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LAMB3 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol LAMB3
Entrez Gene ID 529939
Full Name laminin subunit beta 3
General protein information
Preferred Names

laminin subunit beta 3

Names

laminin subunit beta-3
laminin, beta 3
Gene Type protein-coding
Organism Bos taurus(cattle)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_015475275.1 XP_015330761.1 laminin subunit beta-3 isoform X1
XM_015475276.1 XP_015330762.1 laminin subunit beta-3 isoform X1
XM_015475277.1 XP_015330763.1 laminin subunit beta-3 isoform X1
XM_015475277.2 XP_015330763.2 laminin subunit beta-3 isoform X1
XM_015475280.1 XP_015330766.1 laminin subunit beta-3 isoform X1
XM_015475281.1 XP_015330767.1 laminin subunit beta-3 isoform X2
NM_001081596.1 NP_001075065.1 laminin subunit beta-3 precursor
XM_010813565.2 XP_010811867.1 laminin subunit beta-3 isoform X1
XM_015475274.1 XP_015330760.1 laminin subunit beta-3 isoform X1
XM_005217367.3 XP_005217424.1 laminin subunit beta-3 isoform X1
XM_015475279.1 XP_015330765.1 laminin subunit beta-3 isoform X1
XM_010813564.3 XP_010811866.2 laminin subunit beta-3 isoform X1
XM_010813565.3 XP_010811867.2 laminin subunit beta-3 isoform X1
XM_015475274.2 XP_015330760.2 laminin subunit beta-3 isoform X1
XM_005217367.4 XP_005217424.2 laminin subunit beta-3 isoform X1
XM_010813564.2 XP_010811866.1 laminin subunit beta-3 isoform X1
XM_015475278.1 XP_015330764.1 laminin subunit beta-3 isoform X1

Pathways from BioSystems


Homologies


Gallus gallus (chicken) LAMB3 XP_425827.3
Xenopus tropicalis (tropical clawed frog) lamb3 XP_002933550.2
Homo sapiens (human) LAMB3 NP_001017402.1
Pan troglodytes (chimpanzee) LAMB3 XP_003949737.1
LAMB3 XP_002802197.1
Canis lupus familiaris (dog) LAMB3 XP_005622385.1
Bos taurus (cattle) LAMB3 NP_001075065.1
Rattus norvegicus (Norway rat) Lamb3 NP_001094311.1
Mus musculus (house mouse) Lamb3 NP_001264857.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LAMB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223617 XM_005217367.4
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_015475274.2
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_010813565.3
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_015475277.2
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa223617 XM_010813564.3
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OBa93058 XM_015475281.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $629.30
$899.00
OBa35997 NM_001081596.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_010813564.2
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475276.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475277.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475274.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_005217367.3
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475275.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475279.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475278.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_015475280.1
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00
OBa27520 XM_010813565.2
Latest version!		 Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa223617
Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
Accession Version XM_015475277.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330763.2
CDS312..3830
Translation

Target ORF information:

RefSeq Version XM_015475277.2
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475277.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa223617
Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
Accession Version XM_010813564.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813564.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010811866.2
CDS549..4067
Translation

Target ORF information:

RefSeq Version XM_010813564.3
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010813564.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa223617
Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
Accession Version XM_010813565.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813565.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010811867.2
CDS597..4115
Translation

Target ORF information:

RefSeq Version XM_010813565.3
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010813565.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa223617
Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
Accession Version XM_015475274.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330760.2
CDS1104..4622
Translation

Target ORF information:

RefSeq Version XM_015475274.2
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475274.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa223617
Clone ID Related Accession (Same CDS sequence) XM_015475277.2 , XM_010813564.3 , XM_010813565.3 , XM_015475274.2 , XM_005217367.4
Accession Version XM_005217367.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-06
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217367.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005217424.2
CDS554..4072
Translation

Target ORF information:

RefSeq Version XM_005217367.4
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005217367.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa93058
Clone ID Related Accession (Same CDS sequence) XM_015475281.1
Accession Version XM_015475281.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACGGACGG AATGGACAGA GGTGGGCTGG TGAGTCGACA 
GTGAGTGGAT TCGAGGCTGT GGCCAGCTGC ACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330767.1
CDS542..3997
Translation

Target ORF information:

RefSeq Version XM_015475281.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475281.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACGGACGG AATGGACAGA GGTGGGCTGG TGAGTCGACA 
GTGAGTGGAT TCGAGGCTGT GGCCAGCTGC ACCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa35997
Clone ID Related Accession (Same CDS sequence) NM_001081596.1
Accession Version NM_001081596.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-13
Organism Bos taurus(cattle)
Product laminin subunit beta-3 precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133303.1. On Feb 20, 2007 this sequence version replaced XM_608401.3. ##Evidence-Data-START## Transcript exon combination :: BC133303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCGGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001075065.1
CDS81..3599
Translation

Target ORF information:

RefSeq Version NM_001081596.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001081596.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCGGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACGGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCTGCC 
TCGCAGGTCC AGTCGGACAC TCAGCTCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCGGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475275.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330761.1
CDS848..4366
Translation

Target ORF information:

RefSeq Version XM_015475275.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475275.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475276.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330762.1
CDS1391..4909
Translation

Target ORF information:

RefSeq Version XM_015475276.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475276.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475277.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330763.1
CDS416..3934
Translation

Target ORF information:

RefSeq Version XM_015475277.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475277.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475280.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330766.1
CDS613..4131
Translation

Target ORF information:

RefSeq Version XM_015475280.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475280.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_010813565.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010811867.1
CDS1768..5286
Translation

Target ORF information:

RefSeq Version XM_010813565.2
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010813565.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475274.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330760.1
CDS1459..4977
Translation

Target ORF information:

RefSeq Version XM_015475274.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475274.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_005217367.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217367.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005217424.1
CDS165..3683
Translation

Target ORF information:

RefSeq Version XM_005217367.3
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005217367.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475279.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330765.1
CDS696..4214
Translation

Target ORF information:

RefSeq Version XM_015475279.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475279.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_010813564.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_010811866.1
CDS542..4060
Translation

Target ORF information:

RefSeq Version XM_010813564.2
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_010813564.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OBa27520
Clone ID Related Accession (Same CDS sequence) XM_015475275.1 , XM_015475276.1 , XM_015475277.1 , XM_015475280.1 , XM_010813565.2 , XM_015475274.1 , XM_005217367.3 , XM_015475279.1 , XM_010813564.2 , XM_015475278.1
Accession Version XM_015475278.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-01-25
Organism Bos taurus(cattle)
Product laminin subunit beta-3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_015330764.1
CDS445..3963
Translation

Target ORF information:

RefSeq Version XM_015475278.1
Organism Bos taurus(cattle)
Definition Bos taurus laminin subunit beta 3 (LAMB3), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_015475278.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGGCCGC TCCTTCTCCT GTATTTTGGC TTGCCCAGCC TCCTGTGTGC CCAGCAAGCA 
TGCTCCCGTG GGGCCTGCTA TCCACCCGTC GGGGACCTGC TCATAGGGAG GACCCGGTTT 
CTCCGAGCTT CATCCACCTG TGGCCTGACC AAGCCTGAGA CCTACTGTGC CCAATATGGC 
GAGTGGCAGA TGAAATGCTG CAAGTGTGAC TCCAGGGTGG CTCACAATTA CAACAGTCAC 
CAAGTGGAGA ACGTGGTTTC ATCCTCGGGC CCCACGCGCT GGTGGCAGTC ACAGAACGAT 
GTGAGCCCTG TCTTTCTGCA GCTGGACCTG GACAGGAAAT TCCAGCTTCA GGACATCATG 
ATGGATTTCA AGGGGCCCGT GCCTGCAGGC TTGCTGATCG AGCGCTCCTC GGACTTCGGC 
AAGACTTGGC AGGTGTACCA GTACCTGGCT GCTGACTGCG GCTCTGCCTT CCCCTGGGTC 
CGCCAGGGCC AACCCCAGGG CTGGCAGGAT GCTCGGTGCC AGACTCTGCC CCAGAGGCCT 
AATGGGCACC CGAACGGGGC CAAGGTCCAG CTCAACCTTA TGGATTTAGC CTCTGGGATC 
CCAGCCACAC AAAGTCAAAA AATTCAAGAG CTGGGGGAGA TCACCAACTT GAGGGTCAAC 
TTCACCAAGC TGGCGCCTGT GCCCCAGAGG GGCTACCACC CTCCCAGTGC CTACTACGCT 
GTGTCCCAGT TGCGTCTGCA TGGGAGCTGC CTCTGCCATG GCCATGCGGA CCGCTGCGCC 
CCCAGTTCCA GAACTCCTGC CAGCCCCTCC AGCGCCGTGC AGGTCCATGA GGTCTGTGTC 
TGCCAACACA ACACTGCCGG CCCCAACTGT GAGCGCTGCG CGCCCTTCTA CAACAACCGG 
CCCTGGAGAC CCGCGGAAGA CCAGGACCCC CACGAATGCC AGAGATGTGA CTGCAACGGA 
CACTCAGAGA CATGTCACTT TGACCCAGCC GTGTTTGCTG CCAGCCAGGG GGCGCACGGA 
GGTGTGTGTG ACAACTGCCG GGACCACACT GAGGGCAGGA ACTGTGAACG CTGTCAGCTG 
CACTACTTCC GGAACCGGCG TCCTGGGGCT CCCATTCAGG AGACCTGTAT CCCCTGTGAG 
TGTGATCCCG ACGGGGCAGT ACCTGGAGCA CCCTGTGACC CCCTGACTGG CCAGTGTGTG 
TGCAAGGAGC ACGTGCAGGG GGAACGCTGT GACCTGTGCA AGCCGGGGTT CACGGGGCTC 
ACCTTCTCCA ACCCGCAGGG CTGCCACCGC TGTGACTGCA GTGTCCTGGG CGCCCGGCGG 
GACGTGCCGT GTGACGAGGA GACTGGGCGC TGCGTGTGTC TGCCCCACGT GGTGGGCCCC 
AAGTGTGACC AATGTGCCCC CCACCACTGG AAGCTGGCCA GCGGCCGGGG CTGCGAGCCC 
TGTGCCTGCG ACCCGCACAA CTCCCTCGGC CCCCAGTGCA ACCAGTTCAC AGGGCAGTGC 
GCCTGTCGGG AAGGCTTCGG GGGCCTGACC TGCCGCGCCG CAGCCATCCG CCAGTGTCCC 
GACAGGACCT ATGGAGATGC AGCCGCAGGA TGCCGAGCCT GTGACTGCGA CTTCCGGGGC 
ACCGAGGGGC CAGGCTGCGA CAAGGCCTCG GGCCGCTGCC TCTGCCGCCC CGGCTTGACG 
GGGCCGCGCT GTGACCAATG CCAGCGGGGC TACTGCGACC GCGCCCCGGT GTGCGTGGCC 
TGCCACCCCT GCTTCCAGAC CTACGACGGC GGCCTCCGGG AGAGGGCCCT CCGCCTGGCC 
GGCCTCCGCA ATGCCTCCGC CGCCCTGTGG CCCCAGCCGG GCCTGGAGGA CCACGGTCTG 
GCCTCGCGGA TGCTGGACGC CAAGAGCAAG ATGGAGCAGA TCCAAGCCAC CCTCGGCGAT 
GCCTTGGTCA CGGAGGAGGA GGTGGCCCAG GTGGCCAATG CCATCTTCTC CATCAGGCGG 
ACGCTTCAGG GCCTACAGCT TGATTTGCCC CTGGAGGAGG AGACCTTGTC CCTCCCGGGA 
GACCTGGGGA GTCTGGACAG GAGCTTCAAT CGCCTCCTCG TCACATATCA GAGCAAGAGG 
GAGCAGTTTG AAAAGATAAG CAGTGCCGAC CCTTCAGGAG CCTTCCGCAT GCTGACTGCA 
GCCCATCAGC GGTCATCCCA GGCTGCTCAG CAGGTCTCGG ACAGCTCCCG CCGGTTGCTG 
CAGCTCAGGG ATAGCCGGAG AGAGGCGGAG AGGCTGGAGC AGCAGGTGGC AGGAGGACCA 
GGAGCCAGTG GTCCCCAGCT CGCGGCCCTG AGGCTGGAGA TGGCCTCCGT GCCTGATCTG 
ACGCCCACCA TCAACCAGCT CTGTGGGGGC TCCAGGGAGA TGCCTTGCAC CCCGGGAGCC 
TGCCCTGGGG AGCTCTGTCC CCGAGACAAC CGCACAGCCT GTGGCTCCCG CTGCAGAGGC 
GCCCTCCCCA GGGCAGGTGG GGCCTTGCGG ACGGCGGGGC AGGTGGCCGA GCAGCTGCGG 
GCCTTCAGCA CCCAGCTCCA GCAGACCAGG CAGATGATCA GGGCAGCGGA GGAGGCCGCC 
TCGCAGGTCC AGTCGGACAC TCAGCGCCTG GAGACGCAGG TGAGCGCCAG CCGCTCCCAG 
ATGGAGGAGG ACGTCAGACG CACGCGGCTC CTCATCCAGC AGGTGCGGGA GTTCCTCTCA 
GATCCCGACA CTGACACAGC CACCATCCAG GAGGTCAGCG AGGCTGTGCT GGCCCTGTGG 
CTGCCCACCG ACTCGGCCAC AGTCCTGCAG AAGATGAACG AGATCCAGGC CATTGCGGCC 
AGGCTCCCCA ACGTGGACCT GGTGCTGTCC CAGACCAAGC AGGATGTTGC TCGGGCTCGC 
AGGCTGCAGG CCGAGGCCGA GCAGGCCAGG AGCCGGGCCC ATGCGGTGGA GGGCCAGGTG 
GAAGAAGTCC TGGGGGCCCT GCGGCAGGGC ACGCTGGCGC TGCAGGAGGC CCAGGACACC 
ATGCGGGGCA CCCGCCGCTC CATGCGGCTC ATCCAGGACA GGGTCGCTGA GGTTCAGCAG 
GTACTGGGAC CAGCAGAAAG ACTGGTGACC AGCATGACGC AGCAGCTGGG GGGCTTCCGA 
GCACGGATGG AGGAGCTTGG CCGCCGGGCC AGGCAGCAGC AGGCACAGGC AGCCCAGGCC 
CGACAGCTGG CAGAGGAAGC CAGCAAGGAG GCGCTGAACG CGCAGGAGGG GTTTGAGAGA 
GTAAAGCAAA AATATGCTGA GTTAAAGGAC CGGTTGGATC AGAGCTCCAT GCTGGGAGAA 
CAGGGCAGCC GGATTCTGAG TGTCAAGACG GAGGCGGAGG AACTGTTTGG GGAGACCATG 
GAGATGATGG ACAGGATGAG AGACATGGAG TCAGAGCTGC TTCAGGGGAG CCAGGCCATC 
GTGCTCCGCT CGGCGGACCT GACGGGGCTG GAGCAGCGCG TGGAACAGAT TCGGAACCAC 
ATCAACGGGC GCGTGCTCTA CTACGCCACC TGCAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL