ankle1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankle1
Entrez Gene ID	100498215
Full Name	ankyrin repeat and LEM domain containing 1
Synonyms	ankrd41,lem3,lemd6
General protein information	Preferred Names ankyrin repeat and LEM domain containing 1 Names ankyrin repeat and LEM domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937942.5	XP_002937988.3	ankyrin repeat and LEM domain-containing protein 1
XM_002937942.4	XP_002937988.2	ankyrin repeat and LEM domain-containing protein 1 isoform X1
XM_018097004.1	XP_017952493.1	ankyrin repeat and LEM domain-containing protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following ankle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa48525	XM_002937942.5 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa04288	XM_002937942.4 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OXa42033	XM_018097004.1 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankle1 ( XM_002937942.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937988.3 Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.

Price & Availability↑

CloneID	OXa48525
Clone ID Related Accession (Same CDS sequence)	XM_002937942.5
Accession Version	XM_002937942.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937942.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTATTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAA TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAAAT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG 
AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA 
TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG 
CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT 
CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG 
GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG 
CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG 
CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937988.3
CDS	255..4397
Translation	MDTLALARLLCEAVENEDAKEVENLLKNGADPNLVLPTGIAAIHLAAGKENESALRCLTLILQEGGDPNVRSMELLTPVHVAASWGCHKALVFLLRKGGNPTLEDQEGNTASDLALMEGNRRCVVALQEYDRSVEIPWMEENRGYGNYDDITQISSISLLLESTDDNSPYSSTKIYPFSPMCKKENITQGMNNAVVTSQLDPPGRDGKINSQGERSVDLKIKTHQKNEGSSEHLSVSLQTIHNDFCEDNLTDFGVLCSTKLSQTSHCIADASDNLYIDLIDTGVLPQEEQKENNNLSNNSQTLMCNASNLSQNVNITQNQMEKDICSNSTNTSAKRFLKSIGELILDFSTSSRLIDSNSRSRQIKGLDVTSPDHVYIFSKSKTTYDDSLEKTFILDSSNGVNAEEKDQSSSSNYNSCESECFASLGESPSSSEKKKRSKRDSGCCISPKNSTNVYNLKNGEEKADIKMSVFETDDTDSLGQHQNPVHNSQCPGERDSQLPCKSQSATLCVGYAGEHTNLNVKSSKGQSRNHMFCNKDFLANNKSQICLHDSNALKDNNQIKLTREEQHHVLGRQHNDIIVLTVQQDGTSNLMAKPDILTIEGAAHDLHDTSNVNVDHVFNNQLNDMMILTKQQISPSNFTSTCGTQIQIYDSDTLPIASQIHLNGKENKVLDGQLKDMLFLTKQQVISSNFIATSDTLSVEPGTEDVHDNADPEQEIIKQLKDMMFLTKQQVSHCNFTPIPETQTEGYDSDTLPVTNQTSLTGEKAKVLDGQLKHMMFLTKQQVSPSNFLATSDTFVVESTAEDVQDNSNADLNTELRKIMMLTKTSQSHPDQEKSFCHFTPRTKSRLLSSSSRSSSSLFDETVEMPQRGRRIRSPDAQKSPYGSAMSGSRRGLFHCSLACDQAENSASCADRQKIQQAAAVRNVNISNFLTDDLTSSDTEGSKSDTRCHQKCESHGADSVVSATTWLTEDGEDESCGDTVHVDNMQQSTRLNDLVAKDIPTRKVSRYSFSRLSCVLKSDDGAPNSCQLNTVNESSVQDVPLSPGGRPLNESTAEPVEYLYMDCERGHSLIERHVPCTDESATEISENSDDTIIYDWREYKNNKQNVIQGQIAPSNKVSVELYRLSNNDIASRLNELGEETGPVTSQTRKMYIALLDKRLKESSVRGSTGISGFSRELSLALRSFQIPDSSTDESTLSKEFDKPDKSQKWREGVLKSSFNYLLLDPRVTRNLPARCRTLSKPDCFRTFISSIFYVGKGKRSRPYFHLYEALTHYKSKNKKPCPKVQHILDIWNSGHGVISLHCFQNTIPVEAYTREACMVDTIGLKMLTNQKKGVYYGQLQSWSPSRRQLLGVHMLHRAMQIFLAEGERQLRPPDIRSGS

Target ORF information:

RefSeq Version	XM_002937942.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937942.5

ORF Insert Sequence:

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTATTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAA TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAAAT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG 
AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA 
TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG 
CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT 
CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG 
GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG 
CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG 
CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle1 ( XM_002937942.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937988.2 Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04288
Clone ID Related Accession (Same CDS sequence)	XM_002937942.4
Accession Version	XM_002937942.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937942.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG 
AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA 
TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG 
CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT 
CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG 
GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG 
CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG 
CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937988.2
CDS	252..4394
Translation	MDTLALARLLCEAVENEDAKEVENLLKNGADPNLVLPTGIAAIHLAAGKENESALRCLTLILQEGGDPNVRSMELLTPVHVAASWGCHKALVFLLRKGGNPTLEDQEGNTASDLALMEGNRRCVVALQEYDRSVEIPWMEENRGYGNYDDITQISSISLLLESTDDNSPYSSTKIYPFSPMCKKENITQGMNNAVVTSQLDPPGRDGKINSQGERSVDLKIKTHQKNEGSSEHLSVSLQTIHNDFCEDNLTDFGVLCSTKLSQTSHCIADASDNLYIDLIDTGVLPQEEQKENNNLSNNSQTLMCNASNLSQNVNITQNQMEKDICSNSTNTSAKRFLKSIGELILDFSTSSRLIDSNSRSRQIKGLDVTSPDHVYIFSKSKTTYDDSLEKTFILDSSNGVNAEEKDQSSSSNYNSCESECFASLGESPSSSEKKKRSKRDSGCCISPKNSTNVYNLKNGEEKADIKMSVFETDDTDSLGQHQNPVHNSQCPGERDSQLPCKSQSATLCVGYAGEHTNLNVKSSKGQSRNHMFCNKDFLANNKSQICLHDSNALKDNNQIKLTREEQHHVLGRQHNDIIVLTVQQDGTSNLMAKPDILTIEGAAHDLHDTSNVNVDHVFNNQLNDMMILTKQQISPSNFTSTCGTQIQIYDSDTLPIASQIHLNGKENKVLDGQLKDMLFLTKQQVISSNFIATSDTLSVEPGTEDVHDNADPEQEFIKQLKDMMFLTKQQVSHCNFTPIPETQTEGYDSDTLPVTNQTSLTGEKAKVLDGQLKHMMFLTKQQVSPSNFLATSDTFVVESTAEDVQDNSNADLNTELRKIMMLTKTSQSHPDQEKSFCHFTPRTKSRLLSSSSRSSSSLFDETVEMPQRGRRIRSPDAQKSPYGSAMSGSRRGLFHCSLACDQAENSASCADRQKIQQAAAVRNVNISNFLTDDLTSSDTEGSKSDTRCHQKCESHGADSVVSATTWLTEDGEDESCGDTVHVDNMQQSTRLNDLVAKDIPTRKVSRYSFSRLSCVLKSDDGAPNSCQLNTVNESSVQDVPLSPGGRPLNESTAEPVEYLYMDCERGHSLIERHVPCTDESATEISENSDDTIIYDWREYKNNKQNVIQGQIAPSNKVSVELYRLSNNDIASRLNELGEETGPVTSQTRKMYIALLDKRLKESSVRGSTGISGFSRELSLALRSFQIPDSSTDESTLSKEFDKPDKSQKWREGVLKSSFNYLLLDPRVTRNLPARCRTLSKPDCFRTFISSIFYVGKGKRSRPYFHLYEALTHYKSKNKKPCPKVQHILDIWNSGHGVISLHCFQNTIPVEAYTREACMVDTIGLKMLTNQKKGVYYGQLQSWSPSRRQLLGVHMLHRAMQIFLAEGERQLRPPDIRSGS

Target ORF information:

RefSeq Version	XM_002937942.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937942.4

ORF Insert Sequence:

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG AGTCACTCGG AATCTGCCTG CACGCTGTCG TACTCTTTCG 
AAGCCTGACT GCTTTCGGAC ATTCATCAGC TCTATCTTTT ATGTGGGCAA AGGAAAAAGA 
TCGCGTCCAT ACTTCCACCT CTATGAGGCC TTAACCCACT ACAAAAGCAA GAACAAAAAG 
CCTTGTCCCA AGGTGCAGCA CATTCTGGAC ATTTGGAATA GTGGCCATGG GGTGATTTCT 
CTACACTGTT TCCAGAACAC CATTCCCGTG GAAGCGTATA CACGAGAAGC ATGCATGGTG 
GATACAATTG GTTTGAAGAT GTTAACCAAT CAAAAGAAGG GTGTTTACTA TGGCCAGTTG 
CAGAGTTGGA GCCCCAGTCG CCGTCAGCTG CTTGGTGTAC ACATGTTGCA CAGAGCAATG 
CAGATCTTCT TGGCTGAGGG AGAACGTCAG CTTCGACCCC CAGACATACG CTCAGGTTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle1 ( XM_018097004.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952493.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X2, mRNA.

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CloneID	OXa42033
Clone ID Related Accession (Same CDS sequence)	XM_018097004.1
Accession Version	XM_018097004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG TTTCGTCCAC AGATTCCCAC CTCGTTCATC TGGAAACACC 
CTGTCAATGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952493.1
CDS	255..3989
Translation	MDTLALARLLCEAVENEDAKEVENLLKNGADPNLVLPTGIAAIHLAAGKENESALRCLTLILQEGGDPNVRSMELLTPVHVAASWGCHKALVFLLRKGGNPTLEDQEGNTASDLALMEGNRRCVVALQEYDRSVEIPWMEENRGYGNYDDITQISSISLLLESTDDNSPYSSTKIYPFSPMCKKENITQGMNNAVVTSQLDPPGRDGKINSQGERSVDLKIKTHQKNEGSSEHLSVSLQTIHNDFCEDNLTDFGVLCSTKLSQTSHCIADASDNLYIDLIDTGVLPQEEQKENNNLSNNSQTLMCNASNLSQNVNITQNQMEKDICSNSTNTSAKRFLKSIGELILDFSTSSRLIDSNSRSRQIKGLDVTSPDHVYIFSKSKTTYDDSLEKTFILDSSNGVNAEEKDQSSSSNYNSCESECFASLGESPSSSEKKKRSKRDSGCCISPKNSTNVYNLKNGEEKADIKMSVFETDDTDSLGQHQNPVHNSQCPGERDSQLPCKSQSATLCVGYAGEHTNLNVKSSKGQSRNHMFCNKDFLANNKSQICLHDSNALKDNNQIKLTREEQHHVLGRQHNDIIVLTVQQDGTSNLMAKPDILTIEGAAHDLHDTSNVNVDHVFNNQLNDMMILTKQQISPSNFTSTCGTQIQIYDSDTLPIASQIHLNGKENKVLDGQLKDMLFLTKQQVISSNFIATSDTLSVEPGTEDVHDNADPEQEFIKQLKDMMFLTKQQVSHCNFTPIPETQTEGYDSDTLPVTNQTSLTGEKAKVLDGQLKHMMFLTKQQVSPSNFLATSDTFVVESTAEDVQDNSNADLNTELRKIMMLTKTSQSHPDQEKSFCHFTPRTKSRLLSSSSRSSSSLFDETVEMPQRGRRIRSPDAQKSPYGSAMSGSRRGLFHCSLACDQAENSASCADRQKIQQAAAVRNVNISNFLTDDLTSSDTEGSKSDTRCHQKCESHGADSVVSATTWLTEDGEDESCGDTVHVDNMQQSTRLNDLVAKDIPTRKVSRYSFSRLSCVLKSDDGAPNSCQLNTVNESSVQDVPLSPGGRPLNESTAEPVEYLYMDCERGHSLIERHVPCTDESATEISENSDDTIIYDWREYKNNKQNVIQGQIAPSNKVSVELYRLSNNDIASRLNELGEETGPVTSQTRKMYIALLDKRLKESSVRGSTGISGFSRELSLALRSFQIPDSSTDESTLSKEFDKPDKSQKWREGVLKSSFNYLLLDPSFVHRFPPRSSGNTLSMT

Target ORF information:

RefSeq Version	XM_018097004.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 1 (ankle1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097004.1

ORF Insert Sequence:

ATGGACACGC TGGCTTTGGC TCGGCTTTTG TGCGAAGCTG TGGAGAATGA AGACGCAAAG 
GAGGTTGAAA ATCTATTAAA AAATGGGGCA GACCCAAACC TTGTTTTGCC CACTGGTATT 
GCAGCCATCC ACCTGGCTGC TGGGAAAGAG AATGAGAGTG CCCTAAGGTG TCTAACGCTA 
ATCCTGCAGG AAGGAGGGGA TCCCAATGTG AGATCCATGG AACTGTTGAC TCCTGTACAT 
GTTGCTGCAT CCTGGGGCTG TCATAAGGCA CTGGTGTTCT TGTTGAGGAA AGGTGGTAAC 
CCCACTCTTG AAGATCAGGA AGGGAATACT GCATCAGATT TGGCGTTAAT GGAGGGGAAT 
CGGAGATGTG TTGTGGCTCT GCAGGAGTAT GACAGGAGTG TGGAAATCCC ATGGATGGAA 
GAAAACCGCG GTTATGGAAA CTATGATGAT ATAACACAAA TTAGCAGCAT TTCTCTCCTT 
CTTGAGTCTA CAGATGACAA CTCTCCATAC AGTAGCACCA AAATATATCC TTTTTCTCCT 
ATGTGCAAGA AAGAAAATAT AACACAGGGC ATGAATAATG CAGTGGTGAC AAGCCAACTG 
GATCCTCCAG GCAGGGATGG TAAAATCAAC TCACAGGGGG AACGAAGTGT TGACTTGAAG 
ATCAAGACAC ACCAGAAAAA TGAGGGATCA TCAGAACACC TAAGTGTTAG CTTGCAAACC 
ATCCACAATG ATTTCTGTGA AGATAATCTA ACTGACTTTG GAGTACTGTG CAGCACCAAG 
CTTTCACAAA CCAGCCACTG CATTGCAGAT GCCTCTGATA ATCTATATAT TGATCTCATA 
GATACAGGTG TTTTACCCCA AGAAGAACAA AAAGAAAACA ATAACCTGTC AAATAATTCT 
CAAACCTTGA TGTGCAATGC GAGTAATCTG TCACAAAATG TTAATATCAC CCAAAATCAA 
ATGGAAAAGG ACATTTGTAG CAATAGTACT AATACATCTG CAAAAAGATT TTTAAAATCT 
ATTGGAGAGC TTATTCTGGA CTTTTCCACC AGCAGTCGAC TAATAGACTC CAATTCCCGT 
TCAAGACAAA TAAAAGGTTT AGATGTAACG TCACCAGACC ATGTCTATAT CTTCAGCAAA 
AGCAAAACCA CCTATGATGA TAGCTTGGAG AAGACTTTTA TTCTTGATAG CAGTAATGGT 
GTTAATGCAG AAGAAAAGGA TCAAAGTTCC AGCAGCAATT ACAACAGTTG TGAGAGTGAG 
TGTTTTGCTA GTCTTGGAGA AAGTCCTTCC TCATCTGAAA AGAAAAAGAG GTCAAAACGG 
GACTCTGGAT GCTGCATCAG TCCAAAAAAT AGCACAAATG TTTACAATCT AAAAAATGGT 
GAGGAAAAGG CAGATATTAA GATGTCAGTA TTTGAAACTG ATGATACTGA TTCTCTAGGG 
CAACATCAGA ATCCTGTGCA CAACAGTCAG TGTCCTGGAG AGAGAGACTC ACAACTTCCA 
TGCAAATCTC AGTCTGCAAC ACTGTGTGTA GGTTATGCTG GTGAACATAC TAATTTAAAT 
GTTAAAAGTT CAAAAGGCCA GTCAAGGAAT CATATGTTTT GCAACAAGGA CTTTCTTGCA 
AACAACAAAA GCCAAATCTG TTTGCATGAT TCAAACGCGC TTAAAGATAA TAATCAGATA 
AAACTGACTA GGGAAGAACA ACACCATGTA CTGGGTAGGC AACATAACGA TATCATAGTT 
TTGACCGTGC AACAAGACGG AACATCTAAT TTGATGGCTA AACCAGACAT ACTCACAATA 
GAAGGAGCTG CACATGATTT GCATGATACA AGTAATGTTA ATGTAGACCA TGTATTTAAT 
AACCAGTTAA ACGATATGAT GATTTTGACC AAGCAGCAAA TCAGTCCCAG TAATTTTACA 
TCCACATGTG GAACCCAAAT CCAGATTTAT GATTCAGACA CTCTTCCAAT TGCTAGTCAG 
ATACATCTGA ATGGGAAAGA AAACAAGGTA CTGGATGGGC AATTAAAAGA TATGTTGTTT 
TTGACGAAGC AACAAGTTAT TTCCTCTAAT TTCATTGCTA CATCTGACAC ACTTTCAGTA 
GAACCAGGTA CAGAAGATGT GCATGATAAT GCTGATCCAG AACAGGAATT TATTAAGCAG 
CTGAAAGATA TGATGTTTTT AACCAAGCAA CAAGTCAGTC ACTGTAATTT TACACCCATC 
CCTGAAACAC AAACTGAGGG ATATGATTCG GACACTCTTC CAGTTACCAA TCAGACAAGT 
CTAACTGGGG AAAAAGCCAA GGTACTGGAT GGGCAATTGA AACATATGAT GTTTTTGACC 
AAGCAACAAG TCAGCCCCTC TAATTTCTTG GCTACATCTG ACACATTTGT AGTGGAGTCA 
ACTGCAGAAG ATGTGCAAGA TAATAGTAAT GCCGATCTAA ACACAGAGCT GAGGAAAATA 
ATGATGTTAA CTAAGACTTC CCAGTCTCAT CCAGATCAGG AAAAAAGCTT TTGCCATTTC 
ACTCCTCGTA CAAAAAGCCG TCTTCTTTCT TCCAGTTCTC GCTCTAGCAG TTCACTTTTT 
GATGAGACAG TGGAAATGCC CCAGCGAGGA AGAAGAATCC GAAGCCCAGA TGCTCAAAAG 
TCTCCGTATG GGTCAGCCAT GAGTGGCTCC AGGAGAGGTT TGTTTCATTG TAGCTTGGCA 
TGTGACCAAG CAGAAAATTC AGCTTCATGT GCTGATAGAC AGAAAATACA ACAGGCAGCT 
GCTGTAAGAA ATGTAAATAT TAGCAACTTT CTAACTGATG ACCTGACCTC TTCAGATACA 
GAAGGATCAA AGTCAGATAC ACGTTGCCAC CAAAAATGTG AAAGCCATGG AGCAGATAGT 
GTGGTGTCTG CAACCACATG GCTTACAGAG GATGGAGAAG ATGAAAGTTG TGGTGATACT 
GTCCATGTAG ATAATATGCA GCAGAGCACT AGGTTGAATG ACTTAGTAGC AAAAGATATA 
CCTACAAGAA AGGTGTCACG TTACAGCTTC AGTAGACTAT CTTGTGTTCT GAAATCAGAT 
GATGGGGCAC CAAACTCTTG CCAGCTTAAT ACAGTTAATG AGTCTTCTGT CCAAGATGTA 
CCTCTATCTC CTGGTGGCCG GCCTTTGAAT GAGAGTACGG CTGAACCTGT GGAATATCTT 
TATATGGATT GTGAAAGGGG CCATTCTCTT ATAGAAAGAC ATGTTCCTTG TACAGATGAG 
TCTGCTACTG AAATCAGTGA GAATTCTGAT GATACAATTA TTTATGACTG GAGGGAGTAT 
AAGAACAACA AGCAAAATGT TATCCAGGGA CAAATAGCAC CTTCTAATAA AGTGTCTGTT 
GAATTGTACA GACTGTCTAA TAATGATATT GCAAGCAGGC TAAATGAGCT GGGAGAGGAA 
ACAGGACCTG TAACCAGCCA AACCAGAAAA ATGTACATTG CTCTACTAGA TAAGCGTCTG 
AAAGAATCAT CGGTTAGAGG ATCCACTGGA ATTTCAGGAT TCAGCCGAGA ACTTTCCCTA 
GCTCTCCGCA GTTTTCAGAT ACCTGACAGT AGCACAGATG AATCTACACT ATCTAAAGAA 
TTTGACAAGC CGGATAAGAG CCAAAAGTGG AGAGAGGGGG TTCTAAAATC CAGTTTTAAT 
TACTTGCTGC TAGACCCCAG TTTCGTCCAC AGATTCCCAC CTCGTTCATC TGGAAACACC 
CTGTCAATGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ankle1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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