AFDN gene cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

mRNA	Protein	Name
XM_019799114.1	XP_019654673.1	afadin isoform X4
XM_019799113.1	XP_019654672.1	afadin isoform X3
XM_019799121.1	XP_019654680.1	afadin isoform X10
XM_019799126.1	XP_019654685.1	afadin isoform X15
XM_019799127.1	XP_019654686.1	afadin isoform X16
XM_019799112.1	XP_019654671.1	afadin isoform X2
XM_019799120.1	XP_019654679.1	afadin isoform X9
XM_019799124.1	XP_019654683.1	afadin isoform X13
XM_019799111.1	XP_019654670.1	afadin isoform X1
XM_019799117.1	XP_019654676.1	afadin isoform X6
XM_019799118.1	XP_019654677.1	afadin isoform X7
XM_019799115.1	XP_019654674.1	afadin isoform X5
XM_019799119.1	XP_019654678.1	afadin isoform X8
XM_019799122.1	XP_019654681.1	afadin isoform X11
XM_019799123.1	XP_019654682.1	afadin isoform X12
XM_019799125.1	XP_019654684.1	afadin isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc92434	XM_019799111.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92435	XM_019799112.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92436	XM_019799113.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92437	XM_019799114.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92438	XM_019799115.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92439	XM_019799117.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92440	XM_019799118.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92441	XM_019799119.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92442	XM_019799120.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92443	XM_019799121.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92444	XM_019799122.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92445	XM_019799123.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92446	XM_019799124.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OAc92447	XM_019799125.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAc92448	XM_019799126.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAc92449	XM_019799127.1 Latest version!	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

AFDN ( XM_019799111.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654670.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc92434
Clone ID Related Accession (Same CDS sequence)	XM_019799111.1
Accession Version	XM_019799111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGTC AGGATAAGCA CTCTTGCACG AGGAAGTCCC TCGGGCACCT TCTTCTTCCC 
GCCGCCCTTA AGCCGCAGCA GCCCCCCTGC CCGCCGCGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCGGCC CGTGCCGACA GTACTGCTCA CAAAGCAGGC 
CAGCCCCCGC CCCCTCCAAA AAAACCAGGC TTCTGCCAGC CCAGCCACTC GAAAGGACCA 
AACTCTTACA TGGGATCGGC TGGGACAGCT GTTGGTGCCC ATGATGCTTA TCGGGATCCG 
AGAGAGAGAC TGTCTAAAAG CCACGATGCA GATTTACCTG GGAGTCCGGG AGCCCCTGAG 
AACTTGACGT TCAGAGAGCG CCAGCGCCTG TTTTCACAAG GTCAAGATGT GTCCAACAAA 
GTAAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654670.1
CDS	18..5651
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799111.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799111.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGTC AGGATAAGCA CTCTTGCACG AGGAAGTCCC TCGGGCACCT TCTTCTTCCC 
GCCGCCCTTA AGCCGCAGCA GCCCCCCTGC CCGCCGCGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCGGCC CGTGCCGACA GTACTGCTCA CAAAGCAGGC 
CAGCCCCCGC CCCCTCCAAA AAAACCAGGC TTCTGCCAGC CCAGCCACTC GAAAGGACCA 
AACTCTTACA TGGGATCGGC TGGGACAGCT GTTGGTGCCC ATGATGCTTA TCGGGATCCG 
AGAGAGAGAC TGTCTAAAAG CCACGATGCA GATTTACCTG GGAGTCCGGG AGCCCCTGAG 
AACTTGACGT TCAGAGAGCG CCAGCGCCTG TTTTCACAAG GTCAAGATGT GTCCAACAAA 
GTAAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799112.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654671.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OAc92435
Clone ID Related Accession (Same CDS sequence)	XM_019799112.1
Accession Version	XM_019799112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG 
TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGTCAGG ATAAGCACTC TTGCACGAGG AAGTCCCTCG GGCACCTTCT TCTTCCCGCC 
GCCCTTAAGC CGCAGCAGCC CCCCTGCCCG CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCGGCCCGT GCCGACAGTA CTGCTCACAA AGCAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ACCAGGCTTC TGCCAGCCCA GCCACTCGAA AGGACCAAAC 
TCTTACATGG GATCGGCTGG GACAGCTGTT GGTGCCCATG ATGCTTATCG GGATCCGAGA 
GAGAGACTGT CTAAAAGCCA CGATGCAGAT TTACCTGGGA GTCCGGGAGC CCCTGAGAAC 
TTGACGTTCA GAGAGCGCCA GCGCCTGTTT TCACAAGGTC AAGATGTGTC CAACAAAGTA 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654671.1
CDS	18..5648
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799112.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799112.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG 
TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGTCAGG ATAAGCACTC TTGCACGAGG AAGTCCCTCG GGCACCTTCT TCTTCCCGCC 
GCCCTTAAGC CGCAGCAGCC CCCCTGCCCG CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCGGCCCGT GCCGACAGTA CTGCTCACAA AGCAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ACCAGGCTTC TGCCAGCCCA GCCACTCGAA AGGACCAAAC 
TCTTACATGG GATCGGCTGG GACAGCTGTT GGTGCCCATG ATGCTTATCG GGATCCGAGA 
GAGAGACTGT CTAAAAGCCA CGATGCAGAT TTACCTGGGA GTCCGGGAGC CCCTGAGAAC 
TTGACGTTCA GAGAGCGCCA GCGCCTGTTT TCACAAGGTC AAGATGTGTC CAACAAAGTA 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799113.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654672.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OAc92436
Clone ID Related Accession (Same CDS sequence)	XM_019799113.1
Accession Version	XM_019799113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG 
TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGTCAGG ATAAGCACTC TTGCACGAGG AAGTCCCTCG GGCACCTTCT TCTTCCCGCC 
GCCCTTAAGC CGCAGCAGCC CCCCTGCCCG CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCGGCCCGT GCCGACAGTA CTGCTCACAA AGCAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ACCAGGCTTC TGCCAGCCCA GCCACTCGAA AGGACCAAAC 
TCTTACATGG GATCGGCTGG GACAGCTGTT GGTGCCCATG ATGCTTATCG GGATCCGAGA 
GAGAGACTGT CTAAAAGCCA CGATGCAGAT TTACCTGGGA GTCCGGGAGC CCCTGAGAAC 
TTGACGTTCA GAGAGCGCCA GCGCCTGTTT TCACAAGGTC AAGATGTGTC CAACAAAGTA 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654672.1
CDS	18..5648
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799113.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799113.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGCAGACT GCTCTGCCAG CAATCTCTGT TCTAGATTTG 
TTACAGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGTCAGG ATAAGCACTC TTGCACGAGG AAGTCCCTCG GGCACCTTCT TCTTCCCGCC 
GCCCTTAAGC CGCAGCAGCC CCCCTGCCCG CCGCGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCGGCCCGT GCCGACAGTA CTGCTCACAA AGCAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ACCAGGCTTC TGCCAGCCCA GCCACTCGAA AGGACCAAAC 
TCTTACATGG GATCGGCTGG GACAGCTGTT GGTGCCCATG ATGCTTATCG GGATCCGAGA 
GAGAGACTGT CTAAAAGCCA CGATGCAGAT TTACCTGGGA GTCCGGGAGC CCCTGAGAAC 
TTGACGTTCA GAGAGCGCCA GCGCCTGTTT TCACAAGGTC AAGATGTGTC CAACAAAGTA 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799114.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654673.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAc92437
Clone ID Related Accession (Same CDS sequence)	XM_019799114.1
Accession Version	XM_019799114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA 
GATCGCGCCA GGCAGGAGGA GGAGCGGCGC CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT 
GCTGAAGAAA AGAGGCGACA AGAAGAAGGT TATTACAGCC GCCTGGAAGC CGAGAGGCGC 
AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA 
CCTCCGCTTC CGCGGGGCTA CGAGCTCCCG TCCCTGTCCC CTCCGCCTGC CGCTCCTCCT 
CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCGCAGG CCCTCTCCCC AGACTCGGTG 
TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGCGGCGG AGGAGCACTG CGGCCTGGCA 
GGTCAGGATA AGCACTCTTG CACGAGGAAG TCCCTCGGGC ACCTTCTTCT TCCCGCCGCC 
CTTAAGCCGC AGCAGCCCCC CTGCCCGCCG CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC GGCCCGTGCC GACAGTACTG CTCACAAAGC AGGCCAGCCC 
CCGCCCCCTC CAAAAAAACC AGGCTTCTGC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT 
TACATGGGAT CGGCTGGGAC AGCTGTTGGT GCCCATGATG CTTATCGGGA TCCGAGAGAG 
AGACTGTCTA AAAGCCACGA TGCAGATTTA CCTGGGAGTC CGGGAGCCCC TGAGAACTTG 
ACGTTCAGAG AGCGCCAGCG CCTGTTTTCA CAAGGTCAAG ATGTGTCCAA CAAAGTAAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654673.1
CDS	18..5645
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799114.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799114.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA 
GATCGCGCCA GGCAGGAGGA GGAGCGGCGC CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT 
GCTGAAGAAA AGAGGCGACA AGAAGAAGGT TATTACAGCC GCCTGGAAGC CGAGAGGCGC 
AGACAGCACG CGGAGGCCGA GCGCAGGTTG CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA 
CCTCCGCTTC CGCGGGGCTA CGAGCTCCCG TCCCTGTCCC CTCCGCCTGC CGCTCCTCCT 
CCCCCGCCTC AGCGGACCGC CTCCTCCCTC CAGGCGCAGG CCCTCTCCCC AGACTCGGTG 
TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGCGGCGG AGGAGCACTG CGGCCTGGCA 
GGTCAGGATA AGCACTCTTG CACGAGGAAG TCCCTCGGGC ACCTTCTTCT TCCCGCCGCC 
CTTAAGCCGC AGCAGCCCCC CTGCCCGCCG CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC GGCCCGTGCC GACAGTACTG CTCACAAAGC AGGCCAGCCC 
CCGCCCCCTC CAAAAAAACC AGGCTTCTGC CAGCCCAGCC ACTCGAAAGG ACCAAACTCT 
TACATGGGAT CGGCTGGGAC AGCTGTTGGT GCCCATGATG CTTATCGGGA TCCGAGAGAG 
AGACTGTCTA AAAGCCACGA TGCAGATTTA CCTGGGAGTC CGGGAGCCCC TGAGAACTTG 
ACGTTCAGAG AGCGCCAGCG CCTGTTTTCA CAAGGTCAAG ATGTGTCCAA CAAAGTAAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799115.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654674.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAc92438
Clone ID Related Accession (Same CDS sequence)	XM_019799115.1
Accession Version	XM_019799115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGACACAG 
AAGAATATCG CAGGGGCGCT TGCCTTCTGG ATGGCCAATG CGTCTGAGCT CCTCAACTTT 
GTCAAACAGG ACCGAGATCT TAGTCGGATC ACACTGGATG CCCAGGACGT TTTAGCACAC 
TTGGTTCAGA TGGCATTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC 
ATGCCAGCCT TTCTGGATGA CCCTGAGGAG AACAGCCTAC AAAGACCAAA AATAGATGAT 
GTTCTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTCAACAGG 
CTGGTGACCG ACCCCGAGTC AGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGAC ACGTGGAAGC CTGGGCAGAG AAGCAGGGCT TGGAGCTGGC CGCCGACTGT 
CACCTCAGCC GGATAGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT 
GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAACT CACTGCAACT GCAAGCCTTG 
TTACAGAATT ACCACTGTGC ACCCGATGAG CCTTTTATCC CCACGGACCT GATAGAAAAT 
GTGGTGGCTG TGGCTGAAAA CACGGCTGAT GAGCTGGCGC GCAGTGACGG GAGGGAGGTG 
CAGCTCGAAG AGGACCCCGA TCTGCAGCTG CCTTTTCTTT TGCCAGAAGA TGGCTATTCT 
TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTGGACCC TTTGTGCCAG 
AGAGGATTTT GCAGGCTCGT CCCTCACGCA CGCTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCACCTTCTG CGCGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAAAAGC AAAATGGGAT GGGCCTCAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTCGGAA TCTATGTCAA GTCTGTTGTA 
AAAGGAGGTG CTGCGGATGT GGATGGACGC CTGGCTGCAG GTGACCAGCT CCTCAGTGTG 
GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGTTCTGTGG TAACACTGGA AGTAGCAAAG CAGGGAGCAA TTTATCACGG CCTCGCCACC 
CTGCTCAATC AGCCGTCCCC GATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCCAAA CGGGTCACCT 
GAGAGCCCTC AGCTGCCCTG GGCAGAATAC AGTGAACCGA AGAAATTGCC AGGCGACGAC 
AGGCTGATGA AGAACCGGGC TGATCACCGC TCTAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGCCCTGGAG GGAAGAGCAC GTACGCCCCC GGAACAACAG CTAAGATAAC GTCTGTCTCC 
ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACCCAGAC 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAAGAGGA ACTTCGGGAG 
GATAAAGCAT ACCAGCTAGA GAGGCATCAA ATCGAGGCAG TCTTGGATCG CAAGTCTGAT 
AGTGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCCA GCACCTCCAG CCAAGAGCAC 
CTGGACCACT CCTCCAAGTC AGGCACCCCC GCGTCCACTC TGACCAAGAG CGGCCCTGGG 
CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC ATGCCCGCGG CCGTCTCCCA GCCTGTCCAA 
ACAGATCCGC CCCCGCCACC GCCACCGCCT CCCACACACT ATGCCGGAGA CTTTGACGGA 
ATCCCCGTGG ACTTGCCTCT CCCTCCGCCT CCTGCCGGCC AGACAGGACC CGCGTCTGCT 
CAGGCGGCGG CCGCAGAGCG GCGGAAGCGG GAGGAGCACC AGCGGTGGTA TGAGAAGGAG 
AAGGCCCGTC TAGAAGAGGA GCGGGACAGG AAGCGCAGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGTCCCT GAACCCGGCC CCGTTCTCTC CTGTGACCGT CCCACACGTG 
AAGCCAGAAA AACCGTCCAC ACTGCAGAGG CCCCAGGAGA CGGTCATTCG GGAGCTGCAG 
CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC GGTCAGCAAA 
GAGGAGCTGT CGTCGGGAGA CAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCACGAG 
CTCCAGAGCA AGCCCGAGCG CAGTGCGGAG GAGAACGACC GCCTGCGGAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG TCCAAACAGA AGGACGAGGA TGACGAGGAA 
GAGGAGGACG ATGACGTGGA TACCATGCTG ATCATGCAGC GCCTTGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGCTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA TGAGGAGCGG 
AGGCGCCAGC AGCAGCTGGA AGAGATGCGC AAGCGGGAAG CCGAAGATCG CGCCAGGCAG 
GAGGAGGAGC GGCGCCGCCA GGAGGAGGAG AGAACAAAGC GAGATGCTGA AGAAAAGAGG 
CGACAAGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGTTGCTGGA GCCGGAGGAG CCGGGTCTGT GCAGACCTCC GCTTCCGCGG 
GGCTACGAGC TCCCGTCCCT GTCCCCTCCG CCTGCCGCTC CTCCTCCCCC GCCTCAGCGG 
ACCGCCTCCT CCCTCCAGGC GCAGGCCCTC TCCCCAGACT CGGTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGC GGCGGAGGAG CACTGCGGCC TGGCAGGTCA GGATAAGCAC 
TCTTGCACGA GGAAGTCCCT CGGGCACCTT CTTCTTCCCG CCGCCCTTAA GCCGCAGCAG 
CCCCCCTGCC CGCCGCGCCC GGCCTCCGAT GGCATCTTTC TCTCCAACTC ATTCCAGCCA 
CCCTCGGCCC GTGCCGACAG TACTGCTCAC AAAGCAGGCC AGCCCCCGCC CCCTCCAAAA 
AAACCAGGCT TCTGCCAGCC CAGCCACTCG AAAGGACCAA ACTCTTACAT GGGATCGGCT 
GGGACAGCTG TTGGTGCCCA TGATGCTTAT CGGGATCCGA GAGAGAGACT GTCTAAAAGC 
CACGATGCAG ATTTACCTGG GAGTCCGGGA GCCCCTGAGA ACTTGACGTT CAGAGAGCGC 
CAGCGCCTGT TTTCACAAGG TCAAGATGTG TCCAACAAAG TAAAAGCCTC TCGTAAATTA 
ACAGAACTGG AAAACGAACT GAACACAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654674.1
CDS	18..5630
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799115.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799115.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGACACAG 
AAGAATATCG CAGGGGCGCT TGCCTTCTGG ATGGCCAATG CGTCTGAGCT CCTCAACTTT 
GTCAAACAGG ACCGAGATCT TAGTCGGATC ACACTGGATG CCCAGGACGT TTTAGCACAC 
TTGGTTCAGA TGGCATTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC 
ATGCCAGCCT TTCTGGATGA CCCTGAGGAG AACAGCCTAC AAAGACCAAA AATAGATGAT 
GTTCTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTCAACAGG 
CTGGTGACCG ACCCCGAGTC AGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGAC ACGTGGAAGC CTGGGCAGAG AAGCAGGGCT TGGAGCTGGC CGCCGACTGT 
CACCTCAGCC GGATAGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT 
GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAACT CACTGCAACT GCAAGCCTTG 
TTACAGAATT ACCACTGTGC ACCCGATGAG CCTTTTATCC CCACGGACCT GATAGAAAAT 
GTGGTGGCTG TGGCTGAAAA CACGGCTGAT GAGCTGGCGC GCAGTGACGG GAGGGAGGTG 
CAGCTCGAAG AGGACCCCGA TCTGCAGCTG CCTTTTCTTT TGCCAGAAGA TGGCTATTCT 
TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTGGACCC TTTGTGCCAG 
AGAGGATTTT GCAGGCTCGT CCCTCACGCA CGCTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCACCTTCTG CGCGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAAAAGC AAAATGGGAT GGGCCTCAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTCGGAA TCTATGTCAA GTCTGTTGTA 
AAAGGAGGTG CTGCGGATGT GGATGGACGC CTGGCTGCAG GTGACCAGCT CCTCAGTGTG 
GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGTTCTGTGG TAACACTGGA AGTAGCAAAG CAGGGAGCAA TTTATCACGG CCTCGCCACC 
CTGCTCAATC AGCCGTCCCC GATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCCAAA CGGGTCACCT 
GAGAGCCCTC AGCTGCCCTG GGCAGAATAC AGTGAACCGA AGAAATTGCC AGGCGACGAC 
AGGCTGATGA AGAACCGGGC TGATCACCGC TCTAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGCCCTGGAG GGAAGAGCAC GTACGCCCCC GGAACAACAG CTAAGATAAC GTCTGTCTCC 
ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACCCAGAC 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAAGAGGA ACTTCGGGAG 
GATAAAGCAT ACCAGCTAGA GAGGCATCAA ATCGAGGCAG TCTTGGATCG CAAGTCTGAT 
AGTGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCCA GCACCTCCAG CCAAGAGCAC 
CTGGACCACT CCTCCAAGTC AGGCACCCCC GCGTCCACTC TGACCAAGAG CGGCCCTGGG 
CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC ATGCCCGCGG CCGTCTCCCA GCCTGTCCAA 
ACAGATCCGC CCCCGCCACC GCCACCGCCT CCCACACACT ATGCCGGAGA CTTTGACGGA 
ATCCCCGTGG ACTTGCCTCT CCCTCCGCCT CCTGCCGGCC AGACAGGACC CGCGTCTGCT 
CAGGCGGCGG CCGCAGAGCG GCGGAAGCGG GAGGAGCACC AGCGGTGGTA TGAGAAGGAG 
AAGGCCCGTC TAGAAGAGGA GCGGGACAGG AAGCGCAGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGTCCCT GAACCCGGCC CCGTTCTCTC CTGTGACCGT CCCACACGTG 
AAGCCAGAAA AACCGTCCAC ACTGCAGAGG CCCCAGGAGA CGGTCATTCG GGAGCTGCAG 
CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC GGTCAGCAAA 
GAGGAGCTGT CGTCGGGAGA CAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCACGAG 
CTCCAGAGCA AGCCCGAGCG CAGTGCGGAG GAGAACGACC GCCTGCGGAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG TCCAAACAGA AGGACGAGGA TGACGAGGAA 
GAGGAGGACG ATGACGTGGA TACCATGCTG ATCATGCAGC GCCTTGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGCTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA TGAGGAGCGG 
AGGCGCCAGC AGCAGCTGGA AGAGATGCGC AAGCGGGAAG CCGAAGATCG CGCCAGGCAG 
GAGGAGGAGC GGCGCCGCCA GGAGGAGGAG AGAACAAAGC GAGATGCTGA AGAAAAGAGG 
CGACAAGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGTTGCTGGA GCCGGAGGAG CCGGGTCTGT GCAGACCTCC GCTTCCGCGG 
GGCTACGAGC TCCCGTCCCT GTCCCCTCCG CCTGCCGCTC CTCCTCCCCC GCCTCAGCGG 
ACCGCCTCCT CCCTCCAGGC GCAGGCCCTC TCCCCAGACT CGGTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGC GGCGGAGGAG CACTGCGGCC TGGCAGGTCA GGATAAGCAC 
TCTTGCACGA GGAAGTCCCT CGGGCACCTT CTTCTTCCCG CCGCCCTTAA GCCGCAGCAG 
CCCCCCTGCC CGCCGCGCCC GGCCTCCGAT GGCATCTTTC TCTCCAACTC ATTCCAGCCA 
CCCTCGGCCC GTGCCGACAG TACTGCTCAC AAAGCAGGCC AGCCCCCGCC CCCTCCAAAA 
AAACCAGGCT TCTGCCAGCC CAGCCACTCG AAAGGACCAA ACTCTTACAT GGGATCGGCT 
GGGACAGCTG TTGGTGCCCA TGATGCTTAT CGGGATCCGA GAGAGAGACT GTCTAAAAGC 
CACGATGCAG ATTTACCTGG GAGTCCGGGA GCCCCTGAGA ACTTGACGTT CAGAGAGCGC 
CAGCGCCTGT TTTCACAAGG TCAAGATGTG TCCAACAAAG TAAAAGCCTC TCGTAAATTA 
ACAGAACTGG AAAACGAACT GAACACAAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799117.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654676.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

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CloneID	OAc92439
Clone ID Related Accession (Same CDS sequence)	XM_019799117.1
Accession Version	XM_019799117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGTTA CAGGATGAGG AGCGGAGGCG CCAGCAGCAG 
CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA GATCGCGCCA GGCAGGAGGA GGAGCGGCGC 
CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT GCTGAAGAAA AGAGGCGACA AGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGTTG 
CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA CCTCCGCTTC CGCGGGGCTA CGAGCTCCCG 
TCCCTGTCCC CTCCGCCTGC CGCTCCTCCT CCCCCGCCTC AGCGGACCGC CTCCTCCCTC 
CAGGCGCAGG CCCTCTCCCC AGACTCGGTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGCGGCGG AGGAGCACTG CGGCCTGGCA GGTCAGGATA AGCACTCTTG CACGAGGAAG 
TCCCTCGGGC ACCTTCTTCT TCCCGCCGCC CTTAAGCCGC AGCAGCCCCC CTGCCCGCCG 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC GGCCCGTGCC 
GACAGTACTG CTCACAAAGC AGGCCAGCCC CCGCCCCCTC CAAAAAAACC AGGCTTCTGC 
CAGCCCAGCC ACTCGAAAGG ACCAAACTCT TACATGGGAT CGGCTGGGAC AGCTGTTGGT 
GCCCATGATG CTTATCGGGA TCCGAGAGAG AGACTGTCTA AAAGCCACGA TGCAGATTTA 
CCTGGGAGTC CGGGAGCCCC TGAGAACTTG ACGTTCAGAG AGCGCCAGCG CCTGTTTTCA 
CAAGGTCAAG ATGTGTCCAA CAAAGTAAAA GCCTCTCGTA AATTAACAGA ACTGGAAAAC 
GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654676.1
CDS	18..5615
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799117.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799117.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGTTA CAGGATGAGG AGCGGAGGCG CCAGCAGCAG 
CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA GATCGCGCCA GGCAGGAGGA GGAGCGGCGC 
CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT GCTGAAGAAA AGAGGCGACA AGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGTTG 
CTGGAGCCGG AGGAGCCGGG TCTGTGCAGA CCTCCGCTTC CGCGGGGCTA CGAGCTCCCG 
TCCCTGTCCC CTCCGCCTGC CGCTCCTCCT CCCCCGCCTC AGCGGACCGC CTCCTCCCTC 
CAGGCGCAGG CCCTCTCCCC AGACTCGGTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGCGGCGG AGGAGCACTG CGGCCTGGCA GGTCAGGATA AGCACTCTTG CACGAGGAAG 
TCCCTCGGGC ACCTTCTTCT TCCCGCCGCC CTTAAGCCGC AGCAGCCCCC CTGCCCGCCG 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC GGCCCGTGCC 
GACAGTACTG CTCACAAAGC AGGCCAGCCC CCGCCCCCTC CAAAAAAACC AGGCTTCTGC 
CAGCCCAGCC ACTCGAAAGG ACCAAACTCT TACATGGGAT CGGCTGGGAC AGCTGTTGGT 
GCCCATGATG CTTATCGGGA TCCGAGAGAG AGACTGTCTA AAAGCCACGA TGCAGATTTA 
CCTGGGAGTC CGGGAGCCCC TGAGAACTTG ACGTTCAGAG AGCGCCAGCG CCTGTTTTCA 
CAAGGTCAAG ATGTGTCCAA CAAAGTAAAA GCCTCTCGTA AATTAACAGA ACTGGAAAAC 
GAACTGAACA CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799118.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654677.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OAc92440
Clone ID Related Accession (Same CDS sequence)	XM_019799118.1
Accession Version	XM_019799118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGGAT GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA 
GAGATGCGCA AGCGGGAAGC CGAAGATCGC GCCAGGCAGG AGGAGGAGCG GCGCCGCCAG 
GAGGAGGAGA GAACAAAGCG AGATGCTGAA GAAAAGAGGC GACAAGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GTTGCTGGAG 
CCGGAGGAGC CGGGTCTGTG CAGACCTCCG CTTCCGCGGG GCTACGAGCT CCCGTCCCTG 
TCCCCTCCGC CTGCCGCTCC TCCTCCCCCG CCTCAGCGGA CCGCCTCCTC CCTCCAGGCG 
CAGGCCCTCT CCCCAGACTC GGTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGCG 
GCGGAGGAGC ACTGCGGCCT GGCAGGTCAG GATAAGCACT CTTGCACGAG GAAGTCCCTC 
GGGCACCTTC TTCTTCCCGC CGCCCTTAAG CCGCAGCAGC CCCCCTGCCC GCCGCGCCCG 
GCCTCCGATG GCATCTTTCT CTCCAACTCA TTCCAGCCAC CCTCGGCCCG TGCCGACAGT 
ACTGCTCACA AAGCAGGCCA GCCCCCGCCC CCTCCAAAAA AACCAGGCTT CTGCCAGCCC 
AGCCACTCGA AAGGACCAAA CTCTTACATG GGATCGGCTG GGACAGCTGT TGGTGCCCAT 
GATGCTTATC GGGATCCGAG AGAGAGACTG TCTAAAAGCC ACGATGCAGA TTTACCTGGG 
AGTCCGGGAG CCCCTGAGAA CTTGACGTTC AGAGAGCGCC AGCGCCTGTT TTCACAAGGT 
CAAGATGTGT CCAACAAAGT AAAAGCCTCT CGTAAATTAA CAGAACTGGA AAACGAACTG 
AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654677.1
CDS	18..5609
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799118.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799118.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGGAT GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA 
GAGATGCGCA AGCGGGAAGC CGAAGATCGC GCCAGGCAGG AGGAGGAGCG GCGCCGCCAG 
GAGGAGGAGA GAACAAAGCG AGATGCTGAA GAAAAGAGGC GACAAGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GTTGCTGGAG 
CCGGAGGAGC CGGGTCTGTG CAGACCTCCG CTTCCGCGGG GCTACGAGCT CCCGTCCCTG 
TCCCCTCCGC CTGCCGCTCC TCCTCCCCCG CCTCAGCGGA CCGCCTCCTC CCTCCAGGCG 
CAGGCCCTCT CCCCAGACTC GGTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGCG 
GCGGAGGAGC ACTGCGGCCT GGCAGGTCAG GATAAGCACT CTTGCACGAG GAAGTCCCTC 
GGGCACCTTC TTCTTCCCGC CGCCCTTAAG CCGCAGCAGC CCCCCTGCCC GCCGCGCCCG 
GCCTCCGATG GCATCTTTCT CTCCAACTCA TTCCAGCCAC CCTCGGCCCG TGCCGACAGT 
ACTGCTCACA AAGCAGGCCA GCCCCCGCCC CCTCCAAAAA AACCAGGCTT CTGCCAGCCC 
AGCCACTCGA AAGGACCAAA CTCTTACATG GGATCGGCTG GGACAGCTGT TGGTGCCCAT 
GATGCTTATC GGGATCCGAG AGAGAGACTG TCTAAAAGCC ACGATGCAGA TTTACCTGGG 
AGTCCGGGAG CCCCTGAGAA CTTGACGTTC AGAGAGCGCC AGCGCCTGTT TTCACAAGGT 
CAAGATGTGT CCAACAAAGT AAAAGCCTCT CGTAAATTAA CAGAACTGGA AAACGAACTG 
AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799119.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654678.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OAc92441
Clone ID Related Accession (Same CDS sequence)	XM_019799119.1
Accession Version	XM_019799119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGACGGCT CTGACTCCAG AGATAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTCACT GAGGTTGGGA CAGAAAAGTT CGATGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGGGTTGTCA CTGTCACGCC AAGAAGCATG GATGCTGAGA CTTATGTGGA AGGACAGCGC 
ATCTCAGAGA CAACCATGCT GCAAAGTGGA ATGAAAGTGC AGTTCGGGGC ATCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCTCTGGCAA AGAGATCTGT GGATGGAGGC 
CTGACAGCCA AGGGTCCCAG ACATAAGCCT GGAATTGTTC AAGAGACAAC TTTTGATTTA 
GGAGGAGATG TTCATAGTGG AACAGCACTG CCAACAAGCA AGAGCACCAC GAGGCTGGAC 
AGCGACAGGG TGTTGTCTGC CTCCAGCACG GCGGAGCGCG GGATGGTGAA GCCAATGATG 
AGAGTAGAAC AGCAGCAGGA TTACCGCAGG CAAGAAAACA GAACTCAGGA CGCCCCTGGG 
CCTGAACTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTA 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACCGTCCATT TTAAGTTGTC ACCTACATAT 
GTATTGTATC TGGCATGTCG GTATGTATTG TCCAGCCAGT CCAGAGCTGA CGTCAGTCCT 
GCAGAGCGCA CTCACAAGGT GATTGCACTG GTCAACAAGA TGGTGAGCAT GATGGAGGGG 
GTGATTCAGG AAGTAGACCA GGTTGATCAG ACACAGAAGA ATATCGCAGG GGCGCTTGCC 
TTCTGGATGG CCAATGCGTC TGAGCTCCTC AACTTTGTCA AACAGGACCG AGATCTTAGT 
CGGATCACAC TGGATGCCCA GGACGTTTTA GCACACTTGG TTCAGATGGC ATTTAAATAT 
CTGGTTCATT GTCTTCAATC AGAACTGAAT AATTACATGC CAGCCTTTCT GGATGACCCT 
GAGGAGAACA GCCTACAAAG ACCAAAAATA GATGATGTTC TGCACACGCT CACGGGAGCC 
ATGTCTTTGC TGCGGCGCTG CAGAGTCAAT GCCGCCCTGA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTC AACAGGCTGG TGACCGACCC CGAGTCAGGA 
CTGTGCTCTC ACTACTGGGG GGCAATCATC CGCCAGCAGC TGGGACACGT GGAAGCCTGG 
GCAGAGAAGC AGGGCTTGGA GCTGGCCGCC GACTGTCACC TCAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACAATGGA TAAATACGCA CCTGATGACA TTCCAAATAT AAACAGTACC 
TGCTTTAAGT TAAACTCACT GCAACTGCAA GCCTTGTTAC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTATCCCCAC GGACCTGATA GAAAATGTGG TGGCTGTGGC TGAAAACACG 
GCTGATGAGC TGGCGCGCAG TGACGGGAGG GAGGTGCAGC TCGAAGAGGA CCCCGATCTG 
CAGCTGCCTT TTCTTTTGCC AGAAGATGGC TATTCTTGTG ATGTTGTCAG GAACATTCCA 
AATGGTTTAC AAGAATTTTT GGACCCTTTG TGCCAGAGAG GATTTTGCAG GCTCGTCCCT 
CACGCACGCT CACCAGGTAC TTGGACGATA TATTTTGAAG GTGCAGATTA TGAGAGCCAC 
CTTCTGCGCG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCTGA AATAATCACT 
GTGACTCTTA AAAAGCAAAA TGGGATGGGC CTCAGCATTG TTGCAGCAAA GGGTGCTGGT 
CAAGATAAAC TCGGAATCTA TGTCAAGTCT GTTGTAAAAG GAGGTGCTGC GGATGTGGAT 
GGACGCCTGG CTGCAGGTGA CCAGCTCCTC AGTGTGGACG GACGAAGTCT GGTAGGACTC 
TCTCAAGAAA GGGCGGCAGA ACTCATGACA AGAACAAGTT CTGTGGTAAC ACTGGAAGTA 
GCAAAGCAGG GAGCAATTTA TCACGGCCTC GCCACCCTGC TCAATCAGCC GTCCCCGATG 
ATGCAAAGAA TTTCAGACCG TCGTGGCTCA GGTAAACCCC GACCAAAGAG TGAAGGTTTC 
GAGCTCTATA ATAATTCAGC TCCAAACGGG TCACCTGAGA GCCCTCAGCT GCCCTGGGCA 
GAATACAGTG AACCGAAGAA ATTGCCAGGC GACGACAGGC TGATGAAGAA CCGGGCTGAT 
CACCGCTCTA GCCCCAACGT AGCAAATCAG CCTCCTAGCC CTGGAGGGAA GAGCACGTAC 
GCCCCCGGAA CAACAGCTAA GATAACGTCT GTCTCCACTG GGAACCTCTG CACTGAGGAA 
CAGACCCCTC CACCTAGACC TGAGGCCTAC CCCATCCCCA CTCAGACGTA CACCAGAGAG 
TATTTCACCT TCCCAGCTTC CAAATCCCAG GACCGGATGG CTCCTCCTCA GAACCAGTGG 
CCAAATTACG AGGAGAAGCC ACACATGCAC CCAGACAGTA ATCATTCCAG TATTGCAATT 
CAGCGTGTTA CCCGTTCCCA AGAGGAACTT CGGGAGGATA AAGCATACCA GCTAGAGAGG 
CATCAAATCG AGGCAGTCTT GGATCGCAAG TCTGATAGTG ATATGTGGAT CCATCAGAGC 
TCCTCAGTGG GCTCCAGCAC CTCCAGCCAA GAGCACCTGG ACCACTCCTC CAAGTCAGGC 
ACCCCCGCGT CCACTCTGAC CAAGAGCGGC CCTGGGCGCT GGAAGACGCC AGCCGCTGTG 
CAGCCCATGC CCGCGGCCGT CTCCCAGCCT GTCCAAACAG ATCCGCCCCC GCCACCGCCA 
CCGCCTCCCA CACACTATGC CGGAGACTTT GACGGAATCC CCGTGGACTT GCCTCTCCCT 
CCGCCTCCTG CCGGCCAGAC AGGACCCGCG TCTGCTCAGG CGGCGGCCGC AGAGCGGCGG 
AAGCGGGAGG AGCACCAGCG GTGGTATGAG AAGGAGAAGG CCCGTCTAGA AGAGGAGCGG 
GACAGGAAGC GCAGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GTCCCTGAAC 
CCGGCCCCGT TCTCTCCTGT GACCGTCCCA CACGTGAAGC CAGAAAAACC GTCCACACTG 
CAGAGGCCCC AGGAGACGGT CATTCGGGAG CTGCAGCCCC AGCAGCAGCC CCGCACGATT 
GAGCGCAGAG ACTTACAGTA CATTACGGTC AGCAAAGAGG AGCTGTCGTC GGGAGACAGT 
CTGTCCCCAG ACCCGTGGAA GCGGGATGCC AGGGAGAAGC TGGAGAAGCA GCAGCAGATG 
CACATCGTGG ATATGCTGAG CAGGGAGATC CACGAGCTCC AGAGCAAGCC CGAGCGCAGT 
GCGGAGGAGA ACGACCGCCT GCGGAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTC 
CAGGAGTCCA AACAGAAGGA CGAGGATGAC GAGGAAGAGG AGGACGATGA CGTGGATACC 
ATGCTGATCA TGCAGCGCCT TGAGGCTGAG CGGAGAGCAA GGCAGACTGC TCTGCCAGCA 
ATCTCTGTTC TAGATTTGTT ACAGGATGAG GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG 
ATGCGCAAGC GGGAAGCCGA AGATCGCGCC AGGCAGGAGG AGGAGCGGCG CCGCCAGGAG 
GAGGAGAGAA CAAAGCGAGA TGCTGAAGAA AAGAGGCGAC AAGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG 
GAGGAGCCGG GTCTGTGCAG ACCTCCGCTT CCGCGGGGCT ACGAGCTCCC GTCCCTGTCC 
CCTCCGCCTG CCGCTCCTCC TCCCCCGCCT CAGCGGACCG CCTCCTCCCT CCAGGCGCAG 
GCCCTCTCCC CAGACTCGGT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGCGGCG 
GAGGAGCACT GCGGCCTGGC AGGTCAGGAT AAGCACTCTT GCACGAGGAA GTCCCTCGGG 
CACCTTCTTC TTCCCGCCGC CCTTAAGCCG CAGCAGCCCC CCTGCCCGCC GCGCCCGGCC 
TCCGATGGCA TCTTTCTCTC CAACTCATTC CAGCCACCCT CGGCCCGTGC CGACAGTACT 
GCTCACAAAG CAGGCCAGCC CCCGCCCCCT CCAAAAAAAC CAGGCTTCTG CCAGCCCAGC 
CACTCGAAAG GACCAAACTC TTACATGGGA TCGGCTGGGA CAGCTGTTGG TGCCCATGAT 
GCTTATCGGG ATCCGAGAGA GAGACTGTCT AAAAGCCACG ATGCAGATTT ACCTGGGAGT 
CCGGGAGCCC CTGAGAACTT GACGTTCAGA GAGCGCCAGC GCCTGTTTTC ACAAGGTCAA 
GATGTGTCCA ACAAAGTAAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654678.1
CDS	18..5606
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799119.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799119.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGACGGCT CTGACTCCAG AGATAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTCACT GAGGTTGGGA CAGAAAAGTT CGATGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGGGTTGTCA CTGTCACGCC AAGAAGCATG GATGCTGAGA CTTATGTGGA AGGACAGCGC 
ATCTCAGAGA CAACCATGCT GCAAAGTGGA ATGAAAGTGC AGTTCGGGGC ATCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCTCTGGCAA AGAGATCTGT GGATGGAGGC 
CTGACAGCCA AGGGTCCCAG ACATAAGCCT GGAATTGTTC AAGAGACAAC TTTTGATTTA 
GGAGGAGATG TTCATAGTGG AACAGCACTG CCAACAAGCA AGAGCACCAC GAGGCTGGAC 
AGCGACAGGG TGTTGTCTGC CTCCAGCACG GCGGAGCGCG GGATGGTGAA GCCAATGATG 
AGAGTAGAAC AGCAGCAGGA TTACCGCAGG CAAGAAAACA GAACTCAGGA CGCCCCTGGG 
CCTGAACTGA TACTGCCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTA 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACCGTCCATT TTAAGTTGTC ACCTACATAT 
GTATTGTATC TGGCATGTCG GTATGTATTG TCCAGCCAGT CCAGAGCTGA CGTCAGTCCT 
GCAGAGCGCA CTCACAAGGT GATTGCACTG GTCAACAAGA TGGTGAGCAT GATGGAGGGG 
GTGATTCAGG AAGTAGACCA GGTTGATCAG ACACAGAAGA ATATCGCAGG GGCGCTTGCC 
TTCTGGATGG CCAATGCGTC TGAGCTCCTC AACTTTGTCA AACAGGACCG AGATCTTAGT 
CGGATCACAC TGGATGCCCA GGACGTTTTA GCACACTTGG TTCAGATGGC ATTTAAATAT 
CTGGTTCATT GTCTTCAATC AGAACTGAAT AATTACATGC CAGCCTTTCT GGATGACCCT 
GAGGAGAACA GCCTACAAAG ACCAAAAATA GATGATGTTC TGCACACGCT CACGGGAGCC 
ATGTCTTTGC TGCGGCGCTG CAGAGTCAAT GCCGCCCTGA CCATCCAGCT CTTCTCCCAG 
CTCTTCCACT TCGTCAACAT GTGGCTCTTC AACAGGCTGG TGACCGACCC CGAGTCAGGA 
CTGTGCTCTC ACTACTGGGG GGCAATCATC CGCCAGCAGC TGGGACACGT GGAAGCCTGG 
GCAGAGAAGC AGGGCTTGGA GCTGGCCGCC GACTGTCACC TCAGCCGGAT AGTGCAGGCA 
ACCACTTTGC TTACAATGGA TAAATACGCA CCTGATGACA TTCCAAATAT AAACAGTACC 
TGCTTTAAGT TAAACTCACT GCAACTGCAA GCCTTGTTAC AGAATTACCA CTGTGCACCC 
GATGAGCCTT TTATCCCCAC GGACCTGATA GAAAATGTGG TGGCTGTGGC TGAAAACACG 
GCTGATGAGC TGGCGCGCAG TGACGGGAGG GAGGTGCAGC TCGAAGAGGA CCCCGATCTG 
CAGCTGCCTT TTCTTTTGCC AGAAGATGGC TATTCTTGTG ATGTTGTCAG GAACATTCCA 
AATGGTTTAC AAGAATTTTT GGACCCTTTG TGCCAGAGAG GATTTTGCAG GCTCGTCCCT 
CACGCACGCT CACCAGGTAC TTGGACGATA TATTTTGAAG GTGCAGATTA TGAGAGCCAC 
CTTCTGCGCG AGAGCACGGA GCTGGCTCAG CCTCTGAGGA AAGAACCTGA AATAATCACT 
GTGACTCTTA AAAAGCAAAA TGGGATGGGC CTCAGCATTG TTGCAGCAAA GGGTGCTGGT 
CAAGATAAAC TCGGAATCTA TGTCAAGTCT GTTGTAAAAG GAGGTGCTGC GGATGTGGAT 
GGACGCCTGG CTGCAGGTGA CCAGCTCCTC AGTGTGGACG GACGAAGTCT GGTAGGACTC 
TCTCAAGAAA GGGCGGCAGA ACTCATGACA AGAACAAGTT CTGTGGTAAC ACTGGAAGTA 
GCAAAGCAGG GAGCAATTTA TCACGGCCTC GCCACCCTGC TCAATCAGCC GTCCCCGATG 
ATGCAAAGAA TTTCAGACCG TCGTGGCTCA GGTAAACCCC GACCAAAGAG TGAAGGTTTC 
GAGCTCTATA ATAATTCAGC TCCAAACGGG TCACCTGAGA GCCCTCAGCT GCCCTGGGCA 
GAATACAGTG AACCGAAGAA ATTGCCAGGC GACGACAGGC TGATGAAGAA CCGGGCTGAT 
CACCGCTCTA GCCCCAACGT AGCAAATCAG CCTCCTAGCC CTGGAGGGAA GAGCACGTAC 
GCCCCCGGAA CAACAGCTAA GATAACGTCT GTCTCCACTG GGAACCTCTG CACTGAGGAA 
CAGACCCCTC CACCTAGACC TGAGGCCTAC CCCATCCCCA CTCAGACGTA CACCAGAGAG 
TATTTCACCT TCCCAGCTTC CAAATCCCAG GACCGGATGG CTCCTCCTCA GAACCAGTGG 
CCAAATTACG AGGAGAAGCC ACACATGCAC CCAGACAGTA ATCATTCCAG TATTGCAATT 
CAGCGTGTTA CCCGTTCCCA AGAGGAACTT CGGGAGGATA AAGCATACCA GCTAGAGAGG 
CATCAAATCG AGGCAGTCTT GGATCGCAAG TCTGATAGTG ATATGTGGAT CCATCAGAGC 
TCCTCAGTGG GCTCCAGCAC CTCCAGCCAA GAGCACCTGG ACCACTCCTC CAAGTCAGGC 
ACCCCCGCGT CCACTCTGAC CAAGAGCGGC CCTGGGCGCT GGAAGACGCC AGCCGCTGTG 
CAGCCCATGC CCGCGGCCGT CTCCCAGCCT GTCCAAACAG ATCCGCCCCC GCCACCGCCA 
CCGCCTCCCA CACACTATGC CGGAGACTTT GACGGAATCC CCGTGGACTT GCCTCTCCCT 
CCGCCTCCTG CCGGCCAGAC AGGACCCGCG TCTGCTCAGG CGGCGGCCGC AGAGCGGCGG 
AAGCGGGAGG AGCACCAGCG GTGGTATGAG AAGGAGAAGG CCCGTCTAGA AGAGGAGCGG 
GACAGGAAGC GCAGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GTCCCTGAAC 
CCGGCCCCGT TCTCTCCTGT GACCGTCCCA CACGTGAAGC CAGAAAAACC GTCCACACTG 
CAGAGGCCCC AGGAGACGGT CATTCGGGAG CTGCAGCCCC AGCAGCAGCC CCGCACGATT 
GAGCGCAGAG ACTTACAGTA CATTACGGTC AGCAAAGAGG AGCTGTCGTC GGGAGACAGT 
CTGTCCCCAG ACCCGTGGAA GCGGGATGCC AGGGAGAAGC TGGAGAAGCA GCAGCAGATG 
CACATCGTGG ATATGCTGAG CAGGGAGATC CACGAGCTCC AGAGCAAGCC CGAGCGCAGT 
GCGGAGGAGA ACGACCGCCT GCGGAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTC 
CAGGAGTCCA AACAGAAGGA CGAGGATGAC GAGGAAGAGG AGGACGATGA CGTGGATACC 
ATGCTGATCA TGCAGCGCCT TGAGGCTGAG CGGAGAGCAA GGCAGACTGC TCTGCCAGCA 
ATCTCTGTTC TAGATTTGTT ACAGGATGAG GAGCGGAGGC GCCAGCAGCA GCTGGAAGAG 
ATGCGCAAGC GGGAAGCCGA AGATCGCGCC AGGCAGGAGG AGGAGCGGCG CCGCCAGGAG 
GAGGAGAGAA CAAAGCGAGA TGCTGAAGAA AAGAGGCGAC AAGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGTT GCTGGAGCCG 
GAGGAGCCGG GTCTGTGCAG ACCTCCGCTT CCGCGGGGCT ACGAGCTCCC GTCCCTGTCC 
CCTCCGCCTG CCGCTCCTCC TCCCCCGCCT CAGCGGACCG CCTCCTCCCT CCAGGCGCAG 
GCCCTCTCCC CAGACTCGGT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGCGGCG 
GAGGAGCACT GCGGCCTGGC AGGTCAGGAT AAGCACTCTT GCACGAGGAA GTCCCTCGGG 
CACCTTCTTC TTCCCGCCGC CCTTAAGCCG CAGCAGCCCC CCTGCCCGCC GCGCCCGGCC 
TCCGATGGCA TCTTTCTCTC CAACTCATTC CAGCCACCCT CGGCCCGTGC CGACAGTACT 
GCTCACAAAG CAGGCCAGCC CCCGCCCCCT CCAAAAAAAC CAGGCTTCTG CCAGCCCAGC 
CACTCGAAAG GACCAAACTC TTACATGGGA TCGGCTGGGA CAGCTGTTGG TGCCCATGAT 
GCTTATCGGG ATCCGAGAGA GAGACTGTCT AAAAGCCACG ATGCAGATTT ACCTGGGAGT 
CCGGGAGCCC CTGAGAACTT GACGTTCAGA GAGCGCCAGC GCCTGTTTTC ACAAGGTCAA 
GATGTGTCCA ACAAAGTAAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799120.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654679.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OAc92442
Clone ID Related Accession (Same CDS sequence)	XM_019799120.1
Accession Version	XM_019799120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654679.1
CDS	18..5423
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799120.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799120.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGACACA GAAGAATATC GCAGGGGCGC TTGCCTTCTG GATGGCCAAT 
GCGTCTGAGC TCCTCAACTT TGTCAAACAG GACCGAGATC TTAGTCGGAT CACACTGGAT 
GCCCAGGACG TTTTAGCACA CTTGGTTCAG ATGGCATTTA AATATCTGGT TCATTGTCTT 
CAATCAGAAC TGAATAATTA CATGCCAGCC TTTCTGGATG ACCCTGAGGA GAACAGCCTA 
CAAAGACCAA AAATAGATGA TGTTCTGCAC ACGCTCACGG GAGCCATGTC TTTGCTGCGG 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CCCAGCTCTT CCACTTCGTC 
AACATGTGGC TCTTCAACAG GCTGGTGACC GACCCCGAGT CAGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGA CACGTGGAAG CCTGGGCAGA GAAGCAGGGC 
TTGGAGCTGG CCGCCGACTG TCACCTCAGC CGGATAGTGC AGGCAACCAC TTTGCTTACA 
ATGGATAAAT ACGCACCTGA TGACATTCCA AATATAAACA GTACCTGCTT TAAGTTAAAC 
TCACTGCAAC TGCAAGCCTT GTTACAGAAT TACCACTGTG CACCCGATGA GCCTTTTATC 
CCCACGGACC TGATAGAAAA TGTGGTGGCT GTGGCTGAAA ACACGGCTGA TGAGCTGGCG 
CGCAGTGACG GGAGGGAGGT GCAGCTCGAA GAGGACCCCG ATCTGCAGCT GCCTTTTCTT 
TTGCCAGAAG ATGGCTATTC TTGTGATGTT GTCAGGAACA TTCCAAATGG TTTACAAGAA 
TTTTTGGACC CTTTGTGCCA GAGAGGATTT TGCAGGCTCG TCCCTCACGC ACGCTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCACCTTCT GCGCGAGAGC 
ACGGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC TCTTAAAAAG 
CAAAATGGGA TGGGCCTCAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTCGGA 
ATCTATGTCA AGTCTGTTGT AAAAGGAGGT GCTGCGGATG TGGATGGACG CCTGGCTGCA 
GGTGACCAGC TCCTCAGTGT GGACGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCG 
GCAGAACTCA TGACAAGAAC AAGTTCTGTG GTAACACTGG AAGTAGCAAA GCAGGGAGCA 
ATTTATCACG GCCTCGCCAC CCTGCTCAAT CAGCCGTCCC CGATGATGCA AAGAATTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GTTTCGAGCT CTATAATAAT 
TCAGCTCCAA ACGGGTCACC TGAGAGCCCT CAGCTGCCCT GGGCAGAATA CAGTGAACCG 
AAGAAATTGC CAGGCGACGA CAGGCTGATG AAGAACCGGG CTGATCACCG CTCTAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGCCCTGGA GGGAAGAGCA CGTACGCCCC CGGAACAACA 
GCTAAGATAA CGTCTGTCTC CACTGGGAAC CTCTGCACTG AGGAACAGAC CCCTCCACCT 
AGACCTGAGG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT CACCTTCCCA 
GCTTCCAAAT CCCAGGACCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTACGAGGAG 
AAGCCACACA TGCACCCAGA CAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACCCGT 
TCCCAAGAGG AACTTCGGGA GGATAAAGCA TACCAGCTAG AGAGGCATCA AATCGAGGCA 
GTCTTGGATC GCAAGTCTGA TAGTGATATG TGGATCCATC AGAGCTCCTC AGTGGGCTCC 
AGCACCTCCA GCCAAGAGCA CCTGGACCAC TCCTCCAAGT CAGGCACCCC CGCGTCCACT 
CTGACCAAGA GCGGCCCTGG GCGCTGGAAG ACGCCAGCCG CTGTGCAGCC CATGCCCGCG 
GCCGTCTCCC AGCCTGTCCA AACAGATCCG CCCCCGCCAC CGCCACCGCC TCCCACACAC 
TATGCCGGAG ACTTTGACGG AATCCCCGTG GACTTGCCTC TCCCTCCGCC TCCTGCCGGC 
CAGACAGGAC CCGCGTCTGC TCAGGCGGCG GCCGCAGAGC GGCGGAAGCG GGAGGAGCAC 
CAGCGGTGGT ATGAGAAGGA GAAGGCCCGT CTAGAAGAGG AGCGGGACAG GAAGCGCAGG 
GAGCAGGAGC GGAAGCTGGG CCAGATGCGC TCCCAGTCCC TGAACCCGGC CCCGTTCTCT 
CCTGTGACCG TCCCACACGT GAAGCCAGAA AAACCGTCCA CACTGCAGAG GCCCCAGGAG 
ACGGTCATTC GGGAGCTGCA GCCCCAGCAG CAGCCCCGCA CGATTGAGCG CAGAGACTTA 
CAGTACATTA CGGTCAGCAA AGAGGAGCTG TCGTCGGGAG ACAGTCTGTC CCCAGACCCG 
TGGAAGCGGG ATGCCAGGGA GAAGCTGGAG AAGCAGCAGC AGATGCACAT CGTGGATATG 
CTGAGCAGGG AGATCCACGA GCTCCAGAGC AAGCCCGAGC GCAGTGCGGA GGAGAACGAC 
CGCCTGCGGA AGCTCATGCT GGAGTGGCAG TTCCAGAAGA GACTCCAGGA GTCCAAACAG 
AAGGACGAGG ATGACGAGGA AGAGGAGGAC GATGACGTGG ATACCATGCT GATCATGCAG 
CGCCTTGAGG CTGAGCGGAG AGCAAGGCAG ACTGCTCTGC CAGCAATCTC TGTTCTAGAT 
TTGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799121.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654680.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OAc92443
Clone ID Related Accession (Same CDS sequence)	XM_019799121.1
Accession Version	XM_019799121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGACACAG 
AAGAATATCG CAGGGGCGCT TGCCTTCTGG ATGGCCAATG CGTCTGAGCT CCTCAACTTT 
GTCAAACAGG ACCGAGATCT TAGTCGGATC ACACTGGATG CCCAGGACGT TTTAGCACAC 
TTGGTTCAGA TGGCATTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC 
ATGCCAGCCT TTCTGGATGA CCCTGAGGAG AACAGCCTAC AAAGACCAAA AATAGATGAT 
GTTCTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTCAACAGG 
CTGGTGACCG ACCCCGAGTC AGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGAC ACGTGGAAGC CTGGGCAGAG AAGCAGGGCT TGGAGCTGGC CGCCGACTGT 
CACCTCAGCC GGATAGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT 
GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAACT CACTGCAACT GCAAGCCTTG 
TTACAGAATT ACCACTGTGC ACCCGATGAG CCTTTTATCC CCACGGACCT GATAGAAAAT 
GTGGTGGCTG TGGCTGAAAA CACGGCTGAT GAGCTGGCGC GCAGTGACGG GAGGGAGGTG 
CAGCTCGAAG AGGACCCCGA TCTGCAGCTG CCTTTTCTTT TGCCAGAAGA TGGCTATTCT 
TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTGGACCC TTTGTGCCAG 
AGAGGATTTT GCAGGCTCGT CCCTCACGCA CGCTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCACCTTCTG CGCGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAAAAGC AAAATGGGAT GGGCCTCAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTCGGAA TCTATGTCAA GTCTGTTGTA 
AAAGGAGGTG CTGCGGATGT GGATGGACGC CTGGCTGCAG GTGACCAGCT CCTCAGTGTG 
GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGTTCTGTGG TAACACTGGA AGTAGCAAAG CAGGGAGCAA TTTATCACGG CCTCGCCACC 
CTGCTCAATC AGCCGTCCCC GATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCCAAA CGGGTCACCT 
GAGAGCCCTC AGCTGCCCTG GGCAGAATAC AGTGAACCGA AGAAATTGCC AGGCGACGAC 
AGGCTGATGA AGAACCGGGC TGATCACCGC TCTAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGCCCTGGAG GGAAGAGCAC GTACGCCCCC GGAACAACAG CTAAGATAAC GTCTGTCTCC 
ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACCCAGAC 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAAGAGGA ACTTCGGGAG 
GATAAAGCAT ACCAGCTAGA GAGGCATCAA ATCGAGGCAG TCTTGGATCG CAAGTCTGAT 
AGTGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCCA GCACCTCCAG CCAAGAGCAC 
CTGGACCACT CCTCCAAGTC AGGCACCCCC GCGTCCACTC TGACCAAGAG CGGCCCTGGG 
CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC ATGCCCGCGG CCGTCTCCCA GCCTGTCCAA 
ACAGATCCGC CCCCGCCACC GCCACCGCCT CCCACACACT ATGCCGGAGA CTTTGACGGA 
ATCCCCGTGG ACTTGCCTCT CCCTCCGCCT CCTGCCGGCC AGACAGGACC CGCGTCTGCT 
CAGGCGGCGG CCGCAGAGCG GCGGAAGCGG GAGGAGCACC AGCGGTGGTA TGAGAAGGAG 
AAGGCCCGTC TAGAAGAGGA GCGGGACAGG AAGCGCAGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGTCCCT GAACCCGGCC CCGTTCTCTC CTGTGACCGT CCCACACGTG 
AAGCCAGAAA AACCGTCCAC ACTGCAGAGG CCCCAGGAGA CGGTCATTCG GGAGCTGCAG 
CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC GGTCAGCAAA 
GAGGAGCTGT CGTCGGGAGA CAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCACGAG 
CTCCAGAGCA AGCCCGAGCG CAGTGCGGAG GAGAACGACC GCCTGCGGAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG TCCAAACAGA AGGACGAGGA TGACGAGGAA 
GAGGAGGACG ATGACGTGGA TACCATGCTG ATCATGCAGC GCCTTGAGGC TGAGCGGAGA 
GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGACCAA ACTCTTACAT GGGATCGGCT GGGACAGCTG TTGGTGCCCA TGATGCTTAT 
CGGGATCCGA GAGAGAGACT GTCTAAAAGC CACGATGCAG ATTTACCTGG GAGTCCGGGA 
GCCCCTGAGA ACTTGACGTT CAGAGAGCGC CAGCGCCTGT TTTCACAAGG TCAAGATGTG 
TCCAACAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654680.1
CDS	18..5360
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799121.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799121.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAGCCCCTA GTTGTTCAGC TGAATTGGAA TAAAGATGAT 
CGAGAGGGCA GATTTGTTCT TAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAGAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACCCTC 
TCAAAGAAAG AAAAGAAGGA GAAAAAGAAG AGAGAAAAAG AGGCAGTAAG GCAGGCGTCT 
GATAAGGATG ATAGGCCTTT CCAAGGGGAT GACGTTGAGA ATTCCCGACT GGCTGCCGAG 
GTTTACAAAG ACATGCCTGA AACTAGCTTT ACCCGAACAA TTTCGAATCC GGAGGTGGTC 
ATGAAGCGTC GCCGGCAGCA GAAACTAGAG AGGAGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCTG ACTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACCACA GACCCTGCAG AGTTTGCTGT GGCTGAAGCT 
TTAGAGAAAT ACGGCCTGGA GAAGGAGAAT CCCAAGGAGT ACTGCATTGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGACGAA AAAGGTGCTA AAGAAATTAT CCTTGATGAT 
GATGAGTGTC CTTTACAGAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGACCACC AGACTATATC CCAAAGAAAA CTAAGAAACA CATCGATGGG 
AAGCTGGTGA AGGGCAAGGA GAGAGCAGAG GGGTCGGGCT ATGGCTCCGC ACTTCCGCCC 
GAAAAGCTGC CCTATCTAGT AGAGCTGAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA CGGCTCTGAC TCCAGAGATA AGCCCAAGCT TTACCGCCTT 
CAGTTGAGCG TCACTGAGGT TGGGACAGAA AAGTTCGATG AGAACTCCAT CCAGCTGTTT 
GGATCAGGAA TTCAGCCCCA TCACTGTGAC CTCACAAACA TGGATGGGGT TGTCACTGTC 
ACGCCAAGAA GCATGGATGC TGAGACTTAT GTGGAAGGAC AGCGCATCTC AGAGACAACC 
ATGCTGCAAA GTGGAATGAA AGTGCAGTTC GGGGCATCAC ACGTGTTCAA GTTTGTGGAC 
CCCAGCCAGG ACCACGCTCT GGCAAAGAGA TCTGTGGATG GAGGCCTGAC AGCCAAGGGT 
CCCAGACATA AGCCTGGAAT TGTTCAAGAG ACAACTTTTG ATTTAGGAGG AGATGTTCAT 
AGTGGAACAG CACTGCCAAC AAGCAAGAGC ACCACGAGGC TGGACAGCGA CAGGGTGTTG 
TCTGCCTCCA GCACGGCGGA GCGCGGGATG GTGAAGCCAA TGATGAGAGT AGAACAGCAG 
CAGGATTACC GCAGGCAAGA AAACAGAACT CAGGACGCCC CTGGGCCTGA ACTGATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACCGT CCATTTTAAG TTGTCACCTA CATATGTATT GTATCTGGCA 
TGTCGGTATG TATTGTCCAG CCAGTCCAGA GCTGACGTCA GTCCTGCAGA GCGCACTCAC 
AAGGTGATTG CACTGGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGACACAG 
AAGAATATCG CAGGGGCGCT TGCCTTCTGG ATGGCCAATG CGTCTGAGCT CCTCAACTTT 
GTCAAACAGG ACCGAGATCT TAGTCGGATC ACACTGGATG CCCAGGACGT TTTAGCACAC 
TTGGTTCAGA TGGCATTTAA ATATCTGGTT CATTGTCTTC AATCAGAACT GAATAATTAC 
ATGCCAGCCT TTCTGGATGA CCCTGAGGAG AACAGCCTAC AAAGACCAAA AATAGATGAT 
GTTCTGCACA CGCTCACGGG AGCCATGTCT TTGCTGCGGC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC CCAGCTCTTC CACTTCGTCA ACATGTGGCT CTTCAACAGG 
CTGGTGACCG ACCCCGAGTC AGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGAC ACGTGGAAGC CTGGGCAGAG AAGCAGGGCT TGGAGCTGGC CGCCGACTGT 
CACCTCAGCC GGATAGTGCA GGCAACCACT TTGCTTACAA TGGATAAATA CGCACCTGAT 
GACATTCCAA ATATAAACAG TACCTGCTTT AAGTTAAACT CACTGCAACT GCAAGCCTTG 
TTACAGAATT ACCACTGTGC ACCCGATGAG CCTTTTATCC CCACGGACCT GATAGAAAAT 
GTGGTGGCTG TGGCTGAAAA CACGGCTGAT GAGCTGGCGC GCAGTGACGG GAGGGAGGTG 
CAGCTCGAAG AGGACCCCGA TCTGCAGCTG CCTTTTCTTT TGCCAGAAGA TGGCTATTCT 
TGTGATGTTG TCAGGAACAT TCCAAATGGT TTACAAGAAT TTTTGGACCC TTTGTGCCAG 
AGAGGATTTT GCAGGCTCGT CCCTCACGCA CGCTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCACCTTCTG CGCGAGAGCA CGGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACT CTTAAAAAGC AAAATGGGAT GGGCCTCAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTCGGAA TCTATGTCAA GTCTGTTGTA 
AAAGGAGGTG CTGCGGATGT GGATGGACGC CTGGCTGCAG GTGACCAGCT CCTCAGTGTG 
GACGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCGG CAGAACTCAT GACAAGAACA 
AGTTCTGTGG TAACACTGGA AGTAGCAAAG CAGGGAGCAA TTTATCACGG CCTCGCCACC 
CTGCTCAATC AGCCGTCCCC GATGATGCAA AGAATTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG TTTCGAGCTC TATAATAATT CAGCTCCAAA CGGGTCACCT 
GAGAGCCCTC AGCTGCCCTG GGCAGAATAC AGTGAACCGA AGAAATTGCC AGGCGACGAC 
AGGCTGATGA AGAACCGGGC TGATCACCGC TCTAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGCCCTGGAG GGAAGAGCAC GTACGCCCCC GGAACAACAG CTAAGATAAC GTCTGTCTCC 
ACTGGGAACC TCTGCACTGA GGAACAGACC CCTCCACCTA GACCTGAGGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTC ACCTTCCCAG CTTCCAAATC CCAGGACCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TACGAGGAGA AGCCACACAT GCACCCAGAC 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACCCGTT CCCAAGAGGA ACTTCGGGAG 
GATAAAGCAT ACCAGCTAGA GAGGCATCAA ATCGAGGCAG TCTTGGATCG CAAGTCTGAT 
AGTGATATGT GGATCCATCA GAGCTCCTCA GTGGGCTCCA GCACCTCCAG CCAAGAGCAC 
CTGGACCACT CCTCCAAGTC AGGCACCCCC GCGTCCACTC TGACCAAGAG CGGCCCTGGG 
CGCTGGAAGA CGCCAGCCGC TGTGCAGCCC ATGCCCGCGG CCGTCTCCCA GCCTGTCCAA 
ACAGATCCGC CCCCGCCACC GCCACCGCCT CCCACACACT ATGCCGGAGA CTTTGACGGA 
ATCCCCGTGG ACTTGCCTCT CCCTCCGCCT CCTGCCGGCC AGACAGGACC CGCGTCTGCT 
CAGGCGGCGG CCGCAGAGCG GCGGAAGCGG GAGGAGCACC AGCGGTGGTA TGAGAAGGAG 
AAGGCCCGTC TAGAAGAGGA GCGGGACAGG AAGCGCAGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGTCCCT GAACCCGGCC CCGTTCTCTC CTGTGACCGT CCCACACGTG 
AAGCCAGAAA AACCGTCCAC ACTGCAGAGG CCCCAGGAGA CGGTCATTCG GGAGCTGCAG 
CCCCAGCAGC AGCCCCGCAC GATTGAGCGC AGAGACTTAC AGTACATTAC GGTCAGCAAA 
GAGGAGCTGT CGTCGGGAGA CAGTCTGTCC CCAGACCCGT GGAAGCGGGA TGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA GATGCACATC GTGGATATGC TGAGCAGGGA GATCCACGAG 
CTCCAGAGCA AGCCCGAGCG CAGTGCGGAG GAGAACGACC GCCTGCGGAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG TCCAAACAGA AGGACGAGGA TGACGAGGAA 
GAGGAGGACG ATGACGTGGA TACCATGCTG ATCATGCAGC GCCTTGAGGC TGAGCGGAGA 
GCAAGGGATG AGGAGCGGAG GCGCCAGCAG CAGCTGGAAG AGATGCGCAA GCGGGAAGCC 
GAAGATCGCG CCAGGCAGGA GGAGGAGCGG CGCCGCCAGG AGGAGGAGAG AACAAAGCGA 
GATGCTGAAG AAAAGAGGCG ACAAGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG TTGCTGGAGC CGGAGGAGCC GGGTCTGTGC 
AGACCTCCGC TTCCGCGGGG CTACGAGCTC CCGTCCCTGT CCCCTCCGCC TGCCGCTCCT 
CCTCCCCCGC CTCAGCGGAC CGCCTCCTCC CTCCAGGCGC AGGCCCTCTC CCCAGACTCG 
GTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGCGG CGGAGGAGCA CTGCGGCCTG 
GCAGGACCAA ACTCTTACAT GGGATCGGCT GGGACAGCTG TTGGTGCCCA TGATGCTTAT 
CGGGATCCGA GAGAGAGACT GTCTAAAAGC CACGATGCAG ATTTACCTGG GAGTCCGGGA 
GCCCCTGAGA ACTTGACGTT CAGAGAGCGC CAGCGCCTGT TTTCACAAGG TCAAGATGTG 
TCCAACAAAG TAAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACAAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799122.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654681.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OAc92444
Clone ID Related Accession (Same CDS sequence)	XM_019799122.1
Accession Version	XM_019799122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAGA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC CCTCTCAAAG 
AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TAAGGCAGGC GTCTGATAAG 
GATGATAGGC CTTTCCAAGG GGATGACGTT GAGAATTCCC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CTGAAACTAG CTTTACCCGA ACAATTTCGA ATCCGGAGGT GGTCATGAAG 
CGTCGCCGGC AGCAGAAACT AGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCTGACTCAG GTGGAACATT GAGAATTTAT GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCTAC CACAGACCCT GCAGAGTTTG CTGTGGCTGA AGCTTTAGAG 
AAATACGGCC TGGAGAAGGA GAATCCCAAG GAGTACTGCA TTGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCTGA CGAAAAAGGT GCTAAAGAAA TTATCCTTGA TGATGATGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCA AGTGACAAAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGAC CACCAGACTA TATCCCAAAG AAAACTAAGA AACACATCGA TGGGAAGCTG 
GTGAAGGGCA AGGAGAGAGC AGAGGGGTCG GGCTATGGCT CCGCACTTCC GCCCGAAAAG 
CTGCCCTATC TAGTAGAGCT GAGCCCAGAC GGCTCTGACT CCAGAGATAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT CACTGAGGTT GGGACAGAAA AGTTCGATGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGGGTT 
GTCACTGTCA CGCCAAGAAG CATGGATGCT GAGACTTATG TGGAAGGACA GCGCATCTCA 
GAGACAACCA TGCTGCAAAG TGGAATGAAA GTGCAGTTCG GGGCATCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCTCTG GCAAAGAGAT CTGTGGATGG AGGCCTGACA 
GCCAAGGGTC CCAGACATAA GCCTGGAATT GTTCAAGAGA CAACTTTTGA TTTAGGAGGA 
GATGTTCATA GTGGAACAGC ACTGCCAACA AGCAAGAGCA CCACGAGGCT GGACAGCGAC 
AGGGTGTTGT CTGCCTCCAG CACGGCGGAG CGCGGGATGG TGAAGCCAAT GATGAGAGTA 
GAACAGCAGC AGGATTACCG CAGGCAAGAA AACAGAACTC AGGACGCCCC TGGGCCTGAA 
CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACCGTC CATTTTAAGT TGTCACCTAC ATATGTATTG 
TATCTGGCAT GTCGGTATGT ATTGTCCAGC CAGTCCAGAG CTGACGTCAG TCCTGCAGAG 
CGCACTCACA AGGTGATTGC ACTGGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGACACAGA AGAATATCGC AGGGGCGCTT GCCTTCTGGA TGGCCAATGC GTCTGAGCTC 
CTCAACTTTG TCAAACAGGA CCGAGATCTT AGTCGGATCA CACTGGATGC CCAGGACGTT 
TTAGCACACT TGGTTCAGAT GGCATTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG 
AATAATTACA TGCCAGCCTT TCTGGATGAC CCTGAGGAGA ACAGCCTACA AAGACCAAAA 
ATAGATGATG TTCTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC 
AATGCCGCCC TGACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTCAACAGGC TGGTGACCGA CCCCGAGTCA GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGACA CGTGGAAGCC TGGGCAGAGA AGCAGGGCTT GGAGCTGGCC 
GCCGACTGTC ACCTCAGCCG GATAGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC 
GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAACTC ACTGCAACTG 
CAAGCCTTGT TACAGAATTA CCACTGTGCA CCCGATGAGC CTTTTATCCC CACGGACCTG 
ATAGAAAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGATG AGCTGGCGCG CAGTGACGGG 
AGGGAGGTGC AGCTCGAAGA GGACCCCGAT CTGCAGCTGC CTTTTCTTTT GCCAGAAGAT 
GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTGGACCCT 
TTGTGCCAGA GAGGATTTTG CAGGCTCGTC CCTCACGCAC GCTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CACCTTCTGC GCGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAAAAGCA AAATGGGATG 
GGCCTCAGCA TTGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTCGGAAT CTATGTCAAG 
TCTGTTGTAA AAGGAGGTGC TGCGGATGTG GATGGACGCC TGGCTGCAGG TGACCAGCTC 
CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAACTCATG 
ACAAGAACAA GTTCTGTGGT AACACTGGAA GTAGCAAAGC AGGGAGCAAT TTATCACGGC 
CTCGCCACCC TGCTCAATCA GCCGTCCCCG ATGATGCAAA GAATTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCCAAAC 
GGGTCACCTG AGAGCCCTCA GCTGCCCTGG GCAGAATACA GTGAACCGAA GAAATTGCCA 
GGCGACGACA GGCTGATGAA GAACCGGGCT GATCACCGCT CTAGCCCCAA CGTAGCAAAT 
CAGCCTCCTA GCCCTGGAGG GAAGAGCACG TACGCCCCCG GAACAACAGC TAAGATAACG 
TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCC 
TACCCCATCC CCACTCAGAC GTACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC 
CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG 
CACCCAGACA GTAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAAGAGGAA 
CTTCGGGAGG ATAAAGCATA CCAGCTAGAG AGGCATCAAA TCGAGGCAGT CTTGGATCGC 
AAGTCTGATA GTGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCCAG CACCTCCAGC 
CAAGAGCACC TGGACCACTC CTCCAAGTCA GGCACCCCCG CGTCCACTCT GACCAAGAGC 
GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCA TGCCCGCGGC CGTCTCCCAG 
CCTGTCCAAA CAGATCCGCC CCCGCCACCG CCACCGCCTC CCACACACTA TGCCGGAGAC 
TTTGACGGAA TCCCCGTGGA CTTGCCTCTC CCTCCGCCTC CTGCCGGCCA GACAGGACCC 
GCGTCTGCTC AGGCGGCGGC CGCAGAGCGG CGGAAGCGGG AGGAGCACCA GCGGTGGTAT 
GAGAAGGAGA AGGCCCGTCT AGAAGAGGAG CGGGACAGGA AGCGCAGGGA GCAGGAGCGG 
AAGCTGGGCC AGATGCGCTC CCAGTCCCTG AACCCGGCCC CGTTCTCTCC TGTGACCGTC 
CCACACGTGA AGCCAGAAAA ACCGTCCACA CTGCAGAGGC CCCAGGAGAC GGTCATTCGG 
GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACG 
GTCAGCAAAG AGGAGCTGTC GTCGGGAGAC AGTCTGTCCC CAGACCCGTG GAAGCGGGAT 
GCCAGGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG 
ATCCACGAGC TCCAGAGCAA GCCCGAGCGC AGTGCGGAGG AGAACGACCG CCTGCGGAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CCAAACAGAA GGACGAGGAT 
GACGAGGAAG AGGAGGACGA TGACGTGGAT ACCATGCTGA TCATGCAGCG CCTTGAGGCT 
GAGCGGAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT 
GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGGGAAGC CGAAGATCGC 
GCCAGGCAGG AGGAGGAGCG GCGCCGCCAG GAGGAGGAGA GAACAAAGCG AGATGCTGAA 
GAAAAGAGGC GACAAGAAGA AGGTTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG 
CTTCCGCGGG GCTACGAGCT CCCGTCCCTG TCCCCTCCGC CTGCCGCTCC TCCTCCCCCG 
CCTCAGCGGA CCGCCTCCTC CCTCCAGGCG CAGGCCCTCT CCCCAGACTC GGTGTACACC 
GCCAGGCTCG TTGCGTACCA CGAGGAGGCG GCGGAGGAGC ACTGCGGCCT GGCAGGTCAG 
GATAAGCACT CTTGCACGAG GAAGTCCCTC GGGCACCTTC TTCTTCCCGC CGCCCTTAAG 
CCGCAGCAGC CCCCCTGCCC GCCGCGCCCG GCCTCCGATG GCATCTTTCT CTCCAACTCA 
TTCCAGCCAC CCTCGGCCCG TGCCGACAGT ACTGCTCACA AAGCAGGCCA GCCCCCGCCC 
CCTCCAAAAA AACCAGGCTT CTGCCAGCCC AGCCACTCGA AAGGACCAAA CTCTTACATG 
GGATCGGCTG GGACAGCTGT TGGTGCCCAT GATGCTTATC GGGATCCGAG AGAGAGACTG 
TCTAAAAGCC ACGATGCAGA TTTACCTGGG AGTCCGGGAG CCCCTGAGAA CTTGACGTTC 
AGAGAGCGCC AGCGCCTGTT TTCACAAGGT CAAGATGTGT CCAACAAAGT AAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654681.1
CDS	18..5579
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799122.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799122.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAGA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC CCTCTCAAAG 
AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TAAGGCAGGC GTCTGATAAG 
GATGATAGGC CTTTCCAAGG GGATGACGTT GAGAATTCCC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CTGAAACTAG CTTTACCCGA ACAATTTCGA ATCCGGAGGT GGTCATGAAG 
CGTCGCCGGC AGCAGAAACT AGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCTGACTCAG GTGGAACATT GAGAATTTAT GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCTAC CACAGACCCT GCAGAGTTTG CTGTGGCTGA AGCTTTAGAG 
AAATACGGCC TGGAGAAGGA GAATCCCAAG GAGTACTGCA TTGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCTGA CGAAAAAGGT GCTAAAGAAA TTATCCTTGA TGATGATGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCA AGTGACAAAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGAC CACCAGACTA TATCCCAAAG AAAACTAAGA AACACATCGA TGGGAAGCTG 
GTGAAGGGCA AGGAGAGAGC AGAGGGGTCG GGCTATGGCT CCGCACTTCC GCCCGAAAAG 
CTGCCCTATC TAGTAGAGCT GAGCCCAGAC GGCTCTGACT CCAGAGATAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT CACTGAGGTT GGGACAGAAA AGTTCGATGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGGGTT 
GTCACTGTCA CGCCAAGAAG CATGGATGCT GAGACTTATG TGGAAGGACA GCGCATCTCA 
GAGACAACCA TGCTGCAAAG TGGAATGAAA GTGCAGTTCG GGGCATCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCTCTG GCAAAGAGAT CTGTGGATGG AGGCCTGACA 
GCCAAGGGTC CCAGACATAA GCCTGGAATT GTTCAAGAGA CAACTTTTGA TTTAGGAGGA 
GATGTTCATA GTGGAACAGC ACTGCCAACA AGCAAGAGCA CCACGAGGCT GGACAGCGAC 
AGGGTGTTGT CTGCCTCCAG CACGGCGGAG CGCGGGATGG TGAAGCCAAT GATGAGAGTA 
GAACAGCAGC AGGATTACCG CAGGCAAGAA AACAGAACTC AGGACGCCCC TGGGCCTGAA 
CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACCGTC CATTTTAAGT TGTCACCTAC ATATGTATTG 
TATCTGGCAT GTCGGTATGT ATTGTCCAGC CAGTCCAGAG CTGACGTCAG TCCTGCAGAG 
CGCACTCACA AGGTGATTGC ACTGGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGACACAGA AGAATATCGC AGGGGCGCTT GCCTTCTGGA TGGCCAATGC GTCTGAGCTC 
CTCAACTTTG TCAAACAGGA CCGAGATCTT AGTCGGATCA CACTGGATGC CCAGGACGTT 
TTAGCACACT TGGTTCAGAT GGCATTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG 
AATAATTACA TGCCAGCCTT TCTGGATGAC CCTGAGGAGA ACAGCCTACA AAGACCAAAA 
ATAGATGATG TTCTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC 
AATGCCGCCC TGACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTCAACAGGC TGGTGACCGA CCCCGAGTCA GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGACA CGTGGAAGCC TGGGCAGAGA AGCAGGGCTT GGAGCTGGCC 
GCCGACTGTC ACCTCAGCCG GATAGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC 
GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAACTC ACTGCAACTG 
CAAGCCTTGT TACAGAATTA CCACTGTGCA CCCGATGAGC CTTTTATCCC CACGGACCTG 
ATAGAAAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGATG AGCTGGCGCG CAGTGACGGG 
AGGGAGGTGC AGCTCGAAGA GGACCCCGAT CTGCAGCTGC CTTTTCTTTT GCCAGAAGAT 
GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTGGACCCT 
TTGTGCCAGA GAGGATTTTG CAGGCTCGTC CCTCACGCAC GCTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CACCTTCTGC GCGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAAAAGCA AAATGGGATG 
GGCCTCAGCA TTGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTCGGAAT CTATGTCAAG 
TCTGTTGTAA AAGGAGGTGC TGCGGATGTG GATGGACGCC TGGCTGCAGG TGACCAGCTC 
CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAACTCATG 
ACAAGAACAA GTTCTGTGGT AACACTGGAA GTAGCAAAGC AGGGAGCAAT TTATCACGGC 
CTCGCCACCC TGCTCAATCA GCCGTCCCCG ATGATGCAAA GAATTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCCAAAC 
GGGTCACCTG AGAGCCCTCA GCTGCCCTGG GCAGAATACA GTGAACCGAA GAAATTGCCA 
GGCGACGACA GGCTGATGAA GAACCGGGCT GATCACCGCT CTAGCCCCAA CGTAGCAAAT 
CAGCCTCCTA GCCCTGGAGG GAAGAGCACG TACGCCCCCG GAACAACAGC TAAGATAACG 
TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCC 
TACCCCATCC CCACTCAGAC GTACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC 
CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG 
CACCCAGACA GTAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAAGAGGAA 
CTTCGGGAGG ATAAAGCATA CCAGCTAGAG AGGCATCAAA TCGAGGCAGT CTTGGATCGC 
AAGTCTGATA GTGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCCAG CACCTCCAGC 
CAAGAGCACC TGGACCACTC CTCCAAGTCA GGCACCCCCG CGTCCACTCT GACCAAGAGC 
GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCA TGCCCGCGGC CGTCTCCCAG 
CCTGTCCAAA CAGATCCGCC CCCGCCACCG CCACCGCCTC CCACACACTA TGCCGGAGAC 
TTTGACGGAA TCCCCGTGGA CTTGCCTCTC CCTCCGCCTC CTGCCGGCCA GACAGGACCC 
GCGTCTGCTC AGGCGGCGGC CGCAGAGCGG CGGAAGCGGG AGGAGCACCA GCGGTGGTAT 
GAGAAGGAGA AGGCCCGTCT AGAAGAGGAG CGGGACAGGA AGCGCAGGGA GCAGGAGCGG 
AAGCTGGGCC AGATGCGCTC CCAGTCCCTG AACCCGGCCC CGTTCTCTCC TGTGACCGTC 
CCACACGTGA AGCCAGAAAA ACCGTCCACA CTGCAGAGGC CCCAGGAGAC GGTCATTCGG 
GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACG 
GTCAGCAAAG AGGAGCTGTC GTCGGGAGAC AGTCTGTCCC CAGACCCGTG GAAGCGGGAT 
GCCAGGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG 
ATCCACGAGC TCCAGAGCAA GCCCGAGCGC AGTGCGGAGG AGAACGACCG CCTGCGGAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CCAAACAGAA GGACGAGGAT 
GACGAGGAAG AGGAGGACGA TGACGTGGAT ACCATGCTGA TCATGCAGCG CCTTGAGGCT 
GAGCGGAGAG CAAGGCAGAC TGCTCTGCCA GCAATCTCTG TTCTAGATTT GTTACAGGAT 
GAGGAGCGGA GGCGCCAGCA GCAGCTGGAA GAGATGCGCA AGCGGGAAGC CGAAGATCGC 
GCCAGGCAGG AGGAGGAGCG GCGCCGCCAG GAGGAGGAGA GAACAAAGCG AGATGCTGAA 
GAAAAGAGGC GACAAGAAGA AGGTTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGCGGAGG CCGAGCGCAG GTTGCTGGAG CCGGAGGAGC CGGGTCTGTG CAGACCTCCG 
CTTCCGCGGG GCTACGAGCT CCCGTCCCTG TCCCCTCCGC CTGCCGCTCC TCCTCCCCCG 
CCTCAGCGGA CCGCCTCCTC CCTCCAGGCG CAGGCCCTCT CCCCAGACTC GGTGTACACC 
GCCAGGCTCG TTGCGTACCA CGAGGAGGCG GCGGAGGAGC ACTGCGGCCT GGCAGGTCAG 
GATAAGCACT CTTGCACGAG GAAGTCCCTC GGGCACCTTC TTCTTCCCGC CGCCCTTAAG 
CCGCAGCAGC CCCCCTGCCC GCCGCGCCCG GCCTCCGATG GCATCTTTCT CTCCAACTCA 
TTCCAGCCAC CCTCGGCCCG TGCCGACAGT ACTGCTCACA AAGCAGGCCA GCCCCCGCCC 
CCTCCAAAAA AACCAGGCTT CTGCCAGCCC AGCCACTCGA AAGGACCAAA CTCTTACATG 
GGATCGGCTG GGACAGCTGT TGGTGCCCAT GATGCTTATC GGGATCCGAG AGAGAGACTG 
TCTAAAAGCC ACGATGCAGA TTTACCTGGG AGTCCGGGAG CCCCTGAGAA CTTGACGTTC 
AGAGAGCGCC AGCGCCTGTT TTCACAAGGT CAAGATGTGT CCAACAAAGT AAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799123.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654682.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OAc92445
Clone ID Related Accession (Same CDS sequence)	XM_019799123.1
Accession Version	XM_019799123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGTTACAG GATGAGGAGC GGAGGCGCCA GCAGCAGCTG 
GAAGAGATGC GCAAGCGGGA AGCCGAAGAT CGCGCCAGGC AGGAGGAGGA GCGGCGCCGC 
CAGGAGGAGG AGAGAACAAA GCGAGATGCT GAAGAAAAGA GGCGACAAGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGTTGCTG 
GAGCCGGAGG AGCCGGGTCT GTGCAGACCT CCGCTTCCGC GGGGCTACGA GCTCCCGTCC 
CTGTCCCCTC CGCCTGCCGC TCCTCCTCCC CCGCCTCAGC GGACCGCCTC CTCCCTCCAG 
GCGCAGGCCC TCTCCCCAGA CTCGGTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GCGGCGGAGG AGCACTGCGG CCTGGCAGGA CCAAACTCTT ACATGGGATC GGCTGGGACA 
GCTGTTGGTG CCCATGATGC TTATCGGGAT CCGAGAGAGA GACTGTCTAA AAGCCACGAT 
GCAGATTTAC CTGGGAGTCC GGGAGCCCCT GAGAACTTGA CGTTCAGAGA GCGCCAGCGC 
CTGTTTTCAC AAGGTCAAGA TGTGTCCAAC AAAGTAAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654682.1
CDS	18..5384
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQEVDQVDQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799123.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799123.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GGAAGTAGAC 
CAGGTTGATC AGACACAGAA GAATATCGCA GGGGCGCTTG CCTTCTGGAT GGCCAATGCG 
TCTGAGCTCC TCAACTTTGT CAAACAGGAC CGAGATCTTA GTCGGATCAC ACTGGATGCC 
CAGGACGTTT TAGCACACTT GGTTCAGATG GCATTTAAAT ATCTGGTTCA TTGTCTTCAA 
TCAGAACTGA ATAATTACAT GCCAGCCTTT CTGGATGACC CTGAGGAGAA CAGCCTACAA 
AGACCAAAAA TAGATGATGT TCTGCACACG CTCACGGGAG CCATGTCTTT GCTGCGGCGC 
TGCAGAGTCA ATGCCGCCCT GACCATCCAG CTCTTCTCCC AGCTCTTCCA CTTCGTCAAC 
ATGTGGCTCT TCAACAGGCT GGTGACCGAC CCCGAGTCAG GACTGTGCTC TCACTACTGG 
GGGGCAATCA TCCGCCAGCA GCTGGGACAC GTGGAAGCCT GGGCAGAGAA GCAGGGCTTG 
GAGCTGGCCG CCGACTGTCA CCTCAGCCGG ATAGTGCAGG CAACCACTTT GCTTACAATG 
GATAAATACG CACCTGATGA CATTCCAAAT ATAAACAGTA CCTGCTTTAA GTTAAACTCA 
CTGCAACTGC AAGCCTTGTT ACAGAATTAC CACTGTGCAC CCGATGAGCC TTTTATCCCC 
ACGGACCTGA TAGAAAATGT GGTGGCTGTG GCTGAAAACA CGGCTGATGA GCTGGCGCGC 
AGTGACGGGA GGGAGGTGCA GCTCGAAGAG GACCCCGATC TGCAGCTGCC TTTTCTTTTG 
CCAGAAGATG GCTATTCTTG TGATGTTGTC AGGAACATTC CAAATGGTTT ACAAGAATTT 
TTGGACCCTT TGTGCCAGAG AGGATTTTGC AGGCTCGTCC CTCACGCACG CTCACCAGGT 
ACTTGGACGA TATATTTTGA AGGTGCAGAT TATGAGAGCC ACCTTCTGCG CGAGAGCACG 
GAGCTGGCTC AGCCTCTGAG GAAAGAACCT GAAATAATCA CTGTGACTCT TAAAAAGCAA 
AATGGGATGG GCCTCAGCAT TGTTGCAGCA AAGGGTGCTG GTCAAGATAA ACTCGGAATC 
TATGTCAAGT CTGTTGTAAA AGGAGGTGCT GCGGATGTGG ATGGACGCCT GGCTGCAGGT 
GACCAGCTCC TCAGTGTGGA CGGACGAAGT CTGGTAGGAC TCTCTCAAGA AAGGGCGGCA 
GAACTCATGA CAAGAACAAG TTCTGTGGTA ACACTGGAAG TAGCAAAGCA GGGAGCAATT 
TATCACGGCC TCGCCACCCT GCTCAATCAG CCGTCCCCGA TGATGCAAAG AATTTCAGAC 
CGTCGTGGCT CAGGTAAACC CCGACCAAAG AGTGAAGGTT TCGAGCTCTA TAATAATTCA 
GCTCCAAACG GGTCACCTGA GAGCCCTCAG CTGCCCTGGG CAGAATACAG TGAACCGAAG 
AAATTGCCAG GCGACGACAG GCTGATGAAG AACCGGGCTG ATCACCGCTC TAGCCCCAAC 
GTAGCAAATC AGCCTCCTAG CCCTGGAGGG AAGAGCACGT ACGCCCCCGG AACAACAGCT 
AAGATAACGT CTGTCTCCAC TGGGAACCTC TGCACTGAGG AACAGACCCC TCCACCTAGA 
CCTGAGGCCT ACCCCATCCC CACTCAGACG TACACCAGAG AGTATTTCAC CTTCCCAGCT 
TCCAAATCCC AGGACCGGAT GGCTCCTCCT CAGAACCAGT GGCCAAATTA CGAGGAGAAG 
CCACACATGC ACCCAGACAG TAATCATTCC AGTATTGCAA TTCAGCGTGT TACCCGTTCC 
CAAGAGGAAC TTCGGGAGGA TAAAGCATAC CAGCTAGAGA GGCATCAAAT CGAGGCAGTC 
TTGGATCGCA AGTCTGATAG TGATATGTGG ATCCATCAGA GCTCCTCAGT GGGCTCCAGC 
ACCTCCAGCC AAGAGCACCT GGACCACTCC TCCAAGTCAG GCACCCCCGC GTCCACTCTG 
ACCAAGAGCG GCCCTGGGCG CTGGAAGACG CCAGCCGCTG TGCAGCCCAT GCCCGCGGCC 
GTCTCCCAGC CTGTCCAAAC AGATCCGCCC CCGCCACCGC CACCGCCTCC CACACACTAT 
GCCGGAGACT TTGACGGAAT CCCCGTGGAC TTGCCTCTCC CTCCGCCTCC TGCCGGCCAG 
ACAGGACCCG CGTCTGCTCA GGCGGCGGCC GCAGAGCGGC GGAAGCGGGA GGAGCACCAG 
CGGTGGTATG AGAAGGAGAA GGCCCGTCTA GAAGAGGAGC GGGACAGGAA GCGCAGGGAG 
CAGGAGCGGA AGCTGGGCCA GATGCGCTCC CAGTCCCTGA ACCCGGCCCC GTTCTCTCCT 
GTGACCGTCC CACACGTGAA GCCAGAAAAA CCGTCCACAC TGCAGAGGCC CCAGGAGACG 
GTCATTCGGG AGCTGCAGCC CCAGCAGCAG CCCCGCACGA TTGAGCGCAG AGACTTACAG 
TACATTACGG TCAGCAAAGA GGAGCTGTCG TCGGGAGACA GTCTGTCCCC AGACCCGTGG 
AAGCGGGATG CCAGGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGATATGCTG 
AGCAGGGAGA TCCACGAGCT CCAGAGCAAG CCCGAGCGCA GTGCGGAGGA GAACGACCGC 
CTGCGGAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC CAAACAGAAG 
GACGAGGATG ACGAGGAAGA GGAGGACGAT GACGTGGATA CCATGCTGAT CATGCAGCGC 
CTTGAGGCTG AGCGGAGAGC AAGGTTACAG GATGAGGAGC GGAGGCGCCA GCAGCAGCTG 
GAAGAGATGC GCAAGCGGGA AGCCGAAGAT CGCGCCAGGC AGGAGGAGGA GCGGCGCCGC 
CAGGAGGAGG AGAGAACAAA GCGAGATGCT GAAGAAAAGA GGCGACAAGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGTTGCTG 
GAGCCGGAGG AGCCGGGTCT GTGCAGACCT CCGCTTCCGC GGGGCTACGA GCTCCCGTCC 
CTGTCCCCTC CGCCTGCCGC TCCTCCTCCC CCGCCTCAGC GGACCGCCTC CTCCCTCCAG 
GCGCAGGCCC TCTCCCCAGA CTCGGTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GCGGCGGAGG AGCACTGCGG CCTGGCAGGA CCAAACTCTT ACATGGGATC GGCTGGGACA 
GCTGTTGGTG CCCATGATGC TTATCGGGAT CCGAGAGAGA GACTGTCTAA AAGCCACGAT 
GCAGATTTAC CTGGGAGTCC GGGAGCCCCT GAGAACTTGA CGTTCAGAGA GCGCCAGCGC 
CTGTTTTCAC AAGGTCAAGA TGTGTCCAAC AAAGTAAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799124.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654683.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OAc92446
Clone ID Related Accession (Same CDS sequence)	XM_019799124.1
Accession Version	XM_019799124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654683.1
CDS	18..5363
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799124.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799124.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ATGAGGAGCG GAGGCGCCAG CAGCAGCTGG AAGAGATGCG CAAGCGGGAA 
GCCGAAGATC GCGCCAGGCA GGAGGAGGAG CGGCGCCGCC AGGAGGAGGA GAGAACAAAG 
CGAGATGCTG AAGAAAAGAG GCGACAAGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGTTGCTGG AGCCGGAGGA GCCGGGTCTG 
TGCAGACCTC CGCTTCCGCG GGGCTACGAG CTCCCGTCCC TGTCCCCTCC GCCTGCCGCT 
CCTCCTCCCC CGCCTCAGCG GACCGCCTCC TCCCTCCAGG CGCAGGCCCT CTCCCCAGAC 
TCGGTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG CGGCGGAGGA GCACTGCGGC 
CTGGCAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799125.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654684.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OAc92447
Clone ID Related Accession (Same CDS sequence)	XM_019799125.1
Accession Version	XM_019799125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGCAGACTG CTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGATGA GGAGCGGAGG 
CGCCAGCAGC AGCTGGAAGA GATGCGCAAG CGGGAAGCCG AAGATCGCGC CAGGCAGGAG 
GAGGAGCGGC GCCGCCAGGA GGAGGAGAGA ACAAAGCGAG ATGCTGAAGA AAAGGTTATG 
GTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654684.1
CDS	18..4886
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTALPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	XM_019799125.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799125.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGCAGACTG CTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGATGA GGAGCGGAGG 
CGCCAGCAGC AGCTGGAAGA GATGCGCAAG CGGGAAGCCG AAGATCGCGC CAGGCAGGAG 
GAGGAGCGGC GCCGCCAGGA GGAGGAGAGA ACAAAGCGAG ATGCTGAAGA AAAGGTTATG 
GTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799126.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654685.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OAc92448
Clone ID Related Accession (Same CDS sequence)	XM_019799126.1
Accession Version	XM_019799126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA 
GATCGCGCCA GGCAGGAGGA GGAGCGGCGC CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT 
GCTGAAGAAA AGGTTATGGT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654685.1
CDS	18..4844
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	XM_019799126.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799126.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AGAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACCCTCTCA 
AAGAAAGAAA AGAAGGAGAA AAAGAAGAGA GAAAAAGAGG CAGTAAGGCA GGCGTCTGAT 
AAGGATGATA GGCCTTTCCA AGGGGATGAC GTTGAGAATT CCCGACTGGC TGCCGAGGTT 
TACAAAGACA TGCCTGAAAC TAGCTTTACC CGAACAATTT CGAATCCGGA GGTGGTCATG 
AAGCGTCGCC GGCAGCAGAA ACTAGAGAGG AGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCTGACT CAGGTGGAAC ATTGAGAATT TATGCAGATA GTTTAAAACC AAATATTCCC 
TACAAGACAA TCCTGCTGTC TACCACAGAC CCTGCAGAGT TTGCTGTGGC TGAAGCTTTA 
GAGAAATACG GCCTGGAGAA GGAGAATCCC AAGGAGTACT GCATTGCCCG GGTTATGCTT 
CCTCCTGGAG CCCAGCATTC TGACGAAAAA GGTGCTAAAG AAATTATCCT TGATGATGAT 
GAGTGTCCTT TACAGATCTT CAGGGAATGG CCAAGTGACA AAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GACCACCAGA CTATATCCCA AAGAAAACTA AGAAACACAT CGATGGGAAG 
CTGGTGAAGG GCAAGGAGAG AGCAGAGGGG TCGGGCTATG GCTCCGCACT TCCGCCCGAA 
AAGCTGCCCT ATCTAGTAGA GCTGAGCCCA GGGAGAAGGA ATCACTTTGC CTACTACAAC 
TATCACACTT ACGAAGACGG CTCTGACTCC AGAGATAAGC CCAAGCTTTA CCGCCTTCAG 
TTGAGCGTCA CTGAGGTTGG GACAGAAAAG TTCGATGAGA ACTCCATCCA GCTGTTTGGA 
TCAGGAATTC AGCCCCATCA CTGTGACCTC ACAAACATGG ATGGGGTTGT CACTGTCACG 
CCAAGAAGCA TGGATGCTGA GACTTATGTG GAAGGACAGC GCATCTCAGA GACAACCATG 
CTGCAAAGTG GAATGAAAGT GCAGTTCGGG GCATCACACG TGTTCAAGTT TGTGGACCCC 
AGCCAGGACC ACGCTCTGGC AAAGAGATCT GTGGATGGAG GCCTGACAGC CAAGGGTCCC 
AGACATAAGC CTGGAATTGT TCAAGAGACA ACTTTTGATT TAGGAGGAGA TGTTCATAGT 
GGAACAGCAC TGCCAACAAG CAAGAGCACC ACGAGGCTGG ACAGCGACAG GGTGTTGTCT 
GCCTCCAGCA CGGCGGAGCG CGGGATGGTG AAGCCAATGA TGAGAGTAGA ACAGCAGCAG 
GATTACCGCA GGCAAGAAAA CAGAACTCAG GACGCCCCTG GGCCTGAACT GATACTGCCT 
GCAAGCATTG AATTCAGGGA AAGTTCTGAA GATTCATTTT TATCTGCCAT TATAAATTAT 
ACTAATAGCT CTACCGTCCA TTTTAAGTTG TCACCTACAT ATGTATTGTA TCTGGCATGT 
CGGTATGTAT TGTCCAGCCA GTCCAGAGCT GACGTCAGTC CTGCAGAGCG CACTCACAAG 
GTGATTGCAC TGGTCAACAA GATGGTGAGC ATGATGGAGG GGGTGATTCA GACACAGAAG 
AATATCGCAG GGGCGCTTGC CTTCTGGATG GCCAATGCGT CTGAGCTCCT CAACTTTGTC 
AAACAGGACC GAGATCTTAG TCGGATCACA CTGGATGCCC AGGACGTTTT AGCACACTTG 
GTTCAGATGG CATTTAAATA TCTGGTTCAT TGTCTTCAAT CAGAACTGAA TAATTACATG 
CCAGCCTTTC TGGATGACCC TGAGGAGAAC AGCCTACAAA GACCAAAAAT AGATGATGTT 
CTGCACACGC TCACGGGAGC CATGTCTTTG CTGCGGCGCT GCAGAGTCAA TGCCGCCCTG 
ACCATCCAGC TCTTCTCCCA GCTCTTCCAC TTCGTCAACA TGTGGCTCTT CAACAGGCTG 
GTGACCGACC CCGAGTCAGG ACTGTGCTCT CACTACTGGG GGGCAATCAT CCGCCAGCAG 
CTGGGACACG TGGAAGCCTG GGCAGAGAAG CAGGGCTTGG AGCTGGCCGC CGACTGTCAC 
CTCAGCCGGA TAGTGCAGGC AACCACTTTG CTTACAATGG ATAAATACGC ACCTGATGAC 
ATTCCAAATA TAAACAGTAC CTGCTTTAAG TTAAACTCAC TGCAACTGCA AGCCTTGTTA 
CAGAATTACC ACTGTGCACC CGATGAGCCT TTTATCCCCA CGGACCTGAT AGAAAATGTG 
GTGGCTGTGG CTGAAAACAC GGCTGATGAG CTGGCGCGCA GTGACGGGAG GGAGGTGCAG 
CTCGAAGAGG ACCCCGATCT GCAGCTGCCT TTTCTTTTGC CAGAAGATGG CTATTCTTGT 
GATGTTGTCA GGAACATTCC AAATGGTTTA CAAGAATTTT TGGACCCTTT GTGCCAGAGA 
GGATTTTGCA GGCTCGTCCC TCACGCACGC TCACCAGGTA CTTGGACGAT ATATTTTGAA 
GGTGCAGATT ATGAGAGCCA CCTTCTGCGC GAGAGCACGG AGCTGGCTCA GCCTCTGAGG 
AAAGAACCTG AAATAATCAC TGTGACTCTT AAAAAGCAAA ATGGGATGGG CCTCAGCATT 
GTTGCAGCAA AGGGTGCTGG TCAAGATAAA CTCGGAATCT ATGTCAAGTC TGTTGTAAAA 
GGAGGTGCTG CGGATGTGGA TGGACGCCTG GCTGCAGGTG ACCAGCTCCT CAGTGTGGAC 
GGACGAAGTC TGGTAGGACT CTCTCAAGAA AGGGCGGCAG AACTCATGAC AAGAACAAGT 
TCTGTGGTAA CACTGGAAGT AGCAAAGCAG GGAGCAATTT ATCACGGCCT CGCCACCCTG 
CTCAATCAGC CGTCCCCGAT GATGCAAAGA ATTTCAGACC GTCGTGGCTC AGGTAAACCC 
CGACCAAAGA GTGAAGGTTT CGAGCTCTAT AATAATTCAG CTCCAAACGG GTCACCTGAG 
AGCCCTCAGC TGCCCTGGGC AGAATACAGT GAACCGAAGA AATTGCCAGG CGACGACAGG 
CTGATGAAGA ACCGGGCTGA TCACCGCTCT AGCCCCAACG TAGCAAATCA GCCTCCTAGC 
CCTGGAGGGA AGAGCACGTA CGCCCCCGGA ACAACAGCTA AGATAACGTC TGTCTCCACT 
GGGAACCTCT GCACTGAGGA ACAGACCCCT CCACCTAGAC CTGAGGCCTA CCCCATCCCC 
ACTCAGACGT ACACCAGAGA GTATTTCACC TTCCCAGCTT CCAAATCCCA GGACCGGATG 
GCTCCTCCTC AGAACCAGTG GCCAAATTAC GAGGAGAAGC CACACATGCA CCCAGACAGT 
AATCATTCCA GTATTGCAAT TCAGCGTGTT ACCCGTTCCC AAGAGGAACT TCGGGAGGAT 
AAAGCATACC AGCTAGAGAG GCATCAAATC GAGGCAGTCT TGGATCGCAA GTCTGATAGT 
GATATGTGGA TCCATCAGAG CTCCTCAGTG GGCTCCAGCA CCTCCAGCCA AGAGCACCTG 
GACCACTCCT CCAAGTCAGG CACCCCCGCG TCCACTCTGA CCAAGAGCGG CCCTGGGCGC 
TGGAAGACGC CAGCCGCTGT GCAGCCCATG CCCGCGGCCG TCTCCCAGCC TGTCCAAACA 
GATCCGCCCC CGCCACCGCC ACCGCCTCCC ACACACTATG CCGGAGACTT TGACGGAATC 
CCCGTGGACT TGCCTCTCCC TCCGCCTCCT GCCGGCCAGA CAGGACCCGC GTCTGCTCAG 
GCGGCGGCCG CAGAGCGGCG GAAGCGGGAG GAGCACCAGC GGTGGTATGA GAAGGAGAAG 
GCCCGTCTAG AAGAGGAGCG GGACAGGAAG CGCAGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGTCCCTGAA CCCGGCCCCG TTCTCTCCTG TGACCGTCCC ACACGTGAAG 
CCAGAAAAAC CGTCCACACT GCAGAGGCCC CAGGAGACGG TCATTCGGGA GCTGCAGCCC 
CAGCAGCAGC CCCGCACGAT TGAGCGCAGA GACTTACAGT ACATTACGGT CAGCAAAGAG 
GAGCTGTCGT CGGGAGACAG TCTGTCCCCA GACCCGTGGA AGCGGGATGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAGAT GCACATCGTG GATATGCTGA GCAGGGAGAT CCACGAGCTC 
CAGAGCAAGC CCGAGCGCAG TGCGGAGGAG AACGACCGCC TGCGGAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT CCAGGAGTCC AAACAGAAGG ACGAGGATGA CGAGGAAGAG 
GAGGACGATG ACGTGGATAC CATGCTGATC ATGCAGCGCC TTGAGGCTGA GCGGAGAGCA 
AGGGATGAGG AGCGGAGGCG CCAGCAGCAG CTGGAAGAGA TGCGCAAGCG GGAAGCCGAA 
GATCGCGCCA GGCAGGAGGA GGAGCGGCGC CGCCAGGAGG AGGAGAGAAC AAAGCGAGAT 
GCTGAAGAAA AGGTTATGGT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019799127.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019654686.1 Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OAc92449
Clone ID Related Accession (Same CDS sequence)	XM_019799127.1
Accession Version	XM_019799127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Ailuropoda melanoleuca(giant panda)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217569.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAGA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC CCTCTCAAAG 
AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TAAGGCAGGC GTCTGATAAG 
GATGATAGGC CTTTCCAAGG GGATGACGTT GAGAATTCCC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CTGAAACTAG CTTTACCCGA ACAATTTCGA ATCCGGAGGT GGTCATGAAG 
CGTCGCCGGC AGCAGAAACT AGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCTGACTCAG GTGGAACATT GAGAATTTAT GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCTAC CACAGACCCT GCAGAGTTTG CTGTGGCTGA AGCTTTAGAG 
AAATACGGCC TGGAGAAGGA GAATCCCAAG GAGTACTGCA TTGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCTGA CGAAAAAGGT GCTAAAGAAA TTATCCTTGA TGATGATGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCA AGTGACAAAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGAC CACCAGACTA TATCCCAAAG AAAACTAAGA AACACATCGA TGGGAAGCTG 
GTGAAGGGCA AGGAGAGAGC AGAGGGGTCG GGCTATGGCT CCGCACTTCC GCCCGAAAAG 
CTGCCCTATC TAGTAGAGCT GAGCCCAGAC GGCTCTGACT CCAGAGATAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT CACTGAGGTT GGGACAGAAA AGTTCGATGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGGGTT 
GTCACTGTCA CGCCAAGAAG CATGGATGCT GAGACTTATG TGGAAGGACA GCGCATCTCA 
GAGACAACCA TGCTGCAAAG TGGAATGAAA GTGCAGTTCG GGGCATCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCTCTG GCAAAGAGAT CTGTGGATGG AGGCCTGACA 
GCCAAGGGTC CCAGACATAA GCCTGGAATT GTTCAAGAGA CAACTTTTGA TTTAGGAGGA 
GATGTTCATA GTGGAACAGC ACTGCCAACA AGCAAGAGCA CCACGAGGCT GGACAGCGAC 
AGGGTGTTGT CTGCCTCCAG CACGGCGGAG CGCGGGATGG TGAAGCCAAT GATGAGAGTA 
GAACAGCAGC AGGATTACCG CAGGCAAGAA AACAGAACTC AGGACGCCCC TGGGCCTGAA 
CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACCGTC CATTTTAAGT TGTCACCTAC ATATGTATTG 
TATCTGGCAT GTCGGTATGT ATTGTCCAGC CAGTCCAGAG CTGACGTCAG TCCTGCAGAG 
CGCACTCACA AGGTGATTGC ACTGGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGACACAGA AGAATATCGC AGGGGCGCTT GCCTTCTGGA TGGCCAATGC GTCTGAGCTC 
CTCAACTTTG TCAAACAGGA CCGAGATCTT AGTCGGATCA CACTGGATGC CCAGGACGTT 
TTAGCACACT TGGTTCAGAT GGCATTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG 
AATAATTACA TGCCAGCCTT TCTGGATGAC CCTGAGGAGA ACAGCCTACA AAGACCAAAA 
ATAGATGATG TTCTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC 
AATGCCGCCC TGACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTCAACAGGC TGGTGACCGA CCCCGAGTCA GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGACA CGTGGAAGCC TGGGCAGAGA AGCAGGGCTT GGAGCTGGCC 
GCCGACTGTC ACCTCAGCCG GATAGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC 
GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAACTC ACTGCAACTG 
CAAGCCTTGT TACAGAATTA CCACTGTGCA CCCGATGAGC CTTTTATCCC CACGGACCTG 
ATAGAAAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGATG AGCTGGCGCG CAGTGACGGG 
AGGGAGGTGC AGCTCGAAGA GGACCCCGAT CTGCAGCTGC CTTTTCTTTT GCCAGAAGAT 
GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTGGACCCT 
TTGTGCCAGA GAGGATTTTG CAGGCTCGTC CCTCACGCAC GCTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CACCTTCTGC GCGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAAAAGCA AAATGGGATG 
GGCCTCAGCA TTGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTCGGAAT CTATGTCAAG 
TCTGTTGTAA AAGGAGGTGC TGCGGATGTG GATGGACGCC TGGCTGCAGG TGACCAGCTC 
CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAACTCATG 
ACAAGAACAA GTTCTGTGGT AACACTGGAA GTAGCAAAGC AGGGAGCAAT TTATCACGGC 
CTCGCCACCC TGCTCAATCA GCCGTCCCCG ATGATGCAAA GAATTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCCAAAC 
GGGTCACCTG AGAGCCCTCA GCTGCCCTGG GCAGAATACA GTGAACCGAA GAAATTGCCA 
GGCGACGACA GGCTGATGAA GAACCGGGCT GATCACCGCT CTAGCCCCAA CGTAGCAAAT 
CAGCCTCCTA GCCCTGGAGG GAAGAGCACG TACGCCCCCG GAACAACAGC TAAGATAACG 
TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCC 
TACCCCATCC CCACTCAGAC GTACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC 
CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG 
CACCCAGACA GTAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAAGAGGAA 
CTTCGGGAGG ATAAAGCATA CCAGCTAGAG AGGCATCAAA TCGAGGCAGT CTTGGATCGC 
AAGTCTGATA GTGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCCAG CACCTCCAGC 
CAAGAGCACC TGGACCACTC CTCCAAGTCA GGCACCCCCG CGTCCACTCT GACCAAGAGC 
GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCA TGCCCGCGGC CGTCTCCCAG 
CCTGTCCAAA CAGATCCGCC CCCGCCACCG CCACCGCCTC CCACACACTA TGCCGGAGAC 
TTTGACGGAA TCCCCGTGGA CTTGCCTCTC CCTCCGCCTC CTGCCGGCCA GACAGGACCC 
GCGTCTGCTC AGGCGGCGGC CGCAGAGCGG CGGAAGCGGG AGGAGCACCA GCGGTGGTAT 
GAGAAGGAGA AGGCCCGTCT AGAAGAGGAG CGGGACAGGA AGCGCAGGGA GCAGGAGCGG 
AAGCTGGGCC AGATGCGCTC CCAGTCCCTG AACCCGGCCC CGTTCTCTCC TGTGACCGTC 
CCACACGTGA AGCCAGAAAA ACCGTCCACA CTGCAGAGGC CCCAGGAGAC GGTCATTCGG 
GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACG 
GTCAGCAAAG AGGAGCTGTC GTCGGGAGAC AGTCTGTCCC CAGACCCGTG GAAGCGGGAT 
GCCAGGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG 
ATCCACGAGC TCCAGAGCAA GCCCGAGCGC AGTGCGGAGG AGAACGACCG CCTGCGGAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CCAAACAGAA GGACGAGGAT 
GACGAGGAAG AGGAGGACGA TGACGTGGAT ACCATGCTGA TCATGCAGCG CCTTGAGGCT 
GAGCGGAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG 
CGCAAGCGGG AAGCCGAAGA TCGCGCCAGG CAGGAGGAGG AGCGGCGCCG CCAGGAGGAG 
GAGAGAACAA AGCGAGATGC TGAAGAAAAG AGGCGACAAG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG 
GAGCCGGGTC TGTGCAGACC TCCGCTTCCG CGGGGCTACG AGCTCCCGTC CCTGTCCCCT 
CCGCCTGCCG CTCCTCCTCC CCCGCCTCAG CGGACCGCCT CCTCCCTCCA GGCGCAGGCC 
CTCTCCCCAG ACTCGGTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGCGGCGGAG 
GAGCACTGCG GCCTGGCAGG TCAGGATAAG CACTCTTGCA CGAGGAAGTC CCTCGGGCAC 
CTTCTTCTTC CCGCCGCCCT TAAGCCGCAG CAGCCCCCCT GCCCGCCGCG CCCGGCCTCC 
GATGGCATCT TTCTCTCCAA CTCATTCCAG CCACCCTCGG CCCGTGCCGA CAGTACTGCT 
CACAAAGCAG GCCAGCCCCC GCCCCCTCCA AAAAAACCAG GCTTCTGCCA GCCCAGCCAC 
TCGAAAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019654686.1
CDS	18..5543
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASDKDDRPFQGDDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPAEFAVAEALEKYGLEKENPKEYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGVLVFQLKRRPPDYIPKKTKKHIDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFDENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLTAKGPRHKPGIVQETTFDLGGDVHSGTALPTSKSTTRLDSDRVLSASSTAERGMVKPMMRVEQQQDYRRQENRTQDAPGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYLACRYVLSSQSRADVSPAERTHKVIALVNKMVSMMEGVIQTQKNIAGALAFWMANASELLNFVKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFVNMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLVPHARSPGTWTIYFEGADYESHLLRESTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSAPNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSTYAPGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHPDSNHSSIAIQRVTRSQEELREDKAYQLERHQIEAVLDRKSDSDMWIHQSSSVGSSTSSQEHLDHSSKSGTPASTLTKSGPGRWKTPAAVQPMPAAVSQPVQTDPPPPPPPPPTHYAGDFDGIPVDLPLPPPPAGQTGPASAQAAAAERRKREEHQRWYEKEKARLEEERDRKRREQERKLGQMRSQSLNPAPFSPVTVPHVKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIHELQSKPERSAEENDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLCRPPLPRGYELPSLSPPPAAPPPPPQRTASSLQAQALSPDSVYTARLVAYHEEAAEEHCGLAGQDKHSCTRKSLGHLLLPAALKPQQPPCPPRPASDGIFLSNSFQPPSARADSTAHKAGQPPPPPKKPGFCQPSHSKGPNSYMGSAGTAVGAHDAYRDPRERLSKSHDADLPGSPGAPENLTFRERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019799127.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019799127.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAATTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTGATC GAGACGCTGG CGGAGAAATT CCGGCCTGAC 
ATGCGGATGC TGTCATCCCC CAAGTACTCC CTCTATGAAG TGCATGTCAG TGGAGAAAGA 
AGATTGGATA TAGATGAGAA GCCCCTAGTT GTTCAGCTGA ATTGGAATAA AGATGATCGA 
GAGGGCAGAT TTGTTCTTAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAGA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC CCTCTCAAAG 
AAAGAAAAGA AGGAGAAAAA GAAGAGAGAA AAAGAGGCAG TAAGGCAGGC GTCTGATAAG 
GATGATAGGC CTTTCCAAGG GGATGACGTT GAGAATTCCC GACTGGCTGC CGAGGTTTAC 
AAAGACATGC CTGAAACTAG CTTTACCCGA ACAATTTCGA ATCCGGAGGT GGTCATGAAG 
CGTCGCCGGC AGCAGAAACT AGAGAGGAGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCTGACTCAG GTGGAACATT GAGAATTTAT GCAGATAGTT TAAAACCAAA TATTCCCTAC 
AAGACAATCC TGCTGTCTAC CACAGACCCT GCAGAGTTTG CTGTGGCTGA AGCTTTAGAG 
AAATACGGCC TGGAGAAGGA GAATCCCAAG GAGTACTGCA TTGCCCGGGT TATGCTTCCT 
CCTGGAGCCC AGCATTCTGA CGAAAAAGGT GCTAAAGAAA TTATCCTTGA TGATGATGAG 
TGTCCTTTAC AGATCTTCAG GGAATGGCCA AGTGACAAAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGAC CACCAGACTA TATCCCAAAG AAAACTAAGA AACACATCGA TGGGAAGCTG 
GTGAAGGGCA AGGAGAGAGC AGAGGGGTCG GGCTATGGCT CCGCACTTCC GCCCGAAAAG 
CTGCCCTATC TAGTAGAGCT GAGCCCAGAC GGCTCTGACT CCAGAGATAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT CACTGAGGTT GGGACAGAAA AGTTCGATGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGGGTT 
GTCACTGTCA CGCCAAGAAG CATGGATGCT GAGACTTATG TGGAAGGACA GCGCATCTCA 
GAGACAACCA TGCTGCAAAG TGGAATGAAA GTGCAGTTCG GGGCATCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCTCTG GCAAAGAGAT CTGTGGATGG AGGCCTGACA 
GCCAAGGGTC CCAGACATAA GCCTGGAATT GTTCAAGAGA CAACTTTTGA TTTAGGAGGA 
GATGTTCATA GTGGAACAGC ACTGCCAACA AGCAAGAGCA CCACGAGGCT GGACAGCGAC 
AGGGTGTTGT CTGCCTCCAG CACGGCGGAG CGCGGGATGG TGAAGCCAAT GATGAGAGTA 
GAACAGCAGC AGGATTACCG CAGGCAAGAA AACAGAACTC AGGACGCCCC TGGGCCTGAA 
CTGATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACCGTC CATTTTAAGT TGTCACCTAC ATATGTATTG 
TATCTGGCAT GTCGGTATGT ATTGTCCAGC CAGTCCAGAG CTGACGTCAG TCCTGCAGAG 
CGCACTCACA AGGTGATTGC ACTGGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGACACAGA AGAATATCGC AGGGGCGCTT GCCTTCTGGA TGGCCAATGC GTCTGAGCTC 
CTCAACTTTG TCAAACAGGA CCGAGATCTT AGTCGGATCA CACTGGATGC CCAGGACGTT 
TTAGCACACT TGGTTCAGAT GGCATTTAAA TATCTGGTTC ATTGTCTTCA ATCAGAACTG 
AATAATTACA TGCCAGCCTT TCTGGATGAC CCTGAGGAGA ACAGCCTACA AAGACCAAAA 
ATAGATGATG TTCTGCACAC GCTCACGGGA GCCATGTCTT TGCTGCGGCG CTGCAGAGTC 
AATGCCGCCC TGACCATCCA GCTCTTCTCC CAGCTCTTCC ACTTCGTCAA CATGTGGCTC 
TTCAACAGGC TGGTGACCGA CCCCGAGTCA GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGACA CGTGGAAGCC TGGGCAGAGA AGCAGGGCTT GGAGCTGGCC 
GCCGACTGTC ACCTCAGCCG GATAGTGCAG GCAACCACTT TGCTTACAAT GGATAAATAC 
GCACCTGATG ACATTCCAAA TATAAACAGT ACCTGCTTTA AGTTAAACTC ACTGCAACTG 
CAAGCCTTGT TACAGAATTA CCACTGTGCA CCCGATGAGC CTTTTATCCC CACGGACCTG 
ATAGAAAATG TGGTGGCTGT GGCTGAAAAC ACGGCTGATG AGCTGGCGCG CAGTGACGGG 
AGGGAGGTGC AGCTCGAAGA GGACCCCGAT CTGCAGCTGC CTTTTCTTTT GCCAGAAGAT 
GGCTATTCTT GTGATGTTGT CAGGAACATT CCAAATGGTT TACAAGAATT TTTGGACCCT 
TTGTGCCAGA GAGGATTTTG CAGGCTCGTC CCTCACGCAC GCTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CACCTTCTGC GCGAGAGCAC GGAGCTGGCT 
CAGCCTCTGA GGAAAGAACC TGAAATAATC ACTGTGACTC TTAAAAAGCA AAATGGGATG 
GGCCTCAGCA TTGTTGCAGC AAAGGGTGCT GGTCAAGATA AACTCGGAAT CTATGTCAAG 
TCTGTTGTAA AAGGAGGTGC TGCGGATGTG GATGGACGCC TGGCTGCAGG TGACCAGCTC 
CTCAGTGTGG ACGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCGGC AGAACTCATG 
ACAAGAACAA GTTCTGTGGT AACACTGGAA GTAGCAAAGC AGGGAGCAAT TTATCACGGC 
CTCGCCACCC TGCTCAATCA GCCGTCCCCG ATGATGCAAA GAATTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGTGAAGGT TTCGAGCTCT ATAATAATTC AGCTCCAAAC 
GGGTCACCTG AGAGCCCTCA GCTGCCCTGG GCAGAATACA GTGAACCGAA GAAATTGCCA 
GGCGACGACA GGCTGATGAA GAACCGGGCT GATCACCGCT CTAGCCCCAA CGTAGCAAAT 
CAGCCTCCTA GCCCTGGAGG GAAGAGCACG TACGCCCCCG GAACAACAGC TAAGATAACG 
TCTGTCTCCA CTGGGAACCT CTGCACTGAG GAACAGACCC CTCCACCTAG ACCTGAGGCC 
TACCCCATCC CCACTCAGAC GTACACCAGA GAGTATTTCA CCTTCCCAGC TTCCAAATCC 
CAGGACCGGA TGGCTCCTCC TCAGAACCAG TGGCCAAATT ACGAGGAGAA GCCACACATG 
CACCCAGACA GTAATCATTC CAGTATTGCA ATTCAGCGTG TTACCCGTTC CCAAGAGGAA 
CTTCGGGAGG ATAAAGCATA CCAGCTAGAG AGGCATCAAA TCGAGGCAGT CTTGGATCGC 
AAGTCTGATA GTGATATGTG GATCCATCAG AGCTCCTCAG TGGGCTCCAG CACCTCCAGC 
CAAGAGCACC TGGACCACTC CTCCAAGTCA GGCACCCCCG CGTCCACTCT GACCAAGAGC 
GGCCCTGGGC GCTGGAAGAC GCCAGCCGCT GTGCAGCCCA TGCCCGCGGC CGTCTCCCAG 
CCTGTCCAAA CAGATCCGCC CCCGCCACCG CCACCGCCTC CCACACACTA TGCCGGAGAC 
TTTGACGGAA TCCCCGTGGA CTTGCCTCTC CCTCCGCCTC CTGCCGGCCA GACAGGACCC 
GCGTCTGCTC AGGCGGCGGC CGCAGAGCGG CGGAAGCGGG AGGAGCACCA GCGGTGGTAT 
GAGAAGGAGA AGGCCCGTCT AGAAGAGGAG CGGGACAGGA AGCGCAGGGA GCAGGAGCGG 
AAGCTGGGCC AGATGCGCTC CCAGTCCCTG AACCCGGCCC CGTTCTCTCC TGTGACCGTC 
CCACACGTGA AGCCAGAAAA ACCGTCCACA CTGCAGAGGC CCCAGGAGAC GGTCATTCGG 
GAGCTGCAGC CCCAGCAGCA GCCCCGCACG ATTGAGCGCA GAGACTTACA GTACATTACG 
GTCAGCAAAG AGGAGCTGTC GTCGGGAGAC AGTCTGTCCC CAGACCCGTG GAAGCGGGAT 
GCCAGGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGATATGCT GAGCAGGGAG 
ATCCACGAGC TCCAGAGCAA GCCCGAGCGC AGTGCGGAGG AGAACGACCG CCTGCGGAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CCAAACAGAA GGACGAGGAT 
GACGAGGAAG AGGAGGACGA TGACGTGGAT ACCATGCTGA TCATGCAGCG CCTTGAGGCT 
GAGCGGAGAG CAAGGTTACA GGATGAGGAG CGGAGGCGCC AGCAGCAGCT GGAAGAGATG 
CGCAAGCGGG AAGCCGAAGA TCGCGCCAGG CAGGAGGAGG AGCGGCGCCG CCAGGAGGAG 
GAGAGAACAA AGCGAGATGC TGAAGAAAAG AGGCGACAAG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGTTGCT GGAGCCGGAG 
GAGCCGGGTC TGTGCAGACC TCCGCTTCCG CGGGGCTACG AGCTCCCGTC CCTGTCCCCT 
CCGCCTGCCG CTCCTCCTCC CCCGCCTCAG CGGACCGCCT CCTCCCTCCA GGCGCAGGCC 
CTCTCCCCAG ACTCGGTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGCGGCGGAG 
GAGCACTGCG GCCTGGCAGG TCAGGATAAG CACTCTTGCA CGAGGAAGTC CCTCGGGCAC 
CTTCTTCTTC CCGCCGCCCT TAAGCCGCAG CAGCCCCCCT GCCCGCCGCG CCCGGCCTCC 
GATGGCATCT TTCTCTCCAA CTCATTCCAG CCACCCTCGG CCCGTGCCGA CAGTACTGCT 
CACAAAGCAG GCCAGCCCCC GCCCCCTCCA AAAAAACCAG GCTTCTGCCA GCCCAGCCAC 
TCGAAAGGAC CAAACTCTTA CATGGGATCG GCTGGGACAG CTGTTGGTGC CCATGATGCT 
TATCGGGATC CGAGAGAGAG ACTGTCTAAA AGCCACGATG CAGATTTACC TGGGAGTCCG 
GGAGCCCCTG AGAACTTGAC GTTCAGAGAG CGCCAGCGCC TGTTTTCACA AGGTCAAGAT 
GTGTCCAACA AAGTAAAAGC CTCTCGTAAA TTAACAGAAC TGGAAAACGA ACTGAACACA 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFDN
Entrez Gene ID	100483095
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFDN cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

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