UBE2O cDNA ORF clone, Bos taurus(cattle)

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227028 XM_002696167.5
Latest version!
Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa227029 XM_005221305.4
Latest version!
Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa227030 XM_024981049.1
Latest version!
Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa227030 XM_024981050.1
Latest version!
Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa30452 XM_015468273.1
Latest version!
Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30452 XM_002696167.4
Latest version!
Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30453 XM_015468274.1
Latest version!
Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30453 XM_005221305.3
Latest version!
Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227028
    Clone ID Related Accession (Same CDS sequence) XM_002696167.5
    Accession Version XM_002696167.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696167.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGATTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696213.1
    CDS79..3942
    Translation

    Target ORF information:

    RefSeq Version XM_002696167.5
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696167.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGATTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227029
    Clone ID Related Accession (Same CDS sequence) XM_005221305.4
    Accession Version XM_005221305.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221305.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGATT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221362.1
    CDS74..3862
    Translation

    Target ORF information:

    RefSeq Version XM_005221305.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221305.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCAGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATTAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGATT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227030
    Clone ID Related Accession (Same CDS sequence) XM_024981049.1 , XM_024981050.1
    Accession Version XM_024981049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
    AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC
    TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC
    CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG
    GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT
    GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC
    GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG
    GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA
    GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG
    GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT
    GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC
    GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG
    AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA
    GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG
    CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG
    GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG
    GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC
    TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC
    ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA
    CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG
    GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT
    GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC
    CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC
    ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA
    GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA
    ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG
    CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT
    GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG
    GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA
    GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA
    GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG
    GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC
    GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG
    AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA
    GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA
    GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC
    GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG
    CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT
    GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC
    GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC
    TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG
    GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC
    ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC
    GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT
    GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG
    TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG
    AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC
    GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG
    CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT
    GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG
    GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG
    GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG
    GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG
    GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA
    AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC
    TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT
    GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836817.1
    CDS445..3849
    Translation

    Target ORF information:

    RefSeq Version XM_024981049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
    AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC
    TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC
    CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG
    GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT
    GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC
    GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG
    GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA
    GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG
    GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT
    GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC
    GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG
    AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA
    GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG
    CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG
    GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG
    GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC
    TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC
    ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA
    CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG
    GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT
    GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC
    CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC
    ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA
    GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA
    ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG
    CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT
    GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG
    GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA
    GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA
    GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG
    GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC
    GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG
    AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA
    GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA
    GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC
    GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG
    CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT
    GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC
    GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC
    TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG
    GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC
    ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC
    GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT
    GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG
    TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG
    AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC
    GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG
    CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT
    GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG
    GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG
    GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG
    GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG
    GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA
    AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC
    TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT
    GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227030
    Clone ID Related Accession (Same CDS sequence) XM_024981049.1 , XM_024981050.1
    Accession Version XM_024981050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3405bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
    AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC
    TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC
    CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG
    GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT
    GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC
    GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG
    GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA
    GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG
    GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT
    GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC
    GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG
    AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA
    GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG
    CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG
    GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG
    GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC
    TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC
    ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA
    CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG
    GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT
    GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC
    CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC
    ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA
    GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA
    ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG
    CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT
    GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG
    GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA
    GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA
    GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG
    GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC
    GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG
    AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA
    GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA
    GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC
    GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG
    CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT
    GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC
    GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC
    TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG
    GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC
    ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC
    GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT
    GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG
    TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG
    AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC
    GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG
    CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT
    GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG
    GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG
    GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG
    GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG
    GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA
    AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC
    TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT
    GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836818.1
    CDS230..3634
    Translation

    Target ORF information:

    RefSeq Version XM_024981050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGCTCCA CTGACAGCCA GTGCGGCACC GTGATCGACG TGAACATTGA CTGCGCCGTC 
    AAGCTCATCG GCACCAACTG CATCGTCTAC CCCGTCAACA GCAAGGACCT GCAGCACATC
    TGGCCCTTCA TGTACGGGGA CTACATCGCC TACGACTGCT GGCTGGGCAA GGTCTACGAC
    CTGAAGAACC AGATCATCCT GAAACTGTCC AACGGCGCCA GGTGCTCCAT GAACACGGAG
    GATGGCGCCA AGCTCTACGA CGTCTGCCCG CACGTCAGCG ACTCTGGTCT CTTCTTCGAT
    GATTCCTATG GCTTCTACCC GGGCCAGGTG CTCATTGGCC CTGCCAAGAT CTTCTCCAGC
    GTCCAGTGGC TGTCGGGCGT CAAGCCAGTG CTCAGCACCA AGAGCAAGTT CCGCGTGGTG
    GTCGAAGAGG TGCAGGTTGT AGAGCTGAAG GTCACCTGGA TCACCAAGAG CTTCTGTCCA
    GGGGGCACGG ACAGCGTCAG CCCCCCACCC TCTGTCATTA CCCAGGAAAA CCTAGGCAGG
    GTGAAGCGTC TCGGATGCTT TGACCATGCT CAGCGGCAGC TTGGGGAGCG CTGTCTGTAT
    GTCTTCCCAG CCAAGGTAGA GCCGGCCAAG ATTGCCTGTG AATGTCCAGA AAAACACTGC
    GCCCAGGGGG AGGGCTCTGT GGCCAAGAAG GTGAAGCGCC TCCTGAAGAA ACAGCTTGTG
    AGGATCACAT CTTGCTCCCC GGACGTCCAG TGCTCCAGGG ACCATTCCAT GGAGGACGCA
    GGCAAGAAGG GGGCGGCCAA AGCCAAGAGC GAAACGGGCT CCGCCAGCCC CGAGGAGACG
    CCCGATGGGT CCTCCAGCCC AGTGGAGATG CAGGACGAGG GCCCAGAGGA GGCCCACGAG
    GCGGGTGAGC AGCTGCCCCC CTTCCTGCTG AAGGAGGGTG GGGACGACAG GCTGCACTCG
    GCCGAGCAGG ACGCGGACGA TGAGGCCGCC GACGACACAG ACGACACCAG CTCGGTGACC
    TCCTCCGCCA GCTCCACCAC CTCCTCCCAG AGCGGCAGCG GCACGAGTCG CAAGAAGAGC
    ATCCCCTTGT CAATAAAGAA TTTAAAGAGG AAACACAAGA GGAAGAAGAA TAAAATCACA
    CGCGACTTCA AGCCGGGGGA CAGGGTGGCT GTGGAGGTGG TGACCACGAT GACCTCGGCG
    GACGTGATGT GGCAGGACGG CTCCGTGGAG TGCAACATCC GCTCCAATGA CCTCTTCCCT
    GTGCACCACC TGGACAACAA CGAGTTCTGC CCCGGAGACT TCGTGGTGGA CAAGCGAGTC
    CAGAGCTGTC CGGACCCCGC TGTCTATGGC GTGGTGCAGT CTGGGGACCA CGTGGGCCGC
    ACGTGCATGG TGAAGTGGTT CAAGCTGCGG CCGAGCGGTG ACGACGTGGA GCTGATCGGA
    GAGGAGGAAG ACGTGAGCGT CTACGACATC GCCGATCACC CTGATTTCCG GTTCCGCACA
    ACTGACATCG TCATCCGAAT CGGTAACGCT GAGGACGGAG CCCCGCACAA GGAGGACGAG
    CCATCGGTAG GCCAGGTAGC CCGCGTGGAT GTCAGCAGCA AAGTGGAGGT GGTGTGGGCT
    GACAACTCAA AGACCATCAT CCTGCCCCAG CACCTGTACA ACATCGAGTC TGAGATCGAG
    GAGTCGGACT ACGACTCAGT GGAAGGCAGC ACCAGTGGAG CGTCCTCAGA CGAGTGGGAA
    GACGACAGTG ACAGCTGGGA GACAGACAAT GGGCTGGTGG AGGATGAGCA CCCCAAGATA
    GAGGAGCCCC CCATCCCCAC CGCCGAGCAG CCAGCAGCCC CAGAGGAGGA CAAGGGTGTG
    GTGATCAGCG AGGAGGCTGC CACGGCCGCC ATCCAGGGGG CCGTGGCCAT GGCCGCCCCC
    GTGGCCGGGC TGATGGAGAA GGCGGGCAAG GACGGGCCCC CGAAGAGCTT CCGGGAGCTG
    AAGGAAGCCA TCAAGATCCT GGAGAGCCTC AAGAACATGA CGGTGGAGCA GCTGCTGACA
    GGCTCGCCCA CCTCCCCCAC GGCAGAGCCT GAGAAACCCA CGCGGGAGAA GAAGTTCTTA
    GATGACATCA AGAAGCTGCA GGAGAACCTC AAGAAGACCC TGGACAATGT GGCCATCGCC
    GAGGAAGAGA AGATGGAGGC GGTGCCGGAG ACGGAGCGCA AGGAGGACAA GCCCGAGGGG
    CAGTCGCCAG TGAAGGCCGA GTGGCCCAGT GAGACCCCGG TGCTCTGCCA GCAGTGCGGT
    GGCAAGCCTG GAGTCACATT CACCAGCGCC AAGGGCGAGG TCTTCTCGGT GCTGGAGTTC
    GCACCCTCGA ATCACTCTTT TAAGAAAATT GAGTTCCAGC CTCCAGAAGC CAAGAAGTTC
    TTCAGCACAG TGCGGAAGGA GATGGCACTG CTGGCCACCT CACTGCCTGA TGGCATCATG
    GTCAAGACAT TCGAGGACAG AATGGACCTC TTCTCGGCCC TGATCAAGGG CCCCACTCGC
    ACCCCCTACG AGGACGGCCT CTACTTGTTC GACATCCAGC TCCCCAACAT CTACCCGGCC
    GTGCCCCCCC ACTTCTGCTA CCTCTCCCAG TGCAGTGGCC GCCTGAACCC CAACCTGTAT
    GACAACGGGA AGGTGTGCGT CAGCCTCCTG GGCACCTGGA TTGGAAAGGG GACAGAGAGG
    TGGACGAGCA AATCCAGCCT TCTCCAGGTG CTTATCTCCA TCCAAGGTCT AATCCTGGTG
    AACGAACCGT ACTACAACGA GGCAGGCTTT GACAGCGACC GAGGCCTGCA AGAGGGCTAC
    GAGAACAGCC GCTGCTACAA CGAGATGGCC CTCATCCGCG TGGTACAGTC CATGACCCAG
    CTGGTCCGGC GGCCACCCGA GGTCTTTGAG CAGGAGATCC GGCAGCACTT TAGCACCGGT
    GGCTGGCGGC TGGTGAACCG CATTGAGTCC TGGCTGGAAA CCCACGCCCT GCTGGAGAGG
    GCCCAGGCGC TGCCCAACGG GCTCCCCAAG GACAGCAGCT CGCCGGAGCC GCCGGCTGTG
    GCCGAGCTCT CAGATTTCGG CCGAGAGGAA CCTGAGGACG GGGGGCTGGC CCCCGGGGAG
    GCCTCCCAGG GCTCAGACTC GGAGGGCCCG GCCTCAGCCC CCAGGGACCG CACGGAGCAG
    GCCTCGGAGG CCCCGCCCGA CGCCTCGGTG CCGCCCAGCG TCAAGCCAAA GAAGAGGAGA
    AAGAGCTACC GGAGCTTCCT GCCCGAGAAG AGCGGCTACC CTGACCTCGG CTTCCCCCTC
    TTCCCGCTCT CCAAGGGCTT CGTCAAGAGC ATCCGGGGAG TCCTGATGCA GTTCCGGGCT
    GCCCTGACGG AGGCGGGCAT GCCCGAGAGC GCGGGGGACA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30452
    Clone ID Related Accession (Same CDS sequence) XM_002696167.4 , XM_015468273.1
    Accession Version XM_002696167.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696167.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696213.1
    CDS39..3902
    Translation

    Target ORF information:

    RefSeq Version XM_002696167.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696167.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30452
    Clone ID Related Accession (Same CDS sequence) XM_002696167.4 , XM_015468273.1
    Accession Version XM_015468273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323759.1
    CDS39..3902
    Translation

    Target ORF information:

    RefSeq Version XM_015468273.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTAGAG
    CCGGCCAAGA TTGCCTGTGA ATGTCCAGAA AAACACTGCG CCCAGGGGGA GGGCTCTGTG
    GCCAAGAAGG TGAAGCGCCT CCTGAAGAAA CAGCTTGTGA GGATCACATC TTGCTCCCCG
    GACGTCCAGT GCTCCAGGGA CCATTCCATG GAGGACGCAG GCAAGAAGGG GGCGGCCAAA
    GCCAAGAGCG AAACGGGCTC CGCCAGCCCC GAGGAGACGC CCGATGGGTC CTCCAGCCCA
    GTGGAGATGC AGGACGAGGG CCCAGAGGAG GCCCACGAGG CGGGTGAGCA GCTGCCCCCC
    TTCCTGCTGA AGGAGGGTGG GGACGACAGG CTGCACTCGG CCGAGCAGGA CGCGGACGAT
    GAGGCCGCCG ACGACACAGA CGACACCAGC TCGGTGACCT CCTCCGCCAG CTCCACCACC
    TCCTCCCAGA GCGGCAGCGG CACGAGTCGC AAGAAGAGCA TCCCCTTGTC AATAAAGAAT
    TTAAAGAGGA AACACAAGAG GAAGAAGAAT AAAATCACAC GCGACTTCAA GCCGGGGGAC
    AGGGTGGCTG TGGAGGTGGT GACCACGATG ACCTCGGCGG ACGTGATGTG GCAGGACGGC
    TCCGTGGAGT GCAACATCCG CTCCAATGAC CTCTTCCCTG TGCACCACCT GGACAACAAC
    GAGTTCTGCC CCGGAGACTT CGTGGTGGAC AAGCGAGTCC AGAGCTGTCC GGACCCCGCT
    GTCTATGGCG TGGTGCAGTC TGGGGACCAC GTGGGCCGCA CGTGCATGGT GAAGTGGTTC
    AAGCTGCGGC CGAGCGGTGA CGACGTGGAG CTGATCGGAG AGGAGGAAGA CGTGAGCGTC
    TACGACATCG CCGATCACCC TGATTTCCGG TTCCGCACAA CTGACATCGT CATCCGAATC
    GGTAACGCTG AGGACGGAGC CCCGCACAAG GAGGACGAGC CATCGGTAGG CCAGGTAGCC
    CGCGTGGATG TCAGCAGCAA AGTGGAGGTG GTGTGGGCTG ACAACTCAAA GACCATCATC
    CTGCCCCAGC ACCTGTACAA CATCGAGTCT GAGATCGAGG AGTCGGACTA CGACTCAGTG
    GAAGGCAGCA CCAGTGGAGC GTCCTCAGAC GAGTGGGAAG ACGACAGTGA CAGCTGGGAG
    ACAGACAATG GGCTGGTGGA GGATGAGCAC CCCAAGATAG AGGAGCCCCC CATCCCCACC
    GCCGAGCAGC CAGCAGCCCC AGAGGAGGAC AAGGGTGTGG TGATCAGCGA GGAGGCTGCC
    ACGGCCGCCA TCCAGGGGGC CGTGGCCATG GCCGCCCCCG TGGCCGGGCT GATGGAGAAG
    GCGGGCAAGG ACGGGCCCCC GAAGAGCTTC CGGGAGCTGA AGGAAGCCAT CAAGATCCTG
    GAGAGCCTCA AGAACATGAC GGTGGAGCAG CTGCTGACAG GCTCGCCCAC CTCCCCCACG
    GCAGAGCCTG AGAAACCCAC GCGGGAGAAG AAGTTCTTAG ATGACATCAA GAAGCTGCAG
    GAGAACCTCA AGAAGACCCT GGACAATGTG GCCATCGCCG AGGAAGAGAA GATGGAGGCG
    GTGCCGGAGA CGGAGCGCAA GGAGGACAAG CCCGAGGGGC AGTCGCCAGT GAAGGCCGAG
    TGGCCCAGTG AGACCCCGGT GCTCTGCCAG CAGTGCGGTG GCAAGCCTGG AGTCACATTC
    ACCAGCGCCA AGGGCGAGGT CTTCTCGGTG CTGGAGTTCG CACCCTCGAA TCACTCTTTT
    AAGAAAATTG AGTTCCAGCC TCCAGAAGCC AAGAAGTTCT TCAGCACAGT GCGGAAGGAG
    ATGGCACTGC TGGCCACCTC ACTGCCTGAT GGCATCATGG TCAAGACATT CGAGGACAGA
    ATGGACCTCT TCTCGGCCCT GATCAAGGGC CCCACTCGCA CCCCCTACGA GGACGGCCTC
    TACTTGTTCG ACATCCAGCT CCCCAACATC TACCCGGCCG TGCCCCCCCA CTTCTGCTAC
    CTCTCCCAGT GCAGTGGCCG CCTGAACCCC AACCTGTATG ACAACGGGAA GGTGTGCGTC
    AGCCTCCTGG GCACCTGGAT TGGAAAGGGG ACAGAGAGGT GGACGAGCAA ATCCAGCCTT
    CTCCAGGTGC TTATCTCCAT CCAAGGTCTA ATCCTGGTGA ACGAACCGTA CTACAACGAG
    GCAGGCTTTG ACAGCGACCG AGGCCTGCAA GAGGGCTACG AGAACAGCCG CTGCTACAAC
    GAGATGGCCC TCATCCGCGT GGTACAGTCC ATGACCCAGC TGGTCCGGCG GCCACCCGAG
    GTCTTTGAGC AGGAGATCCG GCAGCACTTT AGCACCGGTG GCTGGCGGCT GGTGAACCGC
    ATTGAGTCCT GGCTGGAAAC CCACGCCCTG CTGGAGAGGG CCCAGGCGCT GCCCAACGGG
    CTCCCCAAGG ACAGCAGCTC GCCGGAGCCG CCGGCTGTGG CCGAGCTCTC AGACTTCGGC
    CGAGAGGAAC CTGAGGACGG GGGGCTGGCC CCCGGGGAGG CCTCCCAGGG CTCAGACTCG
    GAGGGCCCGG CCTCAGCCCC CAGGGACCGC ACGGAGCAGG CCTCGGAGGC CCCGCCCGAC
    GCCTCGGTGC CGCCCAGCGT CAAGCCAAAG AAGAGGAGAA AGAGCTACCG GAGCTTCCTG
    CCCGAGAAGA GCGGCTACCC TGACCTCGGC TTCCCCCTCT TCCCGCTCTC CAAGGGCTTC
    GTCAAGAGCA TCCGGGGAGT CCTGATGCAG TTCCGGGCTG CCCTGACGGA GGCGGGCATG
    CCCGAGAGCG CGGGGGACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30453
    Clone ID Related Accession (Same CDS sequence) XM_015468274.1 , XM_005221305.3
    Accession Version XM_015468274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323760.1
    CDS39..3827
    Translation

    Target ORF information:

    RefSeq Version XM_015468274.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30453
    Clone ID Related Accession (Same CDS sequence) XM_015468274.1 , XM_005221305.3
    Accession Version XM_005221305.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221362.1
    CDS39..3827
    Translation

    Target ORF information:

    RefSeq Version XM_005221305.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ubiquitin conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221305.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGGATC CCGCAGCGTC AGCGCCCGCA GCCCCGGCTC CCGCCCAGAT CCCGGACGTG 
    CCCCCGAGCC CGGACGCAGC CCCCGCCTCG GCCCCGGCGC CGGCGTCCGC GGCGGACTCG
    GGCTCCGGGC CATCCTCGGA CTCTGGCCCC GAAGCCGGCT CGCAGCGGTT GCTCTTCTCC
    CACGACCTGG TGTCGGGCCG TTACCGCGGC TCGGTGCACT TCGGGCTGGT GCGCCTCATC
    CACGGCGAGG ACTCGGACTC GGAGGGCGAA GAGGAGGGCC GTGGGAGCTC GGGCTGCTCG
    GAGGCTGGGG GCGCGGGCCA CGAGGAGGGC CGGGCCAGCC CGCTGCGCCG CGGCTACGTG
    CGCGTCCAGT GGTACCCGGA GGGCGTCAAG CAGCACGTGA AGGAGACCAA GCTGAAGCTC
    GAGGACCGCT CCGTGGTGCC CCGCGATGTG GTCCGGCACA TGCGCTCCAC TGACAGCCAG
    TGCGGCACCG TGATCGACGT GAACATTGAC TGCGCCGTCA AGCTCATCGG CACCAACTGC
    ATCGTCTACC CCGTCAACAG CAAGGACCTG CAGCACATCT GGCCCTTCAT GTACGGGGAC
    TACATCGCCT ACGACTGCTG GCTGGGCAAG GTCTACGACC TGAAGAACCA GATCATCCTG
    AAACTGTCCA ACGGCGCCAG GTGCTCCATG AACACGGAGG ATGGCGCCAA GCTCTACGAC
    GTCTGCCCGC ACGTCAGCGA CTCTGGTCTC TTCTTCGATG ATTCCTATGG CTTCTACCCG
    GGCCAGGTGC TCATTGGCCC TGCCAAGATC TTCTCCAGCG TCCAGTGGCT GTCGGGCGTC
    AAGCCGGTGC TCAGCACCAA GAGCAAGTTC CGCGTGGTGG TCGAAGAGGT GCAGGTTGTA
    GAGCTGAAGG TCACCTGGAT CACCAAGAGC TTCTGTCCAG GGGGCACGGA CAGCGTCAGC
    CCCCCACCCT CTGTCATCAC CCAGGAAAAC CTAGGCAGGG TGAAGCGTCT CGGATGCTTT
    GACCATGCTC AGCGGCAGCT TGGGGAGCGC TGTCTGTATG TCTTCCCAGC CAAGGTGAAG
    CGCCTCCTGA AGAAACAGCT TGTGAGGATC ACATCTTGCT CCCCGGACGT CCAGTGCTCC
    AGGGACCATT CCATGGAGGA CGCAGGCAAG AAGGGGGCGG CCAAAGCCAA GAGCGAAACG
    GGCTCCGCCA GCCCCGAGGA GACGCCCGAT GGGTCCTCCA GCCCAGTGGA GATGCAGGAC
    GAGGGCCCAG AGGAGGCCCA CGAGGCGGGT GAGCAGCTGC CCCCCTTCCT GCTGAAGGAG
    GGTGGGGACG ACAGGCTGCA CTCGGCCGAG CAGGACGCGG ACGATGAGGC CGCCGACGAC
    ACAGACGACA CCAGCTCGGT GACCTCCTCC GCCAGCTCCA CCACCTCCTC CCAGAGCGGC
    AGCGGCACGA GTCGCAAGAA GAGCATCCCC TTGTCAATAA AGAATTTAAA GAGGAAACAC
    AAGAGGAAGA AGAATAAAAT CACACGCGAC TTCAAGCCGG GGGACAGGGT GGCTGTGGAG
    GTGGTGACCA CGATGACCTC GGCGGACGTG ATGTGGCAGG ACGGCTCCGT GGAGTGCAAC
    ATCCGCTCCA ATGACCTCTT CCCTGTGCAC CACCTGGACA ACAACGAGTT CTGCCCCGGA
    GACTTCGTGG TGGACAAGCG AGTCCAGAGC TGTCCGGACC CCGCTGTCTA TGGCGTGGTG
    CAGTCTGGGG ACCACGTGGG CCGCACGTGC ATGGTGAAGT GGTTCAAGCT GCGGCCGAGC
    GGTGACGACG TGGAGCTGAT CGGAGAGGAG GAAGACGTGA GCGTCTACGA CATCGCCGAT
    CACCCTGATT TCCGGTTCCG CACAACTGAC ATCGTCATCC GAATCGGTAA CGCTGAGGAC
    GGAGCCCCGC ACAAGGAGGA CGAGCCATCG GTAGGCCAGG TAGCCCGCGT GGATGTCAGC
    AGCAAAGTGG AGGTGGTGTG GGCTGACAAC TCAAAGACCA TCATCCTGCC CCAGCACCTG
    TACAACATCG AGTCTGAGAT CGAGGAGTCG GACTACGACT CAGTGGAAGG CAGCACCAGT
    GGAGCGTCCT CAGACGAGTG GGAAGACGAC AGTGACAGCT GGGAGACAGA CAATGGGCTG
    GTGGAGGATG AGCACCCCAA GATAGAGGAG CCCCCCATCC CCACCGCCGA GCAGCCAGCA
    GCCCCAGAGG AGGACAAGGG TGTGGTGATC AGCGAGGAGG CTGCCACGGC CGCCATCCAG
    GGGGCCGTGG CCATGGCCGC CCCCGTGGCC GGGCTGATGG AGAAGGCGGG CAAGGACGGG
    CCCCCGAAGA GCTTCCGGGA GCTGAAGGAA GCCATCAAGA TCCTGGAGAG CCTCAAGAAC
    ATGACGGTGG AGCAGCTGCT GACAGGCTCG CCCACCTCCC CCACGGCAGA GCCTGAGAAA
    CCCACGCGGG AGAAGAAGTT CTTAGATGAC ATCAAGAAGC TGCAGGAGAA CCTCAAGAAG
    ACCCTGGACA ATGTGGCCAT CGCCGAGGAA GAGAAGATGG AGGCGGTGCC GGAGACGGAG
    CGCAAGGAGG ACAAGCCCGA GGGGCAGTCG CCAGTGAAGG CCGAGTGGCC CAGTGAGACC
    CCGGTGCTCT GCCAGCAGTG CGGTGGCAAG CCTGGAGTCA CATTCACCAG CGCCAAGGGC
    GAGGTCTTCT CGGTGCTGGA GTTCGCACCC TCGAATCACT CTTTTAAGAA AATTGAGTTC
    CAGCCTCCAG AAGCCAAGAA GTTCTTCAGC ACAGTGCGGA AGGAGATGGC ACTGCTGGCC
    ACCTCACTGC CTGATGGCAT CATGGTCAAG ACATTCGAGG ACAGAATGGA CCTCTTCTCG
    GCCCTGATCA AGGGCCCCAC TCGCACCCCC TACGAGGACG GCCTCTACTT GTTCGACATC
    CAGCTCCCCA ACATCTACCC GGCCGTGCCC CCCCACTTCT GCTACCTCTC CCAGTGCAGT
    GGCCGCCTGA ACCCCAACCT GTATGACAAC GGGAAGGTGT GCGTCAGCCT CCTGGGCACC
    TGGATTGGAA AGGGGACAGA GAGGTGGACG AGCAAATCCA GCCTTCTCCA GGTGCTTATC
    TCCATCCAAG GTCTAATCCT GGTGAACGAA CCGTACTACA ACGAGGCAGG CTTTGACAGC
    GACCGAGGCC TGCAAGAGGG CTACGAGAAC AGCCGCTGCT ACAACGAGAT GGCCCTCATC
    CGCGTGGTAC AGTCCATGAC CCAGCTGGTC CGGCGGCCAC CCGAGGTCTT TGAGCAGGAG
    ATCCGGCAGC ACTTTAGCAC CGGTGGCTGG CGGCTGGTGA ACCGCATTGA GTCCTGGCTG
    GAAACCCACG CCCTGCTGGA GAGGGCCCAG GCGCTGCCCA ACGGGCTCCC CAAGGACAGC
    AGCTCGCCGG AGCCGCCGGC TGTGGCCGAG CTCTCAGACT TCGGCCGAGA GGAACCTGAG
    GACGGGGGGC TGGCCCCCGG GGAGGCCTCC CAGGGCTCAG ACTCGGAGGG CCCGGCCTCA
    GCCCCCAGGG ACCGCACGGA GCAGGCCTCG GAGGCCCCGC CCGACGCCTC GGTGCCGCCC
    AGCGTCAAGC CAAAGAAGAG GAGAAAGAGC TACCGGAGCT TCCTGCCCGA GAAGAGCGGC
    TACCCTGACC TCGGCTTCCC CCTCTTCCCG CTCTCCAAGG GCTTCGTCAA GAGCATCCGG
    GGAGTCCTGA TGCAGTTCCG GGCTGCCCTG ACGGAGGCGG GCATGCCCGA GAGCGCGGGG
    GACAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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