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After digestion of a DNA with certain Restriction enzymes, the ends left have one strand overhanging the other to form a short (typically 4 nt) single-stranded segment. This overhang will easily re-attach to other ends like it, and are thus known as "Sticky ends". For example, the enzyme BamHI recognizes the sequence GGATCC, and clips after the first G in each strand: The overhangs thus produced can still hybridize ("anneal") with each other, even if they came from different parent DNA molecules, and the enzyme ligase will then covalently link the strands. Sticky ends therefore facilitate the ligation of diverse segments of DNA, and allow the formation of novel DNA constructs.
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
Gene Synthesis
Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.
ORF cDNA Clones and Custom Clones
Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.
Plasmid DNA Preparation
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.
Express Cloning & Subcloning
$49 and 2 day turnaround, choose from over 150 IP FREE vectors.
Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Oligo Synthesis
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]
