UBE2O cDNA ORF clone, Gallus gallus(chicken)

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa16521 XM_415619.6
Latest version!
Gallus gallus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa43899 XM_015295299.2
Latest version!
Gallus gallus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OGa43899 XM_015295299.1 Gallus gallus ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa16521 XM_415619.5 Gallus gallus ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa16521
    Clone ID Related Accession (Same CDS sequence) XM_415619.5 , XM_415619.6
    Accession Version XM_415619.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_415619.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATGTGC TTTCTGTGAA GGACACGGAT CTGCTGAAGC TTGAAGATCG CTCTGTGGTC 
    CCTCGAGACG TGGTCAGACA TATGAGCTCC AGTGACAGCC AGTGTGGGAC TGTAATTGAT
    GTCAACATAG AGTGTGCTGT GAAGCTGGTA GGAACCAACT GCATCCTGTA CCCTGTCAAC
    AGCAAAGACC TGCAACATAT CTGGCCCTTC ATGTATGGTG ACTACATAGC CTACGACTGC
    TGGCTGGGCA AGGTGTACGA TCTGAAGAAC CAAGTCATCC TTAAGCTTTC CAACGGAGCC
    AGGTGTTCAA TGAGCACAGA AGATGGAGCC AAGCTGTATG ACGTCTGTCC TCACGTCAGT
    GACTCGGGTC TCTTCTTTGA CGATTCATAT GGCTTTTATC CTGGGCAAGT CCTCATCGGG
    CCTTCTAAAG TCTTCTCCAG TGTGCAATGG CTCTCGGGTG TGAAGCCTGT TCTGAGCACA
    AAAAGCAAGT TCCGAGTGGT GGTGGAAGAG GTACAGGTGG TAGAACTAAA GGTTACTTGG
    ATCACTAAAA GCTTCTGTCC TGGAGGTACT GACAGTGTGA GTCCTCCACC GTCGATCATC
    AGCCAGGAGA ACCTGTCGAG GGTAAAGCGG CTGGGGTGCT TTGACCATGC CCAGAGGCAG
    CTTGGAGAAA GGTGCCTCTA TGTGTTCCCA GACAAAGTGG AGCCTGCAAA AATCACCTGT
    GAATGCCCAG AGAGGAACTG TGTCCTGGGT GAAGGATCAG TTGCCAAAAA GGTGAGGAGA
    CTGCTGAAGA AGCAGATAGT GAAGATCATG TCGTGCTCCC CAGAGTCTCA GGGTACCACT
    GAAGCCCCGG ACAAAGAGCC TGCTCCAGCT GCTGACCAAA AGGCAGAAGA CCTTAAGCCT
    GGCTCAAAGT GTGAACTGGA GGACTCTTCC AACGGTGCAC CGAGCGCGGG GGAGAGGAAG
    GAGGAGCAGC CTGAAGAAAC AGGGAAGGAC AAAGGGGGAG CAGCAGTGAG ACAGCAGCAA
    CCTCCCTTTC TGCTGAAAGA TGAGGGATCG GCTGATTACA GGCTGCAGTC CATGGAGCAG
    GATGCTGATG ATGAGGCAGC AGATGACACG GATGACACGA GCTCTGTCAC CTCCTCCGCC
    AGCTCCACCG CCTCATCCCA GAGCGGCAGC GGCACCGGCC GCAAGAAGAG CATCCCTCTT
    TCCATCAAAA ACTTGAAGCG GAAGCACAAG AAGAAGAAGA CCAAGATCTC GAGAGAATTT
    AAGCCAGGAG ACAGGGTTGC AGTGGAGGTG GTGACCACGA TGACTTCGGC TGACGTGATG
    TGGCAGGATG GCACCGTGGA GACCAACATT CGTTCCAACG AGCTGATTCC AGTCCATCAT
    CTGGACAACA ATGAATTCTG CCCCGGGGAT TTCGTGGTGG ACAAAAGAGC TCAGAGCTCC
    CAGGACCCCG GTGTGTATGG AGTCGTGCAG TCTGGTGACC ATATCGGCCG TACCTGTGTG
    GTGAAGTGGT TCAAGCTAAA ATCCAGTGGA GATGATGTGG AGCTGATAGG GGAGGAAGAG
    GATGTGAGTG TGTACGACAT TGCTGACCAC CCAGACTTTC GCTTCCGCAC CACTGACATT
    GTGATTCGCA TCGGCAATTC GGATGGAGCC ACAGCTAACG AAGACGAGCC TTCGGTCGGA
    CAGGTGGCTC GCGTGGATGT CAGCAGTAAA GTGGAGGTGG TGTGGGCTGA TAACTCCAAG
    ACGATCATTC TGCCTCAGCA CTTATACAAC ATTGAGTCGG AGATCGAGGA GTCGGACTAT
    GACTCTGTCG ACGGCAGCAC CAGTGGGGCA TCCTCTGAGT GGGAGGATGA AAGCGACAGC
    TGGGAGACAG ACAACGGCCT CATGGAAGAC GACCACCCGA GGATTGAGGA GTTGGAGCCC
    GAGGAGCCGG CAGCAGAGGA GGTGAAAAAA GTGGAGGAAC AAGTCCAGGG AGCTGTGGCT
    ATGGCAGTAG CAGATCTTGC TGCAGAGAAA GCTGGCAAGG ACGGAGCCCC GAAGAGCTTC
    AGGGAACTAA AGGAAGCCAT CAAGATCTTG GAAAGCCTGA AGAATATGAC TGTGGAGCAG
    CTGCTGACTG GCTCTCCCAC CTCCCCAACT GTGGAGCTGG AAAAACCCAC TCGGGAGAAG
    AAATTCTTGG ACGATATTAA AAAGCTGCAG GAAAATCTCA AGAAAACCTT GGATAACGTG
    GCGATTGCAG AAGAGGAAAA GATGGAAGCC ATGGTGGAGA CAGAGAAGAA AGAGGAAAAA
    GCAGAGGCAC AGACACCGGT GAGGTCGGAG TGGCCCAGCG AGACACCAGT GCTGTGCCAG
    CAGAGTGGGG GCAAACCTGG AGTCACCTTC ACCAGTGCCA AGGGAGAGGT GTTCTCTGTG
    CTGGAGTGTG CTCCAGATAG CCATGCCTTC AAGAAAATGG AGTTCCAGCC CCCAGAAGCA
    AAGAAGTTCT TTAGCACTGT GCGGAAGGAG ATGGCCCTGC TGGCCACCTC CCTGCCAGAT
    GGCATCATGG TTAAAACCTT TGAGGATCGA ATGGACCTCT TCTCAGCACT TATAAAAGGG
    CCCACACGCA CACCCTATGA GGACGGGCTC TTTCTCTTTG ACATCCAGCT CCCCAACATC
    TACCCTGCTG TGCCGCCTCT CTTTCGCTAC CTCTCCCAGT GCAGCGGGAG GCTGAATCCC
    AACCTGTATG ACAATGGCAA AGTGTGTGTC AGCCTCCTGG GGACGTGGAT AGGCAAGGGA
    ACAGAAAGGT GGACCAGTAA ATCCAGCCTC CTCCAAGTAC TCATCTCCAT CCAAGGTCTC
    ATCCTAGTGA ACGAGCCCTA TTACAACGAG GCGGGCTTCG ACAGCGACCG GGGCCTGCAG
    GAGGGCTACG AGAACAGCCG CTGCTACAAC GAGATGACGC TGATCCGGGT GGTGCAGTCC
    ATGATGCAGC TGCTGCGCAG ACCCGTGGAG GTCTTCGAGC ACGAAATCCG CGAGCATTTC
    CGCTGCAACG GCTGGCGCCT GGTGAGCCGC ATCGAGTCCT GGCTGGAGAC CAACGAGCTG
    GTGGAGAGGA GCCACGAGCA GAGCAACGGG GCAGATCCTG CCTCCCTCCT GTCTGCCGGC
    AGCGCCCTGG CCGACAGCCC CGGAGACACC GTGGGGTCGC CGGGGGAGTT GAGCGGCTCC
    GCCGAGCAGG GAGCGGCGGC CGAACTCTCC GACTCGGCGC TGGACGGGAA GGAGGAGTTG
    GACGAGGCGG AGTTCGCGAC CTCGGCGCCC AACGCCGGCG ACGTCCGCCA GCACTCGGAC
    TCGGAGGGCG CGGGCCAAGG CAGGGGGGTG GGCCTGGCCA GAGACCGAAC GGACGAGGGG
    AGGGCTGCCC AGGACAGTGC CTCGCAGCCG AGCGTGAAAC CAAAGAAAAG GAGAAAGAGC
    TACAGGAGTT TCCTTCCGGA GAAGAGCGGT TACCCCGATA TCGGGTTCCC CCTCTTCCCT
    TTGTCCAAGG GGTTCATTAA AAGCATCCGC GGTGTCTTGC AGCAGTACCG GGCTGCCTTA
    GCAGGGGCCA ATATCCCAGA GTGGACAGAG GACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_415619.4
    CDS117..3695
    Translation

    Target ORF information:

    RefSeq Version XM_415619.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_415619.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATGTGC TTTCTGTGAA GGACACGGAT CTGCTGAAGC TTGAAGATCG CTCTGTGGTC 
    CCTCGAGACG TGGTCAGACA TATGAGCTCC AGTGACAGCC AGTGTGGGAC TGTAATTGAT
    GTCAACATAG AGTGTGCTGT GAAGCTGGTA GGAACCAACT GCATCCTGTA CCCTGTCAAC
    AGCAAAGACC TGCAACATAT CTGGCCCTTC ATGTATGGTG ACTACATAGC CTACGACTGC
    TGGCTGGGCA AGGTGTACGA TCTGAAGAAC CAAGTCATCC TTAAGCTTTC CAACGGAGCC
    AGGTGTTCAA TGAGCACAGA AGATGGAGCC AAGCTGTATG ACGTCTGTCC TCACGTCAGT
    GACTCGGGTC TCTTCTTTGA CGATTCATAT GGCTTTTATC CTGGGCAAGT CCTCATCGGG
    CCTTCTAAAG TCTTCTCCAG TGTGCAATGG CTCTCGGGTG TGAAGCCTGT TCTGAGCACA
    AAAAGCAAGT TCCGAGTGGT GGTGGAAGAG GTACAGGTGG TAGAACTAAA GGTTACTTGG
    ATCACTAAAA GCTTCTGTCC TGGAGGTACT GACAGTGTGA GTCCTCCACC GTCGATCATC
    AGCCAGGAGA ACCTGTCGAG GGTAAAGCGG CTGGGGTGCT TTGACCATGC CCAGAGGCAG
    CTTGGAGAAA GGTGCCTCTA TGTGTTCCCA GACAAAGTGG AGCCTGCAAA AATCACCTGT
    GAATGCCCAG AGAGGAACTG TGTCCTGGGT GAAGGATCAG TTGCCAAAAA GGTGAGGAGA
    CTGCTGAAGA AGCAGATAGT GAAGATCATG TCGTGCTCCC CAGAGTCTCA GGGTACCACT
    GAAGCCCCGG ACAAAGAGCC TGCTCCAGCT GCTGACCAAA AGGCAGAAGA CCTTAAGCCT
    GGCTCAAAGT GTGAACTGGA GGACTCTTCC AACGGTGCAC CGAGCGCGGG GGAGAGGAAG
    GAGGAGCAGC CTGAAGAAAC AGGGAAGGAC AAAGGGGGAG CAGCAGTGAG ACAGCAGCAA
    CCTCCCTTTC TGCTGAAAGA TGAGGGATCG GCTGATTACA GGCTGCAGTC CATGGAGCAG
    GATGCTGATG ATGAGGCAGC AGATGACACG GATGACACGA GCTCTGTCAC CTCCTCCGCC
    AGCTCCACCG CCTCATCCCA GAGCGGCAGC GGCACCGGCC GCAAGAAGAG CATCCCTCTT
    TCCATCAAAA ACTTGAAGCG GAAGCACAAG AAGAAGAAGA CCAAGATCTC GAGAGAATTT
    AAGCCAGGAG ACAGGGTTGC AGTGGAGGTG GTGACCACGA TGACTTCGGC TGACGTGATG
    TGGCAGGATG GCACCGTGGA GACCAACATT CGTTCCAACG AGCTGATTCC AGTCCATCAT
    CTGGACAACA ATGAATTCTG CCCCGGGGAT TTCGTGGTGG ACAAAAGAGC TCAGAGCTCC
    CAGGACCCCG GTGTGTATGG AGTCGTGCAG TCTGGTGACC ATATCGGCCG TACCTGTGTG
    GTGAAGTGGT TCAAGCTAAA ATCCAGTGGA GATGATGTGG AGCTGATAGG GGAGGAAGAG
    GATGTGAGTG TGTACGACAT TGCTGACCAC CCAGACTTTC GCTTCCGCAC CACTGACATT
    GTGATTCGCA TCGGCAATTC GGATGGAGCC ACAGCTAACG AAGACGAGCC TTCGGTCGGA
    CAGGTGGCTC GCGTGGATGT CAGCAGTAAA GTGGAGGTGG TGTGGGCTGA TAACTCCAAG
    ACGATCATTC TGCCTCAGCA CTTATACAAC ATTGAGTCGG AGATCGAGGA GTCGGACTAT
    GACTCTGTCG ACGGCAGCAC CAGTGGGGCA TCCTCTGAGT GGGAGGATGA AAGCGACAGC
    TGGGAGACAG ACAACGGCCT CATGGAAGAC GACCACCCGA GGATTGAGGA GTTGGAGCCC
    GAGGAGCCGG CAGCAGAGGA GGTGAAAAAA GTGGAGGAAC AAGTCCAGGG AGCTGTGGCT
    ATGGCAGTAG CAGATCTTGC TGCAGAGAAA GCTGGCAAGG ACGGAGCCCC GAAGAGCTTC
    AGGGAACTAA AGGAAGCCAT CAAGATCTTG GAAAGCCTGA AGAATATGAC TGTGGAGCAG
    CTGCTGACTG GCTCTCCCAC CTCCCCAACT GTGGAGCTGG AAAAACCCAC TCGGGAGAAG
    AAATTCTTGG ACGATATTAA AAAGCTGCAG GAAAATCTCA AGAAAACCTT GGATAACGTG
    GCGATTGCAG AAGAGGAAAA GATGGAAGCC ATGGTGGAGA CAGAGAAGAA AGAGGAAAAA
    GCAGAGGCAC AGACACCGGT GAGGTCGGAG TGGCCCAGCG AGACACCAGT GCTGTGCCAG
    CAGAGTGGGG GCAAACCTGG AGTCACCTTC ACCAGTGCCA AGGGAGAGGT GTTCTCTGTG
    CTGGAGTGTG CTCCAGATAG CCATGCCTTC AAGAAAATGG AGTTCCAGCC CCCAGAAGCA
    AAGAAGTTCT TTAGCACTGT GCGGAAGGAG ATGGCCCTGC TGGCCACCTC CCTGCCAGAT
    GGCATCATGG TTAAAACCTT TGAGGATCGA ATGGACCTCT TCTCAGCACT TATAAAAGGG
    CCCACACGCA CACCCTATGA GGACGGGCTC TTTCTCTTTG ACATCCAGCT CCCCAACATC
    TACCCTGCTG TGCCGCCTCT CTTTCGCTAC CTCTCCCAGT GCAGCGGGAG GCTGAATCCC
    AACCTGTATG ACAATGGCAA AGTGTGTGTC AGCCTCCTGG GGACGTGGAT AGGCAAGGGA
    ACAGAAAGGT GGACCAGTAA ATCCAGCCTC CTCCAAGTAC TCATCTCCAT CCAAGGTCTC
    ATCCTAGTGA ACGAGCCCTA TTACAACGAG GCGGGCTTCG ACAGCGACCG GGGCCTGCAG
    GAGGGCTACG AGAACAGCCG CTGCTACAAC GAGATGACGC TGATCCGGGT GGTGCAGTCC
    ATGATGCAGC TGCTGCGCAG ACCCGTGGAG GTCTTCGAGC ACGAAATCCG CGAGCATTTC
    CGCTGCAACG GCTGGCGCCT GGTGAGCCGC ATCGAGTCCT GGCTGGAGAC CAACGAGCTG
    GTGGAGAGGA GCCACGAGCA GAGCAACGGG GCAGATCCTG CCTCCCTCCT GTCTGCCGGC
    AGCGCCCTGG CCGACAGCCC CGGAGACACC GTGGGGTCGC CGGGGGAGTT GAGCGGCTCC
    GCCGAGCAGG GAGCGGCGGC CGAACTCTCC GACTCGGCGC TGGACGGGAA GGAGGAGTTG
    GACGAGGCGG AGTTCGCGAC CTCGGCGCCC AACGCCGGCG ACGTCCGCCA GCACTCGGAC
    TCGGAGGGCG CGGGCCAAGG CAGGGGGGTG GGCCTGGCCA GAGACCGAAC GGACGAGGGG
    AGGGCTGCCC AGGACAGTGC CTCGCAGCCG AGCGTGAAAC CAAAGAAAAG GAGAAAGAGC
    TACAGGAGTT TCCTTCCGGA GAAGAGCGGT TACCCCGATA TCGGGTTCCC CCTCTTCCCT
    TTGTCCAAGG GGTTCATTAA AAGCATCCGC GGTGTCTTGC AGCAGTACCG GGCTGCCTTA
    GCAGGGGCCA ATATCCCAGA GTGGACAGAG GACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa16521
    Clone ID Related Accession (Same CDS sequence) XM_415619.5 , XM_415619.6
    Accession Version XM_415619.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_415619.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATGTGC TTTCTGTGAA GGACACGGAT CTGCTGAAGC TTGAAGATCG CTCTGTGGTC 
    CCTCGAGACG TGGTCAGACA TATGAGCTCC AGTGACAGCC AGTGTGGGAC TGTAATTGAT
    GTCAACATAG AGTGTGCTGT GAAGCTGGTA GGAACCAACT GCATCCTGTA CCCTGTCAAC
    AGCAAAGACC TGCAACATAT CTGGCCCTTC ATGTATGGTG ACTACATAGC CTACGACTGC
    TGGCTGGGCA AGGTGTACGA TCTGAAGAAC CAAGTCATCC TTAAGCTTTC CAACGGAGCC
    AGGTGTTCAA TGAGCACAGA AGATGGAGCC AAGCTGTATG ACGTCTGTCC TCACGTCAGT
    GACTCGGGTC TCTTCTTTGA CGATTCATAT GGCTTTTATC CTGGGCAAGT CCTCATCGGG
    CCTTCTAAAG TCTTCTCCAG TGTGCAATGG CTCTCGGGTG TGAAGCCTGT TCTGAGCACA
    AAAAGCAAGT TCCGAGTGGT GGTGGAAGAG GTACAGGTGG TAGAACTAAA GGTTACTTGG
    ATCACTAAAA GCTTCTGTCC TGGAGGTACT GACAGTGTGA GTCCTCCACC GTCGATCATC
    AGCCAGGAGA ACCTGTCGAG GGTAAAGCGG CTGGGGTGCT TTGACCATGC CCAGAGGCAG
    CTTGGAGAAA GGTGCCTCTA TGTGTTCCCA GACAAAGTGG AGCCTGCAAA AATCACCTGT
    GAATGCCCAG AGAGGAACTG TGTCCTGGGT GAAGGATCAG TTGCCAAAAA GGTGAGGAGA
    CTGCTGAAGA AGCAGATAGT GAAGATCATG TCGTGCTCCC CAGAGTCTCA GGGTACCACT
    GAAGCCCCGG ACAAAGAGCC TGCTCCAGCT GCTGACCAAA AGGCAGAAGA CCTTAAGCCT
    GGCTCAAAGT GTGAACTGGA GGACTCTTCC AACGGTGCAC CGAGCGCGGG GGAGAGGAAG
    GAGGAGCAGC CTGAAGAAAC AGGGAAGGAC AAAGGGGGAG CAGCAGTGAG ACAGCAGCAA
    CCTCCCTTTC TGCTGAAAGA TGAGGGATCG GCTGATTACA GGCTGCAGTC CATGGAGCAG
    GATGCTGATG ATGAGGCAGC AGATGACACG GATGACACGA GCTCTGTCAC CTCCTCCGCC
    AGCTCCACCG CCTCATCCCA GAGCGGCAGC GGCACCGGCC GCAAGAAGAG CATCCCTCTT
    TCCATCAAAA ACTTGAAGCG GAAGCACAAG AAGAAGAAGA CCAAGATCTC GAGAGAATTT
    AAGCCAGGAG ACAGGGTTGC AGTGGAGGTG GTGACCACGA TGACTTCGGC TGACGTGATG
    TGGCAGGATG GCACCGTGGA GACCAACATT CGTTCCAACG AGCTGATTCC AGTCCATCAT
    CTGGACAACA ATGAATTCTG CCCCGGGGAT TTCGTGGTGG ACAAAAGAGC TCAGAGCTCC
    CAGGACCCCG GTGTGTATGG AGTCGTGCAG TCTGGTGACC ATATCGGCCG TACCTGTGTG
    GTGAAGTGGT TCAAGCTAAA ATCCAGTGGA GATGATGTGG AGCTGATAGG GGAGGAAGAG
    GATGTGAGTG TGTACGACAT TGCTGACCAC CCAGACTTTC GCTTCCGCAC CACTGACATT
    GTGATTCGCA TCGGCAATTC GGATGGAGCC ACAGCTAACG AAGACGAGCC TTCGGTCGGA
    CAGGTGGCTC GCGTGGATGT CAGCAGTAAA GTGGAGGTGG TGTGGGCTGA TAACTCCAAG
    ACGATCATTC TGCCTCAGCA CTTATACAAC ATTGAGTCGG AGATCGAGGA GTCGGACTAT
    GACTCTGTCG ACGGCAGCAC CAGTGGGGCA TCCTCTGAGT GGGAGGATGA AAGCGACAGC
    TGGGAGACAG ACAACGGCCT CATGGAAGAC GACCACCCGA GGATTGAGGA GTTGGAGCCC
    GAGGAGCCGG CAGCAGAGGA GGTGAAAAAA GTGGAGGAAC AAGTCCAGGG AGCTGTGGCT
    ATGGCAGTAG CAGATCTTGC TGCAGAGAAA GCTGGCAAGG ACGGAGCCCC GAAGAGCTTC
    AGGGAACTAA AGGAAGCCAT CAAGATCTTG GAAAGCCTGA AGAATATGAC TGTGGAGCAG
    CTGCTGACTG GCTCTCCCAC CTCCCCAACT GTGGAGCTGG AAAAACCCAC TCGGGAGAAG
    AAATTCTTGG ACGATATTAA AAAGCTGCAG GAAAATCTCA AGAAAACCTT GGATAACGTG
    GCGATTGCAG AAGAGGAAAA GATGGAAGCC ATGGTGGAGA CAGAGAAGAA AGAGGAAAAA
    GCAGAGGCAC AGACACCGGT GAGGTCGGAG TGGCCCAGCG AGACACCAGT GCTGTGCCAG
    CAGAGTGGGG GCAAACCTGG AGTCACCTTC ACCAGTGCCA AGGGAGAGGT GTTCTCTGTG
    CTGGAGTGTG CTCCAGATAG CCATGCCTTC AAGAAAATGG AGTTCCAGCC CCCAGAAGCA
    AAGAAGTTCT TTAGCACTGT GCGGAAGGAG ATGGCCCTGC TGGCCACCTC CCTGCCAGAT
    GGCATCATGG TTAAAACCTT TGAGGATCGA ATGGACCTCT TCTCAGCACT TATAAAAGGG
    CCCACACGCA CACCCTATGA GGACGGGCTC TTTCTCTTTG ACATCCAGCT CCCCAACATC
    TACCCTGCTG TGCCGCCTCT CTTTCGCTAC CTCTCCCAGT GCAGCGGGAG GCTGAATCCC
    AACCTGTATG ACAATGGCAA AGTGTGTGTC AGCCTCCTGG GGACGTGGAT AGGCAAGGGA
    ACAGAAAGGT GGACCAGTAA ATCCAGCCTC CTCCAAGTAC TCATCTCCAT CCAAGGTCTC
    ATCCTAGTGA ACGAGCCCTA TTACAACGAG GCGGGCTTCG ACAGCGACCG GGGCCTGCAG
    GAGGGCTACG AGAACAGCCG CTGCTACAAC GAGATGACGC TGATCCGGGT GGTGCAGTCC
    ATGATGCAGC TGCTGCGCAG ACCCGTGGAG GTCTTCGAGC ACGAAATCCG CGAGCATTTC
    CGCTGCAACG GCTGGCGCCT GGTGAGCCGC ATCGAGTCCT GGCTGGAGAC CAACGAGCTG
    GTGGAGAGGA GCCACGAGCA GAGCAACGGG GCAGATCCTG CCTCCCTCCT GTCTGCCGGC
    AGCGCCCTGG CCGACAGCCC CGGAGACACC GTGGGGTCGC CGGGGGAGTT GAGCGGCTCC
    GCCGAGCAGG GAGCGGCGGC CGAACTCTCC GACTCGGCGC TGGACGGGAA GGAGGAGTTG
    GACGAGGCGG AGTTCGCGAC CTCGGCGCCC AACGCCGGCG ACGTCCGCCA GCACTCGGAC
    TCGGAGGGCG CGGGCCAAGG CAGGGGGGTG GGCCTGGCCA GAGACCGAAC GGACGAGGGG
    AGGGCTGCCC AGGACAGTGC CTCGCAGCCG AGCGTGAAAC CAAAGAAAAG GAGAAAGAGC
    TACAGGAGTT TCCTTCCGGA GAAGAGCGGT TACCCCGATA TCGGGTTCCC CCTCTTCCCT
    TTGTCCAAGG GGTTCATTAA AAGCATCCGC GGTGTCTTGC AGCAGTACCG GGCTGCCTTA
    GCAGGGGCCA ATATCCCAGA GTGGACAGAG GACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_415619.4
    CDS102..3680
    Translation

    Target ORF information:

    RefSeq Version XM_415619.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_415619.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGTATGTGC TTTCTGTGAA GGACACGGAT CTGCTGAAGC TTGAAGATCG CTCTGTGGTC 
    CCTCGAGACG TGGTCAGACA TATGAGCTCC AGTGACAGCC AGTGTGGGAC TGTAATTGAT
    GTCAACATAG AGTGTGCTGT GAAGCTGGTA GGAACCAACT GCATCCTGTA CCCTGTCAAC
    AGCAAAGACC TGCAACATAT CTGGCCCTTC ATGTATGGTG ACTACATAGC CTACGACTGC
    TGGCTGGGCA AGGTGTACGA TCTGAAGAAC CAAGTCATCC TTAAGCTTTC CAACGGAGCC
    AGGTGTTCAA TGAGCACAGA AGATGGAGCC AAGCTGTATG ACGTCTGTCC TCACGTCAGT
    GACTCGGGTC TCTTCTTTGA CGATTCATAT GGCTTTTATC CTGGGCAAGT CCTCATCGGG
    CCTTCTAAAG TCTTCTCCAG TGTGCAATGG CTCTCGGGTG TGAAGCCTGT TCTGAGCACA
    AAAAGCAAGT TCCGAGTGGT GGTGGAAGAG GTACAGGTGG TAGAACTAAA GGTTACTTGG
    ATCACTAAAA GCTTCTGTCC TGGAGGTACT GACAGTGTGA GTCCTCCACC GTCGATCATC
    AGCCAGGAGA ACCTGTCGAG GGTAAAGCGG CTGGGGTGCT TTGACCATGC CCAGAGGCAG
    CTTGGAGAAA GGTGCCTCTA TGTGTTCCCA GACAAAGTGG AGCCTGCAAA AATCACCTGT
    GAATGCCCAG AGAGGAACTG TGTCCTGGGT GAAGGATCAG TTGCCAAAAA GGTGAGGAGA
    CTGCTGAAGA AGCAGATAGT GAAGATCATG TCGTGCTCCC CAGAGTCTCA GGGTACCACT
    GAAGCCCCGG ACAAAGAGCC TGCTCCAGCT GCTGACCAAA AGGCAGAAGA CCTTAAGCCT
    GGCTCAAAGT GTGAACTGGA GGACTCTTCC AACGGTGCAC CGAGCGCGGG GGAGAGGAAG
    GAGGAGCAGC CTGAAGAAAC AGGGAAGGAC AAAGGGGGAG CAGCAGTGAG ACAGCAGCAA
    CCTCCCTTTC TGCTGAAAGA TGAGGGATCG GCTGATTACA GGCTGCAGTC CATGGAGCAG
    GATGCTGATG ATGAGGCAGC AGATGACACG GATGACACGA GCTCTGTCAC CTCCTCCGCC
    AGCTCCACCG CCTCATCCCA GAGCGGCAGC GGCACCGGCC GCAAGAAGAG CATCCCTCTT
    TCCATCAAAA ACTTGAAGCG GAAGCACAAG AAGAAGAAGA CCAAGATCTC GAGAGAATTT
    AAGCCAGGAG ACAGGGTTGC AGTGGAGGTG GTGACCACGA TGACTTCGGC TGACGTGATG
    TGGCAGGATG GCACCGTGGA GACCAACATT CGTTCCAACG AGCTGATTCC AGTCCATCAT
    CTGGACAACA ATGAATTCTG CCCCGGGGAT TTCGTGGTGG ACAAAAGAGC TCAGAGCTCC
    CAGGACCCCG GTGTGTATGG AGTCGTGCAG TCTGGTGACC ATATCGGCCG TACCTGTGTG
    GTGAAGTGGT TCAAGCTAAA ATCCAGTGGA GATGATGTGG AGCTGATAGG GGAGGAAGAG
    GATGTGAGTG TGTACGACAT TGCTGACCAC CCAGACTTTC GCTTCCGCAC CACTGACATT
    GTGATTCGCA TCGGCAATTC GGATGGAGCC ACAGCTAACG AAGACGAGCC TTCGGTCGGA
    CAGGTGGCTC GCGTGGATGT CAGCAGTAAA GTGGAGGTGG TGTGGGCTGA TAACTCCAAG
    ACGATCATTC TGCCTCAGCA CTTATACAAC ATTGAGTCGG AGATCGAGGA GTCGGACTAT
    GACTCTGTCG ACGGCAGCAC CAGTGGGGCA TCCTCTGAGT GGGAGGATGA AAGCGACAGC
    TGGGAGACAG ACAACGGCCT CATGGAAGAC GACCACCCGA GGATTGAGGA GTTGGAGCCC
    GAGGAGCCGG CAGCAGAGGA GGTGAAAAAA GTGGAGGAAC AAGTCCAGGG AGCTGTGGCT
    ATGGCAGTAG CAGATCTTGC TGCAGAGAAA GCTGGCAAGG ACGGAGCCCC GAAGAGCTTC
    AGGGAACTAA AGGAAGCCAT CAAGATCTTG GAAAGCCTGA AGAATATGAC TGTGGAGCAG
    CTGCTGACTG GCTCTCCCAC CTCCCCAACT GTGGAGCTGG AAAAACCCAC TCGGGAGAAG
    AAATTCTTGG ACGATATTAA AAAGCTGCAG GAAAATCTCA AGAAAACCTT GGATAACGTG
    GCGATTGCAG AAGAGGAAAA GATGGAAGCC ATGGTGGAGA CAGAGAAGAA AGAGGAAAAA
    GCAGAGGCAC AGACACCGGT GAGGTCGGAG TGGCCCAGCG AGACACCAGT GCTGTGCCAG
    CAGAGTGGGG GCAAACCTGG AGTCACCTTC ACCAGTGCCA AGGGAGAGGT GTTCTCTGTG
    CTGGAGTGTG CTCCAGATAG CCATGCCTTC AAGAAAATGG AGTTCCAGCC CCCAGAAGCA
    AAGAAGTTCT TTAGCACTGT GCGGAAGGAG ATGGCCCTGC TGGCCACCTC CCTGCCAGAT
    GGCATCATGG TTAAAACCTT TGAGGATCGA ATGGACCTCT TCTCAGCACT TATAAAAGGG
    CCCACACGCA CACCCTATGA GGACGGGCTC TTTCTCTTTG ACATCCAGCT CCCCAACATC
    TACCCTGCTG TGCCGCCTCT CTTTCGCTAC CTCTCCCAGT GCAGCGGGAG GCTGAATCCC
    AACCTGTATG ACAATGGCAA AGTGTGTGTC AGCCTCCTGG GGACGTGGAT AGGCAAGGGA
    ACAGAAAGGT GGACCAGTAA ATCCAGCCTC CTCCAAGTAC TCATCTCCAT CCAAGGTCTC
    ATCCTAGTGA ACGAGCCCTA TTACAACGAG GCGGGCTTCG ACAGCGACCG GGGCCTGCAG
    GAGGGCTACG AGAACAGCCG CTGCTACAAC GAGATGACGC TGATCCGGGT GGTGCAGTCC
    ATGATGCAGC TGCTGCGCAG ACCCGTGGAG GTCTTCGAGC ACGAAATCCG CGAGCATTTC
    CGCTGCAACG GCTGGCGCCT GGTGAGCCGC ATCGAGTCCT GGCTGGAGAC CAACGAGCTG
    GTGGAGAGGA GCCACGAGCA GAGCAACGGG GCAGATCCTG CCTCCCTCCT GTCTGCCGGC
    AGCGCCCTGG CCGACAGCCC CGGAGACACC GTGGGGTCGC CGGGGGAGTT GAGCGGCTCC
    GCCGAGCAGG GAGCGGCGGC CGAACTCTCC GACTCGGCGC TGGACGGGAA GGAGGAGTTG
    GACGAGGCGG AGTTCGCGAC CTCGGCGCCC AACGCCGGCG ACGTCCGCCA GCACTCGGAC
    TCGGAGGGCG CGGGCCAAGG CAGGGGGGTG GGCCTGGCCA GAGACCGAAC GGACGAGGGG
    AGGGCTGCCC AGGACAGTGC CTCGCAGCCG AGCGTGAAAC CAAAGAAAAG GAGAAAGAGC
    TACAGGAGTT TCCTTCCGGA GAAGAGCGGT TACCCCGATA TCGGGTTCCC CCTCTTCCCT
    TTGTCCAAGG GGTTCATTAA AAGCATCCGC GGTGTCTTGC AGCAGTACCG GGCTGCCTTA
    GCAGGGGCCA ATATCCCAGA GTGGACAGAG GACAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43899
    Clone ID Related Accession (Same CDS sequence) XM_015295299.2 , XM_015295299.1
    Accession Version XM_015295299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006105.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015295299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGAGC TCCCCGCTGA GGCGGCTCCG GCCCCCGCCG CCCCGACCCC GGCCCCCGCT 
    CTGCCCTTAC CCCCGTCGGC GCCTCCGGGC GGTTCGCAGC GCCTCCTCTT CTCCCACGAC
    CTGGTGTCGG GTCGCTACCG CGGCTCGGTG CGTTTCGGCC TGGTTCGCCT CATCCACGGC
    GAAGACTCCG ATTCGGAGGA GGAGGAAACG GCGGCGGCGC CGGGGGGAGG CGGCCGGGGA
    GCGGGGGGAG CCGCCAGCTC GGGGGGCTCG GAGTCGGGCG GCCCCGGGGC GGGCGGCACG
    GAGGAGGGCC GCGCCAGTCC GCTGCGGCGG GGCTACGTGC GGGTGCAGTG GTACCCGGAG
    GGCATCAAAC AGCACGTCAA GGAGACCAAG CTGAAGCTTG AAGATCGCTC TGTGGTCCCT
    CGAGACGTGG TCAGACATAT GAGCTCCAGT GACAGCCAGT GTGGGACTGT AATTGATGTC
    AACATAGAGT GTGCTGTGAA GCTGGTAGGA ACCAACTGCA TCCTGTACCC TGTCAACAGC
    AAAGACCTGC AACATATCTG GCCCTTCATG TATGGTGACT ACATAGCCTA CGACTGCTGG
    CTGGGCAAGG TGTACGATCT GAAGAACCAA GTCATCCTTA AGCTTTCCAA CGGAGCCAGG
    TGTTCAATGA GCACAGAAGA TGGAGCCAAG CTGTATGACG TCTGTCCTCA CGTCAGTGAC
    TCGGGTCTCT TCTTTGACGA TTCATATGGC TTTTATCCTG GGCAAGTCCT CATCGGGCCT
    TCTAAAGTCT TCTCCAGTGT GCAATGGCTC TCGGGTGTGA AGCCTGTTCT GAGCACAAAA
    AGCAAGTTCC GAGTGGTGGT GGAAGAGGTA CAGGTGGTAG AACTAAAGGT TACTTGGATC
    ACTAAAAGCT TCTGTCCTGG AGGTACTGAC AGTGTGAGTC CTCCACCGTC GATCATCAGC
    CAGGAGAACC TGTCGAGGGT AAAGCGGCTG GGGTGCTTTG ACCATGCCCA GAGGCAGCTT
    GGAGAAAGGT GCCTCTATGT GTTCCCAGAC AAAGTGGAGC CTGCAAAAAT CACCTGTGAA
    TGCCCAGAGA GGAACTGTGT CCTGGGTGAA GGATCAGTTG CCAAAAAGGT GAGGAGACTG
    CTGAAGAAGC AGATAGTGAA GATCATGTCG TGCTCCCCAG AGTCTCAGGG TACCACTGAA
    GCCCCGGACA AAGAGCCTGC TCCAGCTGCT GACCAAAAGG CAGAAGACCT TAAGCCTGGC
    TCAAAGTGTG AACTGGAGGA CTCTTCCAAC GGTGCACCGA GCGCGGGGGA GAGGAAGGAG
    GAGCAGCCTG AAGAAACAGG GAAGGACAAA GGGGGAGCAG CAGTGAGACA GCAGCAACCT
    CCCTTTCTGC TGAAAGATGA GGGATCGGCT GATTACAGGC TGCAGTCCAT GGAGCAGGAT
    GCTGATGATG AGGCAGCAGA TGACACGGAT GACACGAGCT CTGTCACCTC CTCCGCCAGC
    TCCACCGCCT CATCCCAGAG CGGCAGCGGC ACCGGCCGCA AGAAGAGCAT CCCTCTTTCC
    ATCAAAAACT TGAAGCGGAA GCACAAGAAG AAGAAGACCA AGATCTCGAG AGAATTTAAG
    CCAGGAGACA GGGTTGCAGT GGAGGTGGTG ACCACGATGA CTTCGGCTGA CGTGATGTGG
    CAGGATGGCA CCGTGGAGAC CAACATTCGT TCCAACGAGC TGATTCCAGT CCATCATCTG
    GACAACAATG AATTCTGCCC CGGGGATTTC GTGGTGGACA AAAGAGCTCA GAGCTCCCAG
    GACCCCGGTG TGTATGGAGT CGTGCAGTCT GGTGACCATA TCGGCCGTAC CTGTGTGGTG
    AAGTGGTTCA AGCTAAAATC CAGTGGAGAT GATGTGGAGC TGATAGGGGA GGAAGAGGAT
    GTGAGTGTGT ACGACATTGC TGACCACCCA GACTTTCGCT TCCGCACCAC TGACATTGTG
    ATTCGCATCG GCAATTCGGA TGGAGCCACA GCTAACGAAG ACGAGCCTTC GGTCGGACAG
    GTGGCTCGCG TGGATGTCAG CAGTAAAGTG GAGGTGGTGT GGGCTGATAA CTCCAAGACG
    ATCATTCTGC CTCAGCACTT ATACAACATT GAGTCGGAGA TCGAGGAGTC GGACTATGAC
    TCTGTCGACG GCAGCACCAG TGGGGCATCC TCTGAGTGGG AGGATGAAAG CGACAGCTGG
    GAGACAGACA ACGGCCTCAT GGAAGACGAC CACCCGAGGA TTGAGGAGTT GGAGCCCGAG
    GAGCCGGCAG CAGAGGAGGT GAAAAAAGTG GAGGAACAAG TCCAGGGAGC TGTGGCTATG
    GCAGTAGCAG ATCTTGCTGC AGAGAAAGCT GGCAAGGACG GAGCCCCGAA GAGCTTCAGG
    GAACTAAAGG AAGCCATCAA GATCTTGGAA AGCCTGAAGA ATATGACTGT GGAGCAGCTG
    CTGACTGGCT CTCCCACCTC CCCAACTGTG GAGCTGGAAA AACCCACTCG GGAGAAGAAA
    TTCTTGGACG ATATTAAAAA GCTGCAGGAA AATCTCAAGA AAACCTTGGA TAACGTGGCG
    ATTGCAGAAG AGGAAAAGAT GGAAGCCATG GTGGAGACAG AGAAGAAAGA GGAAAAAGCA
    GAGGCACAGA CACCGGTGAG GTCGGAGTGG CCCAGCGAGA CACCAGTGCT GTGCCAGCAG
    AGTGGGGGCA AACCTGGAGT CACCTTCACC AGTGCCAAGG GAGAGGTGTT CTCTGTGCTG
    GAGTGTGCTC CAGATAGCCA TGCCTTCAAG AAAATGGAGT TCCAGCCCCC AGAAGCAAAG
    AAGTTCTTTA GCACTGTGCG GAAGGAGATG GCCCTGCTGG CCACCTCCCT GCCAGATGGC
    ATCATGGTTA AAACCTTTGA GGATCGAATG GACCTCTTCT CAGCACTTAT AAAAGGGCCC
    ACACGCACAC CCTATGAGGA CGGGCTCTTT CTCTTTGACA TCCAGCTCCC CAACATCTAC
    CCTGCTGTGC CGCCTCTCTT TCGCTACCTC TCCCAGTGCA GCGGGAGGCT GAATCCCAAC
    CTGTATGACA ATGGCAAAGT GTGTGTCAGC CTCCTGGGGA CGTGGATAGG CAAGGGAACA
    GAAAGGTGGA CCAGTAAATC CAGCCTCCTC CAAGTACTCA TCTCCATCCA AGGTCTCATC
    CTAGTGAACG AGCCCTATTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG
    GGCTACGAGA ACAGCCGCTG CTACAACGAG ATGACGCTGA TCCGGGTGGT GCAGTCCATG
    ATGCAGCTGC TGCGCAGACC CGTGGAGGTC TTCGAGCACG AAATCCGCGA GCATTTCCGC
    TGCAACGGCT GGCGCCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCAA CGAGCTGGTG
    GAGAGGAGCC ACGAGCAGAG CAACGGGGCA GATCCTGCCT CCCTCCTGTC TGCCGGCAGC
    GCCCTGGCCG ACAGCCCCGG AGACACCGTG GGGTCGCCGG GGGAGTTGAG CGGCTCCGCC
    GAGCAGGGAG CGGCGGCCGA ACTCTCCGAC TCGGCGCTGG ACGGGAAGGA GGAGTTGGAC
    GAGGCGGAGT TCGCGACCTC GGCGCCCAAC GCCGGCGACG TCCGCCAGCA CTCGGACTCG
    GAGGGCGCGG GCCAAGGCAG GGGGGTGGGC CTGGCCAGAG ACCGAACGGA CGAGGGGAGG
    GCTGCCCAGG ACAGTGCCTC GCAGCCGAGC GTGAAACCAA AGAAAAGGAG AAAGAGCTAC
    AGGAGTTTCC TTCCGGAGAA GAGCGGTTAC CCCGATATCG GGTTCCCCCT CTTCCCTTTG
    TCCAAGGGGT TCATTAAAAG CATCCGCGGT GTCTTGCAGC AGTACCGGGC TGCCTTAGCA
    GGGGCCAATA TCCCAGAGTG GACAGAGGAC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150785.1
    CDS51..3986
    Translation

    Target ORF information:

    RefSeq Version XM_015295299.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGAGC TCCCCGCTGA GGCGGCTCCG GCCCCCGCCG CCCCGACCCC GGCCCCCGCT 
    CTGCCCTTAC CCCCGTCGGC GCCTCCGGGC GGTTCGCAGC GCCTCCTCTT CTCCCACGAC
    CTGGTGTCGG GTCGCTACCG CGGCTCGGTG CGTTTCGGCC TGGTTCGCCT CATCCACGGC
    GAAGACTCCG ATTCGGAGGA GGAGGAAACG GCGGCGGCGC CGGGGGGAGG CGGCCGGGGA
    GCGGGGGGAG CCGCCAGCTC GGGGGGCTCG GAGTCGGGCG GCCCCGGGGC GGGCGGCACG
    GAGGAGGGCC GCGCCAGTCC GCTGCGGCGG GGCTACGTGC GGGTGCAGTG GTACCCGGAG
    GGCATCAAAC AGCACGTCAA GGAGACCAAG CTGAAGCTTG AAGATCGCTC TGTGGTCCCT
    CGAGACGTGG TCAGACATAT GAGCTCCAGT GACAGCCAGT GTGGGACTGT AATTGATGTC
    AACATAGAGT GTGCTGTGAA GCTGGTAGGA ACCAACTGCA TCCTGTACCC TGTCAACAGC
    AAAGACCTGC AACATATCTG GCCCTTCATG TATGGTGACT ACATAGCCTA CGACTGCTGG
    CTGGGCAAGG TGTACGATCT GAAGAACCAA GTCATCCTTA AGCTTTCCAA CGGAGCCAGG
    TGTTCAATGA GCACAGAAGA TGGAGCCAAG CTGTATGACG TCTGTCCTCA CGTCAGTGAC
    TCGGGTCTCT TCTTTGACGA TTCATATGGC TTTTATCCTG GGCAAGTCCT CATCGGGCCT
    TCTAAAGTCT TCTCCAGTGT GCAATGGCTC TCGGGTGTGA AGCCTGTTCT GAGCACAAAA
    AGCAAGTTCC GAGTGGTGGT GGAAGAGGTA CAGGTGGTAG AACTAAAGGT TACTTGGATC
    ACTAAAAGCT TCTGTCCTGG AGGTACTGAC AGTGTGAGTC CTCCACCGTC GATCATCAGC
    CAGGAGAACC TGTCGAGGGT AAAGCGGCTG GGGTGCTTTG ACCATGCCCA GAGGCAGCTT
    GGAGAAAGGT GCCTCTATGT GTTCCCAGAC AAAGTGGAGC CTGCAAAAAT CACCTGTGAA
    TGCCCAGAGA GGAACTGTGT CCTGGGTGAA GGATCAGTTG CCAAAAAGGT GAGGAGACTG
    CTGAAGAAGC AGATAGTGAA GATCATGTCG TGCTCCCCAG AGTCTCAGGG TACCACTGAA
    GCCCCGGACA AAGAGCCTGC TCCAGCTGCT GACCAAAAGG CAGAAGACCT TAAGCCTGGC
    TCAAAGTGTG AACTGGAGGA CTCTTCCAAC GGTGCACCGA GCGCGGGGGA GAGGAAGGAG
    GAGCAGCCTG AAGAAACAGG GAAGGACAAA GGGGGAGCAG CAGTGAGACA GCAGCAACCT
    CCCTTTCTGC TGAAAGATGA GGGATCGGCT GATTACAGGC TGCAGTCCAT GGAGCAGGAT
    GCTGATGATG AGGCAGCAGA TGACACGGAT GACACGAGCT CTGTCACCTC CTCCGCCAGC
    TCCACCGCCT CATCCCAGAG CGGCAGCGGC ACCGGCCGCA AGAAGAGCAT CCCTCTTTCC
    ATCAAAAACT TGAAGCGGAA GCACAAGAAG AAGAAGACCA AGATCTCGAG AGAATTTAAG
    CCAGGAGACA GGGTTGCAGT GGAGGTGGTG ACCACGATGA CTTCGGCTGA CGTGATGTGG
    CAGGATGGCA CCGTGGAGAC CAACATTCGT TCCAACGAGC TGATTCCAGT CCATCATCTG
    GACAACAATG AATTCTGCCC CGGGGATTTC GTGGTGGACA AAAGAGCTCA GAGCTCCCAG
    GACCCCGGTG TGTATGGAGT CGTGCAGTCT GGTGACCATA TCGGCCGTAC CTGTGTGGTG
    AAGTGGTTCA AGCTAAAATC CAGTGGAGAT GATGTGGAGC TGATAGGGGA GGAAGAGGAT
    GTGAGTGTGT ACGACATTGC TGACCACCCA GACTTTCGCT TCCGCACCAC TGACATTGTG
    ATTCGCATCG GCAATTCGGA TGGAGCCACA GCTAACGAAG ACGAGCCTTC GGTCGGACAG
    GTGGCTCGCG TGGATGTCAG CAGTAAAGTG GAGGTGGTGT GGGCTGATAA CTCCAAGACG
    ATCATTCTGC CTCAGCACTT ATACAACATT GAGTCGGAGA TCGAGGAGTC GGACTATGAC
    TCTGTCGACG GCAGCACCAG TGGGGCATCC TCTGAGTGGG AGGATGAAAG CGACAGCTGG
    GAGACAGACA ACGGCCTCAT GGAAGACGAC CACCCGAGGA TTGAGGAGTT GGAGCCCGAG
    GAGCCGGCAG CAGAGGAGGT GAAAAAAGTG GAGGAACAAG TCCAGGGAGC TGTGGCTATG
    GCAGTAGCAG ATCTTGCTGC AGAGAAAGCT GGCAAGGACG GAGCCCCGAA GAGCTTCAGG
    GAACTAAAGG AAGCCATCAA GATCTTGGAA AGCCTGAAGA ATATGACTGT GGAGCAGCTG
    CTGACTGGCT CTCCCACCTC CCCAACTGTG GAGCTGGAAA AACCCACTCG GGAGAAGAAA
    TTCTTGGACG ATATTAAAAA GCTGCAGGAA AATCTCAAGA AAACCTTGGA TAACGTGGCG
    ATTGCAGAAG AGGAAAAGAT GGAAGCCATG GTGGAGACAG AGAAGAAAGA GGAAAAAGCA
    GAGGCACAGA CACCGGTGAG GTCGGAGTGG CCCAGCGAGA CACCAGTGCT GTGCCAGCAG
    AGTGGGGGCA AACCTGGAGT CACCTTCACC AGTGCCAAGG GAGAGGTGTT CTCTGTGCTG
    GAGTGTGCTC CAGATAGCCA TGCCTTCAAG AAAATGGAGT TCCAGCCCCC AGAAGCAAAG
    AAGTTCTTTA GCACTGTGCG GAAGGAGATG GCCCTGCTGG CCACCTCCCT GCCAGATGGC
    ATCATGGTTA AAACCTTTGA GGATCGAATG GACCTCTTCT CAGCACTTAT AAAAGGGCCC
    ACACGCACAC CCTATGAGGA CGGGCTCTTT CTCTTTGACA TCCAGCTCCC CAACATCTAC
    CCTGCTGTGC CGCCTCTCTT TCGCTACCTC TCCCAGTGCA GCGGGAGGCT GAATCCCAAC
    CTGTATGACA ATGGCAAAGT GTGTGTCAGC CTCCTGGGGA CGTGGATAGG CAAGGGAACA
    GAAAGGTGGA CCAGTAAATC CAGCCTCCTC CAAGTACTCA TCTCCATCCA AGGTCTCATC
    CTAGTGAACG AGCCCTATTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG
    GGCTACGAGA ACAGCCGCTG CTACAACGAG ATGACGCTGA TCCGGGTGGT GCAGTCCATG
    ATGCAGCTGC TGCGCAGACC CGTGGAGGTC TTCGAGCACG AAATCCGCGA GCATTTCCGC
    TGCAACGGCT GGCGCCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCAA CGAGCTGGTG
    GAGAGGAGCC ACGAGCAGAG CAACGGGGCA GATCCTGCCT CCCTCCTGTC TGCCGGCAGC
    GCCCTGGCCG ACAGCCCCGG AGACACCGTG GGGTCGCCGG GGGAGTTGAG CGGCTCCGCC
    GAGCAGGGAG CGGCGGCCGA ACTCTCCGAC TCGGCGCTGG ACGGGAAGGA GGAGTTGGAC
    GAGGCGGAGT TCGCGACCTC GGCGCCCAAC GCCGGCGACG TCCGCCAGCA CTCGGACTCG
    GAGGGCGCGG GCCAAGGCAG GGGGGTGGGC CTGGCCAGAG ACCGAACGGA CGAGGGGAGG
    GCTGCCCAGG ACAGTGCCTC GCAGCCGAGC GTGAAACCAA AGAAAAGGAG AAAGAGCTAC
    AGGAGTTTCC TTCCGGAGAA GAGCGGTTAC CCCGATATCG GGTTCCCCCT CTTCCCTTTG
    TCCAAGGGGT TCATTAAAAG CATCCGCGGT GTCTTGCAGC AGTACCGGGC TGCCTTAGCA
    GGGGCCAATA TCCCAGAGTG GACAGAGGAC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa43899
    Clone ID Related Accession (Same CDS sequence) XM_015295299.2 , XM_015295299.1
    Accession Version XM_015295299.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product (E3-independent) E2 ubiquitin-conjugating enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455986.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGAGC TCCCCGCTGA GGCGGCTCCG GCCCCCGCCG CCCCGACCCC GGCCCCCGCT 
    CTGCCCTTAC CCCCGTCGGC GCCTCCGGGC GGTTCGCAGC GCCTCCTCTT CTCCCACGAC
    CTGGTGTCGG GTCGCTACCG CGGCTCGGTG CGTTTCGGCC TGGTTCGCCT CATCCACGGC
    GAAGACTCCG ATTCGGAGGA GGAGGAAACG GCGGCGGCGC CGGGGGGAGG CGGCCGGGGA
    GCGGGGGGAG CCGCCAGCTC GGGGGGCTCG GAGTCGGGCG GCCCCGGGGC GGGCGGCACG
    GAGGAGGGCC GCGCCAGTCC GCTGCGGCGG GGCTACGTGC GGGTGCAGTG GTACCCGGAG
    GGCATCAAAC AGCACGTCAA GGAGACCAAG CTGAAGCTTG AAGATCGCTC TGTGGTCCCT
    CGAGACGTGG TCAGACATAT GAGCTCCAGT GACAGCCAGT GTGGGACTGT AATTGATGTC
    AACATAGAGT GTGCTGTGAA GCTGGTAGGA ACCAACTGCA TCCTGTACCC TGTCAACAGC
    AAAGACCTGC AACATATCTG GCCCTTCATG TATGGTGACT ACATAGCCTA CGACTGCTGG
    CTGGGCAAGG TGTACGATCT GAAGAACCAA GTCATCCTTA AGCTTTCCAA CGGAGCCAGG
    TGTTCAATGA GCACAGAAGA TGGAGCCAAG CTGTATGACG TCTGTCCTCA CGTCAGTGAC
    TCGGGTCTCT TCTTTGACGA TTCATATGGC TTTTATCCTG GGCAAGTCCT CATCGGGCCT
    TCTAAAGTCT TCTCCAGTGT GCAATGGCTC TCGGGTGTGA AGCCTGTTCT GAGCACAAAA
    AGCAAGTTCC GAGTGGTGGT GGAAGAGGTA CAGGTGGTAG AACTAAAGGT TACTTGGATC
    ACTAAAAGCT TCTGTCCTGG AGGTACTGAC AGTGTGAGTC CTCCACCGTC GATCATCAGC
    CAGGAGAACC TGTCGAGGGT AAAGCGGCTG GGGTGCTTTG ACCATGCCCA GAGGCAGCTT
    GGAGAAAGGT GCCTCTATGT GTTCCCAGAC AAAGTGGAGC CTGCAAAAAT CACCTGTGAA
    TGCCCAGAGA GGAACTGTGT CCTGGGTGAA GGATCAGTTG CCAAAAAGGT GAGGAGACTG
    CTGAAGAAGC AGATAGTGAA GATCATGTCG TGCTCCCCAG AGTCTCAGGG TACCACTGAA
    GCCCCGGACA AAGAGCCTGC TCCAGCTGCT GACCAAAAGG CAGAAGACCT TAAGCCTGGC
    TCAAAGTGTG AACTGGAGGA CTCTTCCAAC GGTGCACCGA GCGCGGGGGA GAGGAAGGAG
    GAGCAGCCTG AAGAAACAGG GAAGGACAAA GGGGGAGCAG CAGTGAGACA GCAGCAACCT
    CCCTTTCTGC TGAAAGATGA GGGATCGGCT GATTACAGGC TGCAGTCCAT GGAGCAGGAT
    GCTGATGATG AGGCAGCAGA TGACACGGAT GACACGAGCT CTGTCACCTC CTCCGCCAGC
    TCCACCGCCT CATCCCAGAG CGGCAGCGGC ACCGGCCGCA AGAAGAGCAT CCCTCTTTCC
    ATCAAAAACT TGAAGCGGAA GCACAAGAAG AAGAAGACCA AGATCTCGAG AGAATTTAAG
    CCAGGAGACA GGGTTGCAGT GGAGGTGGTG ACCACGATGA CTTCGGCTGA CGTGATGTGG
    CAGGATGGCA CCGTGGAGAC CAACATTCGT TCCAACGAGC TGATTCCAGT CCATCATCTG
    GACAACAATG AATTCTGCCC CGGGGATTTC GTGGTGGACA AAAGAGCTCA GAGCTCCCAG
    GACCCCGGTG TGTATGGAGT CGTGCAGTCT GGTGACCATA TCGGCCGTAC CTGTGTGGTG
    AAGTGGTTCA AGCTAAAATC CAGTGGAGAT GATGTGGAGC TGATAGGGGA GGAAGAGGAT
    GTGAGTGTGT ACGACATTGC TGACCACCCA GACTTTCGCT TCCGCACCAC TGACATTGTG
    ATTCGCATCG GCAATTCGGA TGGAGCCACA GCTAACGAAG ACGAGCCTTC GGTCGGACAG
    GTGGCTCGCG TGGATGTCAG CAGTAAAGTG GAGGTGGTGT GGGCTGATAA CTCCAAGACG
    ATCATTCTGC CTCAGCACTT ATACAACATT GAGTCGGAGA TCGAGGAGTC GGACTATGAC
    TCTGTCGACG GCAGCACCAG TGGGGCATCC TCTGAGTGGG AGGATGAAAG CGACAGCTGG
    GAGACAGACA ACGGCCTCAT GGAAGACGAC CACCCGAGGA TTGAGGAGTT GGAGCCCGAG
    GAGCCGGCAG CAGAGGAGGT GAAAAAAGTG GAGGAACAAG TCCAGGGAGC TGTGGCTATG
    GCAGTAGCAG ATCTTGCTGC AGAGAAAGCT GGCAAGGACG GAGCCCCGAA GAGCTTCAGG
    GAACTAAAGG AAGCCATCAA GATCTTGGAA AGCCTGAAGA ATATGACTGT GGAGCAGCTG
    CTGACTGGCT CTCCCACCTC CCCAACTGTG GAGCTGGAAA AACCCACTCG GGAGAAGAAA
    TTCTTGGACG ATATTAAAAA GCTGCAGGAA AATCTCAAGA AAACCTTGGA TAACGTGGCG
    ATTGCAGAAG AGGAAAAGAT GGAAGCCATG GTGGAGACAG AGAAGAAAGA GGAAAAAGCA
    GAGGCACAGA CACCGGTGAG GTCGGAGTGG CCCAGCGAGA CACCAGTGCT GTGCCAGCAG
    AGTGGGGGCA AACCTGGAGT CACCTTCACC AGTGCCAAGG GAGAGGTGTT CTCTGTGCTG
    GAGTGTGCTC CAGATAGCCA TGCCTTCAAG AAAATGGAGT TCCAGCCCCC AGAAGCAAAG
    AAGTTCTTTA GCACTGTGCG GAAGGAGATG GCCCTGCTGG CCACCTCCCT GCCAGATGGC
    ATCATGGTTA AAACCTTTGA GGATCGAATG GACCTCTTCT CAGCACTTAT AAAAGGGCCC
    ACACGCACAC CCTATGAGGA CGGGCTCTTT CTCTTTGACA TCCAGCTCCC CAACATCTAC
    CCTGCTGTGC CGCCTCTCTT TCGCTACCTC TCCCAGTGCA GCGGGAGGCT GAATCCCAAC
    CTGTATGACA ATGGCAAAGT GTGTGTCAGC CTCCTGGGGA CGTGGATAGG CAAGGGAACA
    GAAAGGTGGA CCAGTAAATC CAGCCTCCTC CAAGTACTCA TCTCCATCCA AGGTCTCATC
    CTAGTGAACG AGCCCTATTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG
    GGCTACGAGA ACAGCCGCTG CTACAACGAG ATGACGCTGA TCCGGGTGGT GCAGTCCATG
    ATGCAGCTGC TGCGCAGACC CGTGGAGGTC TTCGAGCACG AAATCCGCGA GCATTTCCGC
    TGCAACGGCT GGCGCCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCAA CGAGCTGGTG
    GAGAGGAGCC ACGAGCAGAG CAACGGGGCA GATCCTGCCT CCCTCCTGTC TGCCGGCAGC
    GCCCTGGCCG ACAGCCCCGG AGACACCGTG GGGTCGCCGG GGGAGTTGAG CGGCTCCGCC
    GAGCAGGGAG CGGCGGCCGA ACTCTCCGAC TCGGCGCTGG ACGGGAAGGA GGAGTTGGAC
    GAGGCGGAGT TCGCGACCTC GGCGCCCAAC GCCGGCGACG TCCGCCAGCA CTCGGACTCG
    GAGGGCGCGG GCCAAGGCAG GGGGGTGGGC CTGGCCAGAG ACCGAACGGA CGAGGGGAGG
    GCTGCCCAGG ACAGTGCCTC GCAGCCGAGC GTGAAACCAA AGAAAAGGAG AAAGAGCTAC
    AGGAGTTTCC TTCCGGAGAA GAGCGGTTAC CCCGATATCG GGTTCCCCCT CTTCCCTTTG
    TCCAAGGGGT TCATTAAAAG CATCCGCGGT GTCTTGCAGC AGTACCGGGC TGCCTTAGCA
    GGGGCCAATA TCCCAGAGTG GACAGAGGAC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015150785.1
    CDS50..3985
    Translation

    Target ORF information:

    RefSeq Version XM_015295299.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus ubiquitin-conjugating enzyme E2O (UBE2O), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015295299.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGGAGC TCCCCGCTGA GGCGGCTCCG GCCCCCGCCG CCCCGACCCC GGCCCCCGCT 
    CTGCCCTTAC CCCCGTCGGC GCCTCCGGGC GGTTCGCAGC GCCTCCTCTT CTCCCACGAC
    CTGGTGTCGG GTCGCTACCG CGGCTCGGTG CGTTTCGGCC TGGTTCGCCT CATCCACGGC
    GAAGACTCCG ATTCGGAGGA GGAGGAAACG GCGGCGGCGC CGGGGGGAGG CGGCCGGGGA
    GCGGGGGGAG CCGCCAGCTC GGGGGGCTCG GAGTCGGGCG GCCCCGGGGC GGGCGGCACG
    GAGGAGGGCC GCGCCAGTCC GCTGCGGCGG GGCTACGTGC GGGTGCAGTG GTACCCGGAG
    GGCATCAAAC AGCACGTCAA GGAGACCAAG CTGAAGCTTG AAGATCGCTC TGTGGTCCCT
    CGAGACGTGG TCAGACATAT GAGCTCCAGT GACAGCCAGT GTGGGACTGT AATTGATGTC
    AACATAGAGT GTGCTGTGAA GCTGGTAGGA ACCAACTGCA TCCTGTACCC TGTCAACAGC
    AAAGACCTGC AACATATCTG GCCCTTCATG TATGGTGACT ACATAGCCTA CGACTGCTGG
    CTGGGCAAGG TGTACGATCT GAAGAACCAA GTCATCCTTA AGCTTTCCAA CGGAGCCAGG
    TGTTCAATGA GCACAGAAGA TGGAGCCAAG CTGTATGACG TCTGTCCTCA CGTCAGTGAC
    TCGGGTCTCT TCTTTGACGA TTCATATGGC TTTTATCCTG GGCAAGTCCT CATCGGGCCT
    TCTAAAGTCT TCTCCAGTGT GCAATGGCTC TCGGGTGTGA AGCCTGTTCT GAGCACAAAA
    AGCAAGTTCC GAGTGGTGGT GGAAGAGGTA CAGGTGGTAG AACTAAAGGT TACTTGGATC
    ACTAAAAGCT TCTGTCCTGG AGGTACTGAC AGTGTGAGTC CTCCACCGTC GATCATCAGC
    CAGGAGAACC TGTCGAGGGT AAAGCGGCTG GGGTGCTTTG ACCATGCCCA GAGGCAGCTT
    GGAGAAAGGT GCCTCTATGT GTTCCCAGAC AAAGTGGAGC CTGCAAAAAT CACCTGTGAA
    TGCCCAGAGA GGAACTGTGT CCTGGGTGAA GGATCAGTTG CCAAAAAGGT GAGGAGACTG
    CTGAAGAAGC AGATAGTGAA GATCATGTCG TGCTCCCCAG AGTCTCAGGG TACCACTGAA
    GCCCCGGACA AAGAGCCTGC TCCAGCTGCT GACCAAAAGG CAGAAGACCT TAAGCCTGGC
    TCAAAGTGTG AACTGGAGGA CTCTTCCAAC GGTGCACCGA GCGCGGGGGA GAGGAAGGAG
    GAGCAGCCTG AAGAAACAGG GAAGGACAAA GGGGGAGCAG CAGTGAGACA GCAGCAACCT
    CCCTTTCTGC TGAAAGATGA GGGATCGGCT GATTACAGGC TGCAGTCCAT GGAGCAGGAT
    GCTGATGATG AGGCAGCAGA TGACACGGAT GACACGAGCT CTGTCACCTC CTCCGCCAGC
    TCCACCGCCT CATCCCAGAG CGGCAGCGGC ACCGGCCGCA AGAAGAGCAT CCCTCTTTCC
    ATCAAAAACT TGAAGCGGAA GCACAAGAAG AAGAAGACCA AGATCTCGAG AGAATTTAAG
    CCAGGAGACA GGGTTGCAGT GGAGGTGGTG ACCACGATGA CTTCGGCTGA CGTGATGTGG
    CAGGATGGCA CCGTGGAGAC CAACATTCGT TCCAACGAGC TGATTCCAGT CCATCATCTG
    GACAACAATG AATTCTGCCC CGGGGATTTC GTGGTGGACA AAAGAGCTCA GAGCTCCCAG
    GACCCCGGTG TGTATGGAGT CGTGCAGTCT GGTGACCATA TCGGCCGTAC CTGTGTGGTG
    AAGTGGTTCA AGCTAAAATC CAGTGGAGAT GATGTGGAGC TGATAGGGGA GGAAGAGGAT
    GTGAGTGTGT ACGACATTGC TGACCACCCA GACTTTCGCT TCCGCACCAC TGACATTGTG
    ATTCGCATCG GCAATTCGGA TGGAGCCACA GCTAACGAAG ACGAGCCTTC GGTCGGACAG
    GTGGCTCGCG TGGATGTCAG CAGTAAAGTG GAGGTGGTGT GGGCTGATAA CTCCAAGACG
    ATCATTCTGC CTCAGCACTT ATACAACATT GAGTCGGAGA TCGAGGAGTC GGACTATGAC
    TCTGTCGACG GCAGCACCAG TGGGGCATCC TCTGAGTGGG AGGATGAAAG CGACAGCTGG
    GAGACAGACA ACGGCCTCAT GGAAGACGAC CACCCGAGGA TTGAGGAGTT GGAGCCCGAG
    GAGCCGGCAG CAGAGGAGGT GAAAAAAGTG GAGGAACAAG TCCAGGGAGC TGTGGCTATG
    GCAGTAGCAG ATCTTGCTGC AGAGAAAGCT GGCAAGGACG GAGCCCCGAA GAGCTTCAGG
    GAACTAAAGG AAGCCATCAA GATCTTGGAA AGCCTGAAGA ATATGACTGT GGAGCAGCTG
    CTGACTGGCT CTCCCACCTC CCCAACTGTG GAGCTGGAAA AACCCACTCG GGAGAAGAAA
    TTCTTGGACG ATATTAAAAA GCTGCAGGAA AATCTCAAGA AAACCTTGGA TAACGTGGCG
    ATTGCAGAAG AGGAAAAGAT GGAAGCCATG GTGGAGACAG AGAAGAAAGA GGAAAAAGCA
    GAGGCACAGA CACCGGTGAG GTCGGAGTGG CCCAGCGAGA CACCAGTGCT GTGCCAGCAG
    AGTGGGGGCA AACCTGGAGT CACCTTCACC AGTGCCAAGG GAGAGGTGTT CTCTGTGCTG
    GAGTGTGCTC CAGATAGCCA TGCCTTCAAG AAAATGGAGT TCCAGCCCCC AGAAGCAAAG
    AAGTTCTTTA GCACTGTGCG GAAGGAGATG GCCCTGCTGG CCACCTCCCT GCCAGATGGC
    ATCATGGTTA AAACCTTTGA GGATCGAATG GACCTCTTCT CAGCACTTAT AAAAGGGCCC
    ACACGCACAC CCTATGAGGA CGGGCTCTTT CTCTTTGACA TCCAGCTCCC CAACATCTAC
    CCTGCTGTGC CGCCTCTCTT TCGCTACCTC TCCCAGTGCA GCGGGAGGCT GAATCCCAAC
    CTGTATGACA ATGGCAAAGT GTGTGTCAGC CTCCTGGGGA CGTGGATAGG CAAGGGAACA
    GAAAGGTGGA CCAGTAAATC CAGCCTCCTC CAAGTACTCA TCTCCATCCA AGGTCTCATC
    CTAGTGAACG AGCCCTATTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG
    GGCTACGAGA ACAGCCGCTG CTACAACGAG ATGACGCTGA TCCGGGTGGT GCAGTCCATG
    ATGCAGCTGC TGCGCAGACC CGTGGAGGTC TTCGAGCACG AAATCCGCGA GCATTTCCGC
    TGCAACGGCT GGCGCCTGGT GAGCCGCATC GAGTCCTGGC TGGAGACCAA CGAGCTGGTG
    GAGAGGAGCC ACGAGCAGAG CAACGGGGCA GATCCTGCCT CCCTCCTGTC TGCCGGCAGC
    GCCCTGGCCG ACAGCCCCGG AGACACCGTG GGGTCGCCGG GGGAGTTGAG CGGCTCCGCC
    GAGCAGGGAG CGGCGGCCGA ACTCTCCGAC TCGGCGCTGG ACGGGAAGGA GGAGTTGGAC
    GAGGCGGAGT TCGCGACCTC GGCGCCCAAC GCCGGCGACG TCCGCCAGCA CTCGGACTCG
    GAGGGCGCGG GCCAAGGCAG GGGGGTGGGC CTGGCCAGAG ACCGAACGGA CGAGGGGAGG
    GCTGCCCAGG ACAGTGCCTC GCAGCCGAGC GTGAAACCAA AGAAAAGGAG AAAGAGCTAC
    AGGAGTTTCC TTCCGGAGAA GAGCGGTTAC CCCGATATCG GGTTCCCCCT CTTCCCTTTG
    TCCAAGGGGT TCATTAAAAG CATCCGCGGT GTCTTGCAGC AGTACCGGGC TGCCTTAGCA
    GGGGCCAATA TCCCAGAGTG GACAGAGGAC AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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