A method for determination of zygosity. Three primers are used, two of which are allele-specific and one of which matches the complementary strand of both alleles. allele specificity is achieved by designing mismatches to the unwanted allele at the 3' end of the primer. However, precisely because the primers must be chosen to generate amplicons of characteristic size, they will have different efficiencies of amplification, which will cloud the interpretation of results.(see bidirectional PCR amplification of specific alleles; PCR amplification of specific alleles) Lu, Q., Thorland, E.C., Heit, J.A. and Sommer, S.S. (1997) genome Res. 7, 389-398
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
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Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]