myh4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	myh4
Entrez Gene ID	407946
Full Name	myosin, heavy chain 4, skeletal muscle
Synonyms	E3,mhc,myh2b,myhc-2b,myhc-iib,myhe3
General protein information	Preferred Names myosin, heavy chain 4, skeletal muscle Names myosin, heavy chain 4, skeletal muscle MHC A7 MHC E3 myhc E3 myosin heavy chain
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001244.1	NP_001001244.1	myosin, heavy chain 4, skeletal muscle
XM_031890312.1	XP_031746172.1	myosin, heavy chain 4, skeletal muscle isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH1	NP_005954.3
Xenopus tropicalis (tropical clawed frog)	myh8	XP_004916771.1
Xenopus tropicalis (tropical clawed frog)	LOC100495802	XP_002935255.1
Bos taurus (cattle)	MYH1	NP_776542.1
Xenopus tropicalis (tropical clawed frog)	myh4	NP_001001244.1
Xenopus tropicalis (tropical clawed frog)	myh2	NP_001006805.1
Xenopus tropicalis (tropical clawed frog)	LOC100495968	XP_002935256.2
Pan troglodytes (chimpanzee)	MYH1	XP_003953177.1
Canis lupus familiaris (dog)	MYH1	NP_001107189.1
Mus musculus (house mouse)	Myh1	NP_109604.1
Rattus norvegicus (Norway rat)	Myh1	NP_001128630.1
Xenopus tropicalis (tropical clawed frog)	LOC100495638	XP_002935254.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myh4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myh4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa00939	XM_031890312.1 Latest version!	Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa00939	NM_001001244.1 Latest version!	Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myh4 ( XM_031890312.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031746172.1 Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa00939
Clone ID Related Accession (Same CDS sequence)	NM_001001244.1 , XM_031890312.1
Accession Version	XM_031890312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin, heavy chain 4, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGATG GAGAAATGTC CATCTTCGGC GAGGCCGCAC AATTTCTCAG AAAAAGTGAG 
AAAGAGCGTT TGGAGGCTCA GAGCAAGCCT TTTGATGCCA AAAACACTGT ATTTGTAGAT 
GATGTTAAGG AACTCTATGT CAAAGGTATG GTTACAGCCA GGGAAGATGG CAAAATAACT 
GTGAAGACCG ATGATGGAAG AACAGTAACC GTCAAAGAGA ACCAAATTTA CCCCCAGAAC 
CCTCCCAAGT TTGATAAAAT TGAAGACATG GCTATGATGA CCCATTTGAA TGAGGCTTCT 
GTCCTGTATA ACCTGAAAGA GCGTTATGCT GCATGGATGA TCTATACCTA CTCTGGCCTT 
TTCTGCGCCA CTGTTAACCC CTACAAGTGG CTGCCAGTGT ACAACCCTGA AGTTGTTGCC 
GGTTACAGAG GGAAAAAGCG TATGGAAGCT CCCCCTCACA TTTTCTCCCT TTCTGATAAT 
GCCTATCAGG CCATGTTAAC AGATCGTGAA AACCAGTCTG TCCTTATTAC TGGTGAATCT 
GGTGCAGGAA AGACTGTGAA CACCAAGCGT GTCATCCAGT ATTTTGCAAC AATTGCAGCC 
CTTGGAGACA AGAAGAAGGA ATCCTCCAAC AGCTTGCAGG GAAATCTGGA AGATCAGATC 
ATTCAGGCCA ACCCTCTGCT GGAAGCCTTT GGTAATGCCA AGACTGTGAG AAATGACAAC 
TCCTCTCGTT TTGGTAAATT CATCAGAATC CATTTTGGAA CTACAGGAAA ACTGTCTTCA 
GCTGATATTG AAACATATCT GTTGGAAAAG TCCAGAGTTA CTTTCCAGCT TTCAGCAGAA 
AGAAGCTATC ACATCTTCTA TCAGATCTTA ACAAATAAGA AGCCAGAAAT TGTTGAGATG 
CTTCTACTCA CTACTAACCC ATATGACTAC CCATCCATCA GTCAGGGTGA GCTTGTTGTG 
AAGAGCATTA ATGATGAAGA GGAGTTGATG GCAACAGATA GTGCCATTGA CGTTTTGGGT 
TTCAATCAAG AAGAAAAGAT GGGTATTTAC AAAATGACTG GTGCTGTCAT GCATCATGGC 
AACTTGAAGT TCAAGCAAAA GCAAAGAGAG GAGCAGGCTG AGCCTGATAG CACTGAGGTG 
GCTGACAAAA TTGCTTATCT GTTGGGCCTC AACTCTGCGG ATTTGCTCAA AGGTTTGTGC 
TACCCAAGAG TCAAGGTCGG CAATGAATTT GTTACCAAAG GACAGACTGT ACCACAGGTC 
TATAACTCTG TTGGTGCCCT GTGCAAGTCT GTTTTTGAAA AGCTGTTCTT GTGGATGGTC 
ACTCGTATTA ACCAACAGCT GGATACTAAG CAACCAAGAC AGTTCTTCAT TGGTGTGCTG 
GACATTGCTG GATTTGAAAT CTTTGATTTT AACAGCTTGG AGCAGCTCTG CATCAACTTT 
ACCAATGAAA AACTGCAACA GTTTTTCAAT CACCACATGT TCGTCCTGGA GCAGGAGGAA 
TACAAGAAGG AAGGAATTGA TTGGGAGTTT ATTGACTTCG GTATGGATTT GGCTGCCTGC 
ATTGAGCTTA TTGAGAAGCC ATTGGGTATC TTCTCCATCC TTGAAGAGGA GTGCATGTTC 
CCCAAAGCCA CTGATACTTC CTTCAAGAAC AAGCTATATG AGCAACATCT GGGCAAATGC 
AAGAACTTTG AGAAGCCCAA GCCTGGCAAA GGAAAGGCTG AAGCTCACTT CTCGCTTGTG 
CATTATGCTG GCACTGTGGA TTACAACATC AGTGGATGGC TTGAAAAGAA CAAGGACCCA 
CTGAACGAGT CAGTTGTCCA GCTCTACCAG AAGTCTTCTG TCAAACTGCT GTCCTTGCTC 
TACTCCAGCT ATGCTGCAAC TGACGGTGAT GCTGGTGGCA AAGGTGGTAA GAAAAAGAAG 
GGATCCTCTT TCCAGACTGT GTCTGGTCTC TTCAGGGAAA ATCTGAACAA ACTGATGACA 
AACTTGAGAA GCACTCACCC TCACTTTGTA CGTTGTTTGA TTCCCAATGA GACCAAGACT 
CCTGGTATCA TGGACAACCA TCTCCTTATT CACCAGCTGA GATGTAATGG TGTGCTGGAA 
GGTATTAGAA TCTGCAGGAA AGGATTCCCA AGCAGAATCC TCTATGGTGA TTTCAAACAA 
CGTTACAAAG TTCTGAATGC CAGTGCAATT CCAGAAGGGC AATTTATTGA CAGCAAGAAG 
GCTTGTGAGA AGCTTCTAGG CTCAATTGAT GTCGACCATA CTCAGTATAA ATTTGGACAC 
ACCAAGGTAT TTTTCAAAGC TGGTCTTTTG GGTACTTTGG AAGAAATGAG AGATGAAAAA 
TTGGCCCAAC TTATCACTCG CACCCAAGCT CTTTGCAGAG GATATTTGAT GAGGCTTGAG 
TTCACCAAAA TGATGGAAAG AAGGGAGGCC ATATATGTCA TCCAGTACAA TCTGAGGTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAACTGTACT TCAAGATCAA GCCTCTTCTG 
AAGAGCGCTG AGACTGAGAA AGAAATGGCA AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAAGCCCTCG TGAAGGCAGA AGCAAGGAAG AAGGAACTGG AGGAGAAAAT GGTTGCCATG 
CTCCAGGAAA AGAATGATCT AGTCCTGCAG GTTCAATCGG AAAGTGAAAC CTTGGCTGAT 
TCTGAGGAGA GATGTGAAGG GCTCATCAAA GTCAAAATTC AGCTGGAGTC TAAAATAAAG 
GAATTAACAG AAAGACTTGA AGATGAAGAA GAAAGCAATG CAGAGCTAAC AGCAAAGAAG 
AGAAAATTGG AGGATGAATG TTCTGAGCTC AAGAAAGACA TTGACGACCT TGAACTTACT 
TTGGCTAAAG TAGAAAAGGA GAAGCATGCC ACAGAAAACA AGGTTAAAAA CCTTACTGAA 
GAAATGGCAG TTCTCGATGA GAACATTTCC AAACTGTCCA AAGAAAAGAA GGCCCTTCAA 
GAGGCTCACC AACAAACACT TGATGACCTG CAAGCTGAAG AGGACAAAGT CAGTTCTCTG 
TCAAAAGCAA AGACAAAGCT GGAACAGCAA GTAGATGATC TGGAAGGTTC CCTGGAACAA 
GAGAAGAAAC TTCGTCTTGA CCTTGAGAGA GCCAAGAGAA AGCTTGAAGG AGATTTGAAA 
CTGACCCAGG AAACCGTCAT GGACCTTGAG AATGATAAAC AGCAAACTGA AGAAAAACTT 
AAAAAGAAAG ACTTTGAAAT AAGCCAGCTT CAAGGAAAGA TAGAGGATGA ACAGTCCCTG 
GGTACTCAGT TGCAGAAAAA GATCAAGGAA CTGCAGGCTC GAATTGAGGA AGTTGAGGAA 
GAAATTGAAG CTGAACGTGC AGCTCGTGCA AAGGTAGAGA AACAAAGGGC TGATCTTTCC 
AGGGAACTTG AGGAGATCAG TGAGAGACTT GAGGAAGCTG GAGGTGCAAC ATCTGCTCAG 
ATTGAGATGA ACAAAAAACG TGAAGCTGAG TTCCAGAAAC TGAGACGTGA CCTGGAGGAG 
GCAACCCTTC AGCATGAAGC CACTGCTGGT GCCCTGCGCA AAAAACATGC TGACAGTGTA 
GCTGAGCTTG GGGAGCAAAT TGATAACCTG CAGAGGGTTA AACAAAAGCT GGAGAAAGAA 
AAAAGTGAGC TGAAGATGGA GATTGATGAT CTTGCCAGCA ACTTGGAAAA TGTATCCAAA 
TCAAAGGCTA ATCTTGAAAA GGTTAACCGT GTGATTGAGG ACCAACTTAG TGAAGTGAAG 
GCAAAGGATG ATGAACATCA GCGTCTCCTC AACGACCTCA GTACTCAAAA GGCTCGCTTG 
CAGACTGAAA CCGGTGAGCT CTCCCGCCAG CTTGAAGAGA GGGAATCTCT GATTTCTCAG 
CTCTCAAGAG GAAAGCAGGG ATTTACCCAG CAAGTTGAAG AACTCAAGAG GCAGCTTGAA 
GAAGAAACAA AGGCTAAGAA TGCCCTTGCT CATGCTCTGC AATCAGCTCG TCATGACTGT 
GACTTGCTTA GAGAACAATT TGAGGAAGAA CAGGAGGCCA AGGCTGAACT TCAGCGCTCA 
CTGTCCAAAG CCAATGGTGA AGTTTCACAA TGGAGGACCA AATATGAAAC TGATGCAATT 
CAGCGTACTG AGGAACTTGA AGAAGCCAAG AAGAAACTTG CTCAGCGTTT ACAGGACGCT 
GAGGAGCAAG TTGAGGCTGT GAATTCCAAA TGCGCCTCCT TGGAAAAGAC TAAGCAGAGA 
CTGCAATCCG AAGTGGAGGA CTTGATGGTT GATGTTGAGA GAGCAAATAG TGCAGCAGCT 
GCTCTTGATA AGAAGCAGAG AAACTTTGAT AAGGTCCTGG TAGAATGGAA ACAGAAGTAT 
GAAGAGGGTC AGGCTGAATT GGAAGCAGCT CTTAAGGAAT CTCGCAGCCT GAGCACTGAG 
ATCTTCAAGA TGAAGAATGC TTATGAGGAA GCTCTGGAGC ATGTTGAAAC TCTGAAACGT 
GAAAACAAGA ACCTTCAACA GGAGATTTCA GATCTGACTG AACAGATTGG TGAGAGTGGA 
AAATCAGTTG TTGAATTGGA AAAGGCCAAG AAGCAGGTGG AACAAGAGAA GAATGATCTC 
CAAGCTGCTC TAGAAGAAGC AGAGGGATCT TTGGAACATG AAGAAGCCAA GATTCTGCGC 
ATCCAGCTTG AACTGAACCA GGTCAAATCT GAAGTGGACA GGAAAATTGC AGAGAAGGAT 
GAGGAAATTG AGCAACTGAA GAGAAACAGC CAGAGGTCCA TTGACACCAT GCAGAGCACA 
CTGGACTCTG AAATCAGGAG CAGAAATGAT GCACTGAGAC TGAAGAAGAA GATGGAAGGT 
GATCTGAATG AGCTTGAGAT CCAGCTGGGT CATGCCAACC GTCAGGCTTC TGAAGCACAG 
AAACAGCTTA GGAATGTACA GGGACAGTTC AAGGAGACCC AGCTCCAGCT GGATGAAGCA 
ATCAGGGCAC AGGAAGACCT GAAGGAACAG GTTGCTGTGA CTGAGCGCAG AAATAACTTG 
TTTCAAGCTG AAATTGAAGA AAACAGAGCT GGTCTGGAAC AGACAGAAAG ATCTCGTAAG 
ATTGCAGAAC AGGAGCTCCT AGATGCAAGT GAACGTCTTC AGCTGTTGCA TTCCCAGAAC 
ACAAGTCTCA TTAACAGCAA GAAGAAGCTT GAAAGTGACA TCTGTCAACT ACAGAATGAG 
GCCGAGGAAG CTGTCCAAGA AGCCAGAAAT GCTGAAGAGA AGGCAAAGAA GGCCATAACA 
GATGCTGCCC TGATGGCAGA AGAGTTGAAG AAGGAGCAGG ACACCAGTGC TCACTTAGAA 
AGGATGAAGA AGAACCTTGA ACAGACTGTG AAGGACCTGC AGCATCGCCT TGATGAAGCT 
GAACAGCTGG CAATGAAGGG TGGCAAGAAG CAGCTCCAGA AACTGGAATC CAGGGTACGA 
GAACTGGAGA ATGAGCTGGA TAATGAACAG AAACATGGTG TTGAGGCTGT GAAAGGTGTT 
CGTAAATATG AGAGGAGAGT GAAGGAACTC ACATACCAGA CTGAGGAAGA TAAGAAGAAC 
ATTTTGAGAC TGCAGGACCT GGTAGATAAA CTTCAGTCTA AGGTTAAAGC CTACAAGAGG 
CAGGCTGAAG AAGCTGAGGA ACAAGCCAAC ACCCATTTGG GCCGCTTCCG TAAGGTACAG 
CACGAGCTTG AAGAAGCAGA AGAAAGAGCC GATATTGCCG AGTCCCAAGT GAATAAGCTC 
AGGACAAAGA GCCGTGATAT TGGAGGCAAG AAAGAGTCTG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031746172.1
CDS	73..5880
Translation	MGDGEMSIFGEAAQFLRKSEKERLEAQSKPFDAKNTVFVDDVKELYVKGMVTAREDGKITVKTDDGRTVTVKENQIYPQNPPKFDKIEDMAMMTHLNEASVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYNPEVVAGYRGKKRMEAPPHIFSLSDNAYQAMLTDRENQSVLITGESGAGKTVNTKRVIQYFATIAALGDKKKESSNSLQGNLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLSSADIETYLLEKSRVTFQLSAERSYHIFYQILTNKKPEIVEMLLLTTNPYDYPSISQGELVVKSINDEEELMATDSAIDVLGFNQEEKMGIYKMTGAVMHHGNLKFKQKQREEQAEPDSTEVADKIAYLLGLNSADLLKGLCYPRVKVGNEFVTKGQTVPQVYNSVGALCKSVFEKLFLWMVTRINQQLDTKQPRQFFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYEQHLGKCKNFEKPKPGKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNESVVQLYQKSSVKLLSLLYSSYAATDGDAGGKGGKKKKGSSFQTVSGLFRENLNKLMTNLRSTHPHFVRCLIPNETKTPGIMDNHLLIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDVDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQALCRGYLMRLEFTKMMERREAIYVIQYNLRSFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEALVKAEARKKELEEKMVAMLQEKNDLVLQVQSESETLADSEERCEGLIKVKIQLESKIKELTERLEDEEESNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAVLDENISKLSKEKKALQEAHQQTLDDLQAEEDKVSSLSKAKTKLEQQVDDLEGSLEQEKKLRLDLERAKRKLEGDLKLTQETVMDLENDKQQTEEKLKKKDFEISQLQGKIEDEQSLGTQLQKKIKELQARIEEVEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKLRRDLEEATLQHEATAGALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNLENVSKSKANLEKVNRVIEDQLSEVKAKDDEHQRLLNDLSTQKARLQTETGELSRQLEERESLISQLSRGKQGFTQQVEELKRQLEEETKAKNALAHALQSARHDCDLLREQFEEEQEAKAELQRSLSKANGEVSQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEQVEAVNSKCASLEKTKQRLQSEVEDLMVDVERANSAAAALDKKQRNFDKVLVEWKQKYEEGQAELEAALKESRSLSTEIFKMKNAYEEALEHVETLKRENKNLQQEISDLTEQIGESGKSVVELEKAKKQVEQEKNDLQAALEEAEGSLEHEEAKILRIQLELNQVKSEVDRKIAEKDEEIEQLKRNSQRSIDTMQSTLDSEIRSRNDALRLKKKMEGDLNELEIQLGHANRQASEAQKQLRNVQGQFKETQLQLDEAIRAQEDLKEQVAVTERRNNLFQAEIEENRAGLEQTERSRKIAEQELLDASERLQLLHSQNTSLINSKKKLESDICQLQNEAEEAVQEARNAEEKAKKAITDAALMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLAMKGGKKQLQKLESRVRELENELDNEQKHGVEAVKGVRKYERRVKELTYQTEEDKKNILRLQDLVDKLQSKVKAYKRQAEEAEEQANTHLGRFRKVQHELEEAEERADIAESQVNKLRTKSRDIGGKKESEE

Target ORF information:

RefSeq Version	XM_031890312.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031890312.1

ORF Insert Sequence:

ATGGGTGATG GAGAAATGTC CATCTTCGGC GAGGCCGCAC AATTTCTCAG AAAAAGTGAG 
AAAGAGCGTT TGGAGGCTCA GAGCAAGCCT TTTGATGCCA AAAACACTGT ATTTGTAGAT 
GATGTTAAGG AACTCTATGT CAAAGGTATG GTTACAGCCA GGGAAGATGG CAAAATAACT 
GTGAAGACCG ATGATGGAAG AACAGTAACC GTCAAAGAGA ACCAAATTTA CCCCCAGAAC 
CCTCCCAAGT TTGATAAAAT TGAAGACATG GCTATGATGA CCCATTTGAA TGAGGCTTCT 
GTCCTGTATA ACCTGAAAGA GCGTTATGCT GCATGGATGA TCTATACCTA CTCTGGCCTT 
TTCTGCGCCA CTGTTAACCC CTACAAGTGG CTGCCAGTGT ACAACCCTGA AGTTGTTGCC 
GGTTACAGAG GGAAAAAGCG TATGGAAGCT CCCCCTCACA TTTTCTCCCT TTCTGATAAT 
GCCTATCAGG CCATGTTAAC AGATCGTGAA AACCAGTCTG TCCTTATTAC TGGTGAATCT 
GGTGCAGGAA AGACTGTGAA CACCAAGCGT GTCATCCAGT ATTTTGCAAC AATTGCAGCC 
CTTGGAGACA AGAAGAAGGA ATCCTCCAAC AGCTTGCAGG GAAATCTGGA AGATCAGATC 
ATTCAGGCCA ACCCTCTGCT GGAAGCCTTT GGTAATGCCA AGACTGTGAG AAATGACAAC 
TCCTCTCGTT TTGGTAAATT CATCAGAATC CATTTTGGAA CTACAGGAAA ACTGTCTTCA 
GCTGATATTG AAACATATCT GTTGGAAAAG TCCAGAGTTA CTTTCCAGCT TTCAGCAGAA 
AGAAGCTATC ACATCTTCTA TCAGATCTTA ACAAATAAGA AGCCAGAAAT TGTTGAGATG 
CTTCTACTCA CTACTAACCC ATATGACTAC CCATCCATCA GTCAGGGTGA GCTTGTTGTG 
AAGAGCATTA ATGATGAAGA GGAGTTGATG GCAACAGATA GTGCCATTGA CGTTTTGGGT 
TTCAATCAAG AAGAAAAGAT GGGTATTTAC AAAATGACTG GTGCTGTCAT GCATCATGGC 
AACTTGAAGT TCAAGCAAAA GCAAAGAGAG GAGCAGGCTG AGCCTGATAG CACTGAGGTG 
GCTGACAAAA TTGCTTATCT GTTGGGCCTC AACTCTGCGG ATTTGCTCAA AGGTTTGTGC 
TACCCAAGAG TCAAGGTCGG CAATGAATTT GTTACCAAAG GACAGACTGT ACCACAGGTC 
TATAACTCTG TTGGTGCCCT GTGCAAGTCT GTTTTTGAAA AGCTGTTCTT GTGGATGGTC 
ACTCGTATTA ACCAACAGCT GGATACTAAG CAACCAAGAC AGTTCTTCAT TGGTGTGCTG 
GACATTGCTG GATTTGAAAT CTTTGATTTT AACAGCTTGG AGCAGCTCTG CATCAACTTT 
ACCAATGAAA AACTGCAACA GTTTTTCAAT CACCACATGT TCGTCCTGGA GCAGGAGGAA 
TACAAGAAGG AAGGAATTGA TTGGGAGTTT ATTGACTTCG GTATGGATTT GGCTGCCTGC 
ATTGAGCTTA TTGAGAAGCC ATTGGGTATC TTCTCCATCC TTGAAGAGGA GTGCATGTTC 
CCCAAAGCCA CTGATACTTC CTTCAAGAAC AAGCTATATG AGCAACATCT GGGCAAATGC 
AAGAACTTTG AGAAGCCCAA GCCTGGCAAA GGAAAGGCTG AAGCTCACTT CTCGCTTGTG 
CATTATGCTG GCACTGTGGA TTACAACATC AGTGGATGGC TTGAAAAGAA CAAGGACCCA 
CTGAACGAGT CAGTTGTCCA GCTCTACCAG AAGTCTTCTG TCAAACTGCT GTCCTTGCTC 
TACTCCAGCT ATGCTGCAAC TGACGGTGAT GCTGGTGGCA AAGGTGGTAA GAAAAAGAAG 
GGATCCTCTT TCCAGACTGT GTCTGGTCTC TTCAGGGAAA ATCTGAACAA ACTGATGACA 
AACTTGAGAA GCACTCACCC TCACTTTGTA CGTTGTTTGA TTCCCAATGA GACCAAGACT 
CCTGGTATCA TGGACAACCA TCTCCTTATT CACCAGCTGA GATGTAATGG TGTGCTGGAA 
GGTATTAGAA TCTGCAGGAA AGGATTCCCA AGCAGAATCC TCTATGGTGA TTTCAAACAA 
CGTTACAAAG TTCTGAATGC CAGTGCAATT CCAGAAGGGC AATTTATTGA CAGCAAGAAG 
GCTTGTGAGA AGCTTCTAGG CTCAATTGAT GTCGACCATA CTCAGTATAA ATTTGGACAC 
ACCAAGGTAT TTTTCAAAGC TGGTCTTTTG GGTACTTTGG AAGAAATGAG AGATGAAAAA 
TTGGCCCAAC TTATCACTCG CACCCAAGCT CTTTGCAGAG GATATTTGAT GAGGCTTGAG 
TTCACCAAAA TGATGGAAAG AAGGGAGGCC ATATATGTCA TCCAGTACAA TCTGAGGTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAACTGTACT TCAAGATCAA GCCTCTTCTG 
AAGAGCGCTG AGACTGAGAA AGAAATGGCA AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAAGCCCTCG TGAAGGCAGA AGCAAGGAAG AAGGAACTGG AGGAGAAAAT GGTTGCCATG 
CTCCAGGAAA AGAATGATCT AGTCCTGCAG GTTCAATCGG AAAGTGAAAC CTTGGCTGAT 
TCTGAGGAGA GATGTGAAGG GCTCATCAAA GTCAAAATTC AGCTGGAGTC TAAAATAAAG 
GAATTAACAG AAAGACTTGA AGATGAAGAA GAAAGCAATG CAGAGCTAAC AGCAAAGAAG 
AGAAAATTGG AGGATGAATG TTCTGAGCTC AAGAAAGACA TTGACGACCT TGAACTTACT 
TTGGCTAAAG TAGAAAAGGA GAAGCATGCC ACAGAAAACA AGGTTAAAAA CCTTACTGAA 
GAAATGGCAG TTCTCGATGA GAACATTTCC AAACTGTCCA AAGAAAAGAA GGCCCTTCAA 
GAGGCTCACC AACAAACACT TGATGACCTG CAAGCTGAAG AGGACAAAGT CAGTTCTCTG 
TCAAAAGCAA AGACAAAGCT GGAACAGCAA GTAGATGATC TGGAAGGTTC CCTGGAACAA 
GAGAAGAAAC TTCGTCTTGA CCTTGAGAGA GCCAAGAGAA AGCTTGAAGG AGATTTGAAA 
CTGACCCAGG AAACCGTCAT GGACCTTGAG AATGATAAAC AGCAAACTGA AGAAAAACTT 
AAAAAGAAAG ACTTTGAAAT AAGCCAGCTT CAAGGAAAGA TAGAGGATGA ACAGTCCCTG 
GGTACTCAGT TGCAGAAAAA GATCAAGGAA CTGCAGGCTC GAATTGAGGA AGTTGAGGAA 
GAAATTGAAG CTGAACGTGC AGCTCGTGCA AAGGTAGAGA AACAAAGGGC TGATCTTTCC 
AGGGAACTTG AGGAGATCAG TGAGAGACTT GAGGAAGCTG GAGGTGCAAC ATCTGCTCAG 
ATTGAGATGA ACAAAAAACG TGAAGCTGAG TTCCAGAAAC TGAGACGTGA CCTGGAGGAG 
GCAACCCTTC AGCATGAAGC CACTGCTGGT GCCCTGCGCA AAAAACATGC TGACAGTGTA 
GCTGAGCTTG GGGAGCAAAT TGATAACCTG CAGAGGGTTA AACAAAAGCT GGAGAAAGAA 
AAAAGTGAGC TGAAGATGGA GATTGATGAT CTTGCCAGCA ACTTGGAAAA TGTATCCAAA 
TCAAAGGCTA ATCTTGAAAA GGTTAACCGT GTGATTGAGG ACCAACTTAG TGAAGTGAAG 
GCAAAGGATG ATGAACATCA GCGTCTCCTC AACGACCTCA GTACTCAAAA GGCTCGCTTG 
CAGACTGAAA CCGGTGAGCT CTCCCGCCAG CTTGAAGAGA GGGAATCTCT GATTTCTCAG 
CTCTCAAGAG GAAAGCAGGG ATTTACCCAG CAAGTTGAAG AACTCAAGAG GCAGCTTGAA 
GAAGAAACAA AGGCTAAGAA TGCCCTTGCT CATGCTCTGC AATCAGCTCG TCATGACTGT 
GACTTGCTTA GAGAACAATT TGAGGAAGAA CAGGAGGCCA AGGCTGAACT TCAGCGCTCA 
CTGTCCAAAG CCAATGGTGA AGTTTCACAA TGGAGGACCA AATATGAAAC TGATGCAATT 
CAGCGTACTG AGGAACTTGA AGAAGCCAAG AAGAAACTTG CTCAGCGTTT ACAGGACGCT 
GAGGAGCAAG TTGAGGCTGT GAATTCCAAA TGCGCCTCCT TGGAAAAGAC TAAGCAGAGA 
CTGCAATCCG AAGTGGAGGA CTTGATGGTT GATGTTGAGA GAGCAAATAG TGCAGCAGCT 
GCTCTTGATA AGAAGCAGAG AAACTTTGAT AAGGTCCTGG TAGAATGGAA ACAGAAGTAT 
GAAGAGGGTC AGGCTGAATT GGAAGCAGCT CTTAAGGAAT CTCGCAGCCT GAGCACTGAG 
ATCTTCAAGA TGAAGAATGC TTATGAGGAA GCTCTGGAGC ATGTTGAAAC TCTGAAACGT 
GAAAACAAGA ACCTTCAACA GGAGATTTCA GATCTGACTG AACAGATTGG TGAGAGTGGA 
AAATCAGTTG TTGAATTGGA AAAGGCCAAG AAGCAGGTGG AACAAGAGAA GAATGATCTC 
CAAGCTGCTC TAGAAGAAGC AGAGGGATCT TTGGAACATG AAGAAGCCAA GATTCTGCGC 
ATCCAGCTTG AACTGAACCA GGTCAAATCT GAAGTGGACA GGAAAATTGC AGAGAAGGAT 
GAGGAAATTG AGCAACTGAA GAGAAACAGC CAGAGGTCCA TTGACACCAT GCAGAGCACA 
CTGGACTCTG AAATCAGGAG CAGAAATGAT GCACTGAGAC TGAAGAAGAA GATGGAAGGT 
GATCTGAATG AGCTTGAGAT CCAGCTGGGT CATGCCAACC GTCAGGCTTC TGAAGCACAG 
AAACAGCTTA GGAATGTACA GGGACAGTTC AAGGAGACCC AGCTCCAGCT GGATGAAGCA 
ATCAGGGCAC AGGAAGACCT GAAGGAACAG GTTGCTGTGA CTGAGCGCAG AAATAACTTG 
TTTCAAGCTG AAATTGAAGA AAACAGAGCT GGTCTGGAAC AGACAGAAAG ATCTCGTAAG 
ATTGCAGAAC AGGAGCTCCT AGATGCAAGT GAACGTCTTC AGCTGTTGCA TTCCCAGAAC 
ACAAGTCTCA TTAACAGCAA GAAGAAGCTT GAAAGTGACA TCTGTCAACT ACAGAATGAG 
GCCGAGGAAG CTGTCCAAGA AGCCAGAAAT GCTGAAGAGA AGGCAAAGAA GGCCATAACA 
GATGCTGCCC TGATGGCAGA AGAGTTGAAG AAGGAGCAGG ACACCAGTGC TCACTTAGAA 
AGGATGAAGA AGAACCTTGA ACAGACTGTG AAGGACCTGC AGCATCGCCT TGATGAAGCT 
GAACAGCTGG CAATGAAGGG TGGCAAGAAG CAGCTCCAGA AACTGGAATC CAGGGTACGA 
GAACTGGAGA ATGAGCTGGA TAATGAACAG AAACATGGTG TTGAGGCTGT GAAAGGTGTT 
CGTAAATATG AGAGGAGAGT GAAGGAACTC ACATACCAGA CTGAGGAAGA TAAGAAGAAC 
ATTTTGAGAC TGCAGGACCT GGTAGATAAA CTTCAGTCTA AGGTTAAAGC CTACAAGAGG 
CAGGCTGAAG AAGCTGAGGA ACAAGCCAAC ACCCATTTGG GCCGCTTCCG TAAGGTACAG 
CACGAGCTTG AAGAAGCAGA AGAAAGAGCC GATATTGCCG AGTCCCAAGT GAATAAGCTC 
AGGACAAAGA GCCGTGATAT TGGAGGCAAG AAAGAGTCTG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myh4 ( NM_001001244.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001001244.1 Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), mRNA.

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CloneID	OXa00939
Clone ID Related Accession (Same CDS sequence)	NM_001001244.1 , XM_031890312.1
Accession Version	NM_001001244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin, heavy chain 4, skeletal muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067305.1. ##Evidence-Data-START## Transcript exon combination :: BC067305.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028293, SAMD00028294 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGATG GAGAAATGTC CATCTTCGGC GAGGCCGCAC AATTTCTCAG AAAAAGTGAG 
AAAGAGCGTT TGGAGGCTCA GAGCAAGCCT TTTGATGCCA AAAACACTGT ATTTGTAGAT 
GATGTTAAGG AACTCTATGT CAAAGGTATG GTTACAGCCA GGGAAGATGG CAAAATAACT 
GTGAAGACCG ATGATGGAAG AACAGTAACC GTCAAAGAGA ACCAAATTTA CCCCCAGAAC 
CCTCCCAAGT TTGATAAAAT TGAAGACATG GCTATGATGA CCCATTTGAA TGAGGCTTCT 
GTCCTGTATA ACCTGAAAGA GCGTTATGCT GCATGGATGA TCTATACCTA CTCTGGCCTT 
TTCTGCGCCA CTGTTAACCC CTACAAGTGG CTGCCAGTGT ACAACCCTGA AGTTGTTGCC 
GGTTACAGAG GGAAAAAGCG TATGGAAGCT CCCCCTCACA TTTTCTCCCT TTCTGATAAT 
GCCTATCAGG CCATGTTAAC AGATCGTGAA AACCAGTCTG TCCTTATTAC TGGTGAATCT 
GGTGCAGGAA AGACTGTGAA CACCAAGCGT GTCATCCAGT ATTTTGCAAC AATTGCAGCC 
CTTGGAGACA AGAAGAAGGA ATCCTCCAAC AGCTTGCAGG GAAATCTGGA AGATCAGATC 
ATTCAGGCCA ACCCTCTGCT GGAAGCCTTT GGTAATGCCA AGACTGTGAG AAATGACAAC 
TCCTCTCGTT TTGGTAAATT CATCAGAATC CATTTTGGAA CTACAGGAAA ACTGTCTTCA 
GCTGATATTG AAACATATCT GTTGGAAAAG TCCAGAGTTA CTTTCCAGCT TTCAGCAGAA 
AGAAGCTATC ACATCTTCTA TCAGATCTTA ACAAATAAGA AGCCAGAAAT TGTTGAGATG 
CTTCTACTCA CTACTAACCC ATATGACTAC CCATCCATCA GTCAGGGTGA GCTTGTTGTG 
AAGAGCATTA ATGATGAAGA GGAGTTGATG GCAACAGATA GTGCCATTGA CGTTTTGGGT 
TTCAATCAAG AAGAAAAGAT GGGTATTTAC AAAATGACTG GTGCTGTCAT GCATCATGGC 
AACTTGAAGT TCAAGCAAAA GCAAAGAGAG GAGCAGGCTG AGCCTGATAG CACTGAGGTG 
GCTGACAAAA TTGCTTATCT GTTGGGCCTC AACTCTGCGG ATTTGCTCAA AGGTTTGTGC 
TACCCAAGAG TCAAGGTCGG CAATGAATTT GTTACCAAAG GACAGACTGT ACCACAGGTC 
TATAACTCTG TTGGTGCCCT GTGCAAGTCT GTTTTTGAAA AGCTGTTCTT GTGGATGGTC 
ACTCGTATTA ACCAACAGCT GGATACTAAG CAACCAAGAC AGTTCTTCAT TGGTGTGCTG 
GACATTGCTG GATTTGAAAT CTTTGATTTT AACAGCTTGG AGCAGCTCTG CATCAACTTT 
ACCAATGAAA AACTGCAACA GTTTTTCAAT CACCACATGT TCGTCCTGGA GCAGGAGGAA 
TACAAGAAGG AAGGAATTGA TTGGGAGTTT ATTGACTTCG GTATGGATTT GGCTGCCTGC 
ATTGAGCTTA TTGAGAAGCC ATTGGGTATC TTCTCCATCC TTGAAGAGGA GTGCATGTTC 
CCCAAAGCCA CTGATACTTC CTTCAAGAAC AAGCTATATG AGCAACATCT GGGCAAATGC 
AAGAACTTTG AGAAGCCCAA GCCTGGCAAA GGAAAGGCTG AAGCTCACTT CTCGCTTGTG 
CATTATGCTG GCACTGTGGA TTACAACATC AGTGGATGGC TTGAAAAGAA CAAGGACCCA 
CTGAACGAGT CAGTTGTCCA GCTCTACCAG AAGTCTTCTG TCAAACTGCT GTCCTTGCTC 
TACTCCAGCT ATGCTGCAAC TGACGGTGAT GCTGGTGGCA AAGGTGGTAA GAAAAAGAAG 
GGATCCTCTT TCCAGACTGT GTCTGGTCTC TTCAGGGAAA ATCTGAACAA ACTGATGACA 
AACTTGAGAA GCACTCACCC TCACTTTGTA CGTTGTTTGA TTCCCAATGA GACCAAGACT 
CCTGGTATCA TGGACAACCA TCTCCTTATT CACCAGCTGA GATGTAATGG TGTGCTGGAA 
GGTATTAGAA TCTGCAGGAA AGGATTCCCA AGCAGAATCC TCTATGGTGA TTTCAAACAA 
CGTTACAAAG TTCTGAATGC CAGTGCAATT CCAGAAGGGC AATTTATTGA CAGCAAGAAG 
GCTTGTGAGA AGCTTCTAGG CTCAATTGAT GTCGACCATA CTCAGTATAA ATTTGGACAC 
ACCAAGGTAT TTTTCAAAGC TGGTCTTTTG GGTACTTTGG AAGAAATGAG AGATGAAAAA 
TTGGCCCAAC TTATCACTCG CACCCAAGCT CTTTGCAGAG GATATTTGAT GAGGCTTGAG 
TTCACCAAAA TGATGGAAAG AAGGGAGGCC ATATATGTCA TCCAGTACAA TCTGAGGTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAACTGTACT TCAAGATCAA GCCTCTTCTG 
AAGAGCGCTG AGACTGAGAA AGAAATGGCA AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAAGCCCTCG TGAAGGCAGA AGCAAGGAAG AAGGAACTGG AGGAGAAAAT GGTTGCCATG 
CTCCAGGAAA AGAATGATCT AGTCCTGCAG GTTCAATCGG AAAGTGAAAC CTTGGCTGAT 
TCTGAGGAGA GATGTGAAGG GCTCATCAAA GTCAAAATTC AGCTGGAGTC TAAAATAAAG 
GAATTAACAG AAAGACTTGA AGATGAAGAA GAAAGCAATG CAGAGCTAAC AGCAAAGAAG 
AGAAAATTGG AGGATGAATG TTCTGAGCTC AAGAAAGACA TTGACGACCT TGAACTTACT 
TTGGCTAAAG TAGAAAAGGA GAAGCATGCC ACAGAAAACA AGGTTAAAAA CCTTACTGAA 
GAAATGGCAG TTCTCGATGA GAACATTTCC AAACTGTCCA AAGAAAAGAA GGCCCTTCAA 
GAGGCTCACC AACAAACACT TGATGACCTG CAAGCTGAAG AGGACAAAGT CAGTTCTCTG 
TCAAAAGCAA AGACAAAGCT GGAACAGCAA GTAGATGATC TGGAAGGTTC CCTGGAACAA 
GAGAAGAAAC TTCGTCTTGA CCTTGAGAGA GCCAAGAGAA AGCTTGAAGG AGATTTGAAA 
CTGACCCAGG AAACCGTCAT GGACCTTGAG AATGATAAAC AGCAAACTGA AGAAAAACTT 
AAAAAGAAAG ACTTTGAAAT AAGCCAGCTT CAAGGAAAGA TAGAGGATGA ACAGTCCCTG 
GGTACTCAGT TGCAGAAAAA GATCAAGGAA CTGCAGGCTC GAATTGAGGA AGTTGAGGAA 
GAAATTGAAG CTGAACGTGC AGCTCGTGCA AAGGTAGAGA AACAAAGGGC TGATCTTTCC 
AGGGAACTTG AGGAGATCAG TGAGAGACTT GAGGAAGCTG GAGGTGCAAC ATCTGCTCAG 
ATTGAGATGA ACAAAAAACG TGAAGCTGAG TTCCAGAAAC TGAGACGTGA CCTGGAGGAG 
GCAACCCTTC AGCATGAAGC CACTGCTGGT GCCCTGCGCA AAAAACATGC TGACAGTGTA 
GCTGAGCTTG GGGAGCAAAT TGATAACCTG CAGAGGGTTA AACAAAAGCT GGAGAAAGAA 
AAAAGTGAGC TGAAGATGGA GATTGATGAT CTTGCCAGCA ACTTGGAAAA TGTATCCAAA 
TCAAAGGCTA ATCTTGAAAA GGTTAACCGT GTGATTGAGG ACCAACTTAG TGAAGTGAAG 
GCAAAGGATG ATGAACATCA GCGTCTCCTC AACGACCTCA GTACTCAAAA GGCTCGCTTG 
CAGACTGAAA CCGGTGAGCT CTCCCGCCAG CTTGAAGAGA GGGAATCTCT GATTTCTCAG 
CTCTCAAGAG GAAAGCAGGG ATTTACCCAG CAAGTTGAAG AACTCAAGAG GCAGCTTGAA 
GAAGAAACAA AGGCTAAGAA TGCCCTTGCT CATGCTCTGC AATCAGCTCG TCATGACTGT 
GACTTGCTTA GAGAACAATT TGAGGAAGAA CAGGAGGCCA AGGCTGAACT TCAGCGCTCA 
CTGTCCAAAG CCAATGGTGA AGTTTCACAA TGGAGGACCA AATATGAAAC TGATGCAATT 
CAGCGTACTG AGGAACTTGA AGAAGCCAAG AAGAAACTTG CTCAGCGTTT ACAGGACGCT 
GAGGAGCAAG TTGAGGCTGT GAATTCCAAA TGCGCCTCCT TGGAAAAGAC TAAGCAGAGA 
CTGCAATCCG AAGTGGAGGA CTTGATGGTT GATGTTGAGA GAGCAAATAG TGCAGCAGCT 
GCTCTTGATA AGAAGCAGAG AAACTTTGAT AAGGTCCTGG TAGAATGGAA ACAGAAGTAT 
GAAGAGGGTC AGGCTGAATT GGAAGCAGCT CTTAAGGAAT CTCGCAGCCT GAGCACTGAG 
ATCTTCAAGA TGAAGAATGC TTATGAGGAA GCTCTGGAGC ATGTTGAAAC TCTGAAACGT 
GAAAACAAGA ACCTTCAACA GGAGATTTCA GATCTGACTG AACAGATTGG TGAGAGTGGA 
AAATCAGTTG TTGAATTGGA AAAGGCCAAG AAGCAGGTGG AACAAGAGAA GAATGATCTC 
CAAGCTGCTC TAGAAGAAGC AGAGGGATCT TTGGAACATG AAGAAGCCAA GATTCTGCGC 
ATCCAGCTTG AACTGAACCA GGTCAAATCT GAAGTGGACA GGAAAATTGC AGAGAAGGAT 
GAGGAAATTG AGCAACTGAA GAGAAACAGC CAGAGGTCCA TTGACACCAT GCAGAGCACA 
CTGGACTCTG AAATCAGGAG CAGAAATGAT GCACTGAGAC TGAAGAAGAA GATGGAAGGT 
GATCTGAATG AGCTTGAGAT CCAGCTGGGT CATGCCAACC GTCAGGCTTC TGAAGCACAG 
AAACAGCTTA GGAATGTACA GGGACAGTTC AAGGAGACCC AGCTCCAGCT GGATGAAGCA 
ATCAGGGCAC AGGAAGACCT GAAGGAACAG GTTGCTGTGA CTGAGCGCAG AAATAACTTG 
TTTCAAGCTG AAATTGAAGA AAACAGAGCT GGTCTGGAAC AGACAGAAAG ATCTCGTAAG 
ATTGCAGAAC AGGAGCTCCT AGATGCAAGT GAACGTCTTC AGCTGTTGCA TTCCCAGAAC 
ACAAGTCTCA TTAACAGCAA GAAGAAGCTT GAAAGTGACA TCTGTCAACT ACAGAATGAG 
GCCGAGGAAG CTGTCCAAGA AGCCAGAAAT GCTGAAGAGA AGGCAAAGAA GGCCATAACA 
GATGCTGCCC TGATGGCAGA AGAGTTGAAG AAGGAGCAGG ACACCAGTGC TCACTTAGAA 
AGGATGAAGA AGAACCTTGA ACAGACTGTG AAGGACCTGC AGCATCGCCT TGATGAAGCT 
GAACAGCTGG CAATGAAGGG TGGCAAGAAG CAGCTCCAGA AACTGGAATC CAGGGTACGA 
GAACTGGAGA ATGAGCTGGA TAATGAACAG AAACATGGTG TTGAGGCTGT GAAAGGTGTT 
CGTAAATATG AGAGGAGAGT GAAGGAACTC ACATACCAGA CTGAGGAAGA TAAGAAGAAC 
ATTTTGAGAC TGCAGGACCT GGTAGATAAA CTTCAGTCTA AGGTTAAAGC CTACAAGAGG 
CAGGCTGAAG AAGCTGAGGA ACAAGCCAAC ACCCATTTGG GCCGCTTCCG TAAGGTACAG 
CACGAGCTTG AAGAAGCAGA AGAAAGAGCC GATATTGCCG AGTCCCAAGT GAATAAGCTC 
AGGACAAAGA GCCGTGATAT TGGAGGCAAG AAAGAGTCTG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001244.1
CDS	46..5853
Translation	MGDGEMSIFGEAAQFLRKSEKERLEAQSKPFDAKNTVFVDDVKELYVKGMVTAREDGKITVKTDDGRTVTVKENQIYPQNPPKFDKIEDMAMMTHLNEASVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYNPEVVAGYRGKKRMEAPPHIFSLSDNAYQAMLTDRENQSVLITGESGAGKTVNTKRVIQYFATIAALGDKKKESSNSLQGNLEDQIIQANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLSSADIETYLLEKSRVTFQLSAERSYHIFYQILTNKKPEIVEMLLLTTNPYDYPSISQGELVVKSINDEEELMATDSAIDVLGFNQEEKMGIYKMTGAVMHHGNLKFKQKQREEQAEPDSTEVADKIAYLLGLNSADLLKGLCYPRVKVGNEFVTKGQTVPQVYNSVGALCKSVFEKLFLWMVTRINQQLDTKQPRQFFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYEQHLGKCKNFEKPKPGKGKAEAHFSLVHYAGTVDYNISGWLEKNKDPLNESVVQLYQKSSVKLLSLLYSSYAATDGDAGGKGGKKKKGSSFQTVSGLFRENLNKLMTNLRSTHPHFVRCLIPNETKTPGIMDNHLLIHQLRCNGVLEGIRICRKGFPSRILYGDFKQRYKVLNASAIPEGQFIDSKKACEKLLGSIDVDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQALCRGYLMRLEFTKMMERREAIYVIQYNLRSFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEALVKAEARKKELEEKMVAMLQEKNDLVLQVQSESETLADSEERCEGLIKVKIQLESKIKELTERLEDEEESNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAVLDENISKLSKEKKALQEAHQQTLDDLQAEEDKVSSLSKAKTKLEQQVDDLEGSLEQEKKLRLDLERAKRKLEGDLKLTQETVMDLENDKQQTEEKLKKKDFEISQLQGKIEDEQSLGTQLQKKIKELQARIEEVEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKLRRDLEEATLQHEATAGALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNLENVSKSKANLEKVNRVIEDQLSEVKAKDDEHQRLLNDLSTQKARLQTETGELSRQLEERESLISQLSRGKQGFTQQVEELKRQLEEETKAKNALAHALQSARHDCDLLREQFEEEQEAKAELQRSLSKANGEVSQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEQVEAVNSKCASLEKTKQRLQSEVEDLMVDVERANSAAAALDKKQRNFDKVLVEWKQKYEEGQAELEAALKESRSLSTEIFKMKNAYEEALEHVETLKRENKNLQQEISDLTEQIGESGKSVVELEKAKKQVEQEKNDLQAALEEAEGSLEHEEAKILRIQLELNQVKSEVDRKIAEKDEEIEQLKRNSQRSIDTMQSTLDSEIRSRNDALRLKKKMEGDLNELEIQLGHANRQASEAQKQLRNVQGQFKETQLQLDEAIRAQEDLKEQVAVTERRNNLFQAEIEENRAGLEQTERSRKIAEQELLDASERLQLLHSQNTSLINSKKKLESDICQLQNEAEEAVQEARNAEEKAKKAITDAALMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLAMKGGKKQLQKLESRVRELENELDNEQKHGVEAVKGVRKYERRVKELTYQTEEDKKNILRLQDLVDKLQSKVKAYKRQAEEAEEQANTHLGRFRKVQHELEEAEERADIAESQVNKLRTKSRDIGGKKESEE

Target ORF information:

RefSeq Version	NM_001001244.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin, heavy chain 4, skeletal muscle (myh4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001244.1

ORF Insert Sequence:

ATGGGTGATG GAGAAATGTC CATCTTCGGC GAGGCCGCAC AATTTCTCAG AAAAAGTGAG 
AAAGAGCGTT TGGAGGCTCA GAGCAAGCCT TTTGATGCCA AAAACACTGT ATTTGTAGAT 
GATGTTAAGG AACTCTATGT CAAAGGTATG GTTACAGCCA GGGAAGATGG CAAAATAACT 
GTGAAGACCG ATGATGGAAG AACAGTAACC GTCAAAGAGA ACCAAATTTA CCCCCAGAAC 
CCTCCCAAGT TTGATAAAAT TGAAGACATG GCTATGATGA CCCATTTGAA TGAGGCTTCT 
GTCCTGTATA ACCTGAAAGA GCGTTATGCT GCATGGATGA TCTATACCTA CTCTGGCCTT 
TTCTGCGCCA CTGTTAACCC CTACAAGTGG CTGCCAGTGT ACAACCCTGA AGTTGTTGCC 
GGTTACAGAG GGAAAAAGCG TATGGAAGCT CCCCCTCACA TTTTCTCCCT TTCTGATAAT 
GCCTATCAGG CCATGTTAAC AGATCGTGAA AACCAGTCTG TCCTTATTAC TGGTGAATCT 
GGTGCAGGAA AGACTGTGAA CACCAAGCGT GTCATCCAGT ATTTTGCAAC AATTGCAGCC 
CTTGGAGACA AGAAGAAGGA ATCCTCCAAC AGCTTGCAGG GAAATCTGGA AGATCAGATC 
ATTCAGGCCA ACCCTCTGCT GGAAGCCTTT GGTAATGCCA AGACTGTGAG AAATGACAAC 
TCCTCTCGTT TTGGTAAATT CATCAGAATC CATTTTGGAA CTACAGGAAA ACTGTCTTCA 
GCTGATATTG AAACATATCT GTTGGAAAAG TCCAGAGTTA CTTTCCAGCT TTCAGCAGAA 
AGAAGCTATC ACATCTTCTA TCAGATCTTA ACAAATAAGA AGCCAGAAAT TGTTGAGATG 
CTTCTACTCA CTACTAACCC ATATGACTAC CCATCCATCA GTCAGGGTGA GCTTGTTGTG 
AAGAGCATTA ATGATGAAGA GGAGTTGATG GCAACAGATA GTGCCATTGA CGTTTTGGGT 
TTCAATCAAG AAGAAAAGAT GGGTATTTAC AAAATGACTG GTGCTGTCAT GCATCATGGC 
AACTTGAAGT TCAAGCAAAA GCAAAGAGAG GAGCAGGCTG AGCCTGATAG CACTGAGGTG 
GCTGACAAAA TTGCTTATCT GTTGGGCCTC AACTCTGCGG ATTTGCTCAA AGGTTTGTGC 
TACCCAAGAG TCAAGGTCGG CAATGAATTT GTTACCAAAG GACAGACTGT ACCACAGGTC 
TATAACTCTG TTGGTGCCCT GTGCAAGTCT GTTTTTGAAA AGCTGTTCTT GTGGATGGTC 
ACTCGTATTA ACCAACAGCT GGATACTAAG CAACCAAGAC AGTTCTTCAT TGGTGTGCTG 
GACATTGCTG GATTTGAAAT CTTTGATTTT AACAGCTTGG AGCAGCTCTG CATCAACTTT 
ACCAATGAAA AACTGCAACA GTTTTTCAAT CACCACATGT TCGTCCTGGA GCAGGAGGAA 
TACAAGAAGG AAGGAATTGA TTGGGAGTTT ATTGACTTCG GTATGGATTT GGCTGCCTGC 
ATTGAGCTTA TTGAGAAGCC ATTGGGTATC TTCTCCATCC TTGAAGAGGA GTGCATGTTC 
CCCAAAGCCA CTGATACTTC CTTCAAGAAC AAGCTATATG AGCAACATCT GGGCAAATGC 
AAGAACTTTG AGAAGCCCAA GCCTGGCAAA GGAAAGGCTG AAGCTCACTT CTCGCTTGTG 
CATTATGCTG GCACTGTGGA TTACAACATC AGTGGATGGC TTGAAAAGAA CAAGGACCCA 
CTGAACGAGT CAGTTGTCCA GCTCTACCAG AAGTCTTCTG TCAAACTGCT GTCCTTGCTC 
TACTCCAGCT ATGCTGCAAC TGACGGTGAT GCTGGTGGCA AAGGTGGTAA GAAAAAGAAG 
GGATCCTCTT TCCAGACTGT GTCTGGTCTC TTCAGGGAAA ATCTGAACAA ACTGATGACA 
AACTTGAGAA GCACTCACCC TCACTTTGTA CGTTGTTTGA TTCCCAATGA GACCAAGACT 
CCTGGTATCA TGGACAACCA TCTCCTTATT CACCAGCTGA GATGTAATGG TGTGCTGGAA 
GGTATTAGAA TCTGCAGGAA AGGATTCCCA AGCAGAATCC TCTATGGTGA TTTCAAACAA 
CGTTACAAAG TTCTGAATGC CAGTGCAATT CCAGAAGGGC AATTTATTGA CAGCAAGAAG 
GCTTGTGAGA AGCTTCTAGG CTCAATTGAT GTCGACCATA CTCAGTATAA ATTTGGACAC 
ACCAAGGTAT TTTTCAAAGC TGGTCTTTTG GGTACTTTGG AAGAAATGAG AGATGAAAAA 
TTGGCCCAAC TTATCACTCG CACCCAAGCT CTTTGCAGAG GATATTTGAT GAGGCTTGAG 
TTCACCAAAA TGATGGAAAG AAGGGAGGCC ATATATGTCA TCCAGTACAA TCTGAGGTCA 
TTCATGAATG TCAAACACTG GCCATGGATG AAACTGTACT TCAAGATCAA GCCTCTTCTG 
AAGAGCGCTG AGACTGAGAA AGAAATGGCA AACATGAAGG AAGAGTTTGA GAAGACCAAG 
GAAGCCCTCG TGAAGGCAGA AGCAAGGAAG AAGGAACTGG AGGAGAAAAT GGTTGCCATG 
CTCCAGGAAA AGAATGATCT AGTCCTGCAG GTTCAATCGG AAAGTGAAAC CTTGGCTGAT 
TCTGAGGAGA GATGTGAAGG GCTCATCAAA GTCAAAATTC AGCTGGAGTC TAAAATAAAG 
GAATTAACAG AAAGACTTGA AGATGAAGAA GAAAGCAATG CAGAGCTAAC AGCAAAGAAG 
AGAAAATTGG AGGATGAATG TTCTGAGCTC AAGAAAGACA TTGACGACCT TGAACTTACT 
TTGGCTAAAG TAGAAAAGGA GAAGCATGCC ACAGAAAACA AGGTTAAAAA CCTTACTGAA 
GAAATGGCAG TTCTCGATGA GAACATTTCC AAACTGTCCA AAGAAAAGAA GGCCCTTCAA 
GAGGCTCACC AACAAACACT TGATGACCTG CAAGCTGAAG AGGACAAAGT CAGTTCTCTG 
TCAAAAGCAA AGACAAAGCT GGAACAGCAA GTAGATGATC TGGAAGGTTC CCTGGAACAA 
GAGAAGAAAC TTCGTCTTGA CCTTGAGAGA GCCAAGAGAA AGCTTGAAGG AGATTTGAAA 
CTGACCCAGG AAACCGTCAT GGACCTTGAG AATGATAAAC AGCAAACTGA AGAAAAACTT 
AAAAAGAAAG ACTTTGAAAT AAGCCAGCTT CAAGGAAAGA TAGAGGATGA ACAGTCCCTG 
GGTACTCAGT TGCAGAAAAA GATCAAGGAA CTGCAGGCTC GAATTGAGGA AGTTGAGGAA 
GAAATTGAAG CTGAACGTGC AGCTCGTGCA AAGGTAGAGA AACAAAGGGC TGATCTTTCC 
AGGGAACTTG AGGAGATCAG TGAGAGACTT GAGGAAGCTG GAGGTGCAAC ATCTGCTCAG 
ATTGAGATGA ACAAAAAACG TGAAGCTGAG TTCCAGAAAC TGAGACGTGA CCTGGAGGAG 
GCAACCCTTC AGCATGAAGC CACTGCTGGT GCCCTGCGCA AAAAACATGC TGACAGTGTA 
GCTGAGCTTG GGGAGCAAAT TGATAACCTG CAGAGGGTTA AACAAAAGCT GGAGAAAGAA 
AAAAGTGAGC TGAAGATGGA GATTGATGAT CTTGCCAGCA ACTTGGAAAA TGTATCCAAA 
TCAAAGGCTA ATCTTGAAAA GGTTAACCGT GTGATTGAGG ACCAACTTAG TGAAGTGAAG 
GCAAAGGATG ATGAACATCA GCGTCTCCTC AACGACCTCA GTACTCAAAA GGCTCGCTTG 
CAGACTGAAA CCGGTGAGCT CTCCCGCCAG CTTGAAGAGA GGGAATCTCT GATTTCTCAG 
CTCTCAAGAG GAAAGCAGGG ATTTACCCAG CAAGTTGAAG AACTCAAGAG GCAGCTTGAA 
GAAGAAACAA AGGCTAAGAA TGCCCTTGCT CATGCTCTGC AATCAGCTCG TCATGACTGT 
GACTTGCTTA GAGAACAATT TGAGGAAGAA CAGGAGGCCA AGGCTGAACT TCAGCGCTCA 
CTGTCCAAAG CCAATGGTGA AGTTTCACAA TGGAGGACCA AATATGAAAC TGATGCAATT 
CAGCGTACTG AGGAACTTGA AGAAGCCAAG AAGAAACTTG CTCAGCGTTT ACAGGACGCT 
GAGGAGCAAG TTGAGGCTGT GAATTCCAAA TGCGCCTCCT TGGAAAAGAC TAAGCAGAGA 
CTGCAATCCG AAGTGGAGGA CTTGATGGTT GATGTTGAGA GAGCAAATAG TGCAGCAGCT 
GCTCTTGATA AGAAGCAGAG AAACTTTGAT AAGGTCCTGG TAGAATGGAA ACAGAAGTAT 
GAAGAGGGTC AGGCTGAATT GGAAGCAGCT CTTAAGGAAT CTCGCAGCCT GAGCACTGAG 
ATCTTCAAGA TGAAGAATGC TTATGAGGAA GCTCTGGAGC ATGTTGAAAC TCTGAAACGT 
GAAAACAAGA ACCTTCAACA GGAGATTTCA GATCTGACTG AACAGATTGG TGAGAGTGGA 
AAATCAGTTG TTGAATTGGA AAAGGCCAAG AAGCAGGTGG AACAAGAGAA GAATGATCTC 
CAAGCTGCTC TAGAAGAAGC AGAGGGATCT TTGGAACATG AAGAAGCCAA GATTCTGCGC 
ATCCAGCTTG AACTGAACCA GGTCAAATCT GAAGTGGACA GGAAAATTGC AGAGAAGGAT 
GAGGAAATTG AGCAACTGAA GAGAAACAGC CAGAGGTCCA TTGACACCAT GCAGAGCACA 
CTGGACTCTG AAATCAGGAG CAGAAATGAT GCACTGAGAC TGAAGAAGAA GATGGAAGGT 
GATCTGAATG AGCTTGAGAT CCAGCTGGGT CATGCCAACC GTCAGGCTTC TGAAGCACAG 
AAACAGCTTA GGAATGTACA GGGACAGTTC AAGGAGACCC AGCTCCAGCT GGATGAAGCA 
ATCAGGGCAC AGGAAGACCT GAAGGAACAG GTTGCTGTGA CTGAGCGCAG AAATAACTTG 
TTTCAAGCTG AAATTGAAGA AAACAGAGCT GGTCTGGAAC AGACAGAAAG ATCTCGTAAG 
ATTGCAGAAC AGGAGCTCCT AGATGCAAGT GAACGTCTTC AGCTGTTGCA TTCCCAGAAC 
ACAAGTCTCA TTAACAGCAA GAAGAAGCTT GAAAGTGACA TCTGTCAACT ACAGAATGAG 
GCCGAGGAAG CTGTCCAAGA AGCCAGAAAT GCTGAAGAGA AGGCAAAGAA GGCCATAACA 
GATGCTGCCC TGATGGCAGA AGAGTTGAAG AAGGAGCAGG ACACCAGTGC TCACTTAGAA 
AGGATGAAGA AGAACCTTGA ACAGACTGTG AAGGACCTGC AGCATCGCCT TGATGAAGCT 
GAACAGCTGG CAATGAAGGG TGGCAAGAAG CAGCTCCAGA AACTGGAATC CAGGGTACGA 
GAACTGGAGA ATGAGCTGGA TAATGAACAG AAACATGGTG TTGAGGCTGT GAAAGGTGTT 
CGTAAATATG AGAGGAGAGT GAAGGAACTC ACATACCAGA CTGAGGAAGA TAAGAAGAAC 
ATTTTGAGAC TGCAGGACCT GGTAGATAAA CTTCAGTCTA AGGTTAAAGC CTACAAGAGG 
CAGGCTGAAG AAGCTGAGGA ACAAGCCAAC ACCCATTTGG GCCGCTTCCG TAAGGTACAG 
CACGAGCTTG AAGAAGCAGA AGAAAGAGCC GATATTGCCG AGTCCCAAGT GAATAAGCTC 
AGGACAAAGA GCCGTGATAT TGGAGGCAAG AAAGAGTCTG AAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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myh4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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