BLI & SPR Real-time Affinity Measurement Services

BLI vs. SPR

BLI High Throughput Package

For high-throughput demands, our proprietary BLI HT service accelerates early-stage discovery—classifying molecular binding as positive, negative, or ambiguous. We offer cost-efficient bundles that seamlessly integrate gene-to-protein expression and affinity measurement in as fast as 8 days.

Discover how our tailored bundles and exclusive promotions can accelerate your projects —get in touch to learn more!

Applications in Antibody Drug Discovery

BLI and SPR are essential technologies in protein discovery, unravelling precise biomolecular interaction insights critical for characterizing therapeutic antibodies. Explore our interactive workflow diagram to see how these platforms deliver value at every stage of antibody discovery!

Beyond antibodies? Our BLI/SPR solutions power diverse applications—Book a call to tell us your focus!

Gene to Hits

Hits to Leads

Candidates Selection

Discovery & Target
Identification

Hits Screening

Lead Indentifaction
& Validation

Lead Optimization

In vivo & vitro Assays

GLP Tox

0.05mg

500mg

1g

200g

TurboCHO™ HT

TurboCHO™ Express

TurboCHO™ HP/TurboCHO™ Stable

Target Identification

• Protein-protein interactions
• Protein-small molecule/natural
  product/extract interactions
• Protein-DNA/RNA/Peptide interactions

Lead Identification

• Compound library/clone screening
  (hybridoma, phage display, lysate,
  cell expression supernatant)
• Affinity/k_off ranking
• Epitope binning

Lead Optimization

• Fc engineering/humanization
• FcγRs/FcRn binding activity
• Epitope mapping

Case Studies

• Case 1 SPR – IgG Affinity –SPR has high sensitivity and broad affinity detection range (KD = 10^-3-10^-15)

  As shown in Fig 1 and Tab 1, SPR has higher sensitivity and a wider affinity detection range than BLI. SPR is suitable for both low-affinity (KD ~µM) and high-affinity (KD ~0.1 nM) samples, which may not be successfully detected by BLI.

  Figure 1 Affinity binding curves of human IgG-1, 2, 3

  Human IgG-1

  

  Human IgG-2

  

  Human IgG-3

  

  Table 1 Affinity binding detailed analysis report of human IgG-1, 2, 3

  Ab	Chip	Affinity	Test item	Chi² (RU²)	Ka (1/Ms)	kdis (1/s)	KD (M)
  Human IgG-1	ProA	Medium	Affinity	4.90e-02	1.39e+05	2.07e-03	1.49e-08
  Human IgG-2	ProA	High	Affinity	3.41e-01	2.75e+04	3.59e-05	1.30e-09
  Human IgG-3	ProA	Low	Kinetics	4.75	-	-	3.818E-07

• Case2 BLI v.s. SPR – IgG Affinity - BLI and SPR results are comparable for most antibody-antigen interactions

  Most antigen-antibody interactions fall into the category of medium affinity samples, for which the detection results (KD) of BLI and SPR are nearly identical.

  Rabbit IgG

  

  Rabbit IgG

  

  Mouse IgG

  
• Case3 BLI – Expression Supernatant – BLI is suitable for the affinity measurement of crude samples

  The absence of a flow channel system in BLI simplifies system maintenance and enhances compatibility with crude samples, such as unpurified expression supernatants.