PDHB cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDHB
Entrez Gene ID	408654
Full Name	pyruvate dehydrogenase (lipoamide) beta
Synonyms	GB17238
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001242513.1	NP_001229442.1	pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00236	NM_001242513.1 Latest version!	Apis mellifera pyruvate dehydrogenase (lipoamide) beta (PDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDHB ( NM_001242513.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001229442.1 Apis mellifera pyruvate dehydrogenase (lipoamide) beta (PDHB), mRNA.

Price & Availability↑

CloneID	OHd00236
Clone ID Related Accession (Same CDS sequence)	NM_001242513.1
Accession Version	NM_001242513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Apis mellifera(honey bee)
Product	pyruvate dehydrogenase E1 component subunit beta, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from HP549405.1. On Jun 14, 2011 this sequence version replaced XR_015064.2. ##Evidence-Data-START## Transcript exon combination :: HP549405.1, HP537189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00113341 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTTTCGT CGATTGTAAC GAGAGTTATC CGACGTTCGT TTTCAACGTC AAAATGGAAT 
GCCGTGCAGC AGATGACGGT ACGAGATGCT TTAAATTCAG CTTTGGATGA AGAAATGGAA 
AGAGACGAAA GAGTATTTTT ACTTGGAGAA GAAGTAGCGT TGTATGATGG AGCCTATAAA 
GTTTCGAGGG GCCTCTGGAA AAAATATGGA GATAAACGTG TTATTGATAC TCCTATCACG 
GAAGCAGGAT TTGCTGGTAT AGCTGTTGGA GCAGCTATGG CTGGTTTACG TCCTGTATGT 
GAATTTATGA CATTCAATTT TTCTATGCAA GCAATCGATC AAATTATTAA TTCTGCCGCA 
AAGACATTTT ATATGTCTGC TGGACGAGTA AATGTACCAG TTGTTTTTCG TGGACCTAAT 
GGGGCAGCAG CCGGAGTGGC AGCACAACAT TCGCAATGTT TTGGTGCTTG GTATAGTCAT 
TGTCCTGGTT TAAAAGTAGT ATCACCCTAT ACTAGCGAAG ATGCAAAAGG ATTGTTAAAA 
GCTGCCATTC GAGATCCTGA TCCAGTTGTT GTATTAGAAA ATGAAATATT ATATGGTGTT 
CAATACCCTA TGTCTGACGA AGCTTTATCA AAAGATTTTA TTTTGCCAAT CGGTAAAGCG 
AAGATTGAAC GTGCTGGCAA ACACGTTACA TTAGTGGCAC ATTCCAAAGC AGTTGAACAA 
GCTCTTCAAG CAGCGAATGA ACTTGCAGGA AAAGGAATAG AGGCAGAAGT AATAAACCTT 
AGATCATTAA GACCTTTAGA TATAGATACC ATTGTACAAT CAGTAGTGAA AACGAATTAC 
GTTGTAACTG TGGAACAAGG TTGGCCGCAA TGTGGTATCG GTGCGGAGAT TAGTGCTAGG 
ATTTCGGAAA GTGAAGCTTT CTATCATCTT GATGCACCAG TAATGCGCGT TACAGGTGTT 
GATTCACCTA TGCCTTATGC TAGAACTTTA GAAAATGCAT CTCTGCCACA AGCGAATGAT 
ATTGTGTATA CAGTAAATAA AATACTAGGA TTGGTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229442.1
CDS	144..1205
Translation	MFSSIVTRVIRRSFSTSKWNAVQQMTVRDALNSALDEEMERDERVFLLGEEVALYDGAYKVSRGLWKKYGDKRVIDTPITEAGFAGIAVGAAMAGLRPVCEFMTFNFSMQAIDQIINSAAKTFYMSAGRVNVPVVFRGPNGAAAGVAAQHSQCFGAWYSHCPGLKVVSPYTSEDAKGLLKAAIRDPDPVVVLENEILYGVQYPMSDEALSKDFILPIGKAKIERAGKHVTLVAHSKAVEQALQAANELAGKGIEAEVINLRSLRPLDIDTIVQSVVKTNYVVTVEQGWPQCGIGAEISARISESEAFYHLDAPVMRVTGVDSPMPYARTLENASLPQANDIVYTVNKILGLVQ

Target ORF information:

RefSeq Version	NM_001242513.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera pyruvate dehydrogenase (lipoamide) beta (PDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242513.1

ORF Insert Sequence:

ATGTTTTCGT CGATTGTAAC GAGAGTTATC CGACGTTCGT TTTCAACGTC AAAATGGAAT 
GCCGTGCAGC AGATGACGGT ACGAGATGCT TTAAATTCAG CTTTGGATGA AGAAATGGAA 
AGAGACGAAA GAGTATTTTT ACTTGGAGAA GAAGTAGCGT TGTATGATGG AGCCTATAAA 
GTTTCGAGGG GCCTCTGGAA AAAATATGGA GATAAACGTG TTATTGATAC TCCTATCACG 
GAAGCAGGAT TTGCTGGTAT AGCTGTTGGA GCAGCTATGG CTGGTTTACG TCCTGTATGT 
GAATTTATGA CATTCAATTT TTCTATGCAA GCAATCGATC AAATTATTAA TTCTGCCGCA 
AAGACATTTT ATATGTCTGC TGGACGAGTA AATGTACCAG TTGTTTTTCG TGGACCTAAT 
GGGGCAGCAG CCGGAGTGGC AGCACAACAT TCGCAATGTT TTGGTGCTTG GTATAGTCAT 
TGTCCTGGTT TAAAAGTAGT ATCACCCTAT ACTAGCGAAG ATGCAAAAGG ATTGTTAAAA 
GCTGCCATTC GAGATCCTGA TCCAGTTGTT GTATTAGAAA ATGAAATATT ATATGGTGTT 
CAATACCCTA TGTCTGACGA AGCTTTATCA AAAGATTTTA TTTTGCCAAT CGGTAAAGCG 
AAGATTGAAC GTGCTGGCAA ACACGTTACA TTAGTGGCAC ATTCCAAAGC AGTTGAACAA 
GCTCTTCAAG CAGCGAATGA ACTTGCAGGA AAAGGAATAG AGGCAGAAGT AATAAACCTT 
AGATCATTAA GACCTTTAGA TATAGATACC ATTGTACAAT CAGTAGTGAA AACGAATTAC 
GTTGTAACTG TGGAACAAGG TTGGCCGCAA TGTGGTATCG GTGCGGAGAT TAGTGCTAGG 
ATTTCGGAAA GTGAAGCTTT CTATCATCTT GATGCACCAG TAATGCGCGT TACAGGTGTT 
GATTCACCTA TGCCTTATGC TAGAACTTTA GAAAATGCAT CTCTGCCACA AGCGAATGAT 
ATTGTGTATA CAGTAAATAA AATACTAGGA TTGGTACAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)