TIMP3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMP3
Entrez Gene ID	282094
Full Name	TIMP metallopeptidase inhibitor 3
Synonyms	TIMP-3
General protein information	Preferred Names TIMP metallopeptidase inhibitor 3 Names metalloproteinase inhibitor 3 TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory) tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory) tissue inhibitor of metalloproteinases 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174473.4	NP_776898.2	metalloproteinase inhibitor 3 precursor

Pathways from BioSystems

Homologies

	TIMP3	NP_001028128.1
Canis lupus familiaris (dog)	TIMP3	XP_538410.2
Rattus norvegicus (Norway rat)	Timp3	NP_037018.2
Gallus gallus (chicken)	TIMP3	NP_990818.1
Homo sapiens (human)	TIMP3	NP_000353.1
Pan troglodytes (chimpanzee)	TIMP3	XP_515097.2
Bos taurus (cattle)	TIMP3	NP_776898.2
Mus musculus (house mouse)	Timp3	NP_035725.1
Xenopus tropicalis (tropical clawed frog)	timp3	XP_002940366.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Effects of simple and complex motion patterns on gene expression of chondrocytes seeded in 3D scaffolds.
Grad S, Gogolewski S, Alini M, Wimmer MA
Tissue engineering12(11)3171-9(2006 Nov)

Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
The Journal of cell biology175(1)179-91(2006 Oct)

Transforming growth factor Beta1 induction of tissue inhibitor of metalloproteinases 3 in articular chondrocytes is mediated by reactive oxygen species.
Li WQ, Qureshi HY, Liacini A, Dehnade F, Zafarullah M
Free radical biology & medicine37(2)196-207(2004 Jul)

Coordinate expression of matrix-degrading proteinases and their activators and inhibitors in bovine skeletal muscle.
Balcerzak D, Querengesser L, Dixon WT, Baracos VE
Journal of animal science79(1)94-107(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TIMP3 mRNA expression level was upregulated by multidirectional articular motion.
Title: Effects of simple and complex motion patterns on gene expression of chondrocytes seeded in 3D scaffolds.

TIMP3 has a role in the pericyte-induced stabilization of newly formed vascular networks that are predisposed to undergo regression and reveal specific molecular targets of the inhibitors regulating these events.
Title: Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.

Reactive oxygen species mediate TGF-beta1-induced TIMP-3 gene expression
Title: Transforming growth factor Beta1 induction of tissue inhibitor of metalloproteinases 3 in articular chondrocytes is mediated by reactive oxygen species.

The following TIMP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01063	NM_174473.4 Latest version!	Bos taurus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMP3 ( NM_174473.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776898.2 Bos taurus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.

Price & Availability↑

CloneID	OBa01063
Clone ID Related Accession (Same CDS sequence)	NM_174473.4
Accession Version	NM_174473.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	metalloproteinase inhibitor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC112663.1. On Aug 31, 2012 this sequence version replaced NM_174473.3. ##Evidence-Data-START## Transcript exon combination :: BC112663.1, U77588.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGACTCCCT GGCTCGGGCT CGTCGTGCTC CTGGGCAGCT GGAGCCTGGG GGACTGGGGC 
GCTGAGGCGT GCACATGCTC GCCTAGCCAC CCCCAGGACG CGTTCTGCAA CTCAGACATC 
GTGATCCGAG CCAAGGTGGT AGGGAAGAAA CTGCTGAAGG AGGGGCCCTT TGGCACGATG 
GTCTACACCA TCAAGCAGAT GAAGATGTAC CGAGGATTCA CCAAGATGCC CCATGTGCAG 
TACATCCACA CAGAAGCTTC TGAAAGTCTC TGTGGCCTTA AGCTTGAGGT CAACAAGTAC 
CAGTACCTGC TGACAGGCCG AGTCTATGAT GGCAAGATGT ACACAGGACT GTGTAACTTT 
GTAGAGAGGT GGGACCAGCT CACCCTCTCC CAGCGCAAGG GGCTGAACTA TCGATATCAC 
CTGGGCTGTA ACTGCAAGAT CAAATCCTGC TACTACCTGC CTTGCTTTGT AACCACCAAG 
AACGAGTGTC TCTGGACCGA CATGCTCTCC AATTTCGGCT ACCCTGGCTA CCAGTCCAAA 
CACTACGCTT GCATCCGGCA GAAGGGTGGC TACTGTAGCT GGTACCGAGG ATGGGCACCC 
CCGGACAAAA GCATCATCAA TGCCACAGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776898.2
CDS	285..920
Translation	MTPWLGLVVLLGSWSLGDWGAEACTCSPSHPQDAFCNSDIVIRAKVVGKKLLKEGPFGTMVYTIKQMKMYRGFTKMPHVQYIHTEASESLCGLKLEVNKYQYLLTGRVYDGKMYTGLCNFVERWDQLTLSQRKGLNYRYHLGCNCKIKSCYYLPCFVTTKNECLWTDMLSNFGYPGYQSKHYACIRQKGGYCSWYRGWAPPDKSIINATDP

Target ORF information:

RefSeq Version	NM_174473.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TIMP metallopeptidase inhibitor 3 (TIMP3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174473.4

ORF Insert Sequence:

ATGACTCCCT GGCTCGGGCT CGTCGTGCTC CTGGGCAGCT GGAGCCTGGG GGACTGGGGC 
GCTGAGGCGT GCACATGCTC GCCTAGCCAC CCCCAGGACG CGTTCTGCAA CTCAGACATC 
GTGATCCGAG CCAAGGTGGT AGGGAAGAAA CTGCTGAAGG AGGGGCCCTT TGGCACGATG 
GTCTACACCA TCAAGCAGAT GAAGATGTAC CGAGGATTCA CCAAGATGCC CCATGTGCAG 
TACATCCACA CAGAAGCTTC TGAAAGTCTC TGTGGCCTTA AGCTTGAGGT CAACAAGTAC 
CAGTACCTGC TGACAGGCCG AGTCTATGAT GGCAAGATGT ACACAGGACT GTGTAACTTT 
GTAGAGAGGT GGGACCAGCT CACCCTCTCC CAGCGCAAGG GGCTGAACTA TCGATATCAC 
CTGGGCTGTA ACTGCAAGAT CAAATCCTGC TACTACCTGC CTTGCTTTGT AACCACCAAG 
AACGAGTGTC TCTGGACCGA CATGCTCTCC AATTTCGGCT ACCCTGGCTA CCAGTCCAAA 
CACTACGCTT GCATCCGGCA GAAGGGTGGC TACTGTAGCT GGTACCGAGG ATGGGCACCC 
CCGGACAAAA GCATCATCAA TGCCACAGAC CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Effects of simple and complex motion patterns on gene expression of chondrocytes seeded in 3D scaffolds.
Tissue engineering12(11)3171-9(2006 Nov)
Grad S,Gogolewski S,Alini M,Wimmer MA

Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
The Journal of cell biology175(1)179-91(2006 Oct)
Saunders WB,Bohnsack BL,Faske JB,Anthis NJ,Bayless KJ,Hirschi KK,Davis GE

Transforming growth factor Beta1 induction of tissue inhibitor of metalloproteinases 3 in articular chondrocytes is mediated by reactive oxygen species.
Free radical biology & medicine37(2)196-207(2004 Jul)
Li WQ,Qureshi HY,Liacini A,Dehnade F,Zafarullah M

Coordinate expression of matrix-degrading proteinases and their activators and inhibitors in bovine skeletal muscle.
Journal of animal science79(1)94-107(2001 Jan)
Balcerzak D,Querengesser L,Dixon WT,Baracos VE