ARF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	ARF1
Entrez Gene ID	338058
Full Name	ADP ribosylation factor 1
General protein information	Preferred Names ADP ribosylation factor 1 Names ADP-ribosylation factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005208399.4	XP_005208456.1	ADP-ribosylation factor 1 isoform X1
XM_005208399.3	XP_005208456.1	ADP-ribosylation factor 1 isoform X1
XM_005208400.3	XP_005208457.1	ADP-ribosylation factor 1 isoform X1
XM_005208400.4	XP_005208457.1	ADP-ribosylation factor 1 isoform X1
NM_176653.4	NP_788826.1	ADP-ribosylation factor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARF1	XP_001145129.1
Bos taurus (cattle)	ARF1	NP_788826.1
Gallus gallus (chicken)	ARF1	NP_001006352.1
Pan troglodytes (chimpanzee)	LOC457177	XP_513698.2
Canis lupus familiaris (dog)	LOC607365	NP_001238856.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012014	XP_320516.2
Homo sapiens (human)	ARF1	NP_001019399.1
Mus musculus (house mouse)	Arf1	NP_031502.1
Rattus norvegicus (Norway rat)	Arf1	NP_071963.1
Xenopus tropicalis (tropical clawed frog)	arf5	NP_989018.1
	ARF1	XP_002801945.1
Caenorhabditis elegans (roundworm)	arf-1.2	NP_498235.1
Danio rerio (zebrafish)	arf1	NP_958860.1
Drosophila melanogaster (fruit fly)	Arf79F	NP_730760.1

Gene Ontology
Bibliography

Related articles in PubMed

Vascular endothelial growth factor receptor-2 activates ADP-ribosylation factor 1 to promote endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells.
Daher Z, Boulay PL, Desjardins F, Gratton JP, Claing A
The Journal of biological chemistry285(32)24591-9(2010 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.
Lee I, Doray B, Govero J, Kornfeld S
The Journal of cell biology180(3)467-72(2008 Feb)

ARF1-mediated actin polymerization produces movement of artificial vesicles.
Heuvingh J, Franco M, Chavrier P, Sykes C
Proceedings of the National Academy of Sciences of the United States of America104(43)16928-33(2007 Oct)

Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
Haynes LP, Fitzgerald DJ, Wareing B, O'Callaghan DW, Morgan A, Burgoyne RD
Proteomics6(6)1822-32(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vascular endothelial growth factor receptor-2 activates ADP-ribosylation factor 1 to promote endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells
Title: Vascular endothelial growth factor receptor-2 activates ADP-ribosylation factor 1 to promote endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells.

Binding of cargo signal peptides to AP-1 induces a conformational change in its core domain that greatly enhances its interaction with Arf-1-GTP.
Title: Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.

The ARF1 machinery also might produce movement- and fission-promoting forces through actin polymerization.
Title: ARF1-mediated actin polymerization produces movement of artificial vesicles.

NCS-1-ARF1 interaction provides evidence for functional cross-talk between Ca(2+)-dependent and ARF-dependent pathways in TGN to plasma membrane traffic
Title: Interaction of neuronal calcium sensor-1 and ADP-ribosylation factor 1 allows bidirectional control of phosphatidylinositol 4-kinase beta and trans-Golgi network-plasma membrane traffic.

Data show that AP-1 recruitment to the cell membrane was found to be dependent on myristoylated ADP-ribosylation factor (ARF1), GTP or nonhydrolyzable GTP-analogs, tyrosine signals, and small amounts of phosphoinositides.
Title: ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes.

The following ARF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa20756	NM_176653.4 Latest version!	Bos taurus ADP ribosylation factor 1 (ARF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa20756	XM_005208400.4 Latest version!	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa20756	XM_005208399.4 Latest version!	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa20756	XM_005208399.3	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa20756	XM_005208400.3	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARF1 ( NM_176653.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_788826.1 Bos taurus ADP ribosylation factor 1 (ARF1), mRNA.

Price & Availability↑

CloneID	OBa20756
Clone ID Related Accession (Same CDS sequence)	XM_005208399.4 , XM_005208399.3 , XM_005208400.3 , XM_005208400.4 , NM_176653.4
Accession Version	NM_176653.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	ADP-ribosylation factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102874.1. On Aug 30, 2012 this sequence version replaced NM_176653.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC102874.1, J03275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788826.1
CDS	92..637
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	NM_176653.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor 1 (ARF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176653.4

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( XM_005208399.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208456.1 Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20756
Clone ID Related Accession (Same CDS sequence)	XM_005208399.4 , XM_005208399.3 , XM_005208400.3 , XM_005208400.4 , NM_176653.4
Accession Version	XM_005208399.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208399.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208456.1
CDS	125..670
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_005208399.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208399.4

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( XM_005208399.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208456.1 Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa20756
Clone ID Related Accession (Same CDS sequence)	XM_005208399.4 , XM_005208399.3 , XM_005208400.3 , XM_005208400.4 , NM_176653.4
Accession Version	XM_005208399.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104000.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208399.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208456.1
CDS	89..634
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_005208399.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208399.3

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( XM_005208400.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208457.1 Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20756
Clone ID Related Accession (Same CDS sequence)	XM_005208399.4 , XM_005208399.3 , XM_005208400.3 , XM_005208400.4 , NM_176653.4
Accession Version	XM_005208400.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104000.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208457.1
CDS	157..702
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_005208400.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208400.3

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF1 ( XM_005208400.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208457.1 Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20756
Clone ID Related Accession (Same CDS sequence)	XM_005208399.4 , XM_005208399.3 , XM_005208400.3 , XM_005208400.4 , NM_176653.4
Accession Version	XM_005208400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ADP-ribosylation factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208457.1
CDS	182..727
Translation	MGNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Target ORF information:

RefSeq Version	XM_005208400.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor 1 (ARF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208400.4

ORF Insert Sequence:

ATGGGGAATA TCTTTGCAAA CCTCTTCAAG GGCCTTTTTG GCAAAAAAGA AATGCGCATT 
CTCATGGTGG GCCTAGATGC TGCGGGAAAG ACCACCATCC TGTACAAACT GAAGCTGGGT 
GAAATTGTGA CCACCATTCC CACTATTGGC TTCAACGTGG AGACCGTGGA GTACAAGAAC 
ATCAGCTTCA CTGTGTGGGA CGTGGGTGGC CAGGACAAGA TCCGGCCTCT GTGGCGCCAC 
TACTTTCAAA ACACACAAGG TCTCATCTTT GTGGTTGACA GCAATGACAG AGAGCGTGTG 
AATGAGGCCC GTGAGGAGCT CATGAGGATG TTGGCAGAAG ACGAGCTCAG GGACGCCGTT 
CTGCTCGTGT TTGCTAACAA ACAGGACCTC CCTAATGCCA TGAACGCGGC CGAGATCACG 
GACAAGCTGG GCCTGCACTC TCTGCGCCAC AGGAACTGGT ACATTCAGGC CACCTGTGCC 
ACCAGTGGGG ACGGGCTTTA CGAGGGACTG GACTGGCTGT CCAATCAGCT CCGGAACCAG 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vascular endothelial growth factor receptor-2 activates ADP-ribosylation factor 1 to promote endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells.
The Journal of biological chemistry285(32)24591-9(2010 Aug)
Daher Z,Boulay PL,Desjardins F,Gratton JP,Claing A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.
The Journal of cell biology180(3)467-72(2008 Feb)
Lee I,Doray B,Govero J,Kornfeld S

ARF1-mediated actin polymerization produces movement of artificial vesicles.
Proceedings of the National Academy of Sciences of the United States of America104(43)16928-33(2007 Oct)
Heuvingh J,Franco M,Chavrier P,Sykes C

Analysis of the interacting partners of the neuronal calcium-binding proteins L-CaBP1, hippocalcin, NCS-1 and neurocalcin delta.
Proteomics6(6)1822-32(2006 Mar)
Haynes LP,Fitzgerald DJ,Wareing B,O'Callaghan DW,Morgan A,Burgoyne RD

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ARF1 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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