGAGCCTAA ACATGTCCTA CCCCACTGCG GATCTCACCC TTAAGATGGA GTCTGCCCGC 
AAAGCCTGGG AAAACTCGCC CAGTTTGCCA GAGCAGAATT CGCCAGCAGG CCCTGGCTCA 
GGAATTCAGC CGCCATCTAG TGTGGGAGCC TCCACCGGGG TCAACTACAG CTCCTTTGGC 
GGCGTGTCAA TGCCACCCAT GCCTGTTGCC TCTGTCGCCC CGTCTGCCTC CATGCCAGGA 
AACCACATCC CACCCCTGTA CCTTGATGGG CACGTGTTTA CCAACCAGCC GCGCCTGGTG 
CAGCAAACCA TACCTCAACA GCAAGGGTAT CAGCAGGCAG CAGCTGCCCA GCAGATCCCC 
ATTTCCCTGC ACACCTCCCT GCAGGCTCAA GCCCAGCTGG GTTTGCGAGG AGGCCTCCCC 
GTGTCCCAGT CGCAAGAAAT TTACAGCTCC ATGCAGCCGT TCAGGTCCCA GGTGTACATG 
CATCCAAGTC TTTCTCAGCC CAGTGCTATG GTACTGACCA GTGGCACAGG CCTCAAGCCC 
CCATACAACC CTTTCCCTGG CATGCAGACA CTGGAAATGG TTAAAACCCA GCCCACGTCC 
CCTTATCAGC CCCTGAATGG GAGCCAGCAG CTGGTGTATG AAAGCCAACT GAACCAGGCC 
TCCCAGATGA TGGACTCCCA GCTCACACAG TTGACCATGC CCATGCCCGG CTCCCAGCTC 
CAGATGCCCC GATACAGTTC AGGACAGCAA ACAATGATCT TACCACAGTC CATACAGCTT 
CCCCAGGGGC AGAACCTGCC TGTAGGGGCT CCTCGGAGAA TGCAGCCTTC TATGCTGACA 
AGCAGAGAGT CCTCCCAGAT GGAGATGAAA AGCTTCCACT TTACAGACGG CAAACAGAAC 
ATGCCGACTG CCATGCAAGC ACAGCACTCC TATCGGCCGA GTTCAGCTAG CCCCAGTGGA 
AAGTCGCCCG GACCGGGGCC ATCTGCCAAC TTGGGCTCTG TGCAGGGACA CTACCCTCAA 
CAGGTAAAGA CCAGTCCTGT CACAAAGAGC GAACCCATAT TGTACCATCT CGTTCATGTT 
CATGGCATCC TAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824435.1
CDS	319..6756
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQNVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKTSPVTKSEPILYHLVHVHGILV

Target ORF information:

RefSeq Version	XM_012968981.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968981.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAATGTCAC CAGTTGGAGA GACGGCGGTG GGAGAAGCAT CGCTTCCAGC 
TTATCTCCGA CCGCCTCTCC TACTGAGCCG GGCAGCAAAA CGGCAGTACC GGGAGAAGGG 
CCCCCCTCTA CATCGCTTGC TAATGATACT AAAGAACTCT CGCTGCGCCC GGCTCAGCCC 
ACCCGAAAAG GCCCTGCTCA GTTCATGGGA AACACCTACC ACCCACCGAC TTACCATGAC 
ATGCTACCAG CCTTTATGTG CTCTCAGCAC CCACCAGAGC CTTCTGGGAC ATTGGATCGT 
ACTTCCTTTC CCACCCTTAA TTCTCAGTCT CGCCTTGAAC CTCGAGTCCC TTTTCGCCAA 
TTTCAGATGA ATGATAACGA AGGACGGGAG AGCAGGTTTC CCATTGCACC CCCTCGTTCT 
GCACGACCCC CCCGCCCACC AGCCGAGCGC CCCCCAAGAG CCACCATCAT CAATGCAGAG 
GATCTAAAGG AGCTGGATGA CCTTGATAAT GATGCAGAGG ATGGCTGGGC TGGTCTGCAC 
GATGAGGTTG ATTATTCCGA GAAACTGAAG TTCAGTGAAG ATGAAGAAGA GGAGGACGGT 
GCTGCAAAAG ATGGCAGAAG TAAATGGAAT GGTTGGGATG GCAAAAGGCA GCGGCAGGTT 
TCCTTCAATT CTACTGATAG CGTGGAGGGA AAACATCCAG CAGATGAAGG AAAGGTGTGG 
AACGACACAT TGTCCCATTC GCGTCCTGCA AGAAGAACAC CGGAACCTGC GCCACAAGGG 
CCACGTAAAC CCAACACTTG GGCTGCTGTG GCTGATCATC AGAAACCCCC TGCCCCTGCT 
GCAGTCCTGC GACAGCCCTC CATAGAAGAG AAAGATGAAA AGCTGCCCCA GAGGAAAAAT 
TTTGTCCAGT CCGAGATCTC TGAGGCAGTA GAGCGTGCCC GCAAAAGGAG AGAGGAGGAG 
GAGAGGCGAA ACCGCGAGGA ACGGCTGGCT GCTTGTGCTG CTAAACTAAA ACAGCTTGAT 
CAGAAGAACA AGCAGGCCCT GAAACCATCG GCAGATTCTT CCCGCCAGCT GGACAATAAG 
GAGAATGAAG ACCCCCGTTC TCCTGTGTCA GACAGAAGCA ACCCCCAGGA AAACGCACAT 
ACTTTCAGAA GAGAATACTC TCAGGAGTCC CCTTCTGAAT ATCCAGAGGA GGAGCCTTCT 
GGAACCCTAA AAGCTGAAAC CAGCAGCGAA GAAGAATATG CGGAACCTAC ATCCCCAGCA 
CAAGATTTCA GCAAACATCA GAAACTGATT CCACCCCGAT TCCAGCGGCA GCAACAGCAG 
CAGGAGCAGT TGTACAAGAT GCAGCAGTGG CAGCAGCAGC AACAGCAAGC CTATGTGGCC 
TCCACTCATT CCAACCCTCC CCGGACCTTC TACTCCCCTC ACCCACAGAT GTTGGGCTTT 
GATCCACGCT GGATGATGAT GCCTTCTTAC ATGGACCCAC GGATGACGCA AGGCCGTGCG 
CCTGTGGACT TCTACCCTTC CTCCATCCAC CCTCCAGGTG TAATGAAGCA TATGATCCAG 
CAGGATTCAA TGAGCGGTTC TGGAAGTTGC CATTCAGACG AACAGAACTG CCAGTCTGAG 
AGGAGACCTC AGTCAGCAGA ACCGATGTCT GGTTGGGGCC AGGAAAGCTA CGTGCAATTA 
CACAGCAAGG CCTGCTCCCT GCCTCAGCAG AAACAGGCTG AGACTGCCGC AGTGGGGAGC 
TACAGTAGGA ATGAAAGTTC TTACTCATCC GCAAGAGAGA AATCAGATGG CTCCCCTACC 
CACCCTGAGC CCTTAGAGAG GGAAGAGGAG TATTTATCTG TTTCTTATGA AAAGAAATCC 
ACTGGGAGCT TTAGCAGTTG CATTTCCCCT CAAAGGAGAG GACAGGACGC CTTGTACCAA 
CATCTAGAGA GCGGCATAGA GTCTGGTGTG AACAGGGGGA GACAGATGGC AGCCAGGGAT 
TCGTCCTCAA GAGGCTCTGA GTTTATAAAG ACTGATAAAA AGCCACAGTT TAATGGCTGG 
GGTTATGCAC CCCATCAGAA ATCTTCCGAA ACAGCCAGCA GAGCAGAAGA GGATCTTTCA 
AAGGAGGACC TATCTGATGA ACTGGAGCAC TGGAAGAAGG AGGATGGTGG GAGCAGCCAG 
GATGGTACAG GAGAAACCTG CTGGAGAAGT GAATCTGCAA ATTCCAACAG TCAGCAGCAA 
TCTGAGCATC TAGGGAGAAG CCGTAGATCT GGACCTATCA AAAAGCCCGT CTTGAAAGCC 
CTCAAGATAG AGGACAAAGA GCTGGAGAAA AACAAGGCAG AAATTAAAGA GCCAGTAAAA 
CCTTTTAAGG AAAAAATTCT TCCAAAAACA GAGTGCGTTG TTAAGACTGA ACCTGTTGTT 
TCAAGCCCTC CTCCCAGCAT GATTGAAGTT AAAACTCCCC CTCCGCCTAG TCCTGTCCAG 
GAGACTGATA AATTCCCCCA AGAAAAAGCT GAAAGGAGTT GGGAGACCAA GCCTTTACGG 
GAATCTAGTA GCACTTCTTT GCAAAAGAGA AGTGACTGGA TATTCATTGA CGAAGAGCAA 
GCGTTTGGCG GTAGGACTCA AGGCCGTGGC AGAGGTAGAG GGTTTAAAGA CTTTGGTTTC 
CGCAACCGAG GGGTCTCTGG CACTTACAGC GGGCAGAAGG TCAGCCGAGG TCGGGGTGTT 
CGAGAGTACA ATCCCCCTGA AGATCTTAGG GGCAAGGCCT CCCGGAGGAG GATTGCAAGC 
GAAACACACA GTGAGGGGTC AGAATATGAA GAGCTTCCGA AACGCAGGAG GCAGAGAGGG 
ACGGAGAATG GCAATGATCC TGCTGTACAG GAGAGGGAAG CTGAAGATCT GCGAAAGGGG 
GACTTTCAGG ATTCATGGAG ATCTAATAGG AATTATTCAG ACGATCAGAA TAACACTGAC 
TCAAAATCAA GAGCTCCAAG AGCTTTTGGA AGGTCCCTTC CGCCAAGGTT GAGCAACAGC 
TATGGTAGGA GACCATTATC CACCAAGGAG TCCTCTCACT GGCAAGCCAA GTCTGGGGGA 
TCGTCTTGGC AAGAGTATGG GGCGCCAGCT GAAGCCTATG GGAGTCGCCA TCATGCAGAC 
AGAGACTATG CCCATGACTA TAGATACGCA GACTCATTTC CTAGTAGGGG TTTTGATGAA 
AGTCACGGAG ATGAGAGGAG GTCTTTTTTC CAAGAGGAAT ATTCAGACCG GGATAGTTTG 
GAAAAGAGAT CTTTTGGCAG GCGAAGGCCC CCACGCCAAG ACAAACCTCC GAGGTTTAGG 
CGTCTTAGAC AGGAAAGAGA CCCTTTGGGA CAGTGGAGTG GTGAAGAGAC AGTCGGCACT 
GGAAACAGCA GTGAACACTG GCAGTCTCGT CCTAGAGTCC CACTAAATGA CAAATCTGGG 
CCGGTTCATA GGAGGTCTCC AGAAATGTCC CACCACAACT CTGATCATGT CACCGAAGAC 
TGGGAAACTG CTTCAGAAAG CAGCGACTTC AGTGAAAAGA GGCCGGGGGA TATGGATGGA 
GATGGGAGCC AGAGTGGCAG TGGGCTGTCT GAAAAACGAG AACTGTCCAA GAGAAGTTTC 
TCCAGCCAGA GACCTCTAAT GGATCGTCAG AGTCGTAAAG TGGAGCCATC AGGTTATGAT 
GAGAAGCCTT CACGGGTCAG TGGGAGTTCC TCCCGCAATG ACTTCCAGAA GAGTTCTGGT 
CCTTTAAAGA GCAGCCGCTG CTCTGACGAT TCCTATGGTG CAGAGAGCAG CCACCACCGT 
TACGCCATGG ACAGGTCTAT GCACTGCGAC AGCGGAGAGG CGGGGAAGAA ACAGGAAAGG 
GATTCAAGGA CGCCTGGTCT CAAATCCAGT GATAAAAATG AGGTGATCGG ACAGTTTGAC 
CTCAAATATG GAGATTCCAT GCTTGAGGAA GAGTCCGAGG TCGGAGACAT TGGTAACAAG 
CCTCGTCGTG TGTTGGAAAA GGATCGCCGG AAAAAGGACC AGGTGGTTCA GGTGCCGGCA 
AAGGGCAGCA GTATCCAGTC TAGGATTCCC CCTCGCTTTG CCAAAAAACA GAACGGCATG 
TGCCTGGATC AAGCCGACGT TACTGCCAAA GAAATCTGGG AGAGCAGCGG CCAAGGAATC 
TCTGTCCAGC CTGGCACGGA TACCTGGAGT AAACCTGTGA GCTCATTTAG CACGGAGTCC 
AGCTCCACAG AGGGCTTTAA AGGCAGTCAA GGAGACAGTG GCATTGACCT GAGTGCCGAG 
TCTCGGGAGT CGTCTGCTAC ATCTTCACAA CGCAGCTCTC CGTATGGAAC CATGAAACCG 
GAGGAAATGA ACGGAACAGG TGTGGCAGAG CCAAAACCAG ATTGCCCTAA AGAACAAGGG 
CAAAAACAGT CTGAGAAAAA GGATTCCGAC CCTGGCTCGG GCCTAAATAA AGAACATAAA 
CCTGGACCCA TTGGCAACGA ACGCTCGCTG AAAAACCGAA AAGGATCCGA GGGCACCGAA 
CGGTTGGAAG GCGGCGTCCC CCCAGTCAAT GGAGTAGAGA TCCACGTGGA TTCGGTTCTC 
CCTGTTCCGC CTATTGAATT TGGAGTAAGC GCAAAAGATG CAGACTATGC CCTCCCTCCT 
GGTACTGCCA CCGTTCAAGC AGCCAATACT GTTACAAAGT TGCAGGATGC TTTGGCCAGT 
AAGGCAGGGC TAACGCAGTC CATCCCCATC CTAAGGAGAG ACCACCACAT GCAGCAGGGT 
ATGAGCCTAA ACATGTCCTA CCCCACTGCG GATCTCACCC TTAAGATGGA GTCTGCCCGC 
AAAGCCTGGG AAAACTCGCC CAGTTTGCCA GAGCAGAATT CGCCAGCAGG CCCTGGCTCA 
GGAATTCAGC CGCCATCTAG TGTGGGAGCC TCCACCGGGG TCAACTACAG CTCCTTTGGC 
GGCGTGTCAA TGCCACCCAT GCCTGTTGCC TCTGTCGCCC CGTCTGCCTC CATGCCAGGA 
AACCACATCC CACCCCTGTA CCTTGATGGG CACGTGTTTA CCAACCAGCC GCGCCTGGTG 
CAGCAAACCA TACCTCAACA GCAAGGGTAT CAGCAGGCAG CAGCTGCCCA GCAGATCCCC 
ATTTCCCTGC ACACCTCCCT GCAGGCTCAA GCCCAGCTGG GTTTGCGAGG AGGCCTCCCC 
GTGTCCCAGT CGCAAGAAAT TTACAGCTCC ATGCAGCCGT TCAGGTCCCA GGTGTACATG 
CATCCAAGTC TTTCTCAGCC CAGTGCTATG GTACTGACCA GTGGCACAGG CCTCAAGCCC 
CCATACAACC CTTTCCCTGG CATGCAGACA CTGGAAATGG TTAAAACCCA GCCCACGTCC 
CCTTATCAGC CCCTGAATGG GAGCCAGCAG CTGGTGTATG AAAGCCAACT GAACCAGGCC 
TCCCAGATGA TGGACTCCCA GCTCACACAG TTGACCATGC CCATGCCCGG CTCCCAGCTC 
CAGATGCCCC GATACAGTTC AGGACAGCAA ACAATGATCT TACCACAGTC CATACAGCTT 
CCCCAGGGGC AGAACCTGCC TGTAGGGGCT CCTCGGAGAA TGCAGCCTTC TATGCTGACA 
AGCAGAGAGT CCTCCCAGAT GGAGATGAAA AGCTTCCACT TTACAGACGG CAAACAGAAC 
ATGCCGACTG CCATGCAAGC ACAGCACTCC TATCGGCCGA GTTCAGCTAG CCCCAGTGGA 
AAGTCGCCCG GACCGGGGCC ATCTGCCAAC TTGGGCTCTG TGCAGGGACA CTACCCTCAA 
CAGGTAAAGA CCAGTCCTGT CACAAAGAGC GAACCCATAT TGTACCATCT CGTTCATGTT 
CATGGCATCC TAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968982.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824436.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

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CloneID	OXa35274
Clone ID Related Accession (Same CDS sequence)	XM_012968982.2 , XM_012968982.3
Accession Version	XM_012968982.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TGGCAAGGTG ACCTCTTCCA GATAAAGGAG ACTCCTCCCA TCTGTTGGCG GAAGGTTCTG 
CATGCCCGGC CCGTGTCCCC TGCACAAATG GCTTTGGAAT TACCATTCTC TGCTCTCGCC 
CCCAACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824436.1
CDS	319..6750
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRWQGDLFQIKETPPICWRKVLHARPVSPAQMALELPFSALAPNR

Target ORF information:

RefSeq Version	XM_012968982.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968982.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TGGCAAGGTG ACCTCTTCCA GATAAAGGAG ACTCCTCCCA TCTGTTGGCG GAAGGTTCTG 
CATGCCCGGC CCGTGTCCCC TGCACAAATG GCTTTGGAAT TACCATTCTC TGCTCTCGCC 
CCCAACAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968983.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824437.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

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CloneID	OXa35275
Clone ID Related Accession (Same CDS sequence)	XM_012968983.2
Accession Version	XM_012968983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GCAGGGCTAA CGCAGTCCAT CCCCATCCTA AGGAGAGACC ACCACATGCA GCAGGGTATG 
AGCCTAAACA TGTCCTACCC CACTGCGGAT CTCACCCTTA AGATGGAGTC TGCCCGCAAA 
GCCTGGGAAA ACTCGCCCAG TTTGCCAGAG CAGAATTCGC CAGCAGGCCC TGGCTCAGGA 
ATTCAGCCGC CATCTAGTGT GGGAGCCTCC ACCGGGGTCA ACTACAGCTC CTTTGGCGGC 
GTGTCAATGC CACCCATGCC TGTTGCCTCT GTCGCCCCGT CTGCCTCCAT GCCAGGAAAC 
CACATCCCAC CCCTGTACCT TGATGGGCAC GTGTTTACCA ACCAGCCGCG CCTGGTGCAG 
CAAACCATAC CTCAACAGCA AGGGTATCAG CAGGCAGCAG CTGCCCAGCA GATCCCCATT 
TCCCTGCACA CCTCCCTGCA GGCTCAAGCC CAGCTGGGTT TGCGAGGAGG CCTCCCCGTG 
TCCCAGTCGC AAGAAATTTA CAGCTCCATG CAGCCGTTCA GGTCCCAGGT GTACATGCAT 
CCAAGTCTTT CTCAGCCCAG TGCTATGGTA CTGACCAGTG GCACAGGCCT CAAGCCCCCA 
TACAACCCTT TCCCTGGCAT GCAGACACTG GAAATGGTTA AAACCCAGCC CACGTCCCCT 
TATCAGCCCC TGAATGGGAG CCAGCAGCTG GTGTATGAAA GCCAACTGAA CCAGGCCTCC 
CAGATGATGG ACTCCCAGCT CACACAGTTG ACCATGCCCA TGCCCGGCTC CCAGCTCCAG 
ATGCCCCGAT ACAGTTCAGG ACAGCAAACA ATGATCTTAC CACAGTCCAT ACAGCTTCCC 
CAGGGGCAGA ACCTGCCTGT AGGGGCTCCT CGGAGAATGC AGCCTTCTAT GCTGACAAGC 
AGAGAGTCCT CCCAGATGGA GATGAAAAGC TTCCACTTTA CAGACGGCAA ACAGAACATG 
CCGACTGCCA TGCAAGCACA GCACTCCTAT CGGCCGAGTT CAGCTAGCCC CAGTGGAAAG 
TCGCCCGGAC CGGGGCCATC TGCCAACTTG GGCTCTGTGC AGGGACACTA CCCTCAACAG 
GTAAAGACCA GTCCTGTCAC AAAGAGCGAA CCCATATTGT ACCATCTCGT TCATGTTCAT 
GGCATCCTAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824437.1
CDS	319..6693
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYRPSSASPSGKSPGPGPSANLGSVQGHYPQQVKTSPVTKSEPILYHLVHVHGILV

Target ORF information:

RefSeq Version	XM_012968983.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968983.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GCAGGGCTAA CGCAGTCCAT CCCCATCCTA AGGAGAGACC ACCACATGCA GCAGGGTATG 
AGCCTAAACA TGTCCTACCC CACTGCGGAT CTCACCCTTA AGATGGAGTC TGCCCGCAAA 
GCCTGGGAAA ACTCGCCCAG TTTGCCAGAG CAGAATTCGC CAGCAGGCCC TGGCTCAGGA 
ATTCAGCCGC CATCTAGTGT GGGAGCCTCC ACCGGGGTCA ACTACAGCTC CTTTGGCGGC 
GTGTCAATGC CACCCATGCC TGTTGCCTCT GTCGCCCCGT CTGCCTCCAT GCCAGGAAAC 
CACATCCCAC CCCTGTACCT TGATGGGCAC GTGTTTACCA ACCAGCCGCG CCTGGTGCAG 
CAAACCATAC CTCAACAGCA AGGGTATCAG CAGGCAGCAG CTGCCCAGCA GATCCCCATT 
TCCCTGCACA CCTCCCTGCA GGCTCAAGCC CAGCTGGGTT TGCGAGGAGG CCTCCCCGTG 
TCCCAGTCGC AAGAAATTTA CAGCTCCATG CAGCCGTTCA GGTCCCAGGT GTACATGCAT 
CCAAGTCTTT CTCAGCCCAG TGCTATGGTA CTGACCAGTG GCACAGGCCT CAAGCCCCCA 
TACAACCCTT TCCCTGGCAT GCAGACACTG GAAATGGTTA AAACCCAGCC CACGTCCCCT 
TATCAGCCCC TGAATGGGAG CCAGCAGCTG GTGTATGAAA GCCAACTGAA CCAGGCCTCC 
CAGATGATGG ACTCCCAGCT CACACAGTTG ACCATGCCCA TGCCCGGCTC CCAGCTCCAG 
ATGCCCCGAT ACAGTTCAGG ACAGCAAACA ATGATCTTAC CACAGTCCAT ACAGCTTCCC 
CAGGGGCAGA ACCTGCCTGT AGGGGCTCCT CGGAGAATGC AGCCTTCTAT GCTGACAAGC 
AGAGAGTCCT CCCAGATGGA GATGAAAAGC TTCCACTTTA CAGACGGCAA ACAGAACATG 
CCGACTGCCA TGCAAGCACA GCACTCCTAT CGGCCGAGTT CAGCTAGCCC CAGTGGAAAG 
TCGCCCGGAC CGGGGCCATC TGCCAACTTG GGCTCTGTGC AGGGACACTA CCCTCAACAG 
GTAAAGACCA GTCCTGTCAC AAAGAGCGAA CCCATATTGT ACCATCTCGT TCATGTTCAT 
GGCATCCTAG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_018096599.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952088.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.

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CloneID	OXa35276
Clone ID Related Accession (Same CDS sequence)	XM_018096599.1 , XM_012968984.2 , XM_012968984.3
Accession Version	XM_018096599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952088.1
CDS	319..6621
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYR

Target ORF information:

RefSeq Version	XM_018096599.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096599.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_012968984.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824438.1 Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.

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CloneID	OXa35276
Clone ID Related Accession (Same CDS sequence)	XM_018096599.1 , XM_012968984.2 , XM_012968984.3
Accession Version	XM_012968984.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein PRRC2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824438.1
CDS	319..6621
Translation	MSDRLGQITKGKDGKSKYSSLNLFDKYKGKSIEAVRTTVIPRHGLQSLGKVAAARRMPPPANLPSLKSENKGNDPNIFIVPKDGTGWANKQEQQDQKSSSVTAPPQQESLPPQGLQKSVSNLQKPTPSINQENINLVPGGPKSWAQLNGKPAGQEGGSRGSNRLLSFSPEEFPTLKAAGEQDKAGKEKSVLDPSYGPGPSLRPQSKPLALLPDVTSWRDGGGRSIASSLSPTASPTEPGSKTAVPGEGPPSTSLANDTKELSLRPAQPTRKGPAQFMGNTYHPPTYHDMLPAFMCSQHPPEPSGTLDRTSFPTLNSQSRLEPRVPFRQFQMNDNEGRESRFPIAPPRSARPPRPPAERPPRATIINAEDLKELDDLDNDAEDGWAGLHDEVDYSEKLKFSEDEEEEDGAAKDGRSKWNGWDGKRQRQVSFNSTDSVEGKHPADEGKVWNDTLSHSRPARRTPEPAPQGPRKPNTWAAVADHQKPPAPAAVLRQPSIEEKDEKLPQRKNFVQSEISEAVERARKRREEEERRNREERLAACAAKLKQLDQKNKQALKPSADSSRQLDNKENEDPRSPVSDRSNPQENAHTFRREYSQESPSEYPEEEPSGTLKAETSSEEEYAEPTSPAQDFSKHQKLIPPRFQRQQQQQEQLYKMQQWQQQQQQAYVASTHSNPPRTFYSPHPQMLGFDPRWMMMPSYMDPRMTQGRAPVDFYPSSIHPPGVMKHMIQQDSMSGSGSCHSDEQNCQSERRPQSAEPMSGWGQESYVQLHSKACSLPQQKQAETAAVGSYSRNESSYSSAREKSDGSPTHPEPLEREEEYLSVSYEKKSTGSFSSCISPQRRGQDALYQHLESGIESGVNRGRQMAARDSSSRGSEFIKTDKKPQFNGWGYAPHQKSSETASRAEEDLSKEDLSDELEHWKKEDGGSSQDGTGETCWRSESANSNSQQQSEHLGRSRRSGPIKKPVLKALKIEDKELEKNKAEIKEPVKPFKEKILPKTECVVKTEPVVSSPPPSMIEVKTPPPPSPVQETDKFPQEKAERSWETKPLRESSSTSLQKRSDWIFIDEEQAFGGRTQGRGRGRGFKDFGFRNRGVSGTYSGQKVSRGRGVREYNPPEDLRGKASRRRIASETHSEGSEYEELPKRRRQRGTENGNDPAVQEREAEDLRKGDFQDSWRSNRNYSDDQNNTDSKSRAPRAFGRSLPPRLSNSYGRRPLSTKESSHWQAKSGGSSWQEYGAPAEAYGSRHHADRDYAHDYRYADSFPSRGFDESHGDERRSFFQEEYSDRDSLEKRSFGRRRPPRQDKPPRFRRLRQERDPLGQWSGEETVGTGNSSEHWQSRPRVPLNDKSGPVHRRSPEMSHHNSDHVTEDWETASESSDFSEKRPGDMDGDGSQSGSGLSEKRELSKRSFSSQRPLMDRQSRKVEPSGYDEKPSRVSGSSSRNDFQKSSGPLKSSRCSDDSYGAESSHHRYAMDRSMHCDSGEAGKKQERDSRTPGLKSSDKNEVIGQFDLKYGDSMLEEESEVGDIGNKPRRVLEKDRRKKDQVVQVPAKGSSIQSRIPPRFAKKQNGMCLDQADVTAKEIWESSGQGISVQPGTDTWSKPVSSFSTESSSTEGFKGSQGDSGIDLSAESRESSATSSQRSSPYGTMKPEEMNGTGVAEPKPDCPKEQGQKQSEKKDSDPGSGLNKEHKPGPIGNERSLKNRKGSEGTERLEGGVPPVNGVEIHVDSVLPVPPIEFGVSAKDADYALPPGTATVQAANTVTKLQDALASKAGLTQSIPILRRDHHMQQGMSLNMSYPTADLTLKMESARKAWENSPSLPEQNSPAGPGSGIQPPSSVGASTGVNYSSFGGVSMPPMPVASVAPSASMPGNHIPPLYLDGHVFTNQPRLVQQTIPQQQGYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEIYSSMQPFRSQVYMHPSLSQPSAMVLTSGTGLKPPYNPFPGMQTLEMVKTQPTSPYQPLNGSQQLVYESQLNQASQMMDSQLTQLTMPMPGSQLQMPRYSSGQQTMILPQSIQLPQGQNLPVGAPRRMQPSMLTSRESSQMEMKSFHFTDGKQNMPTAMQAQHSYR

Target ORF information:

RefSeq Version	XM_012968984.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis proline-rich coiled-coil 2B (prrc2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012968984.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA GATAACCAAG GGAAAGGATG GGAAAAGCAA GTATTCGTCT 
CTCAACCTGT TTGACAAGTA TAAAGGGAAG TCAATAGAAG CAGTCCGAAC CACAGTTATA 
CCTAGACATG GCTTACAGAG TCTTGGGAAA GTTGCCGCAG CCCGGCGCAT GCCCCCTCCT 
GCAAACTTGC CAAGCTTGAA ATCTGAAAAC AAAGGAAACG ACCCCAACAT TTTTATAGTG 
CCCAAGGACG GAACGGGATG GGCAAACAAA CAAGAACAGC AAGACCAAAA GAGTTCCAGT 
GTGACAGCTC CTCCGCAGCA GGAGTCGCTG CCGCCGCAGG GTTTGCAGAA ATCTGTCTCC 
AATTTACAGA AGCCGACCCC ATCGATCAAT CAGGAGAACA TAAATTTAGT GCCAGGTGGA 
CCAAAATCAT GGGCACAGCT GAATGGAAAG CCAGCAGGAC AAGAAGGTGG TTCAAGGGGC 
TCAAACCGAC TGTTGTCCTT CTCTCCCGAG GAATTTCCAA CGCTGAAAGC AGCTGGCGAG 
CAAGACAAGG CTGGCAAAGA AAAGAGCGTC TTAGATCCGT CGTATGGGCC CGGACCAAGC 
CTCCGCCCCC AGAGTAAGCC CCTCGCCCTG CTTCCCGATG TCACCAGTTG GAGAGACGGC 
GGTGGGAGAA GCATCGCTTC CAGCTTATCT CCGACCGCCT CTCCTACTGA GCCGGGCAGC 
AAAACGGCAG TACCGGGAGA AGGGCCCCCC TCTACATCGC TTGCTAATGA TACTAAAGAA 
CTCTCGCTGC GCCCGGCTCA GCCCACCCGA AAAGGCCCTG CTCAGTTCAT GGGAAACACC 
TACCACCCAC CGACTTACCA TGACATGCTA CCAGCCTTTA TGTGCTCTCA GCACCCACCA 
GAGCCTTCTG GGACATTGGA TCGTACTTCC TTTCCCACCC TTAATTCTCA GTCTCGCCTT 
GAACCTCGAG TCCCTTTTCG CCAATTTCAG ATGAATGATA ACGAAGGACG GGAGAGCAGG 
TTTCCCATTG CACCCCCTCG TTCTGCACGA CCCCCCCGCC CACCAGCCGA GCGCCCCCCA 
AGAGCCACCA TCATCAATGC AGAGGATCTA AAGGAGCTGG ATGACCTTGA TAATGATGCA 
GAGGATGGCT GGGCTGGTCT GCACGATGAG GTTGATTATT CCGAGAAACT GAAGTTCAGT 
GAAGATGAAG AAGAGGAGGA CGGTGCTGCA AAAGATGGCA GAAGTAAATG GAATGGTTGG 
GATGGCAAAA GGCAGCGGCA GGTTTCCTTC AATTCTACTG ATAGCGTGGA GGGAAAACAT 
CCAGCAGATG AAGGAAAGGT GTGGAACGAC ACATTGTCCC ATTCGCGTCC TGCAAGAAGA 
ACACCGGAAC CTGCGCCACA AGGGCCACGT AAACCCAACA CTTGGGCTGC TGTGGCTGAT 
CATCAGAAAC CCCCTGCCCC TGCTGCAGTC CTGCGACAGC CCTCCATAGA AGAGAAAGAT 
GAAAAGCTGC CCCAGAGGAA AAATTTTGTC CAGTCCGAGA TCTCTGAGGC AGTAGAGCGT 
GCCCGCAAAA GGAGAGAGGA GGAGGAGAGG CGAAACCGCG AGGAACGGCT GGCTGCTTGT 
GCTGCTAAAC TAAAACAGCT TGATCAGAAG AACAAGCAGG CCCTGAAACC ATCGGCAGAT 
TCTTCCCGCC AGCTGGACAA TAAGGAGAAT GAAGACCCCC GTTCTCCTGT GTCAGACAGA 
AGCAACCCCC AGGAAAACGC ACATACTTTC AGAAGAGAAT ACTCTCAGGA GTCCCCTTCT 
GAATATCCAG AGGAGGAGCC TTCTGGAACC CTAAAAGCTG AAACCAGCAG CGAAGAAGAA 
TATGCGGAAC CTACATCCCC AGCACAAGAT TTCAGCAAAC ATCAGAAACT GATTCCACCC 
CGATTCCAGC GGCAGCAACA GCAGCAGGAG CAGTTGTACA AGATGCAGCA GTGGCAGCAG 
CAGCAACAGC AAGCCTATGT GGCCTCCACT CATTCCAACC CTCCCCGGAC CTTCTACTCC 
CCTCACCCAC AGATGTTGGG CTTTGATCCA CGCTGGATGA TGATGCCTTC TTACATGGAC 
CCACGGATGA CGCAAGGCCG TGCGCCTGTG GACTTCTACC CTTCCTCCAT CCACCCTCCA 
GGTGTAATGA AGCATATGAT CCAGCAGGAT TCAATGAGCG GTTCTGGAAG TTGCCATTCA 
GACGAACAGA ACTGCCAGTC TGAGAGGAGA CCTCAGTCAG CAGAACCGAT GTCTGGTTGG 
GGCCAGGAAA GCTACGTGCA ATTACACAGC AAGGCCTGCT CCCTGCCTCA GCAGAAACAG 
GCTGAGACTG CCGCAGTGGG GAGCTACAGT AGGAATGAAA GTTCTTACTC ATCCGCAAGA 
GAGAAATCAG ATGGCTCCCC TACCCACCCT GAGCCCTTAG AGAGGGAAGA GGAGTATTTA 
TCTGTTTCTT ATGAAAAGAA ATCCACTGGG AGCTTTAGCA GTTGCATTTC CCCTCAAAGG 
AGAGGACAGG ACGCCTTGTA CCAACATCTA GAGAGCGGCA TAGAGTCTGG TGTGAACAGG 
GGGAGACAGA TGGCAGCCAG GGATTCGTCC TCAAGAGGCT CTGAGTTTAT AAAGACTGAT 
AAAAAGCCAC AGTTTAATGG CTGGGGTTAT GCACCCCATC AGAAATCTTC CGAAACAGCC 
AGCAGAGCAG AAGAGGATCT TTCAAAGGAG GACCTATCTG ATGAACTGGA GCACTGGAAG 
AAGGAGGATG GTGGGAGCAG CCAGGATGGT ACAGGAGAAA CCTGCTGGAG AAGTGAATCT 
GCAAATTCCA ACAGTCAGCA GCAATCTGAG CATCTAGGGA GAAGCCGTAG ATCTGGACCT 
ATCAAAAAGC CCGTCTTGAA AGCCCTCAAG ATAGAGGACA AAGAGCTGGA GAAAAACAAG 
GCAGAAATTA AAGAGCCAGT AAAACCTTTT AAGGAAAAAA TTCTTCCAAA AACAGAGTGC 
GTTGTTAAGA CTGAACCTGT TGTTTCAAGC CCTCCTCCCA GCATGATTGA AGTTAAAACT 
CCCCCTCCGC CTAGTCCTGT CCAGGAGACT GATAAATTCC CCCAAGAAAA AGCTGAAAGG 
AGTTGGGAGA CCAAGCCTTT ACGGGAATCT AGTAGCACTT CTTTGCAAAA GAGAAGTGAC 
TGGATATTCA TTGACGAAGA GCAAGCGTTT GGCGGTAGGA CTCAAGGCCG TGGCAGAGGT 
AGAGGGTTTA AAGACTTTGG TTTCCGCAAC CGAGGGGTCT CTGGCACTTA CAGCGGGCAG 
AAGGTCAGCC GAGGTCGGGG TGTTCGAGAG TACAATCCCC CTGAAGATCT TAGGGGCAAG 
GCCTCCCGGA GGAGGATTGC AAGCGAAACA CACAGTGAGG GGTCAGAATA TGAAGAGCTT 
CCGAAACGCA GGAGGCAGAG AGGGACGGAG AATGGCAATG ATCCTGCTGT ACAGGAGAGG 
GAAGCTGAAG ATCTGCGAAA GGGGGACTTT CAGGATTCAT GGAGATCTAA TAGGAATTAT 
TCAGACGATC AGAATAACAC TGACTCAAAA TCAAGAGCTC CAAGAGCTTT TGGAAGGTCC 
CTTCCGCCAA GGTTGAGCAA CAGCTATGGT AGGAGACCAT TATCCACCAA GGAGTCCTCT 
CACTGGCAAG CCAAGTCTGG GGGATCGTCT TGGCAAGAGT ATGGGGCGCC AGCTGAAGCC 
TATGGGAGTC GCCATCATGC AGACAGAGAC TATGCCCATG ACTATAGATA CGCAGACTCA 
TTTCCTAGTA GGGGTTTTGA TGAAAGTCAC GGAGATGAGA GGAGGTCTTT TTTCCAAGAG 
GAATATTCAG ACCGGGATAG TTTGGAAAAG AGATCTTTTG GCAGGCGAAG GCCCCCACGC 
CAAGACAAAC CTCCGAGGTT TAGGCGTCTT AGACAGGAAA GAGACCCTTT GGGACAGTGG 
AGTGGTGAAG AGACAGTCGG CACTGGAAAC AGCAGTGAAC ACTGGCAGTC TCGTCCTAGA 
GTCCCACTAA ATGACAAATC TGGGCCGGTT CATAGGAGGT CTCCAGAAAT GTCCCACCAC 
AACTCTGATC ATGTCACCGA AGACTGGGAA ACTGCTTCAG AAAGCAGCGA CTTCAGTGAA 
AAGAGGCCGG GGGATATGGA TGGAGATGGG AGCCAGAGTG GCAGTGGGCT GTCTGAAAAA 
CGAGAACTGT CCAAGAGAAG TTTCTCCAGC CAGAGACCTC TAATGGATCG TCAGAGTCGT 
AAAGTGGAGC CATCAGGTTA TGATGAGAAG CCTTCACGGG TCAGTGGGAG TTCCTCCCGC 
AATGACTTCC AGAAGAGTTC TGGTCCTTTA AAGAGCAGCC GCTGCTCTGA CGATTCCTAT 
GGTGCAGAGA GCAGCCACCA CCGTTACGCC ATGGACAGGT CTATGCACTG CGACAGCGGA 
GAGGCGGGGA AGAAACAGGA AAGGGATTCA AGGACGCCTG GTCTCAAATC CAGTGATAAA 
AATGAGGTGA TCGGACAGTT TGACCTCAAA TATGGAGATT CCATGCTTGA GGAAGAGTCC 
GAGGTCGGAG ACATTGGTAA CAAGCCTCGT CGTGTGTTGG AAAAGGATCG CCGGAAAAAG 
GACCAGGTGG TTCAGGTGCC GGCAAAGGGC AGCAGTATCC AGTCTAGGAT TCCCCCTCGC 
TTTGCCAAAA AACAGAACGG CATGTGCCTG GATCAAGCCG ACGTTACTGC CAAAGAAATC 
TGGGAGAGCA GCGGCCAAGG AATCTCTGTC CAGCCTGGCA CGGATACCTG GAGTAAACCT 
GTGAGCTCAT TTAGCACGGA GTCCAGCTCC ACAGAGGGCT TTAAAGGCAG TCAAGGAGAC 
AGTGGCATTG ACCTGAGTGC CGAGTCTCGG GAGTCGTCTG CTACATCTTC ACAACGCAGC 
TCTCCGTATG GAACCATGAA ACCGGAGGAA ATGAACGGAA CAGGTGTGGC AGAGCCAAAA 
CCAGATTGCC CTAAAGAACA AGGGCAAAAA CAGTCTGAGA AAAAGGATTC CGACCCTGGC 
TCGGGCCTAA ATAAAGAACA TAAACCTGGA CCCATTGGCA ACGAACGCTC GCTGAAAAAC 
CGAAAAGGAT CCGAGGGCAC CGAACGGTTG GAAGGCGGCG TCCCCCCAGT CAATGGAGTA 
GAGATCCACG TGGATTCGGT TCTCCCTGTT CCGCCTATTG AATTTGGAGT AAGCGCAAAA 
GATGCAGACT ATGCCCTCCC TCCTGGTACT GCCACCGTTC AAGCAGCCAA TACTGTTACA 
AAGTTGCAGG ATGCTTTGGC CAGTAAGGCA GGGCTAACGC AGTCCATCCC CATCCTAAGG 
AGAGACCACC ACATGCAGCA GGGTATGAGC CTAAACATGT CCTACCCCAC TGCGGATCTC 
ACCCTTAAGA TGGAGTCTGC CCGCAAAGCC TGGGAAAACT CGCCCAGTTT GCCAGAGCAG 
AATTCGCCAG CAGGCCCTGG CTCAGGAATT CAGCCGCCAT CTAGTGTGGG AGCCTCCACC 
GGGGTCAACT ACAGCTCCTT TGGCGGCGTG TCAATGCCAC CCATGCCTGT TGCCTCTGTC 
GCCCCGTCTG CCTCCATGCC AGGAAACCAC ATCCCACCCC TGTACCTTGA TGGGCACGTG 
TTTACCAACC AGCCGCGCCT GGTGCAGCAA ACCATACCTC AACAGCAAGG GTATCAGCAG 
GCAGCAGCTG CCCAGCAGAT CCCCATTTCC CTGCACACCT CCCTGCAGGC TCAAGCCCAG 
CTGGGTTTGC GAGGAGGCCT CCCCGTGTCC CAGTCGCAAG AAATTTACAG CTCCATGCAG 
CCGTTCAGGT CCCAGGTGTA CATGCATCCA AGTCTTTCTC AGCCCAGTGC TATGGTACTG 
ACCAGTGGCA CAGGCCTCAA GCCCCCATAC AACCCTTTCC CTGGCATGCA GACACTGGAA 
ATGGTTAAAA CCCAGCCCAC GTCCCCTTAT CAGCCCCTGA ATGGGAGCCA GCAGCTGGTG 
TATGAAAGCC AACTGAACCA GGCCTCCCAG ATGATGGACT CCCAGCTCAC ACAGTTGACC 
ATGCCCATGC CCGGCTCCCA GCTCCAGATG CCCCGATACA GTTCAGGACA GCAAACAATG 
ATCTTACCAC AGTCCATACA GCTTCCCCAG GGGCAGAACC TGCCTGTAGG GGCTCCTCGG 
AGAATGCAGC CTTCTATGCT GACAAGCAGA GAGTCCTCCC AGATGGAGAT GAAAAGCTTC 
CACTTTACAG ACGGCAAACA GAACATGCCG ACTGCCATGC AAGCACAGCA CTCCTATCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	prrc2b
Entrez Gene ID	100492119
Full Name	proline-rich coiled-coil 2B
Synonyms	LQFBS-1,bat2l
General protein information	Preferred Names proline-rich coiled-coil 2B Names protein PRRC2B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	LOC561451	XP_005172099.1
Xenopus tropicalis (tropical clawed frog)	prrc2b	XP_002936236.2
Homo sapiens (human)	PRRC2B	NP_037450.2
Mus musculus (house mouse)	Prrc2b	NP_001153106.1
	LOC100425699	XP_002800001.1
Canis lupus familiaris (dog)	PRRC2B	XP_548409.3
Rattus norvegicus (Norway rat)	Prrc2b	XP_006234067.1
Pan troglodytes (chimpanzee)	PRRC2B	XP_520327.2
Bos taurus (cattle)	PRRC2B	XP_005213498.1
Gallus gallus (chicken)	PRRC2B	XP_415459.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

prrc2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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