NUP214 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP214
Entrez Gene ID	427760
Full Name	nucleoporin 214
General protein information	Preferred Names nucleoporin 214 Names nuclear pore complex protein Nup214 nucleoporin 214kDa
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015279736.1	XP_015135222.1	nuclear pore complex protein Nup214 isoform X1
XM_015279738.1	XP_015135224.1	nuclear pore complex protein Nup214 isoform X3
XM_015279737.1	XP_015135223.1	nuclear pore complex protein Nup214 isoform X2
XM_015279738.2	XP_015135224.2	LOW QUALITY PROTEIN: nuclear pore complex protein Nup214

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NUP214	XP_001167010.3
	NUP214	XP_001118571.2
Gallus gallus (chicken)	NUP214	XP_425333.4
Homo sapiens (human)	NUP214	NP_005076.3
Mus musculus (house mouse)	Nup214	NP_758472.2
Rattus norvegicus (Norway rat)	Nup214	NP_001162031.1
Canis lupus familiaris (dog)	NUP214	XP_850680.3
Bos taurus (cattle)	NUP214	NP_001192372.1

Gene Ontology

The following NUP214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145328	XM_015279738.2 Latest version!	Gallus gallus nucleoporin 214 (NUP214), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa43655	XM_015279736.1 Latest version!	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43656	XM_015279737.1 Latest version!	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa43657	XM_015279738.1 Latest version!	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP214 ( XM_015279738.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135224.2 Gallus gallus nucleoporin 214 (NUP214), mRNA.

Price & Availability↑

CloneID	OGa145328
Clone ID Related Accession (Same CDS sequence)	XM_015279738.2
Accession Version	XM_015279738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	LOW QUALITY PROTEIN: nuclear pore complex protein Nup214
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006104.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015279738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCC 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC CCCAAAATCA CCGGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGCATCCATA 
ACACCAGCTC CTTCTGTGGG CACTACTGCA GCCACTGCCA CATCCTTCTC CTTTGGATCC 
ATTAACTTGA AACCATCTGC AGAAAGTTCA CCTGGGCTGC CACACTCCAC AGCTCCTGCT 
GCACAGAAGT CATCTTTTGC CCCTTCGGCT TCTTCAGTTA AAGTGAATCT CAATGAGAAG 
TTCACTGCTG TAGATACTTC ATCTCCGACT ACCAGCAGCG GTCCTACCAG TTCCACAATG 
TTTTCATTCT CAACCACTTC AAAAACTGTT TCTGGAGGTC CTCTGAGTCA GCCTGTGACT 
CTCTCAGCCT CTCCTACCAC ACCACTGAAG ACTTCAACCC CTGCACCTGC TGCAATGGCG 
CGTCCTGCTC AAAACGTGCC TGCAGCTTCC TTACTACAGA AGACAGCCAA AACCACCCCT 
TCTGCAGCAA AACCAGGCTC TACTCAGGGC AAATCAGCAC TCTCCAGTGC TGCTTTAGAC 
AAACCTGCCC ACCAGTGGAA GGATTCAGAC CCTGTCATCG CAGGAATCAG AGAGGAGATT 
GCACATTTTC AAAAGGAAAT GGAGGAGCTG AAGGCCCGGA CTTCCAAAGC CTGTTTCCAA 
GTGGGTACTC CAGAGGAAAT GAAAACGTTG CGAACTGAGT CAGATGATCT CCACACCTTC 
CTCTTGGAAA TTAAAGAAAC AACCGAGTCG CTGCATGGAG ATATTAGTAC TCTGAAGACA 
ACCTTGCTGG AAGGATTTGC AGGGGTAGAA GAGGCTAGAG AGCAGAATGA GCGGAATCAT 
AGCTTGGGGT ATTTGCACTT ACTTTATAAG AAACCTTTGG ATCCCAAGAG TGAAGCACAA 
CTTCAGGAGA TTCGACGCCT GCACCAGTAT GTAAAGTTTG CTGTTCAGGA TGTGAATGAT 
GTTCTGGATT TGGAATGGGA TCGGCACCTG GAACAGGAGA GAAAGCAGAA GCGTCTGATT 
GTTCCTGAAC GAGAGACTTT GTTTAACACC CTTGCTAATA ACCGGGAAAT AATCAATCAG 
CAGAAGAAGA GACTAAATCA GTTGGTGAAC AGCCTACAGC AGCTCCGACT CTATAATCAA 
ACATCTCAGT GGAGTGTTCC CAGTGATACA TCCTCTCCTA GCAGTACTCA AAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAGTGCCTTG ATGAAAACTA CCTTGGAATC ACGTGCCAAA 
CCCCCACCTA AAGCACCTGC TAAGTTGTCC CCTGTGAAGC TGACACAGTT GAGGAATTTT 
CTAGCCAAGA GGAAGACTCC ACCAGTTAGG TCTATGGCTC CAGCAAGTTT GTCCCGATCT 
GCCTTCTTGT CCACAAGATA CTATGAAGAC TTGGATGAAG TCAGTTCAAC ATCCTCAGTT 
TCTCAGTCTC TGGAGAATGA GGATCTGCAG GCAGCGGACC AGGAAGAGGA GGCTGCTCCC 
ATCCAAGTCC CTCGTCATGC CCCTGTGGTC CGCACTCCGT CTATCCAGCC TGGTTTGGTG 
GCTCAGCCTC CATCCTTTGG GAAACAACAC CCCTCCTTGG GCACTCTATT GCCTACATCT 
TCGAACAGAA TCATCCCACA AGGGGCAGAC AGCACAATGC TTGCAACAAA GACTGTGAAA 
CATGGGGCCC CTGCTCCCAC CGTCACTGTT CCAGCACCCC AGGCAGCTGC AGCGGCAGCC 
CTGCGGAGGC AGATGGCCAC CCAGTCCCCA GCTGTGAGCA CCTCCTTGAC TGAATCAACT 
CTGAAGAACG TTCCCCAGGT GGTGAATGTG CGAGAACTCA AGACTGGTGC GTTGACTCCA 
GCTGTCTCTA CAGTAATAGG GTCATCAGTT CCTCACTCTG CAGCCCAGGC AGTCCACCAA 
GTTCTGGCAA GTGTTGCTAC AAATCAGGCT AAGCAGACAC CTCAAACAAG CTCACTTAAG 
GGACAGGCTC CACCTTCTTC TGTATCTGCA TCTTGTCCTG TGGCATCTAC ACCTGGTCCC 
ACATCTGGCA ACAAAATCTC AGGACAAGGG ACAGCAAAAA CCGTTACAGC TGTGACCTCT 
ACAGTGGCGT CAGTACCAGC AACATCTGCA CAGCCTAACA AGGCTTTCTC ATTTTCTTCT 
GTGGGGTCTG GGTTTAATTT TGGTGTTGTC ACGTCGGCTG CTTCATCACC GACACTGCCC 
AGCCTCGCTG CCTCACAAGC CCCTGCTAAG GAGTCCACTC AACCTGATTC ATTTTCGCTT 
GCTGGGGGTT CCAAATTTGG AGCAGTCCCT GAGAGCTCCT TTGCTTTTGG CTCCCTCAAG 
CCAGCAAGTG CTTCTGGAGC CGCAGTAGCA AATAGCTCTT CAGCAGATGG CACTCAGTCA 
AACAAACCTG CTGCAACAAT GTCTTCTGCT CCATCCACAG CCTCTGGCAA ATTAGATATC 
CCTCAGTCTA AGCCAGGGGA CACCTTGTTT CTGAGCAGTC TGTCTGGGGA AACTCTCGGG 
AGCTTCTCAG GACTGCGTGT TGGTCAAGCA GATGATAATG CCAAAACCAC CACTAAAACA 
CCATCTACCA GTGCTGCAGG AGCACAGTCA ACTAAAGCAT CTACATCACC TTCTGGGTTT 
GTCTTTGGTA GCCCCCTGAT GTTAGGAAAA GCTGGAGAAG CTACTTCAAC ATCAGTCACG 
TCAGCTGGCA CGGCACCCAC CTCCATCAGC ACCAGTGCCA CAGGCAGTCT CTTTGGCAAT 
GTTCAGATAA CCAACACAGG CTCTCCTGGA GTATTTAATC TCGCAGGCTC TAGTGGAAAC 
AAACTCAACT TTTCTTTTGG TATTCAGCAA GCAAGCAGCA TGAGCACGTC CACTTCTGCC 
CCTTCTGCTC TCCCTGCTTC AACATCTCTC TCGTTCGGGA ATCTCTTGAG TCCGTCACAA 
ACTGCTGCCC CGGCTGCAAG TTCTATTAAA GGTGGAGGAG ATGCCAAACT GCTGTCTGCT 
TCAGAAAAGA CAGCTGAAAA TGAAGCACCT GAGTCTTCAC CTCCAACAAC GCAGACTCCA 
CAGCCACAGA TACAGCTTTC AGAGCCCGTT AAAGAGCCCC CTGCATCCCA GACAGCAGCT 
GGGGACACCA GCACCGTGGG CTCCAGTACT GCATCAGTAG CACCTCCCCC TGATGCCATT 
GCTACTCCTG CTTCCACAAG TGAGCTGAAG GCACCAGTGC CTCCTGCTGC CTCAGCCTCT 
GTCCCACAGG ATCAGAGCAT CTCAGAGGCG GCCTCTGCAA CAACCATTGC CTCTCCAGCA 
GAAGCAGCGA GCACACTGAC TGCTGCCGCT GGTGTTCCTA CATGTGTTCA GAGTCCAGCT 
GTTGGTGCCT CGGTGTTTGT CGCCACCTCT GCAGGCTCCT CAGTGTTCTC TCAGCCTCCA 
AATTCCAGCT CTTCTGGCTC AGCATTTAGC CAGCCTGCAG GTGATACAAC TGCCACTCCT 
TCCACACCGT CTGTTTTTGG CCAACTGCCT GCAGGTACCA CAGCATCAGC TCCATTTGGA 
CAACCCACCA CTGCCACCGT GACCACACCG ACTGCCAGCA CAGGGTTTGG TACTNTCTGC 
TTTGGCACCA CCACGACTGG GGGCTTTGGT CAGCCAGCCT TTGGCCAAGC ACCGACCTTT 
GGGCAGCCAG CAAGCAGTTC TTCAAGTGGT TTTACCTTCA ACCAGTCTGG ATTTGGGACA 
GTGCCTGCCT TTGGCCAGCC AGCAGCTTCC ACTGCAGCCT CAAGCAGCGG CAATGTATTT 
GGAGCATCTT CTAGCACCAA CAGTGCAAGC TCCTTCTCCT TCGGACAGCC ATCTGCCAGC 
ACTGGGGGCG GGATGTTTGG TCAAAGCAAC ACTCTCGTGT TTGGGCAGAG CAGCAGCTTT 
GGGCAAGGGG GATCTGTCTT TGGTAACACC GCCGCTGTTA CTACCACTAC GTCATCTGCT 
GGGTTCAGCT TCTGCCAGGC CTCAGGTTTT GGTTCTAGCA ACACAGGTTC TGTGTTTGGG 
CAAGCAGCAA ATACTGGAGG CATTGTCTTT GGGCAGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135224.2
CDS	2207..8323
Translation	MGEDGDLVPEREMKDFQFRALKKVRIFDPPDDLPKERSSLLVVSNKYGLVFAGGGTSLQIFHTRDLLMQNQLGEDPNNIVENVQGTSVPMKFPVQHLALSCDNLTLSVCMMSSDIGSFIGFFDVRTFFNQAKQQKRMFAYHKLAKDSGNAAMVNDLKWNPASPTMVAVCLSDGSISVLQVTDSVKVYATLPPSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPNLQEKKVIPYPPFYQADNPVKVLDVLWIGTYVFAIVYAAADGTLETPPEVVVAILPKKEEKRPEKFVNFMEPCYGSCAERQHHYYLNYVEEWDLILAASAASTEVSILARQGDQNWESWILEDSSRAELPVTDKSDDTLPVGVAVDYTNNMPIPISDEKTLPPAPVLILLSTDGVLCPFYMINQNPGVRSLLVTPEPLPAAGERAPKSPGSTVTAPTTPPTSLKPDAVPAVAPAPSQPPAGDGAAAFSFTPSTAKASITPAPSVGTTAATATSFSFGSINLKPSAESSPGLPHSTAPAAQKSSFAPSASSVKVNLNEKFTAVDTSSPTTSSGPTSSTMFSFSTTSKTVSGGPLSQPVTLSASPTTPLKTSTPAPAAMARPAQNVPAASLLQKTAKTTPSAAKPGSTQGKSALSSAALDKPAHQWKDSDPVIAGIREEIAHFQKEMEELKARTSKACFQVGTPEEMKTLRTESDDLHTFLLEIKETTESLHGDISTLKTTLLEGFAGVEEAREQNERNHSLGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQERKQKRLIVPERETLFNTLANNREIINQQKKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSPSSTQSFDSDLESLRSALMKTTLESRAKPPPKAPAKLSPVKLTQLRNFLAKRKTPPVRSMAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENEDLQAADQEEEAAPIQVPRHAPVVRTPSIQPGLVAQPPSFGKQHPSLGTLLPTSSNRIIPQGADSTMLATKTVKHGAPAPTVTVPAPQAAAAAALRRQMATQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSTVIGSSVPHSAAQAVHQVLASVATNQAKQTPQTSSLKGQAPPSSVSASCPVASTPGPTSGNKISGQGTAKTVTAVTSTVASVPATSAQPNKAFSFSSVGSGFNFGVVTSAASSPTLPSLAASQAPAKESTQPDSFSLAGGSKFGAVPESSFAFGSLKPASASGAAVANSSSADGTQSNKPAATMSSAPSTASGKLDIPQSKPGDTLFLSSLSGETLGSFSGLRVGQADDNAKTTTKTPSTSAAGAQSTKASTSPSGFVFGSPLMLGKAGEATSTSVTSAGTAPTSISTSATGSLFGNVQITNTGSPGVFNLAGSSGNKLNFSFGIQQASSMSTSTSAPSALPASTSLSFGNLLSPSQTAAPAASSIKGGGDAKLLSASEKTAENEAPESSPPTTQTPQPQIQLSEPVKEPPASQTAAGDTSTVGSSTASVAPPPDAIATPASTSELKAPVPPAASASVPQDQSISEAASATTIASPAEAASTLTAAAGVPTCVQSPAVGASVFVATSAGSSVFSQPPNSSSSGSAFSQPAGDTTATPSTPSVFGQLPAGTTASAPFGQPTTATVTTPTASTGFGTnullCFGTTTTGGFGQPAFGQAPTFGQPASSSSSGFTFNQSGFGTVPAFGQPAASTAASSSGNVFGASSSTNSASSFSFGQPSASTGGGMFGQSNTLVFGQSSSFGQGGSVFGNTAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGGIVFGQQSSSSTGGLFGSGSGGRGGGFFTGLGGKPSQDAANKNPFGSSSGGFGSAASQNSSSLFGNSGAKAFGFGNSSFGDQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFSTLANQNTPTFGSLSQQGTGFGTQNSGFSAFGTVGGGFGFGSTNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_015279738.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nucleoporin 214 (NUP214), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279738.2

ORF Insert Sequence:

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCC 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC CCCAAAATCA CCGGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGCATCCATA 
ACACCAGCTC CTTCTGTGGG CACTACTGCA GCCACTGCCA CATCCTTCTC CTTTGGATCC 
ATTAACTTGA AACCATCTGC AGAAAGTTCA CCTGGGCTGC CACACTCCAC AGCTCCTGCT 
GCACAGAAGT CATCTTTTGC CCCTTCGGCT TCTTCAGTTA AAGTGAATCT CAATGAGAAG 
TTCACTGCTG TAGATACTTC ATCTCCGACT ACCAGCAGCG GTCCTACCAG TTCCACAATG 
TTTTCATTCT CAACCACTTC AAAAACTGTT TCTGGAGGTC CTCTGAGTCA GCCTGTGACT 
CTCTCAGCCT CTCCTACCAC ACCACTGAAG ACTTCAACCC CTGCACCTGC TGCAATGGCG 
CGTCCTGCTC AAAACGTGCC TGCAGCTTCC TTACTACAGA AGACAGCCAA AACCACCCCT 
TCTGCAGCAA AACCAGGCTC TACTCAGGGC AAATCAGCAC TCTCCAGTGC TGCTTTAGAC 
AAACCTGCCC ACCAGTGGAA GGATTCAGAC CCTGTCATCG CAGGAATCAG AGAGGAGATT 
GCACATTTTC AAAAGGAAAT GGAGGAGCTG AAGGCCCGGA CTTCCAAAGC CTGTTTCCAA 
GTGGGTACTC CAGAGGAAAT GAAAACGTTG CGAACTGAGT CAGATGATCT CCACACCTTC 
CTCTTGGAAA TTAAAGAAAC AACCGAGTCG CTGCATGGAG ATATTAGTAC TCTGAAGACA 
ACCTTGCTGG AAGGATTTGC AGGGGTAGAA GAGGCTAGAG AGCAGAATGA GCGGAATCAT 
AGCTTGGGGT ATTTGCACTT ACTTTATAAG AAACCTTTGG ATCCCAAGAG TGAAGCACAA 
CTTCAGGAGA TTCGACGCCT GCACCAGTAT GTAAAGTTTG CTGTTCAGGA TGTGAATGAT 
GTTCTGGATT TGGAATGGGA TCGGCACCTG GAACAGGAGA GAAAGCAGAA GCGTCTGATT 
GTTCCTGAAC GAGAGACTTT GTTTAACACC CTTGCTAATA ACCGGGAAAT AATCAATCAG 
CAGAAGAAGA GACTAAATCA GTTGGTGAAC AGCCTACAGC AGCTCCGACT CTATAATCAA 
ACATCTCAGT GGAGTGTTCC CAGTGATACA TCCTCTCCTA GCAGTACTCA AAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAGTGCCTTG ATGAAAACTA CCTTGGAATC ACGTGCCAAA 
CCCCCACCTA AAGCACCTGC TAAGTTGTCC CCTGTGAAGC TGACACAGTT GAGGAATTTT 
CTAGCCAAGA GGAAGACTCC ACCAGTTAGG TCTATGGCTC CAGCAAGTTT GTCCCGATCT 
GCCTTCTTGT CCACAAGATA CTATGAAGAC TTGGATGAAG TCAGTTCAAC ATCCTCAGTT 
TCTCAGTCTC TGGAGAATGA GGATCTGCAG GCAGCGGACC AGGAAGAGGA GGCTGCTCCC 
ATCCAAGTCC CTCGTCATGC CCCTGTGGTC CGCACTCCGT CTATCCAGCC TGGTTTGGTG 
GCTCAGCCTC CATCCTTTGG GAAACAACAC CCCTCCTTGG GCACTCTATT GCCTACATCT 
TCGAACAGAA TCATCCCACA AGGGGCAGAC AGCACAATGC TTGCAACAAA GACTGTGAAA 
CATGGGGCCC CTGCTCCCAC CGTCACTGTT CCAGCACCCC AGGCAGCTGC AGCGGCAGCC 
CTGCGGAGGC AGATGGCCAC CCAGTCCCCA GCTGTGAGCA CCTCCTTGAC TGAATCAACT 
CTGAAGAACG TTCCCCAGGT GGTGAATGTG CGAGAACTCA AGACTGGTGC GTTGACTCCA 
GCTGTCTCTA CAGTAATAGG GTCATCAGTT CCTCACTCTG CAGCCCAGGC AGTCCACCAA 
GTTCTGGCAA GTGTTGCTAC AAATCAGGCT AAGCAGACAC CTCAAACAAG CTCACTTAAG 
GGACAGGCTC CACCTTCTTC TGTATCTGCA TCTTGTCCTG TGGCATCTAC ACCTGGTCCC 
ACATCTGGCA ACAAAATCTC AGGACAAGGG ACAGCAAAAA CCGTTACAGC TGTGACCTCT 
ACAGTGGCGT CAGTACCAGC AACATCTGCA CAGCCTAACA AGGCTTTCTC ATTTTCTTCT 
GTGGGGTCTG GGTTTAATTT TGGTGTTGTC ACGTCGGCTG CTTCATCACC GACACTGCCC 
AGCCTCGCTG CCTCACAAGC CCCTGCTAAG GAGTCCACTC AACCTGATTC ATTTTCGCTT 
GCTGGGGGTT CCAAATTTGG AGCAGTCCCT GAGAGCTCCT TTGCTTTTGG CTCCCTCAAG 
CCAGCAAGTG CTTCTGGAGC CGCAGTAGCA AATAGCTCTT CAGCAGATGG CACTCAGTCA 
AACAAACCTG CTGCAACAAT GTCTTCTGCT CCATCCACAG CCTCTGGCAA ATTAGATATC 
CCTCAGTCTA AGCCAGGGGA CACCTTGTTT CTGAGCAGTC TGTCTGGGGA AACTCTCGGG 
AGCTTCTCAG GACTGCGTGT TGGTCAAGCA GATGATAATG CCAAAACCAC CACTAAAACA 
CCATCTACCA GTGCTGCAGG AGCACAGTCA ACTAAAGCAT CTACATCACC TTCTGGGTTT 
GTCTTTGGTA GCCCCCTGAT GTTAGGAAAA GCTGGAGAAG CTACTTCAAC ATCAGTCACG 
TCAGCTGGCA CGGCACCCAC CTCCATCAGC ACCAGTGCCA CAGGCAGTCT CTTTGGCAAT 
GTTCAGATAA CCAACACAGG CTCTCCTGGA GTATTTAATC TCGCAGGCTC TAGTGGAAAC 
AAACTCAACT TTTCTTTTGG TATTCAGCAA GCAAGCAGCA TGAGCACGTC CACTTCTGCC 
CCTTCTGCTC TCCCTGCTTC AACATCTCTC TCGTTCGGGA ATCTCTTGAG TCCGTCACAA 
ACTGCTGCCC CGGCTGCAAG TTCTATTAAA GGTGGAGGAG ATGCCAAACT GCTGTCTGCT 
TCAGAAAAGA CAGCTGAAAA TGAAGCACCT GAGTCTTCAC CTCCAACAAC GCAGACTCCA 
CAGCCACAGA TACAGCTTTC AGAGCCCGTT AAAGAGCCCC CTGCATCCCA GACAGCAGCT 
GGGGACACCA GCACCGTGGG CTCCAGTACT GCATCAGTAG CACCTCCCCC TGATGCCATT 
GCTACTCCTG CTTCCACAAG TGAGCTGAAG GCACCAGTGC CTCCTGCTGC CTCAGCCTCT 
GTCCCACAGG ATCAGAGCAT CTCAGAGGCG GCCTCTGCAA CAACCATTGC CTCTCCAGCA 
GAAGCAGCGA GCACACTGAC TGCTGCCGCT GGTGTTCCTA CATGTGTTCA GAGTCCAGCT 
GTTGGTGCCT CGGTGTTTGT CGCCACCTCT GCAGGCTCCT CAGTGTTCTC TCAGCCTCCA 
AATTCCAGCT CTTCTGGCTC AGCATTTAGC CAGCCTGCAG GTGATACAAC TGCCACTCCT 
TCCACACCGT CTGTTTTTGG CCAACTGCCT GCAGGTACCA CAGCATCAGC TCCATTTGGA 
CAACCCACCA CTGCCACCGT GACCACACCG ACTGCCAGCA CAGGGTTTGG TACTNTCTGC 
TTTGGCACCA CCACGACTGG GGGCTTTGGT CAGCCAGCCT TTGGCCAAGC ACCGACCTTT 
GGGCAGCCAG CAAGCAGTTC TTCAAGTGGT TTTACCTTCA ACCAGTCTGG ATTTGGGACA 
GTGCCTGCCT TTGGCCAGCC AGCAGCTTCC ACTGCAGCCT CAAGCAGCGG CAATGTATTT 
GGAGCATCTT CTAGCACCAA CAGTGCAAGC TCCTTCTCCT TCGGACAGCC ATCTGCCAGC 
ACTGGGGGCG GGATGTTTGG TCAAAGCAAC ACTCTCGTGT TTGGGCAGAG CAGCAGCTTT 
GGGCAAGGGG GATCTGTCTT TGGTAACACC GCCGCTGTTA CTACCACTAC GTCATCTGCT 
GGGTTCAGCT TCTGCCAGGC CTCAGGTTTT GGTTCTAGCA ACACAGGTTC TGTGTTTGGG 
CAAGCAGCAA ATACTGGAGG CATTGTCTTT GGGCAGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_015279736.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135222.1 Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

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CloneID	OGa43655
Clone ID Related Accession (Same CDS sequence)	XM_015279736.1
Accession Version	XM_015279736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGTGCCTAGC 
TCTACAGGCA GTGAACTTCC ACCCTACCCC TTTGCCACCG ATACCTCCAA GCAAGTCCTG 
GCTCCAAGTC TTACAGGAGC GGCAACATTC TCTTTCTCCT CTGCTTCTCC TAAGGCATCC 
ATAACACCAG CTCCTTCTGT GGGCACTACT GCAGCCACTG CCACATCCTT CTCCTTTGGA 
TCCATTAACT TGAAACCATC TGCAGAAAGT TCACCTGGGC TGCCACACTC CACAGCTCCT 
GCTGCACAGA AGTCATCTTT TGCCCCTTCG GCTTCTTCAG TTAAAGTGAA TCTCAATGAG 
AAGTTCACTG CTGTAGATAC TTCATCTCCG ACTACCAGCA GCGGTCCTAC CAGTTCCACA 
ATGTTTTCAT TCTCAACCAC TTCAAAAACT GTTTCTGGAG GTCCTCTGAG TCAGCCTGTG 
ACTCTCTCAG CCTCTCCTAC CACACCACTG AAGACTTCAA CCCCTGCACC TGCTGCAATG 
GCGCGTCCTG CTCAAAACGT GCCTGCAGCT TCCTTACTAC AGAAGACAGC CAAAACCACC 
CCTTCTGCAG CAAAACCAGG CTCTACTCAG GGCAAATCAG CACTCTCCAG CGCTGCTTTA 
GACAAACCTG CCCACCAGTG GAAGGATTCA GACCCTGTCA TCGCAGGAAT CAGAGAGGAG 
ATTGCACATT TTCAAAAGGA AATGGAGGAG CTGAAGGCCC GGACTTCCAA AGCCTGTTTC 
CAAGTGGGTA CTCCAGAGGA AATGAAAACG TTGCGAACTG AGTCAGATGA TCTCCACACC 
TTCCTCTTGG AAATTAAAGA AACAACCGAG TCGCTGCATG GAGATATTAG TACTCTGAAG 
ACAACCTTGC TGGAAGGATT TGCAGGGGTA GAAGAGGCTA GAGAGCAGAA TGAGAGGAAT 
CATAGCTTGG GGTATTTGCA CTTACTTTAT AAGAAACCTT TGGATCCCAA GAGTGAAGCA 
CAACTTCAGG AGATTCGACG CCTGCACCAG TATGTAAAGT TTGCTGTTCA GGATGTGAAT 
GATGTTCTGG ATTTGGAATG GGATCGGCAC CTGGAACAGG AGAGAAAGCA GAAGCGTCTG 
ATTGTTCCTG AACGAGAGAC TTTGTTTAAC ACCCTTGCTA ATAACCGGGA AATAATCAAT 
CAGCAGAAGA AGAGACTAAA TCAGTTGGTG AACAGCCTAC AGCAGCTCCG ACTCTATAAT 
CAAACATCTC AGTGGAGTGT TCCCAGTGAT ACATCCTCTC CTAGCAGTAC TCAAAGTTTT 
GACAGTGACC TTGAAAGCCT GCGCAGTGCC TTGATGAAAA CTACCTTGGA ATCACGTGCC 
AAACCCCCAC CTAAAGCACC TGCTAAGTTG TCCCCTGTGA AGCTGACACA GTTGAGGAAT 
TTTCTAGCCA AGAGGAAGAC TCCACCAGTT AGGTCTATGG CTCCAGCAAG TTTGTCCCGA 
TCTGCCTTCT TGTCCACAAG ATACTATGAA GACTTGGATG AAGTCAGTTC AACATCCTCA 
GTTTCTCAGT CTCTGGAGAA TGAGGATCTG CAGGCAGCGG ACCAGGAAGA GGAGGCTGCT 
CCCATCCAAG TCCCTCGTCA TGCCCCTGTG GTCCGCACTC CGTCTATCCA GCCTGGTTTG 
GTGGCTCAGC CTCCATCCTT TGGGAAACAA CACCCCTCCT TGGGCACTCT ATTGCCTACA 
TCTTCGAACA GAATCATCCC ACAAGGGGCA GACAGCACAA TGCTTGCAAC AAAGACTGTG 
AAACATGGGG CCCCTGCTCC CACCGTCACT GTTCCAGCAC CCCAGGCAGC TGCTGCGGCA 
GCCCTGCGGA GGCAGATGGC CACCCAGTCC CCAGCTGTGA GCACCTCCTT GACTGAATCA 
ACTCTGAAGA ACGTTCCCCA GGTGGTGAAT GTGCGAGAAC TCAAGACTGG TGCGTTGACT 
CCAGCTGTCT CTACAGTAAT AGGGTCATCA GTTCCTCACT CTGCAGCCCA GGCAGTCCAC 
CAAGTTCTGG CAAGTGTTGC TACAAATCAG GCTAAGCAGA CACCTCAAAC AAGCTCACTT 
AAGGGACAGG CTCCACCTTC TTCTGTATCT GCATCTTGTC CTGTGGCATC TACACCTGGT 
CCCACATCTG GCAACAAAAT CTCAGGACAA GGGACAGCAA AAACTGTTAC AGCTGTGACC 
TCTACAGTGG CGTCAGTACC AGCAACATCT GCACAGCCTA ACAAGGCTTT CTCATTTTCT 
TCTGTGGGGT CTGGGTTTAA TTTTGGTGTT GTCACGTCGG CTGCTTCATC ACCGACACTG 
CCCAGCCTTG CTGCCTCACA AGCCCCTGCT AAGGAGTCCA CTCAACCTGA TTCATTTTCG 
CTTGCTGGGG GTTCCAAATT TGGAGCAGTC CCTGAGAGCT CCTTTGCTTT TGGCTCCCTC 
AAGCCAGCAA GTGCTTCTGG AGCCGCAGTA GCAAATAGCT CTTCAGCAGA TGGCACTCAG 
TCAAACAAAC CTGCTGCAAC AATGTCTTCT GCTCCATCCA CAGCCTCTGG CAAATTAGAT 
ATCCCTCAGT CTAAGCCAGG GGACACCTTG TTTCTGAGCA GTCTGTCCGG GGAAACTCTC 
GGGAGCTTCT CAGGACTGCG TGTTGGTCAA GCAGATGATA ATGCCAAAAC CACCACTAAA 
ACACCATCTA CCAGTGCTGC AGGAGCACAG TCAACTAAAG CATCTACATC ACCTTCTGGG 
TTTGTCTTTG GTAGCCCCCT GATGTTAGGA AAAGCTGGAG AAGCTACTTC AACATCAGTC 
ACGTCAGCTG GCACGGCACC CACCTCCATC AGCACCAGTG CCACAGGCAG TCTCTTTGGC 
AATGTTCAGA TAACCAACAC AGGCTCTCCT GGAGTATTTA ATCTCGCAGG CTCTAGTGGA 
AACAAACTCA ACTTTTCTTT TGGTATTCAG CAAGCAAGCA GCATGAGCAC GTCCACTTCT 
GCCCCTTCTG CTCTCCCTGC TTCAACATCT CTCTCGTTCG GGAATCTCTT GAGTCCGTCA 
CAAACTGCTG CCCCGGCTGC AAGTTCTATT AAAGGTGGAG GAGATGCCAA ACTGCTGTCT 
GCTTCAGAAA AGACAGCTGA AAATGAAGCA CCTGAGTCTT CACCTCCAAC AACGCAGACT 
CCACAGCCAC AGATACAGCT TTCAGAGCCC GTTAAAGAGC CCCCTGCATC CCAGACAGCA 
GCTGGGGACA CCAGCACCGT GGGCTCCAGT ACTGCATCAG TAGCACCTCC CCCTGATGCC 
ATTGCTACTC CTGCTTCCAC AAGTGAGCTG AAGGCACCAG TGCCTCCTGC TGCCTCAGCC 
TCTGTCCCAC AGGATCAGAG CATCTCAGAG GCGGCCTCTG CAACAACCAT TGCCTCTCCA 
GCAGAAGCAG CGAGCACACT GACTGCTGCC GCTGGTGTTC CTACATGTGT TCAGAGTCCA 
GCTGTTGGTG CCTCGGTGTT TGTCGCCACC TCTGCAGGCT CCTCAGTGTT CTCTCAGCCT 
CCAAATTCCA GCTCTTCTGG CTCAGCATTT AGCCAGCCTG CAGGTGATAC AACTGCCACT 
CCTTCCACAC CGTCTGTTTT TGGCCAACTG CCTGCAGGTA CCACAGCATC AGCTCCATTT 
GGACAACCCA CCACTGCCAC CGTGACCACA CCGACTGCCA GCACAGGGTT TGGTACTTCT 
GCCTTTGGCA CCACCACGAC TGGGGGCTTT GGTCAGCCAG CCTTTGGCCA AGCACCGACC 
TTTGGGCAGC CAGCAAGCAG TTCTTCAAGT GGTTTTACCT TCAACCAGTC TGGATTTGGG 
ACAGTGCCTG CCTTTGGCCA GCCAGCAGCT TCCACTGCAG CCTCAAGCAG CGGCAATGTA 
TTTGGAGCAT CTTCTAGCAC CAACAGTGCA AGCTCCTTCT CCTTCGGACA GCCATCTGCC 
AGCACTGGGG GCGGGATGTT TGGTCAAAGC AACACTCTCG TGTTTGGGCA AAGCAGCAGC 
TTTGGGCAAG GGGGATCTGT CTTTGGTAAC ACCGCCGCTG TGACTACCAC TACATCATCT 
GCTGGGTTCA GCTTCTGCCA GGCCTCAGGT TTTGGTTCTA GCAACACAGG TTCTGTGTTT 
GGGCAAGCAG CAAATACTGG AGGCATTGTC TTTGGGCAGC AGTCGTCATC TTCCACTGGA 
GGCCTCTTTG GATCTGGAAG TGGTGGGAGA GGTGGAGGCT TCTTCACTGG TTTGGGAGGA 
AAGCCAAGTC AGGATGCAGC CAATAAGAAT CCTTTTGGTT CCTCCAGTGG AGGATTTGGA 
TCGGCAGCTT CCCAGAATTC TTCTAGCTTA TTTGGGAACA GTGGAGCCAA GGCCTTTGGA 
TTTGGCAACT CATCTTTTGG AGACCAGAAA CCCACAGGCA CTTTCAGCTC TGGTGGTGGC 
AGTGTGGCAT CTCAAGGCTT TGGGTTCTCT AGTCCAACCA AAGCAGGTGG CTTCGGTGCT 
GCTCCAGTGT TTGGCAGCCC TCCCACTTTT GGGGGATCCC CTGGGTTTGG AGGGGTGCCA 
GCATTCGGTT CAGCCCCAAC CTTTTCAAGC CCTCTGGGCT CGACGGGAGG CAAAGTGTTC 
GGCGAGGGTA CAGCTGCAGC CAGCGCCGGA GGATTTGGGT TTGGTGGTAC CAGTAACAAC 
ACAGCATCAT TTAGCACGCT AGCAAATCAG AATACCCCGA CGTTTGGATC GCTGTCACAG 
CAGGGTACTG GTTTTGGCAC ACAGAACAGT GGATTCTCTG CTTTCGGGAC AGTTGGAGGA 
GGGTTTGGTT TTGGATCAAC TAACACATCT TCCTCTGGAT TTAGCGGATG GAGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135222.1
CDS	116..6355
Translation	MGEDGDLVPEREMKDFQFRALKKVRIFDPPDDLPKERSSLLVVSNKYGLVFAGGGTSLQIFHTRDLLMQNQLGEDPNNIVENVQGTSVPMKFPVQHLALSCDNLTLSVCMMSSDIGSFIGFFDVRTFFNQAKQQKRMFAYHKLAKDSGNAAMVNDLKWNPASPTMVAVCLSDGSISVLQVTDSVKVYATLPPSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPNLQEKKVIPYPPFYQADNPVKVLDVLWIGTYVFAIVYAAADGTLETPPEVVVAILPKKEEKRPEKFVNFMEPCYGSCAERQHHYYLNYVEEWDLILAASAASTEVSILARQGDQNWESWILEDSSRAELPVTDKSDDTLPVGVAVDYTNNMPIPISDEKTLPPAPVLILLSTDGVLCPFYMINQNPGVRSLLVTPEPLPAAGERAPKSPGSTVTAPTTPPTSLKPDAVPAVAPAPSQPPAGDGAAAFSFTPSTAKVPSSTGSELPPYPFATDTSKQVLAPSLTGAATFSFSSASPKASITPAPSVGTTAATATSFSFGSINLKPSAESSPGLPHSTAPAAQKSSFAPSASSVKVNLNEKFTAVDTSSPTTSSGPTSSTMFSFSTTSKTVSGGPLSQPVTLSASPTTPLKTSTPAPAAMARPAQNVPAASLLQKTAKTTPSAAKPGSTQGKSALSSAALDKPAHQWKDSDPVIAGIREEIAHFQKEMEELKARTSKACFQVGTPEEMKTLRTESDDLHTFLLEIKETTESLHGDISTLKTTLLEGFAGVEEAREQNERNHSLGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQERKQKRLIVPERETLFNTLANNREIINQQKKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSPSSTQSFDSDLESLRSALMKTTLESRAKPPPKAPAKLSPVKLTQLRNFLAKRKTPPVRSMAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENEDLQAADQEEEAAPIQVPRHAPVVRTPSIQPGLVAQPPSFGKQHPSLGTLLPTSSNRIIPQGADSTMLATKTVKHGAPAPTVTVPAPQAAAAAALRRQMATQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSTVIGSSVPHSAAQAVHQVLASVATNQAKQTPQTSSLKGQAPPSSVSASCPVASTPGPTSGNKISGQGTAKTVTAVTSTVASVPATSAQPNKAFSFSSVGSGFNFGVVTSAASSPTLPSLAASQAPAKESTQPDSFSLAGGSKFGAVPESSFAFGSLKPASASGAAVANSSSADGTQSNKPAATMSSAPSTASGKLDIPQSKPGDTLFLSSLSGETLGSFSGLRVGQADDNAKTTTKTPSTSAAGAQSTKASTSPSGFVFGSPLMLGKAGEATSTSVTSAGTAPTSISTSATGSLFGNVQITNTGSPGVFNLAGSSGNKLNFSFGIQQASSMSTSTSAPSALPASTSLSFGNLLSPSQTAAPAASSIKGGGDAKLLSASEKTAENEAPESSPPTTQTPQPQIQLSEPVKEPPASQTAAGDTSTVGSSTASVAPPPDAIATPASTSELKAPVPPAASASVPQDQSISEAASATTIASPAEAASTLTAAAGVPTCVQSPAVGASVFVATSAGSSVFSQPPNSSSSGSAFSQPAGDTTATPSTPSVFGQLPAGTTASAPFGQPTTATVTTPTASTGFGTSAFGTTTTGGFGQPAFGQAPTFGQPASSSSSGFTFNQSGFGTVPAFGQPAASTAASSSGNVFGASSSTNSASSFSFGQPSASTGGGMFGQSNTLVFGQSSSFGQGGSVFGNTAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGGIVFGQQSSSSTGGLFGSGSGGRGGGFFTGLGGKPSQDAANKNPFGSSSGGFGSAASQNSSSLFGNSGAKAFGFGNSSFGDQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFSTLANQNTPTFGSLSQQGTGFGTQNSGFSAFGTVGGGFGFGSTNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_015279736.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279736.1

ORF Insert Sequence:

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGTGCCTAGC 
TCTACAGGCA GTGAACTTCC ACCCTACCCC TTTGCCACCG ATACCTCCAA GCAAGTCCTG 
GCTCCAAGTC TTACAGGAGC GGCAACATTC TCTTTCTCCT CTGCTTCTCC TAAGGCATCC 
ATAACACCAG CTCCTTCTGT GGGCACTACT GCAGCCACTG CCACATCCTT CTCCTTTGGA 
TCCATTAACT TGAAACCATC TGCAGAAAGT TCACCTGGGC TGCCACACTC CACAGCTCCT 
GCTGCACAGA AGTCATCTTT TGCCCCTTCG GCTTCTTCAG TTAAAGTGAA TCTCAATGAG 
AAGTTCACTG CTGTAGATAC TTCATCTCCG ACTACCAGCA GCGGTCCTAC CAGTTCCACA 
ATGTTTTCAT TCTCAACCAC TTCAAAAACT GTTTCTGGAG GTCCTCTGAG TCAGCCTGTG 
ACTCTCTCAG CCTCTCCTAC CACACCACTG AAGACTTCAA CCCCTGCACC TGCTGCAATG 
GCGCGTCCTG CTCAAAACGT GCCTGCAGCT TCCTTACTAC AGAAGACAGC CAAAACCACC 
CCTTCTGCAG CAAAACCAGG CTCTACTCAG GGCAAATCAG CACTCTCCAG CGCTGCTTTA 
GACAAACCTG CCCACCAGTG GAAGGATTCA GACCCTGTCA TCGCAGGAAT CAGAGAGGAG 
ATTGCACATT TTCAAAAGGA AATGGAGGAG CTGAAGGCCC GGACTTCCAA AGCCTGTTTC 
CAAGTGGGTA CTCCAGAGGA AATGAAAACG TTGCGAACTG AGTCAGATGA TCTCCACACC 
TTCCTCTTGG AAATTAAAGA AACAACCGAG TCGCTGCATG GAGATATTAG TACTCTGAAG 
ACAACCTTGC TGGAAGGATT TGCAGGGGTA GAAGAGGCTA GAGAGCAGAA TGAGAGGAAT 
CATAGCTTGG GGTATTTGCA CTTACTTTAT AAGAAACCTT TGGATCCCAA GAGTGAAGCA 
CAACTTCAGG AGATTCGACG CCTGCACCAG TATGTAAAGT TTGCTGTTCA GGATGTGAAT 
GATGTTCTGG ATTTGGAATG GGATCGGCAC CTGGAACAGG AGAGAAAGCA GAAGCGTCTG 
ATTGTTCCTG AACGAGAGAC TTTGTTTAAC ACCCTTGCTA ATAACCGGGA AATAATCAAT 
CAGCAGAAGA AGAGACTAAA TCAGTTGGTG AACAGCCTAC AGCAGCTCCG ACTCTATAAT 
CAAACATCTC AGTGGAGTGT TCCCAGTGAT ACATCCTCTC CTAGCAGTAC TCAAAGTTTT 
GACAGTGACC TTGAAAGCCT GCGCAGTGCC TTGATGAAAA CTACCTTGGA ATCACGTGCC 
AAACCCCCAC CTAAAGCACC TGCTAAGTTG TCCCCTGTGA AGCTGACACA GTTGAGGAAT 
TTTCTAGCCA AGAGGAAGAC TCCACCAGTT AGGTCTATGG CTCCAGCAAG TTTGTCCCGA 
TCTGCCTTCT TGTCCACAAG ATACTATGAA GACTTGGATG AAGTCAGTTC AACATCCTCA 
GTTTCTCAGT CTCTGGAGAA TGAGGATCTG CAGGCAGCGG ACCAGGAAGA GGAGGCTGCT 
CCCATCCAAG TCCCTCGTCA TGCCCCTGTG GTCCGCACTC CGTCTATCCA GCCTGGTTTG 
GTGGCTCAGC CTCCATCCTT TGGGAAACAA CACCCCTCCT TGGGCACTCT ATTGCCTACA 
TCTTCGAACA GAATCATCCC ACAAGGGGCA GACAGCACAA TGCTTGCAAC AAAGACTGTG 
AAACATGGGG CCCCTGCTCC CACCGTCACT GTTCCAGCAC CCCAGGCAGC TGCTGCGGCA 
GCCCTGCGGA GGCAGATGGC CACCCAGTCC CCAGCTGTGA GCACCTCCTT GACTGAATCA 
ACTCTGAAGA ACGTTCCCCA GGTGGTGAAT GTGCGAGAAC TCAAGACTGG TGCGTTGACT 
CCAGCTGTCT CTACAGTAAT AGGGTCATCA GTTCCTCACT CTGCAGCCCA GGCAGTCCAC 
CAAGTTCTGG CAAGTGTTGC TACAAATCAG GCTAAGCAGA CACCTCAAAC AAGCTCACTT 
AAGGGACAGG CTCCACCTTC TTCTGTATCT GCATCTTGTC CTGTGGCATC TACACCTGGT 
CCCACATCTG GCAACAAAAT CTCAGGACAA GGGACAGCAA AAACTGTTAC AGCTGTGACC 
TCTACAGTGG CGTCAGTACC AGCAACATCT GCACAGCCTA ACAAGGCTTT CTCATTTTCT 
TCTGTGGGGT CTGGGTTTAA TTTTGGTGTT GTCACGTCGG CTGCTTCATC ACCGACACTG 
CCCAGCCTTG CTGCCTCACA AGCCCCTGCT AAGGAGTCCA CTCAACCTGA TTCATTTTCG 
CTTGCTGGGG GTTCCAAATT TGGAGCAGTC CCTGAGAGCT CCTTTGCTTT TGGCTCCCTC 
AAGCCAGCAA GTGCTTCTGG AGCCGCAGTA GCAAATAGCT CTTCAGCAGA TGGCACTCAG 
TCAAACAAAC CTGCTGCAAC AATGTCTTCT GCTCCATCCA CAGCCTCTGG CAAATTAGAT 
ATCCCTCAGT CTAAGCCAGG GGACACCTTG TTTCTGAGCA GTCTGTCCGG GGAAACTCTC 
GGGAGCTTCT CAGGACTGCG TGTTGGTCAA GCAGATGATA ATGCCAAAAC CACCACTAAA 
ACACCATCTA CCAGTGCTGC AGGAGCACAG TCAACTAAAG CATCTACATC ACCTTCTGGG 
TTTGTCTTTG GTAGCCCCCT GATGTTAGGA AAAGCTGGAG AAGCTACTTC AACATCAGTC 
ACGTCAGCTG GCACGGCACC CACCTCCATC AGCACCAGTG CCACAGGCAG TCTCTTTGGC 
AATGTTCAGA TAACCAACAC AGGCTCTCCT GGAGTATTTA ATCTCGCAGG CTCTAGTGGA 
AACAAACTCA ACTTTTCTTT TGGTATTCAG CAAGCAAGCA GCATGAGCAC GTCCACTTCT 
GCCCCTTCTG CTCTCCCTGC TTCAACATCT CTCTCGTTCG GGAATCTCTT GAGTCCGTCA 
CAAACTGCTG CCCCGGCTGC AAGTTCTATT AAAGGTGGAG GAGATGCCAA ACTGCTGTCT 
GCTTCAGAAA AGACAGCTGA AAATGAAGCA CCTGAGTCTT CACCTCCAAC AACGCAGACT 
CCACAGCCAC AGATACAGCT TTCAGAGCCC GTTAAAGAGC CCCCTGCATC CCAGACAGCA 
GCTGGGGACA CCAGCACCGT GGGCTCCAGT ACTGCATCAG TAGCACCTCC CCCTGATGCC 
ATTGCTACTC CTGCTTCCAC AAGTGAGCTG AAGGCACCAG TGCCTCCTGC TGCCTCAGCC 
TCTGTCCCAC AGGATCAGAG CATCTCAGAG GCGGCCTCTG CAACAACCAT TGCCTCTCCA 
GCAGAAGCAG CGAGCACACT GACTGCTGCC GCTGGTGTTC CTACATGTGT TCAGAGTCCA 
GCTGTTGGTG CCTCGGTGTT TGTCGCCACC TCTGCAGGCT CCTCAGTGTT CTCTCAGCCT 
CCAAATTCCA GCTCTTCTGG CTCAGCATTT AGCCAGCCTG CAGGTGATAC AACTGCCACT 
CCTTCCACAC CGTCTGTTTT TGGCCAACTG CCTGCAGGTA CCACAGCATC AGCTCCATTT 
GGACAACCCA CCACTGCCAC CGTGACCACA CCGACTGCCA GCACAGGGTT TGGTACTTCT 
GCCTTTGGCA CCACCACGAC TGGGGGCTTT GGTCAGCCAG CCTTTGGCCA AGCACCGACC 
TTTGGGCAGC CAGCAAGCAG TTCTTCAAGT GGTTTTACCT TCAACCAGTC TGGATTTGGG 
ACAGTGCCTG CCTTTGGCCA GCCAGCAGCT TCCACTGCAG CCTCAAGCAG CGGCAATGTA 
TTTGGAGCAT CTTCTAGCAC CAACAGTGCA AGCTCCTTCT CCTTCGGACA GCCATCTGCC 
AGCACTGGGG GCGGGATGTT TGGTCAAAGC AACACTCTCG TGTTTGGGCA AAGCAGCAGC 
TTTGGGCAAG GGGGATCTGT CTTTGGTAAC ACCGCCGCTG TGACTACCAC TACATCATCT 
GCTGGGTTCA GCTTCTGCCA GGCCTCAGGT TTTGGTTCTA GCAACACAGG TTCTGTGTTT 
GGGCAAGCAG CAAATACTGG AGGCATTGTC TTTGGGCAGC AGTCGTCATC TTCCACTGGA 
GGCCTCTTTG GATCTGGAAG TGGTGGGAGA GGTGGAGGCT TCTTCACTGG TTTGGGAGGA 
AAGCCAAGTC AGGATGCAGC CAATAAGAAT CCTTTTGGTT CCTCCAGTGG AGGATTTGGA 
TCGGCAGCTT CCCAGAATTC TTCTAGCTTA TTTGGGAACA GTGGAGCCAA GGCCTTTGGA 
TTTGGCAACT CATCTTTTGG AGACCAGAAA CCCACAGGCA CTTTCAGCTC TGGTGGTGGC 
AGTGTGGCAT CTCAAGGCTT TGGGTTCTCT AGTCCAACCA AAGCAGGTGG CTTCGGTGCT 
GCTCCAGTGT TTGGCAGCCC TCCCACTTTT GGGGGATCCC CTGGGTTTGG AGGGGTGCCA 
GCATTCGGTT CAGCCCCAAC CTTTTCAAGC CCTCTGGGCT CGACGGGAGG CAAAGTGTTC 
GGCGAGGGTA CAGCTGCAGC CAGCGCCGGA GGATTTGGGT TTGGTGGTAC CAGTAACAAC 
ACAGCATCAT TTAGCACGCT AGCAAATCAG AATACCCCGA CGTTTGGATC GCTGTCACAG 
CAGGGTACTG GTTTTGGCAC ACAGAACAGT GGATTCTCTG CTTTCGGGAC AGTTGGAGGA 
GGGTTTGGTT TTGGATCAAC TAACACATCT TCCTCTGGAT TTAGCGGATG GAGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_015279737.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135223.1 Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

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CloneID	OGa43656
Clone ID Related Accession (Same CDS sequence)	XM_015279737.1
Accession Version	XM_015279737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGTGCCTAGC 
TCTACAGGCA GTGAACTTCC ACCCTACCCC TTTGCCACCG ATACCTCCAA GCAAGTCCTG 
GCTCCAAGTC TTACAGGAGC GGCAACATTC TCTTTCTCCT CTGCTTCTCC TAAGGCATCC 
ATAACACCAG CTCCTTCTGT GGGCACTACT GCAGCCACTG CCACATCCTT CTCCTTTGGA 
TCCATTAACT TGAAACCATC TGCAGAAAGT TCACCTGGGC TGCCACACTC CACAGCTCCT 
GCTGCACAGA AGTCATCTTT TGCCCCTTCG GCTTCTTCAG TTAAAGTGAA TCTCAATGAG 
AAGTTCACTG CTGTAGATAC TTCATCTCCG ACTACCAGCA GCGGTCCTAC CAGTTCCACA 
ATGTTTTCAT TCTCAACCAC TTCAAAAACT GTTTCTGGAG GTCCTCTGAG TCAGCCTGTG 
ACTCTCTCAG CCTCTCCTAC CACACCACTG AAGACTTCAA CCCCTGCACC TGCTGCAATG 
GCGCGTCCTG CTCAAAACGT GCCTGCAGCT TCCTTACTAC AGAAGACAGC CAAAACCACC 
CCTTCTGCAG CAAAACCAGG CTCTACTCAG GGCAAATCAG CACTCTCCAG CGCTGCTTTA 
GACAAACCTG CCCACCAGTG GAAGGATTCA GACCCTGTCA TCGCAGGAAT CAGAGAGGAG 
ATTGCACATT TTCAAAAGGA AATGGAGGAG CTGAAGGCCC GGACTTCCAA AGCCTGTTTC 
CAAGTGGGTA CTCCAGAGGA AATGAAAACG TTGCGAACTG AGTCAGATGA TCTCCACACC 
TTCCTCTTGG AAATTAAAGA AACAACCGAG TCGCTGCATG GAGATATTAG TACTCTGAAG 
ACAACCTTGC TGGAAGGATT TGCAGGGGTA GAAGAGGCTA GAGAGCAGAA TGAGAGGAAT 
CATAGCTTGG GGTATTTGCA CTTACTTTAT AAGAAACCTT TGGATCCCAA GAGTGAAGCA 
CAACTTCAGG AGATTCGACG CCTGCACCAG TATGTAAAGT TTGCTGTTCA GGATGTGAAT 
GATGTTCTGG ATTTGGAATG GGATCGGCAC CTGGAACAGG AGAGAAAGCA GAAGCGTCTG 
ATTGTTCCTG AACGAGAGAC TTTGTTTAAC ACCCTTGCTA ATAACCGGGA AATAATCAAT 
CAGCAGAAGA AGAGACTAAA TCAGTTGGTG AACAGCCTAC AGCAGCTCCG ACTCTATAAT 
CAAACATCTC AGTGGAGTGT TCCCAGTGAT ACATCCTCTC CTAGCAGTAC TCAAAGTTTT 
GACAGTGACC TTGAAAGCCT GCGCAGTGCC TTGATGAAAA CTACCTTGGA ATCACGTGCC 
AAACCCCCAC CTAAAGCACC TGCTAAGTTG TCCCCTGTGA AGCTGACACA GTTGAGGAAT 
TTTCTAGCCA AGAGGAAGAC TCCACCAGTT AGGTCTATGG CTCCAGCAAG TTTGTCCCGA 
TCTGCCTTCT TGTCCACAAG ATACTATGAA GACTTGGATG AAGTCAGTTC AACATCCTCA 
GTTTCTCAGT CTCTGGAGAA TGAGGATCTG CAGGCAGCGG ACCAGGAAGA GGAGGCTGCT 
CCCATCCAAG TCCCTCGTCA TGCCCCTGTG GTCCGCACTC CGTCTATCCA GCCTGGTTTG 
GTGGCTCAGC CTCCATCCTT TGGGAAACAA CACCCCTCCT TGGGCACTCT ATTGCCTACA 
TCTTCGAACA GAATCATCCC ACAAGGGGCA GACAGCACAA TGCTTGCAAC AAAGACTGTG 
AAACATGGGG CCCCTGCTCC CACCGTCACT GTTCCAGCAC CCCAGGCAGC TGCTGCGGCA 
GCCCTGCGGA GGCAGATGGC CACCCAGTCC CCAGCTGTGA GCACCTCCTT GACTGAATCA 
ACTCTGAAGA ACGTTCCCCA GGTGGTGAAT GTGCGAGAAC TCAAGACTGG TGCGTTGACT 
CCAGCTGTCT CTACAGTAAT AGGGTCATCA GTTCCTCACT CTGCAGCCCA GGCAGTCCAC 
CAAGTTCTGG CAAGTGTTGC TACAAATCAG GCTAAGCAGA CACCTCAAAC AAGCTCACTT 
AAGGGACAGG CTCCACCTTC TTCTGTATCT GCATCTTGTC CTGTGGCATC TACACCTGGT 
CCCACATCTG GCAACAAAAT CTCAGGACAA GGGACAGCAA AAACTGTTAC AGCTGTGACC 
TCTACAGTGG CGTCAGTACC AGCAACATCT GCACAGCCTA ACAAGGCTTT CTCATTTTCT 
TCTGTGGGGT CTGGGTTTAA TTTTGGTGTT GTCACGTCGG CTGCTTCATC ACCGACACTG 
CCCAGCCTTG CTGCCTCACA AGCCCCTGCT AAGGAGTCCA CTCAACCTGA TTCATTTTCG 
CTTGCTGGGG GTTCCAAATT TGGAGCAGTC CCTGAGAGCT CCTTTGCTTT TGGCTCCCTC 
AAGCCAGCAA GTGCTTCTGG AGCCGCAGTA GCAAATAGCT CTTCAGCAGA TGGCACTCAG 
TCAAACAAAC CTGCTGCAAC AATGTCTTCT GCTCCATCCA CAGCCTCTGG CAAATTAGAT 
ATCCCTCAGT CTAAGCCAGG GGACACCTTG TTTCTGAGCA GTCTGTCCGG GGAAACTCTC 
GGGAGCTTCT CAGGACTGCG TGTTGGTCAA GCAGATGATA ATGCCAAAAC CACCACTAAA 
ACACCATCTA CCAGTGCTGC AGGAGCACAG TCAACTAAAG CATCTACATC ACCTTCTGGG 
TTTGTCTTTG GTAGCCCCCT GATGTTAGGA AAAGCTGGAG AAGCTACTTC AACATCAGTC 
ACGTCAGCTG GCACGGCACC CACCTCCATC AGCACCAGTG CCACAGGCAG TCTCTTTGGC 
AATGTTCAGA TAACCAACAC AGGCTCTCCT GGAGTATTTA ATCTCGCAGG CTCTAGTGGA 
AACAAACTCA ACTTTTCTTT TGGTATTCAG CAAGCAAGCA GCATGAGCAC GTCCACTTCT 
GCCCCTTCTG CTCTCCCTGC TTCAACATCT CTCTCGTTCG GGAATCTCTT GAGTCCGTCA 
CAAACTGCTG CCCCGGCTGC AAGTTCTATT AAAGGTGGAG GAGATGCCAA ACTGCTGTCT 
GCTTCAGAAA AGACAGCTGA AAATGAAGCA CCTGAGTCTT CACCTCCAAC AACGCAGACT 
CCACAGCCAC AGATACAGCT TTCAGAGCCC GTTAAAGAGC CCCCTGCATC CCAGACAGCA 
GCTGGGGACA CCAGCACCGT GGGCTCCAGT ACTGCATCAG TAGCACCTCC CCCTGATGCC 
ATTGCTACTC CTGCTTCCAC AAGTGAGCTG AAGGCACCAG TGCCTCCTGC TGCCTCAGCC 
TCTGTCCCAC AGGATCAGAG CATCTCAGAG GCGGCCTCTG CAACAACCAT TGCCTCTCCA 
GCAGAAGCAG CGAGCACACT GACTGCTGCC GCTGGTGTTC CTACATGTGT TCAGAGTCCA 
GCTGTTGGTG CCTCGGTGTT TGTCGCCACC TCTGCAGGCT CCTCAGTGTT CTCTCAGCCT 
CCAAATTCCA GCTCTTCTGG CTCAGCATTT AGCCAGCCTG CAGGTGATAC AACTGCCACT 
CCTTCCACAC CGTCTGTTTT TGGCCAACTG CCTGCAGGTA CCACAGCATC AGCTCCATTT 
GGACAACCCA CCACTGCCAC CGTGACCACA CCGACTGCCA GCACAGGGTT TGGTACTTCT 
GCCTTTGGCA CCACCACGAC TGGGGGCTTT GGTCAGCCAG CCTTTGGCCA AGCACCGACC 
TTTGGGCAGC CAGCAAGCAG TTCTTCAAGT GGTTTTACCT TCAACCAGTC TGGATTTGGG 
ACAGTGCCTG CCTTTGGCCA GCCAGCAGCT TCCACTGCAG CCTCAAGCAG CGGCAATGTA 
TTTGGAGCAT CTTCTAGCAC CAACAGTGCA AGCTCCTTCT CCTTCGGACA GCCATCTGCC 
AGCACTGGGG GCGGGATGTT TGGTCAAAGC AACACTCTCG TGTTTGGGCA AAGCAGCAGC 
TTTGGGCAAG GGGGATCTGT CTTTGGTAAC ACCGCCGCTG TGACTACCAC TACATCATCT 
GCTGGGTTCA GCTTCTGCCA GGCCTCAGGT TTTGGTTCTA GCAACACAGG TTCTGTGTTT 
GGGCAAGCAG CAAATACTGG AGGCATTGTC TTTGGGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135223.1
CDS	116..6352
Translation	MGEDGDLVPEREMKDFQFRALKKVRIFDPPDDLPKERSSLLVVSNKYGLVFAGGGTSLQIFHTRDLLMQNQLGEDPNNIVENVQGTSVPMKFPVQHLALSCDNLTLSVCMMSSDIGSFIGFFDVRTFFNQAKQQKRMFAYHKLAKDSGNAAMVNDLKWNPASPTMVAVCLSDGSISVLQVTDSVKVYATLPPSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPNLQEKKVIPYPPFYQADNPVKVLDVLWIGTYVFAIVYAAADGTLETPPEVVVAILPKKEEKRPEKFVNFMEPCYGSCAERQHHYYLNYVEEWDLILAASAASTEVSILARQGDQNWESWILEDSSRAELPVTDKSDDTLPVGVAVDYTNNMPIPISDEKTLPPAPVLILLSTDGVLCPFYMINQNPGVRSLLVTPEPLPAAGERAPKSPGSTVTAPTTPPTSLKPDAVPAVAPAPSQPPAGDGAAAFSFTPSTAKVPSSTGSELPPYPFATDTSKQVLAPSLTGAATFSFSSASPKASITPAPSVGTTAATATSFSFGSINLKPSAESSPGLPHSTAPAAQKSSFAPSASSVKVNLNEKFTAVDTSSPTTSSGPTSSTMFSFSTTSKTVSGGPLSQPVTLSASPTTPLKTSTPAPAAMARPAQNVPAASLLQKTAKTTPSAAKPGSTQGKSALSSAALDKPAHQWKDSDPVIAGIREEIAHFQKEMEELKARTSKACFQVGTPEEMKTLRTESDDLHTFLLEIKETTESLHGDISTLKTTLLEGFAGVEEAREQNERNHSLGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQERKQKRLIVPERETLFNTLANNREIINQQKKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSPSSTQSFDSDLESLRSALMKTTLESRAKPPPKAPAKLSPVKLTQLRNFLAKRKTPPVRSMAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENEDLQAADQEEEAAPIQVPRHAPVVRTPSIQPGLVAQPPSFGKQHPSLGTLLPTSSNRIIPQGADSTMLATKTVKHGAPAPTVTVPAPQAAAAAALRRQMATQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSTVIGSSVPHSAAQAVHQVLASVATNQAKQTPQTSSLKGQAPPSSVSASCPVASTPGPTSGNKISGQGTAKTVTAVTSTVASVPATSAQPNKAFSFSSVGSGFNFGVVTSAASSPTLPSLAASQAPAKESTQPDSFSLAGGSKFGAVPESSFAFGSLKPASASGAAVANSSSADGTQSNKPAATMSSAPSTASGKLDIPQSKPGDTLFLSSLSGETLGSFSGLRVGQADDNAKTTTKTPSTSAAGAQSTKASTSPSGFVFGSPLMLGKAGEATSTSVTSAGTAPTSISTSATGSLFGNVQITNTGSPGVFNLAGSSGNKLNFSFGIQQASSMSTSTSAPSALPASTSLSFGNLLSPSQTAAPAASSIKGGGDAKLLSASEKTAENEAPESSPPTTQTPQPQIQLSEPVKEPPASQTAAGDTSTVGSSTASVAPPPDAIATPASTSELKAPVPPAASASVPQDQSISEAASATTIASPAEAASTLTAAAGVPTCVQSPAVGASVFVATSAGSSVFSQPPNSSSSGSAFSQPAGDTTATPSTPSVFGQLPAGTTASAPFGQPTTATVTTPTASTGFGTSAFGTTTTGGFGQPAFGQAPTFGQPASSSSSGFTFNQSGFGTVPAFGQPAASTAASSSGNVFGASSSTNSASSFSFGQPSASTGGGMFGQSNTLVFGQSSSFGQGGSVFGNTAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGGIVFGQSSSSTGGLFGSGSGGRGGGFFTGLGGKPSQDAANKNPFGSSSGGFGSAASQNSSSLFGNSGAKAFGFGNSSFGDQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFSTLANQNTPTFGSLSQQGTGFGTQNSGFSAFGTVGGGFGFGSTNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_015279737.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279737.1

ORF Insert Sequence:

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGTGCCTAGC 
TCTACAGGCA GTGAACTTCC ACCCTACCCC TTTGCCACCG ATACCTCCAA GCAAGTCCTG 
GCTCCAAGTC TTACAGGAGC GGCAACATTC TCTTTCTCCT CTGCTTCTCC TAAGGCATCC 
ATAACACCAG CTCCTTCTGT GGGCACTACT GCAGCCACTG CCACATCCTT CTCCTTTGGA 
TCCATTAACT TGAAACCATC TGCAGAAAGT TCACCTGGGC TGCCACACTC CACAGCTCCT 
GCTGCACAGA AGTCATCTTT TGCCCCTTCG GCTTCTTCAG TTAAAGTGAA TCTCAATGAG 
AAGTTCACTG CTGTAGATAC TTCATCTCCG ACTACCAGCA GCGGTCCTAC CAGTTCCACA 
ATGTTTTCAT TCTCAACCAC TTCAAAAACT GTTTCTGGAG GTCCTCTGAG TCAGCCTGTG 
ACTCTCTCAG CCTCTCCTAC CACACCACTG AAGACTTCAA CCCCTGCACC TGCTGCAATG 
GCGCGTCCTG CTCAAAACGT GCCTGCAGCT TCCTTACTAC AGAAGACAGC CAAAACCACC 
CCTTCTGCAG CAAAACCAGG CTCTACTCAG GGCAAATCAG CACTCTCCAG CGCTGCTTTA 
GACAAACCTG CCCACCAGTG GAAGGATTCA GACCCTGTCA TCGCAGGAAT CAGAGAGGAG 
ATTGCACATT TTCAAAAGGA AATGGAGGAG CTGAAGGCCC GGACTTCCAA AGCCTGTTTC 
CAAGTGGGTA CTCCAGAGGA AATGAAAACG TTGCGAACTG AGTCAGATGA TCTCCACACC 
TTCCTCTTGG AAATTAAAGA AACAACCGAG TCGCTGCATG GAGATATTAG TACTCTGAAG 
ACAACCTTGC TGGAAGGATT TGCAGGGGTA GAAGAGGCTA GAGAGCAGAA TGAGAGGAAT 
CATAGCTTGG GGTATTTGCA CTTACTTTAT AAGAAACCTT TGGATCCCAA GAGTGAAGCA 
CAACTTCAGG AGATTCGACG CCTGCACCAG TATGTAAAGT TTGCTGTTCA GGATGTGAAT 
GATGTTCTGG ATTTGGAATG GGATCGGCAC CTGGAACAGG AGAGAAAGCA GAAGCGTCTG 
ATTGTTCCTG AACGAGAGAC TTTGTTTAAC ACCCTTGCTA ATAACCGGGA AATAATCAAT 
CAGCAGAAGA AGAGACTAAA TCAGTTGGTG AACAGCCTAC AGCAGCTCCG ACTCTATAAT 
CAAACATCTC AGTGGAGTGT TCCCAGTGAT ACATCCTCTC CTAGCAGTAC TCAAAGTTTT 
GACAGTGACC TTGAAAGCCT GCGCAGTGCC TTGATGAAAA CTACCTTGGA ATCACGTGCC 
AAACCCCCAC CTAAAGCACC TGCTAAGTTG TCCCCTGTGA AGCTGACACA GTTGAGGAAT 
TTTCTAGCCA AGAGGAAGAC TCCACCAGTT AGGTCTATGG CTCCAGCAAG TTTGTCCCGA 
TCTGCCTTCT TGTCCACAAG ATACTATGAA GACTTGGATG AAGTCAGTTC AACATCCTCA 
GTTTCTCAGT CTCTGGAGAA TGAGGATCTG CAGGCAGCGG ACCAGGAAGA GGAGGCTGCT 
CCCATCCAAG TCCCTCGTCA TGCCCCTGTG GTCCGCACTC CGTCTATCCA GCCTGGTTTG 
GTGGCTCAGC CTCCATCCTT TGGGAAACAA CACCCCTCCT TGGGCACTCT ATTGCCTACA 
TCTTCGAACA GAATCATCCC ACAAGGGGCA GACAGCACAA TGCTTGCAAC AAAGACTGTG 
AAACATGGGG CCCCTGCTCC CACCGTCACT GTTCCAGCAC CCCAGGCAGC TGCTGCGGCA 
GCCCTGCGGA GGCAGATGGC CACCCAGTCC CCAGCTGTGA GCACCTCCTT GACTGAATCA 
ACTCTGAAGA ACGTTCCCCA GGTGGTGAAT GTGCGAGAAC TCAAGACTGG TGCGTTGACT 
CCAGCTGTCT CTACAGTAAT AGGGTCATCA GTTCCTCACT CTGCAGCCCA GGCAGTCCAC 
CAAGTTCTGG CAAGTGTTGC TACAAATCAG GCTAAGCAGA CACCTCAAAC AAGCTCACTT 
AAGGGACAGG CTCCACCTTC TTCTGTATCT GCATCTTGTC CTGTGGCATC TACACCTGGT 
CCCACATCTG GCAACAAAAT CTCAGGACAA GGGACAGCAA AAACTGTTAC AGCTGTGACC 
TCTACAGTGG CGTCAGTACC AGCAACATCT GCACAGCCTA ACAAGGCTTT CTCATTTTCT 
TCTGTGGGGT CTGGGTTTAA TTTTGGTGTT GTCACGTCGG CTGCTTCATC ACCGACACTG 
CCCAGCCTTG CTGCCTCACA AGCCCCTGCT AAGGAGTCCA CTCAACCTGA TTCATTTTCG 
CTTGCTGGGG GTTCCAAATT TGGAGCAGTC CCTGAGAGCT CCTTTGCTTT TGGCTCCCTC 
AAGCCAGCAA GTGCTTCTGG AGCCGCAGTA GCAAATAGCT CTTCAGCAGA TGGCACTCAG 
TCAAACAAAC CTGCTGCAAC AATGTCTTCT GCTCCATCCA CAGCCTCTGG CAAATTAGAT 
ATCCCTCAGT CTAAGCCAGG GGACACCTTG TTTCTGAGCA GTCTGTCCGG GGAAACTCTC 
GGGAGCTTCT CAGGACTGCG TGTTGGTCAA GCAGATGATA ATGCCAAAAC CACCACTAAA 
ACACCATCTA CCAGTGCTGC AGGAGCACAG TCAACTAAAG CATCTACATC ACCTTCTGGG 
TTTGTCTTTG GTAGCCCCCT GATGTTAGGA AAAGCTGGAG AAGCTACTTC AACATCAGTC 
ACGTCAGCTG GCACGGCACC CACCTCCATC AGCACCAGTG CCACAGGCAG TCTCTTTGGC 
AATGTTCAGA TAACCAACAC AGGCTCTCCT GGAGTATTTA ATCTCGCAGG CTCTAGTGGA 
AACAAACTCA ACTTTTCTTT TGGTATTCAG CAAGCAAGCA GCATGAGCAC GTCCACTTCT 
GCCCCTTCTG CTCTCCCTGC TTCAACATCT CTCTCGTTCG GGAATCTCTT GAGTCCGTCA 
CAAACTGCTG CCCCGGCTGC AAGTTCTATT AAAGGTGGAG GAGATGCCAA ACTGCTGTCT 
GCTTCAGAAA AGACAGCTGA AAATGAAGCA CCTGAGTCTT CACCTCCAAC AACGCAGACT 
CCACAGCCAC AGATACAGCT TTCAGAGCCC GTTAAAGAGC CCCCTGCATC CCAGACAGCA 
GCTGGGGACA CCAGCACCGT GGGCTCCAGT ACTGCATCAG TAGCACCTCC CCCTGATGCC 
ATTGCTACTC CTGCTTCCAC AAGTGAGCTG AAGGCACCAG TGCCTCCTGC TGCCTCAGCC 
TCTGTCCCAC AGGATCAGAG CATCTCAGAG GCGGCCTCTG CAACAACCAT TGCCTCTCCA 
GCAGAAGCAG CGAGCACACT GACTGCTGCC GCTGGTGTTC CTACATGTGT TCAGAGTCCA 
GCTGTTGGTG CCTCGGTGTT TGTCGCCACC TCTGCAGGCT CCTCAGTGTT CTCTCAGCCT 
CCAAATTCCA GCTCTTCTGG CTCAGCATTT AGCCAGCCTG CAGGTGATAC AACTGCCACT 
CCTTCCACAC CGTCTGTTTT TGGCCAACTG CCTGCAGGTA CCACAGCATC AGCTCCATTT 
GGACAACCCA CCACTGCCAC CGTGACCACA CCGACTGCCA GCACAGGGTT TGGTACTTCT 
GCCTTTGGCA CCACCACGAC TGGGGGCTTT GGTCAGCCAG CCTTTGGCCA AGCACCGACC 
TTTGGGCAGC CAGCAAGCAG TTCTTCAAGT GGTTTTACCT TCAACCAGTC TGGATTTGGG 
ACAGTGCCTG CCTTTGGCCA GCCAGCAGCT TCCACTGCAG CCTCAAGCAG CGGCAATGTA 
TTTGGAGCAT CTTCTAGCAC CAACAGTGCA AGCTCCTTCT CCTTCGGACA GCCATCTGCC 
AGCACTGGGG GCGGGATGTT TGGTCAAAGC AACACTCTCG TGTTTGGGCA AAGCAGCAGC 
TTTGGGCAAG GGGGATCTGT CTTTGGTAAC ACCGCCGCTG TGACTACCAC TACATCATCT 
GCTGGGTTCA GCTTCTGCCA GGCCTCAGGT TTTGGTTCTA GCAACACAGG TTCTGTGTTT 
GGGCAAGCAG CAAATACTGG AGGCATTGTC TTTGGGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_015279738.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015135224.1 Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

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CloneID	OGa43657
Clone ID Related Accession (Same CDS sequence)	XM_015279738.1
Accession Version	XM_015279738.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764072.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGCATCCATA 
ACACCAGCTC CTTCTGTGGG CACTACTGCA GCCACTGCCA CATCCTTCTC CTTTGGATCC 
ATTAACTTGA AACCATCTGC AGAAAGTTCA CCTGGGCTGC CACACTCCAC AGCTCCTGCT 
GCACAGAAGT CATCTTTTGC CCCTTCGGCT TCTTCAGTTA AAGTGAATCT CAATGAGAAG 
TTCACTGCTG TAGATACTTC ATCTCCGACT ACCAGCAGCG GTCCTACCAG TTCCACAATG 
TTTTCATTCT CAACCACTTC AAAAACTGTT TCTGGAGGTC CTCTGAGTCA GCCTGTGACT 
CTCTCAGCCT CTCCTACCAC ACCACTGAAG ACTTCAACCC CTGCACCTGC TGCAATGGCG 
CGTCCTGCTC AAAACGTGCC TGCAGCTTCC TTACTACAGA AGACAGCCAA AACCACCCCT 
TCTGCAGCAA AACCAGGCTC TACTCAGGGC AAATCAGCAC TCTCCAGCGC TGCTTTAGAC 
AAACCTGCCC ACCAGTGGAA GGATTCAGAC CCTGTCATCG CAGGAATCAG AGAGGAGATT 
GCACATTTTC AAAAGGAAAT GGAGGAGCTG AAGGCCCGGA CTTCCAAAGC CTGTTTCCAA 
GTGGGTACTC CAGAGGAAAT GAAAACGTTG CGAACTGAGT CAGATGATCT CCACACCTTC 
CTCTTGGAAA TTAAAGAAAC AACCGAGTCG CTGCATGGAG ATATTAGTAC TCTGAAGACA 
ACCTTGCTGG AAGGATTTGC AGGGGTAGAA GAGGCTAGAG AGCAGAATGA GAGGAATCAT 
AGCTTGGGGT ATTTGCACTT ACTTTATAAG AAACCTTTGG ATCCCAAGAG TGAAGCACAA 
CTTCAGGAGA TTCGACGCCT GCACCAGTAT GTAAAGTTTG CTGTTCAGGA TGTGAATGAT 
GTTCTGGATT TGGAATGGGA TCGGCACCTG GAACAGGAGA GAAAGCAGAA GCGTCTGATT 
GTTCCTGAAC GAGAGACTTT GTTTAACACC CTTGCTAATA ACCGGGAAAT AATCAATCAG 
CAGAAGAAGA GACTAAATCA GTTGGTGAAC AGCCTACAGC AGCTCCGACT CTATAATCAA 
ACATCTCAGT GGAGTGTTCC CAGTGATACA TCCTCTCCTA GCAGTACTCA AAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAGTGCCTTG ATGAAAACTA CCTTGGAATC ACGTGCCAAA 
CCCCCACCTA AAGCACCTGC TAAGTTGTCC CCTGTGAAGC TGACACAGTT GAGGAATTTT 
CTAGCCAAGA GGAAGACTCC ACCAGTTAGG TCTATGGCTC CAGCAAGTTT GTCCCGATCT 
GCCTTCTTGT CCACAAGATA CTATGAAGAC TTGGATGAAG TCAGTTCAAC ATCCTCAGTT 
TCTCAGTCTC TGGAGAATGA GGATCTGCAG GCAGCGGACC AGGAAGAGGA GGCTGCTCCC 
ATCCAAGTCC CTCGTCATGC CCCTGTGGTC CGCACTCCGT CTATCCAGCC TGGTTTGGTG 
GCTCAGCCTC CATCCTTTGG GAAACAACAC CCCTCCTTGG GCACTCTATT GCCTACATCT 
TCGAACAGAA TCATCCCACA AGGGGCAGAC AGCACAATGC TTGCAACAAA GACTGTGAAA 
CATGGGGCCC CTGCTCCCAC CGTCACTGTT CCAGCACCCC AGGCAGCTGC TGCGGCAGCC 
CTGCGGAGGC AGATGGCCAC CCAGTCCCCA GCTGTGAGCA CCTCCTTGAC TGAATCAACT 
CTGAAGAACG TTCCCCAGGT GGTGAATGTG CGAGAACTCA AGACTGGTGC GTTGACTCCA 
GCTGTCTCTA CAGTAATAGG GTCATCAGTT CCTCACTCTG CAGCCCAGGC AGTCCACCAA 
GTTCTGGCAA GTGTTGCTAC AAATCAGGCT AAGCAGACAC CTCAAACAAG CTCACTTAAG 
GGACAGGCTC CACCTTCTTC TGTATCTGCA TCTTGTCCTG TGGCATCTAC ACCTGGTCCC 
ACATCTGGCA ACAAAATCTC AGGACAAGGG ACAGCAAAAA CTGTTACAGC TGTGACCTCT 
ACAGTGGCGT CAGTACCAGC AACATCTGCA CAGCCTAACA AGGCTTTCTC ATTTTCTTCT 
GTGGGGTCTG GGTTTAATTT TGGTGTTGTC ACGTCGGCTG CTTCATCACC GACACTGCCC 
AGCCTTGCTG CCTCACAAGC CCCTGCTAAG GAGTCCACTC AACCTGATTC ATTTTCGCTT 
GCTGGGGGTT CCAAATTTGG AGCAGTCCCT GAGAGCTCCT TTGCTTTTGG CTCCCTCAAG 
CCAGCAAGTG CTTCTGGAGC CGCAGTAGCA AATAGCTCTT CAGCAGATGG CACTCAGTCA 
AACAAACCTG CTGCAACAAT GTCTTCTGCT CCATCCACAG CCTCTGGCAA ATTAGATATC 
CCTCAGTCTA AGCCAGGGGA CACCTTGTTT CTGAGCAGTC TGTCCGGGGA AACTCTCGGG 
AGCTTCTCAG GACTGCGTGT TGGTCAAGCA GATGATAATG CCAAAACCAC CACTAAAACA 
CCATCTACCA GTGCTGCAGG AGCACAGTCA ACTAAAGCAT CTACATCACC TTCTGGGTTT 
GTCTTTGGTA GCCCCCTGAT GTTAGGAAAA GCTGGAGAAG CTACTTCAAC ATCAGTCACG 
TCAGCTGGCA CGGCACCCAC CTCCATCAGC ACCAGTGCCA CAGGCAGTCT CTTTGGCAAT 
GTTCAGATAA CCAACACAGG CTCTCCTGGA GTATTTAATC TCGCAGGCTC TAGTGGAAAC 
AAACTCAACT TTTCTTTTGG TATTCAGCAA GCAAGCAGCA TGAGCACGTC CACTTCTGCC 
CCTTCTGCTC TCCCTGCTTC AACATCTCTC TCGTTCGGGA ATCTCTTGAG TCCGTCACAA 
ACTGCTGCCC CGGCTGCAAG TTCTATTAAA GGTGGAGGAG ATGCCAAACT GCTGTCTGCT 
TCAGAAAAGA CAGCTGAAAA TGAAGCACCT GAGTCTTCAC CTCCAACAAC GCAGACTCCA 
CAGCCACAGA TACAGCTTTC AGAGCCCGTT AAAGAGCCCC CTGCATCCCA GACAGCAGCT 
GGGGACACCA GCACCGTGGG CTCCAGTACT GCATCAGTAG CACCTCCCCC TGATGCCATT 
GCTACTCCTG CTTCCACAAG TGAGCTGAAG GCACCAGTGC CTCCTGCTGC CTCAGCCTCT 
GTCCCACAGG ATCAGAGCAT CTCAGAGGCG GCCTCTGCAA CAACCATTGC CTCTCCAGCA 
GAAGCAGCGA GCACACTGAC TGCTGCCGCT GGTGTTCCTA CATGTGTTCA GAGTCCAGCT 
GTTGGTGCCT CGGTGTTTGT CGCCACCTCT GCAGGCTCCT CAGTGTTCTC TCAGCCTCCA 
AATTCCAGCT CTTCTGGCTC AGCATTTAGC CAGCCTGCAG GTGATACAAC TGCCACTCCT 
TCCACACCGT CTGTTTTTGG CCAACTGCCT GCAGGTACCA CAGCATCAGC TCCATTTGGA 
CAACCCACCA CTGCCACCGT GACCACACCG ACTGCCAGCA CAGGGTTTGG TACTTCTGCC 
TTTGGCACCA CCACGACTGG GGGCTTTGGT CAGCCAGCCT TTGGCCAAGC ACCGACCTTT 
GGGCAGCCAG CAAGCAGTTC TTCAAGTGGT TTTACCTTCA ACCAGTCTGG ATTTGGGACA 
GTGCCTGCCT TTGGCCAGCC AGCAGCTTCC ACTGCAGCCT CAAGCAGCGG CAATGTATTT 
GGAGCATCTT CTAGCACCAA CAGTGCAAGC TCCTTCTCCT TCGGACAGCC ATCTGCCAGC 
ACTGGGGGCG GGATGTTTGG TCAAAGCAAC ACTCTCGTGT TTGGGCAAAG CAGCAGCTTT 
GGGCAAGGGG GATCTGTCTT TGGTAACACC GCCGCTGTGA CTACCACTAC ATCATCTGCT 
GGGTTCAGCT TCTGCCAGGC CTCAGGTTTT GGTTCTAGCA ACACAGGTTC TGTGTTTGGG 
CAAGCAGCAA ATACTGGAGG CATTGTCTTT GGGCAGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015135224.1
CDS	116..6232
Translation	MGEDGDLVPEREMKDFQFRALKKVRIFDPPDDLPKERSSLLVVSNKYGLVFAGGGTSLQIFHTRDLLMQNQLGEDPNNIVENVQGTSVPMKFPVQHLALSCDNLTLSVCMMSSDIGSFIGFFDVRTFFNQAKQQKRMFAYHKLAKDSGNAAMVNDLKWNPASPTMVAVCLSDGSISVLQVTDSVKVYATLPPSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPNLQEKKVIPYPPFYQADNPVKVLDVLWIGTYVFAIVYAAADGTLETPPEVVVAILPKKEEKRPEKFVNFMEPCYGSCAERQHHYYLNYVEEWDLILAASAASTEVSILARQGDQNWESWILEDSSRAELPVTDKSDDTLPVGVAVDYTNNMPIPISDEKTLPPAPVLILLSTDGVLCPFYMINQNPGVRSLLVTPEPLPAAGERAPKSPGSTVTAPTTPPTSLKPDAVPAVAPAPSQPPAGDGAAAFSFTPSTAKASITPAPSVGTTAATATSFSFGSINLKPSAESSPGLPHSTAPAAQKSSFAPSASSVKVNLNEKFTAVDTSSPTTSSGPTSSTMFSFSTTSKTVSGGPLSQPVTLSASPTTPLKTSTPAPAAMARPAQNVPAASLLQKTAKTTPSAAKPGSTQGKSALSSAALDKPAHQWKDSDPVIAGIREEIAHFQKEMEELKARTSKACFQVGTPEEMKTLRTESDDLHTFLLEIKETTESLHGDISTLKTTLLEGFAGVEEAREQNERNHSLGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQERKQKRLIVPERETLFNTLANNREIINQQKKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSPSSTQSFDSDLESLRSALMKTTLESRAKPPPKAPAKLSPVKLTQLRNFLAKRKTPPVRSMAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENEDLQAADQEEEAAPIQVPRHAPVVRTPSIQPGLVAQPPSFGKQHPSLGTLLPTSSNRIIPQGADSTMLATKTVKHGAPAPTVTVPAPQAAAAAALRRQMATQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSTVIGSSVPHSAAQAVHQVLASVATNQAKQTPQTSSLKGQAPPSSVSASCPVASTPGPTSGNKISGQGTAKTVTAVTSTVASVPATSAQPNKAFSFSSVGSGFNFGVVTSAASSPTLPSLAASQAPAKESTQPDSFSLAGGSKFGAVPESSFAFGSLKPASASGAAVANSSSADGTQSNKPAATMSSAPSTASGKLDIPQSKPGDTLFLSSLSGETLGSFSGLRVGQADDNAKTTTKTPSTSAAGAQSTKASTSPSGFVFGSPLMLGKAGEATSTSVTSAGTAPTSISTSATGSLFGNVQITNTGSPGVFNLAGSSGNKLNFSFGIQQASSMSTSTSAPSALPASTSLSFGNLLSPSQTAAPAASSIKGGGDAKLLSASEKTAENEAPESSPPTTQTPQPQIQLSEPVKEPPASQTAAGDTSTVGSSTASVAPPPDAIATPASTSELKAPVPPAASASVPQDQSISEAASATTIASPAEAASTLTAAAGVPTCVQSPAVGASVFVATSAGSSVFSQPPNSSSSGSAFSQPAGDTTATPSTPSVFGQLPAGTTASAPFGQPTTATVTTPTASTGFGTSAFGTTTTGGFGQPAFGQAPTFGQPASSSSSGFTFNQSGFGTVPAFGQPAASTAASSSGNVFGASSSTNSASSFSFGQPSASTGGGMFGQSNTLVFGQSSSFGQGGSVFGNTAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGGIVFGQQSSSSTGGLFGSGSGGRGGGFFTGLGGKPSQDAANKNPFGSSSGGFGSAASQNSSSLFGNSGAKAFGFGNSSFGDQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFSTLANQNTPTFGSLSQQGTGFGTQNSGFSAFGTVGGGFGFGSTNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_015279738.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015279738.1

ORF Insert Sequence:

ATGGGGGAGG ACGGGGACTT GGTCCCCGAG CGGGAGATGA AGGATTTTCA GTTCAGAGCT 
CTGAAGAAGG TCCGCATCTT TGATCCTCCT GATGATCTTC CCAAAGAGCG GTCCAGCCTC 
CTTGTAGTGT CTAACAAATA TGGCCTGGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTTCACACTA GAGATCTTCT CATGCAAAAT CAGCTTGGAG AAGATCCCAA TAACATTGTG 
GAGAATGTGC AGGGTACCTC GGTTCCCATG AAGTTTCCAG TGCAGCACTT GGCGCTAAGC 
TGTGATAATC TCACGCTGTC TGTCTGTATG ATGTCCAGTG ACATTGGTTC CTTCATTGGC 
TTTTTTGATG TCCGCACATT CTTTAATCAG GCAAAACAGC AGAAGCGTAT GTTTGCTTAT 
CACAAGCTGG CCAAGGACTC AGGAAATGCA GCAATGGTCA ATGACCTGAA GTGGAACCCT 
GCTAGTCCCA CCATGGTGGC TGTCTGTCTG TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACATTG CCTCCCTCAG TAGCTGTTAC CTCTGTGTGT 
TGGAGCCCAA AGGGAAAACA ACTGGCTGTA GGGAGGCAAA ATGGCACTGT GGTGCAGTAT 
CTGCCTAATC TGCAAGAGAA GAAAGTAATC CCTTATCCTC CCTTTTATCA GGCAGACAAT 
CCTGTTAAAG TTTTGGATGT GCTGTGGATT GGCACATATG TCTTTGCTAT TGTATATGCA 
GCTGCTGATG GGACGCTGGA AACACCCCCA GAAGTGGTTG TGGCAATATT GCCTAAAAAG 
GAAGAGAAGC GACCAGAGAA GTTTGTGAAT TTCATGGAGC CTTGTTATGG AAGCTGTGCT 
GAAAGACAAC ATCATTACTA CCTCAACTAT GTTGAGGAAT GGGATCTAAT TCTGGCAGCT 
TCAGCTGCTT CCACAGAAGT CAGCATTCTT GCCAGGCAAG GAGATCAGAA CTGGGAATCA 
TGGATTTTGG AGGACTCCAG CAGAGCAGAA CTTCCTGTGA CAGATAAAAG TGATGATACT 
TTGCCTGTTG GTGTTGCTGT AGACTACACC AATAACATGC CTATCCCGAT CAGTGATGAG 
AAGACCCTTC CTCCAGCACC AGTTCTCATC TTGCTCTCTA CGGATGGTGT GCTCTGCCCA 
TTTTACATGA TCAATCAAAA TCCAGGGGTC AGATCGCTTC TTGTGACCCC AGAGCCATTG 
CCAGCAGCAG GGGAGAGAGC ACCAAAATCA CCAGGAAGTA CTGTCACAGC TCCGACCACT 
CCGCCAACTT CTTTAAAGCC TGATGCAGTA CCAGCTGTTG CTCCTGCCCC AAGTCAGCCT 
CCTGCAGGAG ATGGAGCAGC TGCTTTTTCC TTTACACCTT CAACTGCAAA GGCATCCATA 
ACACCAGCTC CTTCTGTGGG CACTACTGCA GCCACTGCCA CATCCTTCTC CTTTGGATCC 
ATTAACTTGA AACCATCTGC AGAAAGTTCA CCTGGGCTGC CACACTCCAC AGCTCCTGCT 
GCACAGAAGT CATCTTTTGC CCCTTCGGCT TCTTCAGTTA AAGTGAATCT CAATGAGAAG 
TTCACTGCTG TAGATACTTC ATCTCCGACT ACCAGCAGCG GTCCTACCAG TTCCACAATG 
TTTTCATTCT CAACCACTTC AAAAACTGTT TCTGGAGGTC CTCTGAGTCA GCCTGTGACT 
CTCTCAGCCT CTCCTACCAC ACCACTGAAG ACTTCAACCC CTGCACCTGC TGCAATGGCG 
CGTCCTGCTC AAAACGTGCC TGCAGCTTCC TTACTACAGA AGACAGCCAA AACCACCCCT 
TCTGCAGCAA AACCAGGCTC TACTCAGGGC AAATCAGCAC TCTCCAGCGC TGCTTTAGAC 
AAACCTGCCC ACCAGTGGAA GGATTCAGAC CCTGTCATCG CAGGAATCAG AGAGGAGATT 
GCACATTTTC AAAAGGAAAT GGAGGAGCTG AAGGCCCGGA CTTCCAAAGC CTGTTTCCAA 
GTGGGTACTC CAGAGGAAAT GAAAACGTTG CGAACTGAGT CAGATGATCT CCACACCTTC 
CTCTTGGAAA TTAAAGAAAC AACCGAGTCG CTGCATGGAG ATATTAGTAC TCTGAAGACA 
ACCTTGCTGG AAGGATTTGC AGGGGTAGAA GAGGCTAGAG AGCAGAATGA GAGGAATCAT 
AGCTTGGGGT ATTTGCACTT ACTTTATAAG AAACCTTTGG ATCCCAAGAG TGAAGCACAA 
CTTCAGGAGA TTCGACGCCT GCACCAGTAT GTAAAGTTTG CTGTTCAGGA TGTGAATGAT 
GTTCTGGATT TGGAATGGGA TCGGCACCTG GAACAGGAGA GAAAGCAGAA GCGTCTGATT 
GTTCCTGAAC GAGAGACTTT GTTTAACACC CTTGCTAATA ACCGGGAAAT AATCAATCAG 
CAGAAGAAGA GACTAAATCA GTTGGTGAAC AGCCTACAGC AGCTCCGACT CTATAATCAA 
ACATCTCAGT GGAGTGTTCC CAGTGATACA TCCTCTCCTA GCAGTACTCA AAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAGTGCCTTG ATGAAAACTA CCTTGGAATC ACGTGCCAAA 
CCCCCACCTA AAGCACCTGC TAAGTTGTCC CCTGTGAAGC TGACACAGTT GAGGAATTTT 
CTAGCCAAGA GGAAGACTCC ACCAGTTAGG TCTATGGCTC CAGCAAGTTT GTCCCGATCT 
GCCTTCTTGT CCACAAGATA CTATGAAGAC TTGGATGAAG TCAGTTCAAC ATCCTCAGTT 
TCTCAGTCTC TGGAGAATGA GGATCTGCAG GCAGCGGACC AGGAAGAGGA GGCTGCTCCC 
ATCCAAGTCC CTCGTCATGC CCCTGTGGTC CGCACTCCGT CTATCCAGCC TGGTTTGGTG 
GCTCAGCCTC CATCCTTTGG GAAACAACAC CCCTCCTTGG GCACTCTATT GCCTACATCT 
TCGAACAGAA TCATCCCACA AGGGGCAGAC AGCACAATGC TTGCAACAAA GACTGTGAAA 
CATGGGGCCC CTGCTCCCAC CGTCACTGTT CCAGCACCCC AGGCAGCTGC TGCGGCAGCC 
CTGCGGAGGC AGATGGCCAC CCAGTCCCCA GCTGTGAGCA CCTCCTTGAC TGAATCAACT 
CTGAAGAACG TTCCCCAGGT GGTGAATGTG CGAGAACTCA AGACTGGTGC GTTGACTCCA 
GCTGTCTCTA CAGTAATAGG GTCATCAGTT CCTCACTCTG CAGCCCAGGC AGTCCACCAA 
GTTCTGGCAA GTGTTGCTAC AAATCAGGCT AAGCAGACAC CTCAAACAAG CTCACTTAAG 
GGACAGGCTC CACCTTCTTC TGTATCTGCA TCTTGTCCTG TGGCATCTAC ACCTGGTCCC 
ACATCTGGCA ACAAAATCTC AGGACAAGGG ACAGCAAAAA CTGTTACAGC TGTGACCTCT 
ACAGTGGCGT CAGTACCAGC AACATCTGCA CAGCCTAACA AGGCTTTCTC ATTTTCTTCT 
GTGGGGTCTG GGTTTAATTT TGGTGTTGTC ACGTCGGCTG CTTCATCACC GACACTGCCC 
AGCCTTGCTG CCTCACAAGC CCCTGCTAAG GAGTCCACTC AACCTGATTC ATTTTCGCTT 
GCTGGGGGTT CCAAATTTGG AGCAGTCCCT GAGAGCTCCT TTGCTTTTGG CTCCCTCAAG 
CCAGCAAGTG CTTCTGGAGC CGCAGTAGCA AATAGCTCTT CAGCAGATGG CACTCAGTCA 
AACAAACCTG CTGCAACAAT GTCTTCTGCT CCATCCACAG CCTCTGGCAA ATTAGATATC 
CCTCAGTCTA AGCCAGGGGA CACCTTGTTT CTGAGCAGTC TGTCCGGGGA AACTCTCGGG 
AGCTTCTCAG GACTGCGTGT TGGTCAAGCA GATGATAATG CCAAAACCAC CACTAAAACA 
CCATCTACCA GTGCTGCAGG AGCACAGTCA ACTAAAGCAT CTACATCACC TTCTGGGTTT 
GTCTTTGGTA GCCCCCTGAT GTTAGGAAAA GCTGGAGAAG CTACTTCAAC ATCAGTCACG 
TCAGCTGGCA CGGCACCCAC CTCCATCAGC ACCAGTGCCA CAGGCAGTCT CTTTGGCAAT 
GTTCAGATAA CCAACACAGG CTCTCCTGGA GTATTTAATC TCGCAGGCTC TAGTGGAAAC 
AAACTCAACT TTTCTTTTGG TATTCAGCAA GCAAGCAGCA TGAGCACGTC CACTTCTGCC 
CCTTCTGCTC TCCCTGCTTC AACATCTCTC TCGTTCGGGA ATCTCTTGAG TCCGTCACAA 
ACTGCTGCCC CGGCTGCAAG TTCTATTAAA GGTGGAGGAG ATGCCAAACT GCTGTCTGCT 
TCAGAAAAGA CAGCTGAAAA TGAAGCACCT GAGTCTTCAC CTCCAACAAC GCAGACTCCA 
CAGCCACAGA TACAGCTTTC AGAGCCCGTT AAAGAGCCCC CTGCATCCCA GACAGCAGCT 
GGGGACACCA GCACCGTGGG CTCCAGTACT GCATCAGTAG CACCTCCCCC TGATGCCATT 
GCTACTCCTG CTTCCACAAG TGAGCTGAAG GCACCAGTGC CTCCTGCTGC CTCAGCCTCT 
GTCCCACAGG ATCAGAGCAT CTCAGAGGCG GCCTCTGCAA CAACCATTGC CTCTCCAGCA 
GAAGCAGCGA GCACACTGAC TGCTGCCGCT GGTGTTCCTA CATGTGTTCA GAGTCCAGCT 
GTTGGTGCCT CGGTGTTTGT CGCCACCTCT GCAGGCTCCT CAGTGTTCTC TCAGCCTCCA 
AATTCCAGCT CTTCTGGCTC AGCATTTAGC CAGCCTGCAG GTGATACAAC TGCCACTCCT 
TCCACACCGT CTGTTTTTGG CCAACTGCCT GCAGGTACCA CAGCATCAGC TCCATTTGGA 
CAACCCACCA CTGCCACCGT GACCACACCG ACTGCCAGCA CAGGGTTTGG TACTTCTGCC 
TTTGGCACCA CCACGACTGG GGGCTTTGGT CAGCCAGCCT TTGGCCAAGC ACCGACCTTT 
GGGCAGCCAG CAAGCAGTTC TTCAAGTGGT TTTACCTTCA ACCAGTCTGG ATTTGGGACA 
GTGCCTGCCT TTGGCCAGCC AGCAGCTTCC ACTGCAGCCT CAAGCAGCGG CAATGTATTT 
GGAGCATCTT CTAGCACCAA CAGTGCAAGC TCCTTCTCCT TCGGACAGCC ATCTGCCAGC 
ACTGGGGGCG GGATGTTTGG TCAAAGCAAC ACTCTCGTGT TTGGGCAAAG CAGCAGCTTT 
GGGCAAGGGG GATCTGTCTT TGGTAACACC GCCGCTGTGA CTACCACTAC ATCATCTGCT 
GGGTTCAGCT TCTGCCAGGC CTCAGGTTTT GGTTCTAGCA ACACAGGTTC TGTGTTTGGG 
CAAGCAGCAA ATACTGGAGG CATTGTCTTT GGGCAGCAGT CGTCATCTTC CACTGGAGGC 
CTCTTTGGAT CTGGAAGTGG TGGGAGAGGT GGAGGCTTCT TCACTGGTTT GGGAGGAAAG 
CCAAGTCAGG ATGCAGCCAA TAAGAATCCT TTTGGTTCCT CCAGTGGAGG ATTTGGATCG 
GCAGCTTCCC AGAATTCTTC TAGCTTATTT GGGAACAGTG GAGCCAAGGC CTTTGGATTT 
GGCAACTCAT CTTTTGGAGA CCAGAAACCC ACAGGCACTT TCAGCTCTGG TGGTGGCAGT 
GTGGCATCTC AAGGCTTTGG GTTCTCTAGT CCAACCAAAG CAGGTGGCTT CGGTGCTGCT 
CCAGTGTTTG GCAGCCCTCC CACTTTTGGG GGATCCCCTG GGTTTGGAGG GGTGCCAGCA 
TTCGGTTCAG CCCCAACCTT TTCAAGCCCT CTGGGCTCGA CGGGAGGCAA AGTGTTCGGC 
GAGGGTACAG CTGCAGCCAG CGCCGGAGGA TTTGGGTTTG GTGGTACCAG TAACAACACA 
GCATCATTTA GCACGCTAGC AAATCAGAAT ACCCCGACGT TTGGATCGCT GTCACAGCAG 
GGTACTGGTT TTGGCACACA GAACAGTGGA TTCTCTGCTT TCGGGACAGT TGGAGGAGGG 
TTTGGTTTTG GATCAACTAA CACATCTTCC TCTGGATTTA GCGGATGGAG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NUP214 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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