BacPower^® Guaranteed Bacterial Recombinant Protein Expression Service

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Our BacPower^® Guaranteed Bacterial Recombinant Protein Expression Service offer a comprehensive panel of options for your target protein production in an E. coli system with guaranteed protein amounts, purities as well as endotoxin levels to meet a variety of your research needs, especially for protein antigen production and vaccine candidate screening. You simply provide protein sequence and we will deliver the protein at the amount and purity that you specified.

All BacPower^® Guaranteed Bacterial Recombinant Protein Expression Services start with our proprietary codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial protein expression system. Pure protein is delivered to you in as little as 4 weeks from project initiation. If your target recombinant protein becomes insoluble in the inclusion body fraction, FoldArt™, our proprietary protein refolding technology will be applied to solubilize the protein at no extra cost to you. In case we fail on either the amount or purity as specified, there is no charge to you whatsoever. Place your order today to experience unprecedented, high quality services yet competitive pricing, offered with our all of our Bacterial Protein Expression Services.

BacPower^® Guaranteed Bacterial Protein Expression Services (SC1253) with Unprecedented Assurances

• Gene synthesis is included in the package – no additional cost to you.
• Guarantees the amount of custom recombinant protein – no cost to you if project fails.
• Guarantees the purity of custom recombinant protein – no cost to you if desired purity is not achieved.
• Guarantee the no protein loss when bundling with endotoxin removal option.
• Competitive price - starting from $1600.
• Fast turnaround time - as little as 4 weeks.

Any combination of protein amount, purity and endotoxin level can be chosen from this list

Terms and Conditions

• Protein sequences are subject to pre-project analysis.
• Exclusions are applicable to membrane proteins, toxic proteins and for target proteins greater than 800 amino acids.
• To increase the expression level of your protein, codon optimization using our proprietary, OptimumGene™ technology, will be performed during gene synthesis.
• Insoluble proteins maybe subject to FoldArt™ technology, GenScript's proprietary protein refolding platform.
• In all cases, final protein will be guaranteed to meet customer specifications, failing which, any charge to the customer will be waived.

BacPower^® Guaranteed Bacterial Protein Expression (SC1253) Service Details

Steps	Specification	Timeline*	Deliverables
Gene synthesis, Cloning, Plasmid Prep	Gene synthesis and Codon optimization Sub cloning into appropriate expression vector Plasmid Prep and DNA QC	≤2 weeks	Protein in buffered solution with your specified amount & purity – Guaranteed Optimized gene sequence report QC data
Protein Expression Testing	Transform plasmids into appropriate bacterial expression strain Protein expression evaluation and optimization	≤1 week
Protein Purification, Tag Removal (Optional) & Refolding (Optional)	Scale up protein expression using optimized conditions Purification to reach desired protein amounts and purity Tag removal and separation of tag-free protein (if requested) Refolding in case of insoluble protein (performed only if required)	≤1 week
QC & Delivery	Purity analysis and Detection using SDS-PAGE and Western Blot (for tagged protein) Bradford assay for quantitation Protein delivery	≤1 week

^*All timelines are approximate. In most cases, we deliver within 4-6 weeks. However, optional steps might prolong the overall timeline slightly

For large molecular weight, challenging proteins, we offer high quality customized bacterial protein expression services. For any large scale protein production requirements, you could try our fully customizable E. coli fermentation services. Talk to our technical account managers for more details

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

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  Email: protein@genscript.com
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  Phone: 1-8-436-7274   (Toll-Free) 1-732-885-9188
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  Fax: 1-732-210-0262 1-732-885-5878