DAGLB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Gene Symbol	DAGLB
Entrez Gene ID	538021
Full Name	diacylglycerol lipase beta
General protein information	Preferred Names diacylglycerol lipase beta Names sn1-specific diacylglycerol lipase beta
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015460486.2	XP_015315972.1	sn1-specific diacylglycerol lipase beta isoform X2
XM_024985005.1	XP_024840773.1	sn1-specific diacylglycerol lipase beta isoform X3
XM_024985004.1	XP_024840772.1	sn1-specific diacylglycerol lipase beta isoform X1
NM_001083487.1	NP_001076956.1	sn1-specific diacylglycerol lipase beta
XM_015460486.1	XP_015315972.1	sn1-specific diacylglycerol lipase beta isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC100612262	XP_003318317.1
Rattus norvegicus (Norway rat)	Daglb	NP_001100590.1
Danio rerio (zebrafish)	daglb	XP_693659.5
Homo sapiens (human)	DAGLB	NP_631918.3
Canis lupus familiaris (dog)	DAGLB	XP_536885.2
Gallus gallus (chicken)	DAGLB	NP_001025814.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012171	XP_320362.4
Xenopus tropicalis (tropical clawed frog)	daglb	XP_002931999.1
Bos taurus (cattle)	DAGLB	NP_001076956.1
Mus musculus (house mouse)	Daglb	NP_659164.2
Caenorhabditis elegans (roundworm)	F42G9.6	NP_741085.1
Arabidopsis thaliana (thale cress)	AT1G05790	NP_001077463.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DAGLB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAGLB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230373	XM_024985004.1 Latest version!	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa230374	XM_024985005.1 Latest version!	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa37353	NM_001083487.1 Latest version!	Bos taurus diacylglycerol lipase beta (DAGLB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBa88577	XM_015460486.2 Latest version!	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OBa88577	XM_015460486.1	Bos taurus diacylglycerol lipase, beta (DAGLB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAGLB ( XM_024985004.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840772.1 Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230373
Clone ID Related Accession (Same CDS sequence)	XM_024985004.1
Accession Version	XM_024985004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGA TGGTGCTGTT CGGCCGGCGT TGGGCCATCG CCAGCGACGA CTTGGTCTTC 
CCGGGGGTCT TCGAGCTGTT GCTGCGAGTG CTGTGGTGGG TTGGCATCCT GATGCTGTAC 
CTCATGCACA GAGGGAAGCT GGACTGCCCG GGGGGCGTCC TGCTCAGCAG CTACCTGATC 
GTGCTCCTCG TCCTCCTGGC GATTATCATA GGCACAGTGT CAGCCATCGT GTGTGTCAGC 
ATGAAAGGGA CCATCTGTAA TCCAGGACCG CGGAAATCCA TGTCCAAGCT GCTGTACGTA 
CGCCTGGCGC TCTTCCTGCC AGAGATGGTC TGGGCATCCC TGGGGGCCGC CTGGATCGCA 
GATGGCATTC AGTGTGACCG GACAGTGGGC AACGGCATCA TCGCGACCGT CGTGGTCAGC 
TGGCTCATCA TCGCCTCCAC CGTGCTCACC ATCCTCATCG TCTTCGACCC GCTGGGGGGC 
AAGGCGGCCC CGGGCGCCCC CCTGGGCCCC CAGCCCCTGG ACAGCCGAGA GTCCAGCCAG 
CTGCTGAGTG GCCTCAGGAC GGCGGCGACC GGCGTGTGGG AGACGCGAGT CAGGCTGCTG 
TGCTGCTGCA TCGGCCGGGA CGACCACACT CGCGTCGCCT TTTCGAGTAC GGCAGAACTT 
TTCTCAACCT ACTTTTCAGA CACGGACCTG GTGCCCAGCG ACATTGCGGC CGGCCTCGCC 
CTTCTCCATC AGCAGCAGGA CCATGCACGG AGCCCCCCGG AGCCCACCGA GGTAGTCACC 
CACTCCCCCG GGCCCTTCCA GGAGGCCGAT CTGGACGCAG AGCTGGAGAA CTGCCACCAC 
TACATGCAGT TCGCAGCCGC CGCCTACGGG TGGCCCCTCT ACGTCTACAG GAACCCCTTC 
ACGGGGCTCT GCCGGGTCGG CGGAGACTGC TGCAGATGCA GGACCACCGA CTACGATTTG 
GTTGGAGGCG ATCAGCTCAA CTGCCACTTC AGCTCCATCC TGCAGACGAC GGGGCTGCAG 
CACCGAGACT TCATCCACAT CAGCTTCCAC GACAAGGTGT ACGAGCTCCC CTTCTTGGTG 
GCCCTGGACC ATAGGAAGGA GTCAGTGGTG GTGGCCATCA GAGGGACCAT GTCTCTGCAG 
GACATCCTCA CGGACCTGTC TGCGGAGAGC GAGCCCCTCG ACATTGAGTG TGAGGCGCAG 
GACTGCTGGG CACACAAGGA GTACCGGCTG GTCGTCGTGG GGCACAGCCT GGGCGCGGGC 
GCCGCGGCGC TGCTGGCCCT CATGCTCAAG AGCCCGTACC CGCAGGTCAG GTGCTACGCC 
TTCTCCCCGC CCAGGGGGCT GCTCAGCAAA TCCCTTTATG AATACTCCAA GACCTTCATC 
GTGTCCCTTG TCCTGGGCAA GGACGTGATT CCCAGGTTGA GTGTAACCAA CCTGGAAGAT 
CTGAAGCGGA GGATCCTGCG AGTGATCGCC CACTGCAACA AGCCCAAGTA CAAGATCCTG 
CTGCGCGGGT GCTGGTATGA GCTGTTCGGA GGGAGCCCCG AGGACCTTCC CACCGAGCTG 
GATGGAGGCG ACCTGACGCA GCCCCTCCTC GGGGAGCACA GCCTGCTGGT GCACGGGTCC 
CCGGCCTACA GCTTCTCCAG CGAGTCCCCG CTCGAGTCGC CCACCAAGTA CCCGCCACTC 
TACCCTCCCG GCAGGATCAT CCACCTGGAG GAAGAAGGCA CCTCAGGGAG CTGCTCCTGC 
TCCCCTGCCG CTCGGTACAC CGTGAGGTGG GCCCACGAGT CAGAATTCAG CAGAATACTC 
ATCGGCCCCA AGATGCTCAC AGACCACATG CCAGACATCC TGATGAGGGT CCTGGACCAC 
GTGGTGTCCG ACAGGGCTGC CTGCGTTTCC TGCCCGGCCC AGGGAGGCTC CAGCGTGGAC 
CAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840772.1
CDS	81..2009
Translation	MPGMVLFGRRWAIASDDLVFPGVFELLLRVLWWVGILMLYLMHRGKLDCPGGVLLSSYLIVLLVLLAIIIGTVSAIVCVSMKGTICNPGPRKSMSKLLYVRLALFLPEMVWASLGAAWIADGIQCDRTVGNGIIATVVVSWLIIASTVLTILIVFDPLGGKAAPGAPLGPQPLDSRESSQLLSGLRTAATGVWETRVRLLCCCIGRDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDHARSPPEPTEVVTHSPGPFQEADLDAELENCHHYMQFAAAAYGWPLYVYRNPFTGLCRVGGDCCRCRTTDYDLVGGDQLNCHFSSILQTTGLQHRDFIHISFHDKVYELPFLVALDHRKESVVVAIRGTMSLQDILTDLSAESEPLDIECEAQDCWAHKEYRLVVVGHSLGAGAAALLALMLKSPYPQVRCYAFSPPRGLLSKSLYEYSKTFIVSLVLGKDVIPRLSVTNLEDLKRRILRVIAHCNKPKYKILLRGCWYELFGGSPEDLPTELDGGDLTQPLLGEHSLLVHGSPAYSFSSESPLESPTKYPPLYPPGRIIHLEEEGTSGSCSCSPAARYTVRWAHESEFSRILIGPKMLTDHMPDILMRVLDHVVSDRAACVSCPAQGGSSVDQA

Target ORF information:

RefSeq Version	XM_024985004.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985004.1

ORF Insert Sequence:

ATGCCGGGGA TGGTGCTGTT CGGCCGGCGT TGGGCCATCG CCAGCGACGA CTTGGTCTTC 
CCGGGGGTCT TCGAGCTGTT GCTGCGAGTG CTGTGGTGGG TTGGCATCCT GATGCTGTAC 
CTCATGCACA GAGGGAAGCT GGACTGCCCG GGGGGCGTCC TGCTCAGCAG CTACCTGATC 
GTGCTCCTCG TCCTCCTGGC GATTATCATA GGCACAGTGT CAGCCATCGT GTGTGTCAGC 
ATGAAAGGGA CCATCTGTAA TCCAGGACCG CGGAAATCCA TGTCCAAGCT GCTGTACGTA 
CGCCTGGCGC TCTTCCTGCC AGAGATGGTC TGGGCATCCC TGGGGGCCGC CTGGATCGCA 
GATGGCATTC AGTGTGACCG GACAGTGGGC AACGGCATCA TCGCGACCGT CGTGGTCAGC 
TGGCTCATCA TCGCCTCCAC CGTGCTCACC ATCCTCATCG TCTTCGACCC GCTGGGGGGC 
AAGGCGGCCC CGGGCGCCCC CCTGGGCCCC CAGCCCCTGG ACAGCCGAGA GTCCAGCCAG 
CTGCTGAGTG GCCTCAGGAC GGCGGCGACC GGCGTGTGGG AGACGCGAGT CAGGCTGCTG 
TGCTGCTGCA TCGGCCGGGA CGACCACACT CGCGTCGCCT TTTCGAGTAC GGCAGAACTT 
TTCTCAACCT ACTTTTCAGA CACGGACCTG GTGCCCAGCG ACATTGCGGC CGGCCTCGCC 
CTTCTCCATC AGCAGCAGGA CCATGCACGG AGCCCCCCGG AGCCCACCGA GGTAGTCACC 
CACTCCCCCG GGCCCTTCCA GGAGGCCGAT CTGGACGCAG AGCTGGAGAA CTGCCACCAC 
TACATGCAGT TCGCAGCCGC CGCCTACGGG TGGCCCCTCT ACGTCTACAG GAACCCCTTC 
ACGGGGCTCT GCCGGGTCGG CGGAGACTGC TGCAGATGCA GGACCACCGA CTACGATTTG 
GTTGGAGGCG ATCAGCTCAA CTGCCACTTC AGCTCCATCC TGCAGACGAC GGGGCTGCAG 
CACCGAGACT TCATCCACAT CAGCTTCCAC GACAAGGTGT ACGAGCTCCC CTTCTTGGTG 
GCCCTGGACC ATAGGAAGGA GTCAGTGGTG GTGGCCATCA GAGGGACCAT GTCTCTGCAG 
GACATCCTCA CGGACCTGTC TGCGGAGAGC GAGCCCCTCG ACATTGAGTG TGAGGCGCAG 
GACTGCTGGG CACACAAGGA GTACCGGCTG GTCGTCGTGG GGCACAGCCT GGGCGCGGGC 
GCCGCGGCGC TGCTGGCCCT CATGCTCAAG AGCCCGTACC CGCAGGTCAG GTGCTACGCC 
TTCTCCCCGC CCAGGGGGCT GCTCAGCAAA TCCCTTTATG AATACTCCAA GACCTTCATC 
GTGTCCCTTG TCCTGGGCAA GGACGTGATT CCCAGGTTGA GTGTAACCAA CCTGGAAGAT 
CTGAAGCGGA GGATCCTGCG AGTGATCGCC CACTGCAACA AGCCCAAGTA CAAGATCCTG 
CTGCGCGGGT GCTGGTATGA GCTGTTCGGA GGGAGCCCCG AGGACCTTCC CACCGAGCTG 
GATGGAGGCG ACCTGACGCA GCCCCTCCTC GGGGAGCACA GCCTGCTGGT GCACGGGTCC 
CCGGCCTACA GCTTCTCCAG CGAGTCCCCG CTCGAGTCGC CCACCAAGTA CCCGCCACTC 
TACCCTCCCG GCAGGATCAT CCACCTGGAG GAAGAAGGCA CCTCAGGGAG CTGCTCCTGC 
TCCCCTGCCG CTCGGTACAC CGTGAGGTGG GCCCACGAGT CAGAATTCAG CAGAATACTC 
ATCGGCCCCA AGATGCTCAC AGACCACATG CCAGACATCC TGATGAGGGT CCTGGACCAC 
GTGGTGTCCG ACAGGGCTGC CTGCGTTTCC TGCCCGGCCC AGGGAGGCTC CAGCGTGGAC 
CAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLB ( XM_024985005.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840773.1 Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa230374
Clone ID Related Accession (Same CDS sequence)	XM_024985005.1
Accession Version	XM_024985005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC TGCTGTACGT ACGCCTGGCG CTCTTCCTGC CAGAGATGGT CTGGGCATCC 
CTGGGGGCCG CCTGGATCGC AGATGGCATT CAGTGTGACC GGACAGTGGG CAACGGCATC 
ATCGCGACCG TCGTGGTCAG CTGGCTCATC ATCGCCTCCA CCGTGCTCAC CATCCTCATC 
GTCTTCGACC CGCTGGGGGG CAAGGCGGCC CCGGGCGCCC CCCTGGGCCC CCAGCCCCTG 
GACAGCCGAG AGTCCAGCCA GCTGCTGAGT GGCCTCAGGA CGGCGGCGAC CGGCGTGTGG 
GAGACGCGAG TCAGGCTGCT GTGCTGCTGC ATCGGCCGGG ACGACCACAC TCGCGTCGCC 
TTTTCGAGTA CGGCAGAACT TTTCTCAACC TACTTTTCAG ACACGGACCT GGTGCCCAGC 
GACATTGCGG CCGGCCTCGC CCTTCTCCAT CAGCAGCAGG ACCATGCACG GAGCCCCCCG 
GAGCCCACCG AGGTAGTCAC CCACTCCCCC GGGCCCTTCC AGGAGGCCGA TCTGGACGCA 
GAGCTGGAGA ACTGCCACCA CTACATGCAG TTCGCAGCCG CCGCCTACGG GTGGCCCCTC 
TACGTCTACA GGAACCCCTT CACGGGGCTC TGCCGGGTCG GCGGAGACTG CTGCAGATGC 
AGGACCACCG ACTACGATTT GGTTGGAGGC GATCAGCTCA ACTGCCACTT CAGCTCCATC 
CTGCAGACGA CGGGGCTGCA GCACCGAGAC TTCATCCACA TCAGCTTCCA CGACAAGGTG 
TACGAGCTCC CCTTCTTGGT GGCCCTGGAC CATAGGAAGG AGTCAGTGGT GGTGGCCATC 
AGAGGGACCA TGTCTCTGCA GGACATCCTC ACGGACCTGT CTGCGGAGAG CGAGCCCCTC 
GACATTGAGT GTGAGGCGCA GGACTGCTGG GCACACAAGG GCATCTCTCA GGCCGCCAGA 
TACGTCTACC GCCGACTCGT CAACGACGGG ATTCTGAGCC AAGCGTTCAG CATTGCACCT 
GAGTACCGGC TGGTCGTCGT GGGGCACAGC CTGGGCGCGG GCGCCGCGGC GCTGCTGGCC 
CTCATGCTCA AGAGCCCGTA CCCGCAGGTC AGGTGCTACG CCTTCTCCCC GCCCAGGGGG 
CTGCTCAGCA AATCCCTTTA TGAATACTCC AAGACCTTCA TCGTGTCCCT TGTCCTGGGC 
AAGGACGTGA TTCCCAGGTT GAGTGTAACC AACCTGGAAG ATCTGAAGCG GAGGATCCTG 
CGAGTGATCG CCCACTGCAA CAAGCCCAAG TACAAGATCC TGCTGCGCGG GTGCTGGTAT 
GAGCTGTTCG GAGGGAGCCC CGAGGACCTT CCCACCGAGC TGGATGGAGG CGACCTGACG 
CAGCCCCTCC TCGGGGAGCA CAGCCTGCTG GTGCACGGGT CCCCGGCCTA CAGCTTCTCC 
AGCGAGTCCC CGCTCGAGTC GCCCACCAAG TACCCGCCAC TCTACCCTCC CGGCAGGATC 
ATCCACCTGG AGGAAGAAGG CACCTCAGGG AGCTGCTCCT GCTCCCCTGC CGCTCGGTAC 
ACCGTGAGGT GGGCCCACGA GTCAGAATTC AGCAGAATAC TCATCGGCCC CAAGATGCTC 
ACAGACCACA TGCCAGACAT CCTGATGAGG GTCCTGGACC ACGTGGTGTC CGACAGGGCT 
GCCTGCGTTT CCTGCCCGGC CCAGGGAGGC TCCAGCGTGG ACCAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840773.1
CDS	171..1901
Translation	MSKLLYVRLALFLPEMVWASLGAAWIADGIQCDRTVGNGIIATVVVSWLIIASTVLTILIVFDPLGGKAAPGAPLGPQPLDSRESSQLLSGLRTAATGVWETRVRLLCCCIGRDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDHARSPPEPTEVVTHSPGPFQEADLDAELENCHHYMQFAAAAYGWPLYVYRNPFTGLCRVGGDCCRCRTTDYDLVGGDQLNCHFSSILQTTGLQHRDFIHISFHDKVYELPFLVALDHRKESVVVAIRGTMSLQDILTDLSAESEPLDIECEAQDCWAHKGISQAARYVYRRLVNDGILSQAFSIAPEYRLVVVGHSLGAGAAALLALMLKSPYPQVRCYAFSPPRGLLSKSLYEYSKTFIVSLVLGKDVIPRLSVTNLEDLKRRILRVIAHCNKPKYKILLRGCWYELFGGSPEDLPTELDGGDLTQPLLGEHSLLVHGSPAYSFSSESPLESPTKYPPLYPPGRIIHLEEEGTSGSCSCSPAARYTVRWAHESEFSRILIGPKMLTDHMPDILMRVLDHVVSDRAACVSCPAQGGSSVDQA

Target ORF information:

RefSeq Version	XM_024985005.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985005.1

ORF Insert Sequence:

ATGTCCAAGC TGCTGTACGT ACGCCTGGCG CTCTTCCTGC CAGAGATGGT CTGGGCATCC 
CTGGGGGCCG CCTGGATCGC AGATGGCATT CAGTGTGACC GGACAGTGGG CAACGGCATC 
ATCGCGACCG TCGTGGTCAG CTGGCTCATC ATCGCCTCCA CCGTGCTCAC CATCCTCATC 
GTCTTCGACC CGCTGGGGGG CAAGGCGGCC CCGGGCGCCC CCCTGGGCCC CCAGCCCCTG 
GACAGCCGAG AGTCCAGCCA GCTGCTGAGT GGCCTCAGGA CGGCGGCGAC CGGCGTGTGG 
GAGACGCGAG TCAGGCTGCT GTGCTGCTGC ATCGGCCGGG ACGACCACAC TCGCGTCGCC 
TTTTCGAGTA CGGCAGAACT TTTCTCAACC TACTTTTCAG ACACGGACCT GGTGCCCAGC 
GACATTGCGG CCGGCCTCGC CCTTCTCCAT CAGCAGCAGG ACCATGCACG GAGCCCCCCG 
GAGCCCACCG AGGTAGTCAC CCACTCCCCC GGGCCCTTCC AGGAGGCCGA TCTGGACGCA 
GAGCTGGAGA ACTGCCACCA CTACATGCAG TTCGCAGCCG CCGCCTACGG GTGGCCCCTC 
TACGTCTACA GGAACCCCTT CACGGGGCTC TGCCGGGTCG GCGGAGACTG CTGCAGATGC 
AGGACCACCG ACTACGATTT GGTTGGAGGC GATCAGCTCA ACTGCCACTT CAGCTCCATC 
CTGCAGACGA CGGGGCTGCA GCACCGAGAC TTCATCCACA TCAGCTTCCA CGACAAGGTG 
TACGAGCTCC CCTTCTTGGT GGCCCTGGAC CATAGGAAGG AGTCAGTGGT GGTGGCCATC 
AGAGGGACCA TGTCTCTGCA GGACATCCTC ACGGACCTGT CTGCGGAGAG CGAGCCCCTC 
GACATTGAGT GTGAGGCGCA GGACTGCTGG GCACACAAGG GCATCTCTCA GGCCGCCAGA 
TACGTCTACC GCCGACTCGT CAACGACGGG ATTCTGAGCC AAGCGTTCAG CATTGCACCT 
GAGTACCGGC TGGTCGTCGT GGGGCACAGC CTGGGCGCGG GCGCCGCGGC GCTGCTGGCC 
CTCATGCTCA AGAGCCCGTA CCCGCAGGTC AGGTGCTACG CCTTCTCCCC GCCCAGGGGG 
CTGCTCAGCA AATCCCTTTA TGAATACTCC AAGACCTTCA TCGTGTCCCT TGTCCTGGGC 
AAGGACGTGA TTCCCAGGTT GAGTGTAACC AACCTGGAAG ATCTGAAGCG GAGGATCCTG 
CGAGTGATCG CCCACTGCAA CAAGCCCAAG TACAAGATCC TGCTGCGCGG GTGCTGGTAT 
GAGCTGTTCG GAGGGAGCCC CGAGGACCTT CCCACCGAGC TGGATGGAGG CGACCTGACG 
CAGCCCCTCC TCGGGGAGCA CAGCCTGCTG GTGCACGGGT CCCCGGCCTA CAGCTTCTCC 
AGCGAGTCCC CGCTCGAGTC GCCCACCAAG TACCCGCCAC TCTACCCTCC CGGCAGGATC 
ATCCACCTGG AGGAAGAAGG CACCTCAGGG AGCTGCTCCT GCTCCCCTGC CGCTCGGTAC 
ACCGTGAGGT GGGCCCACGA GTCAGAATTC AGCAGAATAC TCATCGGCCC CAAGATGCTC 
ACAGACCACA TGCCAGACAT CCTGATGAGG GTCCTGGACC ACGTGGTGTC CGACAGGGCT 
GCCTGCGTTT CCTGCCCGGC CCAGGGAGGC TCCAGCGTGG ACCAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLB ( NM_001083487.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001076956.1 Bos taurus diacylglycerol lipase beta (DAGLB), mRNA.

Price & Availability↑

CloneID	OBa37353
Clone ID Related Accession (Same CDS sequence)	NM_001083487.1
Accession Version	NM_001083487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133292.1. On Mar 27, 2007 this sequence version replaced XM_618212.3. ##Evidence-Data-START## Transcript exon combination :: BC133292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGGGA TGGTGCTGTT CGGCCGGCGT TGGGCCATCG CCAGCGACGA CTTGGTCTTC 
CCGGGGGTCT TCGAGCTGTT GCTGCGAGTG CTGTGGTGGG TTGGCATCCT GATGCTGTAC 
CTCATGCACA GAGGGAAGCT GGACTGCCCG GGGGGCGTCC TGCTCAGCAG CTACCTGATC 
GTGCTCCTCG TCCTCCTGGC GATTATCATA GGCACAGTGT CAGCCATCGT GTGTGTCAGC 
ATGAAAGGGA CCATCTGTAA TCCAGGACCG CGGAAATCCA TGTCCAAGCT GCTGTACGTA 
CGCCTGGCGC TCTTCCTGCC AGAGATGGTC TGGGCATCCC TGGGGGCCGC CTGGATCGCA 
GATGGCATTC AGTGTGACCG GACAGTGGGC AACGGCATCA TCGCGACCGT CGTGGTCAGC 
TGGCTCATCA TCGCCTCCAC CGTGCTCACC ATCCTCATCG TCTTCGACCC GCTGGGGGGC 
AAGGCGGCCC CGGGCGCCCC CCTGGGCCCC CAGCCCCTGG ACAGCCGAGA GTCCAGCCAG 
CTGCTGAGTG GCCTCAGGAC GGCGGCGACC GGCGTGTGGG AGACGCGAGT CAGGCTGCTG 
TGCTGCTGCA TCGGCCGGGA CGACCACACT CGCGTCGCCT TTTCGAGTAC GGCAGAACTT 
TTCTCAACCT ACTTTTCAGA CACGGACCTG GTGCCCAGCG ACATTGCGGC CGGCCTCGCC 
CTTCTCCATC AGCAGCAGGA CCATGCACGG AGCCCCCCGG AGCCCACCGA GGTAGTCACC 
CACTCCCCCG GGCCCTTCCA GGAGGCCGAT CTGGACGCAG AGCTGGAGAA CTGCCACCAC 
TACATGCAGT TCGCAGCCGC CGCCTACGGG TGGCCCCTCT ACGTCTACAG GAACCCCTTC 
ACGGGGCTCT GCCGGGTCGG CGGAGACTGC TGCAGATGCA GGACCACCGA CTACGATTTG 
GTTGGAGGCG ATCAGCTCAA CTGCCACTTC AGCTCCATCC TGCAGACGAC GGGGCTGCAG 
CACCGAGACT TCATCCACAT CAGCTTCCAC GACAAGGTGT ACGAGCTCCC CTTCTTGGTG 
GCCCTGGACC ATAGGAAGGA GTCAGTGGTG GTGGCCATCA GAGGGACCAT GTCTCTGCAG 
GACATCCTCA CGGACCTGTC TGCGGAGAGC GAGCCCCTCG ACATTGAGTG TGAGGCGCAG 
GACTGCTGGG CACACAAGGG CATCTCTCAG GCCGCCAGAT ACGTCTACCG CCGACTCGTC 
AACGACGGGA TTCTGAGCCA AGCGTTCAGC ATTGCACCTG AGTACCGGCT GGTCGTCGTG 
GGGCACAGCC TGGGCGCGGG CGCCGCGGCG CTGCTGGCCC TCATGCTCAA GAGCCCGTAC 
CCGCAGGTCA GGTGCTACGC CTTCTCCCCG CCCAGGGGGC TGCTCAGCAA ATCCCTTTAT 
GAATACTCCA AGACCTTCAT CGTGTCCCTT GTCCTGGGCA AGGACGTGAT TCCCAGGTTG 
AGTGTAACCA ACCTGGAAGA TCTGAAGCGG AGGATCCTGC GAGTGATCGC CCACTGCAAC 
AAGCCCAAGT ACAAGATCCT GCTGCGCGGG TGCTGGTATG AGCTGTTCGG AGGGAGCCCC 
GAGGACCTTC CCACCGAGCT GGATGGAGGC GACCTGACGC AGCCCCTCCT CGGGGAGCAC 
AGCCTGCTGG TGCACGGGTC CCCGGCCTAC AGCTTCTCCA GCGAGTCCCC GCTCGAGTCG 
CCCACCAAGT ACCCGCCACT CTACCCTCCC GGCAGGATCA TCCACCTGGA GGAAGAAGGC 
ACCTCAGGGA GCTGCTCCTG CTCCCCTGCC GCTCGGTACA CCGTGAGGTG GGCCCACGAG 
TCAGAATTCA GCAGAATACT CATCGGCCCC AAGATGCTCA CAGACCACAT GCCAGACATC 
CTGATGAGGG TCCTGGACCA CGTGGTGTCC GACAGGGCTG CCTGCGTTTC CTGCCCGGCC 
CAGGGAGGCT CCAGCGTGGA CCAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076956.1
CDS	25..2034
Translation	MPGMVLFGRRWAIASDDLVFPGVFELLLRVLWWVGILMLYLMHRGKLDCPGGVLLSSYLIVLLVLLAIIIGTVSAIVCVSMKGTICNPGPRKSMSKLLYVRLALFLPEMVWASLGAAWIADGIQCDRTVGNGIIATVVVSWLIIASTVLTILIVFDPLGGKAAPGAPLGPQPLDSRESSQLLSGLRTAATGVWETRVRLLCCCIGRDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDHARSPPEPTEVVTHSPGPFQEADLDAELENCHHYMQFAAAAYGWPLYVYRNPFTGLCRVGGDCCRCRTTDYDLVGGDQLNCHFSSILQTTGLQHRDFIHISFHDKVYELPFLVALDHRKESVVVAIRGTMSLQDILTDLSAESEPLDIECEAQDCWAHKGISQAARYVYRRLVNDGILSQAFSIAPEYRLVVVGHSLGAGAAALLALMLKSPYPQVRCYAFSPPRGLLSKSLYEYSKTFIVSLVLGKDVIPRLSVTNLEDLKRRILRVIAHCNKPKYKILLRGCWYELFGGSPEDLPTELDGGDLTQPLLGEHSLLVHGSPAYSFSSESPLESPTKYPPLYPPGRIIHLEEEGTSGSCSCSPAARYTVRWAHESEFSRILIGPKMLTDHMPDILMRVLDHVVSDRAACVSCPAQGGSSVDQA

Target ORF information:

RefSeq Version	NM_001083487.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase beta (DAGLB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083487.1

ORF Insert Sequence:

ATGCCGGGGA TGGTGCTGTT CGGCCGGCGT TGGGCCATCG CCAGCGACGA CTTGGTCTTC 
CCGGGGGTCT TCGAGCTGTT GCTGCGAGTG CTGTGGTGGG TTGGCATCCT GATGCTGTAC 
CTCATGCACA GAGGGAAGCT GGACTGCCCG GGGGGCGTCC TGCTCAGCAG CTACCTGATC 
GTGCTCCTCG TCCTCCTGGC GATTATCATA GGCACAGTGT CAGCCATCGT GTGTGTCAGC 
ATGAAAGGGA CCATCTGTAA TCCAGGACCG CGGAAATCCA TGTCCAAGCT GCTGTACGTA 
CGCCTGGCGC TCTTCCTGCC AGAGATGGTC TGGGCATCCC TGGGGGCCGC CTGGATCGCA 
GATGGCATTC AGTGTGACCG GACAGTGGGC AACGGCATCA TCGCGACCGT CGTGGTCAGC 
TGGCTCATCA TCGCCTCCAC CGTGCTCACC ATCCTCATCG TCTTCGACCC GCTGGGGGGC 
AAGGCGGCCC CGGGCGCCCC CCTGGGCCCC CAGCCCCTGG ACAGCCGAGA GTCCAGCCAG 
CTGCTGAGTG GCCTCAGGAC GGCGGCGACC GGCGTGTGGG AGACGCGAGT CAGGCTGCTG 
TGCTGCTGCA TCGGCCGGGA CGACCACACT CGCGTCGCCT TTTCGAGTAC GGCAGAACTT 
TTCTCAACCT ACTTTTCAGA CACGGACCTG GTGCCCAGCG ACATTGCGGC CGGCCTCGCC 
CTTCTCCATC AGCAGCAGGA CCATGCACGG AGCCCCCCGG AGCCCACCGA GGTAGTCACC 
CACTCCCCCG GGCCCTTCCA GGAGGCCGAT CTGGACGCAG AGCTGGAGAA CTGCCACCAC 
TACATGCAGT TCGCAGCCGC CGCCTACGGG TGGCCCCTCT ACGTCTACAG GAACCCCTTC 
ACGGGGCTCT GCCGGGTCGG CGGAGACTGC TGCAGATGCA GGACCACCGA CTACGATTTG 
GTTGGAGGCG ATCAGCTCAA CTGCCACTTC AGCTCCATCC TGCAGACGAC GGGGCTGCAG 
CACCGAGACT TCATCCACAT CAGCTTCCAC GACAAGGTGT ACGAGCTCCC CTTCTTGGTG 
GCCCTGGACC ATAGGAAGGA GTCAGTGGTG GTGGCCATCA GAGGGACCAT GTCTCTGCAG 
GACATCCTCA CGGACCTGTC TGCGGAGAGC GAGCCCCTCG ACATTGAGTG TGAGGCGCAG 
GACTGCTGGG CACACAAGGG CATCTCTCAG GCCGCCAGAT ACGTCTACCG CCGACTCGTC 
AACGACGGGA TTCTGAGCCA AGCGTTCAGC ATTGCACCTG AGTACCGGCT GGTCGTCGTG 
GGGCACAGCC TGGGCGCGGG CGCCGCGGCG CTGCTGGCCC TCATGCTCAA GAGCCCGTAC 
CCGCAGGTCA GGTGCTACGC CTTCTCCCCG CCCAGGGGGC TGCTCAGCAA ATCCCTTTAT 
GAATACTCCA AGACCTTCAT CGTGTCCCTT GTCCTGGGCA AGGACGTGAT TCCCAGGTTG 
AGTGTAACCA ACCTGGAAGA TCTGAAGCGG AGGATCCTGC GAGTGATCGC CCACTGCAAC 
AAGCCCAAGT ACAAGATCCT GCTGCGCGGG TGCTGGTATG AGCTGTTCGG AGGGAGCCCC 
GAGGACCTTC CCACCGAGCT GGATGGAGGC GACCTGACGC AGCCCCTCCT CGGGGAGCAC 
AGCCTGCTGG TGCACGGGTC CCCGGCCTAC AGCTTCTCCA GCGAGTCCCC GCTCGAGTCG 
CCCACCAAGT ACCCGCCACT CTACCCTCCC GGCAGGATCA TCCACCTGGA GGAAGAAGGC 
ACCTCAGGGA GCTGCTCCTG CTCCCCTGCC GCTCGGTACA CCGTGAGGTG GGCCCACGAG 
TCAGAATTCA GCAGAATACT CATCGGCCCC AAGATGCTCA CAGACCACAT GCCAGACATC 
CTGATGAGGG TCCTGGACCA CGTGGTGTCC GACAGGGCTG CCTGCGTTTC CTGCCCGGCC 
CAGGGAGGCT CCAGCGTGGA CCAGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLB ( XM_015460486.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315972.1 Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa88577
Clone ID Related Accession (Same CDS sequence)	XM_015460486.2 , XM_015460486.1
Accession Version	XM_015460486.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAA CTGTGTTACA GGGACTTCCC TGGTGGGTTG GCATCCTGAT GCTGTACCTC 
ATGCACAGAG GGAAGCTGGA CTGCCCGGGG GGCGTCCTGC TCAGCAGCTA CCTGATCGTG 
CTCCTCGTCC TCCTGGCGAT TATCATAGGC ACAGTGTCAG CCATCGTGTG TGTCAGCATG 
AAAGGGACCA TCTGTAATCC AGGACCGCGG AAATCCATGT CCAAGCTGCT GTACGTACGC 
CTGGCGCTCT TCCTGCCAGA GATGGTCTGG GCATCCCTGG GGGCCGCCTG GATCGCAGAT 
GGCATTCAGT GTGACCGGAC AGTGGGCAAC GGCATCATCG CGACCGTCGT GGTCAGCTGG 
CTCATCATCG CCTCCACCGT GCTCACCATC CTCATCGTCT TCGACCCGCT GGGGGGCAAG 
GCGGCCCCGG GCGCCCCCCT GGGCCCCCAG CCCCTGGACA GCCGAGAGTC CAGCCAGCTG 
CTGAGTGGCC TCAGGACGGC GGCGACCGGC GTGTGGGAGA CGCGAGTCAG GCTGCTGTGC 
TGCTGCATCG GCCGGGACGA CCACACTCGC GTCGCCTTTT CGAGTACGGC AGAACTTTTC 
TCAACCTACT TTTCAGACAC GGACCTGGTG CCCAGCGACA TTGCGGCCGG CCTCGCCCTT 
CTCCATCAGC AGCAGGACCA TGCACGGAGC CCCCCGGAGC CCACCGAGGT AGTCACCCAC 
TCCCCCGGGC CCTTCCAGGA GGCCGATCTG GACGCAGAGC TGGAGAACTG CCACCACTAC 
ATGCAGTTCG CAGCCGCCGC CTACGGGTGG CCCCTCTACG TCTACAGGAA CCCCTTCACG 
GGGCTCTGCC GGGTCGGCGG AGACTGCTGC AGATGCAGGA CCACCGACTA CGATTTGGTT 
GGAGGCGATC AGCTCAACTG CCACTTCAGC TCCATCCTGC AGACGACGGG GCTGCAGCAC 
CGAGACTTCA TCCACATCAG CTTCCACGAC AAGGTGTACG AGCTCCCCTT CTTGGTGGCC 
CTGGACCATA GGAAGGAGTC AGTGGTGGTG GCCATCAGAG GGACCATGTC TCTGCAGGAC 
ATCCTCACGG ACCTGTCTGC GGAGAGCGAG CCCCTCGACA TTGAGTGTGA GGCGCAGGAC 
TGCTGGGCAC ACAAGGGCAT CTCTCAGGCC GCCAGATACG TCTACCGCCG ACTCGTCAAC 
GACGGGATTC TGAGCCAAGC GTTCAGCATT GCACCTGAGT ACCGGCTGGT CGTCGTGGGG 
CACAGCCTGG GCGCGGGCGC CGCGGCGCTG CTGGCCCTCA TGCTCAAGAG CCCGTACCCG 
CAGGTCAGGT GCTACGCCTT CTCCCCGCCC AGGGGGCTGC TCAGCAAATC CCTTTATGAA 
TACTCCAAGA CCTTCATCGT GTCCCTTGTC CTGGGCAAGG ACGTGATTCC CAGGTTGAGT 
GTAACCAACC TGGAAGATCT GAAGCGGAGG ATCCTGCGAG TGATCGCCCA CTGCAACAAG 
CCCAAGTACA AGATCCTGCT GCGCGGGTGC TGGTATGAGC TGTTCGGAGG GAGCCCCGAG 
GACCTTCCCA CCGAGCTGGA TGGAGGCGAC CTGACGCAGC CCCTCCTCGG GGAGCACAGC 
CTGCTGGTGC ACGGGTCCCC GGCCTACAGC TTCTCCAGCG AGTCCCCGCT CGAGTCGCCC 
ACCAAGTACC CGCCACTCTA CCCTCCCGGC AGGATCATCC ACCTGGAGGA AGAAGGCACC 
TCAGGGAGCT GCTCCTGCTC CCCTGCCGCT CGGTACACCG TGAGGTGGGC CCACGAGTCA 
GAATTCAGCA GAATACTCAT CGGCCCCAAG ATGCTCACAG ACCACATGCC AGACATCCTG 
ATGAGGGTCC TGGACCACGT GGTGTCCGAC AGGGCTGCCT GCGTTTCCTG CCCGGCCCAG 
GGAGGCTCCA GCGTGGACCA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315972.1
CDS	16..1962
Translation	MALTVLQGLPWWVGILMLYLMHRGKLDCPGGVLLSSYLIVLLVLLAIIIGTVSAIVCVSMKGTICNPGPRKSMSKLLYVRLALFLPEMVWASLGAAWIADGIQCDRTVGNGIIATVVVSWLIIASTVLTILIVFDPLGGKAAPGAPLGPQPLDSRESSQLLSGLRTAATGVWETRVRLLCCCIGRDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDHARSPPEPTEVVTHSPGPFQEADLDAELENCHHYMQFAAAAYGWPLYVYRNPFTGLCRVGGDCCRCRTTDYDLVGGDQLNCHFSSILQTTGLQHRDFIHISFHDKVYELPFLVALDHRKESVVVAIRGTMSLQDILTDLSAESEPLDIECEAQDCWAHKGISQAARYVYRRLVNDGILSQAFSIAPEYRLVVVGHSLGAGAAALLALMLKSPYPQVRCYAFSPPRGLLSKSLYEYSKTFIVSLVLGKDVIPRLSVTNLEDLKRRILRVIAHCNKPKYKILLRGCWYELFGGSPEDLPTELDGGDLTQPLLGEHSLLVHGSPAYSFSSESPLESPTKYPPLYPPGRIIHLEEEGTSGSCSCSPAARYTVRWAHESEFSRILIGPKMLTDHMPDILMRVLDHVVSDRAACVSCPAQGGSSVDQA

Target ORF information:

RefSeq Version	XM_015460486.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase beta (DAGLB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460486.2

ORF Insert Sequence:

ATGGCTTTAA CTGTGTTACA GGGACTTCCC TGGTGGGTTG GCATCCTGAT GCTGTACCTC 
ATGCACAGAG GGAAGCTGGA CTGCCCGGGG GGCGTCCTGC TCAGCAGCTA CCTGATCGTG 
CTCCTCGTCC TCCTGGCGAT TATCATAGGC ACAGTGTCAG CCATCGTGTG TGTCAGCATG 
AAAGGGACCA TCTGTAATCC AGGACCGCGG AAATCCATGT CCAAGCTGCT GTACGTACGC 
CTGGCGCTCT TCCTGCCAGA GATGGTCTGG GCATCCCTGG GGGCCGCCTG GATCGCAGAT 
GGCATTCAGT GTGACCGGAC AGTGGGCAAC GGCATCATCG CGACCGTCGT GGTCAGCTGG 
CTCATCATCG CCTCCACCGT GCTCACCATC CTCATCGTCT TCGACCCGCT GGGGGGCAAG 
GCGGCCCCGG GCGCCCCCCT GGGCCCCCAG CCCCTGGACA GCCGAGAGTC CAGCCAGCTG 
CTGAGTGGCC TCAGGACGGC GGCGACCGGC GTGTGGGAGA CGCGAGTCAG GCTGCTGTGC 
TGCTGCATCG GCCGGGACGA CCACACTCGC GTCGCCTTTT CGAGTACGGC AGAACTTTTC 
TCAACCTACT TTTCAGACAC GGACCTGGTG CCCAGCGACA TTGCGGCCGG CCTCGCCCTT 
CTCCATCAGC AGCAGGACCA TGCACGGAGC CCCCCGGAGC CCACCGAGGT AGTCACCCAC 
TCCCCCGGGC CCTTCCAGGA GGCCGATCTG GACGCAGAGC TGGAGAACTG CCACCACTAC 
ATGCAGTTCG CAGCCGCCGC CTACGGGTGG CCCCTCTACG TCTACAGGAA CCCCTTCACG 
GGGCTCTGCC GGGTCGGCGG AGACTGCTGC AGATGCAGGA CCACCGACTA CGATTTGGTT 
GGAGGCGATC AGCTCAACTG CCACTTCAGC TCCATCCTGC AGACGACGGG GCTGCAGCAC 
CGAGACTTCA TCCACATCAG CTTCCACGAC AAGGTGTACG AGCTCCCCTT CTTGGTGGCC 
CTGGACCATA GGAAGGAGTC AGTGGTGGTG GCCATCAGAG GGACCATGTC TCTGCAGGAC 
ATCCTCACGG ACCTGTCTGC GGAGAGCGAG CCCCTCGACA TTGAGTGTGA GGCGCAGGAC 
TGCTGGGCAC ACAAGGGCAT CTCTCAGGCC GCCAGATACG TCTACCGCCG ACTCGTCAAC 
GACGGGATTC TGAGCCAAGC GTTCAGCATT GCACCTGAGT ACCGGCTGGT CGTCGTGGGG 
CACAGCCTGG GCGCGGGCGC CGCGGCGCTG CTGGCCCTCA TGCTCAAGAG CCCGTACCCG 
CAGGTCAGGT GCTACGCCTT CTCCCCGCCC AGGGGGCTGC TCAGCAAATC CCTTTATGAA 
TACTCCAAGA CCTTCATCGT GTCCCTTGTC CTGGGCAAGG ACGTGATTCC CAGGTTGAGT 
GTAACCAACC TGGAAGATCT GAAGCGGAGG ATCCTGCGAG TGATCGCCCA CTGCAACAAG 
CCCAAGTACA AGATCCTGCT GCGCGGGTGC TGGTATGAGC TGTTCGGAGG GAGCCCCGAG 
GACCTTCCCA CCGAGCTGGA TGGAGGCGAC CTGACGCAGC CCCTCCTCGG GGAGCACAGC 
CTGCTGGTGC ACGGGTCCCC GGCCTACAGC TTCTCCAGCG AGTCCCCGCT CGAGTCGCCC 
ACCAAGTACC CGCCACTCTA CCCTCCCGGC AGGATCATCC ACCTGGAGGA AGAAGGCACC 
TCAGGGAGCT GCTCCTGCTC CCCTGCCGCT CGGTACACCG TGAGGTGGGC CCACGAGTCA 
GAATTCAGCA GAATACTCAT CGGCCCCAAG ATGCTCACAG ACCACATGCC AGACATCCTG 
ATGAGGGTCC TGGACCACGT GGTGTCCGAC AGGGCTGCCT GCGTTTCCTG CCCGGCCCAG 
GGAGGCTCCA GCGTGGACCA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAGLB ( XM_015460486.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315972.1 Bos taurus diacylglycerol lipase, beta (DAGLB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa88577
Clone ID Related Accession (Same CDS sequence)	XM_015460486.2 , XM_015460486.1
Accession Version	XM_015460486.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sn1-specific diacylglycerol lipase beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104577.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAA CTGTGTTACA GGGACTTCCC TGGTGGGTTG GCATCCTGAT GCTGTACCTC 
ATGCACAGAG GGAAGCTGGA CTGCCCGGGG GGCGTCCTGC TCAGCAGCTA CCTGATCGTG 
CTCCTCGTCC TCCTGGCGAT TATCATAGGC ACAGTGTCAG CCATCGTGTG TGTCAGCATG 
AAAGGGACCA TCTGTAATCC AGGACCGCGG AAATCCATGT CCAAGCTGCT GTACGTACGC 
CTGGCGCTCT TCCTGCCAGA GATGGTCTGG GCATCCCTGG GGGCCGCCTG GATCGCAGAT 
GGCATTCAGT GTGACCGGAC AGTGGGCAAC GGCATCATCG CGACCGTCGT GGTCAGCTGG 
CTCATCATCG CCTCCACCGT GCTCACCATC CTCATCGTCT TCGACCCGCT GGGGGGCAAG 
GCGGCCCCGG GCGCCCCCCT GGGCCCCCAG CCCCTGGACA GCCGAGAGTC CAGCCAGCTG 
CTGAGTGGCC TCAGGACGGC GGCGACCGGC GTGTGGGAGA CGCGAGTCAG GCTGCTGTGC 
TGCTGCATCG GCCGGGACGA CCACACTCGC GTCGCCTTTT CGAGTACGGC AGAACTTTTC 
TCAACCTACT TTTCAGACAC GGACCTGGTG CCCAGCGACA TTGCGGCCGG CCTCGCCCTT 
CTCCATCAGC AGCAGGACCA TGCACGGAGC CCCCCGGAGC CCACCGAGGT AGTCACCCAC 
TCCCCCGGGC CCTTCCAGGA GGCCGATCTG GACGCAGAGC TGGAGAACTG CCACCACTAC 
ATGCAGTTCG CAGCCGCCGC CTACGGGTGG CCCCTCTACG TCTACAGGAA CCCCTTCACG 
GGGCTCTGCC GGGTCGGCGG AGACTGCTGC AGATGCAGGA CCACCGACTA CGATTTGGTT 
GGAGGCGATC AGCTCAACTG CCACTTCAGC TCCATCCTGC AGACGACGGG GCTGCAGCAC 
CGAGACTTCA TCCACATCAG CTTCCACGAC AAGGTGTACG AGCTCCCCTT CTTGGTGGCC 
CTGGACCATA GGAAGGAGTC AGTGGTGGTG GCCATCAGAG GGACCATGTC TCTGCAGGAC 
ATCCTCACGG ACCTGTCTGC GGAGAGCGAG CCCCTCGACA TTGAGTGTGA GGCGCAGGAC 
TGCTGGGCAC ACAAGGGCAT CTCTCAGGCC GCCAGATACG TCTACCGCCG ACTCGTCAAC 
GACGGGATTC TGAGCCAAGC GTTCAGCATT GCACCTGAGT ACCGGCTGGT CGTCGTGGGG 
CACAGCCTGG GCGCGGGCGC CGCGGCGCTG CTGGCCCTCA TGCTCAAGAG CCCGTACCCG 
CAGGTCAGGT GCTACGCCTT CTCCCCGCCC AGGGGGCTGC TCAGCAAATC CCTTTATGAA 
TACTCCAAGA CCTTCATCGT GTCCCTTGTC CTGGGCAAGG ACGTGATTCC CAGGTTGAGT 
GTAACCAACC TGGAAGATCT GAAGCGGAGG ATCCTGCGAG TGATCGCCCA CTGCAACAAG 
CCCAAGTACA AGATCCTGCT GCGCGGGTGC TGGTATGAGC TGTTCGGAGG GAGCCCCGAG 
GACCTTCCCA CCGAGCTGGA TGGAGGCGAC CTGACGCAGC CCCTCCTCGG GGAGCACAGC 
CTGCTGGTGC ACGGGTCCCC GGCCTACAGC TTCTCCAGCG AGTCCCCGCT CGAGTCGCCC 
ACCAAGTACC CGCCACTCTA CCCTCCCGGC AGGATCATCC ACCTGGAGGA AGAAGGCACC 
TCAGGGAGCT GCTCCTGCTC CCCTGCCGCT CGGTACACCG TGAGGTGGGC CCACGAGTCA 
GAATTCAGCA GAATACTCAT CGGCCCCAAG ATGCTCACAG ACCACATGCC AGACATCCTG 
ATGAGGGTCC TGGACCACGT GGTGTCCGAC AGGGCTGCCT GCGTTTCCTG CCCGGCCCAG 
GGAGGCTCCA GCGTGGACCA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315972.1
CDS	109..2055
Translation	MALTVLQGLPWWVGILMLYLMHRGKLDCPGGVLLSSYLIVLLVLLAIIIGTVSAIVCVSMKGTICNPGPRKSMSKLLYVRLALFLPEMVWASLGAAWIADGIQCDRTVGNGIIATVVVSWLIIASTVLTILIVFDPLGGKAAPGAPLGPQPLDSRESSQLLSGLRTAATGVWETRVRLLCCCIGRDDHTRVAFSSTAELFSTYFSDTDLVPSDIAAGLALLHQQQDHARSPPEPTEVVTHSPGPFQEADLDAELENCHHYMQFAAAAYGWPLYVYRNPFTGLCRVGGDCCRCRTTDYDLVGGDQLNCHFSSILQTTGLQHRDFIHISFHDKVYELPFLVALDHRKESVVVAIRGTMSLQDILTDLSAESEPLDIECEAQDCWAHKGISQAARYVYRRLVNDGILSQAFSIAPEYRLVVVGHSLGAGAAALLALMLKSPYPQVRCYAFSPPRGLLSKSLYEYSKTFIVSLVLGKDVIPRLSVTNLEDLKRRILRVIAHCNKPKYKILLRGCWYELFGGSPEDLPTELDGGDLTQPLLGEHSLLVHGSPAYSFSSESPLESPTKYPPLYPPGRIIHLEEEGTSGSCSCSPAARYTVRWAHESEFSRILIGPKMLTDHMPDILMRVLDHVVSDRAACVSCPAQGGSSVDQA

Target ORF information:

RefSeq Version	XM_015460486.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol lipase, beta (DAGLB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460486.1

ORF Insert Sequence:

ATGGCTTTAA CTGTGTTACA GGGACTTCCC TGGTGGGTTG GCATCCTGAT GCTGTACCTC 
ATGCACAGAG GGAAGCTGGA CTGCCCGGGG GGCGTCCTGC TCAGCAGCTA CCTGATCGTG 
CTCCTCGTCC TCCTGGCGAT TATCATAGGC ACAGTGTCAG CCATCGTGTG TGTCAGCATG 
AAAGGGACCA TCTGTAATCC AGGACCGCGG AAATCCATGT CCAAGCTGCT GTACGTACGC 
CTGGCGCTCT TCCTGCCAGA GATGGTCTGG GCATCCCTGG GGGCCGCCTG GATCGCAGAT 
GGCATTCAGT GTGACCGGAC AGTGGGCAAC GGCATCATCG CGACCGTCGT GGTCAGCTGG 
CTCATCATCG CCTCCACCGT GCTCACCATC CTCATCGTCT TCGACCCGCT GGGGGGCAAG 
GCGGCCCCGG GCGCCCCCCT GGGCCCCCAG CCCCTGGACA GCCGAGAGTC CAGCCAGCTG 
CTGAGTGGCC TCAGGACGGC GGCGACCGGC GTGTGGGAGA CGCGAGTCAG GCTGCTGTGC 
TGCTGCATCG GCCGGGACGA CCACACTCGC GTCGCCTTTT CGAGTACGGC AGAACTTTTC 
TCAACCTACT TTTCAGACAC GGACCTGGTG CCCAGCGACA TTGCGGCCGG CCTCGCCCTT 
CTCCATCAGC AGCAGGACCA TGCACGGAGC CCCCCGGAGC CCACCGAGGT AGTCACCCAC 
TCCCCCGGGC CCTTCCAGGA GGCCGATCTG GACGCAGAGC TGGAGAACTG CCACCACTAC 
ATGCAGTTCG CAGCCGCCGC CTACGGGTGG CCCCTCTACG TCTACAGGAA CCCCTTCACG 
GGGCTCTGCC GGGTCGGCGG AGACTGCTGC AGATGCAGGA CCACCGACTA CGATTTGGTT 
GGAGGCGATC AGCTCAACTG CCACTTCAGC TCCATCCTGC AGACGACGGG GCTGCAGCAC 
CGAGACTTCA TCCACATCAG CTTCCACGAC AAGGTGTACG AGCTCCCCTT CTTGGTGGCC 
CTGGACCATA GGAAGGAGTC AGTGGTGGTG GCCATCAGAG GGACCATGTC TCTGCAGGAC 
ATCCTCACGG ACCTGTCTGC GGAGAGCGAG CCCCTCGACA TTGAGTGTGA GGCGCAGGAC 
TGCTGGGCAC ACAAGGGCAT CTCTCAGGCC GCCAGATACG TCTACCGCCG ACTCGTCAAC 
GACGGGATTC TGAGCCAAGC GTTCAGCATT GCACCTGAGT ACCGGCTGGT CGTCGTGGGG 
CACAGCCTGG GCGCGGGCGC CGCGGCGCTG CTGGCCCTCA TGCTCAAGAG CCCGTACCCG 
CAGGTCAGGT GCTACGCCTT CTCCCCGCCC AGGGGGCTGC TCAGCAAATC CCTTTATGAA 
TACTCCAAGA CCTTCATCGT GTCCCTTGTC CTGGGCAAGG ACGTGATTCC CAGGTTGAGT 
GTAACCAACC TGGAAGATCT GAAGCGGAGG ATCCTGCGAG TGATCGCCCA CTGCAACAAG 
CCCAAGTACA AGATCCTGCT GCGCGGGTGC TGGTATGAGC TGTTCGGAGG GAGCCCCGAG 
GACCTTCCCA CCGAGCTGGA TGGAGGCGAC CTGACGCAGC CCCTCCTCGG GGAGCACAGC 
CTGCTGGTGC ACGGGTCCCC GGCCTACAGC TTCTCCAGCG AGTCCCCGCT CGAGTCGCCC 
ACCAAGTACC CGCCACTCTA CCCTCCCGGC AGGATCATCC ACCTGGAGGA AGAAGGCACC 
TCAGGGAGCT GCTCCTGCTC CCCTGCCGCT CGGTACACCG TGAGGTGGGC CCACGAGTCA 
GAATTCAGCA GAATACTCAT CGGCCCCAAG ATGCTCACAG ACCACATGCC AGACATCCTG 
ATGAGGGTCC TGGACCACGT GGTGTCCGAC AGGGCTGCCT GCGTTTCCTG CCCGGCCCAG 
GGAGGCTCCA GCGTGGACCA GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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DAGLB cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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