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PCR reagents

PCR (Polymerase Chain Reaction) reagents are a set of essential chemical components and materials used in the PCR technique, a molecular biology method employed to amplify specific DNA sequences. PCR is a powerful and widely used tool for various applications, including DNA analysis, gene expression studies, genetic testing, and DNA sequencing. The reagents used in PCR include:

1. DNA Template: This is the target DNA that contains the sequence of interest, such as genomic DNA, plasmid DNA, cDNA, or RNA (which can be reverse transcribed into cDNA).

2. Primers: Short, single-stranded DNA molecules that are complementary to the sequences flanking the target region. Primers are essential for initiating DNA synthesis during PCR.

3. DNA Polymerase: An enzyme that synthesizes new DNA strands by adding complementary nucleotides to the primers. The most commonly used DNA polymerase for PCR is Taq polymerase, but there are also various other polymerases available for specific applications.

4. Nucleotides (dNTPs): Deoxyribonucleotide triphosphates, which are the building blocks for synthesizing new DNA strands. They include dATP, dCTP, dGTP, and dTTP.

5. Buffer Solution: A buffer containing salts and pH stabilizers to maintain the optimal pH for the DNA polymerase activity. It provides a stable environment for the PCR reaction.

6. Magnesium (Mg²⁺) ions: Essential for the activity of DNA polymerase, as they serve as cofactors.

7. Thermal Cycler: The PCR machine that controls the temperature cycles for denaturation, annealing, and extension during the PCR reaction.

8. Reaction Tubes/Plates: The vessels in which the PCR reaction takes place, typically made of thin-walled plastic tubes or plates.

9. PCR Machine: A specialized instrument that automates the temperature cycling necessary for PCR, maintaining the reaction at specific temperatures for denaturation, annealing, and extension.

10. PCR Reagent Kits: Commercially available kits that often contain all the necessary reagents in a convenient package, reducing the need for individual reagent procurement.

11. PCR Consumables: These include pipette tips, microcentrifuge tubes, and other disposable items used for handling samples and reagents.

PCR reagents and equipment are fundamental tools in molecular biology and genetics research, enabling the amplification and analysis of DNA sequences with high precision and reproducibility. They are used in a wide range of applications, from basic research to clinical diagnostics and forensics.

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

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