Myh1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh1
Entrez Gene ID	17879
Full Name	myosin, heavy polypeptide 1, skeletal muscle, adult
Synonyms	A530084A17Rik,IId,IId/x,MHC-2X/D,MHC2X/D,MYHC-IIX,MdMs,MyHC-IId/x,MyHC-IIx/d,Myhs-f,Myhs-f2,Myhsf2
General protein information	Preferred Names myosin, heavy polypeptide 1, skeletal muscle, adult Names myosin-1 myHC-2x myosin heavy chain 1 myosin heavy chain 2X myosin heavy chain, skeletal muscle, adult 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B3\|11 40.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017314318.1	XP_017169807.1	myosin-1 isoform X1
XM_017314318.2	XP_017169807.1	myosin-1 isoform X1
NM_030679.1	NP_109604.1	myosin-1
NM_030679.2	NP_109604.1	myosin-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH1	NP_005954.3
Xenopus tropicalis (tropical clawed frog)	myh8	XP_004916771.1
Xenopus tropicalis (tropical clawed frog)	LOC100495802	XP_002935255.1
Bos taurus (cattle)	MYH1	NP_776542.1
Xenopus tropicalis (tropical clawed frog)	myh4	NP_001001244.1
Xenopus tropicalis (tropical clawed frog)	myh2	NP_001006805.1
Xenopus tropicalis (tropical clawed frog)	LOC100495968	XP_002935256.2
Pan troglodytes (chimpanzee)	MYH1	XP_003953177.1
Canis lupus familiaris (dog)	MYH1	NP_001107189.1
Mus musculus (house mouse)	Myh1	NP_109604.1
Rattus norvegicus (Norway rat)	Myh1	NP_001128630.1
Xenopus tropicalis (tropical clawed frog)	LOC100495638	XP_002935254.1

Gene Ontology

The following Myh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20243	XM_017314318.2 Latest version!	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu20243	NM_030679.2 Latest version!	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu20243	XM_017314318.1	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu20243	NM_030679.1	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh1 ( XM_017314318.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169807.1 Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.

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CloneID	OMu20243
Clone ID Related Accession (Same CDS sequence)	XM_017314318.1 , XM_017314318.2 , NM_030679.1 , NM_030679.2
Accession Version	XM_017314318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	myosin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169807.1
CDS	86..5914
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTVTVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAAAEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQIEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	XM_017314318.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314318.2

ORF Insert Sequence:

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh1 ( NM_030679.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109604.1 Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.

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CloneID	OMu20243
Clone ID Related Accession (Same CDS sequence)	XM_017314318.1 , XM_017314318.2 , NM_030679.1 , NM_030679.2
Accession Version	NM_030679.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-16
Organism	Mus musculus(house mouse)
Product	myosin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC108329.1. On or before Dec 7, 2005 this sequence version replaced XM_354615.4, XM_908205.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109604.1
CDS	85..5913
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTVTVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAAAEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQIEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	NM_030679.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030679.1

ORF Insert Sequence:

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh1 ( NM_030679.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109604.1 Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.

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CloneID	OMu20243
Clone ID Related Accession (Same CDS sequence)	XM_017314318.1 , XM_017314318.2 , NM_030679.1 , NM_030679.2
Accession Version	NM_030679.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	myosin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK041122.1 and BC108329.1. On Feb 16, 2018 this sequence version replaced NM_030679.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108329.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109604.1
CDS	97..5925
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTVTVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAAAEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQIEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	NM_030679.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030679.2

ORF Insert Sequence:

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh1 ( XM_017314318.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169807.1 Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.

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CloneID	OMu20243
Clone ID Related Accession (Same CDS sequence)	XM_017314318.1 , XM_017314318.2 , NM_030679.1 , NM_030679.2
Accession Version	XM_017314318.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	myosin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169807.1
CDS	86..5914
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESFVKATVQSREGGKVTAKTEGGTTVTVKDDQVYPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIDILGFTSDERVSIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLQNLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKVEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSSMKTLAYLFSGAAAAAEAESGGGGGKKGAKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQAMCRGYLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKAKENLAKAEAKRKELEEKMVALMQEKNDLQLQVQSEADSLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKIRMDLERAKRKLEGDLKLAQESTMDVENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQVSELKTKEEEQQRLINELTAQRGRLQTESGEYSRQLDEKDSLVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSSRHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILAEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQIEQEKSELQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRVVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHSNRMAAEALRNYRNTQGILKDTQLHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVENEQKRNVEAIKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQSKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	XM_017314318.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin, heavy polypeptide 1, skeletal muscle, adult (Myh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314318.1

ORF Insert Sequence:

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTC GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGACG CCAAGTCATC GGTGTTTGTG 
GTGGATGCTA AGGAGTCTTT TGTAAAAGCA ACGGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACAGCCAAGA CCGAAGGCGG AACTACTGTA ACAGTAAAAG ATGATCAGGT CTACCCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCATGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC CTACTCGGGC 
CTGTTCTGCG TCACTGTCAA CCCCTACAAG TGGCTGCCAG TGTATAACGC AGAGGTGGTG 
GCAGCCTACA GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AATGCCTACC AGTTCATGCT GACAGATCGG GAGAACCAGT CTATTTTGAT CACCGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAAAAGAA GGAGGAGGCA ACTTCTGGCA AAATGCAGGG GACGCTGGAG 
GACCAAATCA TCAGCGCCAA CCCCCTGCTG GAGGCCTTTG GGAACGCCAA GACCGTGAGG 
AATGACAACT CTTCCCGCTT TGGTAAGTTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTA 
AAAGCTGAGA GAAGCTACCA CATTTTTTAC CAGATTATGT CTAACAAGAA GCCGGATCTA 
ATCGAAATGC TCCTGATCAC CACCAACCCA TACGACTACG CCTTTGTCAG TCAGGGGGAG 
ATCACGGTGC CCAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAT 
ATTCTGGGCT TCACTTCTGA TGAAAGAGTC TCCATCTATA AGCTCACAGG GGCTGTGATG 
CATTATGGGA ACATGAAATT CAAGCAGAAG CAACGAGAGG AGCAGGCTGA GCCAGACGGC 
ACCGAAGTTG CTGACAAGGC TGCCTATCTC CAAAATCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTGTGTT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTG 
CAGCAGGTGT ACAACTCAGT AGGCGCCCTA GCCAAAGCCG TCTATGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCCGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTCTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA GGGCATCGAG TGGGAGTTCA TTGACTTTGG GATGGACCTG 
GCGGCCTGCA TCGAGCTCAT CGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCGAC AGACACCTCC TTCAAGAACA AGCTGTATGA GCAGCATCTT 
GGAAAGTCCA ACAACTTCCA AAAGCCCAAA CCTGCCAAAG GCAAGGTTGA AGCTCACTTC 
TCCCTGGTGC ACTATGCGGG CACTGTGGAC TACAACATCG CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC TGTGGTGGGG CTGTACCAGA AGTCTTCAAT GAAAACTCTG 
GCTTACCTCT TCTCTGGGGC AGCAGCAGCT GCGGAAGCAG AGTCTGGTGG CGGTGGTGGA 
AAGAAAGGGG CCAAGAAGAA GGGTTCCTCC TTCCAGACCG TGTCAGCCCT CTTCAGGGAG 
AATCTGAATA AGCTAATGAC CAATCTGAGG AGCACTCACC CCCACTTTGT ACGGTGCATC 
ATCCCCAATG AAACCAAGAC TCCTGGTGCC ATGGAGCACG AACTGGTCCT GCACCAGCTG 
AGGTGTAACG GGGTGCTGGA GGGCATCCGC ATCTGCAGGA AGGGCTTCCC CAGCAGGATC 
CTCTATGCAG ACTTCAAGCA GAGATATAAG GTTTTAAATG CAAGTGCCAT CCCTGAGGGA 
CAGTTCATCG ATAGCAAGAA GGCTTCTGAG AAGCTCCTGG GATCCATTGA TATTGACCAC 
ACTCAGTATA AGTTTGGTCA CACCAAGGTC TTTTTCAAAG CTGGTCTTCT GGGGCTCCTA 
GAGGAGATGA GAGATGACAA GTTGGCCCAG CTTATAACCC GGACCCAGGC TATGTGCAGA 
GGGTACTTGG CGAGAGTGGA GTACCAGAAG ATGGTGGAGA GAAGAGAGTC CATCTTCTGC 
ATCCAGTACA ACGTCCGCGC CTTCATGAAT GTGAAACACT GGCCCTGGAT GAAGCTGTAT 
TTCAAGATCA AACCTCTCTT GAAGAGCGCA GAGACCGAGA AGGAGATGGC CAACATGAAG 
GAAGAATTTG AAAAAGCTAA AGAAAACCTT GCAAAGGCGG AGGCCAAAAG AAAAGAGCTG 
GAAGAAAAGA TGGTAGCTCT GATGCAAGAG AAAAATGACC TGCAGCTCCA AGTTCAGTCT 
GAAGCAGACA GCTTGGCCGA TGCAGAGGAG CGATGCGACC AGCTGATCAA AACCAAAATC 
CAGCTGGAGG CCAAAATCAA AGAGGTGACC GAGAGGGCCG AGGATGAGGA GGAGATCAAC 
GCAGAGCTCA CGGCCAAGAA GAGGAAGCTG GAGGACGAGT GCTCAGAGCT GAAGAAGGAC 
ATCGATGACC TTGAGCTGAC ACTGGCCAAG GTTGAGAAGG AGAAGCACGC CACGGAGAAC 
AAGGTGAAAA ACCTGACAGA GGAGATGGCA GGCCTGGATG AAACCATCGC CAAGCTGACC 
AAGGAGAAGA AGGCCCTCCA GGAGGCCCAC CAGCAGACCC TGGATGACCT GCAGGCAGAG 
GAGGACAAAG TCAACACCCT GACCAAAGCC AAAATCAAGC TGGAACAGCA AGTGGATGAT 
CTTGAAGGCT CTCTGGAACA AGAAAAGAAA ATCCGGATGG ATCTTGAAAG AGCCAAGAGG 
AAACTGGAGG GAGACCTGAA ACTGGCCCAA GAGTCCACAA TGGACGTAGA AAACGATAAA 
CAGCAGCTTG ATGAGAAACT CAAAAAGAAA GAGTTTGAAA TGAGCAATCT GCAGAGCAAG 
ATTGAGGACG AGCAGGCCCT CGGCATGCAG CTGCAGAAGA AGATCAAGGA GTTGCAGGCC 
CGCATCGAGG AGCTGGAGGA GGAAATCGAG GCAGAGCGGG CCTCCAGGGC CAAAGCAGAG 
AAGCAGCGCT CTGACCTCTC CCGGGAACTG GAGGAGATCA GCGAGAGGCT GGAAGAAGCT 
GGCGGAGCCA CTTCAGCCCA AATCGAGATG AACAAGAAGA GAGAGGCTGA GTTCCAGAAA 
ATGCGCAGGG ACCTGGAGGA GGCCACGCTG CAGCATGAAG CCACAGCAGC CACCCTGAGG 
AAGAAGCACG CGGACAGCGT GGCTGAGCTC GGGGAGCAGA TCGACAACCT GCAGCGGGTG 
AAGCAGAAGC TGGAGAAGGA GAAGAGCGAG ATGAAGATGG AGATCGATGA CCTCGCTAGT 
AACATGGAGG TCATCTCCAA ATCTAAGGGA AACCTTGAGA AGATGTGCCG CACACTGGAG 
GACCAAGTGA GTGAGCTGAA GACCAAGGAG GAGGAACAGC AGCGGCTGAT CAATGAGCTG 
ACTGCGCAGA GGGGGCGTCT GCAGACGGAG TCAGGTGAAT ACTCACGCCA GCTAGACGAA 
AAGGATTCAT TAGTTTCCCA GCTCTCCAGG GGCAAACAGG CATTCACTCA ACAGATTGAG 
GAGCTGAAGA GGCAGCTTGA AGAGGAGATA AAGGCCAAGA GTGCCTTAGC TCATGCTCTT 
CAGTCCTCCC GCCATGACTG TGACCTGCTG CGGGAACAGT ATGAAGAAGA ACAGGAAGCC 
AAGGCTGAGC TGCAGAGGGC CATGTCCAAA GCCAACAGTG AAGTGGCCCA GTGGAGGACA 
AAATATGAGA CGGATGCCAT CCAGCGCACA GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCTCAGCGTC TGCAGGATGC TGAGGAGCAC GTAGAAGCTG TGAACGCCAA GTGCGCTTCC 
CTGGAGAAGA CGAAGCAGCG GCTGCAGAAC GAGGTGGAGG ACCTCATGAT TGATGTGGAG 
AGGACCAACG CAGCCTGCGC CGCCCTGGAC AAGAAGCAGA GAAACTTTGA CAAGATCCTG 
GCAGAATGGA AACAGAAGTA TGAGGAAACC CATGCTGAAC TTGAAGCCTC CCAGAAGGAG 
TCCCGCTCTC TCAGCACAGA GCTGTTCAAG ATTAAGAATG CTTATGAGGA ATCTTTAGAC 
CATCTCGAAA CCTTGAAAAG GGAGAATAAG AATTTGCAAC AGGAGATTTC TGACCTCACT 
GAACAGATTG CAGAAGGAGG GAAACGCATC CATGAACTGG AGAAAATAAA GAAGCAAATT 
GAGCAGGAAA AATCTGAGCT CCAGGCTGCT TTAGAGGAAG CAGAGGCATC TCTGGAGCAC 
GAGGAGGGAA AAATCCTGCG CATCCAGCTG GAGCTGAACC AAGTCAAGTC TGAGATCGAC 
AGGAAGATTG CTGAGAAGGA TGAGGAGATC GACCAGCTGA AGAGAAACCA CATTAGAGTC 
GTGGAGTCCA TGCAGAGCAC GCTGGATGCT GAGATTAGGA GCAGGAATGA CGCCATCAGG 
CTCAAGAAGA AGATGGAGGG AGACCTGAAC GAGATGGAAA TCCAGCTGAA CCACTCCAAC 
CGCATGGCTG CCGAGGCCCT GAGAAACTAT AGGAACACAC AAGGCATCCT CAAGGACACT 
CAGCTGCACC TGGACGATGC TCTCCGGGGC CAGGAGGACC TGAAGGAGCA GCTGGCCATG 
GTTGAGCGCA GAGCCAACCT GCTGCAGGCT GAGATCGAGG AGCTGCGGGC CACCCTGGAG 
CAGACGGAGA GGAGCAGGAA GATTGCAGAG CAGGAGTTGC TGGATGCCAG TGAGCGCGTG 
CAGCTCCTCC ACACCCAGAA CACCAGCCTC ATCAACACCA AGAAGAAGCT GGAGACAGAC 
ATTTCCCAAA TCCAGGGAGA GATGGAGGAC ATCGTCCAGG AAGCCCGCAA TGCAGAAGAG 
AAGGCCAAGA AAGCCATCAC TGATGCCGCC ATGATGGCGG AGGAGCTGAA GAAGGAGCAG 
GATACCAGCG CCCACCTGGA GCGGATGAAG AAGAACCTGG AGCAGACGGT GAAGGACCTG 
CAGCACCGTC TGGATGAGGC TGAGCAGCTG GCGCTGAAGG GCGGCAAGAA GCAGATCCAG 
AAACTGGAGG CCAGGGTCCG TGAACTTGAA GGAGAAGTGG AAAATGAACA GAAGCGCAAC 
GTGGAAGCTA TCAAGGGTCT GCGCAAACAT GAGAGAAGAG TGAAAGAACT CACTTATCAA 
ACTGAGGAAG ACCGCAAGAA TGTTCTCAGG CTCCAGGACC TTGTGGACAA ACTGCAATCA 
AAGGTCAAGG CCTACAAGAG ACAAGCTGAG GAAGCGGAGG AACAATCCAA TGTCAACCTG 
GCCAAATTCC GCAAGATCCA GCACGAGCTG GAGGAAGCCG AGGAGCGGGC TGACATCGCG 
GAGTCCCAGG TCAACAAGCT GCGGGTGAAG AGCCGGGAGG TTCACACCAA AATCATAAGC 
GAAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Myh1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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