myo5c cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo5c
Entrez Gene ID	100495210
Full Name	myosin VC
General protein information	Preferred Names myosin VC Names unconventional myosin-Vc
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031899192.1	XP_031755052.1	unconventional myosin-Vc isoform X2
XM_012959596.2	XP_012815050.1	unconventional myosin-Vc isoform X1
XM_002936346.4	XP_002936392.2	unconventional myosin-Vc isoform X2
XM_018092428.1	XP_017947917.1	unconventional myosin-Vc isoform X3
XM_002936346.5	XP_002936392.2	unconventional myosin-Vc isoform X3
XM_012959596.3	XP_012815050.1	unconventional myosin-Vc isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYO5C	NP_061198.2
Pan troglodytes (chimpanzee)	MYO5C	XP_510411.3
Schizosaccharomyces pombe (fission yeast)	myo51	NP_596233.1
Arabidopsis thaliana (thale cress)	XID	NP_180882.2
Xenopus tropicalis (tropical clawed frog)	myo5c	XP_002936392.2
Danio rerio (zebrafish)	myo5c	XP_691143.2
	MYO5C	XP_002804829.1
Canis lupus familiaris (dog)	MYO5C	XP_544680.3
Mus musculus (house mouse)	Myo5c	NP_001074791.1
Rattus norvegicus (Norway rat)	Myo5c	XP_006243475.1
Gallus gallus (chicken)	MYO5C	XP_003641891.1
Arabidopsis thaliana (thale cress)	XIA	NP_171954.1

Gene Ontology

The following myo5c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo5c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07362	XM_002936346.5 Latest version!	Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OXa36221	XM_012959596.3 Latest version!	Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OXa43364	XM_018092428.1 Latest version!	Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
OXa55103	XM_031899192.1 Latest version!	Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	Quote Price
View Old Accession Versions >>
OXa07362	XM_002936346.4	Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
OXa36221	XM_012959596.2	Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo5c ( XM_002936346.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936392.2 Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.

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CloneID	OXa07362
Clone ID Related Accession (Same CDS sequence)	XM_002936346.4 , XM_002936346.5
Accession Version	XM_002936346.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936346.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAT TAGCTGAGCT GTACACTCAG TTCAACAGGG TCTGGATCCC CGACGCAGAG 
GAAGTGTGGC GTTCTGCTGA AATTACAAAA GATCTGAAGA TCGGGGACAC TGTGCTCCAT 
TTGTGTTTGG AAGATGGAAC TCACTTGAAG TATCCAGTGG AACCTGAAAA GCAGGTTCTC 
CCCCCTCTAA GAAATCCAGA TATTCTTGTT GGTGAAAATG ACCTCACAGC CCTCAGTTAT 
TTGCACGAGC CAGCTGTTCT GCACAATCTC AAAGTTCGAT TTGTGGAGTC CAACGTTATT 
TATACATACA GTGGTATTAT CTTGGTTGCA ATCAATCCAT ACAAGGAGCT CCCAATCTAT 
GGAGATGCAA TTATCCATGC ATACAGTGGG CAAAATATGG GAGACATAGA TCCGCATATA 
TTTGCTGTTG CAGAAGAGGC CTACAAACAA ATGGCAAGGA ACAATAGAAA TCAGTCCATT 
ATTGTTAGTG GAGAATCTGG AGCAGGAAAG ACAGTGTCAG CTCGATATAC TATGAGGTAC 
TTTGCCACAG TCAGTAAAAC AAGCAGCAAA GCACACGTGG AAGATAAAGT GCTGGCATCC 
AATCCAATAA CAGAGGCTAT TGGAAATGCA AAAACCACTC GAAATGATAA CAGCAGTCGC 
TTTGGAAAGT ATACTGAAAT CAGTTTTGAT AGAAGGTATA AAATTATTGG TGCAAACTTG 
AGAACGTATC TTCTGGAAAA ATCCCGGGTA GTCTTTCAGT CAGAAAATGA AAGGAATTAC 
CACATATTTT ACCAGCTTTG TGCTTCTGCC AACAAACCGG AATTTGAACA CCTTCATCTA 
ACGAGAGCAG AAGAGTTCAA CTACACTTCA ATGGGTGGCT GCACAGTTAT AACGGGAGTT 
GATGACAAAA AAGATATGTT GGATACACAA AAGACATTTG CTTTATTAGG TTTAGGCACA 
GACTTTCAGA TGGATGTATT TAAAGTCGTA GCAGCTATCC TGCACTTAGG AAATGTGGAG 
ATCAAGCGTG TCAGTGATGA TAGATCCTCT GTTGATGTAA ATGACAAACA CTTGAAGATA 
TTTTGTGATC TTCTGAATCT AGAAGCCTCA AAGGTGGCAC AGTGGCTGTG CAACCGGAGA 
ATTGTGACTG TATCAGACAC TGTTATAAAA CCCATGACCA AACAGCAAGC TGACAATGCA 
AGGGATGCTT TGGCCAAGAA AATCTACTCT CACCTTTTTG ATTATGTCAT CGATAAAATA 
AATAATGCAT TACAGTACCC AGGGAAACAA CATACATTTA TAGGTGTCCT TGATATTTAT 
GGGTTCGAAA CATTTGAAAT TAACAGTTTT GAACAGTTTT GCATCAATTA TGCCAATGAG 
AAGCTGCAGC AACAGTTCAA CCTTCATGTT TTCAAGCTTG AGCAGGAAGA ATATATGAAG 
GAAGACATCC CATGGACTCT TATTGACTTT TATGATAACC AAATAGTAAT TGACCTCATT 
GAAGCAAAGA TGGGCATTTT GGACCTGCTA GATGAAGAGT GCCTGCTGCC GCAAGGAACA 
GACGAAAACT GGTTACAGAA GCTGTATAAT AACTACCTTA ACAAGAACCC CTTGTTTGAA 
AAGCCCCGAA TGTCCAACAG ATCATTCATT ATACAGCACT TTGCAGATAA GGTAGAATAT 
AAGTGTGATG GATTTTTGGA GAAAAACCGA GACACTGTGT ATGATGTTTT AATTGATCTT 
CTGAGGACTA GTAAGTTTCA GCTGTGCTCC AGCTTTTTTC AAGACAACAC AAGCAAGTCA 
GCACAATTCA GTTCTGGAAT CCAGGTGAAA TCTGCAAGAC CTGCGATAAA ATCATCAAAT 
AAAGAGTTCA GAATGACTGT GGGAAGCAAG TTTCGGAACT CGTTATGTTT GCTCATGGAG 
ACCCTTAATG CAACAACACC ACACTATGTA CGATGCATAA AGCCCAACGA TTTAAAACTT 
CCATTTGAGT TTGATGCCAA AAGAGTAGTT CAACAGCTAC GAGCCTGTGG AGTTCTAGAA 
ACCATAAGAA TCAGTGCACA AAGCTACCCT TCCAGGTGGA CATACATTGA GTTCTTTGGC 
CGTTACAGCA TTCTTATGAC TCAGCAGGAA CTGTCCCTGA ATGATAAGAA ATACATTTGC 
AAAATAGTAC TGCAAAGACT GATCCAGGAT TCCAACCAAT ATCAGTTTGG AAGAACCAAA 
ATCTTCTTCA GAGCTGGCCA GGTGGCTTAC TTAGAAAAAT TGAGATCAGA CAAGCTAAGG 
AATGCCTGCG TCATAATCCA AAAGAGCGTA CGAGGATGGG TGCAAAAGAA GAAGTTTAGC 
CGGGCCCGGC GTTCGGCCAT TGTCATACAG CAGTATTTCC GGGGCCAGAG AGCTGTTAGG 
CAAGCAATAA GTGGAAAAGC CCTGAAGCAT GCTTGGGCAG CAATCATTAT CCAGAAATAT 
GTTCGGGGAT TCTTAGTCCG TCGCATCTAC CAACTTATCC TGGTGGCCAC AGTGACCATT 
CAGTCATTCA CACGTGGTTA CTTGGCTAGG AAGAGATATC ACAAGATGCG AGAAGAGCAC 
AAGGCTTTAA TACTGCAGAA ATACGCCCGT GCTTGGCTGG CAAGGCGAAG GTTCCAGAAC 
GTCCGTCGTT TTGTGCTCAA CATCCAGCTT TCCTACAGAG TGCAGCGCCT GCAGAAAAAA 
CTTGAAGATC AGAACAAAGA GAACCATGGG CTGTTGGAAC GTCTGACAAG CCTTGCGTCG 
TCACACACAC ACGATGTGGA TAAAGTGCAC AAACTCGAAT CGGAGCTGGA AAAAATTTCC 
TCCCAAAAGA AACATCTGGA TGAAAAGGGA AAGAAGTACA AGGAAGAAGC TGAGGAGAAA 
ATGACAAAGC TTCAAGCCCA AAATGCTGAA CTTCTTGAGC AGAAACATCA AATGGAAGTA 
AAACTCGAGA CCAAAACCCA AGAAATGAAA GATCAGATGG ATCAGCTCAC TAACCAACTA 
TTCACTGATG TGGAGAAAGA AGAAAAACAA AGGATGAAAC TTGAGAAACA TTTTGAAGCC 
CAGGTTACTG AATTCGACAC TATGATAGAA AAATTAAAAG ATGAAATCCA AGTGTTAAAG 
GAGAGGAATA AGCATCTCCT GAAGCAGGCA GAAGAAGAGC AACTTGTCAA TGATGGTTTA 
AGAGAAGAGA TAGACCAACT TTCCATCCAA GTAAAGAAAA TACCAACATT TCAAACAGAT 
ATTGAGCTTC TTGAAAACCA GAAAAGGGAT TTAGAACATC AACTACAGGA ACAAAAGCGT 
GAAATGAGAG AAAAGTTGGA TGAAATGGCA AAACAGCTTC TGAACAGTTT TGAAATAGAG 
GATGTTAAAG CTAGACTGGC TGAAGAGGAA CTAGGAAATC TAAATGAAGA TGGGGAAATA 
TGGTTTGCTT ATGAAGGTCT GAAAAAAGCA ACCAGAATAC TAGAAGCTCA TTTTCAGACA 
CAGAGAGAGA ACTATGAAAA GGATATTGAG TTGTTGAACT TTAAGGTCGA GCACCTCCAT 
GATGACATTA AACAGATACA GAATTCTTAC ATGGAGGAAA AAGACTCAAA GGAGAGCATC 
ACTCTTGAAG TTGAAAGGCT GAGATCTGAT AATAAGGTGG TTAAGGATCT GAGGAAAAAG 
TTGGCTGAAC TTGAGGAACA AAACATGGAC CTACAGGGCC GTCTCTATGA GCAAACTATG 
AAATTGCCTG AAAAATCTAA CCGCAGGAAA AATTATCCTG ATGAGGATGA AGTATCCCTC 
AAACTGTGGA ATGGTCAGAG AGAAAATGAT ATGGCAAATG TCAAAATACA AGCAATTCAG 
ACAGACAATA TGTATATGAA GGAACAAAAT GAAGCAGACA CCAGGGACTT TGAAGAACAG 
GTAGATATGA AAGACCGAAC AATCAGGAAA CTTCAAGATC AGATAAAGGC TCTTACGAAG 
TCCATTGAAA AGGATGATGA AGTAAGGGTG CCAACAGTGT TTAAAGATTA TTTGGGAATG 
CTGGAGTTTG CTTCAGAAGA TGAATCAAAA CTTATCAGGA ATCTTATTCT AGACCTCAAA 
CCTCGTGGTG TTGTAGTCAA CATGATCCCA GGCCTGCCAG CTCACATTAT GTTCATGTGT 
GTGAGGTACA CAGACTACAT AAACAATGCT GAAAGAATAA AATCTCTGAT GAATGCTTCC 
ATTAACAGCA TCAAACAGGT TATTAAGGAA CATGCAGATG ATTTTGAGAT GTTGTCATTC 
TGGCTTTCCA ATGTCCACCA TTTTCTAAAC TGCTTAAAGC AGTACAGCGG GGAAGAGGAA 
TTTATGAAAT ATAACCAGCC CCTTCAGAAT AAGAACTGCT TAAAGAATTT TGATCTGTCA 
GAATATAGAC AAATTATCAG CGATTTGGCA ATCCGGATAT ATCATCAGTT CATTACTGTA 
ATGGAGAACA ACCTTCAGCC AATGATAGTG CCTGGCATGC TGGAATATGA AAGCCTTCAT 
GGAATTTCAG TCTCCAAACC AACAGGATTC CGTAAACGTT CATCTAGTAT AGATGACACA 
GACTCTTACA CTATGACCTC AATTCTACAG CAGCTTAGTT ATTTTTATAC GACTATGTGC 
CAGCATGGCA TGGACCCTGA GCTCCTGAAA CAAACAATGA AGCAGCTTTT TTTCCTGATT 
GCCACTGTCA CCATCAACAG TCTTTTCCTT CGCAAGGACA TGTGCTCGTG TAGAAAAGGA 
ATGCAGATTC GGTGTAATAT CAGTTACCTT GAAGAATGGC TGCAAGGCAA GAATCTTCAG 
GGGGCTACAG CCAAAGAAAC ATTGGAGCCC CTTTCCCAGG CAGCATGGCT ATTACAAGTA 
AAAAAGATTA CAGATGATGA CGCCAAAGAA ATCTATGAAC GTTGTACTGC ACTTTCATCT 
GTGCAGATTG TAAAGATCCT TAACTCCTAC ACTCCAATTG ATGATTTTGA GAAACGCGTA 
ACGCCATCCT TTGTACGCAA GGTCCAGTCC ATGCTAAACA ACAGAGAAGA CTGCTCTCAG 
TTAATGCTGG ATAGTAAATA CCTCTTCCAA GTGACATTTC CTTTCAGCCC ATCTCCCCAC 
GCACTGGAGA TGATTCAAAT TCCCAGCAGT CTGAAACTTG GCTTCCTAAC AAGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936392.2
CDS	254..5413
Translation	MALLAELYTQFNRVWIPDAEEVWRSAEITKDLKIGDTVLHLCLEDGTHLKYPVEPEKQVLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESNVIYTYSGIILVAINPYKELPIYGDAIIHAYSGQNMGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_002936346.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936346.5

ORF Insert Sequence:

ATGGCGCTAT TAGCTGAGCT GTACACTCAG TTCAACAGGG TCTGGATCCC CGACGCAGAG 
GAAGTGTGGC GTTCTGCTGA AATTACAAAA GATCTGAAGA TCGGGGACAC TGTGCTCCAT 
TTGTGTTTGG AAGATGGAAC TCACTTGAAG TATCCAGTGG AACCTGAAAA GCAGGTTCTC 
CCCCCTCTAA GAAATCCAGA TATTCTTGTT GGTGAAAATG ACCTCACAGC CCTCAGTTAT 
TTGCACGAGC CAGCTGTTCT GCACAATCTC AAAGTTCGAT TTGTGGAGTC CAACGTTATT 
TATACATACA GTGGTATTAT CTTGGTTGCA ATCAATCCAT ACAAGGAGCT CCCAATCTAT 
GGAGATGCAA TTATCCATGC ATACAGTGGG CAAAATATGG GAGACATAGA TCCGCATATA 
TTTGCTGTTG CAGAAGAGGC CTACAAACAA ATGGCAAGGA ACAATAGAAA TCAGTCCATT 
ATTGTTAGTG GAGAATCTGG AGCAGGAAAG ACAGTGTCAG CTCGATATAC TATGAGGTAC 
TTTGCCACAG TCAGTAAAAC AAGCAGCAAA GCACACGTGG AAGATAAAGT GCTGGCATCC 
AATCCAATAA CAGAGGCTAT TGGAAATGCA AAAACCACTC GAAATGATAA CAGCAGTCGC 
TTTGGAAAGT ATACTGAAAT CAGTTTTGAT AGAAGGTATA AAATTATTGG TGCAAACTTG 
AGAACGTATC TTCTGGAAAA ATCCCGGGTA GTCTTTCAGT CAGAAAATGA AAGGAATTAC 
CACATATTTT ACCAGCTTTG TGCTTCTGCC AACAAACCGG AATTTGAACA CCTTCATCTA 
ACGAGAGCAG AAGAGTTCAA CTACACTTCA ATGGGTGGCT GCACAGTTAT AACGGGAGTT 
GATGACAAAA AAGATATGTT GGATACACAA AAGACATTTG CTTTATTAGG TTTAGGCACA 
GACTTTCAGA TGGATGTATT TAAAGTCGTA GCAGCTATCC TGCACTTAGG AAATGTGGAG 
ATCAAGCGTG TCAGTGATGA TAGATCCTCT GTTGATGTAA ATGACAAACA CTTGAAGATA 
TTTTGTGATC TTCTGAATCT AGAAGCCTCA AAGGTGGCAC AGTGGCTGTG CAACCGGAGA 
ATTGTGACTG TATCAGACAC TGTTATAAAA CCCATGACCA AACAGCAAGC TGACAATGCA 
AGGGATGCTT TGGCCAAGAA AATCTACTCT CACCTTTTTG ATTATGTCAT CGATAAAATA 
AATAATGCAT TACAGTACCC AGGGAAACAA CATACATTTA TAGGTGTCCT TGATATTTAT 
GGGTTCGAAA CATTTGAAAT TAACAGTTTT GAACAGTTTT GCATCAATTA TGCCAATGAG 
AAGCTGCAGC AACAGTTCAA CCTTCATGTT TTCAAGCTTG AGCAGGAAGA ATATATGAAG 
GAAGACATCC CATGGACTCT TATTGACTTT TATGATAACC AAATAGTAAT TGACCTCATT 
GAAGCAAAGA TGGGCATTTT GGACCTGCTA GATGAAGAGT GCCTGCTGCC GCAAGGAACA 
GACGAAAACT GGTTACAGAA GCTGTATAAT AACTACCTTA ACAAGAACCC CTTGTTTGAA 
AAGCCCCGAA TGTCCAACAG ATCATTCATT ATACAGCACT TTGCAGATAA GGTAGAATAT 
AAGTGTGATG GATTTTTGGA GAAAAACCGA GACACTGTGT ATGATGTTTT AATTGATCTT 
CTGAGGACTA GTAAGTTTCA GCTGTGCTCC AGCTTTTTTC AAGACAACAC AAGCAAGTCA 
GCACAATTCA GTTCTGGAAT CCAGGTGAAA TCTGCAAGAC CTGCGATAAA ATCATCAAAT 
AAAGAGTTCA GAATGACTGT GGGAAGCAAG TTTCGGAACT CGTTATGTTT GCTCATGGAG 
ACCCTTAATG CAACAACACC ACACTATGTA CGATGCATAA AGCCCAACGA TTTAAAACTT 
CCATTTGAGT TTGATGCCAA AAGAGTAGTT CAACAGCTAC GAGCCTGTGG AGTTCTAGAA 
ACCATAAGAA TCAGTGCACA AAGCTACCCT TCCAGGTGGA CATACATTGA GTTCTTTGGC 
CGTTACAGCA TTCTTATGAC TCAGCAGGAA CTGTCCCTGA ATGATAAGAA ATACATTTGC 
AAAATAGTAC TGCAAAGACT GATCCAGGAT TCCAACCAAT ATCAGTTTGG AAGAACCAAA 
ATCTTCTTCA GAGCTGGCCA GGTGGCTTAC TTAGAAAAAT TGAGATCAGA CAAGCTAAGG 
AATGCCTGCG TCATAATCCA AAAGAGCGTA CGAGGATGGG TGCAAAAGAA GAAGTTTAGC 
CGGGCCCGGC GTTCGGCCAT TGTCATACAG CAGTATTTCC GGGGCCAGAG AGCTGTTAGG 
CAAGCAATAA GTGGAAAAGC CCTGAAGCAT GCTTGGGCAG CAATCATTAT CCAGAAATAT 
GTTCGGGGAT TCTTAGTCCG TCGCATCTAC CAACTTATCC TGGTGGCCAC AGTGACCATT 
CAGTCATTCA CACGTGGTTA CTTGGCTAGG AAGAGATATC ACAAGATGCG AGAAGAGCAC 
AAGGCTTTAA TACTGCAGAA ATACGCCCGT GCTTGGCTGG CAAGGCGAAG GTTCCAGAAC 
GTCCGTCGTT TTGTGCTCAA CATCCAGCTT TCCTACAGAG TGCAGCGCCT GCAGAAAAAA 
CTTGAAGATC AGAACAAAGA GAACCATGGG CTGTTGGAAC GTCTGACAAG CCTTGCGTCG 
TCACACACAC ACGATGTGGA TAAAGTGCAC AAACTCGAAT CGGAGCTGGA AAAAATTTCC 
TCCCAAAAGA AACATCTGGA TGAAAAGGGA AAGAAGTACA AGGAAGAAGC TGAGGAGAAA 
ATGACAAAGC TTCAAGCCCA AAATGCTGAA CTTCTTGAGC AGAAACATCA AATGGAAGTA 
AAACTCGAGA CCAAAACCCA AGAAATGAAA GATCAGATGG ATCAGCTCAC TAACCAACTA 
TTCACTGATG TGGAGAAAGA AGAAAAACAA AGGATGAAAC TTGAGAAACA TTTTGAAGCC 
CAGGTTACTG AATTCGACAC TATGATAGAA AAATTAAAAG ATGAAATCCA AGTGTTAAAG 
GAGAGGAATA AGCATCTCCT GAAGCAGGCA GAAGAAGAGC AACTTGTCAA TGATGGTTTA 
AGAGAAGAGA TAGACCAACT TTCCATCCAA GTAAAGAAAA TACCAACATT TCAAACAGAT 
ATTGAGCTTC TTGAAAACCA GAAAAGGGAT TTAGAACATC AACTACAGGA ACAAAAGCGT 
GAAATGAGAG AAAAGTTGGA TGAAATGGCA AAACAGCTTC TGAACAGTTT TGAAATAGAG 
GATGTTAAAG CTAGACTGGC TGAAGAGGAA CTAGGAAATC TAAATGAAGA TGGGGAAATA 
TGGTTTGCTT ATGAAGGTCT GAAAAAAGCA ACCAGAATAC TAGAAGCTCA TTTTCAGACA 
CAGAGAGAGA ACTATGAAAA GGATATTGAG TTGTTGAACT TTAAGGTCGA GCACCTCCAT 
GATGACATTA AACAGATACA GAATTCTTAC ATGGAGGAAA AAGACTCAAA GGAGAGCATC 
ACTCTTGAAG TTGAAAGGCT GAGATCTGAT AATAAGGTGG TTAAGGATCT GAGGAAAAAG 
TTGGCTGAAC TTGAGGAACA AAACATGGAC CTACAGGGCC GTCTCTATGA GCAAACTATG 
AAATTGCCTG AAAAATCTAA CCGCAGGAAA AATTATCCTG ATGAGGATGA AGTATCCCTC 
AAACTGTGGA ATGGTCAGAG AGAAAATGAT ATGGCAAATG TCAAAATACA AGCAATTCAG 
ACAGACAATA TGTATATGAA GGAACAAAAT GAAGCAGACA CCAGGGACTT TGAAGAACAG 
GTAGATATGA AAGACCGAAC AATCAGGAAA CTTCAAGATC AGATAAAGGC TCTTACGAAG 
TCCATTGAAA AGGATGATGA AGTAAGGGTG CCAACAGTGT TTAAAGATTA TTTGGGAATG 
CTGGAGTTTG CTTCAGAAGA TGAATCAAAA CTTATCAGGA ATCTTATTCT AGACCTCAAA 
CCTCGTGGTG TTGTAGTCAA CATGATCCCA GGCCTGCCAG CTCACATTAT GTTCATGTGT 
GTGAGGTACA CAGACTACAT AAACAATGCT GAAAGAATAA AATCTCTGAT GAATGCTTCC 
ATTAACAGCA TCAAACAGGT TATTAAGGAA CATGCAGATG ATTTTGAGAT GTTGTCATTC 
TGGCTTTCCA ATGTCCACCA TTTTCTAAAC TGCTTAAAGC AGTACAGCGG GGAAGAGGAA 
TTTATGAAAT ATAACCAGCC CCTTCAGAAT AAGAACTGCT TAAAGAATTT TGATCTGTCA 
GAATATAGAC AAATTATCAG CGATTTGGCA ATCCGGATAT ATCATCAGTT CATTACTGTA 
ATGGAGAACA ACCTTCAGCC AATGATAGTG CCTGGCATGC TGGAATATGA AAGCCTTCAT 
GGAATTTCAG TCTCCAAACC AACAGGATTC CGTAAACGTT CATCTAGTAT AGATGACACA 
GACTCTTACA CTATGACCTC AATTCTACAG CAGCTTAGTT ATTTTTATAC GACTATGTGC 
CAGCATGGCA TGGACCCTGA GCTCCTGAAA CAAACAATGA AGCAGCTTTT TTTCCTGATT 
GCCACTGTCA CCATCAACAG TCTTTTCCTT CGCAAGGACA TGTGCTCGTG TAGAAAAGGA 
ATGCAGATTC GGTGTAATAT CAGTTACCTT GAAGAATGGC TGCAAGGCAA GAATCTTCAG 
GGGGCTACAG CCAAAGAAAC ATTGGAGCCC CTTTCCCAGG CAGCATGGCT ATTACAAGTA 
AAAAAGATTA CAGATGATGA CGCCAAAGAA ATCTATGAAC GTTGTACTGC ACTTTCATCT 
GTGCAGATTG TAAAGATCCT TAACTCCTAC ACTCCAATTG ATGATTTTGA GAAACGCGTA 
ACGCCATCCT TTGTACGCAA GGTCCAGTCC ATGCTAAACA ACAGAGAAGA CTGCTCTCAG 
TTAATGCTGG ATAGTAAATA CCTCTTCCAA GTGACATTTC CTTTCAGCCC ATCTCCCCAC 
GCACTGGAGA TGATTCAAAT TCCCAGCAGT CTGAAACTTG GCTTCCTAAC AAGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5c ( XM_012959596.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815050.1 Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.

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CloneID	OXa36221
Clone ID Related Accession (Same CDS sequence)	XM_012959596.2 , XM_012959596.3
Accession Version	XM_012959596.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACATA GTGACCCCCA GCACAGCAAG CAAAAGTTCA ACAGGGTCTG GATCCCCGAC 
GCAGAGGAAG TGTGGCGTTC TGCTGAAATT ACAAAAGATC TGAAGATCGG GGACACTGTG 
CTCCATTTGT GTTTGGAAGA TGGAACTCAC TTGAAGTATC CAGTGGAACC TGAAAAGCAG 
GTTCTCCCCC CTCTAAGAAA TCCAGATATT CTTGTTGGTG AAAATGACCT CACAGCCCTC 
AGTTATTTGC ACGAGCCAGC TGTTCTGCAC AATCTCAAAG TTCGATTTGT GGAGTCCAAC 
GTTATTTATA CATACAGTGG TATTATCTTG GTTGCAATCA ATCCATACAA GGAGCTCCCA 
ATCTATGGAG ATGCAATTAT CCATGCATAC AGTGGGCAAA ATATGGGAGA CATAGATCCG 
CATATATTTG CTGTTGCAGA AGAGGCCTAC AAACAAATGG CAAGGAACAA TAGAAATCAG 
TCCATTATTG TTAGTGGAGA ATCTGGAGCA GGAAAGACAG TGTCAGCTCG ATATACTATG 
AGGTACTTTG CCACAGTCAG TAAAACAAGC AGCAAAGCAC ACGTGGAAGA TAAAGTGCTG 
GCATCCAATC CAATAACAGA GGCTATTGGA AATGCAAAAA CCACTCGAAA TGATAACAGC 
AGTCGCTTTG GAAAGTATAC TGAAATCAGT TTTGATAGAA GGTATAAAAT TATTGGTGCA 
AACTTGAGAA CGTATCTTCT GGAAAAATCC CGGGTAGTCT TTCAGTCAGA AAATGAAAGG 
AATTACCACA TATTTTACCA GCTTTGTGCT TCTGCCAACA AACCGGAATT TGAACACCTT 
CATCTAACGA GAGCAGAAGA GTTCAACTAC ACTTCAATGG GTGGCTGCAC AGTTATAACG 
GGAGTTGATG ACAAAAAAGA TATGTTGGAT ACACAAAAGA CATTTGCTTT ATTAGGTTTA 
GGCACAGACT TTCAGATGGA TGTATTTAAA GTCGTAGCAG CTATCCTGCA CTTAGGAAAT 
GTGGAGATCA AGCGTGTCAG TGATGATAGA TCCTCTGTTG ATGTAAATGA CAAACACTTG 
AAGATATTTT GTGATCTTCT GAATCTAGAA GCCTCAAAGG TGGCACAGTG GCTGTGCAAC 
CGGAGAATTG TGACTGTATC AGACACTGTT ATAAAACCCA TGACCAAACA GCAAGCTGAC 
AATGCAAGGG ATGCTTTGGC CAAGAAAATC TACTCTCACC TTTTTGATTA TGTCATCGAT 
AAAATAAATA ATGCATTACA GTACCCAGGG AAACAACATA CATTTATAGG TGTCCTTGAT 
ATTTATGGGT TCGAAACATT TGAAATTAAC AGTTTTGAAC AGTTTTGCAT CAATTATGCC 
AATGAGAAGC TGCAGCAACA GTTCAACCTT CATGTTTTCA AGCTTGAGCA GGAAGAATAT 
ATGAAGGAAG ACATCCCATG GACTCTTATT GACTTTTATG ATAACCAAAT AGTAATTGAC 
CTCATTGAAG CAAAGATGGG CATTTTGGAC CTGCTAGATG AAGAGTGCCT GCTGCCGCAA 
GGAACAGACG AAAACTGGTT ACAGAAGCTG TATAATAACT ACCTTAACAA GAACCCCTTG 
TTTGAAAAGC CCCGAATGTC CAACAGATCA TTCATTATAC AGCACTTTGC AGATAAGGTA 
GAATATAAGT GTGATGGATT TTTGGAGAAA AACCGAGACA CTGTGTATGA TGTTTTAATT 
GATCTTCTGA GGACTAGTAA GTTTCAGCTG TGCTCCAGCT TTTTTCAAGA CAACACAAGC 
AAGTCAGCAC AATTCAGTTC TGGAATCCAG GTGAAATCTG CAAGACCTGC GATAAAATCA 
TCAAATAAAG AGTTCAGAAT GACTGTGGGA AGCAAGTTTC GGAACTCGTT ATGTTTGCTC 
ATGGAGACCC TTAATGCAAC AACACCACAC TATGTACGAT GCATAAAGCC CAACGATTTA 
AAACTTCCAT TTGAGTTTGA TGCCAAAAGA GTAGTTCAAC AGCTACGAGC CTGTGGAGTT 
CTAGAAACCA TAAGAATCAG TGCACAAAGC TACCCTTCCA GGTGGACATA CATTGAGTTC 
TTTGGCCGTT ACAGCATTCT TATGACTCAG CAGGAACTGT CCCTGAATGA TAAGAAATAC 
ATTTGCAAAA TAGTACTGCA AAGACTGATC CAGGATTCCA ACCAATATCA GTTTGGAAGA 
ACCAAAATCT TCTTCAGAGC TGGCCAGGTG GCTTACTTAG AAAAATTGAG ATCAGACAAG 
CTAAGGAATG CCTGCGTCAT AATCCAAAAG AGCGTACGAG GATGGGTGCA AAAGAAGAAG 
TTTAGCCGGG CCCGGCGTTC GGCCATTGTC ATACAGCAGT ATTTCCGGGG CCAGAGAGCT 
GTTAGGCAAG CAATAAGTGG AAAAGCCCTG AAGCATGCTT GGGCAGCAAT CATTATCCAG 
AAATATGTTC GGGGATTCTT AGTCCGTCGC ATCTACCAAC TTATCCTGGT GGCCACAGTG 
ACCATTCAGT CATTCACACG TGGTTACTTG GCTAGGAAGA GATATCACAA GATGCGAGAA 
GAGCACAAGG CTTTAATACT GCAGAAATAC GCCCGTGCTT GGCTGGCAAG GCGAAGGTTC 
CAGAACGTCC GTCGTTTTGT GCTCAACATC CAGCTTTCCT ACAGAGTGCA GCGCCTGCAG 
AAAAAACTTG AAGATCAGAA CAAAGAGAAC CATGGGCTGT TGGAACGTCT GACAAGCCTT 
GCGTCGTCAC ACACACACGA TGTGGATAAA GTGCACAAAC TCGAATCGGA GCTGGAAAAA 
ATTTCCTCCC AAAAGAAACA TCTGGATGAA AAGGGAAAGA AGTACAAGGA AGAAGCTGAG 
GAGAAAATGA CAAAGCTTCA AGCCCAAAAT GCTGAACTTC TTGAGCAGAA ACATCAAATG 
GAAGTAAAAC TCGAGACCAA AACCCAAGAA ATGAAAGATC AGATGGATCA GCTCACTAAC 
CAACTATTCA CTGATGTGGA GAAAGAAGAA AAACAAAGGA TGAAACTTGA GAAACATTTT 
GAAGCCCAGG TTACTGAATT CGACACTATG ATAGAAAAAT TAAAAGATGA AATCCAAGTG 
TTAAAGGAGA GGAATAAGCA TCTCCTGAAG CAGGCAGAAG AAGAGCAACT TGTCAATGAT 
GGTTTAAGAG AAGAGATAGA CCAACTTTCC ATCCAAGTAA AGAAAATACC AACATTTCAA 
ACAGATATTG AGCTTCTTGA AAACCAGAAA AGGGATTTAG AACATCAACT ACAGGAACAA 
AAGCGTGAAA TGAGAGAAAA GTTGGATGAA ATGGCAAAAC AGCTTCTGAA CAGTTTTGAA 
ATAGAGGATG TTAAAGCTAG ACTGGCTGAA GAGGAACTAG GAAATCTAAA TGAAGATGGG 
GAAATATGGT TTGCTTATGA AGGTCTGAAA AAAGCAACCA GAATACTAGA AGCTCATTTT 
CAGACACAGA GAGAGAACTA TGAAAAGGAT ATTGAGTTGT TGAACTTTAA GGTCGAGCAC 
CTCCATGATG ACATTAAACA GATACAGAAT TCTTACATGG AGGAAAAAGA CTCAAAGGAG 
AGCATCACTC TTGAAGTTGA AAGGCTGAGA TCTGATAATA AGGTGGTTAA GGATCTGAGG 
AAAAAGTTGG CTGAACTTGA GGAACAAAAC ATGGACCTAC AGGGCCGTCT CTATGAGCAA 
ACTATGAAAT TGCCTGAAAA ATCTAACCGC AGGAAAAATT ATCCTGATGA GGATGAAGTA 
TCCCTCAAAC TGTGGAATGG TCAGAGAGAA AATGATATGG CAAATGTCAA AATACAAGCA 
ATTCAGACAG ACAATATGTA TATGAAGGAA CAAAATGAAG CAGACACCAG GGACTTTGAA 
GAACAGGTAG ATATGAAAGA CCGAACAATC AGGAAACTTC AAGATCAGAT AAAGGCTCTT 
ACGAAGTCCA TTGAAAAGGA TGATGAAGTA AGGGTGCCAA CAGTGTTTAA AGATTATTTG 
GGAATGCTGG AGTTTGCTTC AGAAGATGAA TCAAAACTTA TCAGGAATCT TATTCTAGAC 
CTCAAACCTC GTGGTGTTGT AGTCAACATG ATCCCAGGCC TGCCAGCTCA CATTATGTTC 
ATGTGTGTGA GGTACACAGA CTACATAAAC AATGCTGAAA GAATAAAATC TCTGATGAAT 
GCTTCCATTA ACAGCATCAA ACAGGTTATT AAGGAACATG CAGATGATTT TGAGATGTTG 
TCATTCTGGC TTTCCAATGT CCACCATTTT CTAAACTGCT TAAAGCAGTA CAGCGGGGAA 
GAGGAATTTA TGAAATATAA CCAGCCCCTT CAGAATAAGA ACTGCTTAAA GAATTTTGAT 
CTGTCAGAAT ATAGACAAAT TATCAGCGAT TTGGCAATCC GGATATATCA TCAGTTCATT 
ACTGTAATGG AGAACAACCT TCAGCCAATG ATAGTGCCTG GCATGCTGGA ATATGAAAGC 
CTTCATGGAA TTTCAGTCTC CAAACCAACA GGATTCCGTA AACGTTCATC TAGTATAGAT 
GACACAGACT CTTACACTAT GACCTCAATT CTACAGCAGC TTAGTTATTT TTATACGACT 
ATGTGCCAGC ATGGCATGGA CCCTGAGCTC CTGAAACAAA CAATGAAGCA GCTTTTTTTC 
CTGATTGCCA CTGTCACCAT CAACAGTCTT TTCCTTCGCA AGGACATGTG CTCGTGTAGA 
AAAGGAATGC AGATTCGGTG TAATATCAGT TACCTTGAAG AATGGCTGCA AGGCAAGAAT 
CTTCAGGGGG CTACAGCCAA AGAAACATTG GAGCCCCTTT CCCAGGCAGC ATGGCTATTA 
CAAGTAAAAA AGATTACAGA TGATGACGCC AAAGAAATCT ATGAACGTTG TACTGCACTT 
TCATCTGTGC AGATTGTAAA GATCCTTAAC TCCTACACTC CAATTGATGA TTTTGAGAAA 
CGCGTAACGC CATCCTTTGT ACGCAAGGTC CAGTCCATGC TAAACAACAG AGAAGACTGC 
TCTCAGTTAA TGCTGGATAG TAAATACCTC TTCCAAGTGA CATTTCCTTT CAGCCCATCT 
CCCCACGCAC TGGAGATGAT TCAAATTCCC AGCAGTCTGA AACTTGGCTT CCTAACAAGA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815050.1
CDS	53..5218
Translation	MLHSDPQHSKQKFNRVWIPDAEEVWRSAEITKDLKIGDTVLHLCLEDGTHLKYPVEPEKQVLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESNVIYTYSGIILVAINPYKELPIYGDAIIHAYSGQNMGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_012959596.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959596.3

ORF Insert Sequence:

ATGCTACATA GTGACCCCCA GCACAGCAAG CAAAAGTTCA ACAGGGTCTG GATCCCCGAC 
GCAGAGGAAG TGTGGCGTTC TGCTGAAATT ACAAAAGATC TGAAGATCGG GGACACTGTG 
CTCCATTTGT GTTTGGAAGA TGGAACTCAC TTGAAGTATC CAGTGGAACC TGAAAAGCAG 
GTTCTCCCCC CTCTAAGAAA TCCAGATATT CTTGTTGGTG AAAATGACCT CACAGCCCTC 
AGTTATTTGC ACGAGCCAGC TGTTCTGCAC AATCTCAAAG TTCGATTTGT GGAGTCCAAC 
GTTATTTATA CATACAGTGG TATTATCTTG GTTGCAATCA ATCCATACAA GGAGCTCCCA 
ATCTATGGAG ATGCAATTAT CCATGCATAC AGTGGGCAAA ATATGGGAGA CATAGATCCG 
CATATATTTG CTGTTGCAGA AGAGGCCTAC AAACAAATGG CAAGGAACAA TAGAAATCAG 
TCCATTATTG TTAGTGGAGA ATCTGGAGCA GGAAAGACAG TGTCAGCTCG ATATACTATG 
AGGTACTTTG CCACAGTCAG TAAAACAAGC AGCAAAGCAC ACGTGGAAGA TAAAGTGCTG 
GCATCCAATC CAATAACAGA GGCTATTGGA AATGCAAAAA CCACTCGAAA TGATAACAGC 
AGTCGCTTTG GAAAGTATAC TGAAATCAGT TTTGATAGAA GGTATAAAAT TATTGGTGCA 
AACTTGAGAA CGTATCTTCT GGAAAAATCC CGGGTAGTCT TTCAGTCAGA AAATGAAAGG 
AATTACCACA TATTTTACCA GCTTTGTGCT TCTGCCAACA AACCGGAATT TGAACACCTT 
CATCTAACGA GAGCAGAAGA GTTCAACTAC ACTTCAATGG GTGGCTGCAC AGTTATAACG 
GGAGTTGATG ACAAAAAAGA TATGTTGGAT ACACAAAAGA CATTTGCTTT ATTAGGTTTA 
GGCACAGACT TTCAGATGGA TGTATTTAAA GTCGTAGCAG CTATCCTGCA CTTAGGAAAT 
GTGGAGATCA AGCGTGTCAG TGATGATAGA TCCTCTGTTG ATGTAAATGA CAAACACTTG 
AAGATATTTT GTGATCTTCT GAATCTAGAA GCCTCAAAGG TGGCACAGTG GCTGTGCAAC 
CGGAGAATTG TGACTGTATC AGACACTGTT ATAAAACCCA TGACCAAACA GCAAGCTGAC 
AATGCAAGGG ATGCTTTGGC CAAGAAAATC TACTCTCACC TTTTTGATTA TGTCATCGAT 
AAAATAAATA ATGCATTACA GTACCCAGGG AAACAACATA CATTTATAGG TGTCCTTGAT 
ATTTATGGGT TCGAAACATT TGAAATTAAC AGTTTTGAAC AGTTTTGCAT CAATTATGCC 
AATGAGAAGC TGCAGCAACA GTTCAACCTT CATGTTTTCA AGCTTGAGCA GGAAGAATAT 
ATGAAGGAAG ACATCCCATG GACTCTTATT GACTTTTATG ATAACCAAAT AGTAATTGAC 
CTCATTGAAG CAAAGATGGG CATTTTGGAC CTGCTAGATG AAGAGTGCCT GCTGCCGCAA 
GGAACAGACG AAAACTGGTT ACAGAAGCTG TATAATAACT ACCTTAACAA GAACCCCTTG 
TTTGAAAAGC CCCGAATGTC CAACAGATCA TTCATTATAC AGCACTTTGC AGATAAGGTA 
GAATATAAGT GTGATGGATT TTTGGAGAAA AACCGAGACA CTGTGTATGA TGTTTTAATT 
GATCTTCTGA GGACTAGTAA GTTTCAGCTG TGCTCCAGCT TTTTTCAAGA CAACACAAGC 
AAGTCAGCAC AATTCAGTTC TGGAATCCAG GTGAAATCTG CAAGACCTGC GATAAAATCA 
TCAAATAAAG AGTTCAGAAT GACTGTGGGA AGCAAGTTTC GGAACTCGTT ATGTTTGCTC 
ATGGAGACCC TTAATGCAAC AACACCACAC TATGTACGAT GCATAAAGCC CAACGATTTA 
AAACTTCCAT TTGAGTTTGA TGCCAAAAGA GTAGTTCAAC AGCTACGAGC CTGTGGAGTT 
CTAGAAACCA TAAGAATCAG TGCACAAAGC TACCCTTCCA GGTGGACATA CATTGAGTTC 
TTTGGCCGTT ACAGCATTCT TATGACTCAG CAGGAACTGT CCCTGAATGA TAAGAAATAC 
ATTTGCAAAA TAGTACTGCA AAGACTGATC CAGGATTCCA ACCAATATCA GTTTGGAAGA 
ACCAAAATCT TCTTCAGAGC TGGCCAGGTG GCTTACTTAG AAAAATTGAG ATCAGACAAG 
CTAAGGAATG CCTGCGTCAT AATCCAAAAG AGCGTACGAG GATGGGTGCA AAAGAAGAAG 
TTTAGCCGGG CCCGGCGTTC GGCCATTGTC ATACAGCAGT ATTTCCGGGG CCAGAGAGCT 
GTTAGGCAAG CAATAAGTGG AAAAGCCCTG AAGCATGCTT GGGCAGCAAT CATTATCCAG 
AAATATGTTC GGGGATTCTT AGTCCGTCGC ATCTACCAAC TTATCCTGGT GGCCACAGTG 
ACCATTCAGT CATTCACACG TGGTTACTTG GCTAGGAAGA GATATCACAA GATGCGAGAA 
GAGCACAAGG CTTTAATACT GCAGAAATAC GCCCGTGCTT GGCTGGCAAG GCGAAGGTTC 
CAGAACGTCC GTCGTTTTGT GCTCAACATC CAGCTTTCCT ACAGAGTGCA GCGCCTGCAG 
AAAAAACTTG AAGATCAGAA CAAAGAGAAC CATGGGCTGT TGGAACGTCT GACAAGCCTT 
GCGTCGTCAC ACACACACGA TGTGGATAAA GTGCACAAAC TCGAATCGGA GCTGGAAAAA 
ATTTCCTCCC AAAAGAAACA TCTGGATGAA AAGGGAAAGA AGTACAAGGA AGAAGCTGAG 
GAGAAAATGA CAAAGCTTCA AGCCCAAAAT GCTGAACTTC TTGAGCAGAA ACATCAAATG 
GAAGTAAAAC TCGAGACCAA AACCCAAGAA ATGAAAGATC AGATGGATCA GCTCACTAAC 
CAACTATTCA CTGATGTGGA GAAAGAAGAA AAACAAAGGA TGAAACTTGA GAAACATTTT 
GAAGCCCAGG TTACTGAATT CGACACTATG ATAGAAAAAT TAAAAGATGA AATCCAAGTG 
TTAAAGGAGA GGAATAAGCA TCTCCTGAAG CAGGCAGAAG AAGAGCAACT TGTCAATGAT 
GGTTTAAGAG AAGAGATAGA CCAACTTTCC ATCCAAGTAA AGAAAATACC AACATTTCAA 
ACAGATATTG AGCTTCTTGA AAACCAGAAA AGGGATTTAG AACATCAACT ACAGGAACAA 
AAGCGTGAAA TGAGAGAAAA GTTGGATGAA ATGGCAAAAC AGCTTCTGAA CAGTTTTGAA 
ATAGAGGATG TTAAAGCTAG ACTGGCTGAA GAGGAACTAG GAAATCTAAA TGAAGATGGG 
GAAATATGGT TTGCTTATGA AGGTCTGAAA AAAGCAACCA GAATACTAGA AGCTCATTTT 
CAGACACAGA GAGAGAACTA TGAAAAGGAT ATTGAGTTGT TGAACTTTAA GGTCGAGCAC 
CTCCATGATG ACATTAAACA GATACAGAAT TCTTACATGG AGGAAAAAGA CTCAAAGGAG 
AGCATCACTC TTGAAGTTGA AAGGCTGAGA TCTGATAATA AGGTGGTTAA GGATCTGAGG 
AAAAAGTTGG CTGAACTTGA GGAACAAAAC ATGGACCTAC AGGGCCGTCT CTATGAGCAA 
ACTATGAAAT TGCCTGAAAA ATCTAACCGC AGGAAAAATT ATCCTGATGA GGATGAAGTA 
TCCCTCAAAC TGTGGAATGG TCAGAGAGAA AATGATATGG CAAATGTCAA AATACAAGCA 
ATTCAGACAG ACAATATGTA TATGAAGGAA CAAAATGAAG CAGACACCAG GGACTTTGAA 
GAACAGGTAG ATATGAAAGA CCGAACAATC AGGAAACTTC AAGATCAGAT AAAGGCTCTT 
ACGAAGTCCA TTGAAAAGGA TGATGAAGTA AGGGTGCCAA CAGTGTTTAA AGATTATTTG 
GGAATGCTGG AGTTTGCTTC AGAAGATGAA TCAAAACTTA TCAGGAATCT TATTCTAGAC 
CTCAAACCTC GTGGTGTTGT AGTCAACATG ATCCCAGGCC TGCCAGCTCA CATTATGTTC 
ATGTGTGTGA GGTACACAGA CTACATAAAC AATGCTGAAA GAATAAAATC TCTGATGAAT 
GCTTCCATTA ACAGCATCAA ACAGGTTATT AAGGAACATG CAGATGATTT TGAGATGTTG 
TCATTCTGGC TTTCCAATGT CCACCATTTT CTAAACTGCT TAAAGCAGTA CAGCGGGGAA 
GAGGAATTTA TGAAATATAA CCAGCCCCTT CAGAATAAGA ACTGCTTAAA GAATTTTGAT 
CTGTCAGAAT ATAGACAAAT TATCAGCGAT TTGGCAATCC GGATATATCA TCAGTTCATT 
ACTGTAATGG AGAACAACCT TCAGCCAATG ATAGTGCCTG GCATGCTGGA ATATGAAAGC 
CTTCATGGAA TTTCAGTCTC CAAACCAACA GGATTCCGTA AACGTTCATC TAGTATAGAT 
GACACAGACT CTTACACTAT GACCTCAATT CTACAGCAGC TTAGTTATTT TTATACGACT 
ATGTGCCAGC ATGGCATGGA CCCTGAGCTC CTGAAACAAA CAATGAAGCA GCTTTTTTTC 
CTGATTGCCA CTGTCACCAT CAACAGTCTT TTCCTTCGCA AGGACATGTG CTCGTGTAGA 
AAAGGAATGC AGATTCGGTG TAATATCAGT TACCTTGAAG AATGGCTGCA AGGCAAGAAT 
CTTCAGGGGG CTACAGCCAA AGAAACATTG GAGCCCCTTT CCCAGGCAGC ATGGCTATTA 
CAAGTAAAAA AGATTACAGA TGATGACGCC AAAGAAATCT ATGAACGTTG TACTGCACTT 
TCATCTGTGC AGATTGTAAA GATCCTTAAC TCCTACACTC CAATTGATGA TTTTGAGAAA 
CGCGTAACGC CATCCTTTGT ACGCAAGGTC CAGTCCATGC TAAACAACAG AGAAGACTGC 
TCTCAGTTAA TGCTGGATAG TAAATACCTC TTCCAAGTGA CATTTCCTTT CAGCCCATCT 
CCCCACGCAC TGGAGATGAT TCAAATTCCC AGCAGTCTGA AACTTGGCTT CCTAACAAGA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5c ( XM_018092428.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947917.1 Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa43364
Clone ID Related Accession (Same CDS sequence)	XM_018092428.1
Accession Version	XM_018092428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACA TAGATCCGCA TATATTTGCT GTTGCAGAAG AGGCCTACAA ACAAATGGCA 
AGGAACAATA GAAATCAGTC CATTATTGTT AGTGGAGAAT CTGGAGCAGG AAAGACAGTG 
TCAGCTCGAT ATACTATGAG GTACTTTGCC ACAGTCAGTA AAACAAGCAG CAAAGCACAC 
GTGGAAGATA AAGTGCTGGC ATCCAATCCA ATAACAGAGG CTATTGGAAA TGCAAAAACC 
ACTCGAAATG ATAACAGCAG TCGCTTTGGA AAGTATACTG AAATCAGTTT TGATAGAAGG 
TATAAAATTA TTGGTGCAAA CTTGAGAACG TATCTTCTGG AAAAATCCCG GGTAGTCTTT 
CAGTCAGAAA ATGAAAGGAA TTACCACATA TTTTACCAGC TTTGTGCTTC TGCCAACAAA 
CCGGAATTTG AACACCTTCA TCTAACGAGA GCAGAAGAGT TCAACTACAC TTCAATGGGT 
GGCTGCACAG TTATAACGGG AGTTGATGAC AAAAAAGATA TGTTGGATAC ACAAAAGACA 
TTTGCTTTAT TAGGTTTAGG CACAGACTTT CAGATGGATG TATTTAAAGT CGTAGCAGCT 
ATCCTGCACT TAGGAAATGT GGAGATCAAG CGTGTCAGTG ATGATAGATC CTCTGTTGAT 
GTAAATGACA AACACTTGAA GATATTTTGT GATCTTCTGA ATCTAGAAGC CTCAAAGGTG 
GCACAGTGGC TGTGCAACCG GAGAATTGTG ACTGTATCAG ACACTGTTAT AAAACCCATG 
ACCAAACAGC AAGCTGACAA TGCAAGGGAT GCTTTGGCCA AGAAAATCTA CTCTCACCTT 
TTTGATTATG TCATCGATAA AATAAATAAT GCATTACAGT ACCCAGGGAA ACAACATACA 
TTTATAGGTG TCCTTGATAT TTATGGGTTC GAAACATTTG AAATTAACAG TTTTGAACAG 
TTTTGCATCA ATTATGCCAA TGAGAAGCTG CAGCAACAGT TCAACCTTCA TGTTTTCAAG 
CTTGAGCAGG AAGAATATAT GAAGGAAGAC ATCCCATGGA CTCTTATTGA CTTTTATGAT 
AACCAAATAG TAATTGACCT CATTGAAGCA AAGATGGGCA TTTTGGACCT GCTAGATGAA 
GAGTGCCTGC TGCCGCAAGG AACAGACGAA AACTGGTTAC AGAAGCTGTA TAATAACTAC 
CTTAACAAGA ACCCCTTGTT TGAAAAGCCC CGAATGTCCA ACAGATCATT CATTATACAG 
CACTTTGCAG ATAAGGTAGA ATATAAGTGT GATGGATTTT TGGAGAAAAA CCGAGACACT 
GTGTATGATG TTTTAATTGA TCTTCTGAGG ACTAGTAAGT TTCAGCTGTG CTCCAGCTTT 
TTTCAAGACA ACACAAGCAA GTCAGCACAA TTCAGTTCTG GAATCCAGGT GAAATCTGCA 
AGACCTGCGA TAAAATCATC AAATAAAGAG TTCAGAATGA CTGTGGGAAG CAAGTTTCGG 
AACTCGTTAT GTTTGCTCAT GGAGACCCTT AATGCAACAA CACCACACTA TGTACGATGC 
ATAAAGCCCA ACGATTTAAA ACTTCCATTT GAGTTTGATG CCAAAAGAGT AGTTCAACAG 
CTACGAGCCT GTGGAGTTCT AGAAACCATA AGAATCAGTG CACAAAGCTA CCCTTCCAGG 
TGGACATACA TTGAGTTCTT TGGCCGTTAC AGCATTCTTA TGACTCAGCA GGAACTGTCC 
CTGAATGATA AGAAATACAT TTGCAAAATA GTACTGCAAA GACTGATCCA GGATTCCAAC 
CAATATCAGT TTGGAAGAAC CAAAATCTTC TTCAGAGCTG GCCAGGTGGC TTACTTAGAA 
AAATTGAGAT CAGACAAGCT AAGGAATGCC TGCGTCATAA TCCAAAAGAG CGTACGAGGA 
TGGGTGCAAA AGAAGAAGTT TAGCCGGGCC CGGCGTTCGG CCATTGTCAT ACAGCAGTAT 
TTCCGGGGCC AGAGAGCTGT TAGGCAAGCA ATAAGTGGAA AAGCCCTGAA GCATGCTTGG 
GCAGCAATCA TTATCCAGAA ATATGTTCGG GGATTCTTAG TCCGTCGCAT CTACCAACTT 
ATCCTGGTGG CCACAGTGAC CATTCAGTCA TTCACACGTG GTTACTTGGC TAGGAAGAGA 
TATCACAAGA TGCGAGAAGA GCACAAGGCT TTAATACTGC AGAAATACGC CCGTGCTTGG 
CTGGCAAGGC GAAGGTTCCA GAACGTCCGT CGTTTTGTGC TCAACATCCA GCTTTCCTAC 
AGAGTGCAGC GCCTGCAGAA AAAACTTGAA GATCAGAACA AAGAGAACCA TGGGCTGTTG 
GAACGTCTGA CAAGCCTTGC GTCGTCACAC ACACACGATG TGGATAAAGT GCACAAACTC 
GAATCGGAGC TGGAAAAAAT TTCCTCCCAA AAGAAACATC TGGATGAAAA GGGAAAGAAG 
TACAAGGAAG AAGCTGAGGA GAAAATGACA AAGCTTCAAG CCCAAAATGC TGAACTTCTT 
GAGCAGAAAC ATCAAATGGA AGTAAAACTC GAGACCAAAA CCCAAGAAAT GAAAGATCAG 
ATGGATCAGC TCACTAACCA ACTATTCACT GATGTGGAGA AAGAAGAAAA ACAAAGGATG 
AAACTTGAGA AACATTTTGA AGCCCAGGTT ACTGAATTCG ACACTATGAT AGAAAAATTA 
AAAGATGAAA TCCAAGTGTT AAAGGAGAGG AATAAGCATC TCCTGAAGCA GGCAGAAGAA 
GAGCAACTTG TCAATGATGG TTTAAGAGAA GAGATAGACC AACTTTCCAT CCAAGTAAAG 
AAAATACCAA CATTTCAAAC AGATATTGAG CTTCTTGAAA ACCAGAAAAG GGATTTAGAA 
CATCAACTAC AGGAACAAAA GCGTGAAATG AGAGAAAAGT TGGATGAAAT GGCAAAACAG 
CTTCTGAACA GTTTTGAAAT AGAGGATGTT AAAGCTAGAC TGGCTGAAGA GGAACTAGGA 
AATCTAAATG AAGATGGGGA AATATGGTTT GCTTATGAAG GTCTGAAAAA AGCAACCAGA 
ATACTAGAAG CTCATTTTCA GACACAGAGA GAGAACTATG AAAAGGATAT TGAGTTGTTG 
AACTTTAAGG TCGAGCACCT CCATGATGAC ATTAAACAGA TACAGAATTC TTACATGGAG 
GAAAAAGACT CAAAGGAGAG CATCACTCTT GAAGTTGAAA GGCTGAGATC TGATAATAAG 
GTGGTTAAGG ATCTGAGGAA AAAGTTGGCT GAACTTGAGG AACAAAACAT GGACCTACAG 
GGCCGTCTCT ATGAGCAAAC TATGAAATTG CCTGAAAAAT CTAACCGCAG GAAAAATTAT 
CCTGATGAGG ATGAAGTATC CCTCAAACTG TGGAATGGTC AGAGAGAAAA TGATATGGCA 
AATGTCAAAA TACAAGCAAT TCAGACAGAC AATATGTATA TGAAGGAACA AAATGAAGCA 
GACACCAGGG ACTTTGAAGA ACAGGTAGAT ATGAAAGACC GAACAATCAG GAAACTTCAA 
GATCAGATAA AGGCTCTTAC GAAGTCCATT GAAAAGGATG ATGAAGTAAG GGTGCCAACA 
GTGTTTAAAG ATTATTTGGG AATGCTGGAG TTTGCTTCAG AAGATGAATC AAAACTTATC 
AGGAATCTTA TTCTAGACCT CAAACCTCGT GGTGTTGTAG TCAACATGAT CCCAGGCCTG 
CCAGCTCACA TTATGTTCAT GTGTGTGAGG TACACAGACT ACATAAACAA TGCTGAAAGA 
ATAAAATCTC TGATGAATGC TTCCATTAAC AGCATCAAAC AGGTTATTAA GGAACATGCA 
GATGATTTTG AGATGTTGTC ATTCTGGCTT TCCAATGTCC ACCATTTTCT AAACTGCTTA 
AAGCAGTACA GCGGGGAAGA GGAATTTATG AAATATAACC AGCCCCTTCA GAATAAGAAC 
TGCTTAAAGA ATTTTGATCT GTCAGAATAT AGACAAATTA TCAGCGATTT GGCAATCCGG 
ATATATCATC AGTTCATTAC TGTAATGGAG AACAACCTTC AGCCAATGAT AGTGCCTGGC 
ATGCTGGAAT ATGAAAGCCT TCATGGAATT TCAGTCTCCA AACCAACAGG ATTCCGTAAA 
CGTTCATCTA GTATAGATGA CACAGACTCT TACACTATGA CCTCAATTCT ACAGCAGCTT 
AGTTATTTTT ATACGACTAT GTGCCAGCAT GGCATGGACC CTGAGCTCCT GAAACAAACA 
ATGAAGCAGC TTTTTTTCCT GATTGCCACT GTCACCATCA ACAGTCTTTT CCTTCGCAAG 
GACATGTGCT CGTGTAGAAA AGGAATGCAG ATTCGGTGTA ATATCAGTTA CCTTGAAGAA 
TGGCTGCAAG GCAAGAATCT TCAGGGGGCT ACAGCCAAAG AAACATTGGA GCCCCTTTCC 
CAGGCAGCAT GGCTATTACA AGTAAAAAAG ATTACAGATG ATGACGCCAA AGAAATCTAT 
GAACGTTGTA CTGCACTTTC ATCTGTGCAG ATTGTAAAGA TCCTTAACTC CTACACTCCA 
ATTGATGATT TTGAGAAACG CGTAACGCCA TCCTTTGTAC GCAAGGTCCA GTCCATGCTA 
AACAACAGAG AAGACTGCTC TCAGTTAATG CTGGATAGTA AATACCTCTT CCAAGTGACA 
TTTCCTTTCA GCCCATCTCC CCACGCACTG GAGATGATTC AAATTCCCAG CAGTCTGAAA 
CTTGGCTTCC TAACAAGAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947917.1
CDS	166..4929
Translation	MGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_018092428.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092428.1

ORF Insert Sequence:

ATGGGAGACA TAGATCCGCA TATATTTGCT GTTGCAGAAG AGGCCTACAA ACAAATGGCA 
AGGAACAATA GAAATCAGTC CATTATTGTT AGTGGAGAAT CTGGAGCAGG AAAGACAGTG 
TCAGCTCGAT ATACTATGAG GTACTTTGCC ACAGTCAGTA AAACAAGCAG CAAAGCACAC 
GTGGAAGATA AAGTGCTGGC ATCCAATCCA ATAACAGAGG CTATTGGAAA TGCAAAAACC 
ACTCGAAATG ATAACAGCAG TCGCTTTGGA AAGTATACTG AAATCAGTTT TGATAGAAGG 
TATAAAATTA TTGGTGCAAA CTTGAGAACG TATCTTCTGG AAAAATCCCG GGTAGTCTTT 
CAGTCAGAAA ATGAAAGGAA TTACCACATA TTTTACCAGC TTTGTGCTTC TGCCAACAAA 
CCGGAATTTG AACACCTTCA TCTAACGAGA GCAGAAGAGT TCAACTACAC TTCAATGGGT 
GGCTGCACAG TTATAACGGG AGTTGATGAC AAAAAAGATA TGTTGGATAC ACAAAAGACA 
TTTGCTTTAT TAGGTTTAGG CACAGACTTT CAGATGGATG TATTTAAAGT CGTAGCAGCT 
ATCCTGCACT TAGGAAATGT GGAGATCAAG CGTGTCAGTG ATGATAGATC CTCTGTTGAT 
GTAAATGACA AACACTTGAA GATATTTTGT GATCTTCTGA ATCTAGAAGC CTCAAAGGTG 
GCACAGTGGC TGTGCAACCG GAGAATTGTG ACTGTATCAG ACACTGTTAT AAAACCCATG 
ACCAAACAGC AAGCTGACAA TGCAAGGGAT GCTTTGGCCA AGAAAATCTA CTCTCACCTT 
TTTGATTATG TCATCGATAA AATAAATAAT GCATTACAGT ACCCAGGGAA ACAACATACA 
TTTATAGGTG TCCTTGATAT TTATGGGTTC GAAACATTTG AAATTAACAG TTTTGAACAG 
TTTTGCATCA ATTATGCCAA TGAGAAGCTG CAGCAACAGT TCAACCTTCA TGTTTTCAAG 
CTTGAGCAGG AAGAATATAT GAAGGAAGAC ATCCCATGGA CTCTTATTGA CTTTTATGAT 
AACCAAATAG TAATTGACCT CATTGAAGCA AAGATGGGCA TTTTGGACCT GCTAGATGAA 
GAGTGCCTGC TGCCGCAAGG AACAGACGAA AACTGGTTAC AGAAGCTGTA TAATAACTAC 
CTTAACAAGA ACCCCTTGTT TGAAAAGCCC CGAATGTCCA ACAGATCATT CATTATACAG 
CACTTTGCAG ATAAGGTAGA ATATAAGTGT GATGGATTTT TGGAGAAAAA CCGAGACACT 
GTGTATGATG TTTTAATTGA TCTTCTGAGG ACTAGTAAGT TTCAGCTGTG CTCCAGCTTT 
TTTCAAGACA ACACAAGCAA GTCAGCACAA TTCAGTTCTG GAATCCAGGT GAAATCTGCA 
AGACCTGCGA TAAAATCATC AAATAAAGAG TTCAGAATGA CTGTGGGAAG CAAGTTTCGG 
AACTCGTTAT GTTTGCTCAT GGAGACCCTT AATGCAACAA CACCACACTA TGTACGATGC 
ATAAAGCCCA ACGATTTAAA ACTTCCATTT GAGTTTGATG CCAAAAGAGT AGTTCAACAG 
CTACGAGCCT GTGGAGTTCT AGAAACCATA AGAATCAGTG CACAAAGCTA CCCTTCCAGG 
TGGACATACA TTGAGTTCTT TGGCCGTTAC AGCATTCTTA TGACTCAGCA GGAACTGTCC 
CTGAATGATA AGAAATACAT TTGCAAAATA GTACTGCAAA GACTGATCCA GGATTCCAAC 
CAATATCAGT TTGGAAGAAC CAAAATCTTC TTCAGAGCTG GCCAGGTGGC TTACTTAGAA 
AAATTGAGAT CAGACAAGCT AAGGAATGCC TGCGTCATAA TCCAAAAGAG CGTACGAGGA 
TGGGTGCAAA AGAAGAAGTT TAGCCGGGCC CGGCGTTCGG CCATTGTCAT ACAGCAGTAT 
TTCCGGGGCC AGAGAGCTGT TAGGCAAGCA ATAAGTGGAA AAGCCCTGAA GCATGCTTGG 
GCAGCAATCA TTATCCAGAA ATATGTTCGG GGATTCTTAG TCCGTCGCAT CTACCAACTT 
ATCCTGGTGG CCACAGTGAC CATTCAGTCA TTCACACGTG GTTACTTGGC TAGGAAGAGA 
TATCACAAGA TGCGAGAAGA GCACAAGGCT TTAATACTGC AGAAATACGC CCGTGCTTGG 
CTGGCAAGGC GAAGGTTCCA GAACGTCCGT CGTTTTGTGC TCAACATCCA GCTTTCCTAC 
AGAGTGCAGC GCCTGCAGAA AAAACTTGAA GATCAGAACA AAGAGAACCA TGGGCTGTTG 
GAACGTCTGA CAAGCCTTGC GTCGTCACAC ACACACGATG TGGATAAAGT GCACAAACTC 
GAATCGGAGC TGGAAAAAAT TTCCTCCCAA AAGAAACATC TGGATGAAAA GGGAAAGAAG 
TACAAGGAAG AAGCTGAGGA GAAAATGACA AAGCTTCAAG CCCAAAATGC TGAACTTCTT 
GAGCAGAAAC ATCAAATGGA AGTAAAACTC GAGACCAAAA CCCAAGAAAT GAAAGATCAG 
ATGGATCAGC TCACTAACCA ACTATTCACT GATGTGGAGA AAGAAGAAAA ACAAAGGATG 
AAACTTGAGA AACATTTTGA AGCCCAGGTT ACTGAATTCG ACACTATGAT AGAAAAATTA 
AAAGATGAAA TCCAAGTGTT AAAGGAGAGG AATAAGCATC TCCTGAAGCA GGCAGAAGAA 
GAGCAACTTG TCAATGATGG TTTAAGAGAA GAGATAGACC AACTTTCCAT CCAAGTAAAG 
AAAATACCAA CATTTCAAAC AGATATTGAG CTTCTTGAAA ACCAGAAAAG GGATTTAGAA 
CATCAACTAC AGGAACAAAA GCGTGAAATG AGAGAAAAGT TGGATGAAAT GGCAAAACAG 
CTTCTGAACA GTTTTGAAAT AGAGGATGTT AAAGCTAGAC TGGCTGAAGA GGAACTAGGA 
AATCTAAATG AAGATGGGGA AATATGGTTT GCTTATGAAG GTCTGAAAAA AGCAACCAGA 
ATACTAGAAG CTCATTTTCA GACACAGAGA GAGAACTATG AAAAGGATAT TGAGTTGTTG 
AACTTTAAGG TCGAGCACCT CCATGATGAC ATTAAACAGA TACAGAATTC TTACATGGAG 
GAAAAAGACT CAAAGGAGAG CATCACTCTT GAAGTTGAAA GGCTGAGATC TGATAATAAG 
GTGGTTAAGG ATCTGAGGAA AAAGTTGGCT GAACTTGAGG AACAAAACAT GGACCTACAG 
GGCCGTCTCT ATGAGCAAAC TATGAAATTG CCTGAAAAAT CTAACCGCAG GAAAAATTAT 
CCTGATGAGG ATGAAGTATC CCTCAAACTG TGGAATGGTC AGAGAGAAAA TGATATGGCA 
AATGTCAAAA TACAAGCAAT TCAGACAGAC AATATGTATA TGAAGGAACA AAATGAAGCA 
GACACCAGGG ACTTTGAAGA ACAGGTAGAT ATGAAAGACC GAACAATCAG GAAACTTCAA 
GATCAGATAA AGGCTCTTAC GAAGTCCATT GAAAAGGATG ATGAAGTAAG GGTGCCAACA 
GTGTTTAAAG ATTATTTGGG AATGCTGGAG TTTGCTTCAG AAGATGAATC AAAACTTATC 
AGGAATCTTA TTCTAGACCT CAAACCTCGT GGTGTTGTAG TCAACATGAT CCCAGGCCTG 
CCAGCTCACA TTATGTTCAT GTGTGTGAGG TACACAGACT ACATAAACAA TGCTGAAAGA 
ATAAAATCTC TGATGAATGC TTCCATTAAC AGCATCAAAC AGGTTATTAA GGAACATGCA 
GATGATTTTG AGATGTTGTC ATTCTGGCTT TCCAATGTCC ACCATTTTCT AAACTGCTTA 
AAGCAGTACA GCGGGGAAGA GGAATTTATG AAATATAACC AGCCCCTTCA GAATAAGAAC 
TGCTTAAAGA ATTTTGATCT GTCAGAATAT AGACAAATTA TCAGCGATTT GGCAATCCGG 
ATATATCATC AGTTCATTAC TGTAATGGAG AACAACCTTC AGCCAATGAT AGTGCCTGGC 
ATGCTGGAAT ATGAAAGCCT TCATGGAATT TCAGTCTCCA AACCAACAGG ATTCCGTAAA 
CGTTCATCTA GTATAGATGA CACAGACTCT TACACTATGA CCTCAATTCT ACAGCAGCTT 
AGTTATTTTT ATACGACTAT GTGCCAGCAT GGCATGGACC CTGAGCTCCT GAAACAAACA 
ATGAAGCAGC TTTTTTTCCT GATTGCCACT GTCACCATCA ACAGTCTTTT CCTTCGCAAG 
GACATGTGCT CGTGTAGAAA AGGAATGCAG ATTCGGTGTA ATATCAGTTA CCTTGAAGAA 
TGGCTGCAAG GCAAGAATCT TCAGGGGGCT ACAGCCAAAG AAACATTGGA GCCCCTTTCC 
CAGGCAGCAT GGCTATTACA AGTAAAAAAG ATTACAGATG ATGACGCCAA AGAAATCTAT 
GAACGTTGTA CTGCACTTTC ATCTGTGCAG ATTGTAAAGA TCCTTAACTC CTACACTCCA 
ATTGATGATT TTGAGAAACG CGTAACGCCA TCCTTTGTAC GCAAGGTCCA GTCCATGCTA 
AACAACAGAG AAGACTGCTC TCAGTTAATG CTGGATAGTA AATACCTCTT CCAAGTGACA 
TTTCCTTTCA GCCCATCTCC CCACGCACTG GAGATGATTC AAATTCCCAG CAGTCTGAAA 
CTTGGCTTCC TAACAAGAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5c ( XM_031899192.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755052.1 Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.

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CloneID	OXa55103
Clone ID Related Accession (Same CDS sequence)	XM_031899192.1
Accession Version	XM_031899192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCTG AAAGGCAAAC AAAACCAGAA AATTTCAACA GGGTCTGGAT CCCCGACGCA 
GAGGAAGTGT GGCGTTCTGC TGAAATTACA AAAGATCTGA AGATCGGGGA CACTGTGCTC 
CATTTGTGTT TGGAAGATGG AACTCACTTG AAGTATCCAG TGGAACCTGA AAAGCAGGTT 
CTCCCCCCTC TAAGAAATCC AGATATTCTT GTTGGTGAAA ATGACCTCAC AGCCCTCAGT 
TATTTGCACG AGCCAGCTGT TCTGCACAAT CTCAAAGTTC GATTTGTGGA GTCCAACGTT 
ATTTATACAT ACAGTGGTAT TATCTTGGTT GCAATCAATC CATACAAGGA GCTCCCAATC 
TATGGAGATG CAATTATCCA TGCATACAGT GGGCAAAATA TGGGAGACAT AGATCCGCAT 
ATATTTGCTG TTGCAGAAGA GGCCTACAAA CAAATGGCAA GGAACAATAG AAATCAGTCC 
ATTATTGTTA GTGGAGAATC TGGAGCAGGA AAGACAGTGT CAGCTCGATA TACTATGAGG 
TACTTTGCCA CAGTCAGTAA AACAAGCAGC AAAGCACACG TGGAAGATAA AGTGCTGGCA 
TCCAATCCAA TAACAGAGGC TATTGGAAAT GCAAAAACCA CTCGAAATGA TAACAGCAGT 
CGCTTTGGAA AGTATACTGA AATCAGTTTT GATAGAAGGT ATAAAATTAT TGGTGCAAAC 
TTGAGAACGT ATCTTCTGGA AAAATCCCGG GTAGTCTTTC AGTCAGAAAA TGAAAGGAAT 
TACCACATAT TTTACCAGCT TTGTGCTTCT GCCAACAAAC CGGAATTTGA ACACCTTCAT 
CTAACGAGAG CAGAAGAGTT CAACTACACT TCAATGGGTG GCTGCACAGT TATAACGGGA 
GTTGATGACA AAAAAGATAT GTTGGATACA CAAAAGACAT TTGCTTTATT AGGTTTAGGC 
ACAGACTTTC AGATGGATGT ATTTAAAGTC GTAGCAGCTA TCCTGCACTT AGGAAATGTG 
GAGATCAAGC GTGTCAGTGA TGATAGATCC TCTGTTGATG TAAATGACAA ACACTTGAAG 
ATATTTTGTG ATCTTCTGAA TCTAGAAGCC TCAAAGGTGG CACAGTGGCT GTGCAACCGG 
AGAATTGTGA CTGTATCAGA CACTGTTATA AAACCCATGA CCAAACAGCA AGCTGACAAT 
GCAAGGGATG CTTTGGCCAA GAAAATCTAC TCTCACCTTT TTGATTATGT CATCGATAAA 
ATAAATAATG CATTACAGTA CCCAGGGAAA CAACATACAT TTATAGGTGT CCTTGATATT 
TATGGGTTCG AAACATTTGA AATTAACAGT TTTGAACAGT TTTGCATCAA TTATGCCAAT 
GAGAAGCTGC AGCAACAGTT CAACCTTCAT GTTTTCAAGC TTGAGCAGGA AGAATATATG 
AAGGAAGACA TCCCATGGAC TCTTATTGAC TTTTATGATA ACCAAATAGT AATTGACCTC 
ATTGAAGCAA AGATGGGCAT TTTGGACCTG CTAGATGAAG AGTGCCTGCT GCCGCAAGGA 
ACAGACGAAA ACTGGTTACA GAAGCTGTAT AATAACTACC TTAACAAGAA CCCCTTGTTT 
GAAAAGCCCC GAATGTCCAA CAGATCATTC ATTATACAGC ACTTTGCAGA TAAGGTAGAA 
TATAAGTGTG ATGGATTTTT GGAGAAAAAC CGAGACACTG TGTATGATGT TTTAATTGAT 
CTTCTGAGGA CTAGTAAGTT TCAGCTGTGC TCCAGCTTTT TTCAAGACAA CACAAGCAAG 
TCAGCACAAT TCAGTTCTGG AATCCAGGTG AAATCTGCAA GACCTGCGAT AAAATCATCA 
AATAAAGAGT TCAGAATGAC TGTGGGAAGC AAGTTTCGGA ACTCGTTATG TTTGCTCATG 
GAGACCCTTA ATGCAACAAC ACCACACTAT GTACGATGCA TAAAGCCCAA CGATTTAAAA 
CTTCCATTTG AGTTTGATGC CAAAAGAGTA GTTCAACAGC TACGAGCCTG TGGAGTTCTA 
GAAACCATAA GAATCAGTGC ACAAAGCTAC CCTTCCAGGT GGACATACAT TGAGTTCTTT 
GGCCGTTACA GCATTCTTAT GACTCAGCAG GAACTGTCCC TGAATGATAA GAAATACATT 
TGCAAAATAG TACTGCAAAG ACTGATCCAG GATTCCAACC AATATCAGTT TGGAAGAACC 
AAAATCTTCT TCAGAGCTGG CCAGGTGGCT TACTTAGAAA AATTGAGATC AGACAAGCTA 
AGGAATGCCT GCGTCATAAT CCAAAAGAGC GTACGAGGAT GGGTGCAAAA GAAGAAGTTT 
AGCCGGGCCC GGCGTTCGGC CATTGTCATA CAGCAGTATT TCCGGGGCCA GAGAGCTGTT 
AGGCAAGCAA TAAGTGGAAA AGCCCTGAAG CATGCTTGGG CAGCAATCAT TATCCAGAAA 
TATGTTCGGG GATTCTTAGT CCGTCGCATC TACCAACTTA TCCTGGTGGC CACAGTGACC 
ATTCAGTCAT TCACACGTGG TTACTTGGCT AGGAAGAGAT ATCACAAGAT GCGAGAAGAG 
CACAAGGCTT TAATACTGCA GAAATACGCC CGTGCTTGGC TGGCAAGGCG AAGGTTCCAG 
AACGTCCGTC GTTTTGTGCT CAACATCCAG CTTTCCTACA GAGTGCAGCG CCTGCAGAAA 
AAACTTGAAG ATCAGAACAA AGAGAACCAT GGGCTGTTGG AACGTCTGAC AAGCCTTGCG 
TCGTCACACA CACACGATGT GGATAAAGTG CACAAACTCG AATCGGAGCT GGAAAAAATT 
TCCTCCCAAA AGAAACATCT GGATGAAAAG GGAAAGAAGT ACAAGGAAGA AGCTGAGGAG 
AAAATGACAA AGCTTCAAGC CCAAAATGCT GAACTTCTTG AGCAGAAACA TCAAATGGAA 
GTAAAACTCG AGACCAAAAC CCAAGAAATG AAAGATCAGA TGGATCAGCT CACTAACCAA 
CTATTCACTG ATGTGGAGAA AGAAGAAAAA CAAAGGATGA AACTTGAGAA ACATTTTGAA 
GCCCAGGTTA CTGAATTCGA CACTATGATA GAAAAATTAA AAGATGAAAT CCAAGTGTTA 
AAGGAGAGGA ATAAGCATCT CCTGAAGCAG GCAGAAGAAG AGCAACTTGT CAATGATGGT 
TTAAGAGAAG AGATAGACCA ACTTTCCATC CAAGTAAAGA AAATACCAAC ATTTCAAACA 
GATATTGAGC TTCTTGAAAA CCAGAAAAGG GATTTAGAAC ATCAACTACA GGAACAAAAG 
CGTGAAATGA GAGAAAAGTT GGATGAAATG GCAAAACAGC TTCTGAACAG TTTTGAAATA 
GAGGATGTTA AAGCTAGACT GGCTGAAGAG GAACTAGGAA ATCTAAATGA AGATGGGGAA 
ATATGGTTTG CTTATGAAGG TCTGAAAAAA GCAACCAGAA TACTAGAAGC TCATTTTCAG 
ACACAGAGAG AGAACTATGA AAAGGATATT GAGTTGTTGA ACTTTAAGGT CGAGCACCTC 
CATGATGACA TTAAACAGAT ACAGAATTCT TACATGGAGG AAAAAGACTC AAAGGAGAGC 
ATCACTCTTG AAGTTGAAAG GCTGAGATCT GATAATAAGG TGGTTAAGGA TCTGAGGAAA 
AAGTTGGCTG AACTTGAGGA ACAAAACATG GACCTACAGG GCCGTCTCTA TGAGCAAACT 
ATGAAATTGC CTGAAAAATC TAACCGCAGG AAAAATTATC CTGATGAGGA TGAAGTATCC 
CTCAAACTGT GGAATGGTCA GAGAGAAAAT GATATGGCAA ATGTCAAAAT ACAAGCAATT 
CAGACAGACA ATATGTATAT GAAGGAACAA AATGAAGCAG ACACCAGGGA CTTTGAAGAA 
CAGGTAGATA TGAAAGACCG AACAATCAGG AAACTTCAAG ATCAGATAAA GGCTCTTACG 
AAGTCCATTG AAAAGGATGA TGAAGTAAGG GTGCCAACAG TGTTTAAAGA TTATTTGGGA 
ATGCTGGAGT TTGCTTCAGA AGATGAATCA AAACTTATCA GGAATCTTAT TCTAGACCTC 
AAACCTCGTG GTGTTGTAGT CAACATGATC CCAGGCCTGC CAGCTCACAT TATGTTCATG 
TGTGTGAGGT ACACAGACTA CATAAACAAT GCTGAAAGAA TAAAATCTCT GATGAATGCT 
TCCATTAACA GCATCAAACA GGTTATTAAG GAACATGCAG ATGATTTTGA GATGTTGTCA 
TTCTGGCTTT CCAATGTCCA CCATTTTCTA AACTGCTTAA AGCAGTACAG CGGGGAAGAG 
GAATTTATGA AATATAACCA GCCCCTTCAG AATAAGAACT GCTTAAAGAA TTTTGATCTG 
TCAGAATATA GACAAATTAT CAGCGATTTG GCAATCCGGA TATATCATCA GTTCATTACT 
GTAATGGAGA ACAACCTTCA GCCAATGATA GTGCCTGGCA TGCTGGAATA TGAAAGCCTT 
CATGGAATTT CAGTCTCCAA ACCAACAGGA TTCCGTAAAC GTTCATCTAG TATAGATGAC 
ACAGACTCTT ACACTATGAC CTCAATTCTA CAGCAGCTTA GTTATTTTTA TACGACTATG 
TGCCAGCATG GCATGGACCC TGAGCTCCTG AAACAAACAA TGAAGCAGCT TTTTTTCCTG 
ATTGCCACTG TCACCATCAA CAGTCTTTTC CTTCGCAAGG ACATGTGCTC GTGTAGAAAA 
GGAATGCAGA TTCGGTGTAA TATCAGTTAC CTTGAAGAAT GGCTGCAAGG CAAGAATCTT 
CAGGGGGCTA CAGCCAAAGA AACATTGGAG CCCCTTTCCC AGGCAGCATG GCTATTACAA 
GTAAAAAAGA TTACAGATGA TGACGCCAAA GAAATCTATG AACGTTGTAC TGCACTTTCA 
TCTGTGCAGA TTGTAAAGAT CCTTAACTCC TACACTCCAA TTGATGATTT TGAGAAACGC 
GTAACGCCAT CCTTTGTACG CAAGGTCCAG TCCATGCTAA ACAACAGAGA AGACTGCTCT 
CAGTTAATGC TGGATAGTAA ATACCTCTTC CAAGTGACAT TTCCTTTCAG CCCATCTCCC 
CACGCACTGG AGATGATTCA AATTCCCAGC AGTCTGAAAC TTGGCTTCCT AACAAGAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755052.1
CDS	121..5283
Translation	MESERQTKPENFNRVWIPDAEEVWRSAEITKDLKIGDTVLHLCLEDGTHLKYPVEPEKQVLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESNVIYTYSGIILVAINPYKELPIYGDAIIHAYSGQNMGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_031899192.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899192.1

ORF Insert Sequence:

ATGGAATCTG AAAGGCAAAC AAAACCAGAA AATTTCAACA GGGTCTGGAT CCCCGACGCA 
GAGGAAGTGT GGCGTTCTGC TGAAATTACA AAAGATCTGA AGATCGGGGA CACTGTGCTC 
CATTTGTGTT TGGAAGATGG AACTCACTTG AAGTATCCAG TGGAACCTGA AAAGCAGGTT 
CTCCCCCCTC TAAGAAATCC AGATATTCTT GTTGGTGAAA ATGACCTCAC AGCCCTCAGT 
TATTTGCACG AGCCAGCTGT TCTGCACAAT CTCAAAGTTC GATTTGTGGA GTCCAACGTT 
ATTTATACAT ACAGTGGTAT TATCTTGGTT GCAATCAATC CATACAAGGA GCTCCCAATC 
TATGGAGATG CAATTATCCA TGCATACAGT GGGCAAAATA TGGGAGACAT AGATCCGCAT 
ATATTTGCTG TTGCAGAAGA GGCCTACAAA CAAATGGCAA GGAACAATAG AAATCAGTCC 
ATTATTGTTA GTGGAGAATC TGGAGCAGGA AAGACAGTGT CAGCTCGATA TACTATGAGG 
TACTTTGCCA CAGTCAGTAA AACAAGCAGC AAAGCACACG TGGAAGATAA AGTGCTGGCA 
TCCAATCCAA TAACAGAGGC TATTGGAAAT GCAAAAACCA CTCGAAATGA TAACAGCAGT 
CGCTTTGGAA AGTATACTGA AATCAGTTTT GATAGAAGGT ATAAAATTAT TGGTGCAAAC 
TTGAGAACGT ATCTTCTGGA AAAATCCCGG GTAGTCTTTC AGTCAGAAAA TGAAAGGAAT 
TACCACATAT TTTACCAGCT TTGTGCTTCT GCCAACAAAC CGGAATTTGA ACACCTTCAT 
CTAACGAGAG CAGAAGAGTT CAACTACACT TCAATGGGTG GCTGCACAGT TATAACGGGA 
GTTGATGACA AAAAAGATAT GTTGGATACA CAAAAGACAT TTGCTTTATT AGGTTTAGGC 
ACAGACTTTC AGATGGATGT ATTTAAAGTC GTAGCAGCTA TCCTGCACTT AGGAAATGTG 
GAGATCAAGC GTGTCAGTGA TGATAGATCC TCTGTTGATG TAAATGACAA ACACTTGAAG 
ATATTTTGTG ATCTTCTGAA TCTAGAAGCC TCAAAGGTGG CACAGTGGCT GTGCAACCGG 
AGAATTGTGA CTGTATCAGA CACTGTTATA AAACCCATGA CCAAACAGCA AGCTGACAAT 
GCAAGGGATG CTTTGGCCAA GAAAATCTAC TCTCACCTTT TTGATTATGT CATCGATAAA 
ATAAATAATG CATTACAGTA CCCAGGGAAA CAACATACAT TTATAGGTGT CCTTGATATT 
TATGGGTTCG AAACATTTGA AATTAACAGT TTTGAACAGT TTTGCATCAA TTATGCCAAT 
GAGAAGCTGC AGCAACAGTT CAACCTTCAT GTTTTCAAGC TTGAGCAGGA AGAATATATG 
AAGGAAGACA TCCCATGGAC TCTTATTGAC TTTTATGATA ACCAAATAGT AATTGACCTC 
ATTGAAGCAA AGATGGGCAT TTTGGACCTG CTAGATGAAG AGTGCCTGCT GCCGCAAGGA 
ACAGACGAAA ACTGGTTACA GAAGCTGTAT AATAACTACC TTAACAAGAA CCCCTTGTTT 
GAAAAGCCCC GAATGTCCAA CAGATCATTC ATTATACAGC ACTTTGCAGA TAAGGTAGAA 
TATAAGTGTG ATGGATTTTT GGAGAAAAAC CGAGACACTG TGTATGATGT TTTAATTGAT 
CTTCTGAGGA CTAGTAAGTT TCAGCTGTGC TCCAGCTTTT TTCAAGACAA CACAAGCAAG 
TCAGCACAAT TCAGTTCTGG AATCCAGGTG AAATCTGCAA GACCTGCGAT AAAATCATCA 
AATAAAGAGT TCAGAATGAC TGTGGGAAGC AAGTTTCGGA ACTCGTTATG TTTGCTCATG 
GAGACCCTTA ATGCAACAAC ACCACACTAT GTACGATGCA TAAAGCCCAA CGATTTAAAA 
CTTCCATTTG AGTTTGATGC CAAAAGAGTA GTTCAACAGC TACGAGCCTG TGGAGTTCTA 
GAAACCATAA GAATCAGTGC ACAAAGCTAC CCTTCCAGGT GGACATACAT TGAGTTCTTT 
GGCCGTTACA GCATTCTTAT GACTCAGCAG GAACTGTCCC TGAATGATAA GAAATACATT 
TGCAAAATAG TACTGCAAAG ACTGATCCAG GATTCCAACC AATATCAGTT TGGAAGAACC 
AAAATCTTCT TCAGAGCTGG CCAGGTGGCT TACTTAGAAA AATTGAGATC AGACAAGCTA 
AGGAATGCCT GCGTCATAAT CCAAAAGAGC GTACGAGGAT GGGTGCAAAA GAAGAAGTTT 
AGCCGGGCCC GGCGTTCGGC CATTGTCATA CAGCAGTATT TCCGGGGCCA GAGAGCTGTT 
AGGCAAGCAA TAAGTGGAAA AGCCCTGAAG CATGCTTGGG CAGCAATCAT TATCCAGAAA 
TATGTTCGGG GATTCTTAGT CCGTCGCATC TACCAACTTA TCCTGGTGGC CACAGTGACC 
ATTCAGTCAT TCACACGTGG TTACTTGGCT AGGAAGAGAT ATCACAAGAT GCGAGAAGAG 
CACAAGGCTT TAATACTGCA GAAATACGCC CGTGCTTGGC TGGCAAGGCG AAGGTTCCAG 
AACGTCCGTC GTTTTGTGCT CAACATCCAG CTTTCCTACA GAGTGCAGCG CCTGCAGAAA 
AAACTTGAAG ATCAGAACAA AGAGAACCAT GGGCTGTTGG AACGTCTGAC AAGCCTTGCG 
TCGTCACACA CACACGATGT GGATAAAGTG CACAAACTCG AATCGGAGCT GGAAAAAATT 
TCCTCCCAAA AGAAACATCT GGATGAAAAG GGAAAGAAGT ACAAGGAAGA AGCTGAGGAG 
AAAATGACAA AGCTTCAAGC CCAAAATGCT GAACTTCTTG AGCAGAAACA TCAAATGGAA 
GTAAAACTCG AGACCAAAAC CCAAGAAATG AAAGATCAGA TGGATCAGCT CACTAACCAA 
CTATTCACTG ATGTGGAGAA AGAAGAAAAA CAAAGGATGA AACTTGAGAA ACATTTTGAA 
GCCCAGGTTA CTGAATTCGA CACTATGATA GAAAAATTAA AAGATGAAAT CCAAGTGTTA 
AAGGAGAGGA ATAAGCATCT CCTGAAGCAG GCAGAAGAAG AGCAACTTGT CAATGATGGT 
TTAAGAGAAG AGATAGACCA ACTTTCCATC CAAGTAAAGA AAATACCAAC ATTTCAAACA 
GATATTGAGC TTCTTGAAAA CCAGAAAAGG GATTTAGAAC ATCAACTACA GGAACAAAAG 
CGTGAAATGA GAGAAAAGTT GGATGAAATG GCAAAACAGC TTCTGAACAG TTTTGAAATA 
GAGGATGTTA AAGCTAGACT GGCTGAAGAG GAACTAGGAA ATCTAAATGA AGATGGGGAA 
ATATGGTTTG CTTATGAAGG TCTGAAAAAA GCAACCAGAA TACTAGAAGC TCATTTTCAG 
ACACAGAGAG AGAACTATGA AAAGGATATT GAGTTGTTGA ACTTTAAGGT CGAGCACCTC 
CATGATGACA TTAAACAGAT ACAGAATTCT TACATGGAGG AAAAAGACTC AAAGGAGAGC 
ATCACTCTTG AAGTTGAAAG GCTGAGATCT GATAATAAGG TGGTTAAGGA TCTGAGGAAA 
AAGTTGGCTG AACTTGAGGA ACAAAACATG GACCTACAGG GCCGTCTCTA TGAGCAAACT 
ATGAAATTGC CTGAAAAATC TAACCGCAGG AAAAATTATC CTGATGAGGA TGAAGTATCC 
CTCAAACTGT GGAATGGTCA GAGAGAAAAT GATATGGCAA ATGTCAAAAT ACAAGCAATT 
CAGACAGACA ATATGTATAT GAAGGAACAA AATGAAGCAG ACACCAGGGA CTTTGAAGAA 
CAGGTAGATA TGAAAGACCG AACAATCAGG AAACTTCAAG ATCAGATAAA GGCTCTTACG 
AAGTCCATTG AAAAGGATGA TGAAGTAAGG GTGCCAACAG TGTTTAAAGA TTATTTGGGA 
ATGCTGGAGT TTGCTTCAGA AGATGAATCA AAACTTATCA GGAATCTTAT TCTAGACCTC 
AAACCTCGTG GTGTTGTAGT CAACATGATC CCAGGCCTGC CAGCTCACAT TATGTTCATG 
TGTGTGAGGT ACACAGACTA CATAAACAAT GCTGAAAGAA TAAAATCTCT GATGAATGCT 
TCCATTAACA GCATCAAACA GGTTATTAAG GAACATGCAG ATGATTTTGA GATGTTGTCA 
TTCTGGCTTT CCAATGTCCA CCATTTTCTA AACTGCTTAA AGCAGTACAG CGGGGAAGAG 
GAATTTATGA AATATAACCA GCCCCTTCAG AATAAGAACT GCTTAAAGAA TTTTGATCTG 
TCAGAATATA GACAAATTAT CAGCGATTTG GCAATCCGGA TATATCATCA GTTCATTACT 
GTAATGGAGA ACAACCTTCA GCCAATGATA GTGCCTGGCA TGCTGGAATA TGAAAGCCTT 
CATGGAATTT CAGTCTCCAA ACCAACAGGA TTCCGTAAAC GTTCATCTAG TATAGATGAC 
ACAGACTCTT ACACTATGAC CTCAATTCTA CAGCAGCTTA GTTATTTTTA TACGACTATG 
TGCCAGCATG GCATGGACCC TGAGCTCCTG AAACAAACAA TGAAGCAGCT TTTTTTCCTG 
ATTGCCACTG TCACCATCAA CAGTCTTTTC CTTCGCAAGG ACATGTGCTC GTGTAGAAAA 
GGAATGCAGA TTCGGTGTAA TATCAGTTAC CTTGAAGAAT GGCTGCAAGG CAAGAATCTT 
CAGGGGGCTA CAGCCAAAGA AACATTGGAG CCCCTTTCCC AGGCAGCATG GCTATTACAA 
GTAAAAAAGA TTACAGATGA TGACGCCAAA GAAATCTATG AACGTTGTAC TGCACTTTCA 
TCTGTGCAGA TTGTAAAGAT CCTTAACTCC TACACTCCAA TTGATGATTT TGAGAAACGC 
GTAACGCCAT CCTTTGTACG CAAGGTCCAG TCCATGCTAA ACAACAGAGA AGACTGCTCT 
CAGTTAATGC TGGATAGTAA ATACCTCTTC CAAGTGACAT TTCCTTTCAG CCCATCTCCC 
CACGCACTGG AGATGATTCA AATTCCCAGC AGTCTGAAAC TTGGCTTCCT AACAAGAATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5c ( XM_002936346.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936392.2 Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.

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CloneID	OXa07362
Clone ID Related Accession (Same CDS sequence)	XM_002936346.4 , XM_002936346.5
Accession Version	XM_002936346.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936346.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAT TAGCTGAGCT GTACACTCAG TTCAACAGGG TCTGGATCCC CGACGCAGAG 
GAAGTGTGGC GTTCTGCTGA AATTACAAAA GATCTGAAGA TCGGGGACAC TGTGCTCCAT 
TTGTGTTTGG AAGATGGAAC TCACTTGAAG TATCCAGTGG AACCTGAAAA GCAGGTTCTC 
CCCCCTCTAA GAAATCCAGA TATTCTTGTT GGTGAAAATG ACCTCACAGC CCTCAGTTAT 
TTGCACGAGC CAGCTGTTCT GCACAATCTC AAAGTTCGAT TTGTGGAGTC CAACGTTATT 
TATACATACA GTGGTATTAT CTTGGTTGCA ATCAATCCAT ACAAGGAGCT CCCAATCTAT 
GGAGATGCAA TTATCCATGC ATACAGTGGG CAAAATATGG GAGACATAGA TCCGCATATA 
TTTGCTGTTG CAGAAGAGGC CTACAAACAA ATGGCAAGGA ACAATAGAAA TCAGTCCATT 
ATTGTTAGTG GAGAATCTGG AGCAGGAAAG ACAGTGTCAG CTCGATATAC TATGAGGTAC 
TTTGCCACAG TCAGTAAAAC AAGCAGCAAA GCACACGTGG AAGATAAAGT GCTGGCATCC 
AATCCAATAA CAGAGGCTAT TGGAAATGCA AAAACCACTC GAAATGATAA CAGCAGTCGC 
TTTGGAAAGT ATACTGAAAT CAGTTTTGAT AGAAGGTATA AAATTATTGG TGCAAACTTG 
AGAACGTATC TTCTGGAAAA ATCCCGGGTA GTCTTTCAGT CAGAAAATGA AAGGAATTAC 
CACATATTTT ACCAGCTTTG TGCTTCTGCC AACAAACCGG AATTTGAACA CCTTCATCTA 
ACGAGAGCAG AAGAGTTCAA CTACACTTCA ATGGGTGGCT GCACAGTTAT AACGGGAGTT 
GATGACAAAA AAGATATGTT GGATACACAA AAGACATTTG CTTTATTAGG TTTAGGCACA 
GACTTTCAGA TGGATGTATT TAAAGTCGTA GCAGCTATCC TGCACTTAGG AAATGTGGAG 
ATCAAGCGTG TCAGTGATGA TAGATCCTCT GTTGATGTAA ATGACAAACA CTTGAAGATA 
TTTTGTGATC TTCTGAATCT AGAAGCCTCA AAGGTGGCAC AGTGGCTGTG CAACCGGAGA 
ATTGTGACTG TATCAGACAC TGTTATAAAA CCCATGACCA AACAGCAAGC TGACAATGCA 
AGGGATGCTT TGGCCAAGAA AATCTACTCT CACCTTTTTG ATTATGTCAT CGATAAAATA 
AATAATGCAT TACAGTACCC AGGGAAACAA CATACATTTA TAGGTGTCCT TGATATTTAT 
GGGTTCGAAA CATTTGAAAT TAACAGTTTT GAACAGTTTT GCATCAATTA TGCCAATGAG 
AAGCTGCAGC AACAGTTCAA CCTTCATGTT TTCAAGCTTG AGCAGGAAGA ATATATGAAG 
GAAGACATCC CATGGACTCT TATTGACTTT TATGATAACC AAATAGTAAT TGACCTCATT 
GAAGCAAAGA TGGGCATTTT GGACCTGCTA GATGAAGAGT GCCTGCTGCC GCAAGGAACA 
GACGAAAACT GGTTACAGAA GCTGTATAAT AACTACCTTA ACAAGAACCC CTTGTTTGAA 
AAGCCCCGAA TGTCCAACAG ATCATTCATT ATACAGCACT TTGCAGATAA GGTAGAATAT 
AAGTGTGATG GATTTTTGGA GAAAAACCGA GACACTGTGT ATGATGTTTT AATTGATCTT 
CTGAGGACTA GTAAGTTTCA GCTGTGCTCC AGCTTTTTTC AAGACAACAC AAGCAAGTCA 
GCACAATTCA GTTCTGGAAT CCAGGTGAAA TCTGCAAGAC CTGCGATAAA ATCATCAAAT 
AAAGAGTTCA GAATGACTGT GGGAAGCAAG TTTCGGAACT CGTTATGTTT GCTCATGGAG 
ACCCTTAATG CAACAACACC ACACTATGTA CGATGCATAA AGCCCAACGA TTTAAAACTT 
CCATTTGAGT TTGATGCCAA AAGAGTAGTT CAACAGCTAC GAGCCTGTGG AGTTCTAGAA 
ACCATAAGAA TCAGTGCACA AAGCTACCCT TCCAGGTGGA CATACATTGA GTTCTTTGGC 
CGTTACAGCA TTCTTATGAC TCAGCAGGAA CTGTCCCTGA ATGATAAGAA ATACATTTGC 
AAAATAGTAC TGCAAAGACT GATCCAGGAT TCCAACCAAT ATCAGTTTGG AAGAACCAAA 
ATCTTCTTCA GAGCTGGCCA GGTGGCTTAC TTAGAAAAAT TGAGATCAGA CAAGCTAAGG 
AATGCCTGCG TCATAATCCA AAAGAGCGTA CGAGGATGGG TGCAAAAGAA GAAGTTTAGC 
CGGGCCCGGC GTTCGGCCAT TGTCATACAG CAGTATTTCC GGGGCCAGAG AGCTGTTAGG 
CAAGCAATAA GTGGAAAAGC CCTGAAGCAT GCTTGGGCAG CAATCATTAT CCAGAAATAT 
GTTCGGGGAT TCTTAGTCCG TCGCATCTAC CAACTTATCC TGGTGGCCAC AGTGACCATT 
CAGTCATTCA CACGTGGTTA CTTGGCTAGG AAGAGATATC ACAAGATGCG AGAAGAGCAC 
AAGGCTTTAA TACTGCAGAA ATACGCCCGT GCTTGGCTGG CAAGGCGAAG GTTCCAGAAC 
GTCCGTCGTT TTGTGCTCAA CATCCAGCTT TCCTACAGAG TGCAGCGCCT GCAGAAAAAA 
CTTGAAGATC AGAACAAAGA GAACCATGGG CTGTTGGAAC GTCTGACAAG CCTTGCGTCG 
TCACACACAC ACGATGTGGA TAAAGTGCAC AAACTCGAAT CGGAGCTGGA AAAAATTTCC 
TCCCAAAAGA AACATCTGGA TGAAAAGGGA AAGAAGTACA AGGAAGAAGC TGAGGAGAAA 
ATGACAAAGC TTCAAGCCCA AAATGCTGAA CTTCTTGAGC AGAAACATCA AATGGAAGTA 
AAACTCGAGA CCAAAACCCA AGAAATGAAA GATCAGATGG ATCAGCTCAC TAACCAACTA 
TTCACTGATG TGGAGAAAGA AGAAAAACAA AGGATGAAAC TTGAGAAACA TTTTGAAGCC 
CAGGTTACTG AATTCGACAC TATGATAGAA AAATTAAAAG ATGAAATCCA AGTGTTAAAG 
GAGAGGAATA AGCATCTCCT GAAGCAGGCA GAAGAAGAGC AACTTGTCAA TGATGGTTTA 
AGAGAAGAGA TAGACCAACT TTCCATCCAA GTAAAGAAAA TACCAACATT TCAAACAGAT 
ATTGAGCTTC TTGAAAACCA GAAAAGGGAT TTAGAACATC AACTACAGGA ACAAAAGCGT 
GAAATGAGAG AAAAGTTGGA TGAAATGGCA AAACAGCTTC TGAACAGTTT TGAAATAGAG 
GATGTTAAAG CTAGACTGGC TGAAGAGGAA CTAGGAAATC TAAATGAAGA TGGGGAAATA 
TGGTTTGCTT ATGAAGGTCT GAAAAAAGCA ACCAGAATAC TAGAAGCTCA TTTTCAGACA 
CAGAGAGAGA ACTATGAAAA GGATATTGAG TTGTTGAACT TTAAGGTCGA GCACCTCCAT 
GATGACATTA AACAGATACA GAATTCTTAC ATGGAGGAAA AAGACTCAAA GGAGAGCATC 
ACTCTTGAAG TTGAAAGGCT GAGATCTGAT AATAAGGTGG TTAAGGATCT GAGGAAAAAG 
TTGGCTGAAC TTGAGGAACA AAACATGGAC CTACAGGGCC GTCTCTATGA GCAAACTATG 
AAATTGCCTG AAAAATCTAA CCGCAGGAAA AATTATCCTG ATGAGGATGA AGTATCCCTC 
AAACTGTGGA ATGGTCAGAG AGAAAATGAT ATGGCAAATG TCAAAATACA AGCAATTCAG 
ACAGACAATA TGTATATGAA GGAACAAAAT GAAGCAGACA CCAGGGACTT TGAAGAACAG 
GTAGATATGA AAGACCGAAC AATCAGGAAA CTTCAAGATC AGATAAAGGC TCTTACGAAG 
TCCATTGAAA AGGATGATGA AGTAAGGGTG CCAACAGTGT TTAAAGATTA TTTGGGAATG 
CTGGAGTTTG CTTCAGAAGA TGAATCAAAA CTTATCAGGA ATCTTATTCT AGACCTCAAA 
CCTCGTGGTG TTGTAGTCAA CATGATCCCA GGCCTGCCAG CTCACATTAT GTTCATGTGT 
GTGAGGTACA CAGACTACAT AAACAATGCT GAAAGAATAA AATCTCTGAT GAATGCTTCC 
ATTAACAGCA TCAAACAGGT TATTAAGGAA CATGCAGATG ATTTTGAGAT GTTGTCATTC 
TGGCTTTCCA ATGTCCACCA TTTTCTAAAC TGCTTAAAGC AGTACAGCGG GGAAGAGGAA 
TTTATGAAAT ATAACCAGCC CCTTCAGAAT AAGAACTGCT TAAAGAATTT TGATCTGTCA 
GAATATAGAC AAATTATCAG CGATTTGGCA ATCCGGATAT ATCATCAGTT CATTACTGTA 
ATGGAGAACA ACCTTCAGCC AATGATAGTG CCTGGCATGC TGGAATATGA AAGCCTTCAT 
GGAATTTCAG TCTCCAAACC AACAGGATTC CGTAAACGTT CATCTAGTAT AGATGACACA 
GACTCTTACA CTATGACCTC AATTCTACAG CAGCTTAGTT ATTTTTATAC GACTATGTGC 
CAGCATGGCA TGGACCCTGA GCTCCTGAAA CAAACAATGA AGCAGCTTTT TTTCCTGATT 
GCCACTGTCA CCATCAACAG TCTTTTCCTT CGCAAGGACA TGTGCTCGTG TAGAAAAGGA 
ATGCAGATTC GGTGTAATAT CAGTTACCTT GAAGAATGGC TGCAAGGCAA GAATCTTCAG 
GGGGCTACAG CCAAAGAAAC ATTGGAGCCC CTTTCCCAGG CAGCATGGCT ATTACAAGTA 
AAAAAGATTA CAGATGATGA CGCCAAAGAA ATCTATGAAC GTTGTACTGC ACTTTCATCT 
GTGCAGATTG TAAAGATCCT TAACTCCTAC ACTCCAATTG ATGATTTTGA GAAACGCGTA 
ACGCCATCCT TTGTACGCAA GGTCCAGTCC ATGCTAAACA ACAGAGAAGA CTGCTCTCAG 
TTAATGCTGG ATAGTAAATA CCTCTTCCAA GTGACATTTC CTTTCAGCCC ATCTCCCCAC 
GCACTGGAGA TGATTCAAAT TCCCAGCAGT CTGAAACTTG GCTTCCTAAC AAGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936392.2
CDS	295..5454
Translation	MALLAELYTQFNRVWIPDAEEVWRSAEITKDLKIGDTVLHLCLEDGTHLKYPVEPEKQVLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESNVIYTYSGIILVAINPYKELPIYGDAIIHAYSGQNMGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_002936346.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936346.4

ORF Insert Sequence:

ATGGCGCTAT TAGCTGAGCT GTACACTCAG TTCAACAGGG TCTGGATCCC CGACGCAGAG 
GAAGTGTGGC GTTCTGCTGA AATTACAAAA GATCTGAAGA TCGGGGACAC TGTGCTCCAT 
TTGTGTTTGG AAGATGGAAC TCACTTGAAG TATCCAGTGG AACCTGAAAA GCAGGTTCTC 
CCCCCTCTAA GAAATCCAGA TATTCTTGTT GGTGAAAATG ACCTCACAGC CCTCAGTTAT 
TTGCACGAGC CAGCTGTTCT GCACAATCTC AAAGTTCGAT TTGTGGAGTC CAACGTTATT 
TATACATACA GTGGTATTAT CTTGGTTGCA ATCAATCCAT ACAAGGAGCT CCCAATCTAT 
GGAGATGCAA TTATCCATGC ATACAGTGGG CAAAATATGG GAGACATAGA TCCGCATATA 
TTTGCTGTTG CAGAAGAGGC CTACAAACAA ATGGCAAGGA ACAATAGAAA TCAGTCCATT 
ATTGTTAGTG GAGAATCTGG AGCAGGAAAG ACAGTGTCAG CTCGATATAC TATGAGGTAC 
TTTGCCACAG TCAGTAAAAC AAGCAGCAAA GCACACGTGG AAGATAAAGT GCTGGCATCC 
AATCCAATAA CAGAGGCTAT TGGAAATGCA AAAACCACTC GAAATGATAA CAGCAGTCGC 
TTTGGAAAGT ATACTGAAAT CAGTTTTGAT AGAAGGTATA AAATTATTGG TGCAAACTTG 
AGAACGTATC TTCTGGAAAA ATCCCGGGTA GTCTTTCAGT CAGAAAATGA AAGGAATTAC 
CACATATTTT ACCAGCTTTG TGCTTCTGCC AACAAACCGG AATTTGAACA CCTTCATCTA 
ACGAGAGCAG AAGAGTTCAA CTACACTTCA ATGGGTGGCT GCACAGTTAT AACGGGAGTT 
GATGACAAAA AAGATATGTT GGATACACAA AAGACATTTG CTTTATTAGG TTTAGGCACA 
GACTTTCAGA TGGATGTATT TAAAGTCGTA GCAGCTATCC TGCACTTAGG AAATGTGGAG 
ATCAAGCGTG TCAGTGATGA TAGATCCTCT GTTGATGTAA ATGACAAACA CTTGAAGATA 
TTTTGTGATC TTCTGAATCT AGAAGCCTCA AAGGTGGCAC AGTGGCTGTG CAACCGGAGA 
ATTGTGACTG TATCAGACAC TGTTATAAAA CCCATGACCA AACAGCAAGC TGACAATGCA 
AGGGATGCTT TGGCCAAGAA AATCTACTCT CACCTTTTTG ATTATGTCAT CGATAAAATA 
AATAATGCAT TACAGTACCC AGGGAAACAA CATACATTTA TAGGTGTCCT TGATATTTAT 
GGGTTCGAAA CATTTGAAAT TAACAGTTTT GAACAGTTTT GCATCAATTA TGCCAATGAG 
AAGCTGCAGC AACAGTTCAA CCTTCATGTT TTCAAGCTTG AGCAGGAAGA ATATATGAAG 
GAAGACATCC CATGGACTCT TATTGACTTT TATGATAACC AAATAGTAAT TGACCTCATT 
GAAGCAAAGA TGGGCATTTT GGACCTGCTA GATGAAGAGT GCCTGCTGCC GCAAGGAACA 
GACGAAAACT GGTTACAGAA GCTGTATAAT AACTACCTTA ACAAGAACCC CTTGTTTGAA 
AAGCCCCGAA TGTCCAACAG ATCATTCATT ATACAGCACT TTGCAGATAA GGTAGAATAT 
AAGTGTGATG GATTTTTGGA GAAAAACCGA GACACTGTGT ATGATGTTTT AATTGATCTT 
CTGAGGACTA GTAAGTTTCA GCTGTGCTCC AGCTTTTTTC AAGACAACAC AAGCAAGTCA 
GCACAATTCA GTTCTGGAAT CCAGGTGAAA TCTGCAAGAC CTGCGATAAA ATCATCAAAT 
AAAGAGTTCA GAATGACTGT GGGAAGCAAG TTTCGGAACT CGTTATGTTT GCTCATGGAG 
ACCCTTAATG CAACAACACC ACACTATGTA CGATGCATAA AGCCCAACGA TTTAAAACTT 
CCATTTGAGT TTGATGCCAA AAGAGTAGTT CAACAGCTAC GAGCCTGTGG AGTTCTAGAA 
ACCATAAGAA TCAGTGCACA AAGCTACCCT TCCAGGTGGA CATACATTGA GTTCTTTGGC 
CGTTACAGCA TTCTTATGAC TCAGCAGGAA CTGTCCCTGA ATGATAAGAA ATACATTTGC 
AAAATAGTAC TGCAAAGACT GATCCAGGAT TCCAACCAAT ATCAGTTTGG AAGAACCAAA 
ATCTTCTTCA GAGCTGGCCA GGTGGCTTAC TTAGAAAAAT TGAGATCAGA CAAGCTAAGG 
AATGCCTGCG TCATAATCCA AAAGAGCGTA CGAGGATGGG TGCAAAAGAA GAAGTTTAGC 
CGGGCCCGGC GTTCGGCCAT TGTCATACAG CAGTATTTCC GGGGCCAGAG AGCTGTTAGG 
CAAGCAATAA GTGGAAAAGC CCTGAAGCAT GCTTGGGCAG CAATCATTAT CCAGAAATAT 
GTTCGGGGAT TCTTAGTCCG TCGCATCTAC CAACTTATCC TGGTGGCCAC AGTGACCATT 
CAGTCATTCA CACGTGGTTA CTTGGCTAGG AAGAGATATC ACAAGATGCG AGAAGAGCAC 
AAGGCTTTAA TACTGCAGAA ATACGCCCGT GCTTGGCTGG CAAGGCGAAG GTTCCAGAAC 
GTCCGTCGTT TTGTGCTCAA CATCCAGCTT TCCTACAGAG TGCAGCGCCT GCAGAAAAAA 
CTTGAAGATC AGAACAAAGA GAACCATGGG CTGTTGGAAC GTCTGACAAG CCTTGCGTCG 
TCACACACAC ACGATGTGGA TAAAGTGCAC AAACTCGAAT CGGAGCTGGA AAAAATTTCC 
TCCCAAAAGA AACATCTGGA TGAAAAGGGA AAGAAGTACA AGGAAGAAGC TGAGGAGAAA 
ATGACAAAGC TTCAAGCCCA AAATGCTGAA CTTCTTGAGC AGAAACATCA AATGGAAGTA 
AAACTCGAGA CCAAAACCCA AGAAATGAAA GATCAGATGG ATCAGCTCAC TAACCAACTA 
TTCACTGATG TGGAGAAAGA AGAAAAACAA AGGATGAAAC TTGAGAAACA TTTTGAAGCC 
CAGGTTACTG AATTCGACAC TATGATAGAA AAATTAAAAG ATGAAATCCA AGTGTTAAAG 
GAGAGGAATA AGCATCTCCT GAAGCAGGCA GAAGAAGAGC AACTTGTCAA TGATGGTTTA 
AGAGAAGAGA TAGACCAACT TTCCATCCAA GTAAAGAAAA TACCAACATT TCAAACAGAT 
ATTGAGCTTC TTGAAAACCA GAAAAGGGAT TTAGAACATC AACTACAGGA ACAAAAGCGT 
GAAATGAGAG AAAAGTTGGA TGAAATGGCA AAACAGCTTC TGAACAGTTT TGAAATAGAG 
GATGTTAAAG CTAGACTGGC TGAAGAGGAA CTAGGAAATC TAAATGAAGA TGGGGAAATA 
TGGTTTGCTT ATGAAGGTCT GAAAAAAGCA ACCAGAATAC TAGAAGCTCA TTTTCAGACA 
CAGAGAGAGA ACTATGAAAA GGATATTGAG TTGTTGAACT TTAAGGTCGA GCACCTCCAT 
GATGACATTA AACAGATACA GAATTCTTAC ATGGAGGAAA AAGACTCAAA GGAGAGCATC 
ACTCTTGAAG TTGAAAGGCT GAGATCTGAT AATAAGGTGG TTAAGGATCT GAGGAAAAAG 
TTGGCTGAAC TTGAGGAACA AAACATGGAC CTACAGGGCC GTCTCTATGA GCAAACTATG 
AAATTGCCTG AAAAATCTAA CCGCAGGAAA AATTATCCTG ATGAGGATGA AGTATCCCTC 
AAACTGTGGA ATGGTCAGAG AGAAAATGAT ATGGCAAATG TCAAAATACA AGCAATTCAG 
ACAGACAATA TGTATATGAA GGAACAAAAT GAAGCAGACA CCAGGGACTT TGAAGAACAG 
GTAGATATGA AAGACCGAAC AATCAGGAAA CTTCAAGATC AGATAAAGGC TCTTACGAAG 
TCCATTGAAA AGGATGATGA AGTAAGGGTG CCAACAGTGT TTAAAGATTA TTTGGGAATG 
CTGGAGTTTG CTTCAGAAGA TGAATCAAAA CTTATCAGGA ATCTTATTCT AGACCTCAAA 
CCTCGTGGTG TTGTAGTCAA CATGATCCCA GGCCTGCCAG CTCACATTAT GTTCATGTGT 
GTGAGGTACA CAGACTACAT AAACAATGCT GAAAGAATAA AATCTCTGAT GAATGCTTCC 
ATTAACAGCA TCAAACAGGT TATTAAGGAA CATGCAGATG ATTTTGAGAT GTTGTCATTC 
TGGCTTTCCA ATGTCCACCA TTTTCTAAAC TGCTTAAAGC AGTACAGCGG GGAAGAGGAA 
TTTATGAAAT ATAACCAGCC CCTTCAGAAT AAGAACTGCT TAAAGAATTT TGATCTGTCA 
GAATATAGAC AAATTATCAG CGATTTGGCA ATCCGGATAT ATCATCAGTT CATTACTGTA 
ATGGAGAACA ACCTTCAGCC AATGATAGTG CCTGGCATGC TGGAATATGA AAGCCTTCAT 
GGAATTTCAG TCTCCAAACC AACAGGATTC CGTAAACGTT CATCTAGTAT AGATGACACA 
GACTCTTACA CTATGACCTC AATTCTACAG CAGCTTAGTT ATTTTTATAC GACTATGTGC 
CAGCATGGCA TGGACCCTGA GCTCCTGAAA CAAACAATGA AGCAGCTTTT TTTCCTGATT 
GCCACTGTCA CCATCAACAG TCTTTTCCTT CGCAAGGACA TGTGCTCGTG TAGAAAAGGA 
ATGCAGATTC GGTGTAATAT CAGTTACCTT GAAGAATGGC TGCAAGGCAA GAATCTTCAG 
GGGGCTACAG CCAAAGAAAC ATTGGAGCCC CTTTCCCAGG CAGCATGGCT ATTACAAGTA 
AAAAAGATTA CAGATGATGA CGCCAAAGAA ATCTATGAAC GTTGTACTGC ACTTTCATCT 
GTGCAGATTG TAAAGATCCT TAACTCCTAC ACTCCAATTG ATGATTTTGA GAAACGCGTA 
ACGCCATCCT TTGTACGCAA GGTCCAGTCC ATGCTAAACA ACAGAGAAGA CTGCTCTCAG 
TTAATGCTGG ATAGTAAATA CCTCTTCCAA GTGACATTTC CTTTCAGCCC ATCTCCCCAC 
GCACTGGAGA TGATTCAAAT TCCCAGCAGT CTGAAACTTG GCTTCCTAAC AAGAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5c ( XM_012959596.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815050.1 Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.

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CloneID	OXa36221
Clone ID Related Accession (Same CDS sequence)	XM_012959596.2 , XM_012959596.3
Accession Version	XM_012959596.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vc isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACATA GTGACCCCCA GCACAGCAAG CAAAAGTTCA ACAGGGTCTG GATCCCCGAC 
GCAGAGGAAG TGTGGCGTTC TGCTGAAATT ACAAAAGATC TGAAGATCGG GGACACTGTG 
CTCCATTTGT GTTTGGAAGA TGGAACTCAC TTGAAGTATC CAGTGGAACC TGAAAAGCAG 
GTTCTCCCCC CTCTAAGAAA TCCAGATATT CTTGTTGGTG AAAATGACCT CACAGCCCTC 
AGTTATTTGC ACGAGCCAGC TGTTCTGCAC AATCTCAAAG TTCGATTTGT GGAGTCCAAC 
GTTATTTATA CATACAGTGG TATTATCTTG GTTGCAATCA ATCCATACAA GGAGCTCCCA 
ATCTATGGAG ATGCAATTAT CCATGCATAC AGTGGGCAAA ATATGGGAGA CATAGATCCG 
CATATATTTG CTGTTGCAGA AGAGGCCTAC AAACAAATGG CAAGGAACAA TAGAAATCAG 
TCCATTATTG TTAGTGGAGA ATCTGGAGCA GGAAAGACAG TGTCAGCTCG ATATACTATG 
AGGTACTTTG CCACAGTCAG TAAAACAAGC AGCAAAGCAC ACGTGGAAGA TAAAGTGCTG 
GCATCCAATC CAATAACAGA GGCTATTGGA AATGCAAAAA CCACTCGAAA TGATAACAGC 
AGTCGCTTTG GAAAGTATAC TGAAATCAGT TTTGATAGAA GGTATAAAAT TATTGGTGCA 
AACTTGAGAA CGTATCTTCT GGAAAAATCC CGGGTAGTCT TTCAGTCAGA AAATGAAAGG 
AATTACCACA TATTTTACCA GCTTTGTGCT TCTGCCAACA AACCGGAATT TGAACACCTT 
CATCTAACGA GAGCAGAAGA GTTCAACTAC ACTTCAATGG GTGGCTGCAC AGTTATAACG 
GGAGTTGATG ACAAAAAAGA TATGTTGGAT ACACAAAAGA CATTTGCTTT ATTAGGTTTA 
GGCACAGACT TTCAGATGGA TGTATTTAAA GTCGTAGCAG CTATCCTGCA CTTAGGAAAT 
GTGGAGATCA AGCGTGTCAG TGATGATAGA TCCTCTGTTG ATGTAAATGA CAAACACTTG 
AAGATATTTT GTGATCTTCT GAATCTAGAA GCCTCAAAGG TGGCACAGTG GCTGTGCAAC 
CGGAGAATTG TGACTGTATC AGACACTGTT ATAAAACCCA TGACCAAACA GCAAGCTGAC 
AATGCAAGGG ATGCTTTGGC CAAGAAAATC TACTCTCACC TTTTTGATTA TGTCATCGAT 
AAAATAAATA ATGCATTACA GTACCCAGGG AAACAACATA CATTTATAGG TGTCCTTGAT 
ATTTATGGGT TCGAAACATT TGAAATTAAC AGTTTTGAAC AGTTTTGCAT CAATTATGCC 
AATGAGAAGC TGCAGCAACA GTTCAACCTT CATGTTTTCA AGCTTGAGCA GGAAGAATAT 
ATGAAGGAAG ACATCCCATG GACTCTTATT GACTTTTATG ATAACCAAAT AGTAATTGAC 
CTCATTGAAG CAAAGATGGG CATTTTGGAC CTGCTAGATG AAGAGTGCCT GCTGCCGCAA 
GGAACAGACG AAAACTGGTT ACAGAAGCTG TATAATAACT ACCTTAACAA GAACCCCTTG 
TTTGAAAAGC CCCGAATGTC CAACAGATCA TTCATTATAC AGCACTTTGC AGATAAGGTA 
GAATATAAGT GTGATGGATT TTTGGAGAAA AACCGAGACA CTGTGTATGA TGTTTTAATT 
GATCTTCTGA GGACTAGTAA GTTTCAGCTG TGCTCCAGCT TTTTTCAAGA CAACACAAGC 
AAGTCAGCAC AATTCAGTTC TGGAATCCAG GTGAAATCTG CAAGACCTGC GATAAAATCA 
TCAAATAAAG AGTTCAGAAT GACTGTGGGA AGCAAGTTTC GGAACTCGTT ATGTTTGCTC 
ATGGAGACCC TTAATGCAAC AACACCACAC TATGTACGAT GCATAAAGCC CAACGATTTA 
AAACTTCCAT TTGAGTTTGA TGCCAAAAGA GTAGTTCAAC AGCTACGAGC CTGTGGAGTT 
CTAGAAACCA TAAGAATCAG TGCACAAAGC TACCCTTCCA GGTGGACATA CATTGAGTTC 
TTTGGCCGTT ACAGCATTCT TATGACTCAG CAGGAACTGT CCCTGAATGA TAAGAAATAC 
ATTTGCAAAA TAGTACTGCA AAGACTGATC CAGGATTCCA ACCAATATCA GTTTGGAAGA 
ACCAAAATCT TCTTCAGAGC TGGCCAGGTG GCTTACTTAG AAAAATTGAG ATCAGACAAG 
CTAAGGAATG CCTGCGTCAT AATCCAAAAG AGCGTACGAG GATGGGTGCA AAAGAAGAAG 
TTTAGCCGGG CCCGGCGTTC GGCCATTGTC ATACAGCAGT ATTTCCGGGG CCAGAGAGCT 
GTTAGGCAAG CAATAAGTGG AAAAGCCCTG AAGCATGCTT GGGCAGCAAT CATTATCCAG 
AAATATGTTC GGGGATTCTT AGTCCGTCGC ATCTACCAAC TTATCCTGGT GGCCACAGTG 
ACCATTCAGT CATTCACACG TGGTTACTTG GCTAGGAAGA GATATCACAA GATGCGAGAA 
GAGCACAAGG CTTTAATACT GCAGAAATAC GCCCGTGCTT GGCTGGCAAG GCGAAGGTTC 
CAGAACGTCC GTCGTTTTGT GCTCAACATC CAGCTTTCCT ACAGAGTGCA GCGCCTGCAG 
AAAAAACTTG AAGATCAGAA CAAAGAGAAC CATGGGCTGT TGGAACGTCT GACAAGCCTT 
GCGTCGTCAC ACACACACGA TGTGGATAAA GTGCACAAAC TCGAATCGGA GCTGGAAAAA 
ATTTCCTCCC AAAAGAAACA TCTGGATGAA AAGGGAAAGA AGTACAAGGA AGAAGCTGAG 
GAGAAAATGA CAAAGCTTCA AGCCCAAAAT GCTGAACTTC TTGAGCAGAA ACATCAAATG 
GAAGTAAAAC TCGAGACCAA AACCCAAGAA ATGAAAGATC AGATGGATCA GCTCACTAAC 
CAACTATTCA CTGATGTGGA GAAAGAAGAA AAACAAAGGA TGAAACTTGA GAAACATTTT 
GAAGCCCAGG TTACTGAATT CGACACTATG ATAGAAAAAT TAAAAGATGA AATCCAAGTG 
TTAAAGGAGA GGAATAAGCA TCTCCTGAAG CAGGCAGAAG AAGAGCAACT TGTCAATGAT 
GGTTTAAGAG AAGAGATAGA CCAACTTTCC ATCCAAGTAA AGAAAATACC AACATTTCAA 
ACAGATATTG AGCTTCTTGA AAACCAGAAA AGGGATTTAG AACATCAACT ACAGGAACAA 
AAGCGTGAAA TGAGAGAAAA GTTGGATGAA ATGGCAAAAC AGCTTCTGAA CAGTTTTGAA 
ATAGAGGATG TTAAAGCTAG ACTGGCTGAA GAGGAACTAG GAAATCTAAA TGAAGATGGG 
GAAATATGGT TTGCTTATGA AGGTCTGAAA AAAGCAACCA GAATACTAGA AGCTCATTTT 
CAGACACAGA GAGAGAACTA TGAAAAGGAT ATTGAGTTGT TGAACTTTAA GGTCGAGCAC 
CTCCATGATG ACATTAAACA GATACAGAAT TCTTACATGG AGGAAAAAGA CTCAAAGGAG 
AGCATCACTC TTGAAGTTGA AAGGCTGAGA TCTGATAATA AGGTGGTTAA GGATCTGAGG 
AAAAAGTTGG CTGAACTTGA GGAACAAAAC ATGGACCTAC AGGGCCGTCT CTATGAGCAA 
ACTATGAAAT TGCCTGAAAA ATCTAACCGC AGGAAAAATT ATCCTGATGA GGATGAAGTA 
TCCCTCAAAC TGTGGAATGG TCAGAGAGAA AATGATATGG CAAATGTCAA AATACAAGCA 
ATTCAGACAG ACAATATGTA TATGAAGGAA CAAAATGAAG CAGACACCAG GGACTTTGAA 
GAACAGGTAG ATATGAAAGA CCGAACAATC AGGAAACTTC AAGATCAGAT AAAGGCTCTT 
ACGAAGTCCA TTGAAAAGGA TGATGAAGTA AGGGTGCCAA CAGTGTTTAA AGATTATTTG 
GGAATGCTGG AGTTTGCTTC AGAAGATGAA TCAAAACTTA TCAGGAATCT TATTCTAGAC 
CTCAAACCTC GTGGTGTTGT AGTCAACATG ATCCCAGGCC TGCCAGCTCA CATTATGTTC 
ATGTGTGTGA GGTACACAGA CTACATAAAC AATGCTGAAA GAATAAAATC TCTGATGAAT 
GCTTCCATTA ACAGCATCAA ACAGGTTATT AAGGAACATG CAGATGATTT TGAGATGTTG 
TCATTCTGGC TTTCCAATGT CCACCATTTT CTAAACTGCT TAAAGCAGTA CAGCGGGGAA 
GAGGAATTTA TGAAATATAA CCAGCCCCTT CAGAATAAGA ACTGCTTAAA GAATTTTGAT 
CTGTCAGAAT ATAGACAAAT TATCAGCGAT TTGGCAATCC GGATATATCA TCAGTTCATT 
ACTGTAATGG AGAACAACCT TCAGCCAATG ATAGTGCCTG GCATGCTGGA ATATGAAAGC 
CTTCATGGAA TTTCAGTCTC CAAACCAACA GGATTCCGTA AACGTTCATC TAGTATAGAT 
GACACAGACT CTTACACTAT GACCTCAATT CTACAGCAGC TTAGTTATTT TTATACGACT 
ATGTGCCAGC ATGGCATGGA CCCTGAGCTC CTGAAACAAA CAATGAAGCA GCTTTTTTTC 
CTGATTGCCA CTGTCACCAT CAACAGTCTT TTCCTTCGCA AGGACATGTG CTCGTGTAGA 
AAAGGAATGC AGATTCGGTG TAATATCAGT TACCTTGAAG AATGGCTGCA AGGCAAGAAT 
CTTCAGGGGG CTACAGCCAA AGAAACATTG GAGCCCCTTT CCCAGGCAGC ATGGCTATTA 
CAAGTAAAAA AGATTACAGA TGATGACGCC AAAGAAATCT ATGAACGTTG TACTGCACTT 
TCATCTGTGC AGATTGTAAA GATCCTTAAC TCCTACACTC CAATTGATGA TTTTGAGAAA 
CGCGTAACGC CATCCTTTGT ACGCAAGGTC CAGTCCATGC TAAACAACAG AGAAGACTGC 
TCTCAGTTAA TGCTGGATAG TAAATACCTC TTCCAAGTGA CATTTCCTTT CAGCCCATCT 
CCCCACGCAC TGGAGATGAT TCAAATTCCC AGCAGTCTGA AACTTGGCTT CCTAACAAGA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815050.1
CDS	69..5234
Translation	MLHSDPQHSKQKFNRVWIPDAEEVWRSAEITKDLKIGDTVLHLCLEDGTHLKYPVEPEKQVLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESNVIYTYSGIILVAINPYKELPIYGDAIIHAYSGQNMGDIDPHIFAVAEEAYKQMARNNRNQSIIVSGESGAGKTVSARYTMRYFATVSKTSSKAHVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYKIIGANLRTYLLEKSRVVFQSENERNYHIFYQLCASANKPEFEHLHLTRAEEFNYTSMGGCTVITGVDDKKDMLDTQKTFALLGLGTDFQMDVFKVVAAILHLGNVEIKRVSDDRSSVDVNDKHLKIFCDLLNLEASKVAQWLCNRRIVTVSDTVIKPMTKQQADNARDALAKKIYSHLFDYVIDKINNALQYPGKQHTFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQIVIDLIEAKMGILDLLDEECLLPQGTDENWLQKLYNNYLNKNPLFEKPRMSNRSFIIQHFADKVEYKCDGFLEKNRDTVYDVLIDLLRTSKFQLCSSFFQDNTSKSAQFSSGIQVKSARPAIKSSNKEFRMTVGSKFRNSLCLLMETLNATTPHYVRCIKPNDLKLPFEFDAKRVVQQLRACGVLETIRISAQSYPSRWTYIEFFGRYSILMTQQELSLNDKKYICKIVLQRLIQDSNQYQFGRTKIFFRAGQVAYLEKLRSDKLRNACVIIQKSVRGWVQKKKFSRARRSAIVIQQYFRGQRAVRQAISGKALKHAWAAIIIQKYVRGFLVRRIYQLILVATVTIQSFTRGYLARKRYHKMREEHKALILQKYARAWLARRRFQNVRRFVLNIQLSYRVQRLQKKLEDQNKENHGLLERLTSLASSHTHDVDKVHKLESELEKISSQKKHLDEKGKKYKEEAEEKMTKLQAQNAELLEQKHQMEVKLETKTQEMKDQMDQLTNQLFTDVEKEEKQRMKLEKHFEAQVTEFDTMIEKLKDEIQVLKERNKHLLKQAEEEQLVNDGLREEIDQLSIQVKKIPTFQTDIELLENQKRDLEHQLQEQKREMREKLDEMAKQLLNSFEIEDVKARLAEEELGNLNEDGEIWFAYEGLKKATRILEAHFQTQRENYEKDIELLNFKVEHLHDDIKQIQNSYMEEKDSKESITLEVERLRSDNKVVKDLRKKLAELEEQNMDLQGRLYEQTMKLPEKSNRRKNYPDEDEVSLKLWNGQRENDMANVKIQAIQTDNMYMKEQNEADTRDFEEQVDMKDRTIRKLQDQIKALTKSIEKDDEVRVPTVFKDYLGMLEFASEDESKLIRNLILDLKPRGVVVNMIPGLPAHIMFMCVRYTDYINNAERIKSLMNASINSIKQVIKEHADDFEMLSFWLSNVHHFLNCLKQYSGEEEFMKYNQPLQNKNCLKNFDLSEYRQIISDLAIRIYHQFITVMENNLQPMIVPGMLEYESLHGISVSKPTGFRKRSSSIDDTDSYTMTSILQQLSYFYTTMCQHGMDPELLKQTMKQLFFLIATVTINSLFLRKDMCSCRKGMQIRCNISYLEEWLQGKNLQGATAKETLEPLSQAAWLLQVKKITDDDAKEIYERCTALSSVQIVKILNSYTPIDDFEKRVTPSFVRKVQSMLNNREDCSQLMLDSKYLFQVTFPFSPSPHALEMIQIPSSLKLGFLTRI

Target ORF information:

RefSeq Version	XM_012959596.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VC (myo5c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959596.2

ORF Insert Sequence:

ATGCTACATA GTGACCCCCA GCACAGCAAG CAAAAGTTCA ACAGGGTCTG GATCCCCGAC 
GCAGAGGAAG TGTGGCGTTC TGCTGAAATT ACAAAAGATC TGAAGATCGG GGACACTGTG 
CTCCATTTGT GTTTGGAAGA TGGAACTCAC TTGAAGTATC CAGTGGAACC TGAAAAGCAG 
GTTCTCCCCC CTCTAAGAAA TCCAGATATT CTTGTTGGTG AAAATGACCT CACAGCCCTC 
AGTTATTTGC ACGAGCCAGC TGTTCTGCAC AATCTCAAAG TTCGATTTGT GGAGTCCAAC 
GTTATTTATA CATACAGTGG TATTATCTTG GTTGCAATCA ATCCATACAA GGAGCTCCCA 
ATCTATGGAG ATGCAATTAT CCATGCATAC AGTGGGCAAA ATATGGGAGA CATAGATCCG 
CATATATTTG CTGTTGCAGA AGAGGCCTAC AAACAAATGG CAAGGAACAA TAGAAATCAG 
TCCATTATTG TTAGTGGAGA ATCTGGAGCA GGAAAGACAG TGTCAGCTCG ATATACTATG 
AGGTACTTTG CCACAGTCAG TAAAACAAGC AGCAAAGCAC ACGTGGAAGA TAAAGTGCTG 
GCATCCAATC CAATAACAGA GGCTATTGGA AATGCAAAAA CCACTCGAAA TGATAACAGC 
AGTCGCTTTG GAAAGTATAC TGAAATCAGT TTTGATAGAA GGTATAAAAT TATTGGTGCA 
AACTTGAGAA CGTATCTTCT GGAAAAATCC CGGGTAGTCT TTCAGTCAGA AAATGAAAGG 
AATTACCACA TATTTTACCA GCTTTGTGCT TCTGCCAACA AACCGGAATT TGAACACCTT 
CATCTAACGA GAGCAGAAGA GTTCAACTAC ACTTCAATGG GTGGCTGCAC AGTTATAACG 
GGAGTTGATG ACAAAAAAGA TATGTTGGAT ACACAAAAGA CATTTGCTTT ATTAGGTTTA 
GGCACAGACT TTCAGATGGA TGTATTTAAA GTCGTAGCAG CTATCCTGCA CTTAGGAAAT 
GTGGAGATCA AGCGTGTCAG TGATGATAGA TCCTCTGTTG ATGTAAATGA CAAACACTTG 
AAGATATTTT GTGATCTTCT GAATCTAGAA GCCTCAAAGG TGGCACAGTG GCTGTGCAAC 
CGGAGAATTG TGACTGTATC AGACACTGTT ATAAAACCCA TGACCAAACA GCAAGCTGAC 
AATGCAAGGG ATGCTTTGGC CAAGAAAATC TACTCTCACC TTTTTGATTA TGTCATCGAT 
AAAATAAATA ATGCATTACA GTACCCAGGG AAACAACATA CATTTATAGG TGTCCTTGAT 
ATTTATGGGT TCGAAACATT TGAAATTAAC AGTTTTGAAC AGTTTTGCAT CAATTATGCC 
AATGAGAAGC TGCAGCAACA GTTCAACCTT CATGTTTTCA AGCTTGAGCA GGAAGAATAT 
ATGAAGGAAG ACATCCCATG GACTCTTATT GACTTTTATG ATAACCAAAT AGTAATTGAC 
CTCATTGAAG CAAAGATGGG CATTTTGGAC CTGCTAGATG AAGAGTGCCT GCTGCCGCAA 
GGAACAGACG AAAACTGGTT ACAGAAGCTG TATAATAACT ACCTTAACAA GAACCCCTTG 
TTTGAAAAGC CCCGAATGTC CAACAGATCA TTCATTATAC AGCACTTTGC AGATAAGGTA 
GAATATAAGT GTGATGGATT TTTGGAGAAA AACCGAGACA CTGTGTATGA TGTTTTAATT 
GATCTTCTGA GGACTAGTAA GTTTCAGCTG TGCTCCAGCT TTTTTCAAGA CAACACAAGC 
AAGTCAGCAC AATTCAGTTC TGGAATCCAG GTGAAATCTG CAAGACCTGC GATAAAATCA 
TCAAATAAAG AGTTCAGAAT GACTGTGGGA AGCAAGTTTC GGAACTCGTT ATGTTTGCTC 
ATGGAGACCC TTAATGCAAC AACACCACAC TATGTACGAT GCATAAAGCC CAACGATTTA 
AAACTTCCAT TTGAGTTTGA TGCCAAAAGA GTAGTTCAAC AGCTACGAGC CTGTGGAGTT 
CTAGAAACCA TAAGAATCAG TGCACAAAGC TACCCTTCCA GGTGGACATA CATTGAGTTC 
TTTGGCCGTT ACAGCATTCT TATGACTCAG CAGGAACTGT CCCTGAATGA TAAGAAATAC 
ATTTGCAAAA TAGTACTGCA AAGACTGATC CAGGATTCCA ACCAATATCA GTTTGGAAGA 
ACCAAAATCT TCTTCAGAGC TGGCCAGGTG GCTTACTTAG AAAAATTGAG ATCAGACAAG 
CTAAGGAATG CCTGCGTCAT AATCCAAAAG AGCGTACGAG GATGGGTGCA AAAGAAGAAG 
TTTAGCCGGG CCCGGCGTTC GGCCATTGTC ATACAGCAGT ATTTCCGGGG CCAGAGAGCT 
GTTAGGCAAG CAATAAGTGG AAAAGCCCTG AAGCATGCTT GGGCAGCAAT CATTATCCAG 
AAATATGTTC GGGGATTCTT AGTCCGTCGC ATCTACCAAC TTATCCTGGT GGCCACAGTG 
ACCATTCAGT CATTCACACG TGGTTACTTG GCTAGGAAGA GATATCACAA GATGCGAGAA 
GAGCACAAGG CTTTAATACT GCAGAAATAC GCCCGTGCTT GGCTGGCAAG GCGAAGGTTC 
CAGAACGTCC GTCGTTTTGT GCTCAACATC CAGCTTTCCT ACAGAGTGCA GCGCCTGCAG 
AAAAAACTTG AAGATCAGAA CAAAGAGAAC CATGGGCTGT TGGAACGTCT GACAAGCCTT 
GCGTCGTCAC ACACACACGA TGTGGATAAA GTGCACAAAC TCGAATCGGA GCTGGAAAAA 
ATTTCCTCCC AAAAGAAACA TCTGGATGAA AAGGGAAAGA AGTACAAGGA AGAAGCTGAG 
GAGAAAATGA CAAAGCTTCA AGCCCAAAAT GCTGAACTTC TTGAGCAGAA ACATCAAATG 
GAAGTAAAAC TCGAGACCAA AACCCAAGAA ATGAAAGATC AGATGGATCA GCTCACTAAC 
CAACTATTCA CTGATGTGGA GAAAGAAGAA AAACAAAGGA TGAAACTTGA GAAACATTTT 
GAAGCCCAGG TTACTGAATT CGACACTATG ATAGAAAAAT TAAAAGATGA AATCCAAGTG 
TTAAAGGAGA GGAATAAGCA TCTCCTGAAG CAGGCAGAAG AAGAGCAACT TGTCAATGAT 
GGTTTAAGAG AAGAGATAGA CCAACTTTCC ATCCAAGTAA AGAAAATACC AACATTTCAA 
ACAGATATTG AGCTTCTTGA AAACCAGAAA AGGGATTTAG AACATCAACT ACAGGAACAA 
AAGCGTGAAA TGAGAGAAAA GTTGGATGAA ATGGCAAAAC AGCTTCTGAA CAGTTTTGAA 
ATAGAGGATG TTAAAGCTAG ACTGGCTGAA GAGGAACTAG GAAATCTAAA TGAAGATGGG 
GAAATATGGT TTGCTTATGA AGGTCTGAAA AAAGCAACCA GAATACTAGA AGCTCATTTT 
CAGACACAGA GAGAGAACTA TGAAAAGGAT ATTGAGTTGT TGAACTTTAA GGTCGAGCAC 
CTCCATGATG ACATTAAACA GATACAGAAT TCTTACATGG AGGAAAAAGA CTCAAAGGAG 
AGCATCACTC TTGAAGTTGA AAGGCTGAGA TCTGATAATA AGGTGGTTAA GGATCTGAGG 
AAAAAGTTGG CTGAACTTGA GGAACAAAAC ATGGACCTAC AGGGCCGTCT CTATGAGCAA 
ACTATGAAAT TGCCTGAAAA ATCTAACCGC AGGAAAAATT ATCCTGATGA GGATGAAGTA 
TCCCTCAAAC TGTGGAATGG TCAGAGAGAA AATGATATGG CAAATGTCAA AATACAAGCA 
ATTCAGACAG ACAATATGTA TATGAAGGAA CAAAATGAAG CAGACACCAG GGACTTTGAA 
GAACAGGTAG ATATGAAAGA CCGAACAATC AGGAAACTTC AAGATCAGAT AAAGGCTCTT 
ACGAAGTCCA TTGAAAAGGA TGATGAAGTA AGGGTGCCAA CAGTGTTTAA AGATTATTTG 
GGAATGCTGG AGTTTGCTTC AGAAGATGAA TCAAAACTTA TCAGGAATCT TATTCTAGAC 
CTCAAACCTC GTGGTGTTGT AGTCAACATG ATCCCAGGCC TGCCAGCTCA CATTATGTTC 
ATGTGTGTGA GGTACACAGA CTACATAAAC AATGCTGAAA GAATAAAATC TCTGATGAAT 
GCTTCCATTA ACAGCATCAA ACAGGTTATT AAGGAACATG CAGATGATTT TGAGATGTTG 
TCATTCTGGC TTTCCAATGT CCACCATTTT CTAAACTGCT TAAAGCAGTA CAGCGGGGAA 
GAGGAATTTA TGAAATATAA CCAGCCCCTT CAGAATAAGA ACTGCTTAAA GAATTTTGAT 
CTGTCAGAAT ATAGACAAAT TATCAGCGAT TTGGCAATCC GGATATATCA TCAGTTCATT 
ACTGTAATGG AGAACAACCT TCAGCCAATG ATAGTGCCTG GCATGCTGGA ATATGAAAGC 
CTTCATGGAA TTTCAGTCTC CAAACCAACA GGATTCCGTA AACGTTCATC TAGTATAGAT 
GACACAGACT CTTACACTAT GACCTCAATT CTACAGCAGC TTAGTTATTT TTATACGACT 
ATGTGCCAGC ATGGCATGGA CCCTGAGCTC CTGAAACAAA CAATGAAGCA GCTTTTTTTC 
CTGATTGCCA CTGTCACCAT CAACAGTCTT TTCCTTCGCA AGGACATGTG CTCGTGTAGA 
AAAGGAATGC AGATTCGGTG TAATATCAGT TACCTTGAAG AATGGCTGCA AGGCAAGAAT 
CTTCAGGGGG CTACAGCCAA AGAAACATTG GAGCCCCTTT CCCAGGCAGC ATGGCTATTA 
CAAGTAAAAA AGATTACAGA TGATGACGCC AAAGAAATCT ATGAACGTTG TACTGCACTT 
TCATCTGTGC AGATTGTAAA GATCCTTAAC TCCTACACTC CAATTGATGA TTTTGAGAAA 
CGCGTAACGC CATCCTTTGT ACGCAAGGTC CAGTCCATGC TAAACAACAG AGAAGACTGC 
TCTCAGTTAA TGCTGGATAG TAAATACCTC TTCCAAGTGA CATTTCCTTT CAGCCCATCT 
CCCCACGCAC TGGAGATGAT TCAAATTCCC AGCAGTCTGA AACTTGGCTT CCTAACAAGA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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myo5c cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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