RASA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	RASA1
Entrez Gene ID	282032
Full Name	RAS p21 protein activator 1
General protein information	Preferred Names RAS p21 protein activator 1 Names ras GTPase-activating protein 1 GAP GTPase-activating protein RAS p21 protein activator (GTPase activating protein RAS p21) p120GAP rasGAP
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 7 Map Location: 7q24-qter

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807597.2	XP_010805899.1	ras GTPase-activating protein 1 isoform X2
XM_005209729.3	XP_005209786.1	ras GTPase-activating protein 1 isoform X1
XM_005209729.4	XP_005209786.2	ras GTPase-activating protein 1 isoform X1
XM_010807597.1	XP_010805899.1	ras GTPase-activating protein 1 isoform X2
NM_174449.2	NP_776874.1	ras GTPase-activating protein 1

Pathways from BioSystems

Homologies

	RASA1	XP_001084074.2
Xenopus tropicalis (tropical clawed frog)	LOC100135092	NP_001107303.1
Pan troglodytes (chimpanzee)	RASA1	XP_517663.3
Canis lupus familiaris (dog)	RASA1	XP_536302.3
Bos taurus (cattle)	RASA1	NP_776874.1
Mus musculus (house mouse)	Rasa1	NP_663427.2
Rattus norvegicus (Norway rat)	Rasa1	NP_037267.1
Gallus gallus (chicken)	RASA1	XP_424907.4
Danio rerio (zebrafish)	rasa1a	XP_001342009.2
Drosophila melanogaster (fruit fly)	vap	NP_727926.2
Homo sapiens (human)	RASA1	NP_002881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002729	XP_312197.3

Gene Ontology
Bibliography

Related articles in PubMed

S-nitrosylation decreases the adsorption of H-Ras in lipid bilayer and changes intrinsic catalytic activity.
Shanshiashvili L, Narmania N, Barbakadze T, Zhuravliova E, Natsvlishvili N, Ramsden J, Mikeladze DG
Cell biochemistry and biophysics59(3)191-9(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning of bovine GAP and its interaction with oncogenic ras p21.
Vogel US, Dixon RA, Schaber MD, Diehl RE, Marshall MS, Scolnick EM, Sigal IS, Gibbs JB
Nature335(6185)90-3(1988 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The data suggest that nitrosylation of H-Ras rearranges the adsorptive potential and intrinsic GTPase activity of H-Ras through modification of C-terminal cysteines of molecule.
Title: S-nitrosylation decreases the adsorption of H-Ras in lipid bilayer and changes intrinsic catalytic activity.

The following RASA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa45316	XM_010807597.2 Latest version!	Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa235768	XM_005209729.4 Latest version!	Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa41590	NM_174449.2 Latest version!	Bos taurus RAS p21 protein activator 1 (RASA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa21753	XM_005209729.3 Latest version!	Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBa45316	XM_010807597.1	Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASA1 ( XM_010807597.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805899.1 Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45316
Clone ID Related Accession (Same CDS sequence)	XM_010807597.2 , XM_010807597.1
Accession Version	XM_010807597.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ras GTPase-activating protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG ATTCTTTCTT GCATCTGGTA GCATTTACCT TCAGAAGAAG ACTGATTTTC 
ACTTTCAGTG TGAAAAGCCC TGCTTGTACC CTGTACCATG GCATGACCAG TATTGACTGG 
GCTACATTCT TCAAAGAAAA CTCCTCAGGA GCTGTGTTGT TGTGGTATCA TGGAAAACTT 
GATAGAACTA TAGCAGAAGA ACGCCTCAGG CAAGCAGGGA AGTCCGGCAG TTACCTTATA 
AGAGAGAGTG ATCGGAGGCC AGGGTCCTTT GTACTTTCCT TTCTTAGCCA GACGAACGTT 
GTCAACCATT TTAGGATTAT TGCTATGTGT GGAGATTACT ACATTGGTGG AAGACGTTTT 
TCTTCTCTGT CAGACCTAAT AGGTTATTAC AGTCATGTTT CCTGTTTGCT TAAAGGAGAA 
AAACTGCTTT ATCCAGTTGC ACCGCCAGAG CCAGTAGAAG ATAGAAGGCG TGTCCGAGCC 
ATTCTCCCTT ACACAAAAGT ACCAGACACT GATGAAATAA GTTTCTTAAA AGGAGATATG 
TTCATTGTTC ATAACGAATT AGAAGATGGA TGGATGTGGG TTACAAATCT AAGAACAGAT 
GAACAAGGTC TTATTGTTGA AGACTTAGTA GAAGAGGTGG GCCGGGAAGA AGATCCACAT 
GAAGGCAAAA TATGGTTCCA TGGGAAAATT TCCAAACAAG AAGCTTATAA TTTACTAATG 
ACAGTTGGTC AAGTCTGCAG TTTTCTTGTG AGGCCGTCAG ATAATACTCC TGGGGACTAT 
TCACTTTATT TTCGGACCAG TGAAAATATT CAGCGGTTTA AAATATGTCC AACACCAAAC 
AATCAGTTTA TGATGGGAGG CCGATATTAT AACAGCATTG GGGACATCAT AGATCACTAT 
CGAAAAGAAC AAATTGTTGA AGGGTATTAT CTTAAGGAAC CTGTACCCAT GCAGGATCAA 
GAACAAGTAC TGAATGATGC AGTGGATGGC AAGGAAATTT ATAATACAAT TCGTCGTAAA 
ACAAAGGATG CCTTTTATAA AAATATTGTT AAAAAAGGTT ATCTTCTAAA AAAAGGAAAG 
GGAAAAAGGT GGAAAAATTT ATATTTTATC TTAGAGGGCA GTGATGCCCA ACTTATTTAT 
TTTGAAAGTG AAAAACGAGC TACAAAACCA AAAGGATTAA TAGATCTCAG CGTGTGTTCT 
GTCTATGTTG TTCATGATAG TCTCTTTGGC AGGCCAAACT GTTTCCAGAT AGTGGTTCAG 
CACTTTAGTG AAGAACATTA CATCTTCTAC TTTGCAGGAG AAACTCCAGA ACAGGCAGAG 
GATTGGATGA AAGGTCTGCA GGCATTTTGC AACTTAAGGA AAAGTAGTCC AGGGACATCT 
AATAAACGCC TGCGTCAGGT CAGCAGTCTT ATTTTACATA TTGAAGAAGC CCATAAACTC 
CCAGTAAAGC ATTTTACTAA TCCATATTGT AACATCTACC TGAATAGCGT CCAGGTAGCA 
AAAACTCATG CAAGGGAAGG GCAAAACCCT GTATGGTCAG AAGAGTTTGT CTTTGATGAT 
CTTCCTCCTG ACATCAACAG ATTTGAAATA ACTCTTAGTA ATAAAACGAA GAAAAGCAAA 
GATCCTGATA TCTTATTTAT GCGCTGCCAG TTGAGCCGGT TACAGAAGGG GCATGCCACA 
GACGAATGGT TTTTGCTCAG CTCCCATATA CCATTGAAAG GTATTGAACC AGGATCCCTG 
CGTGTCCGAG CACGATATTC TATGGAAAAG ATCATGCCAG AAGAAGAGTA CAGCGAATTT 
AAGGAGCTTA TACTGCAAAA GGAACTTCAT GTAGTCTATG CTTTATCACA TGTATGTGGA 
CAGGACCGAA CACTACTCGC TAGCATTCTA CTGAAGATTT TTCTTCATGA AAAGCTTGAA 
TCATTGTTGT TATGTACACT AAATGACAGA GAAATAAGCA TGGAAGATGA AGCCACTACC 
CTCTTTCGAG CCACAACACT TGCGAGCACC TTGATGGAGC AGTATATGAA AGCCACTGCT 
ACACAATTTG TTCATCACGC TTTAAAAGAC TCTATCTTAA GGATAATGGA AAGCAAGCAG 
TCTTGTGAGT TAAGTCCATC AAAGTTAGAA AAAAATGAAG ATGTGAACAC TAATTTAGCA 
CACCTATTGA ACATACTTTC AGAGCTTGTG GAGAAAATAT TCATGGCTTC AGAAATACTT 
CCACCGACAC TGAGGTATAT TTATGGGTGT TTACAGAAAT CTGTTCAGCA CAAGTGGCCT 
ACAAATACCA CCATGAGAAC AAGAGTTGTT AGTGGTTTTG TTTTTCTTCG ACTTATCTGT 
CCTGCCATCC TGAATCCACG GATGTTTAAT ATCATCTCAG ATTCCCCATC TCCTATTGCT 
GCAAGAACAC TGACATTAGT GGCTAAGTCT GTGCAGAACT TAGCAAATCT TGTGGAATTT 
GGAGCTAAGG AGCCTTACAT GGAAGGTGTC AATCCATTCA TCAAAAGTAA TAAACATCGC 
ATGATCATGT TTTTAGATGA ACTAGGGAAT GTACCTGAAC TTCCGGACAC TACAGAGCAT 
TCTAGAACTG ACCTTTGCCG TGATTTAGCA GCATTGCATG AGATTTGTGT GGCCCATTCA 
GATGAACTGC GGACGCTCAG TAATGAGCGT GGTGCACAAC AGCATGTACT GAAAAAGCTT 
CTGGCTATAA CAGAACTGCT TCAGCAAAAA CAAAACCAGT ATACAAAAAC TAACGATGTC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805899.1
CDS	16..2781
Translation	MNKDSFLHLVAFTFRRRLIFTFSVKSPACTLYHGMTSIDWATFFKENSSGAVLLWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTSENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDAVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLILHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILRIMESKQSCELSPSKLEKNEDVNTNLAHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLCRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	XM_010807597.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807597.2

ORF Insert Sequence:

ATGAACAAAG ATTCTTTCTT GCATCTGGTA GCATTTACCT TCAGAAGAAG ACTGATTTTC 
ACTTTCAGTG TGAAAAGCCC TGCTTGTACC CTGTACCATG GCATGACCAG TATTGACTGG 
GCTACATTCT TCAAAGAAAA CTCCTCAGGA GCTGTGTTGT TGTGGTATCA TGGAAAACTT 
GATAGAACTA TAGCAGAAGA ACGCCTCAGG CAAGCAGGGA AGTCCGGCAG TTACCTTATA 
AGAGAGAGTG ATCGGAGGCC AGGGTCCTTT GTACTTTCCT TTCTTAGCCA GACGAACGTT 
GTCAACCATT TTAGGATTAT TGCTATGTGT GGAGATTACT ACATTGGTGG AAGACGTTTT 
TCTTCTCTGT CAGACCTAAT AGGTTATTAC AGTCATGTTT CCTGTTTGCT TAAAGGAGAA 
AAACTGCTTT ATCCAGTTGC ACCGCCAGAG CCAGTAGAAG ATAGAAGGCG TGTCCGAGCC 
ATTCTCCCTT ACACAAAAGT ACCAGACACT GATGAAATAA GTTTCTTAAA AGGAGATATG 
TTCATTGTTC ATAACGAATT AGAAGATGGA TGGATGTGGG TTACAAATCT AAGAACAGAT 
GAACAAGGTC TTATTGTTGA AGACTTAGTA GAAGAGGTGG GCCGGGAAGA AGATCCACAT 
GAAGGCAAAA TATGGTTCCA TGGGAAAATT TCCAAACAAG AAGCTTATAA TTTACTAATG 
ACAGTTGGTC AAGTCTGCAG TTTTCTTGTG AGGCCGTCAG ATAATACTCC TGGGGACTAT 
TCACTTTATT TTCGGACCAG TGAAAATATT CAGCGGTTTA AAATATGTCC AACACCAAAC 
AATCAGTTTA TGATGGGAGG CCGATATTAT AACAGCATTG GGGACATCAT AGATCACTAT 
CGAAAAGAAC AAATTGTTGA AGGGTATTAT CTTAAGGAAC CTGTACCCAT GCAGGATCAA 
GAACAAGTAC TGAATGATGC AGTGGATGGC AAGGAAATTT ATAATACAAT TCGTCGTAAA 
ACAAAGGATG CCTTTTATAA AAATATTGTT AAAAAAGGTT ATCTTCTAAA AAAAGGAAAG 
GGAAAAAGGT GGAAAAATTT ATATTTTATC TTAGAGGGCA GTGATGCCCA ACTTATTTAT 
TTTGAAAGTG AAAAACGAGC TACAAAACCA AAAGGATTAA TAGATCTCAG CGTGTGTTCT 
GTCTATGTTG TTCATGATAG TCTCTTTGGC AGGCCAAACT GTTTCCAGAT AGTGGTTCAG 
CACTTTAGTG AAGAACATTA CATCTTCTAC TTTGCAGGAG AAACTCCAGA ACAGGCAGAG 
GATTGGATGA AAGGTCTGCA GGCATTTTGC AACTTAAGGA AAAGTAGTCC AGGGACATCT 
AATAAACGCC TGCGTCAGGT CAGCAGTCTT ATTTTACATA TTGAAGAAGC CCATAAACTC 
CCAGTAAAGC ATTTTACTAA TCCATATTGT AACATCTACC TGAATAGCGT CCAGGTAGCA 
AAAACTCATG CAAGGGAAGG GCAAAACCCT GTATGGTCAG AAGAGTTTGT CTTTGATGAT 
CTTCCTCCTG ACATCAACAG ATTTGAAATA ACTCTTAGTA ATAAAACGAA GAAAAGCAAA 
GATCCTGATA TCTTATTTAT GCGCTGCCAG TTGAGCCGGT TACAGAAGGG GCATGCCACA 
GACGAATGGT TTTTGCTCAG CTCCCATATA CCATTGAAAG GTATTGAACC AGGATCCCTG 
CGTGTCCGAG CACGATATTC TATGGAAAAG ATCATGCCAG AAGAAGAGTA CAGCGAATTT 
AAGGAGCTTA TACTGCAAAA GGAACTTCAT GTAGTCTATG CTTTATCACA TGTATGTGGA 
CAGGACCGAA CACTACTCGC TAGCATTCTA CTGAAGATTT TTCTTCATGA AAAGCTTGAA 
TCATTGTTGT TATGTACACT AAATGACAGA GAAATAAGCA TGGAAGATGA AGCCACTACC 
CTCTTTCGAG CCACAACACT TGCGAGCACC TTGATGGAGC AGTATATGAA AGCCACTGCT 
ACACAATTTG TTCATCACGC TTTAAAAGAC TCTATCTTAA GGATAATGGA AAGCAAGCAG 
TCTTGTGAGT TAAGTCCATC AAAGTTAGAA AAAAATGAAG ATGTGAACAC TAATTTAGCA 
CACCTATTGA ACATACTTTC AGAGCTTGTG GAGAAAATAT TCATGGCTTC AGAAATACTT 
CCACCGACAC TGAGGTATAT TTATGGGTGT TTACAGAAAT CTGTTCAGCA CAAGTGGCCT 
ACAAATACCA CCATGAGAAC AAGAGTTGTT AGTGGTTTTG TTTTTCTTCG ACTTATCTGT 
CCTGCCATCC TGAATCCACG GATGTTTAAT ATCATCTCAG ATTCCCCATC TCCTATTGCT 
GCAAGAACAC TGACATTAGT GGCTAAGTCT GTGCAGAACT TAGCAAATCT TGTGGAATTT 
GGAGCTAAGG AGCCTTACAT GGAAGGTGTC AATCCATTCA TCAAAAGTAA TAAACATCGC 
ATGATCATGT TTTTAGATGA ACTAGGGAAT GTACCTGAAC TTCCGGACAC TACAGAGCAT 
TCTAGAACTG ACCTTTGCCG TGATTTAGCA GCATTGCATG AGATTTGTGT GGCCCATTCA 
GATGAACTGC GGACGCTCAG TAATGAGCGT GGTGCACAAC AGCATGTACT GAAAAAGCTT 
CTGGCTATAA CAGAACTGCT TCAGCAAAAA CAAAACCAGT ATACAAAAAC TAACGATGTC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA1 ( XM_010807597.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805899.1 Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45316
Clone ID Related Accession (Same CDS sequence)	XM_010807597.2 , XM_010807597.1
Accession Version	XM_010807597.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ras GTPase-activating protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAAG ATTCTTTCTT GCATCTGGTA GCATTTACCT TCAGAAGAAG ACTGATTTTC 
ACTTTCAGTG TGAAAAGCCC TGCTTGTACC CTGTACCATG GCATGACCAG TATTGACTGG 
GCTACATTCT TCAAAGAAAA CTCCTCAGGA GCTGTGTTGT TGTGGTATCA TGGAAAACTT 
GATAGAACTA TAGCAGAAGA ACGCCTCAGG CAAGCAGGGA AGTCCGGCAG TTACCTTATA 
AGAGAGAGTG ATCGGAGGCC AGGGTCCTTT GTACTTTCCT TTCTTAGCCA GACGAACGTT 
GTCAACCATT TTAGGATTAT TGCTATGTGT GGAGATTACT ACATTGGTGG AAGACGTTTT 
TCTTCTCTGT CAGACCTAAT AGGTTATTAC AGTCATGTTT CCTGTTTGCT TAAAGGAGAA 
AAACTGCTTT ATCCAGTTGC ACCGCCAGAG CCAGTAGAAG ATAGAAGGCG TGTCCGAGCC 
ATTCTCCCTT ACACAAAAGT ACCAGACACT GATGAAATAA GTTTCTTAAA AGGAGATATG 
TTCATTGTTC ATAACGAATT AGAAGATGGA TGGATGTGGG TTACAAATCT AAGAACAGAT 
GAACAAGGTC TTATTGTTGA AGACTTAGTA GAAGAGGTGG GCCGGGAAGA AGATCCACAT 
GAAGGCAAAA TATGGTTCCA TGGGAAAATT TCCAAACAAG AAGCTTATAA TTTACTAATG 
ACAGTTGGTC AAGTCTGCAG TTTTCTTGTG AGGCCGTCAG ATAATACTCC TGGGGACTAT 
TCACTTTATT TTCGGACCAG TGAAAATATT CAGCGGTTTA AAATATGTCC AACACCAAAC 
AATCAGTTTA TGATGGGAGG CCGATATTAT AACAGCATTG GGGACATCAT AGATCACTAT 
CGAAAAGAAC AAATTGTTGA AGGGTATTAT CTTAAGGAAC CTGTACCCAT GCAGGATCAA 
GAACAAGTAC TGAATGATGC AGTGGATGGC AAGGAAATTT ATAATACAAT TCGTCGTAAA 
ACAAAGGATG CCTTTTATAA AAATATTGTT AAAAAAGGTT ATCTTCTAAA AAAAGGAAAG 
GGAAAAAGGT GGAAAAATTT ATATTTTATC TTAGAGGGCA GTGATGCCCA ACTTATTTAT 
TTTGAAAGTG AAAAACGAGC TACAAAACCA AAAGGATTAA TAGATCTCAG CGTGTGTTCT 
GTCTATGTTG TTCATGATAG TCTCTTTGGC AGGCCAAACT GTTTCCAGAT AGTGGTTCAG 
CACTTTAGTG AAGAACATTA CATCTTCTAC TTTGCAGGAG AAACTCCAGA ACAGGCAGAG 
GATTGGATGA AAGGTCTGCA GGCATTTTGC AACTTAAGGA AAAGTAGTCC AGGGACATCT 
AATAAACGCC TGCGTCAGGT CAGCAGTCTT ATTTTACATA TTGAAGAAGC CCATAAACTC 
CCAGTAAAGC ATTTTACTAA TCCATATTGT AACATCTACC TGAATAGCGT CCAGGTAGCA 
AAAACTCATG CAAGGGAAGG GCAAAACCCT GTATGGTCAG AAGAGTTTGT CTTTGATGAT 
CTTCCTCCTG ACATCAACAG ATTTGAAATA ACTCTTAGTA ATAAAACGAA GAAAAGCAAA 
GATCCTGATA TCTTATTTAT GCGCTGCCAG TTGAGCCGGT TACAGAAGGG GCATGCCACA 
GACGAATGGT TTTTGCTCAG CTCCCATATA CCATTGAAAG GTATTGAACC AGGATCCCTG 
CGTGTCCGAG CACGATATTC TATGGAAAAG ATCATGCCAG AAGAAGAGTA CAGCGAATTT 
AAGGAGCTTA TACTGCAAAA GGAACTTCAT GTAGTCTATG CTTTATCACA TGTATGTGGA 
CAGGACCGAA CACTACTCGC TAGCATTCTA CTGAAGATTT TTCTTCATGA AAAGCTTGAA 
TCATTGTTGT TATGTACACT AAATGACAGA GAAATAAGCA TGGAAGATGA AGCCACTACC 
CTCTTTCGAG CCACAACACT TGCGAGCACC TTGATGGAGC AGTATATGAA AGCCACTGCT 
ACACAATTTG TTCATCACGC TTTAAAAGAC TCTATCTTAA GGATAATGGA AAGCAAGCAG 
TCTTGTGAGT TAAGTCCATC AAAGTTAGAA AAAAATGAAG ATGTGAACAC TAATTTAGCA 
CACCTATTGA ACATACTTTC AGAGCTTGTG GAGAAAATAT TCATGGCTTC AGAAATACTT 
CCACCGACAC TGAGGTATAT TTATGGGTGT TTACAGAAAT CTGTTCAGCA CAAGTGGCCT 
ACAAATACCA CCATGAGAAC AAGAGTTGTT AGTGGTTTTG TTTTTCTTCG ACTTATCTGT 
CCTGCCATCC TGAATCCACG GATGTTTAAT ATCATCTCAG ATTCCCCATC TCCTATTGCT 
GCAAGAACAC TGACATTAGT GGCTAAGTCT GTGCAGAACT TAGCAAATCT TGTGGAATTT 
GGAGCTAAGG AGCCTTACAT GGAAGGTGTC AATCCATTCA TCAAAAGTAA TAAACATCGC 
ATGATCATGT TTTTAGATGA ACTAGGGAAT GTACCTGAAC TTCCGGACAC TACAGAGCAT 
TCTAGAACTG ACCTTTGCCG TGATTTAGCA GCATTGCATG AGATTTGTGT GGCCCATTCA 
GATGAACTGC GGACGCTCAG TAATGAGCGT GGTGCACAAC AGCATGTACT GAAAAAGCTT 
CTGGCTATAA CAGAACTGCT TCAGCAAAAA CAAAACCAGT ATACAAAAAC TAACGATGTC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805899.1
CDS	16..2781
Translation	MNKDSFLHLVAFTFRRRLIFTFSVKSPACTLYHGMTSIDWATFFKENSSGAVLLWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTSENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDAVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLILHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILRIMESKQSCELSPSKLEKNEDVNTNLAHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLCRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	XM_010807597.1
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807597.1

ORF Insert Sequence:

ATGAACAAAG ATTCTTTCTT GCATCTGGTA GCATTTACCT TCAGAAGAAG ACTGATTTTC 
ACTTTCAGTG TGAAAAGCCC TGCTTGTACC CTGTACCATG GCATGACCAG TATTGACTGG 
GCTACATTCT TCAAAGAAAA CTCCTCAGGA GCTGTGTTGT TGTGGTATCA TGGAAAACTT 
GATAGAACTA TAGCAGAAGA ACGCCTCAGG CAAGCAGGGA AGTCCGGCAG TTACCTTATA 
AGAGAGAGTG ATCGGAGGCC AGGGTCCTTT GTACTTTCCT TTCTTAGCCA GACGAACGTT 
GTCAACCATT TTAGGATTAT TGCTATGTGT GGAGATTACT ACATTGGTGG AAGACGTTTT 
TCTTCTCTGT CAGACCTAAT AGGTTATTAC AGTCATGTTT CCTGTTTGCT TAAAGGAGAA 
AAACTGCTTT ATCCAGTTGC ACCGCCAGAG CCAGTAGAAG ATAGAAGGCG TGTCCGAGCC 
ATTCTCCCTT ACACAAAAGT ACCAGACACT GATGAAATAA GTTTCTTAAA AGGAGATATG 
TTCATTGTTC ATAACGAATT AGAAGATGGA TGGATGTGGG TTACAAATCT AAGAACAGAT 
GAACAAGGTC TTATTGTTGA AGACTTAGTA GAAGAGGTGG GCCGGGAAGA AGATCCACAT 
GAAGGCAAAA TATGGTTCCA TGGGAAAATT TCCAAACAAG AAGCTTATAA TTTACTAATG 
ACAGTTGGTC AAGTCTGCAG TTTTCTTGTG AGGCCGTCAG ATAATACTCC TGGGGACTAT 
TCACTTTATT TTCGGACCAG TGAAAATATT CAGCGGTTTA AAATATGTCC AACACCAAAC 
AATCAGTTTA TGATGGGAGG CCGATATTAT AACAGCATTG GGGACATCAT AGATCACTAT 
CGAAAAGAAC AAATTGTTGA AGGGTATTAT CTTAAGGAAC CTGTACCCAT GCAGGATCAA 
GAACAAGTAC TGAATGATGC AGTGGATGGC AAGGAAATTT ATAATACAAT TCGTCGTAAA 
ACAAAGGATG CCTTTTATAA AAATATTGTT AAAAAAGGTT ATCTTCTAAA AAAAGGAAAG 
GGAAAAAGGT GGAAAAATTT ATATTTTATC TTAGAGGGCA GTGATGCCCA ACTTATTTAT 
TTTGAAAGTG AAAAACGAGC TACAAAACCA AAAGGATTAA TAGATCTCAG CGTGTGTTCT 
GTCTATGTTG TTCATGATAG TCTCTTTGGC AGGCCAAACT GTTTCCAGAT AGTGGTTCAG 
CACTTTAGTG AAGAACATTA CATCTTCTAC TTTGCAGGAG AAACTCCAGA ACAGGCAGAG 
GATTGGATGA AAGGTCTGCA GGCATTTTGC AACTTAAGGA AAAGTAGTCC AGGGACATCT 
AATAAACGCC TGCGTCAGGT CAGCAGTCTT ATTTTACATA TTGAAGAAGC CCATAAACTC 
CCAGTAAAGC ATTTTACTAA TCCATATTGT AACATCTACC TGAATAGCGT CCAGGTAGCA 
AAAACTCATG CAAGGGAAGG GCAAAACCCT GTATGGTCAG AAGAGTTTGT CTTTGATGAT 
CTTCCTCCTG ACATCAACAG ATTTGAAATA ACTCTTAGTA ATAAAACGAA GAAAAGCAAA 
GATCCTGATA TCTTATTTAT GCGCTGCCAG TTGAGCCGGT TACAGAAGGG GCATGCCACA 
GACGAATGGT TTTTGCTCAG CTCCCATATA CCATTGAAAG GTATTGAACC AGGATCCCTG 
CGTGTCCGAG CACGATATTC TATGGAAAAG ATCATGCCAG AAGAAGAGTA CAGCGAATTT 
AAGGAGCTTA TACTGCAAAA GGAACTTCAT GTAGTCTATG CTTTATCACA TGTATGTGGA 
CAGGACCGAA CACTACTCGC TAGCATTCTA CTGAAGATTT TTCTTCATGA AAAGCTTGAA 
TCATTGTTGT TATGTACACT AAATGACAGA GAAATAAGCA TGGAAGATGA AGCCACTACC 
CTCTTTCGAG CCACAACACT TGCGAGCACC TTGATGGAGC AGTATATGAA AGCCACTGCT 
ACACAATTTG TTCATCACGC TTTAAAAGAC TCTATCTTAA GGATAATGGA AAGCAAGCAG 
TCTTGTGAGT TAAGTCCATC AAAGTTAGAA AAAAATGAAG ATGTGAACAC TAATTTAGCA 
CACCTATTGA ACATACTTTC AGAGCTTGTG GAGAAAATAT TCATGGCTTC AGAAATACTT 
CCACCGACAC TGAGGTATAT TTATGGGTGT TTACAGAAAT CTGTTCAGCA CAAGTGGCCT 
ACAAATACCA CCATGAGAAC AAGAGTTGTT AGTGGTTTTG TTTTTCTTCG ACTTATCTGT 
CCTGCCATCC TGAATCCACG GATGTTTAAT ATCATCTCAG ATTCCCCATC TCCTATTGCT 
GCAAGAACAC TGACATTAGT GGCTAAGTCT GTGCAGAACT TAGCAAATCT TGTGGAATTT 
GGAGCTAAGG AGCCTTACAT GGAAGGTGTC AATCCATTCA TCAAAAGTAA TAAACATCGC 
ATGATCATGT TTTTAGATGA ACTAGGGAAT GTACCTGAAC TTCCGGACAC TACAGAGCAT 
TCTAGAACTG ACCTTTGCCG TGATTTAGCA GCATTGCATG AGATTTGTGT GGCCCATTCA 
GATGAACTGC GGACGCTCAG TAATGAGCGT GGTGCACAAC AGCATGTACT GAAAAAGCTT 
CTGGCTATAA CAGAACTGCT TCAGCAAAAA CAAAACCAGT ATACAAAAAC TAACGATGTC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA1 ( XM_005209729.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209786.2 Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235768
Clone ID Related Accession (Same CDS sequence)	XM_005209729.4
Accession Version	XM_005209729.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ras GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209729.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GACCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGTCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTATATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209786.2
CDS	1..3135
Translation	MMAAEAGGEEGGPVTAGAAGGGAAAASGAYPAVCRVKIPAALPVAAAAPFPGLAEAGVAATLGGGAALGSGFLGPGSVAGTPGGVGLSAGGAAAGVAGVAAAAAGAGGEMAFAKGTTSLPTETFGAGGGFPPLPPPPPQLPTLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTSENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDAVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLILHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILRIMESKQSCELSPSKLEKNEDVNTNLAHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLCRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	XM_005209729.4
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS p21 protein activator 1 (RASA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209729.4

ORF Insert Sequence:

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GACCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGTCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTATATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA1 ( NM_174449.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776874.1 Bos taurus RAS p21 protein activator 1 (RASA1), mRNA.

Price & Availability↑

CloneID	OBa41590
Clone ID Related Accession (Same CDS sequence)	NM_174449.2
Accession Version	NM_174449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	ras GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X12602.1. On or before Mar 29, 2005 this sequence version replaced XM_598642.1, NM_174449.1. ##Evidence-Data-START## Transcript exon combination :: X12602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GAGCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGCCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTCTATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776874.1
CDS	598..3732
Translation	MMAAEAGGEEGGPVTAGAAGGGAAAASGAYPAVCRVKIPAALPVAAAAPFPGLAEAGVAATLGGGAALGSGFLGAGSVAGTPGGVGLSAGGAAAGVAGVAAAAAGAGGEMAFAKGTTSLPTETFGAGGGFPPLPPPPPQLPTLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQACSFLVRPSDNTPGDYSLYFRTSENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDAVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLILHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQSMKATATQFVHHALKDSILRIMESKQSCELSPSKLEKNEDVNTNLAHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLCRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	NM_174449.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS p21 protein activator 1 (RASA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174449.2

ORF Insert Sequence:

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GAGCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGCCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTCTATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASA1 ( XM_005209729.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209786.1 Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X1, mRNA.

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CloneID	OBa21753
Clone ID Related Accession (Same CDS sequence)	XM_005209729.3
Accession Version	XM_005209729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ras GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GAGCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGTCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTATATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209786.1
CDS	541..3675
Translation	MMAAEAGGEEGGPVTAGAAGGGAAAASGAYPAVCRVKIPAALPVAAAAPFPGLAEAGVAATLGGGAALGSGFLGAGSVAGTPGGVGLSAGGAAAGVAGVAAAAAGAGGEMAFAKGTTSLPTETFGAGGGFPPLPPPPPQLPTLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTSENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDAVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLILHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILRIMESKQSCELSPSKLEKNEDVNTNLAHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLCRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQKQNQYTKTNDVR

Target ORF information:

RefSeq Version	XM_005209729.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209729.3

ORF Insert Sequence:

ATGATGGCGG CCGAGGCCGG CGGTGAGGAG GGCGGCCCGG TCACCGCCGG GGCAGCGGGA 
GGCGGCGCGG CCGCGGCCTC GGGTGCCTAC CCGGCGGTGT GTCGGGTGAA GATCCCCGCG 
GCCCTGCCCG TGGCCGCCGC CGCCCCCTTT CCTGGGCTGG CGGAGGCCGG CGTGGCCGCG 
ACTCTAGGTG GCGGAGCCGC TCTGGGGTCA GGCTTCCTGG GAGCTGGGTC TGTGGCGGGG 
ACCCCGGGGG GAGTCGGACT GTCAGCCGGA GGCGCTGCCG CCGGCGTGGC TGGTGTCGCC 
GCCGCCGCCG CCGGAGCCGG CGGGGAGATG GCTTTCGCCA AGGGGACCAC TTCGTTGCCT 
ACTGAGACCT TCGGGGCCGG CGGCGGATTC CCTCCTCTGC CGCCGCCTCC TCCTCAGTTG 
CCCACTTTGG GCGCTGGCCT GGGAACAGTG GACGAAGGTG ACTCTCTGGA TGGACCAGAA 
TACGAGGAGG AAGAGGTGGC CATCCCGCTG ACCGCTCCTC CGACTAACCA GTGGTATCAT 
GGAAAACTTG ATAGAACTAT AGCAGAAGAA CGCCTCAGGC AAGCAGGGAA GTCCGGCAGT 
TACCTTATAA GAGAGAGTGA TCGGAGGCCA GGGTCCTTTG TACTTTCCTT TCTTAGCCAG 
ACGAACGTTG TCAACCATTT TAGGATTATT GCTATGTGTG GAGATTACTA CATTGGTGGA 
AGACGTTTTT CTTCTCTGTC AGACCTAATA GGTTATTACA GTCATGTTTC CTGTTTGCTT 
AAAGGAGAAA AACTGCTTTA TCCAGTTGCA CCGCCAGAGC CAGTAGAAGA TAGAAGGCGT 
GTCCGAGCCA TTCTCCCTTA CACAAAAGTA CCAGACACTG ATGAAATAAG TTTCTTAAAA 
GGAGATATGT TCATTGTTCA TAACGAATTA GAAGATGGAT GGATGTGGGT TACAAATCTA 
AGAACAGATG AACAAGGTCT TATTGTTGAA GACTTAGTAG AAGAGGTGGG CCGGGAAGAA 
GATCCACATG AAGGCAAAAT ATGGTTCCAT GGGAAAATTT CCAAACAAGA AGCTTATAAT 
TTACTAATGA CAGTTGGTCA AGTCTGCAGT TTTCTTGTGA GGCCGTCAGA TAATACTCCT 
GGGGACTATT CACTTTATTT TCGGACCAGT GAAAATATTC AGCGGTTTAA AATATGTCCA 
ACACCAAACA ATCAGTTTAT GATGGGAGGC CGATATTATA ACAGCATTGG GGACATCATA 
GATCACTATC GAAAAGAACA AATTGTTGAA GGGTATTATC TTAAGGAACC TGTACCCATG 
CAGGATCAAG AACAAGTACT GAATGATGCA GTGGATGGCA AGGAAATTTA TAATACAATT 
CGTCGTAAAA CAAAGGATGC CTTTTATAAA AATATTGTTA AAAAAGGTTA TCTTCTAAAA 
AAAGGAAAGG GAAAAAGGTG GAAAAATTTA TATTTTATCT TAGAGGGCAG TGATGCCCAA 
CTTATTTATT TTGAAAGTGA AAAACGAGCT ACAAAACCAA AAGGATTAAT AGATCTCAGC 
GTGTGTTCTG TCTATGTTGT TCATGATAGT CTCTTTGGCA GGCCAAACTG TTTCCAGATA 
GTGGTTCAGC ACTTTAGTGA AGAACATTAC ATCTTCTACT TTGCAGGAGA AACTCCAGAA 
CAGGCAGAGG ATTGGATGAA AGGTCTGCAG GCATTTTGCA ACTTAAGGAA AAGTAGTCCA 
GGGACATCTA ATAAACGCCT GCGTCAGGTC AGCAGTCTTA TTTTACATAT TGAAGAAGCC 
CATAAACTCC CAGTAAAGCA TTTTACTAAT CCATATTGTA ACATCTACCT GAATAGCGTC 
CAGGTAGCAA AAACTCATGC AAGGGAAGGG CAAAACCCTG TATGGTCAGA AGAGTTTGTC 
TTTGATGATC TTCCTCCTGA CATCAACAGA TTTGAAATAA CTCTTAGTAA TAAAACGAAG 
AAAAGCAAAG ATCCTGATAT CTTATTTATG CGCTGCCAGT TGAGCCGGTT ACAGAAGGGG 
CATGCCACAG ACGAATGGTT TTTGCTCAGC TCCCATATAC CATTGAAAGG TATTGAACCA 
GGATCCCTGC GTGTCCGAGC ACGATATTCT ATGGAAAAGA TCATGCCAGA AGAAGAGTAC 
AGCGAATTTA AGGAGCTTAT ACTGCAAAAG GAACTTCATG TAGTCTATGC TTTATCACAT 
GTATGTGGAC AGGACCGAAC ACTACTCGCT AGCATTCTAC TGAAGATTTT TCTTCATGAA 
AAGCTTGAAT CATTGTTGTT ATGTACACTA AATGACAGAG AAATAAGCAT GGAAGATGAA 
GCCACTACCC TCTTTCGAGC CACAACACTT GCGAGCACCT TGATGGAGCA GTATATGAAA 
GCCACTGCTA CACAATTTGT TCATCACGCT TTAAAAGACT CTATCTTAAG GATAATGGAA 
AGCAAGCAGT CTTGTGAGTT AAGTCCATCA AAGTTAGAAA AAAATGAAGA TGTGAACACT 
AATTTAGCAC ACCTATTGAA CATACTTTCA GAGCTTGTGG AGAAAATATT CATGGCTTCA 
GAAATACTTC CACCGACACT GAGGTATATT TATGGGTGTT TACAGAAATC TGTTCAGCAC 
AAGTGGCCTA CAAATACCAC CATGAGAACA AGAGTTGTTA GTGGTTTTGT TTTTCTTCGA 
CTTATCTGTC CTGCCATCCT GAATCCACGG ATGTTTAATA TCATCTCAGA TTCCCCATCT 
CCTATTGCTG CAAGAACACT GACATTAGTG GCTAAGTCTG TGCAGAACTT AGCAAATCTT 
GTGGAATTTG GAGCTAAGGA GCCTTACATG GAAGGTGTCA ATCCATTCAT CAAAAGTAAT 
AAACATCGCA TGATCATGTT TTTAGATGAA CTAGGGAATG TACCTGAACT TCCGGACACT 
ACAGAGCATT CTAGAACTGA CCTTTGCCGT GATTTAGCAG CATTGCATGA GATTTGTGTG 
GCCCATTCAG ATGAACTGCG GACGCTCAGT AATGAGCGTG GTGCACAACA GCATGTACTG 
AAAAAGCTTC TGGCTATAAC AGAACTGCTT CAGCAAAAAC AAAACCAGTA TACAAAAACT 
AACGATGTCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

S-nitrosylation decreases the adsorption of H-Ras in lipid bilayer and changes intrinsic catalytic activity.
Cell biochemistry and biophysics59(3)191-9(2011 Apr)
Shanshiashvili L,Narmania N,Barbakadze T,Zhuravliova E,Natsvlishvili N,Ramsden J,Mikeladze DG

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning of bovine GAP and its interaction with oncogenic ras p21.
Nature335(6185)90-3(1988 Sep)
Vogel US,Dixon RA,Schaber MD,Diehl RE,Marshall MS,Scolnick EM,Sigal IS,Gibbs JB

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RASA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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