PRRC2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRRC2B
Entrez Gene ID	561451
Full Name	proline-rich coiled-coil 2B
General protein information	Preferred Names proline-rich coiled-coil 2B Names protein PRRC2B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172044.3	XP_005172101.1	protein PRRC2B isoform X3
XM_005172045.3	XP_005172102.1	protein PRRC2B isoform X4
XM_005172046.4	XP_005172103.1	protein PRRC2B isoform X2
XM_005172043.3	XP_005172100.1	protein PRRC2B isoform X2
XM_005172042.4	XP_005172099.1	protein PRRC2B isoform X1
XM_005172042.3	XP_005172099.1	protein PRRC2B isoform X1
XM_005172046.3	XP_005172103.1	protein PRRC2B isoform X5

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC561451	XP_005172099.1
Xenopus tropicalis (tropical clawed frog)	prrc2b	XP_002936236.2
Homo sapiens (human)	PRRC2B	NP_037450.2
Mus musculus (house mouse)	Prrc2b	NP_001153106.1
	LOC100425699	XP_002800001.1
Canis lupus familiaris (dog)	PRRC2B	XP_548409.3
Rattus norvegicus (Norway rat)	Prrc2b	XP_006234067.1
Pan troglodytes (chimpanzee)	PRRC2B	XP_520327.2
Bos taurus (cattle)	PRRC2B	XP_005213498.1
Gallus gallus (chicken)	PRRC2B	XP_415459.3

Gene Ontology

The following PRRC2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRRC2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11310	XM_005172042.4 Latest version!	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa11311	XM_005172043.3 Latest version!	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa11312	XM_005172044.3 Latest version!	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa11313	XM_005172045.3 Latest version!	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa11314	XM_005172046.4 Latest version!	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
ODa11310	XM_005172042.3	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa11314	XM_005172046.3	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRRC2B ( XM_005172042.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172099.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

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CloneID	ODa11310
Clone ID Related Accession (Same CDS sequence)	XM_005172042.4 , XM_005172042.3
Accession Version	XM_005172042.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172042.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG AGTGTGTGGC GTCAGCAGCA 
CCAGTGAAAG CGTCAGCGTC TCGCAGCGGA GCCATCAAAC CACAGCCGCT CAAACTGGAG 
GAGGGAAAAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172099.1
CDS	244..6618
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKECVASAAPVKASASRSGAIKPQPLKLEEGKA

Target ORF information:

RefSeq Version	XM_005172042.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172042.4

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG AGTGTGTGGC GTCAGCAGCA 
CCAGTGAAAG CGTCAGCGTC TCGCAGCGGA GCCATCAAAC CACAGCCGCT CAAACTGGAG 
GAGGGAAAAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172042.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172099.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

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CloneID	ODa11310
Clone ID Related Accession (Same CDS sequence)	XM_005172042.4 , XM_005172042.3
Accession Version	XM_005172042.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG AGTGTGTGGC GTCAGCAGCA 
CCAGTGAAAG CGTCAGCGTC TCGCAGCGGA GCCATCAAAC CACAGCCGCT CAAACTGGAG 
GAGGGAAAAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172099.1
CDS	248..6622
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKECVASAAPVKASASRSGAIKPQPLKLEEGKA

Target ORF information:

RefSeq Version	XM_005172042.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172042.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG AGTGTGTGGC GTCAGCAGCA 
CCAGTGAAAG CGTCAGCGTC TCGCAGCGGA GCCATCAAAC CACAGCCGCT CAAACTGGAG 
GAGGGAAAAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172043.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172100.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	ODa11311
Clone ID Related Accession (Same CDS sequence)	XM_005172043.3
Accession Version	XM_005172043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172043.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGAAGGCCA GTGTGAACAG TGAGTCTGCA 
GAGGAGCAGG ATGAGCCCCA GCGGTCGGCT CCTCCTCGAG GGAAGTTCGT CTCAGCAGAC 
GTCTCGGCGG TGGAGCGAGC CCGTCGGCGC CGCGAGGAGG AGGAGCGCAG GGCTCGAGAG 
GAGAGACTGG CGGCCTGCGC TGAGAAACTC AAGAAACTGG ATGAGAAGTT TGGCAAAACG 
GAGAAACCTG CTCGCTCTGG AGAAGCAGAC GGCAAGGAGC TGGCGCAGTC TCCCGCTCGC 
AGATCCGCAA AACCCCAGCA GGATGGCTGG CAGTACGGCT CCAAAGAAGT GACGGACACG 
CCAGCGGAGT CAGCCTCCAG TCAGGATTAT AAAGATGAAG GCTGTAACTT CCACAATAAT 
GATGATGAGG ATGATGATGG TGGCGAGTCC ACGTCTCCTG TGCCGGAGTA CAGCCGACAT 
CAGAAGCCTG TGCCGCCACG CTTCCAGAAG CAGCAGCAGC AGCAGGAGCA GGTGTATAAG 
CTGGCGTCGT GGCAGCAGTC GGCTCACCCA GCTCAGCCCA GCTCTGCTCA CCCGCAGCGG 
GGCTTCTACC CTCCTCATGT GCTGGGCTTC GACCCACGCT GGATGATGAT GCCACCATAC 
ATGGACCCCC GGCTGGCACA GGGCTGCTCT CCTGTGGACT ACTACCCTCC TGGAGTCCAC 
TCATCAGGTT TGGTGAAGCC TGTAATTCAG CCGGATCACC TGAACAGTCC TGGCTCCACC 
TCGGATGAAG GCTGCCACCC CAGCATGCAT CAGGAGAGGA GAGCGCCGTC CACCGAGCCC 
TATCAAGTGT GGAACCAGGA CAGCTACCCC TCAGCGCGCA GTTTCACCCC GCCGTATCAG 
CGGACGCATG AGAACGGGGA GAGAGCTCCA GCGGACGACA GGAGCGACAG ATCCACATCC 
AGACACGACT CTTATGAAGA GCACAGACAC GAGCGCACGG ACAGCCCAGC CGAGGAAACA 
ACACACCAGG GTTTCCGGCA GGGCAGAGTG TCAGACGGGT CCCAGCGAGA ACCCATGATG 
CTCAGCAGGA CTCATGATGA TGCTCAACAC CAGAAAGAGG AGCCCTACGA CAGCAAGGAG 
AAAAGCTATG AGGCTGATTT CTGGAGGCGA GACGTGCGGA AGGAGAGCAG CGGTCAGACG 
CAGTGGTCCG ATCACGGCTC CAGCAGCAGC AGCAGCATCA GTCAGCCCTC CGAGACCAGC 
GGCAGAACCC TGCGCAGAAC CGGACCCATC AAAAAACCTG TGCTCAAGCC TCTGAAGGTG 
GAGGATAAAG AGAATGAGAA GCCCAAAGCG GAGCCTGAGG AGAAGCCGGT CCCCTACAGG 
CTGGAGAAGG AGGTGATCAC CAATGTGTAC GACCTGAAGA AAGACGCTCC GCACGTTTCA 
AACAGACACT CCAGCAGCGT CGAAGACAAA CAAGCAGACG CTCTGAAGAT GGAGAAAACC 
AGCGCGTTAT CTGAAGAGCT CCACAAGGAG AGCTGCTGGG AGAAATCCCA ATCCGACTGC 
TCCGACAGCA GAGATCCGAC CGCTCCGAGG CGCAACAACT GGATCTTCAT TGATGAGGAG 
CAGGCGTTCG CTGGAGCACG CGGTCGAGGA CGGGGACGTG GCTTTCGGGA GTTTAATTCT 
CGCGGAGGCG GAGCTCGTGG AGGACGCAGC GACACTAATA GAGGAGCGTA CAATAATAAC 
AACAACAACA CGGGCGCTCA GAGACTCACA CGAGGACGCG GAAACCGAGT GTTTAGAGGG 
GACGATCTGC AGAGGGGAAA ACCGCGCAGA CGAAACGTCA GCGAAACGCA CAGCGAAACC 
TCCGAGTACG AAGAGCAGCC CAAACGTCCT CGGCAGAAGG TGTCGGAGAA CGGCGGAGAA 
GCGTCAGGAG AGGTTAAAAA AGCCGACAGG GAATCCTGGA GATCCAATAA AGTCTACACT 
GACGATCAAA GCGAGAAACC CAAATCTAGA GTGTTCGGGC GTTCGTTCCC GCCGCGCCTC 
AATGCCGCGT ATAATCGCGG ATTCACCGGA ACCAGAGATA TTTCAACGTG GAGAGGAAGA 
GGAACGCAAT TCGGGAGTTC CTCCAGTCAG GAAAACGGCT ACAATTTCGT TGCGGACTCT 
TATTCGAAGC GCACCGAGCC GCTGAAGTAT CCTGCTAAAT TCACGGGCAC GTTTGCGGAG 
AACGGCGTGG AGGATCGTGA CGGAGGATAT TATGTTGATG ATAATCCGGA TAATCGTCCG 
CTGAGGAGAA GGCGTCCACC ACGGCAGGAT AAACCTCCAC GGTTCCGCCG TCTGCGTCAG 
GAGCGGGACG GAGCGGGAGC CTGGAGTAAT GATGACTACG TGAACGGAGA GTTTCCCAAT 
CAGTGGCCGA GCAGAGCGAA GAGTGCGGAG GAGCACTGGC AGAATCACTA CCCTGGAGGA 
CGCTCGCAGT CACAGGAGAT GACGGCTCAG AGTCAGGGTG AGGACTGGGA AACGGGTTCA 
GACAACAGTG ATTTCGGTGA CTGGCGGGAG AAGCGAGGGC CGCACGGGGA TCCGCTGACA 
GACGCGGCTC TCGGAGATCC GGGATCTGAG AAGAGAGAAC TCTCCAAACG CAGCTTTTCC 
AGCCAGCGGC CGCTGGACCG ACAGAACCGC AAGAATGACG TGTCTGTGAT GGAGAACAGC 
AAGATGGGCC GTCCGGCAGA TGCAGGATCC AGAAACGACA GCTGGCAGAA TGGAGTCGCG 
TCCGGCAGCA AACGATCTCC AGAAGAGTCT GTCAGCGGTC TGAGCTCTGC GTCGGTGTAC 
GGTGTGGAGC AGAACGAAGA CCGCAGCACG CCTGAGCCCA CAGGGAAGAT CATAGAGAAA 
GAGTTGAAGC CCAGAAACAT GAAGGCGGAC ATGACTGAAC CGCTGTCCCA GTACGACCTC 
AACACTTACT CGATTGAGAG TGACTCTGGA GCGTCGGTTC CCAGTCCAGA GGTTTTCCAG 
GATTCTCTGT CTAAAAAGCA GCGCCGTCCG CAGGAGGACG AGCGCAGGAG GAAGGAGCAG 
GGCCCTCCGA GTTCAGTAAA GAGCAGAACA ATTGCTTCAA AGATGCCTCC GCGGTTTGCC 
AAGAAGCAGG GCAGCATGTC CATGGAGCAG CCGGAGGAGA CGCTCACCGC AAACAACCTG 
GGAACTGAGA TCTGGGAGAC CAACAGCACA GCTCTGACTG TGCAGTCGTC AGGAGGCGAT 
TCCTGGACTA AACAGGTTTC CTACACGGGG AGCGAACCCA ACTCAGAGGA TTCAGATGCG 
GGACCGGAGC AGAGTAAAGA GCACAAGCCT GGTCCGATCG GGAATGAGCG CTCGCTGAAG 
AACCGGAAAG GCTCAGAGGG TCTGGAGCGC CTGGAGGGAT CCATCACTCC CGTTAACGGC 
GTGGATATTC ACGTGGAAAA CGTCATTCCT GTGCCGCCCA TCGAGTTCGG CGTCAATGCG 
AAAGATTCAG ACTTCAGCCT TCCTGCAGGA TCTGCAGCCG TGCCCGTCTC CAACCCCGTC 
TCCAAACTGC AGGACGCCCT CGCCGGGAAC GCGGGCCTGA CTCAAGCCAT CCCGATGCTG 
CGCAGAGATC ACCTGCAGCC CGCCATTACC CTGAACCCCA TCAGCTTCCC CTCCGCTGAC 
CTCACGCTCA AGATGGAGTC CGCCAGGAAG GCGTGGGAGA ACTCTCAGGC TGTGCCGGAG 
CAGGGGTCTC CAGGCTCCGG CTCTTCAGCT CAGCCGGTCT GCAGTGTGGG CTCCTCCAGC 
GTCAGCTACA GCTCCTTCTC CATGCCACCC ATGCCTGTGG CGTCTGTGGC CCCGTCCGTC 
TCCATGCAGG GCAGCCACAT CCCTCCTCTG TATCTGGATG GTCATGTGTT TCCCAGTCAG 
CCTCGTCTGG TGCCGCCGCT CACCCAGCAG CCCAGCTACC AGCAGGCGAC CCCTCAGCAG 
ATCCCCATCT CTCTGCACAC GTCTCTGCAG GCTCAGGCTC AGCTGGGTCT GCGTGGAGCT 
CTGCCCGTCT CTCAGTCACA AGAGATCTTC AGCTCCATCC CTCCATTCAG GTCTCAGGTG 
TACATGCACC CTAACCTGTC CCAGGCGAGC CCCATGGTGC TGTCGGGTGG AGCTGCTCTG 
AAAGGGCCGT ACTCCGCCTT CCCCGGCCTG CAGCCCTCAG ACCTGGTCAA ATCTCAGTCC 
GGATCCCACT ACCAGCCCAT CAACGGCAGC ACGCCACTGG TGTACGACCA GCCCACAGGC 
ATGAGCACCT CACAGCTGAT GGACTCGCAG CTCATTCAGG TGACGATGCC GATGCCAGGC 
TCTCAGCTGC GCTATGGTTC AGCCCAGCAG CACCTCATCC TCCCGCAGTC CATCCAGCTC 
CAGCAGAACC AGAATCTGTC TGTGGGAGCT CCACGCCGCA TGATGCCCCC AGGGTCACAG 
CCAGTGCTAA GCGGCAGCAG AGAGGTTTCA CAGATGGACA TGAAAGGCTT TCAGTTCTCA 
GATAAACCCA GCCACTCTCC TGGAATACCC AGCGGCTCAT ACAGGCCCGG CTCTGCCAGC 
CCCAGTGGGA AAGCGTCGGG TGCTCCAGCG GTGGCGTCTC TTGCAGGACA CTACACACAG 
CAGGTGTCGG GTCCTCAGGG CAGTATGGTG ATGCACATGC GTCCCCCCAG CAGCGGGCCC 
TTCCCCACCC CCATCCAGCG GCCCATCATG CAGGTCAACA AGCCCGTGAT CATCCGCTCC 
CCGCCGTACC CCGGCCCAAA CACCGGCCCC GCACACACAC ACGCACACAC ACACAACCCT 
GAGGGCCCCG GCAAGGGCCC GGAGGACGGC CTGAAGGAGT GTGTGGCGTC AGCAGCACCA 
GTGAAAGCGT CAGCGTCTCG CAGCGGAGCC ATCAAACCAC AGCCGCTCAA ACTGGAGGAG 
GGAAAAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172100.1
CDS	248..6619
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKECVASAAPVKASASRSGAIKPQPLKLEEGKA

Target ORF information:

RefSeq Version	XM_005172043.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172043.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGAAGGCCA GTGTGAACAG TGAGTCTGCA 
GAGGAGCAGG ATGAGCCCCA GCGGTCGGCT CCTCCTCGAG GGAAGTTCGT CTCAGCAGAC 
GTCTCGGCGG TGGAGCGAGC CCGTCGGCGC CGCGAGGAGG AGGAGCGCAG GGCTCGAGAG 
GAGAGACTGG CGGCCTGCGC TGAGAAACTC AAGAAACTGG ATGAGAAGTT TGGCAAAACG 
GAGAAACCTG CTCGCTCTGG AGAAGCAGAC GGCAAGGAGC TGGCGCAGTC TCCCGCTCGC 
AGATCCGCAA AACCCCAGCA GGATGGCTGG CAGTACGGCT CCAAAGAAGT GACGGACACG 
CCAGCGGAGT CAGCCTCCAG TCAGGATTAT AAAGATGAAG GCTGTAACTT CCACAATAAT 
GATGATGAGG ATGATGATGG TGGCGAGTCC ACGTCTCCTG TGCCGGAGTA CAGCCGACAT 
CAGAAGCCTG TGCCGCCACG CTTCCAGAAG CAGCAGCAGC AGCAGGAGCA GGTGTATAAG 
CTGGCGTCGT GGCAGCAGTC GGCTCACCCA GCTCAGCCCA GCTCTGCTCA CCCGCAGCGG 
GGCTTCTACC CTCCTCATGT GCTGGGCTTC GACCCACGCT GGATGATGAT GCCACCATAC 
ATGGACCCCC GGCTGGCACA GGGCTGCTCT CCTGTGGACT ACTACCCTCC TGGAGTCCAC 
TCATCAGGTT TGGTGAAGCC TGTAATTCAG CCGGATCACC TGAACAGTCC TGGCTCCACC 
TCGGATGAAG GCTGCCACCC CAGCATGCAT CAGGAGAGGA GAGCGCCGTC CACCGAGCCC 
TATCAAGTGT GGAACCAGGA CAGCTACCCC TCAGCGCGCA GTTTCACCCC GCCGTATCAG 
CGGACGCATG AGAACGGGGA GAGAGCTCCA GCGGACGACA GGAGCGACAG ATCCACATCC 
AGACACGACT CTTATGAAGA GCACAGACAC GAGCGCACGG ACAGCCCAGC CGAGGAAACA 
ACACACCAGG GTTTCCGGCA GGGCAGAGTG TCAGACGGGT CCCAGCGAGA ACCCATGATG 
CTCAGCAGGA CTCATGATGA TGCTCAACAC CAGAAAGAGG AGCCCTACGA CAGCAAGGAG 
AAAAGCTATG AGGCTGATTT CTGGAGGCGA GACGTGCGGA AGGAGAGCAG CGGTCAGACG 
CAGTGGTCCG ATCACGGCTC CAGCAGCAGC AGCAGCATCA GTCAGCCCTC CGAGACCAGC 
GGCAGAACCC TGCGCAGAAC CGGACCCATC AAAAAACCTG TGCTCAAGCC TCTGAAGGTG 
GAGGATAAAG AGAATGAGAA GCCCAAAGCG GAGCCTGAGG AGAAGCCGGT CCCCTACAGG 
CTGGAGAAGG AGGTGATCAC CAATGTGTAC GACCTGAAGA AAGACGCTCC GCACGTTTCA 
AACAGACACT CCAGCAGCGT CGAAGACAAA CAAGCAGACG CTCTGAAGAT GGAGAAAACC 
AGCGCGTTAT CTGAAGAGCT CCACAAGGAG AGCTGCTGGG AGAAATCCCA ATCCGACTGC 
TCCGACAGCA GAGATCCGAC CGCTCCGAGG CGCAACAACT GGATCTTCAT TGATGAGGAG 
CAGGCGTTCG CTGGAGCACG CGGTCGAGGA CGGGGACGTG GCTTTCGGGA GTTTAATTCT 
CGCGGAGGCG GAGCTCGTGG AGGACGCAGC GACACTAATA GAGGAGCGTA CAATAATAAC 
AACAACAACA CGGGCGCTCA GAGACTCACA CGAGGACGCG GAAACCGAGT GTTTAGAGGG 
GACGATCTGC AGAGGGGAAA ACCGCGCAGA CGAAACGTCA GCGAAACGCA CAGCGAAACC 
TCCGAGTACG AAGAGCAGCC CAAACGTCCT CGGCAGAAGG TGTCGGAGAA CGGCGGAGAA 
GCGTCAGGAG AGGTTAAAAA AGCCGACAGG GAATCCTGGA GATCCAATAA AGTCTACACT 
GACGATCAAA GCGAGAAACC CAAATCTAGA GTGTTCGGGC GTTCGTTCCC GCCGCGCCTC 
AATGCCGCGT ATAATCGCGG ATTCACCGGA ACCAGAGATA TTTCAACGTG GAGAGGAAGA 
GGAACGCAAT TCGGGAGTTC CTCCAGTCAG GAAAACGGCT ACAATTTCGT TGCGGACTCT 
TATTCGAAGC GCACCGAGCC GCTGAAGTAT CCTGCTAAAT TCACGGGCAC GTTTGCGGAG 
AACGGCGTGG AGGATCGTGA CGGAGGATAT TATGTTGATG ATAATCCGGA TAATCGTCCG 
CTGAGGAGAA GGCGTCCACC ACGGCAGGAT AAACCTCCAC GGTTCCGCCG TCTGCGTCAG 
GAGCGGGACG GAGCGGGAGC CTGGAGTAAT GATGACTACG TGAACGGAGA GTTTCCCAAT 
CAGTGGCCGA GCAGAGCGAA GAGTGCGGAG GAGCACTGGC AGAATCACTA CCCTGGAGGA 
CGCTCGCAGT CACAGGAGAT GACGGCTCAG AGTCAGGGTG AGGACTGGGA AACGGGTTCA 
GACAACAGTG ATTTCGGTGA CTGGCGGGAG AAGCGAGGGC CGCACGGGGA TCCGCTGACA 
GACGCGGCTC TCGGAGATCC GGGATCTGAG AAGAGAGAAC TCTCCAAACG CAGCTTTTCC 
AGCCAGCGGC CGCTGGACCG ACAGAACCGC AAGAATGACG TGTCTGTGAT GGAGAACAGC 
AAGATGGGCC GTCCGGCAGA TGCAGGATCC AGAAACGACA GCTGGCAGAA TGGAGTCGCG 
TCCGGCAGCA AACGATCTCC AGAAGAGTCT GTCAGCGGTC TGAGCTCTGC GTCGGTGTAC 
GGTGTGGAGC AGAACGAAGA CCGCAGCACG CCTGAGCCCA CAGGGAAGAT CATAGAGAAA 
GAGTTGAAGC CCAGAAACAT GAAGGCGGAC ATGACTGAAC CGCTGTCCCA GTACGACCTC 
AACACTTACT CGATTGAGAG TGACTCTGGA GCGTCGGTTC CCAGTCCAGA GGTTTTCCAG 
GATTCTCTGT CTAAAAAGCA GCGCCGTCCG CAGGAGGACG AGCGCAGGAG GAAGGAGCAG 
GGCCCTCCGA GTTCAGTAAA GAGCAGAACA ATTGCTTCAA AGATGCCTCC GCGGTTTGCC 
AAGAAGCAGG GCAGCATGTC CATGGAGCAG CCGGAGGAGA CGCTCACCGC AAACAACCTG 
GGAACTGAGA TCTGGGAGAC CAACAGCACA GCTCTGACTG TGCAGTCGTC AGGAGGCGAT 
TCCTGGACTA AACAGGTTTC CTACACGGGG AGCGAACCCA ACTCAGAGGA TTCAGATGCG 
GGACCGGAGC AGAGTAAAGA GCACAAGCCT GGTCCGATCG GGAATGAGCG CTCGCTGAAG 
AACCGGAAAG GCTCAGAGGG TCTGGAGCGC CTGGAGGGAT CCATCACTCC CGTTAACGGC 
GTGGATATTC ACGTGGAAAA CGTCATTCCT GTGCCGCCCA TCGAGTTCGG CGTCAATGCG 
AAAGATTCAG ACTTCAGCCT TCCTGCAGGA TCTGCAGCCG TGCCCGTCTC CAACCCCGTC 
TCCAAACTGC AGGACGCCCT CGCCGGGAAC GCGGGCCTGA CTCAAGCCAT CCCGATGCTG 
CGCAGAGATC ACCTGCAGCC CGCCATTACC CTGAACCCCA TCAGCTTCCC CTCCGCTGAC 
CTCACGCTCA AGATGGAGTC CGCCAGGAAG GCGTGGGAGA ACTCTCAGGC TGTGCCGGAG 
CAGGGGTCTC CAGGCTCCGG CTCTTCAGCT CAGCCGGTCT GCAGTGTGGG CTCCTCCAGC 
GTCAGCTACA GCTCCTTCTC CATGCCACCC ATGCCTGTGG CGTCTGTGGC CCCGTCCGTC 
TCCATGCAGG GCAGCCACAT CCCTCCTCTG TATCTGGATG GTCATGTGTT TCCCAGTCAG 
CCTCGTCTGG TGCCGCCGCT CACCCAGCAG CCCAGCTACC AGCAGGCGAC CCCTCAGCAG 
ATCCCCATCT CTCTGCACAC GTCTCTGCAG GCTCAGGCTC AGCTGGGTCT GCGTGGAGCT 
CTGCCCGTCT CTCAGTCACA AGAGATCTTC AGCTCCATCC CTCCATTCAG GTCTCAGGTG 
TACATGCACC CTAACCTGTC CCAGGCGAGC CCCATGGTGC TGTCGGGTGG AGCTGCTCTG 
AAAGGGCCGT ACTCCGCCTT CCCCGGCCTG CAGCCCTCAG ACCTGGTCAA ATCTCAGTCC 
GGATCCCACT ACCAGCCCAT CAACGGCAGC ACGCCACTGG TGTACGACCA GCCCACAGGC 
ATGAGCACCT CACAGCTGAT GGACTCGCAG CTCATTCAGG TGACGATGCC GATGCCAGGC 
TCTCAGCTGC GCTATGGTTC AGCCCAGCAG CACCTCATCC TCCCGCAGTC CATCCAGCTC 
CAGCAGAACC AGAATCTGTC TGTGGGAGCT CCACGCCGCA TGATGCCCCC AGGGTCACAG 
CCAGTGCTAA GCGGCAGCAG AGAGGTTTCA CAGATGGACA TGAAAGGCTT TCAGTTCTCA 
GATAAACCCA GCCACTCTCC TGGAATACCC AGCGGCTCAT ACAGGCCCGG CTCTGCCAGC 
CCCAGTGGGA AAGCGTCGGG TGCTCCAGCG GTGGCGTCTC TTGCAGGACA CTACACACAG 
CAGGTGTCGG GTCCTCAGGG CAGTATGGTG ATGCACATGC GTCCCCCCAG CAGCGGGCCC 
TTCCCCACCC CCATCCAGCG GCCCATCATG CAGGTCAACA AGCCCGTGAT CATCCGCTCC 
CCGCCGTACC CCGGCCCAAA CACCGGCCCC GCACACACAC ACGCACACAC ACACAACCCT 
GAGGGCCCCG GCAAGGGCCC GGAGGACGGC CTGAAGGAGT GTGTGGCGTC AGCAGCACCA 
GTGAAAGCGT CAGCGTCTCG CAGCGGAGCC ATCAAACCAC AGCCGCTCAA ACTGGAGGAG 
GGAAAAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172044.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172101.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

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CloneID	ODa11312
Clone ID Related Accession (Same CDS sequence)	XM_005172044.3
Accession Version	XM_005172044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGT GACGGACACG 
CCAGCGGAGT CAGCCTCCAG TCAGGATTAT AAAGATGAAG GCTGTAACTT CCACAATAAT 
GATGATGAGG ATGATGATGG TGGCGAGTCC ACGTCTCCTG TGCCGGAGTA CAGCCGACAT 
CAGAAGCCTG TGCCGCCACG CTTCCAGAAG CAGCAGCAGC AGCAGGAGCA GGTGTATAAG 
CTGGCGTCGT GGCAGCAGTC GGCTCACCCA GCTCAGCCCA GCTCTGCTCA CCCGCAGCGG 
GGCTTCTACC CTCCTCATGT GCTGGGCTTC GACCCACGCT GGATGATGAT GCCACCATAC 
ATGGACCCCC GGCTGGCACA GGGCTGCTCT CCTGTGGACT ACTACCCTCC TGGAGTCCAC 
TCATCAGGTT TGGTGAAGCC TGTAATTCAG CCGGATCACC TGAACAGTCC TGGCTCCACC 
TCGGATGAAG GCTGCCACCC CAGCATGCAT CAGGAGAGGA GAGCGCCGTC CACCGAGCCC 
TATCAAGTGT GGAACCAGGA CAGCTACCCC TCAGCGCGCA GTTTCACCCC GCCGTATCAG 
CGGACGCATG AGAACGGGGA GAGAGCTCCA GCGGACGACA GGAGCGACAG ATCCACATCC 
AGACACGACT CTTATGAAGA GCACAGACAC GAGCGCACGG ACAGCCCAGC CGAGGAAACA 
ACACACCAGG GTTTCCGGCA GGGCAGAGTG TCAGACGGGT CCCAGCGAGA ACCCATGATG 
CTCAGCAGGA CTCATGATGA TGCTCAACAC CAGAAAGAGG AGCCCTACGA CAGCAAGGAG 
AAAAGCTATG AGGCTGATTT CTGGAGGCGA GACGTGCGGA AGGAGAGCAG CGGTCAGACG 
CAGTGGTCCG ATCACGGCTC CAGCAGCAGC AGCAGCATCA GTCAGCCCTC CGAGACCAGC 
GGCAGAACCC TGCGCAGAAC CGGACCCATC AAAAAACCTG TGCTCAAGCC TCTGAAGGTG 
GAGGATAAAG AGAATGAGAA GCCCAAAGCG GAGCCTGAGG AGAAGCCGGT CCCCTACAGG 
CTGGAGAAGG AGGTGATCAC CAATGTGTAC GACCTGAAGA AAGACGCTCC GCACGTTTCA 
AACAGACACT CCAGCAGCGT CGAAGACAAA CAAGCAGACG CTCTGAAGAT GGAGAAAACC 
AGCGCGTTAT CTGAAGAGCT CCACAAGGAG AGCTGCTGGG AGAAATCCCA ATCCGACTGC 
TCCGACAGCA GAGATCCGAC CGCTCCGAGG CGCAACAACT GGATCTTCAT TGATGAGGAG 
CAGGCGTTCG CTGGAGCACG CGGTCGAGGA CGGGGACGTG GCTTTCGGGA GTTTAATTCT 
CGCGGAGGCG GAGCTCGTGG AGGACGCAGC GACACTAATA GAGGAGCGTA CAATAATAAC 
AACAACAACA CGGGCGCTCA GAGACTCACA CGAGGACGCG GAAACCGAGT GTTTAGAGGG 
GACGATCTGC AGAGGGGAAA ACCGCGCAGA CGAAACGTCA GCGAAACGCA CAGCGAAACC 
TCCGAGTACG AAGAGCAGCC CAAACGTCCT CGGCAGAAGG TGTCGGAGAA CGGCGGAGAA 
GCGTCAGGAG AGGTTAAAAA AGCCGACAGG GAATCCTGGA GATCCAATAA AGTCTACACT 
GACGATCAAA GCGAGAAACC CAAATCTAGA GTGTTCGGGC GTTCGTTCCC GCCGCGCCTC 
AATGCCGCGT ATAATCGCGG ATTCACCGGA ACCAGAGATA TTTCAACGTG GAGAGGAAGA 
GGAACGCAAT TCGGGAGTTC CTCCAGTCAG GAAAACGGCT ACAATTTCGT TGCGGACTCT 
TATTCGAAGC GCACCGAGCC GCTGAAGTAT CCTGCTAAAT TCACGGGCAC GTTTGCGGAG 
AACGGCGTGG AGGATCGTGA CGGAGGATAT TATGTTGATG ATAATCCGGA TAATCGTCCG 
CTGAGGAGAA GGCGTCCACC ACGGCAGGAT AAACCTCCAC GGTTCCGCCG TCTGCGTCAG 
GAGCGGGACG GAGCGGGAGC CTGGAGTAAT GATGACTACG TGAACGGAGA GTTTCCCAAT 
CAGTGGCCGA GCAGAGCGAA GAGTGCGGAG GAGCACTGGC AGAATCACTA CCCTGGAGGA 
CGCTCGCAGT CACAGGAGAT GACGGCTCAG AGTCAGGGTG AGGACTGGGA AACGGGTTCA 
GACAACAGTG ATTTCGGTGA CTGGCGGGAG AAGCGAGGGC CGCACGGGGA TCCGCTGACA 
GACGCGGCTC TCGGAGATCC GGGATCTGAG AAGAGAGAAC TCTCCAAACG CAGCTTTTCC 
AGCCAGCGGC CGCTGGACCG ACAGAACCGC AAGAATGACG TGTCTGTGAT GGAGAACAGC 
AAGATGGGCC GTCCGGCAGA TGCAGGATCC AGAAACGACA GCTGGCAGAA TGGAGTCGCG 
TCCGGCAGCA AACGATCTCC AGAAGAGTCT GTCAGCGGTC TGAGCTCTGC GTCGGTGTAC 
GGTGTGGAGC AGAACGAAGA CCGCAGCACG CCTGAGCCCA CAGGGAAGAT CATAGAGAAA 
GAGTTGAAGC CCAGAAACAT GAAGGCGGAC ATGACTGAAC CGCTGTCCCA GTACGACCTC 
AACACTTACT CGATTGAGAG TGACTCTGGA GCGTCGGTTC CCAGTCCAGA GGTTTTCCAG 
GATTCTCTGT CTAAAAAGCA GCGCCGTCCG CAGGAGGACG AGCGCAGGAG GAAGGAGCAG 
GGCCCTCCGA GTTCAGTAAA GAGCAGAACA ATTGCTTCAA AGATGCCTCC GCGGTTTGCC 
AAGAAGCAGG GCAGCATGTC CATGGAGCAG CCGGAGGAGA CGCTCACCGC AAACAACCTG 
GGAACTGAGA TCTGGGAGAC CAACAGCACA GCTCTGACTG TGCAGTCGTC AGGAGGCGAT 
TCCTGGACTA AACAGGTTTC CTACACGGGG AGCGAACCCA ACTCAGAGGA TTCAGATGCG 
GGACCGGAGC AGAGTAAAGA GCACAAGCCT GGTCCGATCG GGAATGAGCG CTCGCTGAAG 
AACCGGAAAG GCTCAGAGGG TCTGGAGCGC CTGGAGGGAT CCATCACTCC CGTTAACGGC 
GTGGATATTC ACGTGGAAAA CGTCATTCCT GTGCCGCCCA TCGAGTTCGG CGTCAATGCG 
AAAGATTCAG ACTTCAGCCT TCCTGCAGGA TCTGCAGCCG TGCCCGTCTC CAACCCCGTC 
TCCAAACTGC AGGACGCCCT CGCCGGGAAC GCGGGCCTGA CTCAAGCCAT CCCGATGCTG 
CGCAGAGATC ACCTGCAGCC CGCCATTACC CTGAACCCCA TCAGCTTCCC CTCCGCTGAC 
CTCACGCTCA AGATGGAGTC CGCCAGGAAG GCGTGGGAGA ACTCTCAGGC TGTGCCGGAG 
CAGGGGTCTC CAGGCTCCGG CTCTTCAGCT CAGCCGGTCT GCAGTGTGGG CTCCTCCAGC 
GTCAGCTACA GCTCCTTCTC CATGCCACCC ATGCCTGTGG CGTCTGTGGC CCCGTCCGTC 
TCCATGCAGG GCAGCCACAT CCCTCCTCTG TATCTGGATG GTCATGTGTT TCCCAGTCAG 
CCTCGTCTGG TGCCGCCGCT CACCCAGCAG CCCAGCTACC AGCAGGCGAC CCCTCAGCAG 
ATCCCCATCT CTCTGCACAC GTCTCTGCAG GCTCAGGCTC AGCTGGGTCT GCGTGGAGCT 
CTGCCCGTCT CTCAGTCACA AGAGATCTTC AGCTCCATCC CTCCATTCAG GTCTCAGGTG 
TACATGCACC CTAACCTGTC CCAGGCGAGC CCCATGGTGC TGTCGGGTGG AGCTGCTCTG 
AAAGGGCCGT ACTCCGCCTT CCCCGGCCTG CAGCCCTCAG ACCTGGTCAA ATCTCAGTCC 
GGATCCCACT ACCAGCCCAT CAACGGCAGC ACGCCACTGG TGTACGACCA GCCCACAGGC 
ATGAGCACCT CACAGCTGAT GGACTCGCAG CTCATTCAGG TGACGATGCC GATGCCAGGC 
TCTCAGCTGC GCTATGGTTC AGCCCAGCAG CACCTCATCC TCCCGCAGTC CATCCAGCTC 
CAGCAGAACC AGAATCTGTC TGTGGGAGCT CCACGCCGCA TGATGCCCCC AGGGTCACAG 
CCAGTGCTAA GCGGCAGCAG AGAGGTTTCA CAGATGGACA TGAAAGGCTT TCAGTTCTCA 
GATAAACCCA GCCACTCTCC TGGAATACCC AGCGGCTCAT ACAGGCCCGG CTCTGCCAGC 
CCCAGTGGGA AAGCGTCGGG TGCTCCAGCG GTGGCGTCTC TTGCAGGACA CTACACACAG 
CAGGTGTCGG GTCCTCAGGG CAGTATGGTG ATGCACATGC GTCCCCCCAG CAGCGGGCCC 
TTCCCCACCC CCATCCAGCG GCCCATCATG CAGGTCAACA AGCCCGTGAT CATCCGCTCC 
CCGCCGTACC CCGGCCCAAA CACCGGCCCC GCACACACAC ACGCACACAC ACACAACCCT 
GAGGGCCCCG GCAAGGGCCC GGAGGACGGC CTGAAGGAGT GTGTGGCGTC AGCAGCACCA 
GTGAAAGCGT CAGCGTCTCG CAGCGGAGCC ATCAAACCAC AGCCGCTCAA ACTGGAGGAG 
GGAAAAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172101.1
CDS	248..6619
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKECVASAAPVKASASRSGAIKPQPLKLEEGKA

Target ORF information:

RefSeq Version	XM_005172044.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172044.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGT GACGGACACG 
CCAGCGGAGT CAGCCTCCAG TCAGGATTAT AAAGATGAAG GCTGTAACTT CCACAATAAT 
GATGATGAGG ATGATGATGG TGGCGAGTCC ACGTCTCCTG TGCCGGAGTA CAGCCGACAT 
CAGAAGCCTG TGCCGCCACG CTTCCAGAAG CAGCAGCAGC AGCAGGAGCA GGTGTATAAG 
CTGGCGTCGT GGCAGCAGTC GGCTCACCCA GCTCAGCCCA GCTCTGCTCA CCCGCAGCGG 
GGCTTCTACC CTCCTCATGT GCTGGGCTTC GACCCACGCT GGATGATGAT GCCACCATAC 
ATGGACCCCC GGCTGGCACA GGGCTGCTCT CCTGTGGACT ACTACCCTCC TGGAGTCCAC 
TCATCAGGTT TGGTGAAGCC TGTAATTCAG CCGGATCACC TGAACAGTCC TGGCTCCACC 
TCGGATGAAG GCTGCCACCC CAGCATGCAT CAGGAGAGGA GAGCGCCGTC CACCGAGCCC 
TATCAAGTGT GGAACCAGGA CAGCTACCCC TCAGCGCGCA GTTTCACCCC GCCGTATCAG 
CGGACGCATG AGAACGGGGA GAGAGCTCCA GCGGACGACA GGAGCGACAG ATCCACATCC 
AGACACGACT CTTATGAAGA GCACAGACAC GAGCGCACGG ACAGCCCAGC CGAGGAAACA 
ACACACCAGG GTTTCCGGCA GGGCAGAGTG TCAGACGGGT CCCAGCGAGA ACCCATGATG 
CTCAGCAGGA CTCATGATGA TGCTCAACAC CAGAAAGAGG AGCCCTACGA CAGCAAGGAG 
AAAAGCTATG AGGCTGATTT CTGGAGGCGA GACGTGCGGA AGGAGAGCAG CGGTCAGACG 
CAGTGGTCCG ATCACGGCTC CAGCAGCAGC AGCAGCATCA GTCAGCCCTC CGAGACCAGC 
GGCAGAACCC TGCGCAGAAC CGGACCCATC AAAAAACCTG TGCTCAAGCC TCTGAAGGTG 
GAGGATAAAG AGAATGAGAA GCCCAAAGCG GAGCCTGAGG AGAAGCCGGT CCCCTACAGG 
CTGGAGAAGG AGGTGATCAC CAATGTGTAC GACCTGAAGA AAGACGCTCC GCACGTTTCA 
AACAGACACT CCAGCAGCGT CGAAGACAAA CAAGCAGACG CTCTGAAGAT GGAGAAAACC 
AGCGCGTTAT CTGAAGAGCT CCACAAGGAG AGCTGCTGGG AGAAATCCCA ATCCGACTGC 
TCCGACAGCA GAGATCCGAC CGCTCCGAGG CGCAACAACT GGATCTTCAT TGATGAGGAG 
CAGGCGTTCG CTGGAGCACG CGGTCGAGGA CGGGGACGTG GCTTTCGGGA GTTTAATTCT 
CGCGGAGGCG GAGCTCGTGG AGGACGCAGC GACACTAATA GAGGAGCGTA CAATAATAAC 
AACAACAACA CGGGCGCTCA GAGACTCACA CGAGGACGCG GAAACCGAGT GTTTAGAGGG 
GACGATCTGC AGAGGGGAAA ACCGCGCAGA CGAAACGTCA GCGAAACGCA CAGCGAAACC 
TCCGAGTACG AAGAGCAGCC CAAACGTCCT CGGCAGAAGG TGTCGGAGAA CGGCGGAGAA 
GCGTCAGGAG AGGTTAAAAA AGCCGACAGG GAATCCTGGA GATCCAATAA AGTCTACACT 
GACGATCAAA GCGAGAAACC CAAATCTAGA GTGTTCGGGC GTTCGTTCCC GCCGCGCCTC 
AATGCCGCGT ATAATCGCGG ATTCACCGGA ACCAGAGATA TTTCAACGTG GAGAGGAAGA 
GGAACGCAAT TCGGGAGTTC CTCCAGTCAG GAAAACGGCT ACAATTTCGT TGCGGACTCT 
TATTCGAAGC GCACCGAGCC GCTGAAGTAT CCTGCTAAAT TCACGGGCAC GTTTGCGGAG 
AACGGCGTGG AGGATCGTGA CGGAGGATAT TATGTTGATG ATAATCCGGA TAATCGTCCG 
CTGAGGAGAA GGCGTCCACC ACGGCAGGAT AAACCTCCAC GGTTCCGCCG TCTGCGTCAG 
GAGCGGGACG GAGCGGGAGC CTGGAGTAAT GATGACTACG TGAACGGAGA GTTTCCCAAT 
CAGTGGCCGA GCAGAGCGAA GAGTGCGGAG GAGCACTGGC AGAATCACTA CCCTGGAGGA 
CGCTCGCAGT CACAGGAGAT GACGGCTCAG AGTCAGGGTG AGGACTGGGA AACGGGTTCA 
GACAACAGTG ATTTCGGTGA CTGGCGGGAG AAGCGAGGGC CGCACGGGGA TCCGCTGACA 
GACGCGGCTC TCGGAGATCC GGGATCTGAG AAGAGAGAAC TCTCCAAACG CAGCTTTTCC 
AGCCAGCGGC CGCTGGACCG ACAGAACCGC AAGAATGACG TGTCTGTGAT GGAGAACAGC 
AAGATGGGCC GTCCGGCAGA TGCAGGATCC AGAAACGACA GCTGGCAGAA TGGAGTCGCG 
TCCGGCAGCA AACGATCTCC AGAAGAGTCT GTCAGCGGTC TGAGCTCTGC GTCGGTGTAC 
GGTGTGGAGC AGAACGAAGA CCGCAGCACG CCTGAGCCCA CAGGGAAGAT CATAGAGAAA 
GAGTTGAAGC CCAGAAACAT GAAGGCGGAC ATGACTGAAC CGCTGTCCCA GTACGACCTC 
AACACTTACT CGATTGAGAG TGACTCTGGA GCGTCGGTTC CCAGTCCAGA GGTTTTCCAG 
GATTCTCTGT CTAAAAAGCA GCGCCGTCCG CAGGAGGACG AGCGCAGGAG GAAGGAGCAG 
GGCCCTCCGA GTTCAGTAAA GAGCAGAACA ATTGCTTCAA AGATGCCTCC GCGGTTTGCC 
AAGAAGCAGG GCAGCATGTC CATGGAGCAG CCGGAGGAGA CGCTCACCGC AAACAACCTG 
GGAACTGAGA TCTGGGAGAC CAACAGCACA GCTCTGACTG TGCAGTCGTC AGGAGGCGAT 
TCCTGGACTA AACAGGTTTC CTACACGGGG AGCGAACCCA ACTCAGAGGA TTCAGATGCG 
GGACCGGAGC AGAGTAAAGA GCACAAGCCT GGTCCGATCG GGAATGAGCG CTCGCTGAAG 
AACCGGAAAG GCTCAGAGGG TCTGGAGCGC CTGGAGGGAT CCATCACTCC CGTTAACGGC 
GTGGATATTC ACGTGGAAAA CGTCATTCCT GTGCCGCCCA TCGAGTTCGG CGTCAATGCG 
AAAGATTCAG ACTTCAGCCT TCCTGCAGGA TCTGCAGCCG TGCCCGTCTC CAACCCCGTC 
TCCAAACTGC AGGACGCCCT CGCCGGGAAC GCGGGCCTGA CTCAAGCCAT CCCGATGCTG 
CGCAGAGATC ACCTGCAGCC CGCCATTACC CTGAACCCCA TCAGCTTCCC CTCCGCTGAC 
CTCACGCTCA AGATGGAGTC CGCCAGGAAG GCGTGGGAGA ACTCTCAGGC TGTGCCGGAG 
CAGGGGTCTC CAGGCTCCGG CTCTTCAGCT CAGCCGGTCT GCAGTGTGGG CTCCTCCAGC 
GTCAGCTACA GCTCCTTCTC CATGCCACCC ATGCCTGTGG CGTCTGTGGC CCCGTCCGTC 
TCCATGCAGG GCAGCCACAT CCCTCCTCTG TATCTGGATG GTCATGTGTT TCCCAGTCAG 
CCTCGTCTGG TGCCGCCGCT CACCCAGCAG CCCAGCTACC AGCAGGCGAC CCCTCAGCAG 
ATCCCCATCT CTCTGCACAC GTCTCTGCAG GCTCAGGCTC AGCTGGGTCT GCGTGGAGCT 
CTGCCCGTCT CTCAGTCACA AGAGATCTTC AGCTCCATCC CTCCATTCAG GTCTCAGGTG 
TACATGCACC CTAACCTGTC CCAGGCGAGC CCCATGGTGC TGTCGGGTGG AGCTGCTCTG 
AAAGGGCCGT ACTCCGCCTT CCCCGGCCTG CAGCCCTCAG ACCTGGTCAA ATCTCAGTCC 
GGATCCCACT ACCAGCCCAT CAACGGCAGC ACGCCACTGG TGTACGACCA GCCCACAGGC 
ATGAGCACCT CACAGCTGAT GGACTCGCAG CTCATTCAGG TGACGATGCC GATGCCAGGC 
TCTCAGCTGC GCTATGGTTC AGCCCAGCAG CACCTCATCC TCCCGCAGTC CATCCAGCTC 
CAGCAGAACC AGAATCTGTC TGTGGGAGCT CCACGCCGCA TGATGCCCCC AGGGTCACAG 
CCAGTGCTAA GCGGCAGCAG AGAGGTTTCA CAGATGGACA TGAAAGGCTT TCAGTTCTCA 
GATAAACCCA GCCACTCTCC TGGAATACCC AGCGGCTCAT ACAGGCCCGG CTCTGCCAGC 
CCCAGTGGGA AAGCGTCGGG TGCTCCAGCG GTGGCGTCTC TTGCAGGACA CTACACACAG 
CAGGTGTCGG GTCCTCAGGG CAGTATGGTG ATGCACATGC GTCCCCCCAG CAGCGGGCCC 
TTCCCCACCC CCATCCAGCG GCCCATCATG CAGGTCAACA AGCCCGTGAT CATCCGCTCC 
CCGCCGTACC CCGGCCCAAA CACCGGCCCC GCACACACAC ACGCACACAC ACACAACCCT 
GAGGGCCCCG GCAAGGGCCC GGAGGACGGC CTGAAGGAGT GTGTGGCGTC AGCAGCACCA 
GTGAAAGCGT CAGCGTCTCG CAGCGGAGCC ATCAAACCAC AGCCGCTCAA ACTGGAGGAG 
GGAAAAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172045.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172102.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

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CloneID	ODa11313
Clone ID Related Accession (Same CDS sequence)	XM_005172045.3
Accession Version	XM_005172045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGT GTATAAGCTG 
GCGTCGTGGC AGCAGTCGGC TCACCCAGCT CAGCCCAGCT CTGCTCACCC GCAGCGGGGC 
TTCTACCCTC CTCATGTGCT GGGCTTCGAC CCACGCTGGA TGATGATGCC ACCATACATG 
GACCCCCGGC TGGCACAGGG CTGCTCTCCT GTGGACTACT ACCCTCCTGG AGTCCACTCA 
TCAGGTTTGG TGAAGCCTGT AATTCAGCCG GATCACCTGA ACAGTCCTGG CTCCACCTCG 
GATGAAGGCT GCCACCCCAG CATGCATCAG GAGAGGAGAG CGCCGTCCAC CGAGCCCTAT 
CAAGTGTGGA ACCAGGACAG CTACCCCTCA GCGCGCAGTT TCACCCCGCC GTATCAGCGG 
ACGCATGAGA ACGGGGAGAG AGCTCCAGCG GACGACAGGA GCGACAGATC CACATCCAGA 
CACGACTCTT ATGAAGAGCA CAGACACGAG CGCACGGACA GCCCAGCCGA GGAAACAACA 
CACCAGGGTT TCCGGCAGGG CAGAGTGTCA GACGGGTCCC AGCGAGAACC CATGATGCTC 
AGCAGGACTC ATGATGATGC TCAACACCAG AAAGAGGAGC CCTACGACAG CAAGGAGAAA 
AGCTATGAGG CTGATTTCTG GAGGCGAGAC GTGCGGAAGG AGAGCAGCGG TCAGACGCAG 
TGGTCCGATC ACGGCTCCAG CAGCAGCAGC AGCATCAGTC AGCCCTCCGA GACCAGCGGC 
AGAACCCTGC GCAGAACCGG ACCCATCAAA AAACCTGTGC TCAAGCCTCT GAAGGTGGAG 
GATAAAGAGA ATGAGAAGCC CAAAGCGGAG CCTGAGGAGA AGCCGGTCCC CTACAGGCTG 
GAGAAGGAGG TGATCACCAA TGTGTACGAC CTGAAGAAAG ACGCTCCGCA CGTTTCAAAC 
AGACACTCCA GCAGCGTCGA AGACAAACAA GCAGACGCTC TGAAGATGGA GAAAACCAGC 
GCGTTATCTG AAGAGCTCCA CAAGGAGAGC TGCTGGGAGA AATCCCAATC CGACTGCTCC 
GACAGCAGAG ATCCGACCGC TCCGAGGCGC AACAACTGGA TCTTCATTGA TGAGGAGCAG 
GCGTTCGCTG GAGCACGCGG TCGAGGACGG GGACGTGGCT TTCGGGAGTT TAATTCTCGC 
GGAGGCGGAG CTCGTGGAGG ACGCAGCGAC ACTAATAGAG GAGCGTACAA TAATAACAAC 
AACAACACGG GCGCTCAGAG ACTCACACGA GGACGCGGAA ACCGAGTGTT TAGAGGGGAC 
GATCTGCAGA GGGGAAAACC GCGCAGACGA AACGTCAGCG AAACGCACAG CGAAACCTCC 
GAGTACGAAG AGCAGCCCAA ACGTCCTCGG CAGAAGGTGT CGGAGAACGG CGGAGAAGCG 
TCAGGAGAGG TTAAAAAAGC CGACAGGGAA TCCTGGAGAT CCAATAAAGT CTACACTGAC 
GATCAAAGCG AGAAACCCAA ATCTAGAGTG TTCGGGCGTT CGTTCCCGCC GCGCCTCAAT 
GCCGCGTATA ATCGCGGATT CACCGGAACC AGAGATATTT CAACGTGGAG AGGAAGAGGA 
ACGCAATTCG GGAGTTCCTC CAGTCAGGAA AACGGCTACA ATTTCGTTGC GGACTCTTAT 
TCGAAGCGCA CCGAGCCGCT GAAGTATCCT GCTAAATTCA CGGGCACGTT TGCGGAGAAC 
GGCGTGGAGG ATCGTGACGG AGGATATTAT GTTGATGATA ATCCGGATAA TCGTCCGCTG 
AGGAGAAGGC GTCCACCACG GCAGGATAAA CCTCCACGGT TCCGCCGTCT GCGTCAGGAG 
CGGGACGGAG CGGGAGCCTG GAGTAATGAT GACTACGTGA ACGGAGAGTT TCCCAATCAG 
TGGCCGAGCA GAGCGAAGAG TGCGGAGGAG CACTGGCAGA ATCACTACCC TGGAGGACGC 
TCGCAGTCAC AGGAGATGAC GGCTCAGAGT CAGGGTGAGG ACTGGGAAAC GGGTTCAGAC 
AACAGTGATT TCGGTGACTG GCGGGAGAAG CGAGGGCCGC ACGGGGATCC GCTGACAGAC 
GCGGCTCTCG GAGATCCGGG ATCTGAGAAG AGAGAACTCT CCAAACGCAG CTTTTCCAGC 
CAGCGGCCGC TGGACCGACA GAACCGCAAG AATGACGTGT CTGTGATGGA GAACAGCAAG 
ATGGGCCGTC CGGCAGATGC AGGATCCAGA AACGACAGCT GGCAGAATGG AGTCGCGTCC 
GGCAGCAAAC GATCTCCAGA AGAGTCTGTC AGCGGTCTGA GCTCTGCGTC GGTGTACGGT 
GTGGAGCAGA ACGAAGACCG CAGCACGCCT GAGCCCACAG GGAAGATCAT AGAGAAAGAG 
TTGAAGCCCA GAAACATGAA GGCGGACATG ACTGAACCGC TGTCCCAGTA CGACCTCAAC 
ACTTACTCGA TTGAGAGTGA CTCTGGAGCG TCGGTTCCCA GTCCAGAGGT TTTCCAGGAT 
TCTCTGTCTA AAAAGCAGCG CCGTCCGCAG GAGGACGAGC GCAGGAGGAA GGAGCAGGGC 
CCTCCGAGTT CAGTAAAGAG CAGAACAATT GCTTCAAAGA TGCCTCCGCG GTTTGCCAAG 
AAGCAGGGCA GCATGTCCAT GGAGCAGCCG GAGGAGACGC TCACCGCAAA CAACCTGGGA 
ACTGAGATCT GGGAGACCAA CAGCACAGCT CTGACTGTGC AGTCGTCAGG AGGCGATTCC 
TGGACTAAAC AGGTTTCCTA CACGGGGAGC GAACCCAACT CAGAGGATTC AGATGCGGGA 
CCGGAGCAGA GTAAAGAGCA CAAGCCTGGT CCGATCGGGA ATGAGCGCTC GCTGAAGAAC 
CGGAAAGGCT CAGAGGGTCT GGAGCGCCTG GAGGGATCCA TCACTCCCGT TAACGGCGTG 
GATATTCACG TGGAAAACGT CATTCCTGTG CCGCCCATCG AGTTCGGCGT CAATGCGAAA 
GATTCAGACT TCAGCCTTCC TGCAGGATCT GCAGCCGTGC CCGTCTCCAA CCCCGTCTCC 
AAACTGCAGG ACGCCCTCGC CGGGAACGCG GGCCTGACTC AAGCCATCCC GATGCTGCGC 
AGAGATCACC TGCAGCCCGC CATTACCCTG AACCCCATCA GCTTCCCCTC CGCTGACCTC 
ACGCTCAAGA TGGAGTCCGC CAGGAAGGCG TGGGAGAACT CTCAGGCTGT GCCGGAGCAG 
GGGTCTCCAG GCTCCGGCTC TTCAGCTCAG CCGGTCTGCA GTGTGGGCTC CTCCAGCGTC 
AGCTACAGCT CCTTCTCCAT GCCACCCATG CCTGTGGCGT CTGTGGCCCC GTCCGTCTCC 
ATGCAGGGCA GCCACATCCC TCCTCTGTAT CTGGATGGTC ATGTGTTTCC CAGTCAGCCT 
CGTCTGGTGC CGCCGCTCAC CCAGCAGCCC AGCTACCAGC AGGCGACCCC TCAGCAGATC 
CCCATCTCTC TGCACACGTC TCTGCAGGCT CAGGCTCAGC TGGGTCTGCG TGGAGCTCTG 
CCCGTCTCTC AGTCACAAGA GATCTTCAGC TCCATCCCTC CATTCAGGTC TCAGGTGTAC 
ATGCACCCTA ACCTGTCCCA GGCGAGCCCC ATGGTGCTGT CGGGTGGAGC TGCTCTGAAA 
GGGCCGTACT CCGCCTTCCC CGGCCTGCAG CCCTCAGACC TGGTCAAATC TCAGTCCGGA 
TCCCACTACC AGCCCATCAA CGGCAGCACG CCACTGGTGT ACGACCAGCC CACAGGCATG 
AGCACCTCAC AGCTGATGGA CTCGCAGCTC ATTCAGGTGA CGATGCCGAT GCCAGGCTCT 
CAGCTGCGCT ATGGTTCAGC CCAGCAGCAC CTCATCCTCC CGCAGTCCAT CCAGCTCCAG 
CAGAACCAGA ATCTGTCTGT GGGAGCTCCA CGCCGCATGA TGCCCCCAGG GTCACAGCCA 
GTGCTAAGCG GCAGCAGAGA GGTTTCACAG ATGGACATGA AAGGCTTTCA GTTCTCAGAT 
AAACCCAGCC ACTCTCCTGG AATACCCAGC GGCTCATACA GGCCCGGCTC TGCCAGCCCC 
AGTGGGAAAG CGTCGGGTGC TCCAGCGGTG GCGTCTCTTG CAGGACACTA CACACAGCAG 
GTGTCGGGTC CTCAGGGCAG TATGGTGATG CACATGCGTC CCCCCAGCAG CGGGCCCTTC 
CCCACCCCCA TCCAGCGGCC CATCATGCAG GTCAACAAGC CCGTGATCAT CCGCTCCCCG 
CCGTACCCCG GCCCAAACAC CGGCCCCGCA CACACACACG CACACACACA CAACCCTGAG 
GGCCCCGGCA AGGGCCCGGA GGACGGCCTG AAGGAGTGTG TGGCGTCAGC AGCACCAGTG 
AAAGCGTCAG CGTCTCGCAG CGGAGCCATC AAACCACAGC CGCTCAAACT GGAGGAGGGA 
AAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172102.1
CDS	248..6616
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKECVASAAPVKASASRSGAIKPQPLKLEEGKA

Target ORF information:

RefSeq Version	XM_005172045.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172045.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGT GTATAAGCTG 
GCGTCGTGGC AGCAGTCGGC TCACCCAGCT CAGCCCAGCT CTGCTCACCC GCAGCGGGGC 
TTCTACCCTC CTCATGTGCT GGGCTTCGAC CCACGCTGGA TGATGATGCC ACCATACATG 
GACCCCCGGC TGGCACAGGG CTGCTCTCCT GTGGACTACT ACCCTCCTGG AGTCCACTCA 
TCAGGTTTGG TGAAGCCTGT AATTCAGCCG GATCACCTGA ACAGTCCTGG CTCCACCTCG 
GATGAAGGCT GCCACCCCAG CATGCATCAG GAGAGGAGAG CGCCGTCCAC CGAGCCCTAT 
CAAGTGTGGA ACCAGGACAG CTACCCCTCA GCGCGCAGTT TCACCCCGCC GTATCAGCGG 
ACGCATGAGA ACGGGGAGAG AGCTCCAGCG GACGACAGGA GCGACAGATC CACATCCAGA 
CACGACTCTT ATGAAGAGCA CAGACACGAG CGCACGGACA GCCCAGCCGA GGAAACAACA 
CACCAGGGTT TCCGGCAGGG CAGAGTGTCA GACGGGTCCC AGCGAGAACC CATGATGCTC 
AGCAGGACTC ATGATGATGC TCAACACCAG AAAGAGGAGC CCTACGACAG CAAGGAGAAA 
AGCTATGAGG CTGATTTCTG GAGGCGAGAC GTGCGGAAGG AGAGCAGCGG TCAGACGCAG 
TGGTCCGATC ACGGCTCCAG CAGCAGCAGC AGCATCAGTC AGCCCTCCGA GACCAGCGGC 
AGAACCCTGC GCAGAACCGG ACCCATCAAA AAACCTGTGC TCAAGCCTCT GAAGGTGGAG 
GATAAAGAGA ATGAGAAGCC CAAAGCGGAG CCTGAGGAGA AGCCGGTCCC CTACAGGCTG 
GAGAAGGAGG TGATCACCAA TGTGTACGAC CTGAAGAAAG ACGCTCCGCA CGTTTCAAAC 
AGACACTCCA GCAGCGTCGA AGACAAACAA GCAGACGCTC TGAAGATGGA GAAAACCAGC 
GCGTTATCTG AAGAGCTCCA CAAGGAGAGC TGCTGGGAGA AATCCCAATC CGACTGCTCC 
GACAGCAGAG ATCCGACCGC TCCGAGGCGC AACAACTGGA TCTTCATTGA TGAGGAGCAG 
GCGTTCGCTG GAGCACGCGG TCGAGGACGG GGACGTGGCT TTCGGGAGTT TAATTCTCGC 
GGAGGCGGAG CTCGTGGAGG ACGCAGCGAC ACTAATAGAG GAGCGTACAA TAATAACAAC 
AACAACACGG GCGCTCAGAG ACTCACACGA GGACGCGGAA ACCGAGTGTT TAGAGGGGAC 
GATCTGCAGA GGGGAAAACC GCGCAGACGA AACGTCAGCG AAACGCACAG CGAAACCTCC 
GAGTACGAAG AGCAGCCCAA ACGTCCTCGG CAGAAGGTGT CGGAGAACGG CGGAGAAGCG 
TCAGGAGAGG TTAAAAAAGC CGACAGGGAA TCCTGGAGAT CCAATAAAGT CTACACTGAC 
GATCAAAGCG AGAAACCCAA ATCTAGAGTG TTCGGGCGTT CGTTCCCGCC GCGCCTCAAT 
GCCGCGTATA ATCGCGGATT CACCGGAACC AGAGATATTT CAACGTGGAG AGGAAGAGGA 
ACGCAATTCG GGAGTTCCTC CAGTCAGGAA AACGGCTACA ATTTCGTTGC GGACTCTTAT 
TCGAAGCGCA CCGAGCCGCT GAAGTATCCT GCTAAATTCA CGGGCACGTT TGCGGAGAAC 
GGCGTGGAGG ATCGTGACGG AGGATATTAT GTTGATGATA ATCCGGATAA TCGTCCGCTG 
AGGAGAAGGC GTCCACCACG GCAGGATAAA CCTCCACGGT TCCGCCGTCT GCGTCAGGAG 
CGGGACGGAG CGGGAGCCTG GAGTAATGAT GACTACGTGA ACGGAGAGTT TCCCAATCAG 
TGGCCGAGCA GAGCGAAGAG TGCGGAGGAG CACTGGCAGA ATCACTACCC TGGAGGACGC 
TCGCAGTCAC AGGAGATGAC GGCTCAGAGT CAGGGTGAGG ACTGGGAAAC GGGTTCAGAC 
AACAGTGATT TCGGTGACTG GCGGGAGAAG CGAGGGCCGC ACGGGGATCC GCTGACAGAC 
GCGGCTCTCG GAGATCCGGG ATCTGAGAAG AGAGAACTCT CCAAACGCAG CTTTTCCAGC 
CAGCGGCCGC TGGACCGACA GAACCGCAAG AATGACGTGT CTGTGATGGA GAACAGCAAG 
ATGGGCCGTC CGGCAGATGC AGGATCCAGA AACGACAGCT GGCAGAATGG AGTCGCGTCC 
GGCAGCAAAC GATCTCCAGA AGAGTCTGTC AGCGGTCTGA GCTCTGCGTC GGTGTACGGT 
GTGGAGCAGA ACGAAGACCG CAGCACGCCT GAGCCCACAG GGAAGATCAT AGAGAAAGAG 
TTGAAGCCCA GAAACATGAA GGCGGACATG ACTGAACCGC TGTCCCAGTA CGACCTCAAC 
ACTTACTCGA TTGAGAGTGA CTCTGGAGCG TCGGTTCCCA GTCCAGAGGT TTTCCAGGAT 
TCTCTGTCTA AAAAGCAGCG CCGTCCGCAG GAGGACGAGC GCAGGAGGAA GGAGCAGGGC 
CCTCCGAGTT CAGTAAAGAG CAGAACAATT GCTTCAAAGA TGCCTCCGCG GTTTGCCAAG 
AAGCAGGGCA GCATGTCCAT GGAGCAGCCG GAGGAGACGC TCACCGCAAA CAACCTGGGA 
ACTGAGATCT GGGAGACCAA CAGCACAGCT CTGACTGTGC AGTCGTCAGG AGGCGATTCC 
TGGACTAAAC AGGTTTCCTA CACGGGGAGC GAACCCAACT CAGAGGATTC AGATGCGGGA 
CCGGAGCAGA GTAAAGAGCA CAAGCCTGGT CCGATCGGGA ATGAGCGCTC GCTGAAGAAC 
CGGAAAGGCT CAGAGGGTCT GGAGCGCCTG GAGGGATCCA TCACTCCCGT TAACGGCGTG 
GATATTCACG TGGAAAACGT CATTCCTGTG CCGCCCATCG AGTTCGGCGT CAATGCGAAA 
GATTCAGACT TCAGCCTTCC TGCAGGATCT GCAGCCGTGC CCGTCTCCAA CCCCGTCTCC 
AAACTGCAGG ACGCCCTCGC CGGGAACGCG GGCCTGACTC AAGCCATCCC GATGCTGCGC 
AGAGATCACC TGCAGCCCGC CATTACCCTG AACCCCATCA GCTTCCCCTC CGCTGACCTC 
ACGCTCAAGA TGGAGTCCGC CAGGAAGGCG TGGGAGAACT CTCAGGCTGT GCCGGAGCAG 
GGGTCTCCAG GCTCCGGCTC TTCAGCTCAG CCGGTCTGCA GTGTGGGCTC CTCCAGCGTC 
AGCTACAGCT CCTTCTCCAT GCCACCCATG CCTGTGGCGT CTGTGGCCCC GTCCGTCTCC 
ATGCAGGGCA GCCACATCCC TCCTCTGTAT CTGGATGGTC ATGTGTTTCC CAGTCAGCCT 
CGTCTGGTGC CGCCGCTCAC CCAGCAGCCC AGCTACCAGC AGGCGACCCC TCAGCAGATC 
CCCATCTCTC TGCACACGTC TCTGCAGGCT CAGGCTCAGC TGGGTCTGCG TGGAGCTCTG 
CCCGTCTCTC AGTCACAAGA GATCTTCAGC TCCATCCCTC CATTCAGGTC TCAGGTGTAC 
ATGCACCCTA ACCTGTCCCA GGCGAGCCCC ATGGTGCTGT CGGGTGGAGC TGCTCTGAAA 
GGGCCGTACT CCGCCTTCCC CGGCCTGCAG CCCTCAGACC TGGTCAAATC TCAGTCCGGA 
TCCCACTACC AGCCCATCAA CGGCAGCACG CCACTGGTGT ACGACCAGCC CACAGGCATG 
AGCACCTCAC AGCTGATGGA CTCGCAGCTC ATTCAGGTGA CGATGCCGAT GCCAGGCTCT 
CAGCTGCGCT ATGGTTCAGC CCAGCAGCAC CTCATCCTCC CGCAGTCCAT CCAGCTCCAG 
CAGAACCAGA ATCTGTCTGT GGGAGCTCCA CGCCGCATGA TGCCCCCAGG GTCACAGCCA 
GTGCTAAGCG GCAGCAGAGA GGTTTCACAG ATGGACATGA AAGGCTTTCA GTTCTCAGAT 
AAACCCAGCC ACTCTCCTGG AATACCCAGC GGCTCATACA GGCCCGGCTC TGCCAGCCCC 
AGTGGGAAAG CGTCGGGTGC TCCAGCGGTG GCGTCTCTTG CAGGACACTA CACACAGCAG 
GTGTCGGGTC CTCAGGGCAG TATGGTGATG CACATGCGTC CCCCCAGCAG CGGGCCCTTC 
CCCACCCCCA TCCAGCGGCC CATCATGCAG GTCAACAAGC CCGTGATCAT CCGCTCCCCG 
CCGTACCCCG GCCCAAACAC CGGCCCCGCA CACACACACG CACACACACA CAACCCTGAG 
GGCCCCGGCA AGGGCCCGGA GGACGGCCTG AAGGAGTGTG TGGCGTCAGC AGCACCAGTG 
AAAGCGTCAG CGTCTCGCAG CGGAGCCATC AAACCACAGC CGCTCAAACT GGAGGAGGGA 
AAAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172046.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172103.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	ODa11314
Clone ID Related Accession (Same CDS sequence)	XM_005172046.4 , XM_005172046.3
Accession Version	XM_005172046.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172046.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG TGAGCCACCC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172103.1
CDS	244..6540
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKVSHPL

Target ORF information:

RefSeq Version	XM_005172046.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172046.4

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG TGAGCCACCC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRRC2B ( XM_005172046.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005172103.1 Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

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CloneID	ODa11314
Clone ID Related Accession (Same CDS sequence)	XM_005172046.4 , XM_005172046.3
Accession Version	XM_005172046.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein PRRC2B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG TGAGCCACCC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172103.1
CDS	248..6544
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKTTVVARHGLQSLGKVAAARRMPPPAHLPSLKSESKGNDPNVIIVPKDGTGWANKQDQPDPKSSVASSAQLPEPQPPLALQKSVSNLQKPMPITSPESTSTGGPKQWAQLNGKAVDQDGLKVSSRLQPFSHEDFPTLKAAGEQDKVGKERSVFDPSYGPGPSLRPQNVSSWREGGGRNLQPPSLSSAPPGDTDTKSSGSVEAPLPPPPSTSTSTSSSASPAASSSSTAAAAAPSSEVKEPSLRPAPPLTRRAAPPALQYQHHTTTTYHDMLPAFMCPKETREAPGSSDGPAAVVAPVRFDSRPIFRPPFPAPEPVNGDVRREPRFPRAPPRPSSRPIRRPGDRAPRPAIINPDDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEDHAPREKSKIWDDWDHHHHHHHLQGQHSSQSSGDGAFPQEPEDEPYLRQQEPPASRKTSTRFPAEPQQKASVNSESAEEQDEPQRSAPPRGKFVSADVSAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTEKPARSGEADGKELAQSPARRSAKPQQDGWQYGSKEVTDTPAESASSQDYKDEGCNFHNNDDEDDDGGESTSPVPEYSRHQKPVPPRFQKQQQQQEQVYKLASWQQSAHPAQPSSAHPQRGFYPPHVLGFDPRWMMMPPYMDPRLAQGCSPVDYYPPGVHSSGLVKPVIQPDHLNSPGSTSDEGCHPSMHQERRAPSTEPYQVWNQDSYPSARSFTPPYQRTHENGERAPADDRSDRSTSRHDSYEEHRHERTDSPAEETTHQGFRQGRVSDGSQREPMMLSRTHDDAQHQKEEPYDSKEKSYEADFWRRDVRKESSGQTQWSDHGSSSSSSISQPSETSGRTLRRTGPIKKPVLKPLKVEDKENEKPKAEPEEKPVPYRLEKEVITNVYDLKKDAPHVSNRHSSSVEDKQADALKMEKTSALSEELHKESCWEKSQSDCSDSRDPTAPRRNNWIFIDEEQAFAGARGRGRGRGFREFNSRGGGARGGRSDTNRGAYNNNNNNTGAQRLTRGRGNRVFRGDDLQRGKPRRRNVSETHSETSEYEEQPKRPRQKVSENGGEASGEVKKADRESWRSNKVYTDDQSEKPKSRVFGRSFPPRLNAAYNRGFTGTRDISTWRGRGTQFGSSSSQENGYNFVADSYSKRTEPLKYPAKFTGTFAENGVEDRDGGYYVDDNPDNRPLRRRRPPRQDKPPRFRRLRQERDGAGAWSNDDYVNGEFPNQWPSRAKSAEEHWQNHYPGGRSQSQEMTAQSQGEDWETGSDNSDFGDWREKRGPHGDPLTDAALGDPGSEKRELSKRSFSSQRPLDRQNRKNDVSVMENSKMGRPADAGSRNDSWQNGVASGSKRSPEESVSGLSSASVYGVEQNEDRSTPEPTGKIIEKELKPRNMKADMTEPLSQYDLNTYSIESDSGASVPSPEVFQDSLSKKQRRPQEDERRRKEQGPPSSVKSRTIASKMPPRFAKKQGSMSMEQPEETLTANNLGTEIWETNSTALTVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQSKEHKPGPIGNERSLKNRKGSEGLERLEGSITPVNGVDIHVENVIPVPPIEFGVNAKDSDFSLPAGSAAVPVSNPVSKLQDALAGNAGLTQAIPMLRRDHLQPAITLNPISFPSADLTLKMESARKAWENSQAVPEQGSPGSGSSAQPVCSVGSSSVSYSSFSMPPMPVASVAPSVSMQGSHIPPLYLDGHVFPSQPRLVPPLTQQPSYQQATPQQIPISLHTSLQAQAQLGLRGALPVSQSQEIFSSIPPFRSQVYMHPNLSQASPMVLSGGAALKGPYSAFPGLQPSDLVKSQSGSHYQPINGSTPLVYDQPTGMSTSQLMDSQLIQVTMPMPGSQLRYGSAQQHLILPQSIQLQQNQNLSVGAPRRMMPPGSQPVLSGSREVSQMDMKGFQFSDKPSHSPGIPSGSYRPGSASPSGKASGAPAVASLAGHYTQQVSGPQGSMVMHMRPPSSGPFPTPIQRPIMQVNKPVIIRSPPYPGPNTGPAHTHAHTHNPEGPGKGPEDGLKVSHPL

Target ORF information:

RefSeq Version	XM_005172046.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio proline-rich coiled-coil 2B (prrc2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172046.3

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATTACCAAG TCCAAGGATG GGAAAAGCAA GTATTCATCA 
CTCAGCCTAT TTGACAAGTA CAAGGGGAAG TCAATAGAAA CTCAGAAAAC CACAGTTGTT 
GCACGTCATG GCTTACAGAG TCTTGGTAAA GTGGCCGCAG CCCGGCGAAT GCCTCCTCCC 
GCTCACCTGC CGAGTCTGAA GTCTGAGAGT AAAGGAAACG ATCCCAACGT GATTATCGTG 
CCCAAAGACG GTACAGGATG GGCAAACAAG CAGGATCAAC CCGATCCAAA GAGTTCCGTT 
GCATCATCAG CACAGCTGCC GGAGCCGCAG CCGCCGCTGG CTTTACAGAA ATCTGTGTCC 
AATCTTCAGA AGCCCATGCC AATAACCAGC CCGGAGAGCA CAAGTACAGG TGGACCAAAG 
CAATGGGCAC AGCTCAATGG AAAGGCAGTC GATCAAGATG GTTTAAAGGT CTCAAGCCGA 
CTGCAGCCCT TCTCTCACGA GGATTTTCCG ACGCTGAAGG CAGCAGGAGA ACAGGACAAA 
GTTGGCAAGG AAAGAAGCGT CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCG 
CAGAACGTGT CGAGCTGGAG GGAGGGTGGA GGGAGGAATC TGCAGCCTCC ATCTCTGTCC 
TCTGCTCCTC CGGGCGACAC CGACACCAAG AGCAGCGGCT CAGTGGAGGC TCCACTTCCT 
CCTCCACCCT CCACTTCCAC CTCCACTTCC TCCTCCGCTT CCCCCGCCGC TTCCTCTTCC 
TCCACTGCCG CCGCCGCTGC TCCGTCCTCT GAAGTGAAGG AGCCATCGCT GCGTCCCGCT 
CCACCCCTGA CCCGCAGAGC TGCTCCACCT GCTCTGCAGT ACCAGCACCA CACCACCACC 
ACCTACCACG ACATGCTGCC CGCCTTCATG TGTCCTAAAG AGACGCGTGA GGCCCCGGGC 
TCCTCTGACG GCCCTGCCGC TGTCGTGGCT CCTGTACGAT TCGACTCCAG ACCGATCTTC 
AGACCGCCGT TTCCTGCGCC GGAGCCCGTC AACGGTGATG TACGAAGAGA GCCCCGTTTC 
CCCAGAGCCC CCCCACGACC CTCATCTCGC CCCATCCGGC GGCCTGGTGA CAGGGCGCCT 
CGTCCTGCCA TCATCAACCC CGACGACCTG AAGGACCTGG ATGAGCTGGA TAATGACTGT 
GAGGACGGCT GGGCAGGTCT CCATGAGGAA GTGGATTACA GTGAGAAACT GAAGTTCAGT 
GATGATGAGG AAGATCACGC CCCGCGGGAG AAGAGCAAGA TCTGGGACGA CTGGGATCAT 
CATCATCACC ATCACCATCT TCAGGGCCAG CATTCCTCTC AGAGTTCAGG AGACGGAGCG 
TTCCCCCAGG AGCCCGAAGA CGAGCCGTAT TTACGCCAGC AGGAGCCTCC GGCCTCCAGG 
AAGACCAGCA CACGCTTCCC TGCAGAACCA CAGCAGAAGG CCAGTGTGAA CAGTGAGTCT 
GCAGAGGAGC AGGATGAGCC CCAGCGGTCG GCTCCTCCTC GAGGGAAGTT CGTCTCAGCA 
GACGTCTCGG CGGTGGAGCG AGCCCGTCGG CGCCGCGAGG AGGAGGAGCG CAGGGCTCGA 
GAGGAGAGAC TGGCGGCCTG CGCTGAGAAA CTCAAGAAAC TGGATGAGAA GTTTGGCAAA 
ACGGAGAAAC CTGCTCGCTC TGGAGAAGCA GACGGCAAGG AGCTGGCGCA GTCTCCCGCT 
CGCAGATCCG CAAAACCCCA GCAGGATGGC TGGCAGTACG GCTCCAAAGA AGTGACGGAC 
ACGCCAGCGG AGTCAGCCTC CAGTCAGGAT TATAAAGATG AAGGCTGTAA CTTCCACAAT 
AATGATGATG AGGATGATGA TGGTGGCGAG TCCACGTCTC CTGTGCCGGA GTACAGCCGA 
CATCAGAAGC CTGTGCCGCC ACGCTTCCAG AAGCAGCAGC AGCAGCAGGA GCAGGTGTAT 
AAGCTGGCGT CGTGGCAGCA GTCGGCTCAC CCAGCTCAGC CCAGCTCTGC TCACCCGCAG 
CGGGGCTTCT ACCCTCCTCA TGTGCTGGGC TTCGACCCAC GCTGGATGAT GATGCCACCA 
TACATGGACC CCCGGCTGGC ACAGGGCTGC TCTCCTGTGG ACTACTACCC TCCTGGAGTC 
CACTCATCAG GTTTGGTGAA GCCTGTAATT CAGCCGGATC ACCTGAACAG TCCTGGCTCC 
ACCTCGGATG AAGGCTGCCA CCCCAGCATG CATCAGGAGA GGAGAGCGCC GTCCACCGAG 
CCCTATCAAG TGTGGAACCA GGACAGCTAC CCCTCAGCGC GCAGTTTCAC CCCGCCGTAT 
CAGCGGACGC ATGAGAACGG GGAGAGAGCT CCAGCGGACG ACAGGAGCGA CAGATCCACA 
TCCAGACACG ACTCTTATGA AGAGCACAGA CACGAGCGCA CGGACAGCCC AGCCGAGGAA 
ACAACACACC AGGGTTTCCG GCAGGGCAGA GTGTCAGACG GGTCCCAGCG AGAACCCATG 
ATGCTCAGCA GGACTCATGA TGATGCTCAA CACCAGAAAG AGGAGCCCTA CGACAGCAAG 
GAGAAAAGCT ATGAGGCTGA TTTCTGGAGG CGAGACGTGC GGAAGGAGAG CAGCGGTCAG 
ACGCAGTGGT CCGATCACGG CTCCAGCAGC AGCAGCAGCA TCAGTCAGCC CTCCGAGACC 
AGCGGCAGAA CCCTGCGCAG AACCGGACCC ATCAAAAAAC CTGTGCTCAA GCCTCTGAAG 
GTGGAGGATA AAGAGAATGA GAAGCCCAAA GCGGAGCCTG AGGAGAAGCC GGTCCCCTAC 
AGGCTGGAGA AGGAGGTGAT CACCAATGTG TACGACCTGA AGAAAGACGC TCCGCACGTT 
TCAAACAGAC ACTCCAGCAG CGTCGAAGAC AAACAAGCAG ACGCTCTGAA GATGGAGAAA 
ACCAGCGCGT TATCTGAAGA GCTCCACAAG GAGAGCTGCT GGGAGAAATC CCAATCCGAC 
TGCTCCGACA GCAGAGATCC GACCGCTCCG AGGCGCAACA ACTGGATCTT CATTGATGAG 
GAGCAGGCGT TCGCTGGAGC ACGCGGTCGA GGACGGGGAC GTGGCTTTCG GGAGTTTAAT 
TCTCGCGGAG GCGGAGCTCG TGGAGGACGC AGCGACACTA ATAGAGGAGC GTACAATAAT 
AACAACAACA ACACGGGCGC TCAGAGACTC ACACGAGGAC GCGGAAACCG AGTGTTTAGA 
GGGGACGATC TGCAGAGGGG AAAACCGCGC AGACGAAACG TCAGCGAAAC GCACAGCGAA 
ACCTCCGAGT ACGAAGAGCA GCCCAAACGT CCTCGGCAGA AGGTGTCGGA GAACGGCGGA 
GAAGCGTCAG GAGAGGTTAA AAAAGCCGAC AGGGAATCCT GGAGATCCAA TAAAGTCTAC 
ACTGACGATC AAAGCGAGAA ACCCAAATCT AGAGTGTTCG GGCGTTCGTT CCCGCCGCGC 
CTCAATGCCG CGTATAATCG CGGATTCACC GGAACCAGAG ATATTTCAAC GTGGAGAGGA 
AGAGGAACGC AATTCGGGAG TTCCTCCAGT CAGGAAAACG GCTACAATTT CGTTGCGGAC 
TCTTATTCGA AGCGCACCGA GCCGCTGAAG TATCCTGCTA AATTCACGGG CACGTTTGCG 
GAGAACGGCG TGGAGGATCG TGACGGAGGA TATTATGTTG ATGATAATCC GGATAATCGT 
CCGCTGAGGA GAAGGCGTCC ACCACGGCAG GATAAACCTC CACGGTTCCG CCGTCTGCGT 
CAGGAGCGGG ACGGAGCGGG AGCCTGGAGT AATGATGACT ACGTGAACGG AGAGTTTCCC 
AATCAGTGGC CGAGCAGAGC GAAGAGTGCG GAGGAGCACT GGCAGAATCA CTACCCTGGA 
GGACGCTCGC AGTCACAGGA GATGACGGCT CAGAGTCAGG GTGAGGACTG GGAAACGGGT 
TCAGACAACA GTGATTTCGG TGACTGGCGG GAGAAGCGAG GGCCGCACGG GGATCCGCTG 
ACAGACGCGG CTCTCGGAGA TCCGGGATCT GAGAAGAGAG AACTCTCCAA ACGCAGCTTT 
TCCAGCCAGC GGCCGCTGGA CCGACAGAAC CGCAAGAATG ACGTGTCTGT GATGGAGAAC 
AGCAAGATGG GCCGTCCGGC AGATGCAGGA TCCAGAAACG ACAGCTGGCA GAATGGAGTC 
GCGTCCGGCA GCAAACGATC TCCAGAAGAG TCTGTCAGCG GTCTGAGCTC TGCGTCGGTG 
TACGGTGTGG AGCAGAACGA AGACCGCAGC ACGCCTGAGC CCACAGGGAA GATCATAGAG 
AAAGAGTTGA AGCCCAGAAA CATGAAGGCG GACATGACTG AACCGCTGTC CCAGTACGAC 
CTCAACACTT ACTCGATTGA GAGTGACTCT GGAGCGTCGG TTCCCAGTCC AGAGGTTTTC 
CAGGATTCTC TGTCTAAAAA GCAGCGCCGT CCGCAGGAGG ACGAGCGCAG GAGGAAGGAG 
CAGGGCCCTC CGAGTTCAGT AAAGAGCAGA ACAATTGCTT CAAAGATGCC TCCGCGGTTT 
GCCAAGAAGC AGGGCAGCAT GTCCATGGAG CAGCCGGAGG AGACGCTCAC CGCAAACAAC 
CTGGGAACTG AGATCTGGGA GACCAACAGC ACAGCTCTGA CTGTGCAGTC GTCAGGAGGC 
GATTCCTGGA CTAAACAGGT TTCCTACACG GGGAGCGAAC CCAACTCAGA GGATTCAGAT 
GCGGGACCGG AGCAGAGTAA AGAGCACAAG CCTGGTCCGA TCGGGAATGA GCGCTCGCTG 
AAGAACCGGA AAGGCTCAGA GGGTCTGGAG CGCCTGGAGG GATCCATCAC TCCCGTTAAC 
GGCGTGGATA TTCACGTGGA AAACGTCATT CCTGTGCCGC CCATCGAGTT CGGCGTCAAT 
GCGAAAGATT CAGACTTCAG CCTTCCTGCA GGATCTGCAG CCGTGCCCGT CTCCAACCCC 
GTCTCCAAAC TGCAGGACGC CCTCGCCGGG AACGCGGGCC TGACTCAAGC CATCCCGATG 
CTGCGCAGAG ATCACCTGCA GCCCGCCATT ACCCTGAACC CCATCAGCTT CCCCTCCGCT 
GACCTCACGC TCAAGATGGA GTCCGCCAGG AAGGCGTGGG AGAACTCTCA GGCTGTGCCG 
GAGCAGGGGT CTCCAGGCTC CGGCTCTTCA GCTCAGCCGG TCTGCAGTGT GGGCTCCTCC 
AGCGTCAGCT ACAGCTCCTT CTCCATGCCA CCCATGCCTG TGGCGTCTGT GGCCCCGTCC 
GTCTCCATGC AGGGCAGCCA CATCCCTCCT CTGTATCTGG ATGGTCATGT GTTTCCCAGT 
CAGCCTCGTC TGGTGCCGCC GCTCACCCAG CAGCCCAGCT ACCAGCAGGC GACCCCTCAG 
CAGATCCCCA TCTCTCTGCA CACGTCTCTG CAGGCTCAGG CTCAGCTGGG TCTGCGTGGA 
GCTCTGCCCG TCTCTCAGTC ACAAGAGATC TTCAGCTCCA TCCCTCCATT CAGGTCTCAG 
GTGTACATGC ACCCTAACCT GTCCCAGGCG AGCCCCATGG TGCTGTCGGG TGGAGCTGCT 
CTGAAAGGGC CGTACTCCGC CTTCCCCGGC CTGCAGCCCT CAGACCTGGT CAAATCTCAG 
TCCGGATCCC ACTACCAGCC CATCAACGGC AGCACGCCAC TGGTGTACGA CCAGCCCACA 
GGCATGAGCA CCTCACAGCT GATGGACTCG CAGCTCATTC AGGTGACGAT GCCGATGCCA 
GGCTCTCAGC TGCGCTATGG TTCAGCCCAG CAGCACCTCA TCCTCCCGCA GTCCATCCAG 
CTCCAGCAGA ACCAGAATCT GTCTGTGGGA GCTCCACGCC GCATGATGCC CCCAGGGTCA 
CAGCCAGTGC TAAGCGGCAG CAGAGAGGTT TCACAGATGG ACATGAAAGG CTTTCAGTTC 
TCAGATAAAC CCAGCCACTC TCCTGGAATA CCCAGCGGCT CATACAGGCC CGGCTCTGCC 
AGCCCCAGTG GGAAAGCGTC GGGTGCTCCA GCGGTGGCGT CTCTTGCAGG ACACTACACA 
CAGCAGGTGT CGGGTCCTCA GGGCAGTATG GTGATGCACA TGCGTCCCCC CAGCAGCGGG 
CCCTTCCCCA CCCCCATCCA GCGGCCCATC ATGCAGGTCA ACAAGCCCGT GATCATCCGC 
TCCCCGCCGT ACCCCGGCCC AAACACCGGC CCCGCACACA CACACGCACA CACACACAAC 
CCTGAGGGCC CCGGCAAGGG CCCGGAGGAC GGCCTGAAGG TGAGCCACCC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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PRRC2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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