nckap5l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nckap5l
Entrez Gene ID	100493761
Full Name	NCK-associated protein 5-like
General protein information	Preferred Names NCK-associated protein 5-like Names nck-associated protein 5-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911844.3	XP_004911901.1	nck-associated protein 5-like
XM_002935119.5	XP_002935165.3	nck-associated protein 5-like
XM_004911845.4	XP_004911902.2	nck-associated protein 5-like
XM_002935119.4	XP_002935165.2	nck-associated protein 5-like
XM_031897145.1	XP_031753005.1	nck-associated protein 5-like
XM_004911845.3	XP_004911902.1	nck-associated protein 5-like
XM_004911844.4	XP_004911901.2	nck-associated protein 5-like

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NCKAP5L	XP_003313756.1
Bos taurus (cattle)	NCKAP5L	NP_001192468.1
	LOC711480	XP_002808025.1
Rattus norvegicus (Norway rat)	Nckap5l	NP_001258298.1
Homo sapiens (human)	NCKAP5L	NP_001032895.2
Canis lupus familiaris (dog)	NCKAP5L	XP_003639998.1
Mus musculus (house mouse)	Nckap5l	NP_001001884.1
Xenopus tropicalis (tropical clawed frog)	nckap5l	XP_004911902.1

Gene Ontology

The following nckap5l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nckap5l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52646	XM_002935119.5 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52646	XM_004911845.4 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52646	XM_004911844.4 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52646	XM_031897145.1 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa05967	XM_004911845.3 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa05967	XM_002935119.4 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa05967	XM_004911844.3 Latest version!	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nckap5l ( XM_002935119.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935165.3 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52646
Clone ID Related Accession (Same CDS sequence)	XM_002935119.5 , XM_004911845.4 , XM_031897145.1 , XM_004911844.4
Accession Version	XM_002935119.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935119.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935165.3
CDS	170..3961
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPAASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_002935119.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935119.5

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_004911845.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911902.2 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa52646
Clone ID Related Accession (Same CDS sequence)	XM_002935119.5 , XM_004911845.4 , XM_031897145.1 , XM_004911844.4
Accession Version	XM_004911845.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911845.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911902.2
CDS	770..4561
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPAASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_004911845.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911845.4

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_031897145.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753005.1 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X4, mRNA.

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CloneID	OXa52646
Clone ID Related Accession (Same CDS sequence)	XM_002935119.5 , XM_004911845.4 , XM_031897145.1 , XM_004911844.4
Accession Version	XM_031897145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753005.1
CDS	154..3945
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPAASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_031897145.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897145.1

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_004911844.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911901.2 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52646
Clone ID Related Accession (Same CDS sequence)	XM_002935119.5 , XM_004911845.4 , XM_031897145.1 , XM_004911844.4
Accession Version	XM_004911844.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911844.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911901.2
CDS	677..4468
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPAASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_004911844.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911844.4

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGCA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_004911844.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911901.1 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05967
Clone ID Related Accession (Same CDS sequence)	XM_004911844.3 , XM_002935119.4 , XM_004911845.3
Accession Version	XM_004911844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911901.1
CDS	752..4543
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPVASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_004911844.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911844.3

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_002935119.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935165.2 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

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CloneID	OXa05967
Clone ID Related Accession (Same CDS sequence)	XM_004911844.3 , XM_002935119.4 , XM_004911845.3
Accession Version	XM_002935119.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935119.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935165.2
CDS	169..3960
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPVASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_002935119.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935119.4

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap5l ( XM_004911845.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911902.1 Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

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CloneID	OXa05967
Clone ID Related Accession (Same CDS sequence)	XM_004911844.3 , XM_002935119.4 , XM_004911845.3
Accession Version	XM_004911845.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911845.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911902.1
CDS	846..4637
Translation	MTSEVEDEIRLLTGGVSEPLVIQDLLERLRELEAENTALAQANETQRETYERCLDEVANHVVQALLNQKDLREECIKLKKRVFDLERQNRTLNDLFHQKLQLSCGSSPELHPLQALSDSQPGELERLSGSLPLGQCAAQREENQRSSRAQSACRSLDAMSPFLRKKAQILEVLRRLEGTGSQLGSSCTAPPVLLGGASPGGNGLRLGSDPGLDYLNGEGLTPPELGGDEPWASCLLLAQNGWEELLKWKPVQREPPAGSPGEGGGERLGLAAGDDTQQLARQAEGSSSSSDDEIGDPPPLPSELRQRLPLTDLAKGLGPCLCSHGDKRMPVEGHSDGTGLSRGHIGHLHSLTSYSRSLRDMVESPQLSGTQQMVTERGDSLSWSPGKQAILDGLVDTCPPSPGSTNVQRDTSVLNLKDTSLDLPDDDMQSSSGSSNPTSGDPQDTLISSSEPRTPHIPSPAKFLKFLKLPGSGEKLQSPNPLRLSPQLTQSSKIPCRSNNYESFPSPRLGRKGIEDIPDPSTEHEPTFPSDLPLSEPNRQGQCERTSNSIINRPEVQCCTKKSHDYENIPPKTSELPTTNQINESLEVSSHLSVGVCTTNCTPGLCIHTQGNCNEETKGHVSSPFIRKPVASRKPPETPGHLPFRERMGKLRGAENPQGDTAATGSESSSMVADGPDTRPSMKRSVGVSSLRTPEPGSTESYPPRYHGQKAESARTRQSGTHCPISSPHGIRTPAKAPPVPTKSPRSPHGSPTKLPAKSPSKATGKPLISRPLSEEVRPGSRQATHASEEKQKAQSAGASKKNAICPEYACPRNASPQGTESLPLSALHSAIEEKVMKGIKENMLRLQEQHRTPPPDPKQRNSSGIASWFGLKKSKLPALSRRPEPGRLKEDKRERTTGVLPRSKEVKGESLNISMLMEKAEDLRKALQEERAYINGLSIEKTRSAASVESTQNPRPLTADNFMQQLLNRVDGKDPPNDGRVEGKAPLRDFPRLSPESKEARPFRPQRNGIVTHVQRSEQKSLEQNREVVLPPDGRIPEPNPSQHFAACDSLTRTLDSGIGTFPPPDYCGGTPTKGTPKLKPRLESPSVLPVGRFSAFPKVPRRARTLERDIRGVEEIFSSGQHQSVPAFHALLAEPEPPMGHRVYVEDSSREHSRPQQGKNWTFPNAKVSTNSTDGFRSRNHDLEEIPSEGDRDCDVSGYAHPSLCFPGSVSSRTPSTSEVGEEGTSETKSRDNEQCQTGLENSESLSDSLYDSLSSCGSQG

Target ORF information:

RefSeq Version	XM_004911845.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 5-like (nckap5l), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911845.3

ORF Insert Sequence:

ATGACTTCGG AGGTGGAGGA TGAAATTCGG CTTCTGACTG GAGGAGTATC TGAGCCACTT 
GTCATTCAGG ATTTGTTGGA GCGATTGCGG GAACTAGAGG CAGAAAACAC TGCACTTGCT 
CAAGCCAATG AAACGCAGCG AGAGACCTAT GAGCGCTGTC TGGATGAGGT TGCAAACCAT 
GTTGTCCAGG CCCTTCTCAA TCAGAAGGAT CTGCGGGAAG AATGCATCAA ACTGAAGAAG 
AGAGTGTTTG ACCTTGAGCG CCAAAACAGA ACCTTAAATG ACCTCTTTCA TCAGAAGTTG 
CAGCTCTCCT GTGGCTCATC TCCCGAGCTC CACCCCCTGC AGGCTCTGTC TGATTCCCAG 
CCTGGTGAAT TGGAGAGGCT CTCTGGCTCA TTACCCTTGG GACAATGTGC TGCACAGAGA 
GAGGAGAACC AGCGCTCCAG CCGAGCCCAG TCAGCTTGCC GCTCTTTGGA TGCAATGTCT 
CCATTCCTCA GAAAAAAGGC TCAGATCTTG GAAGTTCTAA GAAGACTGGA AGGAACTGGA 
TCTCAGTTAG GATCCTCCTG CACTGCCCCA CCAGTTCTGC TTGGTGGTGC ATCACCAGGG 
GGAAATGGTC TGCGTTTAGG ATCAGACCCA GGTCTTGATT ACTTGAATGG TGAAGGACTT 
ACACCTCCAG AACTGGGTGG AGATGAGCCT TGGGCATCAT GCCTGTTGTT AGCTCAGAAT 
GGGTGGGAGG AATTGCTTAA GTGGAAACCA GTTCAACGAG AACCACCTGC AGGTAGCCCA 
GGGGAGGGTG GAGGGGAGAG GCTTGGTTTG GCTGCAGGTG ATGATACACA ACAATTGGCA 
CGACAGGCTG AGGGGAGCTC TTCCTCTTCG GATGATGAGA TAGGTGATCC TCCACCTCTG 
CCAAGTGAGT TAAGGCAACG GCTGCCACTG ACAGACTTGG CTAAAGGCTT GGGGCCTTGC 
CTCTGCTCGC ATGGAGATAA AAGAATGCCT GTTGAGGGCC ACTCTGATGG AACTGGCTTG 
AGTAGGGGAC ACATTGGGCA TCTCCATTCA CTGACATCCT ATAGTCGAAG TTTGAGGGAC 
ATGGTGGAGA GTCCACAGCT GTCAGGGACT CAACAAATGG TTACTGAGAG GGGGGATTCT 
TTGTCATGGT CACCAGGAAA ACAGGCTATA TTGGATGGAC TAGTTGATAC CTGTCCTCCT 
TCTCCTGGGT CTACCAATGT ACAAAGAGAC ACAAGTGTAC TGAATCTAAA GGACACATCC 
CTCGATTTAC CAGATGATGA TATGCAATCA TCATCAGGAA GCTCTAACCC AACCTCTGGG 
GATCCTCAAG ATACACTCAT CTCTTCCTCC GAGCCTCGGA CACCTCACAT TCCATCTCCA 
GCAAAATTTC TCAAGTTCCT TAAACTCCCA GGCTCAGGGG AAAAGTTACA AAGCCCTAAT 
CCCTTGCGCC TCAGTCCACA ACTTACCCAG TCCTCAAAGA TACCTTGTAG AAGCAATAAT 
TATGAATCAT TTCCCTCTCC TCGTTTAGGA AGGAAGGGAA TAGAGGATAT ACCAGATCCC 
AGTACTGAGC ATGAACCAAC TTTCCCCTCT GACTTACCAC TTTCTGAACC AAACAGGCAA 
GGCCAGTGTG AGAGGACTTC AAACAGCATT ATAAACAGGC CAGAAGTGCA GTGTTGCACC 
AAAAAGTCAC ATGATTATGA AAATATCCCA CCTAAAACTT CAGAGCTTCC CACCACAAAC 
CAAATTAATG AATCTTTGGA AGTTTCTTCC CATTTGTCTG TAGGAGTGTG TACTACTAAT 
TGTACCCCAG GACTTTGCAT TCACACTCAG GGTAACTGTA ATGAGGAGAC TAAGGGTCAT 
GTAAGCTCCC CCTTTATTCG AAAACCTGTA GCTTCCAGAA AACCTCCAGA GACACCTGGA 
CATCTCCCTT TCCGAGAAAG GATGGGAAAG TTAAGAGGAG CTGAAAACCC ACAGGGAGAC 
ACAGCTGCAA CAGGATCTGA AAGCAGCAGC ATGGTAGCAG ATGGTCCTGA CACCCGTCCT 
TCTATGAAGC GATCAGTTGG TGTTAGCAGT CTAAGAACAC CTGAACCTGG ATCTACAGAA 
AGTTATCCAC CAAGATACCA TGGCCAAAAA GCAGAGTCTG CACGGACCCG TCAGTCTGGT 
ACTCATTGTC CTATTAGTTC TCCACATGGC ATCCGAACAC CAGCTAAGGC ACCACCTGTC 
CCTACCAAAT CTCCACGTAG CCCTCATGGC AGCCCAACAA AACTACCTGC AAAGTCCCCA 
AGTAAGGCAA CAGGCAAACC TTTGATATCT CGGCCCTTAA GTGAAGAGGT AAGACCAGGC 
TCTCGGCAGG CTACTCATGC AAGTGAAGAA AAACAAAAGG CACAGTCAGC TGGGGCAAGT 
AAGAAAAATG CCATTTGTCC AGAATATGCC TGTCCTCGTA ACGCAAGCCC ACAGGGAACA 
GAAAGCCTTC CGCTTAGTGC TTTACACTCT GCCATTGAAG AGAAAGTGAT GAAAGGCATT 
AAAGAAAACA TGCTGAGGCT GCAGGAGCAA CACCGCACAC CACCACCCGA CCCTAAACAA 
CGCAACTCAA GTGGTATCGC AAGTTGGTTT GGCTTGAAAA AGAGTAAACT GCCAGCACTA 
AGCCGGAGGC CTGAACCTGG CCGCCTGAAA GAGGACAAAA GGGAGAGAAC AACAGGAGTA 
TTGCCTCGCT CAAAGGAAGT TAAAGGGGAA AGCCTAAACA TTTCCATGCT CATGGAGAAG 
GCTGAAGACC TACGTAAGGC TCTGCAGGAG GAGAGAGCTT ATATAAATGG CCTTTCCATT 
GAGAAAACCC GCTCAGCTGC TTCTGTGGAA TCAACGCAGA ATCCACGACC CCTGACCGCT 
GATAACTTCA TGCAGCAGCT GCTGAACCGA GTTGATGGAA AAGATCCACC CAACGATGGC 
AGGGTTGAAG GCAAGGCACC ACTCCGTGAT TTCCCCAGAT TGTCACCTGA GAGTAAAGAG 
GCCAGACCAT TCCGCCCTCA GAGGAATGGA ATTGTGACCC ATGTTCAGCG CTCTGAACAG 
AAAAGCCTTG AGCAGAACAG GGAGGTGGTT CTCCCTCCAG ATGGGAGGAT ACCAGAACCT 
AACCCTTCTC AGCACTTTGC AGCTTGTGAC TCTCTTACTC GAACTCTGGA TAGTGGCATT 
GGCACCTTTC CTCCGCCTGA TTATTGTGGG GGTACTCCAA CCAAAGGCAC CCCAAAATTA 
AAGCCCCGGC TAGAATCGCC CTCTGTTCTC CCCGTGGGAA GGTTTTCTGC TTTCCCTAAA 
GTGCCAAGGA GAGCAAGGAC TTTAGAAAGG GACATCCGGG GCGTGGAGGA AATCTTCTCA 
TCTGGACAGC ACCAGAGCGT CCCAGCCTTT CATGCACTGC TGGCAGAGCC AGAACCACCA 
ATGGGACACA GAGTGTATGT AGAAGATTCT AGCAGGGAGC ACAGCCGGCC ACAGCAGGGC 
AAGAACTGGA CATTTCCCAA TGCAAAGGTC AGCACAAACT CCACAGATGG CTTCCGGTCC 
AGAAACCATG ACCTTGAGGA GATTCCTTCA GAAGGGGACC GAGACTGTGA TGTGTCAGGG 
TACGCACACC CCTCCCTCTG TTTCCCTGGC AGTGTGAGCA GCCGCACTCC AAGTACCTCT 
GAGGTCGGGG AGGAGGGAAC AAGTGAAACC AAATCTCGAG ACAATGAGCA GTGCCAGACT 
GGCCTGGAGA ACTCGGAATC TCTGAGTGAC TCTCTGTATG ATAGCTTATC ATCCTGCGGG 
AGTCAAGGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nckap5l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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