TCOF1 gene cDNA ORF clone, Ornithorhynchus anatinus(platypus)

mRNA	Protein	Name
XM_029050341.1	XP_028906174.1	treacle protein isoform X1
XM_029050342.1	XP_028906175.1	treacle protein isoform X2
XM_029050345.1	XP_028906178.1	treacle protein isoform X5
XM_029050346.1	XP_028906179.1	treacle protein isoform X6
XM_029050343.1	XP_028906176.1	treacle protein isoform X3
XM_029050347.1	XP_028906180.1	treacle protein isoform X7
XM_016228646.1	XP_016084132.1	treacle protein
XM_029050344.1	XP_028906177.1	treacle protein isoform X4
XM_029050348.1	XP_028906181.1	treacle protein isoform X8
XM_029050349.1	XP_028906182.1	treacle protein isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc135252	XM_029050341.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135253	XM_029050342.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135254	XM_029050343.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135255	XM_029050344.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135256	XM_029050345.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135257	XM_029050346.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135258	XM_029050347.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOc135259	XM_029050348.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc135260	XM_029050349.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOc38236	XM_016228646.1 Latest version!	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

TCOF1 ( XM_029050341.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906174.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc135252
Clone ID Related Accession (Same CDS sequence)	XM_029050341.1
Accession Version	XM_029050341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTCG GCGTTACCCC GGGATCTACC AAAAAGACCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA GGGCAGTGGT TACCACACTC CCCCAGATCA AGGTGAATAA AACCCCTCTG 
ATTTTTGTCG ACCCCAAACG TAGTCTGGCC GGCAAAGCAG CTGCCCCCAC CAAAGCCGCC 
GTGCCTGTCA GCGCCTCAAA GGAGGGGCGG GTCGCCGAAA GTGAGGGCAG CAGCTCATCC 
GGCAGCGAGG AAGAGGAAGA GGACCTGATT CCCGCCACGC AGAATCTGGA TACTTCCACG 
GCAAAACCGA CCGTGGTGGC TCTGCCCATC GCTCGCCGAG GGCCGGTCCC CGCTCCCAGG 
GCCAGCTCGC CTGAGGACAG CAGCGAGGAG TCTGACTCCG AAGAGGAAAA AAGCACACAG 
GTGAAGCCCA GCCCGGCTCT GGCCCCGTTG GCCGTGGCAA CCGTGAAGAC CTCAGGTCAG 
AAAGCCAGGA CGCCACAGCC ATCCGTGAAG GGGAGAAAGA CCTCGGGGTC GGCCCTCGCC 
CAACTTCCGG CGGCCTTGAA GATTCCCGCT CTGACGAAGG CAGCGCAGGA TGCTGGGGGA 
TTCCCTGGCT CAACCCCGAG TAGCATCCTC AAAGGAAGCA ATGGCAAGAA AGCCAAAGGC 
AAAGCAGGCC AGCCCCAGGC CTCCCCAACT AAGGCTGCTG GGGCCACCCG GAGAGCAGCC 
CACATCAACA GCAATAATTT GCCGGGGAAA AGACAGAAGG AGAATGGGGA GGGCGTGACC 
AAGGCCAAGT CAGCTCCCAA GAAGGGTGCA GCGCCAAAGG AGGAGGCCGG GATGGTGCCA 
GAGAGCTCTG AGGTGTCCAG CGAGGAGGAA GTGGAGCCTT CGCAGTCCCT GCTGTCAGGC 
TCTCCGGCTC CCCGACTCCC AGGGTCGACT CCCAAGTCCA CTCCGACCGT GCCTGCCAAG 
GCCCAGCCCC CAGAGACGCT TCCCCGGGGC CCTCCCAAGC CCGAAGTCGG GAAGACCACC 
GCCGCCAACG ACCCTCTGGG AGTGATAGTC CCTGCCAGCT CCGCCACCGA CGACAGCAGC 
GACGGAGGCA GCTCCGAGGA CGACGGCAAG CCGACGGCGG TCAAGCCGCC CGTAGGAACA 
GGAAGGGAGA CCTCTTGTGA AAAGGAGACG CCCGTCGCCC CCAGGCACCC GGTCACAGTC 
CCCAACTCGG CCCCACCCGT CCCCACCTCC AACCACAAGA GCACCCCGGG GACTAACGCT 
GCCTACGCTC TGCTGTCCCT GGCGAGGGCC GGGGCCGAGA GCTCCGGGGA CGGGGAACTG 
GACAGCGAGG AAGAGGCCTT GGCCACGTCC AGGGCCCAGG CCTCGGTGGT GAAGGTGCTG 
ACGGATCTGC TGCAGCAGGA GAAGAAGAAA GCCACTAAGA TCATCAAGGC TGCCAAGTCA 
CCCAAGGTCA AGGAGGGCAA GAAGCAGAAA TCCTCCCAGA AACGGAAACT GGCAGGGGAG 
GACGGGGACA CCAAGGCAAC CGGGAACAAG AAACAGAAGC TGGCAACGGA GGACTCTCAG 
GAGGGAGAGG CCAAGGCAGG CAAAGGCGGC AGGACTAAGA AAGGGAAAAA AGAAAAGGGG 
ACGGGGGATG CCCAGGAAAA GAGGGTCAGA GGGGCATCGG GCACCAAGAA ATCCAAAGAG 
AAGTCGGGCG ACGACCTGGA CCCCACAAAG GTGAGGAGCG AGGATGCCGG AGACACAAAG 
ACCAAGAAAG AAAAAAAGGA GAAGAAGAAA TCTGATAAGA AAGACAAGAC CCCCAAGGAC 
AAGAAGAAAC GGAAAAAGTC GTTGTCCAAA GACTCGGATT CCTCGGTGCT GAAGAAAAAA 
AAGAAAAAGG AAAAGAAAGT CGACCAGTCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906174.1
CDS	40..5715
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050341.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050341.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTCG GCGTTACCCC GGGATCTACC AAAAAGACCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA GGGCAGTGGT TACCACACTC CCCCAGATCA AGGTGAATAA AACCCCTCTG 
ATTTTTGTCG ACCCCAAACG TAGTCTGGCC GGCAAAGCAG CTGCCCCCAC CAAAGCCGCC 
GTGCCTGTCA GCGCCTCAAA GGAGGGGCGG GTCGCCGAAA GTGAGGGCAG CAGCTCATCC 
GGCAGCGAGG AAGAGGAAGA GGACCTGATT CCCGCCACGC AGAATCTGGA TACTTCCACG 
GCAAAACCGA CCGTGGTGGC TCTGCCCATC GCTCGCCGAG GGCCGGTCCC CGCTCCCAGG 
GCCAGCTCGC CTGAGGACAG CAGCGAGGAG TCTGACTCCG AAGAGGAAAA AAGCACACAG 
GTGAAGCCCA GCCCGGCTCT GGCCCCGTTG GCCGTGGCAA CCGTGAAGAC CTCAGGTCAG 
AAAGCCAGGA CGCCACAGCC ATCCGTGAAG GGGAGAAAGA CCTCGGGGTC GGCCCTCGCC 
CAACTTCCGG CGGCCTTGAA GATTCCCGCT CTGACGAAGG CAGCGCAGGA TGCTGGGGGA 
TTCCCTGGCT CAACCCCGAG TAGCATCCTC AAAGGAAGCA ATGGCAAGAA AGCCAAAGGC 
AAAGCAGGCC AGCCCCAGGC CTCCCCAACT AAGGCTGCTG GGGCCACCCG GAGAGCAGCC 
CACATCAACA GCAATAATTT GCCGGGGAAA AGACAGAAGG AGAATGGGGA GGGCGTGACC 
AAGGCCAAGT CAGCTCCCAA GAAGGGTGCA GCGCCAAAGG AGGAGGCCGG GATGGTGCCA 
GAGAGCTCTG AGGTGTCCAG CGAGGAGGAA GTGGAGCCTT CGCAGTCCCT GCTGTCAGGC 
TCTCCGGCTC CCCGACTCCC AGGGTCGACT CCCAAGTCCA CTCCGACCGT GCCTGCCAAG 
GCCCAGCCCC CAGAGACGCT TCCCCGGGGC CCTCCCAAGC CCGAAGTCGG GAAGACCACC 
GCCGCCAACG ACCCTCTGGG AGTGATAGTC CCTGCCAGCT CCGCCACCGA CGACAGCAGC 
GACGGAGGCA GCTCCGAGGA CGACGGCAAG CCGACGGCGG TCAAGCCGCC CGTAGGAACA 
GGAAGGGAGA CCTCTTGTGA AAAGGAGACG CCCGTCGCCC CCAGGCACCC GGTCACAGTC 
CCCAACTCGG CCCCACCCGT CCCCACCTCC AACCACAAGA GCACCCCGGG GACTAACGCT 
GCCTACGCTC TGCTGTCCCT GGCGAGGGCC GGGGCCGAGA GCTCCGGGGA CGGGGAACTG 
GACAGCGAGG AAGAGGCCTT GGCCACGTCC AGGGCCCAGG CCTCGGTGGT GAAGGTGCTG 
ACGGATCTGC TGCAGCAGGA GAAGAAGAAA GCCACTAAGA TCATCAAGGC TGCCAAGTCA 
CCCAAGGTCA AGGAGGGCAA GAAGCAGAAA TCCTCCCAGA AACGGAAACT GGCAGGGGAG 
GACGGGGACA CCAAGGCAAC CGGGAACAAG AAACAGAAGC TGGCAACGGA GGACTCTCAG 
GAGGGAGAGG CCAAGGCAGG CAAAGGCGGC AGGACTAAGA AAGGGAAAAA AGAAAAGGGG 
ACGGGGGATG CCCAGGAAAA GAGGGTCAGA GGGGCATCGG GCACCAAGAA ATCCAAAGAG 
AAGTCGGGCG ACGACCTGGA CCCCACAAAG GTGAGGAGCG AGGATGCCGG AGACACAAAG 
ACCAAGAAAG AAAAAAAGGA GAAGAAGAAA TCTGATAAGA AAGACAAGAC CCCCAAGGAC 
AAGAAGAAAC GGAAAAAGTC GTTGTCCAAA GACTCGGATT CCTCGGTGCT GAAGAAAAAA 
AAGAAAAAGG AAAAGAAAGT CGACCAGTCT AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050342.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906175.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

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CloneID	OOc135253
Clone ID Related Accession (Same CDS sequence)	XM_029050342.1
Accession Version	XM_029050342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGCGG GGCCGGTGGC TCGGAAACCA GAGAGCAGCA GCAGCGGGGC CTCCGGTGAC 
GAAACTGATA TTGAGACCCA GGTGAAACCA GGAGTAAAAG CTCAGCTCAC CAACACCCCA 
GGGAGAATGG TGCCAGCATC CACAGGACCA AAGGGAGGAA CATCTGCTCC AGGCACTGGG 
AAGCCGGTGA CCGCATCCCC CCAGGCAAAG GCGGGGACCC CAGTCAGGAT CGCTGGGGAC 
GGGAGGCCGG AGGAGAGCTC CGAGAGCAGC GAGGAGTCAG AGAGCGAGGA AGAGATGCCA 
GCCTCCCAGG GACAGGTGAA GCCAGCGGTC ACAGCACCCC ACGTCACCCC GGGGTCCACC 
AAAAAGCCCC AGGGCAGAGC GTCCACCCCA GCTGCCAGGA AGGCAGTGGC CGCCACGACT 
CCCAGGGCCC AGATCGGGGC TCCCGGCAAG GCCGTCGAGA TGAGGAGGGC AGAGGAGAGT 
TCCGAGAGCA GCGAGGAGTC CGAGGGTGAA GATGAGGTGC CGGCATCCCA GGGACAGGTG 
AAGGCAGCGG GGAGGACTCC TCTGGTCAGT TCTGTTCCGG TCAGCCCCGT CTCGGTCAAG 
GGGTCCCAGG AGAAAGCGAC CGCTCCAGCT CTCCGGAAGC CAGTGACCCA GCCCTCCCAG 
GCCAAGGTGG GGACGCCCAA CGTGGCCACC AAGGCAGGGG TGGAGAGTTC TGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAGGCACCG GCCCCCGAGG GAAAGGTTTC CCAGGCCAGG 
GCCCCCGCCG GGAGAGGTGC GGTCACGGCC CCAGCGTCCA CCAAGGGGGC GGCGGCCACA 
CACCCCCAGG CCCAGGCTAA GAAAGTGGGC AGCAGGAAGC CAGAGGAGAG CTCGGAGAGC 
AGCGAGTCAT CGGAGAGCGA GGAGGCATCG GCATCCAAGG GATCGGTCAA GGCAGCGGGG 
AGAACTCCCC GAGCCCTATC CGTGCCGGTC ACCCCCATCT CATTCACGGG GACCCATGAG 
AGAGTGTCCG CTTCGGTGGC CACACGACCG CAGGCCAAGG CGGGGACTCC CATCCAGGCC 
TCCGGGGCTG GGAGAGCAGA GGAGAGTTCA GAGAGCAGCG AGGAGTCGGA GAGCGAAGAG 
GAGGCACCGG CAGTCCAGGG ACCGGTGAAG CCAGCGGTCA CCGCATCCCA GGTCAGCGTC 
CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC AGAGCGTCCA CTCCAGCTGC CGGGAAGGCG 
ATGGCTGCCA CATCCCTCCA GGCTAAAATA GTGACTCCCA CCGCCAAGGC GGTTGGGGCC 
GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC GAGGAATCGG AGAGTGAAGA GGAGACTCCG 
GCGTCCCAGG GGCAGGTGAA GGCAGCGGGG AGAACTCCCC GGGCCGTCTC CGTTCCGGTC 
ACCCCCATCT CAGTCACGGG GACCCATGAG AGAGTTTCCA CTTCGGTGGC CACGCAGCTG 
CGGACCAAGG CGGGGACTCC CGTCCAGGCT GAGAAGGCGG AGAAGAGTTC AGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAAGCACCG GCAGTCAGAG GAAAGGGGAA GCCAGTGGTC 
ATCGCATCCC AGGTCGGCAT CACTCCGGGA TCTGCCAGAA AGCCCCAGGG CAGAGTGTCC 
ACTCCAGCTG CCGGGAACCG GGTGGCTGCC ACAGCCCCCA TGGCTAAGGT AGCGACTCCT 
ACCACCAAGG CCTCTGGGGG CGGGAAACCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCG 
GAGAGCGAAG AAGAGGCGCC GGCGTCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACTCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAATCAGCG GGACCCATGA GAGAGCGTCC 
GCTTCGGTGG CCACACGACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGGAGAGCAG AGAACAGTTC AGAGAGCAGC GAGGAGTCGG AGAGCGAAGA GGAGGCACCG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCAGCGT CACTCGGGGA 
TCTGCCAAAA AGCCCCAGGG CAGAGCGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACATCCCTCC AGGCTAAGGT AGTGACCCCC ACCGCCAAGG CGGTTGGGGC CGGGAAGCCA 
GGAGAGAGTT CTGAGAGCAG CGAGGAGTCG GAGAGTGAAG AGGAGGCTCC GGCGTCCCAG 
GGGCAGGTGG AGGCAGCGGG GAGAACTCCC CGAGCCGTCT CCGTTCCGGT CACCCCCATC 
TCAGTCACGG GGACCCATGA GAGAGTGTCC ACGTCGGTGG CCACGCAGCC GCAGGCCAAG 
GCGGGGACTC CCATCAAGGC CTCCGGGGCT GGGAGAGCAG AGAAGAGTTC AGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAGGCACCG ACAGTCAGAG GACAGGTGAA GCCAACGGTC 
ACCGCATCTC AGGTCAGTGT CACTCCGGGG TCCATCAAGA AGTTCCAAGG CAAAGCATCC 
ACTCCAGCTG CTGGGAAGGG GGTGGCTACC AAGTCCACCC GGGCCAAGGT CGTGACTTCT 
ACCGATGCCT CGGGGGGCGG GAAGCCAGGG GAGAGTTCTG AGAGCAGCGA GGAGTCAGAG 
AGCGAAGAGG AGACTCCGGC ATCCCAGGGG CAGGTGAAGG CAGCGGGGAG AACGCCCCGG 
GCCGTCTCCG TTCCGGTCAC CCCCATCTCA GTCACGGGGA CCTATGAGAG AGTGTCCGCT 
TCGGTGACCA CACAACCGCA GGCCAAGGCG GGGACTCCCA TCAAGGCCTC CGGGGCTGGA 
AGAGCAGAGA AGAGTTCAGA GAGCAGCGAC GAGTCGGAGA GCGAAGAGGA GGCACTGGCA 
GTCCAGGGAC CGGTGAAGCC AGCGGTCACC ACATCCCAGG TCGGCGTCCC TCTGGGATCT 
GCCAAAAAGC CTCAGGGCAG AGGGTCCACT CCAGCTGCCG GGAAGGCGAT GGCTGCCACA 
CCCCTCCAGG CTAAGGTAGT GACTCCCACC GCCAAGGCGG CTGGGGGCGG GAAGCCAGGG 
GAGAGTTCCG AGAGCAGCGA GGAATCGGAG AGTGAAGAGG AGACTCCGGC GTCCCAGGGG 
CAGGTCGGCG TTACCCCGGG ATCTACCAAA AAGACCCAGG GCAGAGCGTC CACTCCAGCT 
GCCGGGAGGG CAGTGGTTAC CACACTCCCC CAGATCAAGG TGAATAAAAC CCCTCTGATT 
TTTGTCGACC CCAAACGTAG TCTGGCCGGC AAAGCAGCTG CCCCCACCAA AGCCGCCGTG 
CCTGTCAGCG CCTCAAAGGA GGGGCGGGTC GCCGAAAGTG AGGGCAGCAG CTCATCCGGC 
AGCGAGGAAG AGGAAGAGGA CCTGATTCCC GCCACGCAGA ATCTGGATAC TTCCACGGCA 
AAACCGACCG TGGTGGCTCT GCCCATCGCT CGCCGAGGGC CGGTCCCCGC TCCCAGGGCC 
AGCTCGCCTG AGGACAGCAG CGAGGAGTCT GACTCCGAAG AGGAAAAAAG CACACAGGTG 
AAGCCCAGCC CGGCTCTGGC CCCGTTGGCC GTGGCAACCG TGAAGACCTC AGGTCAGAAA 
GCCAGGACGC CACAGCCATC CGTGAAGGGG AGAAAGACCT CGGGGTCGGC CCTCGCCCAA 
CTTCCGGCGG CCTTGAAGAT TCCCGCTCTG ACGAAGGCAG CGCAGGATGC TGGGGGATTC 
CCTGGCTCAA CCCCGAGTAG CATCCTCAAA GGAAGCAATG GCAAGAAAGC CAAAGGCAAA 
GCAGGCCAGC CCCAGGCCTC CCCAACTAAG GCTGCTGGGG CCACCCGGAG AGCAGCCCAC 
ATCAACAGCA ATAATTTGCC GGGGAAAAGA CAGAAGGAGA ATGGGGAGGG CGTGACCAAG 
GCCAAGTCAG CTCCCAAGAA GGGTGCAGCG CCAAAGGAGG AGGCCGGGAT GGTGCCAGAG 
AGCTCTGAGG TGTCCAGCGA GGAGGAAGTG GAGCCTTCGC AGTCCCTGCT GTCAGGCTCT 
CCGGCTCCCC GACTCCCAGG GTCGACTCCC AAGTCCACTC CGACCGTGCC TGCCAAGGCC 
CAGCCCCCAG AGACGCTTCC CCGGGGCCCT CCCAAGCCCG AAGTCGGGAA GACCACCGCC 
GCCAACGACC CTCTGGGAGT GATAGTCCCT GCCAGCTCCG CCACCGACGA CAGCAGCGAC 
GGAGGCAGCT CCGAGGACGA CGGCAAGCCG ACGGCGGTCA AGCCGCCCGT AGGAACAGGA 
AGGGAGACCT CTTGTGAAAA GGAGACGCCC GTCGCCCCCA GGCACCCGGT CACAGTCCCC 
AACTCGGCCC CACCCGTCCC CACCTCCAAC CACAAGAGCA CCCCGGGGAC TAACGCTGCC 
TACGCTCTGC TGTCCCTGGC GAGGGCCGGG GCCGAGAGCT CCGGGGACGG GGAACTGGAC 
AGCGAGGAAG AGGCCTTGGC CACGTCCAGG GCCCAGGCCT CGGTGGTGAA GGTGCTGACG 
GATCTGCTGC AGCAGGAGAA GAAGAAAGCC ACTAAGATCA TCAAGGCTGC CAAGTCACCC 
AAGGTCAAGG AGGGCAAGAA GCAGAAATCC TCCCAGAAAC GGAAACTGGC AGGGGAGGAC 
GGGGACACCA AGGCAACCGG GAACAAGAAA CAGAAGCTGG CAACGGAGGA CTCTCAGGAG 
GGAGAGGCCA AGGCAGGCAA AGGCGGCAGG ACTAAGAAAG GGAAAAAAGA AAAGGGGACG 
GGGGATGCCC AGGAAAAGAG GGTCAGAGGG GCATCGGGCA CCAAGAAATC CAAAGAGAAG 
TCGGGCGACG ACCTGGACCC CACAAAGGTG AGGAGCGAGG ATGCCGGAGA CACAAAGACC 
AAGAAAGAAA AAAAGGAGAA GAAGAAATCT GATAAGAAAG ACAAGACCCC CAAGGACAAG 
AAGAAACGGA AAAAGTCGTT GTCCAAAGAC TCGGATTCCT CGGTGCTGAA GAAAAAAAAG 
AAAAAGGAAA AGAAAGTCGA CCAGTCTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906175.1
CDS	41..5713
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050342.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050342.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGCGG GGCCGGTGGC TCGGAAACCA GAGAGCAGCA GCAGCGGGGC CTCCGGTGAC 
GAAACTGATA TTGAGACCCA GGTGAAACCA GGAGTAAAAG CTCAGCTCAC CAACACCCCA 
GGGAGAATGG TGCCAGCATC CACAGGACCA AAGGGAGGAA CATCTGCTCC AGGCACTGGG 
AAGCCGGTGA CCGCATCCCC CCAGGCAAAG GCGGGGACCC CAGTCAGGAT CGCTGGGGAC 
GGGAGGCCGG AGGAGAGCTC CGAGAGCAGC GAGGAGTCAG AGAGCGAGGA AGAGATGCCA 
GCCTCCCAGG GACAGGTGAA GCCAGCGGTC ACAGCACCCC ACGTCACCCC GGGGTCCACC 
AAAAAGCCCC AGGGCAGAGC GTCCACCCCA GCTGCCAGGA AGGCAGTGGC CGCCACGACT 
CCCAGGGCCC AGATCGGGGC TCCCGGCAAG GCCGTCGAGA TGAGGAGGGC AGAGGAGAGT 
TCCGAGAGCA GCGAGGAGTC CGAGGGTGAA GATGAGGTGC CGGCATCCCA GGGACAGGTG 
AAGGCAGCGG GGAGGACTCC TCTGGTCAGT TCTGTTCCGG TCAGCCCCGT CTCGGTCAAG 
GGGTCCCAGG AGAAAGCGAC CGCTCCAGCT CTCCGGAAGC CAGTGACCCA GCCCTCCCAG 
GCCAAGGTGG GGACGCCCAA CGTGGCCACC AAGGCAGGGG TGGAGAGTTC TGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAGGCACCG GCCCCCGAGG GAAAGGTTTC CCAGGCCAGG 
GCCCCCGCCG GGAGAGGTGC GGTCACGGCC CCAGCGTCCA CCAAGGGGGC GGCGGCCACA 
CACCCCCAGG CCCAGGCTAA GAAAGTGGGC AGCAGGAAGC CAGAGGAGAG CTCGGAGAGC 
AGCGAGTCAT CGGAGAGCGA GGAGGCATCG GCATCCAAGG GATCGGTCAA GGCAGCGGGG 
AGAACTCCCC GAGCCCTATC CGTGCCGGTC ACCCCCATCT CATTCACGGG GACCCATGAG 
AGAGTGTCCG CTTCGGTGGC CACACGACCG CAGGCCAAGG CGGGGACTCC CATCCAGGCC 
TCCGGGGCTG GGAGAGCAGA GGAGAGTTCA GAGAGCAGCG AGGAGTCGGA GAGCGAAGAG 
GAGGCACCGG CAGTCCAGGG ACCGGTGAAG CCAGCGGTCA CCGCATCCCA GGTCAGCGTC 
CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC AGAGCGTCCA CTCCAGCTGC CGGGAAGGCG 
ATGGCTGCCA CATCCCTCCA GGCTAAAATA GTGACTCCCA CCGCCAAGGC GGTTGGGGCC 
GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC GAGGAATCGG AGAGTGAAGA GGAGACTCCG 
GCGTCCCAGG GGCAGGTGAA GGCAGCGGGG AGAACTCCCC GGGCCGTCTC CGTTCCGGTC 
ACCCCCATCT CAGTCACGGG GACCCATGAG AGAGTTTCCA CTTCGGTGGC CACGCAGCTG 
CGGACCAAGG CGGGGACTCC CGTCCAGGCT GAGAAGGCGG AGAAGAGTTC AGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAAGCACCG GCAGTCAGAG GAAAGGGGAA GCCAGTGGTC 
ATCGCATCCC AGGTCGGCAT CACTCCGGGA TCTGCCAGAA AGCCCCAGGG CAGAGTGTCC 
ACTCCAGCTG CCGGGAACCG GGTGGCTGCC ACAGCCCCCA TGGCTAAGGT AGCGACTCCT 
ACCACCAAGG CCTCTGGGGG CGGGAAACCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCG 
GAGAGCGAAG AAGAGGCGCC GGCGTCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACTCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAATCAGCG GGACCCATGA GAGAGCGTCC 
GCTTCGGTGG CCACACGACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGGAGAGCAG AGAACAGTTC AGAGAGCAGC GAGGAGTCGG AGAGCGAAGA GGAGGCACCG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCAGCGT CACTCGGGGA 
TCTGCCAAAA AGCCCCAGGG CAGAGCGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACATCCCTCC AGGCTAAGGT AGTGACCCCC ACCGCCAAGG CGGTTGGGGC CGGGAAGCCA 
GGAGAGAGTT CTGAGAGCAG CGAGGAGTCG GAGAGTGAAG AGGAGGCTCC GGCGTCCCAG 
GGGCAGGTGG AGGCAGCGGG GAGAACTCCC CGAGCCGTCT CCGTTCCGGT CACCCCCATC 
TCAGTCACGG GGACCCATGA GAGAGTGTCC ACGTCGGTGG CCACGCAGCC GCAGGCCAAG 
GCGGGGACTC CCATCAAGGC CTCCGGGGCT GGGAGAGCAG AGAAGAGTTC AGAGAGCAGC 
GAGGAGTCGG AGAGTGAAGA GGAGGCACCG ACAGTCAGAG GACAGGTGAA GCCAACGGTC 
ACCGCATCTC AGGTCAGTGT CACTCCGGGG TCCATCAAGA AGTTCCAAGG CAAAGCATCC 
ACTCCAGCTG CTGGGAAGGG GGTGGCTACC AAGTCCACCC GGGCCAAGGT CGTGACTTCT 
ACCGATGCCT CGGGGGGCGG GAAGCCAGGG GAGAGTTCTG AGAGCAGCGA GGAGTCAGAG 
AGCGAAGAGG AGACTCCGGC ATCCCAGGGG CAGGTGAAGG CAGCGGGGAG AACGCCCCGG 
GCCGTCTCCG TTCCGGTCAC CCCCATCTCA GTCACGGGGA CCTATGAGAG AGTGTCCGCT 
TCGGTGACCA CACAACCGCA GGCCAAGGCG GGGACTCCCA TCAAGGCCTC CGGGGCTGGA 
AGAGCAGAGA AGAGTTCAGA GAGCAGCGAC GAGTCGGAGA GCGAAGAGGA GGCACTGGCA 
GTCCAGGGAC CGGTGAAGCC AGCGGTCACC ACATCCCAGG TCGGCGTCCC TCTGGGATCT 
GCCAAAAAGC CTCAGGGCAG AGGGTCCACT CCAGCTGCCG GGAAGGCGAT GGCTGCCACA 
CCCCTCCAGG CTAAGGTAGT GACTCCCACC GCCAAGGCGG CTGGGGGCGG GAAGCCAGGG 
GAGAGTTCCG AGAGCAGCGA GGAATCGGAG AGTGAAGAGG AGACTCCGGC GTCCCAGGGG 
CAGGTCGGCG TTACCCCGGG ATCTACCAAA AAGACCCAGG GCAGAGCGTC CACTCCAGCT 
GCCGGGAGGG CAGTGGTTAC CACACTCCCC CAGATCAAGG TGAATAAAAC CCCTCTGATT 
TTTGTCGACC CCAAACGTAG TCTGGCCGGC AAAGCAGCTG CCCCCACCAA AGCCGCCGTG 
CCTGTCAGCG CCTCAAAGGA GGGGCGGGTC GCCGAAAGTG AGGGCAGCAG CTCATCCGGC 
AGCGAGGAAG AGGAAGAGGA CCTGATTCCC GCCACGCAGA ATCTGGATAC TTCCACGGCA 
AAACCGACCG TGGTGGCTCT GCCCATCGCT CGCCGAGGGC CGGTCCCCGC TCCCAGGGCC 
AGCTCGCCTG AGGACAGCAG CGAGGAGTCT GACTCCGAAG AGGAAAAAAG CACACAGGTG 
AAGCCCAGCC CGGCTCTGGC CCCGTTGGCC GTGGCAACCG TGAAGACCTC AGGTCAGAAA 
GCCAGGACGC CACAGCCATC CGTGAAGGGG AGAAAGACCT CGGGGTCGGC CCTCGCCCAA 
CTTCCGGCGG CCTTGAAGAT TCCCGCTCTG ACGAAGGCAG CGCAGGATGC TGGGGGATTC 
CCTGGCTCAA CCCCGAGTAG CATCCTCAAA GGAAGCAATG GCAAGAAAGC CAAAGGCAAA 
GCAGGCCAGC CCCAGGCCTC CCCAACTAAG GCTGCTGGGG CCACCCGGAG AGCAGCCCAC 
ATCAACAGCA ATAATTTGCC GGGGAAAAGA CAGAAGGAGA ATGGGGAGGG CGTGACCAAG 
GCCAAGTCAG CTCCCAAGAA GGGTGCAGCG CCAAAGGAGG AGGCCGGGAT GGTGCCAGAG 
AGCTCTGAGG TGTCCAGCGA GGAGGAAGTG GAGCCTTCGC AGTCCCTGCT GTCAGGCTCT 
CCGGCTCCCC GACTCCCAGG GTCGACTCCC AAGTCCACTC CGACCGTGCC TGCCAAGGCC 
CAGCCCCCAG AGACGCTTCC CCGGGGCCCT CCCAAGCCCG AAGTCGGGAA GACCACCGCC 
GCCAACGACC CTCTGGGAGT GATAGTCCCT GCCAGCTCCG CCACCGACGA CAGCAGCGAC 
GGAGGCAGCT CCGAGGACGA CGGCAAGCCG ACGGCGGTCA AGCCGCCCGT AGGAACAGGA 
AGGGAGACCT CTTGTGAAAA GGAGACGCCC GTCGCCCCCA GGCACCCGGT CACAGTCCCC 
AACTCGGCCC CACCCGTCCC CACCTCCAAC CACAAGAGCA CCCCGGGGAC TAACGCTGCC 
TACGCTCTGC TGTCCCTGGC GAGGGCCGGG GCCGAGAGCT CCGGGGACGG GGAACTGGAC 
AGCGAGGAAG AGGCCTTGGC CACGTCCAGG GCCCAGGCCT CGGTGGTGAA GGTGCTGACG 
GATCTGCTGC AGCAGGAGAA GAAGAAAGCC ACTAAGATCA TCAAGGCTGC CAAGTCACCC 
AAGGTCAAGG AGGGCAAGAA GCAGAAATCC TCCCAGAAAC GGAAACTGGC AGGGGAGGAC 
GGGGACACCA AGGCAACCGG GAACAAGAAA CAGAAGCTGG CAACGGAGGA CTCTCAGGAG 
GGAGAGGCCA AGGCAGGCAA AGGCGGCAGG ACTAAGAAAG GGAAAAAAGA AAAGGGGACG 
GGGGATGCCC AGGAAAAGAG GGTCAGAGGG GCATCGGGCA CCAAGAAATC CAAAGAGAAG 
TCGGGCGACG ACCTGGACCC CACAAAGGTG AGGAGCGAGG ATGCCGGAGA CACAAAGACC 
AAGAAAGAAA AAAAGGAGAA GAAGAAATCT GATAAGAAAG ACAAGACCCC CAAGGACAAG 
AAGAAACGGA AAAAGTCGTT GTCCAAAGAC TCGGATTCCT CGGTGCTGAA GAAAAAAAAG 
AAAAAGGAAA AGAAAGTCGA CCAGTCTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050343.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906176.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOc135254
Clone ID Related Accession (Same CDS sequence)	XM_029050343.1
Accession Version	XM_029050343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTGA ATAAAACCCC TCTGATTTTT GTCGACCCCA AACGTAGTCT GGCCGGCAAA 
GCAGCTGCCC CCACCAAAGC CGCCGTGCCT GTCAGCGCCT CAAAGGAGGG GCGGGTCGCC 
GAAAGTGAGG GCAGCAGCTC ATCCGGCAGC GAGGAAGAGG AAGAGGACCT GATTCCCGCC 
ACGCAGAATC TGGATACTTC CACGGCAAAA CCGACCGTGG TGGCTCTGCC CATCGCTCGC 
CGAGGGCCGG TCCCCGCTCC CAGGGCCAGC TCGCCTGAGG ACAGCAGCGA GGAGTCTGAC 
TCCGAAGAGG AAAAAAGCAC ACAGGTGAAG CCCAGCCCGG CTCTGGCCCC GTTGGCCGTG 
GCAACCGTGA AGACCTCAGG TCAGAAAGCC AGGACGCCAC AGCCATCCGT GAAGGGGAGA 
AAGACCTCGG GGTCGGCCCT CGCCCAACTT CCGGCGGCCT TGAAGATTCC CGCTCTGACG 
AAGGCAGCGC AGGATGCTGG GGGATTCCCT GGCTCAACCC CGAGTAGCAT CCTCAAAGGA 
AGCAATGGCA AGAAAGCCAA AGGCAAAGCA GGCCAGCCCC AGGCCTCCCC AACTAAGGCT 
GCTGGGGCCA CCCGGAGAGC AGCCCACATC AACAGCAATA ATTTGCCGGG GAAAAGACAG 
AAGGAGAATG GGGAGGGCGT GACCAAGGCC AAGTCAGCTC CCAAGAAGGG TGCAGCGCCA 
AAGGAGGAGG CCGGGATGGT GCCAGAGAGC TCTGAGGTGT CCAGCGAGGA GGAAGTGGAG 
CCTTCGCAGT CCCTGCTGTC AGGCTCTCCG GCTCCCCGAC TCCCAGGGTC GACTCCCAAG 
TCCACTCCGA CCGTGCCTGC CAAGGCCCAG CCCCCAGAGA CGCTTCCCCG GGGCCCTCCC 
AAGCCCGAAG TCGGGAAGAC CACCGCCGCC AACGACCCTC TGGGAGTGAT AGTCCCTGCC 
AGCTCCGCCA CCGACGACAG CAGCGACGGA GGCAGCTCCG AGGACGACGG CAAGCCGACG 
GCGGTCAAGC CGCCCGTAGG AACAGGAAGG GAGACCTCTT GTGAAAAGGA GACGCCCGTC 
GCCCCCAGGC ACCCGGTCAC AGTCCCCAAC TCGGCCCCAC CCGTCCCCAC CTCCAACCAC 
AAGAGCACCC CGGGGACTAA CGCTGCCTAC GCTCTGCTGT CCCTGGCGAG GGCCGGGGCC 
GAGAGCTCCG GGGACGGGGA ACTGGACAGC GAGGAAGAGG CCTTGGCCAC GTCCAGGGCC 
CAGGCCTCGG TGGTGAAGGT GCTGACGGAT CTGCTGCAGC AGGAGAAGAA GAAAGCCACT 
AAGATCATCA AGGCTGCCAA GTCACCCAAG GTCAAGGAGG GCAAGAAGCA GAAATCCTCC 
CAGAAACGGA AACTGGCAGG GGAGGACGGG GACACCAAGG CAACCGGGAA CAAGAAACAG 
AAGCTGGCAA CGGAGGACTC TCAGGAGGGA GAGGCCAAGG CAGGCAAAGG CGGCAGGACT 
AAGAAAGGGA AAAAAGAAAA GGGGACGGGG GATGCCCAGG AAAAGAGGGT CAGAGGGGCA 
TCGGGCACCA AGAAATCCAA AGAGAAGTCG GGCGACGACC TGGACCCCAC AAAGGTGAGG 
AGCGAGGATG CCGGAGACAC AAAGACCAAG AAAGAAAAAA AGGAGAAGAA GAAATCTGAT 
AAGAAAGACA AGACCCCCAA GGACAAGAAG AAACGGAAAA AGTCGTTGTC CAAAGACTCG 
GATTCCTCGG TGCTGAAGAA AAAAAAGAAA AAGGAAAAGA AAGTCGACCA GTCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906176.1
CDS	40..5619
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050343.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050343.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTGA ATAAAACCCC TCTGATTTTT GTCGACCCCA AACGTAGTCT GGCCGGCAAA 
GCAGCTGCCC CCACCAAAGC CGCCGTGCCT GTCAGCGCCT CAAAGGAGGG GCGGGTCGCC 
GAAAGTGAGG GCAGCAGCTC ATCCGGCAGC GAGGAAGAGG AAGAGGACCT GATTCCCGCC 
ACGCAGAATC TGGATACTTC CACGGCAAAA CCGACCGTGG TGGCTCTGCC CATCGCTCGC 
CGAGGGCCGG TCCCCGCTCC CAGGGCCAGC TCGCCTGAGG ACAGCAGCGA GGAGTCTGAC 
TCCGAAGAGG AAAAAAGCAC ACAGGTGAAG CCCAGCCCGG CTCTGGCCCC GTTGGCCGTG 
GCAACCGTGA AGACCTCAGG TCAGAAAGCC AGGACGCCAC AGCCATCCGT GAAGGGGAGA 
AAGACCTCGG GGTCGGCCCT CGCCCAACTT CCGGCGGCCT TGAAGATTCC CGCTCTGACG 
AAGGCAGCGC AGGATGCTGG GGGATTCCCT GGCTCAACCC CGAGTAGCAT CCTCAAAGGA 
AGCAATGGCA AGAAAGCCAA AGGCAAAGCA GGCCAGCCCC AGGCCTCCCC AACTAAGGCT 
GCTGGGGCCA CCCGGAGAGC AGCCCACATC AACAGCAATA ATTTGCCGGG GAAAAGACAG 
AAGGAGAATG GGGAGGGCGT GACCAAGGCC AAGTCAGCTC CCAAGAAGGG TGCAGCGCCA 
AAGGAGGAGG CCGGGATGGT GCCAGAGAGC TCTGAGGTGT CCAGCGAGGA GGAAGTGGAG 
CCTTCGCAGT CCCTGCTGTC AGGCTCTCCG GCTCCCCGAC TCCCAGGGTC GACTCCCAAG 
TCCACTCCGA CCGTGCCTGC CAAGGCCCAG CCCCCAGAGA CGCTTCCCCG GGGCCCTCCC 
AAGCCCGAAG TCGGGAAGAC CACCGCCGCC AACGACCCTC TGGGAGTGAT AGTCCCTGCC 
AGCTCCGCCA CCGACGACAG CAGCGACGGA GGCAGCTCCG AGGACGACGG CAAGCCGACG 
GCGGTCAAGC CGCCCGTAGG AACAGGAAGG GAGACCTCTT GTGAAAAGGA GACGCCCGTC 
GCCCCCAGGC ACCCGGTCAC AGTCCCCAAC TCGGCCCCAC CCGTCCCCAC CTCCAACCAC 
AAGAGCACCC CGGGGACTAA CGCTGCCTAC GCTCTGCTGT CCCTGGCGAG GGCCGGGGCC 
GAGAGCTCCG GGGACGGGGA ACTGGACAGC GAGGAAGAGG CCTTGGCCAC GTCCAGGGCC 
CAGGCCTCGG TGGTGAAGGT GCTGACGGAT CTGCTGCAGC AGGAGAAGAA GAAAGCCACT 
AAGATCATCA AGGCTGCCAA GTCACCCAAG GTCAAGGAGG GCAAGAAGCA GAAATCCTCC 
CAGAAACGGA AACTGGCAGG GGAGGACGGG GACACCAAGG CAACCGGGAA CAAGAAACAG 
AAGCTGGCAA CGGAGGACTC TCAGGAGGGA GAGGCCAAGG CAGGCAAAGG CGGCAGGACT 
AAGAAAGGGA AAAAAGAAAA GGGGACGGGG GATGCCCAGG AAAAGAGGGT CAGAGGGGCA 
TCGGGCACCA AGAAATCCAA AGAGAAGTCG GGCGACGACC TGGACCCCAC AAAGGTGAGG 
AGCGAGGATG CCGGAGACAC AAAGACCAAG AAAGAAAAAA AGGAGAAGAA GAAATCTGAT 
AAGAAAGACA AGACCCCCAA GGACAAGAAG AAACGGAAAA AGTCGTTGTC CAAAGACTCG 
GATTCCTCGG TGCTGAAGAA AAAAAAGAAA AAGGAAAAGA AAGTCGACCA GTCTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050344.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906177.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

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CloneID	OOc135255
Clone ID Related Accession (Same CDS sequence)	XM_029050344.1
Accession Version	XM_029050344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTGAAGGCA 
GCGGGGAGAA CTCCCCGGGC CGTCTCCGTT CCGGTCACCC CCATCTCAAT CAGCGGGACC 
CATGAGAGAG CGTCCGCTTC GGTGGCCACA CGACCGCAGG CCAAGGCGGG GACTCCCATC 
AAGGCCTCCG GGGCTGGGAG AGCAGAGAAC AGTTCAGAGA GCAGCGAGGA GTCGGAGAGC 
GAAGAGGAGG CACCGGCAGT CCAGGGACCG GTGAAGCCAG CGGTCACCAC ATCCCAGGTC 
AGCGTCACTC GGGGATCTGC CAAAAAGCCC CAGGGCAGAG CGTCCACTCC AGCTGCCGGG 
AAGGCGATGG CTGCCACATC CCTCCAGGCT AAGGTAGTGA CCCCCACCGC CAAGGCGGTT 
GGGGCCGGGA AGCCAGGAGA GAGTTCTGAG AGCAGCGAGG AGTCGGAGAG TGAAGAGGAG 
GCTCCGGCGT CCCAGGGGCA GGTGGAGGCA GCGGGGAGAA CTCCCCGAGC CGTCTCCGTT 
CCGGTCACCC CCATCTCAGT CACGGGGACC CATGAGAGAG TGTCCACGTC GGTGGCCACG 
CAGCCGCAGG CCAAGGCGGG GACTCCCATC AAGGCCTCCG GGGCTGGGAG AGCAGAGAAG 
AGTTCAGAGA GCAGCGAGGA GTCGGAGAGT GAAGAGGAGG CACCGACAGT CAGAGGACAG 
GTGAAGCCAA CGGTCACCGC ATCTCAGGTC AGTGTCACTC CGGGGTCCAT CAAGAAGTTC 
CAAGGCAAAG CATCCACTCC AGCTGCTGGG AAGGGGGTGG CTACCAAGTC CACCCGGGCC 
AAGGTCGTGA CTTCTACCGA TGCCTCGGGG GGCGGGAAGC CAGGGGAGAG TTCTGAGAGC 
AGCGAGGAGT CAGAGAGCGA AGAGGAGACT CCGGCATCCC AGGGGCAGGT GAAGGCAGCG 
GGGAGAACGC CCCGGGCCGT CTCCGTTCCG GTCACCCCCA TCTCAGTCAC GGGGACCTAT 
GAGAGAGTGT CCGCTTCGGT GACCACACAA CCGCAGGCCA AGGCGGGGAC TCCCATCAAG 
GCCTCCGGGG CTGGAAGAGC AGAGAAGAGT TCAGAGAGCA GCGACGAGTC GGAGAGCGAA 
GAGGAGGCAC TGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCACATC CCAGGTCGGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGGGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACACCCCT CCAGGCTAAG GTAGTGACTC CCACCGCCAA GGCGGCTGGG 
GGCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT CGGCGTTACC CCGGGATCTA CCAAAAAGAC CCAGGGCAGA 
GCGTCCACTC CAGCTGCCGG GAGGGCAGTG GTTACCACAC TCCCCCAGAT CAAGGTGAAT 
AAAACCCCTC TGATTTTTGT CGACCCCAAA CGTAGTCTGG CCGGCAAAGC AGCTGCCCCC 
ACCAAAGCCG CCGTGCCTGT CAGCGCCTCA AAGGAGGGGC GGGTCGCCGA AAGTGAGGGC 
AGCAGCTCAT CCGGCAGCGA GGAAGAGGAA GAGGACCTGA TTCCCGCCAC GCAGAATCTG 
GATACTTCCA CGGCAAAACC GACCGTGGTG GCTCTGCCCA TCGCTCGCCG AGGGCCGGTC 
CCCGCTCCCA GGGCCAGCTC GCCTGAGGAC AGCAGCGAGG AGTCTGACTC CGAAGAGGAA 
AAAAGCACAC AGGTGAAGCC CAGCCCGGCT CTGGCCCCGT TGGCCGTGGC AACCGTGAAG 
ACCTCAGGTC AGAAAGCCAG GACGCCACAG CCATCCGTGA AGGGGAGAAA GACCTCGGGG 
TCGGCCCTCG CCCAACTTCC GGCGGCCTTG AAGATTCCCG CTCTGACGAA GGCAGCGCAG 
GATGCTGGGG GATTCCCTGG CTCAACCCCG AGTAGCATCC TCAAAGGAAG CAATGGCAAG 
AAAGCCAAAG GCAAAGCAGG CCAGCCCCAG GCCTCCCCAA CTAAGGCTGC TGGGGCCACC 
CGGAGAGCAG CCCACATCAA CAGCAATAAT TTGCCGGGGA AAAGACAGAA GGAGAATGGG 
GAGGGCGTGA CCAAGGCCAA GTCAGCTCCC AAGAAGGGTG CAGCGCCAAA GGAGGAGGCC 
GGGATGGTGC CAGAGAGCTC TGAGGTGTCC AGCGAGGAGG AAGTGGAGCC TTCGCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCA CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAAAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906177.1
CDS	40..5607
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050344.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050344.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTGAAGGCA 
GCGGGGAGAA CTCCCCGGGC CGTCTCCGTT CCGGTCACCC CCATCTCAAT CAGCGGGACC 
CATGAGAGAG CGTCCGCTTC GGTGGCCACA CGACCGCAGG CCAAGGCGGG GACTCCCATC 
AAGGCCTCCG GGGCTGGGAG AGCAGAGAAC AGTTCAGAGA GCAGCGAGGA GTCGGAGAGC 
GAAGAGGAGG CACCGGCAGT CCAGGGACCG GTGAAGCCAG CGGTCACCAC ATCCCAGGTC 
AGCGTCACTC GGGGATCTGC CAAAAAGCCC CAGGGCAGAG CGTCCACTCC AGCTGCCGGG 
AAGGCGATGG CTGCCACATC CCTCCAGGCT AAGGTAGTGA CCCCCACCGC CAAGGCGGTT 
GGGGCCGGGA AGCCAGGAGA GAGTTCTGAG AGCAGCGAGG AGTCGGAGAG TGAAGAGGAG 
GCTCCGGCGT CCCAGGGGCA GGTGGAGGCA GCGGGGAGAA CTCCCCGAGC CGTCTCCGTT 
CCGGTCACCC CCATCTCAGT CACGGGGACC CATGAGAGAG TGTCCACGTC GGTGGCCACG 
CAGCCGCAGG CCAAGGCGGG GACTCCCATC AAGGCCTCCG GGGCTGGGAG AGCAGAGAAG 
AGTTCAGAGA GCAGCGAGGA GTCGGAGAGT GAAGAGGAGG CACCGACAGT CAGAGGACAG 
GTGAAGCCAA CGGTCACCGC ATCTCAGGTC AGTGTCACTC CGGGGTCCAT CAAGAAGTTC 
CAAGGCAAAG CATCCACTCC AGCTGCTGGG AAGGGGGTGG CTACCAAGTC CACCCGGGCC 
AAGGTCGTGA CTTCTACCGA TGCCTCGGGG GGCGGGAAGC CAGGGGAGAG TTCTGAGAGC 
AGCGAGGAGT CAGAGAGCGA AGAGGAGACT CCGGCATCCC AGGGGCAGGT GAAGGCAGCG 
GGGAGAACGC CCCGGGCCGT CTCCGTTCCG GTCACCCCCA TCTCAGTCAC GGGGACCTAT 
GAGAGAGTGT CCGCTTCGGT GACCACACAA CCGCAGGCCA AGGCGGGGAC TCCCATCAAG 
GCCTCCGGGG CTGGAAGAGC AGAGAAGAGT TCAGAGAGCA GCGACGAGTC GGAGAGCGAA 
GAGGAGGCAC TGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCACATC CCAGGTCGGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGGGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACACCCCT CCAGGCTAAG GTAGTGACTC CCACCGCCAA GGCGGCTGGG 
GGCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT CGGCGTTACC CCGGGATCTA CCAAAAAGAC CCAGGGCAGA 
GCGTCCACTC CAGCTGCCGG GAGGGCAGTG GTTACCACAC TCCCCCAGAT CAAGGTGAAT 
AAAACCCCTC TGATTTTTGT CGACCCCAAA CGTAGTCTGG CCGGCAAAGC AGCTGCCCCC 
ACCAAAGCCG CCGTGCCTGT CAGCGCCTCA AAGGAGGGGC GGGTCGCCGA AAGTGAGGGC 
AGCAGCTCAT CCGGCAGCGA GGAAGAGGAA GAGGACCTGA TTCCCGCCAC GCAGAATCTG 
GATACTTCCA CGGCAAAACC GACCGTGGTG GCTCTGCCCA TCGCTCGCCG AGGGCCGGTC 
CCCGCTCCCA GGGCCAGCTC GCCTGAGGAC AGCAGCGAGG AGTCTGACTC CGAAGAGGAA 
AAAAGCACAC AGGTGAAGCC CAGCCCGGCT CTGGCCCCGT TGGCCGTGGC AACCGTGAAG 
ACCTCAGGTC AGAAAGCCAG GACGCCACAG CCATCCGTGA AGGGGAGAAA GACCTCGGGG 
TCGGCCCTCG CCCAACTTCC GGCGGCCTTG AAGATTCCCG CTCTGACGAA GGCAGCGCAG 
GATGCTGGGG GATTCCCTGG CTCAACCCCG AGTAGCATCC TCAAAGGAAG CAATGGCAAG 
AAAGCCAAAG GCAAAGCAGG CCAGCCCCAG GCCTCCCCAA CTAAGGCTGC TGGGGCCACC 
CGGAGAGCAG CCCACATCAA CAGCAATAAT TTGCCGGGGA AAAGACAGAA GGAGAATGGG 
GAGGGCGTGA CCAAGGCCAA GTCAGCTCCC AAGAAGGGTG CAGCGCCAAA GGAGGAGGCC 
GGGATGGTGC CAGAGAGCTC TGAGGTGTCC AGCGAGGAGG AAGTGGAGCC TTCGCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCA CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAAAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050345.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906178.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

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CloneID	OOc135256
Clone ID Related Accession (Same CDS sequence)	XM_029050345.1
Accession Version	XM_029050345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT CGGCGTTACC CCGGGATCTA CCAAAAAGAC CCAGGGCAGA 
GCGTCCACTC CAGCTGCCGG GAGGGCAGTG GTTACCACAC TCCCCCAGAT CAAGGTGAAT 
AAAACCCCTC TGATTTTTGT CGACCCCAAA CGTAGTCTGG CCGGCAAAGC AGCTGCCCCC 
ACCAAAGCCG CCGTGCCTGT CAGCGCCTCA AAGGAGGGGC GGGTCGCCGA AAGTGAGGGC 
AGCAGCTCAT CCGGCAGCGA GGAAGAGGAA GAGGACCTGA TTCCCGCCAC GCAGAATCTG 
GATACTTCCA CGGCAAAACC GACCGTGGTG GCTCTGCCCA TCGCTCGCCG AGGGCCGGTC 
CCCGCTCCCA GGGCCAGCTC GCCTGAGGAC AGCAGCGAGG AGTCTGACTC CGAAGAGGAA 
AAAAGCACAC AGGTGAAGCC CAGCCCGGCT CTGGCCCCGT TGGCCGTGGC AACCGTGAAG 
ACCTCAGGTC AGAAAGCCAG GACGCCACAG CCATCCGTGA AGGGGAGAAA GACCTCGGGG 
TCGGCCCTCG CCCAACTTCC GGCGGCCTTG AAGATTCCCG CTCTGACGAA GGCAGCGCAG 
GATGCTGGGG GATTCCCTGG CTCAACCCCG AGTAGCATCC TCAAAGGAAG CAATGGCAAG 
AAAGCCAAAG GCAAAGCAGG CCAGCCCCAG GCCTCCCCAA CTAAGGCTGC TGGGGCCACC 
CGGAGAGCAG CCCACATCAA CAGCAATAAT TTGCCGGGGA AAAGACAGAA GGAGAATGGG 
GAGGGCGTGA CCAAGGCCAA GTCAGCTCCC AAGAAGGGTG CAGCGCCAAA GGAGGAGGCC 
GGGATGGTGC CAGAGAGCTC TGAGGTGTCC AGCGAGGAGG AAGTGGAGCC TTCGCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCA CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAAAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906178.1
CDS	41..5608
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050345.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050345.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT CGGCGTTACC CCGGGATCTA CCAAAAAGAC CCAGGGCAGA 
GCGTCCACTC CAGCTGCCGG GAGGGCAGTG GTTACCACAC TCCCCCAGAT CAAGGTGAAT 
AAAACCCCTC TGATTTTTGT CGACCCCAAA CGTAGTCTGG CCGGCAAAGC AGCTGCCCCC 
ACCAAAGCCG CCGTGCCTGT CAGCGCCTCA AAGGAGGGGC GGGTCGCCGA AAGTGAGGGC 
AGCAGCTCAT CCGGCAGCGA GGAAGAGGAA GAGGACCTGA TTCCCGCCAC GCAGAATCTG 
GATACTTCCA CGGCAAAACC GACCGTGGTG GCTCTGCCCA TCGCTCGCCG AGGGCCGGTC 
CCCGCTCCCA GGGCCAGCTC GCCTGAGGAC AGCAGCGAGG AGTCTGACTC CGAAGAGGAA 
AAAAGCACAC AGGTGAAGCC CAGCCCGGCT CTGGCCCCGT TGGCCGTGGC AACCGTGAAG 
ACCTCAGGTC AGAAAGCCAG GACGCCACAG CCATCCGTGA AGGGGAGAAA GACCTCGGGG 
TCGGCCCTCG CCCAACTTCC GGCGGCCTTG AAGATTCCCG CTCTGACGAA GGCAGCGCAG 
GATGCTGGGG GATTCCCTGG CTCAACCCCG AGTAGCATCC TCAAAGGAAG CAATGGCAAG 
AAAGCCAAAG GCAAAGCAGG CCAGCCCCAG GCCTCCCCAA CTAAGGCTGC TGGGGCCACC 
CGGAGAGCAG CCCACATCAA CAGCAATAAT TTGCCGGGGA AAAGACAGAA GGAGAATGGG 
GAGGGCGTGA CCAAGGCCAA GTCAGCTCCC AAGAAGGGTG CAGCGCCAAA GGAGGAGGCC 
GGGATGGTGC CAGAGAGCTC TGAGGTGTCC AGCGAGGAGG AAGTGGAGCC TTCGCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCA CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAAAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050346.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906179.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OOc135257
Clone ID Related Accession (Same CDS sequence)	XM_029050346.1
Accession Version	XM_029050346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT GAATAAAACC CCTCTGATTT TTGTCGACCC CAAACGTAGT 
CTGGCCGGCA AAGCAGCTGC CCCCACCAAA GCCGCCGTGC CTGTCAGCGC CTCAAAGGAG 
GGGCGGGTCG CCGAAAGTGA GGGCAGCAGC TCATCCGGCA GCGAGGAAGA GGAAGAGGAC 
CTGATTCCCG CCACGCAGAA TCTGGATACT TCCACGGCAA AACCGACCGT GGTGGCTCTG 
CCCATCGCTC GCCGAGGGCC GGTCCCCGCT CCCAGGGCCA GCTCGCCTGA GGACAGCAGC 
GAGGAGTCTG ACTCCGAAGA GGAAAAAAGC ACACAGGTGA AGCCCAGCCC GGCTCTGGCC 
CCGTTGGCCG TGGCAACCGT GAAGACCTCA GGTCAGAAAG CCAGGACGCC ACAGCCATCC 
GTGAAGGGGA GAAAGACCTC GGGGTCGGCC CTCGCCCAAC TTCCGGCGGC CTTGAAGATT 
CCCGCTCTGA CGAAGGCAGC GCAGGATGCT GGGGGATTCC CTGGCTCAAC CCCGAGTAGC 
ATCCTCAAAG GAAGCAATGG CAAGAAAGCC AAAGGCAAAG CAGGCCAGCC CCAGGCCTCC 
CCAACTAAGG CTGCTGGGGC CACCCGGAGA GCAGCCCACA TCAACAGCAA TAATTTGCCG 
GGGAAAAGAC AGAAGGAGAA TGGGGAGGGC GTGACCAAGG CCAAGTCAGC TCCCAAGAAG 
GGTGCAGCGC CAAAGGAGGA GGCCGGGATG GTGCCAGAGA GCTCTGAGGT GTCCAGCGAG 
GAGGAAGTGG AGCCTTCGCA GTCCCTGCTG TCAGGCTCTC CGGCTCCCCG ACTCCCAGGG 
TCGACTCCCA AGTCCACTCC GACCGTGCCT GCCAAGGCCC AGCCCCCAGA GACGCTTCCC 
CGGGGCCCTC CCAAGCCCGA AGTCGGGAAG ACCACCGCCG CCAACGACCC TCTGGGAGTG 
ATAGTCCCTG CCAGCTCCGC CACCGACGAC AGCAGCGACG GAGGCAGCTC CGAGGACGAC 
GGCAAGCCGA CGGCGGTCAA GCCGCCCGTA GGAACAGGAA GGGAGACCTC TTGTGAAAAG 
GAGACGCCCG TCGCCCCCAG GCACCCGGTC ACAGTCCCCA ACTCGGCCCC ACCCGTCCCC 
ACCTCCAACC ACAAGAGCAC CCCGGGGACT AACGCTGCCT ACGCTCTGCT GTCCCTGGCG 
AGGGCCGGGG CCGAGAGCTC CGGGGACGGG GAACTGGACA GCGAGGAAGA GGCCTTGGCC 
ACGTCCAGGG CCCAGGCCTC GGTGGTGAAG GTGCTGACGG ATCTGCTGCA GCAGGAGAAG 
AAGAAAGCCA CTAAGATCAT CAAGGCTGCC AAGTCACCCA AGGTCAAGGA GGGCAAGAAG 
CAGAAATCCT CCCAGAAACG GAAACTGGCA GGGGAGGACG GGGACACCAA GGCAACCGGG 
AACAAGAAAC AGAAGCTGGC AACGGAGGAC TCTCAGGAGG GAGAGGCCAA GGCAGGCAAA 
GGCGGCAGGA CTAAGAAAGG GAAAAAAGAA AAGGGGACGG GGGATGCCCA GGAAAAGAGG 
GTCAGAGGGG CATCGGGCAC CAAGAAATCC AAAGAGAAGT CGGGCGACGA CCTGGACCCC 
ACAAAGGTGA GGAGCGAGGA TGCCGGAGAC ACAAAGACCA AGAAAGAAAA AAAGGAGAAG 
AAGAAATCTG ATAAGAAAGA CAAGACCCCC AAGGACAAGA AGAAACGGAA AAAGTCGTTG 
TCCAAAGACT CGGATTCCTC GGTGCTGAAG AAAAAAAAGA AAAAGGAAAA GAAAGTCGAC 
CAGTCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906179.1
CDS	40..5511
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050346.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050346.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT GAATAAAACC CCTCTGATTT TTGTCGACCC CAAACGTAGT 
CTGGCCGGCA AAGCAGCTGC CCCCACCAAA GCCGCCGTGC CTGTCAGCGC CTCAAAGGAG 
GGGCGGGTCG CCGAAAGTGA GGGCAGCAGC TCATCCGGCA GCGAGGAAGA GGAAGAGGAC 
CTGATTCCCG CCACGCAGAA TCTGGATACT TCCACGGCAA AACCGACCGT GGTGGCTCTG 
CCCATCGCTC GCCGAGGGCC GGTCCCCGCT CCCAGGGCCA GCTCGCCTGA GGACAGCAGC 
GAGGAGTCTG ACTCCGAAGA GGAAAAAAGC ACACAGGTGA AGCCCAGCCC GGCTCTGGCC 
CCGTTGGCCG TGGCAACCGT GAAGACCTCA GGTCAGAAAG CCAGGACGCC ACAGCCATCC 
GTGAAGGGGA GAAAGACCTC GGGGTCGGCC CTCGCCCAAC TTCCGGCGGC CTTGAAGATT 
CCCGCTCTGA CGAAGGCAGC GCAGGATGCT GGGGGATTCC CTGGCTCAAC CCCGAGTAGC 
ATCCTCAAAG GAAGCAATGG CAAGAAAGCC AAAGGCAAAG CAGGCCAGCC CCAGGCCTCC 
CCAACTAAGG CTGCTGGGGC CACCCGGAGA GCAGCCCACA TCAACAGCAA TAATTTGCCG 
GGGAAAAGAC AGAAGGAGAA TGGGGAGGGC GTGACCAAGG CCAAGTCAGC TCCCAAGAAG 
GGTGCAGCGC CAAAGGAGGA GGCCGGGATG GTGCCAGAGA GCTCTGAGGT GTCCAGCGAG 
GAGGAAGTGG AGCCTTCGCA GTCCCTGCTG TCAGGCTCTC CGGCTCCCCG ACTCCCAGGG 
TCGACTCCCA AGTCCACTCC GACCGTGCCT GCCAAGGCCC AGCCCCCAGA GACGCTTCCC 
CGGGGCCCTC CCAAGCCCGA AGTCGGGAAG ACCACCGCCG CCAACGACCC TCTGGGAGTG 
ATAGTCCCTG CCAGCTCCGC CACCGACGAC AGCAGCGACG GAGGCAGCTC CGAGGACGAC 
GGCAAGCCGA CGGCGGTCAA GCCGCCCGTA GGAACAGGAA GGGAGACCTC TTGTGAAAAG 
GAGACGCCCG TCGCCCCCAG GCACCCGGTC ACAGTCCCCA ACTCGGCCCC ACCCGTCCCC 
ACCTCCAACC ACAAGAGCAC CCCGGGGACT AACGCTGCCT ACGCTCTGCT GTCCCTGGCG 
AGGGCCGGGG CCGAGAGCTC CGGGGACGGG GAACTGGACA GCGAGGAAGA GGCCTTGGCC 
ACGTCCAGGG CCCAGGCCTC GGTGGTGAAG GTGCTGACGG ATCTGCTGCA GCAGGAGAAG 
AAGAAAGCCA CTAAGATCAT CAAGGCTGCC AAGTCACCCA AGGTCAAGGA GGGCAAGAAG 
CAGAAATCCT CCCAGAAACG GAAACTGGCA GGGGAGGACG GGGACACCAA GGCAACCGGG 
AACAAGAAAC AGAAGCTGGC AACGGAGGAC TCTCAGGAGG GAGAGGCCAA GGCAGGCAAA 
GGCGGCAGGA CTAAGAAAGG GAAAAAAGAA AAGGGGACGG GGGATGCCCA GGAAAAGAGG 
GTCAGAGGGG CATCGGGCAC CAAGAAATCC AAAGAGAAGT CGGGCGACGA CCTGGACCCC 
ACAAAGGTGA GGAGCGAGGA TGCCGGAGAC ACAAAGACCA AGAAAGAAAA AAAGGAGAAG 
AAGAAATCTG ATAAGAAAGA CAAGACCCCC AAGGACAAGA AGAAACGGAA AAAGTCGTTG 
TCCAAAGACT CGGATTCCTC GGTGCTGAAG AAAAAAAAGA AAAAGGAAAA GAAAGTCGAC 
CAGTCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050347.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906180.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

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CloneID	OOc135258
Clone ID Related Accession (Same CDS sequence)	XM_029050347.1
Accession Version	XM_029050347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT GAAGCCAGCG 
GTCACCGCAT CCCAGGTCAG CGTCCCTCTG GGATCTGCCA AAAAGCCTCA GGGCAGAGCG 
TCCACTCCAG CTGCCGGGAA GGCGATGGCT GCCACATCCC TCCAGGCTAA AATAGTGACT 
CCCACCGCCA AGGCGGTTGG GGCCGGGAAG CCAGGGGAGA GTTCCGAGAG CAGCGAGGAA 
TCGGAGAGTG AAGAGGAGAC TCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAGTCA CGGGGACCCA TGAGAGAGTT 
TCCACTTCGG TGGCCACGCA GCTGCGGACC AAGGCGGGGA CTCCCGTCCA GGCTGAGAAG 
GCGGAGAAGA GTTCAGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAAGC ACCGGCAGTC 
AGAGGAAAGG GGAAGCCAGT GGTCATCGCA TCCCAGGTCG GCATCACTCC GGGATCTGCC 
AGAAAGCCCC AGGGCAGAGT GTCCACTCCA GCTGCCGGGA ACCGGGTGGC TGCCACAGCC 
CCCATGGCTA AGGTAGCGAC TCCTACCACC AAGGCCTCTG GGGGCGGGAA ACCAGGGGAG 
AGTTCTGAGA GCAGCGAGGA GTCGGAGAGC GAAGAAGAGG CGCCGGCGTC CCAGGGGCAG 
GTGAAGGCAG CGGGGAGAAC TCCCCGGGCC GTCTCCGTTC CGGTCACCCC CATCTCAATC 
AGCGGGACCC ATGAGAGAGC GTCCGCTTCG GTGGCCACAC GACCGCAGGC CAAGGCGGGG 
ACTCCCATCA AGGCCTCCGG GGCTGGGAGA GCAGAGAACA GTTCAGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAGGAGGC ACCGGCAGTC CAGGGACCGG TGAAGCCAGC GGTCACCACA 
TCCCAGGTCA GCGTCACTCG GGGATCTGCC AAAAAGCCCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA AGGCGATGGC TGCCACATCC CTCCAGGCTA AGGTAGTGAC CCCCACCGCC 
AAGGCGGTTG GGGCCGGGAA GCCAGGAGAG AGTTCTGAGA GCAGCGAGGA GTCGGAGAGT 
GAAGAGGAGG CTCCGGCGTC CCAGGGGCAG GTGGAGGCAG CGGGGAGAAC TCCCCGAGCC 
GTCTCCGTTC CGGTCACCCC CATCTCAGTC ACGGGGACCC ATGAGAGAGT GTCCACGTCG 
GTGGCCACGC AGCCGCAGGC CAAGGCGGGG ACTCCCATCA AGGCCTCCGG GGCTGGGAGA 
GCAGAGAAGA GTTCAGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC ACCGACAGTC 
AGAGGACAGG TGAAGCCAAC GGTCACCGCA TCTCAGGTCA GTGTCACTCC GGGGTCCATC 
AAGAAGTTCC AAGGCAAAGC ATCCACTCCA GCTGCTGGGA AGGGGGTGGC TACCAAGTCC 
ACCCGGGCCA AGGTCGTGAC TTCTACCGAT GCCTCGGGGG GCGGGAAGCC AGGGGAGAGT 
TCTGAGAGCA GCGAGGAGTC AGAGAGCGAA GAGGAGACTC CGGCATCCCA GGGGCAGGTG 
AAGGCAGCGG GGAGAACGCC CCGGGCCGTC TCCGTTCCGG TCACCCCCAT CTCAGTCACG 
GGGACCTATG AGAGAGTGTC CGCTTCGGTG ACCACACAAC CGCAGGCCAA GGCGGGGACT 
CCCATCAAGG CCTCCGGGGC TGGAAGAGCA GAGAAGAGTT CAGAGAGCAG CGACGAGTCG 
GAGAGCGAAG AGGAGGCACT GGCAGTCCAG GGACCGGTGA AGCCAGCGGT CACCACATCC 
CAGGTCGGCG TCCCTCTGGG ATCTGCCAAA AAGCCTCAGG GCAGAGGGTC CACTCCAGCT 
GCCGGGAAGG CGATGGCTGC CACACCCCTC CAGGCTAAGG TAGTGACTCC CACCGCCAAG 
GCGGCTGGGG GCGGGAAGCC AGGGGAGAGT TCCGAGAGCA GCGAGGAATC GGAGAGTGAA 
GAGGAGACTC CGGCGTCCCA GGGGCAGGTC GGCGTTACCC CGGGATCTAC CAAAAAGACC 
CAGGGCAGAG CGTCCACTCC AGCTGCCGGG AGGGCAGTGG TTACCACACT CCCCCAGATC 
AAGGTGAATA AAACCCCTCT GATTTTTGTC GACCCCAAAC GTAGTCTGGC CGGCAAAGCA 
GCTGCCCCCA CCAAAGCCGC CGTGCCTGTC AGCGCCTCAA AGGAGGGGCG GGTCGCCGAA 
AGTGAGGGCA GCAGCTCATC CGGCAGCGAG GAAGAGGAAG AGGACCTGAT TCCCGCCACG 
CAGAATCTGG ATACTTCCAC GGCAAAACCG ACCGTGGTGG CTCTGCCCAT CGCTCGCCGA 
GGGCCGGTCC CCGCTCCCAG GGCCAGCTCG CCTGAGGACA GCAGCGAGGA GTCTGACTCC 
GAAGAGGAAA AAAGCACACA GGTGAAGCCC AGCCCGGCTC TGGCCCCGTT GGCCGTGGCA 
ACCGTGAAGA CCTCAGGTCA GAAAGCCAGG ACGCCACAGC CATCCGTGAA GGGGAGAAAG 
ACCTCGGGGT CGGCCCTCGC CCAACTTCCG GCGGCCTTGA AGATTCCCGC TCTGACGAAG 
GCAGCGCAGG ATGCTGGGGG ATTCCCTGGC TCAACCCCGA GTAGCATCCT CAAAGGAAGC 
AATGGCAAGA AAGCCAAAGG CAAAGCAGGC CAGCCCCAGG CCTCCCCAAC TAAGGCTGCT 
GGGGCCACCC GGAGAGCAGC CCACATCAAC AGCAATAATT TGCCGGGGAA AAGACAGAAG 
GAGAATGGGG AGGGCGTGAC CAAGGCCAAG TCAGCTCCCA AGAAGGGTGC AGCGCCAAAG 
GAGGAGGCCG GGATGGTGCC AGAGAGCTCT GAGGTGTCCA GCGAGGAGGA AGTGGAGCCT 
TCGCAGTCCC TGCTGTCAGG CTCTCCGGCT CCCCGACTCC CAGGGTCGAC TCCCAAGTCC 
ACTCCGACCG TGCCTGCCAA GGCCCAGCCC CCAGAGACGC TTCCCCGGGG CCCTCCCAAG 
CCCGAAGTCG GGAAGACCAC CGCCGCCAAC GACCCTCTGG GAGTGATAGT CCCTGCCAGC 
TCCGCCACCG ACGACAGCAG CGACGGAGGC AGCTCCGAGG ACGACGGCAA GCCGACGGCG 
GTCAAGCCGC CCGTAGGAAC AGGAAGGGAG ACCTCTTGTG AAAAGGAGAC GCCCGTCGCC 
CCCAGGCACC CGGTCACAGT CCCCAACTCG GCCCCACCCG TCCCCACCTC CAACCACAAG 
AGCACCCCGG GGACTAACGC TGCCTACGCT CTGCTGTCCC TGGCGAGGGC CGGGGCCGAG 
AGCTCCGGGG ACGGGGAACT GGACAGCGAG GAAGAGGCCT TGGCCACGTC CAGGGCCCAG 
GCCTCGGTGG TGAAGGTGCT GACGGATCTG CTGCAGCAGG AGAAGAAGAA AGCCACTAAG 
ATCATCAAGG CTGCCAAGTC ACCCAAGGTC AAGGAGGGCA AGAAGCAGAA ATCCTCCCAG 
AAACGGAAAC TGGCAGGGGA GGACGGGGAC ACCAAGGCAA CCGGGAACAA GAAACAGAAG 
CTGGCAACGG AGGACTCTCA GGAGGGAGAG GCCAAGGCAG GCAAAGGCGG CAGGACTAAG 
AAAGGGAAAA AAGAAAAGGG GACGGGGGAT GCCCAGGAAA AGAGGGTCAG AGGGGCATCG 
GGCACCAAGA AATCCAAAGA GAAGTCGGGC GACGACCTGG ACCCCACAAA GGTGAGGAGC 
GAGGATGCCG GAGACACAAA GACCAAGAAA GAAAAAAAGG AGAAGAAGAA ATCTGATAAG 
AAAGACAAGA CCCCCAAGGA CAAGAAGAAA CGGAAAAAGT CGTTGTCCAA AGACTCGGAT 
TCCTCGGTGC TGAAGAAAAA AAAGAAAAAG GAAAAGAAAG TCGACCAGTC TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906180.1
CDS	40..5496
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050347.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050347.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT GAAGCCAGCG 
GTCACCGCAT CCCAGGTCAG CGTCCCTCTG GGATCTGCCA AAAAGCCTCA GGGCAGAGCG 
TCCACTCCAG CTGCCGGGAA GGCGATGGCT GCCACATCCC TCCAGGCTAA AATAGTGACT 
CCCACCGCCA AGGCGGTTGG GGCCGGGAAG CCAGGGGAGA GTTCCGAGAG CAGCGAGGAA 
TCGGAGAGTG AAGAGGAGAC TCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAGTCA CGGGGACCCA TGAGAGAGTT 
TCCACTTCGG TGGCCACGCA GCTGCGGACC AAGGCGGGGA CTCCCGTCCA GGCTGAGAAG 
GCGGAGAAGA GTTCAGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAAGC ACCGGCAGTC 
AGAGGAAAGG GGAAGCCAGT GGTCATCGCA TCCCAGGTCG GCATCACTCC GGGATCTGCC 
AGAAAGCCCC AGGGCAGAGT GTCCACTCCA GCTGCCGGGA ACCGGGTGGC TGCCACAGCC 
CCCATGGCTA AGGTAGCGAC TCCTACCACC AAGGCCTCTG GGGGCGGGAA ACCAGGGGAG 
AGTTCTGAGA GCAGCGAGGA GTCGGAGAGC GAAGAAGAGG CGCCGGCGTC CCAGGGGCAG 
GTGAAGGCAG CGGGGAGAAC TCCCCGGGCC GTCTCCGTTC CGGTCACCCC CATCTCAATC 
AGCGGGACCC ATGAGAGAGC GTCCGCTTCG GTGGCCACAC GACCGCAGGC CAAGGCGGGG 
ACTCCCATCA AGGCCTCCGG GGCTGGGAGA GCAGAGAACA GTTCAGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAGGAGGC ACCGGCAGTC CAGGGACCGG TGAAGCCAGC GGTCACCACA 
TCCCAGGTCA GCGTCACTCG GGGATCTGCC AAAAAGCCCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA AGGCGATGGC TGCCACATCC CTCCAGGCTA AGGTAGTGAC CCCCACCGCC 
AAGGCGGTTG GGGCCGGGAA GCCAGGAGAG AGTTCTGAGA GCAGCGAGGA GTCGGAGAGT 
GAAGAGGAGG CTCCGGCGTC CCAGGGGCAG GTGGAGGCAG CGGGGAGAAC TCCCCGAGCC 
GTCTCCGTTC CGGTCACCCC CATCTCAGTC ACGGGGACCC ATGAGAGAGT GTCCACGTCG 
GTGGCCACGC AGCCGCAGGC CAAGGCGGGG ACTCCCATCA AGGCCTCCGG GGCTGGGAGA 
GCAGAGAAGA GTTCAGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC ACCGACAGTC 
AGAGGACAGG TGAAGCCAAC GGTCACCGCA TCTCAGGTCA GTGTCACTCC GGGGTCCATC 
AAGAAGTTCC AAGGCAAAGC ATCCACTCCA GCTGCTGGGA AGGGGGTGGC TACCAAGTCC 
ACCCGGGCCA AGGTCGTGAC TTCTACCGAT GCCTCGGGGG GCGGGAAGCC AGGGGAGAGT 
TCTGAGAGCA GCGAGGAGTC AGAGAGCGAA GAGGAGACTC CGGCATCCCA GGGGCAGGTG 
AAGGCAGCGG GGAGAACGCC CCGGGCCGTC TCCGTTCCGG TCACCCCCAT CTCAGTCACG 
GGGACCTATG AGAGAGTGTC CGCTTCGGTG ACCACACAAC CGCAGGCCAA GGCGGGGACT 
CCCATCAAGG CCTCCGGGGC TGGAAGAGCA GAGAAGAGTT CAGAGAGCAG CGACGAGTCG 
GAGAGCGAAG AGGAGGCACT GGCAGTCCAG GGACCGGTGA AGCCAGCGGT CACCACATCC 
CAGGTCGGCG TCCCTCTGGG ATCTGCCAAA AAGCCTCAGG GCAGAGGGTC CACTCCAGCT 
GCCGGGAAGG CGATGGCTGC CACACCCCTC CAGGCTAAGG TAGTGACTCC CACCGCCAAG 
GCGGCTGGGG GCGGGAAGCC AGGGGAGAGT TCCGAGAGCA GCGAGGAATC GGAGAGTGAA 
GAGGAGACTC CGGCGTCCCA GGGGCAGGTC GGCGTTACCC CGGGATCTAC CAAAAAGACC 
CAGGGCAGAG CGTCCACTCC AGCTGCCGGG AGGGCAGTGG TTACCACACT CCCCCAGATC 
AAGGTGAATA AAACCCCTCT GATTTTTGTC GACCCCAAAC GTAGTCTGGC CGGCAAAGCA 
GCTGCCCCCA CCAAAGCCGC CGTGCCTGTC AGCGCCTCAA AGGAGGGGCG GGTCGCCGAA 
AGTGAGGGCA GCAGCTCATC CGGCAGCGAG GAAGAGGAAG AGGACCTGAT TCCCGCCACG 
CAGAATCTGG ATACTTCCAC GGCAAAACCG ACCGTGGTGG CTCTGCCCAT CGCTCGCCGA 
GGGCCGGTCC CCGCTCCCAG GGCCAGCTCG CCTGAGGACA GCAGCGAGGA GTCTGACTCC 
GAAGAGGAAA AAAGCACACA GGTGAAGCCC AGCCCGGCTC TGGCCCCGTT GGCCGTGGCA 
ACCGTGAAGA CCTCAGGTCA GAAAGCCAGG ACGCCACAGC CATCCGTGAA GGGGAGAAAG 
ACCTCGGGGT CGGCCCTCGC CCAACTTCCG GCGGCCTTGA AGATTCCCGC TCTGACGAAG 
GCAGCGCAGG ATGCTGGGGG ATTCCCTGGC TCAACCCCGA GTAGCATCCT CAAAGGAAGC 
AATGGCAAGA AAGCCAAAGG CAAAGCAGGC CAGCCCCAGG CCTCCCCAAC TAAGGCTGCT 
GGGGCCACCC GGAGAGCAGC CCACATCAAC AGCAATAATT TGCCGGGGAA AAGACAGAAG 
GAGAATGGGG AGGGCGTGAC CAAGGCCAAG TCAGCTCCCA AGAAGGGTGC AGCGCCAAAG 
GAGGAGGCCG GGATGGTGCC AGAGAGCTCT GAGGTGTCCA GCGAGGAGGA AGTGGAGCCT 
TCGCAGTCCC TGCTGTCAGG CTCTCCGGCT CCCCGACTCC CAGGGTCGAC TCCCAAGTCC 
ACTCCGACCG TGCCTGCCAA GGCCCAGCCC CCAGAGACGC TTCCCCGGGG CCCTCCCAAG 
CCCGAAGTCG GGAAGACCAC CGCCGCCAAC GACCCTCTGG GAGTGATAGT CCCTGCCAGC 
TCCGCCACCG ACGACAGCAG CGACGGAGGC AGCTCCGAGG ACGACGGCAA GCCGACGGCG 
GTCAAGCCGC CCGTAGGAAC AGGAAGGGAG ACCTCTTGTG AAAAGGAGAC GCCCGTCGCC 
CCCAGGCACC CGGTCACAGT CCCCAACTCG GCCCCACCCG TCCCCACCTC CAACCACAAG 
AGCACCCCGG GGACTAACGC TGCCTACGCT CTGCTGTCCC TGGCGAGGGC CGGGGCCGAG 
AGCTCCGGGG ACGGGGAACT GGACAGCGAG GAAGAGGCCT TGGCCACGTC CAGGGCCCAG 
GCCTCGGTGG TGAAGGTGCT GACGGATCTG CTGCAGCAGG AGAAGAAGAA AGCCACTAAG 
ATCATCAAGG CTGCCAAGTC ACCCAAGGTC AAGGAGGGCA AGAAGCAGAA ATCCTCCCAG 
AAACGGAAAC TGGCAGGGGA GGACGGGGAC ACCAAGGCAA CCGGGAACAA GAAACAGAAG 
CTGGCAACGG AGGACTCTCA GGAGGGAGAG GCCAAGGCAG GCAAAGGCGG CAGGACTAAG 
AAAGGGAAAA AAGAAAAGGG GACGGGGGAT GCCCAGGAAA AGAGGGTCAG AGGGGCATCG 
GGCACCAAGA AATCCAAAGA GAAGTCGGGC GACGACCTGG ACCCCACAAA GGTGAGGAGC 
GAGGATGCCG GAGACACAAA GACCAAGAAA GAAAAAAAGG AGAAGAAGAA ATCTGATAAG 
AAAGACAAGA CCCCCAAGGA CAAGAAGAAA CGGAAAAAGT CGTTGTCCAA AGACTCGGAT 
TCCTCGGTGC TGAAGAAAAA AAAGAAAAAG GAAAAGAAAG TCGACCAGTC TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050348.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906181.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

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CloneID	OOc135259
Clone ID Related Accession (Same CDS sequence)	XM_029050348.1
Accession Version	XM_029050348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCACATC CCAGGTCAGC 
GTCACTCGGG GATCTGCCAA AAAGCCCCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAG GTAGTGACCC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGAGAGAG TTCTGAGAGC AGCGAGGAGT CGGAGAGTGA AGAGGAGGCT 
CCGGCGTCCC AGGGGCAGGT GGAGGCAGCG GGGAGAACTC CCCGAGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTGT CCACGTCGGT GGCCACGCAG 
CCGCAGGCCA AGGCGGGGAC TCCCATCAAG GCCTCCGGGG CTGGGAGAGC AGAGAAGAGT 
TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA GAGGAGGCAC CGACAGTCAG AGGACAGGTG 
AAGCCAACGG TCACCGCATC TCAGGTCAGT GTCACTCCGG GGTCCATCAA GAAGTTCCAA 
GGCAAAGCAT CCACTCCAGC TGCTGGGAAG GGGGTGGCTA CCAAGTCCAC CCGGGCCAAG 
GTCGTGACTT CTACCGATGC CTCGGGGGGC GGGAAGCCAG GGGAGAGTTC TGAGAGCAGC 
GAGGAGTCAG AGAGCGAAGA GGAGACTCCG GCATCCCAGG GGCAGGTGAA GGCAGCGGGG 
AGAACGCCCC GGGCCGTCTC CGTTCCGGTC ACCCCCATCT CAGTCACGGG GACCTATGAG 
AGAGTGTCCG CTTCGGTGAC CACACAACCG CAGGCCAAGG CGGGGACTCC CATCAAGGCC 
TCCGGGGCTG GAAGAGCAGA GAAGAGTTCA GAGAGCAGCG ACGAGTCGGA GAGCGAAGAG 
GAGGCACTGG CAGTCCAGGG ACCGGTGAAG CCAGCGGTCA CCACATCCCA GGTCGGCGTC 
CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC AGAGGGTCCA CTCCAGCTGC CGGGAAGGCG 
ATGGCTGCCA CACCCCTCCA GGCTAAGGTA GTGACTCCCA CCGCCAAGGC GGCTGGGGGC 
GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC GAGGAATCGG AGAGTGAAGA GGAGACTCCG 
GCGTCCCAGG GGCAGGTCGG CGTTACCCCG GGATCTACCA AAAAGACCCA GGGCAGAGCG 
TCCACTCCAG CTGCCGGGAG GGCAGTGGTT ACCACACTCC CCCAGATCAA GGTGAATAAA 
ACCCCTCTGA TTTTTGTCGA CCCCAAACGT AGTCTGGCCG GCAAAGCAGC TGCCCCCACC 
AAAGCCGCCG TGCCTGTCAG CGCCTCAAAG GAGGGGCGGG TCGCCGAAAG TGAGGGCAGC 
AGCTCATCCG GCAGCGAGGA AGAGGAAGAG GACCTGATTC CCGCCACGCA GAATCTGGAT 
ACTTCCACGG CAAAACCGAC CGTGGTGGCT CTGCCCATCG CTCGCCGAGG GCCGGTCCCC 
GCTCCCAGGG CCAGCTCGCC TGAGGACAGC AGCGAGGAGT CTGACTCCGA AGAGGAAAAA 
AGCACACAGG TGAAGCCCAG CCCGGCTCTG GCCCCGTTGG CCGTGGCAAC CGTGAAGACC 
TCAGGTCAGA AAGCCAGGAC GCCACAGCCA TCCGTGAAGG GGAGAAAGAC CTCGGGGTCG 
GCCCTCGCCC AACTTCCGGC GGCCTTGAAG ATTCCCGCTC TGACGAAGGC AGCGCAGGAT 
GCTGGGGGAT TCCCTGGCTC AACCCCGAGT AGCATCCTCA AAGGAAGCAA TGGCAAGAAA 
GCCAAAGGCA AAGCAGGCCA GCCCCAGGCC TCCCCAACTA AGGCTGCTGG GGCCACCCGG 
AGAGCAGCCC ACATCAACAG CAATAATTTG CCGGGGAAAA GACAGAAGGA GAATGGGGAG 
GGCGTGACCA AGGCCAAGTC AGCTCCCAAG AAGGGTGCAG CGCCAAAGGA GGAGGCCGGG 
ATGGTGCCAG AGAGCTCTGA GGTGTCCAGC GAGGAGGAAG TGGAGCCTTC GCAGTCCCTG 
CTGTCAGGCT CTCCGGCTCC CCGACTCCCA GGGTCGACTC CCAAGTCCAC TCCGACCGTG 
CCTGCCAAGG CCCAGCCCCC AGAGACGCTT CCCCGGGGCC CTCCCAAGCC CGAAGTCGGG 
AAGACCACCG CCGCCAACGA CCCTCTGGGA GTGATAGTCC CTGCCAGCTC CGCCACCGAC 
GACAGCAGCG ACGGAGGCAG CTCCGAGGAC GACGGCAAGC CGACGGCGGT CAAGCCGCCC 
GTAGGAACAG GAAGGGAGAC CTCTTGTGAA AAGGAGACGC CCGTCGCCCC CAGGCACCCG 
GTCACAGTCC CCAACTCGGC CCCACCCGTC CCCACCTCCA ACCACAAGAG CACCCCGGGG 
ACTAACGCTG CCTACGCTCT GCTGTCCCTG GCGAGGGCCG GGGCCGAGAG CTCCGGGGAC 
GGGGAACTGG ACAGCGAGGA AGAGGCCTTG GCCACGTCCA GGGCCCAGGC CTCGGTGGTG 
AAGGTGCTGA CGGATCTGCT GCAGCAGGAG AAGAAGAAAG CCACTAAGAT CATCAAGGCT 
GCCAAGTCAC CCAAGGTCAA GGAGGGCAAG AAGCAGAAAT CCTCCCAGAA ACGGAAACTG 
GCAGGGGAGG ACGGGGACAC CAAGGCAACC GGGAACAAGA AACAGAAGCT GGCAACGGAG 
GACTCTCAGG AGGGAGAGGC CAAGGCAGGC AAAGGCGGCA GGACTAAGAA AGGGAAAAAA 
GAAAAGGGGA CGGGGGATGC CCAGGAAAAG AGGGTCAGAG GGGCATCGGG CACCAAGAAA 
TCCAAAGAGA AGTCGGGCGA CGACCTGGAC CCCACAAAGG TGAGGAGCGA GGATGCCGGA 
GACACAAAGA CCAAGAAAGA AAAAAAGGAG AAGAAGAAAT CTGATAAGAA AGACAAGACC 
CCCAAGGACA AGAAGAAACG GAAAAAGTCG TTGTCCAAAG ACTCGGATTC CTCGGTGCTG 
AAGAAAAAAA AGAAAAAGGA AAAGAAAGTC GACCAGTCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906181.1
CDS	41..4825
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTTAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_029050348.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050348.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCACATC CCAGGTCAGC 
GTCACTCGGG GATCTGCCAA AAAGCCCCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAG GTAGTGACCC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGAGAGAG TTCTGAGAGC AGCGAGGAGT CGGAGAGTGA AGAGGAGGCT 
CCGGCGTCCC AGGGGCAGGT GGAGGCAGCG GGGAGAACTC CCCGAGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTGT CCACGTCGGT GGCCACGCAG 
CCGCAGGCCA AGGCGGGGAC TCCCATCAAG GCCTCCGGGG CTGGGAGAGC AGAGAAGAGT 
TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA GAGGAGGCAC CGACAGTCAG AGGACAGGTG 
AAGCCAACGG TCACCGCATC TCAGGTCAGT GTCACTCCGG GGTCCATCAA GAAGTTCCAA 
GGCAAAGCAT CCACTCCAGC TGCTGGGAAG GGGGTGGCTA CCAAGTCCAC CCGGGCCAAG 
GTCGTGACTT CTACCGATGC CTCGGGGGGC GGGAAGCCAG GGGAGAGTTC TGAGAGCAGC 
GAGGAGTCAG AGAGCGAAGA GGAGACTCCG GCATCCCAGG GGCAGGTGAA GGCAGCGGGG 
AGAACGCCCC GGGCCGTCTC CGTTCCGGTC ACCCCCATCT CAGTCACGGG GACCTATGAG 
AGAGTGTCCG CTTCGGTGAC CACACAACCG CAGGCCAAGG CGGGGACTCC CATCAAGGCC 
TCCGGGGCTG GAAGAGCAGA GAAGAGTTCA GAGAGCAGCG ACGAGTCGGA GAGCGAAGAG 
GAGGCACTGG CAGTCCAGGG ACCGGTGAAG CCAGCGGTCA CCACATCCCA GGTCGGCGTC 
CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC AGAGGGTCCA CTCCAGCTGC CGGGAAGGCG 
ATGGCTGCCA CACCCCTCCA GGCTAAGGTA GTGACTCCCA CCGCCAAGGC GGCTGGGGGC 
GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC GAGGAATCGG AGAGTGAAGA GGAGACTCCG 
GCGTCCCAGG GGCAGGTCGG CGTTACCCCG GGATCTACCA AAAAGACCCA GGGCAGAGCG 
TCCACTCCAG CTGCCGGGAG GGCAGTGGTT ACCACACTCC CCCAGATCAA GGTGAATAAA 
ACCCCTCTGA TTTTTGTCGA CCCCAAACGT AGTCTGGCCG GCAAAGCAGC TGCCCCCACC 
AAAGCCGCCG TGCCTGTCAG CGCCTCAAAG GAGGGGCGGG TCGCCGAAAG TGAGGGCAGC 
AGCTCATCCG GCAGCGAGGA AGAGGAAGAG GACCTGATTC CCGCCACGCA GAATCTGGAT 
ACTTCCACGG CAAAACCGAC CGTGGTGGCT CTGCCCATCG CTCGCCGAGG GCCGGTCCCC 
GCTCCCAGGG CCAGCTCGCC TGAGGACAGC AGCGAGGAGT CTGACTCCGA AGAGGAAAAA 
AGCACACAGG TGAAGCCCAG CCCGGCTCTG GCCCCGTTGG CCGTGGCAAC CGTGAAGACC 
TCAGGTCAGA AAGCCAGGAC GCCACAGCCA TCCGTGAAGG GGAGAAAGAC CTCGGGGTCG 
GCCCTCGCCC AACTTCCGGC GGCCTTGAAG ATTCCCGCTC TGACGAAGGC AGCGCAGGAT 
GCTGGGGGAT TCCCTGGCTC AACCCCGAGT AGCATCCTCA AAGGAAGCAA TGGCAAGAAA 
GCCAAAGGCA AAGCAGGCCA GCCCCAGGCC TCCCCAACTA AGGCTGCTGG GGCCACCCGG 
AGAGCAGCCC ACATCAACAG CAATAATTTG CCGGGGAAAA GACAGAAGGA GAATGGGGAG 
GGCGTGACCA AGGCCAAGTC AGCTCCCAAG AAGGGTGCAG CGCCAAAGGA GGAGGCCGGG 
ATGGTGCCAG AGAGCTCTGA GGTGTCCAGC GAGGAGGAAG TGGAGCCTTC GCAGTCCCTG 
CTGTCAGGCT CTCCGGCTCC CCGACTCCCA GGGTCGACTC CCAAGTCCAC TCCGACCGTG 
CCTGCCAAGG CCCAGCCCCC AGAGACGCTT CCCCGGGGCC CTCCCAAGCC CGAAGTCGGG 
AAGACCACCG CCGCCAACGA CCCTCTGGGA GTGATAGTCC CTGCCAGCTC CGCCACCGAC 
GACAGCAGCG ACGGAGGCAG CTCCGAGGAC GACGGCAAGC CGACGGCGGT CAAGCCGCCC 
GTAGGAACAG GAAGGGAGAC CTCTTGTGAA AAGGAGACGC CCGTCGCCCC CAGGCACCCG 
GTCACAGTCC CCAACTCGGC CCCACCCGTC CCCACCTCCA ACCACAAGAG CACCCCGGGG 
ACTAACGCTG CCTACGCTCT GCTGTCCCTG GCGAGGGCCG GGGCCGAGAG CTCCGGGGAC 
GGGGAACTGG ACAGCGAGGA AGAGGCCTTG GCCACGTCCA GGGCCCAGGC CTCGGTGGTG 
AAGGTGCTGA CGGATCTGCT GCAGCAGGAG AAGAAGAAAG CCACTAAGAT CATCAAGGCT 
GCCAAGTCAC CCAAGGTCAA GGAGGGCAAG AAGCAGAAAT CCTCCCAGAA ACGGAAACTG 
GCAGGGGAGG ACGGGGACAC CAAGGCAACC GGGAACAAGA AACAGAAGCT GGCAACGGAG 
GACTCTCAGG AGGGAGAGGC CAAGGCAGGC AAAGGCGGCA GGACTAAGAA AGGGAAAAAA 
GAAAAGGGGA CGGGGGATGC CCAGGAAAAG AGGGTCAGAG GGGCATCGGG CACCAAGAAA 
TCCAAAGAGA AGTCGGGCGA CGACCTGGAC CCCACAAAGG TGAGGAGCGA GGATGCCGGA 
GACACAAAGA CCAAGAAAGA AAAAAAGGAG AAGAAGAAAT CTGATAAGAA AGACAAGACC 
CCCAAGGACA AGAAGAAACG GAAAAAGTCG TTGTCCAAAG ACTCGGATTC CTCGGTGCTG 
AAGAAAAAAA AGAAAAAGGA AAAGAAAGTC GACCAGTCTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_029050349.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028906182.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

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CloneID	OOc135260
Clone ID Related Accession (Same CDS sequence)	XM_029050349.1
Accession Version	XM_029050349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTCG GCGTTACCCC GGGATCTACC AAAAAGACCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA GGGCAGTGGT TACCACACTC CCCCAGATCA AGGTGAATAA AACCCCTCTG 
ATTTTTGTCG ACCCCAAACG TAGTCTGGCC GGCAAAGCAG CTGCCCCCAC CAAAGCCGCC 
GTGCCTGTCA GCGCCTCAAA GGAGGGGCGG GTCGCCGAAA GTGAGGGCAG CAGCTCATCC 
GGCAGCGAGG AAGAGGAAGA GGACCTGATT CCCGCCACGC AGAATCTGGA TACTTCCACG 
GCAAAACCGA CCGTGGTGGC TCTGCCCATC GCTCGCCGAG GGCCGGTCCC CGCTCCCAGG 
GCCAGCTCGC CTGAGGACAG CAGCGAGGAG TCTGACTCCG AAGAGGAAAA AAGCACACAG 
GTGAAGCCCA GCCCGGCTCT GGCCCCGTTG GCCGTGGCAA CCGTGAAGAC CTCAGGTCAG 
AAAGCCAGGA CGCCACAGCC ATCCGTGAAG GGGAGAAAGA CCTCGGGGTC GGCCCTCGCC 
CAACTTCCGG CGGCCTTGAA GATTCCCGCT CTGACGAAGG CAGCGCAGGA TGCTGGGGGA 
TTCCCTGGCT CAACCCCGAG TAGCATCCTC AAAGGAAGCA ATGGCAAGAA AGCCAAAGGC 
AAAGCAGGCC AGCCCCAGGC CTCCCCAACT AAGGCTGCTG GGGCCACCCG GAGAGCAGCC 
CACATCAACA GCAATAATTT GCCGGGGAAA AGACAGAAGG AGAATGGGGA GGGCGTGACC 
AAGGCCAAGT CAGCTCCCAA GAAGGGTGCA GCGCCAAAGG AGGAGGCCGG GATGGTGCCA 
GAGAGCTCTG AGGTGTCCAG CGAGGAGGAA GTGGAGCCTT CGCAGGCGTG CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028906182.1
CDS	43..4719
Translation	MAEAKKRRELLPLIHQHLLQAGYVRAAREVKLQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSDESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRGSTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVNKTPLIFVDPKRSLAGKAAAPTKAAVPVSASKEGRVAESEGSSSSGSEEEEEDLIPATQNLDTSTAKPTVVALPIARRGPVPAPRASSPEDSSEESDSEEEKSTQVKPSPALAPLAVATVKTSGQKARTPQPSVKGRKTSGSALAQLPAALKIPALTKAAQDAGGFPGSTPSSILKGSNGKKAKGKAGQPQASPTKAAGATRRAAHINSNNLPGKRQKENGEGVTKAKSAPKKGAAPKEEAGMVPESSEVSSEEEVEPSQACE

Target ORF information:

RefSeq Version	XM_029050349.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050349.1

ORF Insert Sequence:

ATGGCGGAGG CCAAGAAGAG GCGGGAGCTG CTGCCCCTCA TCCACCAGCA CCTGCTGCAG 
GCGGGCTACG TGAGGGCGGC GCGGGAGGTG AAGCTGCAGA GCGGCCAGAA AACCTTCCCG 
ACACAGCCGG TCACCCTTCT GGACATTTAC ACGCATTGGG AACGAACCTC CGAAGAGGCT 
AAGAAACGCA AGGCCGACGA ACTGGATGAA AACCTGCAGG CCAAGAAGGT CAGGGTCTCT 
GATCCCGTGA GCAGCTCGGA GAGCTCTGAA GTGGAGGAAG AGTACACAGC CAAAGGCATG 
AAAACCACCA GGCCTCTGTT GGCCAAATCA GTGACCTTGA TGCCCGAGAA GAATCTCGCC 
GTGACCAAGG ACAGAGCCAA GGGGAAGAAC AAGAAAGTGA CCGGCAAGGC AGTGAACTCC 
ATATCTCCTC CCACACCCAA CAAGGGGCCA GCTCCCCTGG GGAAACCGGG CCTCGCTACT 
CTGGCCCAGG CTCTCTCCCC CAGATCCAAC CCGGTCTCCA AGAACCAGGC CGCCGACTCC 
AGCAGCAGCT CATCGGACAG CGAGGCCGAA GGGGCCGCCT CCGTAGACAG GCTGCATCCC 
AGACCCGTAG CGGGGCCGGT GGCTCGGAAA CCAGAGAGCA GCAGCAGCGG GGCCTCCGGT 
GACGAAACTG ATATTGAGAC CCAGGTGAAA CCAGGAGTAA AAGCTCAGCT CACCAACACC 
CCAGGGAGAA TGGTGCCAGC ATCCACAGGA CCAAAGGGAG GAACATCTGC TCCAGGCACT 
GGGAAGCCGG TGACCGCATC CCCCCAGGCA AAGGCGGGGA CCCCAGTCAG GATCGCTGGG 
GACGGGAGGC CGGAGGAGAG CTCCGAGAGC AGCGAGGAGT CAGAGAGCGA GGAAGAGATG 
CCAGCCTCCC AGGGACAGGT GAAGCCAGCG GTCACAGCAC CCCACGTCAC CCCGGGGTCC 
ACCAAAAAGC CCCAGGGCAG AGCGTCCACC CCAGCTGCCA GGAAGGCAGT GGCCGCCACG 
ACTCCCAGGG CCCAGATCGG GGCTCCCGGC AAGGCCGTCG AGATGAGGAG GGCAGAGGAG 
AGTTCCGAGA GCAGCGAGGA GTCCGAGGGT GAAGATGAGG TGCCGGCATC CCAGGGACAG 
GTGAAGGCAG CGGGGAGGAC TCCTCTGGTC AGTTCTGTTC CGGTCAGCCC CGTCTCGGTC 
AAGGGGTCCC AGGAGAAAGC GACCGCTCCA GCTCTCCGGA AGCCAGTGAC CCAGCCCTCC 
CAGGCCAAGG TGGGGACGCC CAACGTGGCC ACCAAGGCAG GGGTGGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGGCCCCCG AGGGAAAGGT TTCCCAGGCC 
AGGGCCCCCG CCGGGAGAGG TGCGGTCACG GCCCCAGCGT CCACCAAGGG GGCGGCGGCC 
ACACACCCCC AGGCCCAGGC TAAGAAAGTG GGCAGCAGGA AGCCAGAGGA GAGCTCGGAG 
AGCAGCGAGT CATCGGAGAG CGAGGAGGCA TCGGCATCCA AGGGATCGGT CAAGGCAGCG 
GGGAGAACTC CCCGAGCCCT ATCCGTGCCG GTCACCCCCA TCTCATTCAC GGGGACCCAT 
GAGAGAGTGT CCGCTTCGGT GGCCACACGA CCGCAGGCCA AGGCGGGGAC TCCCATCCAG 
GCCTCCGGGG CTGGGAGAGC AGAGGAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA 
GAGGAGGCAC CGGCAGTCCA GGGACCGGTG AAGCCAGCGG TCACCGCATC CCAGGTCAGC 
GTCCCTCTGG GATCTGCCAA AAAGCCTCAG GGCAGAGCGT CCACTCCAGC TGCCGGGAAG 
GCGATGGCTG CCACATCCCT CCAGGCTAAA ATAGTGACTC CCACCGCCAA GGCGGTTGGG 
GCCGGGAAGC CAGGGGAGAG TTCCGAGAGC AGCGAGGAAT CGGAGAGTGA AGAGGAGACT 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAGTCAC GGGGACCCAT GAGAGAGTTT CCACTTCGGT GGCCACGCAG 
CTGCGGACCA AGGCGGGGAC TCCCGTCCAG GCTGAGAAGG CGGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAAGCA CCGGCAGTCA GAGGAAAGGG GAAGCCAGTG 
GTCATCGCAT CCCAGGTCGG CATCACTCCG GGATCTGCCA GAAAGCCCCA GGGCAGAGTG 
TCCACTCCAG CTGCCGGGAA CCGGGTGGCT GCCACAGCCC CCATGGCTAA GGTAGCGACT 
CCTACCACCA AGGCCTCTGG GGGCGGGAAA CCAGGGGAGA GTTCTGAGAG CAGCGAGGAG 
TCGGAGAGCG AAGAAGAGGC GCCGGCGTCC CAGGGGCAGG TGAAGGCAGC GGGGAGAACT 
CCCCGGGCCG TCTCCGTTCC GGTCACCCCC ATCTCAATCA GCGGGACCCA TGAGAGAGCG 
TCCGCTTCGG TGGCCACACG ACCGCAGGCC AAGGCGGGGA CTCCCATCAA GGCCTCCGGG 
GCTGGGAGAG CAGAGAACAG TTCAGAGAGC AGCGAGGAGT CGGAGAGCGA AGAGGAGGCA 
CCGGCAGTCC AGGGACCGGT GAAGCCAGCG GTCACCACAT CCCAGGTCAG CGTCACTCGG 
GGATCTGCCA AAAAGCCCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAA GGCGATGGCT 
GCCACATCCC TCCAGGCTAA GGTAGTGACC CCCACCGCCA AGGCGGTTGG GGCCGGGAAG 
CCAGGAGAGA GTTCTGAGAG CAGCGAGGAG TCGGAGAGTG AAGAGGAGGC TCCGGCGTCC 
CAGGGGCAGG TGGAGGCAGC GGGGAGAACT CCCCGAGCCG TCTCCGTTCC GGTCACCCCC 
ATCTCAGTCA CGGGGACCCA TGAGAGAGTG TCCACGTCGG TGGCCACGCA GCCGCAGGCC 
AAGGCGGGGA CTCCCATCAA GGCCTCCGGG GCTGGGAGAG CAGAGAAGAG TTCAGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCA CCGACAGTCA GAGGACAGGT GAAGCCAACG 
GTCACCGCAT CTCAGGTCAG TGTCACTCCG GGGTCCATCA AGAAGTTCCA AGGCAAAGCA 
TCCACTCCAG CTGCTGGGAA GGGGGTGGCT ACCAAGTCCA CCCGGGCCAA GGTCGTGACT 
TCTACCGATG CCTCGGGGGG CGGGAAGCCA GGGGAGAGTT CTGAGAGCAG CGAGGAGTCA 
GAGAGCGAAG AGGAGACTCC GGCATCCCAG GGGCAGGTGA AGGCAGCGGG GAGAACGCCC 
CGGGCCGTCT CCGTTCCGGT CACCCCCATC TCAGTCACGG GGACCTATGA GAGAGTGTCC 
GCTTCGGTGA CCACACAACC GCAGGCCAAG GCGGGGACTC CCATCAAGGC CTCCGGGGCT 
GGAAGAGCAG AGAAGAGTTC AGAGAGCAGC GACGAGTCGG AGAGCGAAGA GGAGGCACTG 
GCAGTCCAGG GACCGGTGAA GCCAGCGGTC ACCACATCCC AGGTCGGCGT CCCTCTGGGA 
TCTGCCAAAA AGCCTCAGGG CAGAGGGTCC ACTCCAGCTG CCGGGAAGGC GATGGCTGCC 
ACACCCCTCC AGGCTAAGGT AGTGACTCCC ACCGCCAAGG CGGCTGGGGG CGGGAAGCCA 
GGGGAGAGTT CCGAGAGCAG CGAGGAATCG GAGAGTGAAG AGGAGACTCC GGCGTCCCAG 
GGGCAGGTCG GCGTTACCCC GGGATCTACC AAAAAGACCC AGGGCAGAGC GTCCACTCCA 
GCTGCCGGGA GGGCAGTGGT TACCACACTC CCCCAGATCA AGGTGAATAA AACCCCTCTG 
ATTTTTGTCG ACCCCAAACG TAGTCTGGCC GGCAAAGCAG CTGCCCCCAC CAAAGCCGCC 
GTGCCTGTCA GCGCCTCAAA GGAGGGGCGG GTCGCCGAAA GTGAGGGCAG CAGCTCATCC 
GGCAGCGAGG AAGAGGAAGA GGACCTGATT CCCGCCACGC AGAATCTGGA TACTTCCACG 
GCAAAACCGA CCGTGGTGGC TCTGCCCATC GCTCGCCGAG GGCCGGTCCC CGCTCCCAGG 
GCCAGCTCGC CTGAGGACAG CAGCGAGGAG TCTGACTCCG AAGAGGAAAA AAGCACACAG 
GTGAAGCCCA GCCCGGCTCT GGCCCCGTTG GCCGTGGCAA CCGTGAAGAC CTCAGGTCAG 
AAAGCCAGGA CGCCACAGCC ATCCGTGAAG GGGAGAAAGA CCTCGGGGTC GGCCCTCGCC 
CAACTTCCGG CGGCCTTGAA GATTCCCGCT CTGACGAAGG CAGCGCAGGA TGCTGGGGGA 
TTCCCTGGCT CAACCCCGAG TAGCATCCTC AAAGGAAGCA ATGGCAAGAA AGCCAAAGGC 
AAAGCAGGCC AGCCCCAGGC CTCCCCAACT AAGGCTGCTG GGGCCACCCG GAGAGCAGCC 
CACATCAACA GCAATAATTT GCCGGGGAAA AGACAGAAGG AGAATGGGGA GGGCGTGACC 
AAGGCCAAGT CAGCTCCCAA GAAGGGTGCA GCGCCAAAGG AGGAGGCCGG GATGGTGCCA 
GAGAGCTCTG AGGTGTCCAG CGAGGAGGAA GTGGAGCCTT CGCAGGCGTG CGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_016228646.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_016084132.1 Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

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CloneID	OOc38236
Clone ID Related Accession (Same CDS sequence)	XM_016228646.1
Accession Version	XM_016228646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	treacle protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009114.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGTGGGTGC ATAAAATAAA TTTATCTGTC ATAGCTTCAG GCATCGGGTC TTTGGTGAGA 
ATCTGCCACA AAATTCTTAT GGCTATTCCT CTATCCATCC TGGACTCCTG TGGTCCAGAC 
AGGGAAGTGA AGCGGCAGAG CGGGCAGAAA ACCTTCCCGA CACAGCCGGT CACCCTTCTG 
GACATTTACA CGCATTGGGA ACGAACCTCC GAAGAGGCTA AGAAACGCAA GGCCGACGAA 
CTGGATGAAA ACCTGCAGGC CAAGAAGGTC AGGGTCTCTG ATCCCGTGAG CAGCTCGGAG 
AGCTCTGAAG TGGAGGAAGA GTACACAGCC AAAGGCATGA AAACCACCAG GCCTCTGTTG 
GCCAAATCAG TGACCTTGAT GCCCGAGAAG AATCTCGCCG TGACCAAGGA CAGAGCCAAG 
GGGAAGAACA AGAAAGTGAC CGGCAAGGCA GTGAACTCCA TATCTCCTCC CACACCCAAC 
AAGGGGCCAG CTCCCCTGGG GAAACCGGGC CTCGCTACTC TGGCCCAGGC TCTCTCCCCC 
AGATCCAACC CGGTCTCCAA GAACCAGGCC GCCGACTCCA GCAGCAGCTC ATCGGACAGC 
GAGGCCGAAG GGGCCGCCTC CGTAGACAGG CTGCATCCCA GACCCGTAGC GGGGCCGGTG 
GCTCGGAAAC CAGAGAGCAG CAGCAGCGGG GCCTCCGGTG ACGAAACTGA TATTGAGACC 
CAGGTGAAAC CAGGAGTAAA AGCTCAGCTC ACCAACACCC CAGGGAGAAT GGTGCCAGCA 
TCCACAGGAC CAAAGGGAGG AACATCTGCT CCAGGCACTG GGAAGCCGGT GACCGCATCC 
CCCCAGGCAA AGGCGGGGAC CCCAGTCAGG ATCGCTGGGG ACGGGAGGCC GGAGGAGAGC 
TCCGAGAGCA GCGAGGAGTC AGAGAGCGAG GAAGAGATGC CAGCCTCCCA GGGACAGGTG 
AAGCCAGCGG TCACAGCACC CCACGTCACC CCGGGGTCCA CCAAAAAGCC CCAGGGCAGA 
GCGTCCACCC CAGCTGCCAG GAAGGCAGTG GCCGCCACGA CTCCCAGGGC CCAGATCGGG 
GCTCCCGGCA AGGCCGTCGA GATGAGGAGG GCAGAGGAGA GTTCCGAGAG CAGCGAGGAG 
TCCGAGGGTG AAGATGAGGT GCCGGCATCC CAGGGACAGG TGAAGGCAGC GGGGAGGACT 
CCTCTGGTCA GTTCTGTTCC GGTCAGCCCC GTCTCGGTCA AGGGGTCCCA GGAGAAAGCG 
ACCGCTCCAG CTCTCCGGAA GCCAGTGACC CAGCCCTCCC AGGCCAAGGT GGGGACGCCC 
AACGTGGCCA CCAAGGCAGG GGTGGAGAGT TCTGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAGGCAC CGGCCCCCGA GGGAAAGGTT TCCCAGGCCA GGGCCCCCGC CGGGAGAGGT 
GCGGTCACGG CCCCAGCGTC CACCAAGGGG GCGGCGGCCA CACACCCCCA GGCCCAGGCT 
AAGAAAGTGG GCAGCAGGAA GCCAGAGGAG AGCTCGGAGA GCAGCGAGTC ATCGGAGAGC 
GAGGAGGCAT CGGCATCCAA GGGATCGGTC AAGGCAGCGG GGAGAACTCC CCGAGCCCTA 
TCCGTGCCGG TCACCCCCAT CTCATTCACG GGGACCCATG AGAGAGTGTC CGCTTCGGTG 
GCCACACGAC CGCAGGCCAA GGCGGGGACT CCCATCCAGG CCTCCGGGGC TGGGAGAGCA 
GAGGAGAGTT CAGAGAGCAG CGAGGAGTCG GAGAGCGAAG AGGAGGCACC GGCAGTCCAG 
GGACCGGTGA AGCCAGCGGT CACCGCATCC CAGGTCAGCG TCCCTCTGGG ATCTGCCAAA 
AAGCCTCAGG GCAGAGCGTC CACTCCAGCT GCCGGGAAGG CGATGGCTGC CACATCCCTC 
CAGGCTAAAA TAGTGACTCC CACCGCCAAG GCGGTTGGGG CCGGGAAGCC AGGGGAGAGT 
TCCGAGAGCA GCGAGGAATC GGAGAGTGAA GAGGAGACTC CGGCGTCCCA GGGGCAGGTG 
AAGGCAGCGG GGAGAACTCC CCGGGCCGTC TCCGTTCCGG TCACCCCCAT CTCAGTCACG 
GGGACCCATG AGAGAGTTTC CACTTCGGTG GCCACGCAGC TGCGGACCAA GGCGGGGACT 
CCCGTCCAGG CTGAGAAGGC GGAGAAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAAGCAC CGGCAGTCAG AGGAAAGGGG AAGCCAGTGG TCATCGCATC CCAGGTCGGC 
ATCACTCCGG GATCTGCCAG AAAGCCCCAG GGCAGAGTGT CCACTCCAGC TGCCGGGAAC 
CGGGTGGCTG CCACAGCCCC CATGGCTAAG GTAGCGACTC CTACCACCAA GGCCTCTGGG 
GGCGGGAAAC CAGGGGAGAG TTCTGAGAGC AGCGAGGAGT CGGAGAGCGA AGAAGAGGCG 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAATCAG CGGGACCCAT GAGAGAGCGT CCGCTTCGGT GGCCACACGA 
CCGCAGGCCA AGGCGGGGAC TCCCATCAAG GCCTCCGGGG CTGGGAGAGC AGAGAACAGT 
TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA GAGGAGGCAC CGGCAGTCCA GGGACCGGTG 
AAGCCAGCGG TCACCACATC CCAGGTCAGC GTCACTCGGG GATCTGCCAA AAAGCCCCAG 
GGCAGAGCGT CCACTCCAGC TGCCGGGAAG GCGATGGCTG CCACATCCCT CCAGGCTAAG 
GTAGTGACCC CCACCGCCAA GGCGGTTGGG GCCGGGAAGC CAGGAGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCT CCGGCGTCCC AGGGGCAGGT GGAGGCAGCG 
GGGAGAACTC CCCGAGCCGT CTCCGTTCCG GTCACCCCCA TCTCAGTCAC GGGGACCCAT 
GAGAGAGTGT CCACGTCGGT GGCCACGCAG CCGCAGGCCA AGGCGGGGAC TCCCATCAAG 
GCCTCCGGGG CTGGGAGAGC AGAGAAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAGGCAC CGACAGTCAG AGGACAGGTG AAGCCAACGG TCACCGCATC TCAGGTCAGT 
GTCACTCCGG GGTCCATCAA GAAGTTCCAA GGCAAAGCAT CCACTCCAGC TGCTGGGAAG 
GGGGTGGCTA CCAAGTCCAC CCGGGCCAAG GTCGTGACTT CTACCGATGC CTCGGGGGGC 
GGGAAGCCAG GGGAGAGTTC TGAGAGCAGC GAGGAGTCAG AGAGCGAAGA GGAGACTCCG 
GCATCCCAGG GGCAGGTGAA GGCAGCGGGG AGAACGCCCC GGGCCGTCTC CGTTCCGGTC 
ACCCCCATCT CAGTCACGGG GACCTATGAG AGAGTGTCCG CTTCGGTGAC CACACAACCG 
CAGGCCAAGG CGGGGACTCC CATCAAGGCC TCCGGGGCTG GAAGAGCAGA GAAGAGTTCA 
GAGAGCAGCG AGGAGTCGGA GAGCGAAGAG GAGGCACTGG CAGTCCAGGG ACCGGTGAAG 
CCAGCGGTCA CCACATCCCA GGTCGGCGTC CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC 
AGAGCGTCCA CTCCAGCTGC CGGGAAGGCG ATGGCTGCCA CACCCCTCCA GGCTAAGGTA 
GTGACTCCCA CCGCCAAGGC GGCTGGGGGC GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC 
GAGGAATCGG AGAGTGAAGA GGAGACTCCG GCGTCCCAGG GGCAGGTCGG CGTTACCCCG 
GGATCTACCA AAAAGACCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAG GGCAGTGGTT 
ACCACACTCC CCCAGATCAA GGTTGGCATT CCCATCAAAG TCACTGGGGG TGGGAGGCTC 
GGGGAGAGCA GCGACGAGGA GGCAGTGGCT TACAGGGGAT GCCTCTGGCT CTCTCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCG CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAGAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016084132.1
CDS	1..5028
Translation	MWVHKINLSVIASGIGSLVRICHKILMAIPLSILDSCGPDREVKRQSGQKTFPTQPVTLLDIYTHWERTSEEAKKRKADELDENLQAKKVRVSDPVSSSESSEVEEEYTAKGMKTTRPLLAKSVTLMPEKNLAVTKDRAKGKNKKVTGKAVNSISPPTPNKGPAPLGKPGLATLAQALSPRSNPVSKNQAADSSSSSSDSEAEGAASVDRLHPRPVAGPVARKPESSSSGASGDETDIETQVKPGVKAQLTNTPGRMVPASTGPKGGTSAPGTGKPVTASPQAKAGTPVRIAGDGRPEESSESSEESESEEEMPASQGQVKPAVTAPHVTPGSTKKPQGRASTPAARKAVAATTPRAQIGAPGKAVEMRRAEESSESSEESEGEDEVPASQGQVKAAGRTPLVSSVPVSPVSVKGSQEKATAPALRKPVTQPSQAKVGTPNVATKAGVESSESSEESESEEEAPAPEGKVSQARAPAGRGAVTAPASTKGAAATHPQAQAKKVGSRKPEESSESSESSESEEASASKGSVKAAGRTPRALSVPVTPISFTGTHERVSASVATRPQAKAGTPIQASGAGRAEESSESSEESESEEEAPAVQGPVKPAVTASQVSVPLGSAKKPQGRASTPAAGKAMAATSLQAKIVTPTAKAVGAGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTHERVSTSVATQLRTKAGTPVQAEKAEKSSESSEESESEEEAPAVRGKGKPVVIASQVGITPGSARKPQGRVSTPAAGNRVAATAPMAKVATPTTKASGGGKPGESSESSEESESEEEAPASQGQVKAAGRTPRAVSVPVTPISISGTHERASASVATRPQAKAGTPIKASGAGRAENSSESSEESESEEEAPAVQGPVKPAVTTSQVSVTRGSAKKPQGRASTPAAGKAMAATSLQAKVVTPTAKAVGAGKPGESSESSEESESEEEAPASQGQVEAAGRTPRAVSVPVTPISVTGTHERVSTSVATQPQAKAGTPIKASGAGRAEKSSESSEESESEEEAPTVRGQVKPTVTASQVSVTPGSIKKFQGKASTPAAGKGVATKSTRAKVVTSTDASGGGKPGESSESSEESESEEETPASQGQVKAAGRTPRAVSVPVTPISVTGTYERVSASVTTQPQAKAGTPIKASGAGRAEKSSESSEESESEEEALAVQGPVKPAVTTSQVGVPLGSAKKPQGRASTPAAGKAMAATPLQAKVVTPTAKAAGGGKPGESSESSEESESEEETPASQGQVGVTPGSTKKTQGRASTPAAGRAVVTTLPQIKVGIPIKVTGGGRLGESSDEEAVAYRGCLWLSQSLLSGSPAPRLPGSTPKSTPTVPAKAQPPETLPRGPPKPEVGKTAAANDPLGVIVPASSATDDSSDGGSSEDDGKPTAVKPPVGTGRETSCEKETPVAPRHPVTVPNSAPPVPTSNHKSTPGTNAAYALLSLARAGAESSGDGELDSEEEALATSRAQASVVKVLTDLLQQEKKKATKIIKAAKSPKVKEGKKQKSSQKRKLAGEDGDTKATGNKKQKLATEDSQEGEAKAGKGGRTKKGKKEKGTGDAQEKRVRGASGTKKSKEKSGDDLDPTKVRSEDAGDTKTKKEKKEKKKSDKKDKTPKDKKKRKKSLSKDSDSSVLKKKKKKEKKVDQSK

Target ORF information:

RefSeq Version	XM_016228646.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus treacle ribosome biogenesis factor 1 (TCOF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016228646.1

ORF Insert Sequence:

ATGTGGGTGC ATAAAATAAA TTTATCTGTC ATAGCTTCAG GCATCGGGTC TTTGGTGAGA 
ATCTGCCACA AAATTCTTAT GGCTATTCCT CTATCCATCC TGGACTCCTG TGGTCCAGAC 
AGGGAAGTGA AGCGGCAGAG CGGGCAGAAA ACCTTCCCGA CACAGCCGGT CACCCTTCTG 
GACATTTACA CGCATTGGGA ACGAACCTCC GAAGAGGCTA AGAAACGCAA GGCCGACGAA 
CTGGATGAAA ACCTGCAGGC CAAGAAGGTC AGGGTCTCTG ATCCCGTGAG CAGCTCGGAG 
AGCTCTGAAG TGGAGGAAGA GTACACAGCC AAAGGCATGA AAACCACCAG GCCTCTGTTG 
GCCAAATCAG TGACCTTGAT GCCCGAGAAG AATCTCGCCG TGACCAAGGA CAGAGCCAAG 
GGGAAGAACA AGAAAGTGAC CGGCAAGGCA GTGAACTCCA TATCTCCTCC CACACCCAAC 
AAGGGGCCAG CTCCCCTGGG GAAACCGGGC CTCGCTACTC TGGCCCAGGC TCTCTCCCCC 
AGATCCAACC CGGTCTCCAA GAACCAGGCC GCCGACTCCA GCAGCAGCTC ATCGGACAGC 
GAGGCCGAAG GGGCCGCCTC CGTAGACAGG CTGCATCCCA GACCCGTAGC GGGGCCGGTG 
GCTCGGAAAC CAGAGAGCAG CAGCAGCGGG GCCTCCGGTG ACGAAACTGA TATTGAGACC 
CAGGTGAAAC CAGGAGTAAA AGCTCAGCTC ACCAACACCC CAGGGAGAAT GGTGCCAGCA 
TCCACAGGAC CAAAGGGAGG AACATCTGCT CCAGGCACTG GGAAGCCGGT GACCGCATCC 
CCCCAGGCAA AGGCGGGGAC CCCAGTCAGG ATCGCTGGGG ACGGGAGGCC GGAGGAGAGC 
TCCGAGAGCA GCGAGGAGTC AGAGAGCGAG GAAGAGATGC CAGCCTCCCA GGGACAGGTG 
AAGCCAGCGG TCACAGCACC CCACGTCACC CCGGGGTCCA CCAAAAAGCC CCAGGGCAGA 
GCGTCCACCC CAGCTGCCAG GAAGGCAGTG GCCGCCACGA CTCCCAGGGC CCAGATCGGG 
GCTCCCGGCA AGGCCGTCGA GATGAGGAGG GCAGAGGAGA GTTCCGAGAG CAGCGAGGAG 
TCCGAGGGTG AAGATGAGGT GCCGGCATCC CAGGGACAGG TGAAGGCAGC GGGGAGGACT 
CCTCTGGTCA GTTCTGTTCC GGTCAGCCCC GTCTCGGTCA AGGGGTCCCA GGAGAAAGCG 
ACCGCTCCAG CTCTCCGGAA GCCAGTGACC CAGCCCTCCC AGGCCAAGGT GGGGACGCCC 
AACGTGGCCA CCAAGGCAGG GGTGGAGAGT TCTGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAGGCAC CGGCCCCCGA GGGAAAGGTT TCCCAGGCCA GGGCCCCCGC CGGGAGAGGT 
GCGGTCACGG CCCCAGCGTC CACCAAGGGG GCGGCGGCCA CACACCCCCA GGCCCAGGCT 
AAGAAAGTGG GCAGCAGGAA GCCAGAGGAG AGCTCGGAGA GCAGCGAGTC ATCGGAGAGC 
GAGGAGGCAT CGGCATCCAA GGGATCGGTC AAGGCAGCGG GGAGAACTCC CCGAGCCCTA 
TCCGTGCCGG TCACCCCCAT CTCATTCACG GGGACCCATG AGAGAGTGTC CGCTTCGGTG 
GCCACACGAC CGCAGGCCAA GGCGGGGACT CCCATCCAGG CCTCCGGGGC TGGGAGAGCA 
GAGGAGAGTT CAGAGAGCAG CGAGGAGTCG GAGAGCGAAG AGGAGGCACC GGCAGTCCAG 
GGACCGGTGA AGCCAGCGGT CACCGCATCC CAGGTCAGCG TCCCTCTGGG ATCTGCCAAA 
AAGCCTCAGG GCAGAGCGTC CACTCCAGCT GCCGGGAAGG CGATGGCTGC CACATCCCTC 
CAGGCTAAAA TAGTGACTCC CACCGCCAAG GCGGTTGGGG CCGGGAAGCC AGGGGAGAGT 
TCCGAGAGCA GCGAGGAATC GGAGAGTGAA GAGGAGACTC CGGCGTCCCA GGGGCAGGTG 
AAGGCAGCGG GGAGAACTCC CCGGGCCGTC TCCGTTCCGG TCACCCCCAT CTCAGTCACG 
GGGACCCATG AGAGAGTTTC CACTTCGGTG GCCACGCAGC TGCGGACCAA GGCGGGGACT 
CCCGTCCAGG CTGAGAAGGC GGAGAAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAAGCAC CGGCAGTCAG AGGAAAGGGG AAGCCAGTGG TCATCGCATC CCAGGTCGGC 
ATCACTCCGG GATCTGCCAG AAAGCCCCAG GGCAGAGTGT CCACTCCAGC TGCCGGGAAC 
CGGGTGGCTG CCACAGCCCC CATGGCTAAG GTAGCGACTC CTACCACCAA GGCCTCTGGG 
GGCGGGAAAC CAGGGGAGAG TTCTGAGAGC AGCGAGGAGT CGGAGAGCGA AGAAGAGGCG 
CCGGCGTCCC AGGGGCAGGT GAAGGCAGCG GGGAGAACTC CCCGGGCCGT CTCCGTTCCG 
GTCACCCCCA TCTCAATCAG CGGGACCCAT GAGAGAGCGT CCGCTTCGGT GGCCACACGA 
CCGCAGGCCA AGGCGGGGAC TCCCATCAAG GCCTCCGGGG CTGGGAGAGC AGAGAACAGT 
TCAGAGAGCA GCGAGGAGTC GGAGAGCGAA GAGGAGGCAC CGGCAGTCCA GGGACCGGTG 
AAGCCAGCGG TCACCACATC CCAGGTCAGC GTCACTCGGG GATCTGCCAA AAAGCCCCAG 
GGCAGAGCGT CCACTCCAGC TGCCGGGAAG GCGATGGCTG CCACATCCCT CCAGGCTAAG 
GTAGTGACCC CCACCGCCAA GGCGGTTGGG GCCGGGAAGC CAGGAGAGAG TTCTGAGAGC 
AGCGAGGAGT CGGAGAGTGA AGAGGAGGCT CCGGCGTCCC AGGGGCAGGT GGAGGCAGCG 
GGGAGAACTC CCCGAGCCGT CTCCGTTCCG GTCACCCCCA TCTCAGTCAC GGGGACCCAT 
GAGAGAGTGT CCACGTCGGT GGCCACGCAG CCGCAGGCCA AGGCGGGGAC TCCCATCAAG 
GCCTCCGGGG CTGGGAGAGC AGAGAAGAGT TCAGAGAGCA GCGAGGAGTC GGAGAGTGAA 
GAGGAGGCAC CGACAGTCAG AGGACAGGTG AAGCCAACGG TCACCGCATC TCAGGTCAGT 
GTCACTCCGG GGTCCATCAA GAAGTTCCAA GGCAAAGCAT CCACTCCAGC TGCTGGGAAG 
GGGGTGGCTA CCAAGTCCAC CCGGGCCAAG GTCGTGACTT CTACCGATGC CTCGGGGGGC 
GGGAAGCCAG GGGAGAGTTC TGAGAGCAGC GAGGAGTCAG AGAGCGAAGA GGAGACTCCG 
GCATCCCAGG GGCAGGTGAA GGCAGCGGGG AGAACGCCCC GGGCCGTCTC CGTTCCGGTC 
ACCCCCATCT CAGTCACGGG GACCTATGAG AGAGTGTCCG CTTCGGTGAC CACACAACCG 
CAGGCCAAGG CGGGGACTCC CATCAAGGCC TCCGGGGCTG GAAGAGCAGA GAAGAGTTCA 
GAGAGCAGCG AGGAGTCGGA GAGCGAAGAG GAGGCACTGG CAGTCCAGGG ACCGGTGAAG 
CCAGCGGTCA CCACATCCCA GGTCGGCGTC CCTCTGGGAT CTGCCAAAAA GCCTCAGGGC 
AGAGCGTCCA CTCCAGCTGC CGGGAAGGCG ATGGCTGCCA CACCCCTCCA GGCTAAGGTA 
GTGACTCCCA CCGCCAAGGC GGCTGGGGGC GGGAAGCCAG GGGAGAGTTC CGAGAGCAGC 
GAGGAATCGG AGAGTGAAGA GGAGACTCCG GCGTCCCAGG GGCAGGTCGG CGTTACCCCG 
GGATCTACCA AAAAGACCCA GGGCAGAGCG TCCACTCCAG CTGCCGGGAG GGCAGTGGTT 
ACCACACTCC CCCAGATCAA GGTTGGCATT CCCATCAAAG TCACTGGGGG TGGGAGGCTC 
GGGGAGAGCA GCGACGAGGA GGCAGTGGCT TACAGGGGAT GCCTCTGGCT CTCTCAGTCC 
CTGCTGTCAG GCTCTCCGGC TCCCCGACTC CCAGGGTCGA CTCCCAAGTC CACTCCGACC 
GTGCCTGCCA AGGCCCAGCC CCCAGAGACG CTTCCCCGGG GCCCTCCCAA GCCCGAAGTC 
GGGAAGACCG CCGCCGCCAA CGACCCTCTG GGAGTGATAG TCCCTGCCAG CTCCGCCACC 
GACGACAGCA GCGACGGAGG CAGCTCCGAG GACGACGGCA AGCCGACGGC GGTCAAGCCG 
CCCGTAGGAA CAGGAAGGGA GACCTCTTGT GAAAAGGAGA CGCCCGTCGC CCCCAGGCAC 
CCGGTCACAG TCCCCAACTC GGCCCCACCC GTCCCCACCT CCAACCACAA GAGCACCCCG 
GGGACTAACG CTGCCTACGC TCTGCTGTCC CTGGCGAGGG CCGGGGCCGA GAGCTCCGGG 
GACGGGGAAC TGGACAGCGA GGAAGAGGCC TTGGCCACGT CCAGGGCCCA GGCCTCGGTG 
GTGAAGGTGC TGACGGATCT GCTGCAGCAG GAGAAGAAGA AAGCCACTAA GATCATCAAG 
GCTGCCAAGT CACCCAAGGT CAAGGAGGGC AAGAAGCAGA AATCCTCCCA GAAACGGAAA 
CTGGCAGGGG AGGACGGGGA CACCAAGGCA ACCGGGAACA AGAAACAGAA GCTGGCAACG 
GAGGACTCTC AGGAGGGAGA GGCCAAGGCA GGCAAAGGCG GCAGGACTAA GAAAGGGAAA 
AAAGAAAAGG GGACGGGGGA TGCCCAGGAA AAGAGGGTCA GAGGGGCATC GGGCACCAAG 
AAATCCAAAG AGAAGTCGGG CGACGACCTG GACCCCACAA AGGTGAGGAG CGAGGATGCC 
GGAGACACAA AGACCAAGAA AGAAAAGAAG GAGAAGAAGA AATCTGATAA GAAAGACAAG 
ACCCCCAAGG ACAAGAAGAA ACGGAAAAAG TCGTTGTCCA AAGACTCGGA TTCCTCGGTG 
CTGAAGAAAA AAAAGAAAAA GGAAAAGAAA GTCGACCAGT CTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TCOF1
Entrez Gene ID	100079178
Full Name	treacle ribosome biogenesis factor 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TCOF1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

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