NUP153 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP153
Entrez Gene ID	106887404
Full Name	nucleoporin 153
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014940890.1	XP_014796376.1	nuclear pore complex protein Nup153 isoform X2
XM_014940893.1	XP_014796379.1	nuclear pore complex protein Nup153 isoform X5
XM_014940889.1	XP_014796375.1	nuclear pore complex protein Nup153 isoform X1
XM_014940894.1	XP_014796380.1	nuclear pore complex protein Nup153 isoform X6
XM_014940891.1	XP_014796377.1	nuclear pore complex protein Nup153 isoform X3
XM_014940892.1	XP_014796378.1	nuclear pore complex protein Nup153 isoform X4

Pathways from BioSystems
Gene Ontology

The following NUP153 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP153 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa64254	XM_014940893.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCa64250	XM_014940889.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCa64251	XM_014940890.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCa64252	XM_014940891.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCa64253	XM_014940892.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCa64255	XM_014940894.1 Latest version!	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP153 ( XM_014940893.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796379.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCa64254
Clone ID Related Accession (Same CDS sequence)	XM_014940893.1
Accession Version	XM_014940893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA TCATTAGCAG AGTTACAGAA TCGGTGAAAA ATATTGTACC GGGATGGTTA 
CAGAAGTATT TCAATAGAAG TGAAGATGAA TGTGTAGATA CAAATGAATC TACAAACCAG 
GAAGAAAGTC CAGTAAACTA TCACCATGAT TATGCAAATG AAGATACCAT AGTGATTGAT 
GGGAGAGTCA CGCCTGAATC AGCAAGAATT AATTTAGAAG AACCATCCAC GAGTAGATCT 
GCATTGAACT TCTCAGATGT GTTAACAAGG CCCTCTCTTC ATCGGAGTCA TTTGAATTGT 
ACCATGTTGG ATTCCACAGT ACCACATTGT CAGCCCTCTA CATCTTCTTC ACTTGGCATT 
GGCAGTCCTG GACTGTCTCT TGTAAAAGAA ATAAAAGATT CTACCTCTCA GCATGATGAC 
GATAACATCT CAACAACCAG TGGCTTCTCC TCTAGAGCAT CTGATAAAGA TATCACAGTT 
TCAAAAAATA CTTCAGCGCC GCTGCTCTGG TCCACAGAGG CTGAAAGATC TCATTCCCTC 
TCACAGCATT CTGCAACTAG CTCTAAAAAA CCTGCATTCA ATTTATCTGC TTTTGGATCT 
CCCTCTCCTT CACTTGGAAA CACTTCTGTC TTTAAGACAA GCCAGCTTGG GGATTCTCCA 
TTTTACCCTG GAAAGACGAC GTACGGTGGT GCAGCTGCAG CAGCAAGGGA GACAAAAGCA 
CGAATTGCTC CTTATCAGCC CCCGGTAAGA AGACAAGTGA AAGCTAAACA AGCAAGTGTG 
CAATCCTATG GTGTGACAAG TTCCACAGCG CGGCGCATCT TGCAGTCATT GGAGAAGATG 
TCAAGTCCTT TGGCGGATGC TAAAAGGATT CCATCTTCTG TTTCTAGTCC ACTGTCTTCT 
CCTGTGGACA GGGGTGCACA GCTGAATATT GCTAGCTTCC AATCCAAACG AAAACAGATG 
GAATCGCAGC ATCCACCTGT CCAGAAACTC GTGACACCAA AGGCAATCTC TCTGTCAGTG 
AGTCGGACCC AGTATTTTAA ACCATCTCTG ACTCCAGCTA CTGATTCCAG CAAAATTCAA 
CAGAGGGTAG ATATAAAGCA CAAAGGCATG AGAGAGAAGA GTTTTCCTGC AGAACAGCAA 
GTAAAACCAT CTGAAAGCAA TTTGACCTAC CCTAAATTCA GTAGTCCTGC ATCCAATGGT 
CTGTCTTCAG GAGGAGTAGG TGGTGGTGGC AAGATGAGAA GGGAAAGAGG AGTACACTAT 
ATATCAAAAC CTGGACAAGA ACAAGAGGAG GTGGAAGAAC CGGTACTACC GAAAGTACCC 
CTACCCATCA GTACTGCATC GCTGCCTTCG TTCAACTTTA GTTTTCTTCC CAGTAGTACT 
GTCTCATCCT CACCAAGCAC TGTTTCAACA GCAGTGATGA ACAAGGTACA ACCAGCAAGC 
AATGTTGGCA GTCCTGTCTT CAGATTTTCA TCTCCAATTG TGAAATCTAC TGAGGCGGAA 
GTGCTACCTC CGTTGTCTCA GATTGGGTTT ACATTTAGTG TTCCTGTTGT AAAATCAGCA 
GAGCTTTCTG GATCCAGCGA TACACCAGTG ACGTCCTTAC TTACTCTAGA TACCACCACT 
GTAAACAGCA CCAGCAATAA AAAGGAAGAA AAAGAAGAAT ATGATGGCCT CACCAAGCCT 
GCAAAAGTCT TAAAGGAAGG AAGTGTGTTA GACATTCTAA AAAGCCCTGG CTTCACGTCT 
GAGAAAACAC ACTCCTCTAC ATCCGCACAG CCTGTTACGA GCACAGTGGT CTATACAAGG 
CCTGCAATAA GTAGTTTTTC TGCAGGCAAA GACAGCCCTA AACAGCCATC CTCGTTTTGG 
CAGTCTGACA CGCATGACCC ATGTCTGCAA AACAAACCCA CAGATGGCAA ATGTGTAACG 
TGTCAAGCTG CTAAAGTGTC AACAGTGGAG AGTACGAAAC CCACGATAAG CTCTAGTCCG 
TGCGTCACCT CGAAGGCCGC AGTCCCTACA GCCGGGACAC TGGGCTTTGA AGACAAATTT 
AAACCACCAC CCAACACATG GGATTGTGAT ACCTGTCTGG TCCAGAACAA ACCTGAAGCT 
ACAAAATGTA TAGCCTGTGA GACACCAAAG CCTGGGACAG GAGTAATGCC TGCTCTGACA 
TTGCCAGTGG TCACAGACAG CTCGGTGACA GTGACATCCT CCTCCAGCAG CACTGACACA 
ACGGCCACTT TGGGATTTGG AGACAAATTT AAGAAGCCAA AAGGCTCTTG GGAGTGTGCA 
GTGTGCCTTG TATCAAATAA GGCAGAAGAC AGCAAATGTG TAGCCTGTCA GTCTGAGAAA 
CCAGGGAGTT CAGTGCCTGT GACCAGTAGC AGTGTTTCTA CATTTTCTGC TCCTTCAGGA 
GGATTTCTGG ATTTAGACAA ATTCAAGAAA CCTGAAGGAA GCTGGGATTG TGAGGTCTGC 
TTGGTCCAAA ACAAAGCAGA AGCCACAAAA TGTGTAGCCT GTGAAAGTGC AAAGCCAGGC 
ACCAAAGCAG AGCTCAAAGG TTTTGGTACT CCTACTGTGT CCACAAATGC TGCAATGCCA 
TCATTTACAT TTGGTGTCCA GTCGTCCTCC TCTGAATCTT CTCATATGTT AGGTAGCACA 
GGAAATTTTA AATTTGGAGA ACAAGGGAGC TTCAAGTTTG GCGTTGCATC AGAACCTGCG 
TCCTCCAACA CTGTGACTGG GGGTTTTAAG TTTCCTACTA GTTCAGGGGA CTTCAAATTT 
GGAGTTTCTT CCTCAGACTC TAAATCAGAA GACAGTAAAA AAGAAGGTAA AAGTAACAGT 
TTTACCTTTG GACTTCCATC TACAAGCAGT CAGGCTCCTT CAACATTTCA GTTTGGGACA 
GCGAGCCTGG GACAGCAGGA AAAGAAAGAG GAACCAGTTT TGGGAGGCTT TGGTTTTGGC 
ACGAGTTCTA CTTCTTCCAT AGCTACCAGT GAGAATAAAA CCGGAGTCAG TGGCTTCACT 
TTTGGAACTG TGGCAGAAAA GGAGGTAGCA TCACCTGCTC TTACATTTAA GAAGTCAGAT 
GAGAAAAAGG ATGAAACTCT TTCAACGAAG GGAGGCTTCT CTTTTGGCAA CGTGGAGTCT 
GCACCTGCCT CACAGTTTGT TTTGGGAAGG ACAGAAGAGA AACAGGACTT GGTCACTTCT 
GCTGCTCCGT TAGCATTTGG AAAGAAAGCT GACAGTGAAG AGTTAAAGGC ACAACCTGTC 
TTTTCAGCTG GAACTGCTGA GCATACCAAA GAGGAGAGCG CAGCAAAACC TATATTCAAC 
TTCAGTTTCG TCAAACCATC GGAGAAGGAA ACCGATCAGG CAAAGCCGTC TTTTGCGTTT 
GGGGCACAAA CCAGTACTTC AGATCAAGGA GCAGCAAAGC CATCTTTCAG TTTCTTGAGC 
TCAAGTTCCT CCAGCACAGT CGTACCCACC ACTTCAGCCA ACAGCAGCAG CGTGTTTGGC 
AGTGCCATCT CTTCCTCAAA TCCTCAGCCA GTTCCCGCTC CCTTCGTGTT TGGACAACCC 
AGCAACACTG TGAGCAGCTC TGTTTTTGGC AATTCTGCAG AATCTACAGC ATCTCAGTCA 
TTTGGCTTCT CTCAAGAAAA CAAACCAGCA ACCACATCTT CCAGCACCGG CACGGCTCTT 
GCTTCATTTC TCTTTGGTTC GGGAGCCAGC AGTACCAATG CAGCAAGTTC AGGCTTTACC 
TTTGGAGCCA CAACCACATC AAGTTCAACA GGGTCATCTT CCTCCTTTCT GTTTGGCTCC 
GGGCCCCCGG CGCCTGCAGC CGGCCCCGCG TTTGGCGCCA GCCAGCCACC AGCATTTGGC 
CAGAGCCAAG GCTCCGGCCA GCCCAGCGCT CCCAGTTTCG GATCGCTGTC GACGACGCTG 
TTCTCAGCTG GCGCCCAGTC TGCTCCTCCC GCCTTCGGCT CGGTGACCAG CAGCACCCAG 
CCCTCCGTCT TCGGACAGCA AGCCACCCAA CAGCCAGGGT TTGGCTCTGC TACCCCCAGC 
ACTGGTTCCG TATTCCAGTT TGGAAGTAGT ACTTCTAATT TCAACTTTCC GAGTAACCCC 
GGCGTATTTA CTTTTGGTGC AAATACTCCC GCACCGGCAG CACCGGCACA GCCTTCTGGC 
TCGTCAGGAT TTTCCTTCAA CCAGCCTCTG GCATTCACAG TGGGGACTAA TGGAAAAAAT 
GTTTTCTCTG CCTCCGGATC TTCAGTTTCT GGTCGGAAGA TAAAAACTGC TGTTAGACGT 
AGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796379.1
CDS	16..4284
Translation	MEGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPSLGNTSVFKTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQMESQHPPVQKLVTPKAISLSVSRTQYFKPSLTPATDSSKIQQRVDIKHKGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSQIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940893.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940893.1

ORF Insert Sequence:

ATGGAGGGAA TCATTAGCAG AGTTACAGAA TCGGTGAAAA ATATTGTACC GGGATGGTTA 
CAGAAGTATT TCAATAGAAG TGAAGATGAA TGTGTAGATA CAAATGAATC TACAAACCAG 
GAAGAAAGTC CAGTAAACTA TCACCATGAT TATGCAAATG AAGATACCAT AGTGATTGAT 
GGGAGAGTCA CGCCTGAATC AGCAAGAATT AATTTAGAAG AACCATCCAC GAGTAGATCT 
GCATTGAACT TCTCAGATGT GTTAACAAGG CCCTCTCTTC ATCGGAGTCA TTTGAATTGT 
ACCATGTTGG ATTCCACAGT ACCACATTGT CAGCCCTCTA CATCTTCTTC ACTTGGCATT 
GGCAGTCCTG GACTGTCTCT TGTAAAAGAA ATAAAAGATT CTACCTCTCA GCATGATGAC 
GATAACATCT CAACAACCAG TGGCTTCTCC TCTAGAGCAT CTGATAAAGA TATCACAGTT 
TCAAAAAATA CTTCAGCGCC GCTGCTCTGG TCCACAGAGG CTGAAAGATC TCATTCCCTC 
TCACAGCATT CTGCAACTAG CTCTAAAAAA CCTGCATTCA ATTTATCTGC TTTTGGATCT 
CCCTCTCCTT CACTTGGAAA CACTTCTGTC TTTAAGACAA GCCAGCTTGG GGATTCTCCA 
TTTTACCCTG GAAAGACGAC GTACGGTGGT GCAGCTGCAG CAGCAAGGGA GACAAAAGCA 
CGAATTGCTC CTTATCAGCC CCCGGTAAGA AGACAAGTGA AAGCTAAACA AGCAAGTGTG 
CAATCCTATG GTGTGACAAG TTCCACAGCG CGGCGCATCT TGCAGTCATT GGAGAAGATG 
TCAAGTCCTT TGGCGGATGC TAAAAGGATT CCATCTTCTG TTTCTAGTCC ACTGTCTTCT 
CCTGTGGACA GGGGTGCACA GCTGAATATT GCTAGCTTCC AATCCAAACG AAAACAGATG 
GAATCGCAGC ATCCACCTGT CCAGAAACTC GTGACACCAA AGGCAATCTC TCTGTCAGTG 
AGTCGGACCC AGTATTTTAA ACCATCTCTG ACTCCAGCTA CTGATTCCAG CAAAATTCAA 
CAGAGGGTAG ATATAAAGCA CAAAGGCATG AGAGAGAAGA GTTTTCCTGC AGAACAGCAA 
GTAAAACCAT CTGAAAGCAA TTTGACCTAC CCTAAATTCA GTAGTCCTGC ATCCAATGGT 
CTGTCTTCAG GAGGAGTAGG TGGTGGTGGC AAGATGAGAA GGGAAAGAGG AGTACACTAT 
ATATCAAAAC CTGGACAAGA ACAAGAGGAG GTGGAAGAAC CGGTACTACC GAAAGTACCC 
CTACCCATCA GTACTGCATC GCTGCCTTCG TTCAACTTTA GTTTTCTTCC CAGTAGTACT 
GTCTCATCCT CACCAAGCAC TGTTTCAACA GCAGTGATGA ACAAGGTACA ACCAGCAAGC 
AATGTTGGCA GTCCTGTCTT CAGATTTTCA TCTCCAATTG TGAAATCTAC TGAGGCGGAA 
GTGCTACCTC CGTTGTCTCA GATTGGGTTT ACATTTAGTG TTCCTGTTGT AAAATCAGCA 
GAGCTTTCTG GATCCAGCGA TACACCAGTG ACGTCCTTAC TTACTCTAGA TACCACCACT 
GTAAACAGCA CCAGCAATAA AAAGGAAGAA AAAGAAGAAT ATGATGGCCT CACCAAGCCT 
GCAAAAGTCT TAAAGGAAGG AAGTGTGTTA GACATTCTAA AAAGCCCTGG CTTCACGTCT 
GAGAAAACAC ACTCCTCTAC ATCCGCACAG CCTGTTACGA GCACAGTGGT CTATACAAGG 
CCTGCAATAA GTAGTTTTTC TGCAGGCAAA GACAGCCCTA AACAGCCATC CTCGTTTTGG 
CAGTCTGACA CGCATGACCC ATGTCTGCAA AACAAACCCA CAGATGGCAA ATGTGTAACG 
TGTCAAGCTG CTAAAGTGTC AACAGTGGAG AGTACGAAAC CCACGATAAG CTCTAGTCCG 
TGCGTCACCT CGAAGGCCGC AGTCCCTACA GCCGGGACAC TGGGCTTTGA AGACAAATTT 
AAACCACCAC CCAACACATG GGATTGTGAT ACCTGTCTGG TCCAGAACAA ACCTGAAGCT 
ACAAAATGTA TAGCCTGTGA GACACCAAAG CCTGGGACAG GAGTAATGCC TGCTCTGACA 
TTGCCAGTGG TCACAGACAG CTCGGTGACA GTGACATCCT CCTCCAGCAG CACTGACACA 
ACGGCCACTT TGGGATTTGG AGACAAATTT AAGAAGCCAA AAGGCTCTTG GGAGTGTGCA 
GTGTGCCTTG TATCAAATAA GGCAGAAGAC AGCAAATGTG TAGCCTGTCA GTCTGAGAAA 
CCAGGGAGTT CAGTGCCTGT GACCAGTAGC AGTGTTTCTA CATTTTCTGC TCCTTCAGGA 
GGATTTCTGG ATTTAGACAA ATTCAAGAAA CCTGAAGGAA GCTGGGATTG TGAGGTCTGC 
TTGGTCCAAA ACAAAGCAGA AGCCACAAAA TGTGTAGCCT GTGAAAGTGC AAAGCCAGGC 
ACCAAAGCAG AGCTCAAAGG TTTTGGTACT CCTACTGTGT CCACAAATGC TGCAATGCCA 
TCATTTACAT TTGGTGTCCA GTCGTCCTCC TCTGAATCTT CTCATATGTT AGGTAGCACA 
GGAAATTTTA AATTTGGAGA ACAAGGGAGC TTCAAGTTTG GCGTTGCATC AGAACCTGCG 
TCCTCCAACA CTGTGACTGG GGGTTTTAAG TTTCCTACTA GTTCAGGGGA CTTCAAATTT 
GGAGTTTCTT CCTCAGACTC TAAATCAGAA GACAGTAAAA AAGAAGGTAA AAGTAACAGT 
TTTACCTTTG GACTTCCATC TACAAGCAGT CAGGCTCCTT CAACATTTCA GTTTGGGACA 
GCGAGCCTGG GACAGCAGGA AAAGAAAGAG GAACCAGTTT TGGGAGGCTT TGGTTTTGGC 
ACGAGTTCTA CTTCTTCCAT AGCTACCAGT GAGAATAAAA CCGGAGTCAG TGGCTTCACT 
TTTGGAACTG TGGCAGAAAA GGAGGTAGCA TCACCTGCTC TTACATTTAA GAAGTCAGAT 
GAGAAAAAGG ATGAAACTCT TTCAACGAAG GGAGGCTTCT CTTTTGGCAA CGTGGAGTCT 
GCACCTGCCT CACAGTTTGT TTTGGGAAGG ACAGAAGAGA AACAGGACTT GGTCACTTCT 
GCTGCTCCGT TAGCATTTGG AAAGAAAGCT GACAGTGAAG AGTTAAAGGC ACAACCTGTC 
TTTTCAGCTG GAACTGCTGA GCATACCAAA GAGGAGAGCG CAGCAAAACC TATATTCAAC 
TTCAGTTTCG TCAAACCATC GGAGAAGGAA ACCGATCAGG CAAAGCCGTC TTTTGCGTTT 
GGGGCACAAA CCAGTACTTC AGATCAAGGA GCAGCAAAGC CATCTTTCAG TTTCTTGAGC 
TCAAGTTCCT CCAGCACAGT CGTACCCACC ACTTCAGCCA ACAGCAGCAG CGTGTTTGGC 
AGTGCCATCT CTTCCTCAAA TCCTCAGCCA GTTCCCGCTC CCTTCGTGTT TGGACAACCC 
AGCAACACTG TGAGCAGCTC TGTTTTTGGC AATTCTGCAG AATCTACAGC ATCTCAGTCA 
TTTGGCTTCT CTCAAGAAAA CAAACCAGCA ACCACATCTT CCAGCACCGG CACGGCTCTT 
GCTTCATTTC TCTTTGGTTC GGGAGCCAGC AGTACCAATG CAGCAAGTTC AGGCTTTACC 
TTTGGAGCCA CAACCACATC AAGTTCAACA GGGTCATCTT CCTCCTTTCT GTTTGGCTCC 
GGGCCCCCGG CGCCTGCAGC CGGCCCCGCG TTTGGCGCCA GCCAGCCACC AGCATTTGGC 
CAGAGCCAAG GCTCCGGCCA GCCCAGCGCT CCCAGTTTCG GATCGCTGTC GACGACGCTG 
TTCTCAGCTG GCGCCCAGTC TGCTCCTCCC GCCTTCGGCT CGGTGACCAG CAGCACCCAG 
CCCTCCGTCT TCGGACAGCA AGCCACCCAA CAGCCAGGGT TTGGCTCTGC TACCCCCAGC 
ACTGGTTCCG TATTCCAGTT TGGAAGTAGT ACTTCTAATT TCAACTTTCC GAGTAACCCC 
GGCGTATTTA CTTTTGGTGC AAATACTCCC GCACCGGCAG CACCGGCACA GCCTTCTGGC 
TCGTCAGGAT TTTCCTTCAA CCAGCCTCTG GCATTCACAG TGGGGACTAA TGGAAAAAAT 
GTTTTCTCTG CCTCCGGATC TTCAGTTTCT GGTCGGAAGA TAAAAACTGC TGTTAGACGT 
AGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP153 ( XM_014940889.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796375.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa64250
Clone ID Related Accession (Same CDS sequence)	XM_014940889.1
Accession Version	XM_014940889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GATGGAATCG 
CAGCATCCAC CTGTCCAGAA ACTCGTGACA CCAAAGGCAA TCTCTCTGTC AGTGAGTCGG 
ACCCAGTATT TTAAACCATC TCTGACTCCA GCTACTGATT CCAGCAAAAT TCAACAGAGG 
GTAGATATAA AGCACAAAGG CATGAGAGAG AAGAGTTTTC CTGCAGAACA GCAAGTAAAA 
CCATCTGAAA GCAATTTGAC CTACCCTAAA TTCAGTAGTC CTGCATCCAA TGGTCTGTCT 
TCAGGAGGAG TAGGTGGTGG TGGCAAGATG AGAAGGGAAA GAGGAGTACA CTATATATCA 
AAACCTGGAC AAGAACAAGA GGAGGTGGAA GAACCGGTAC TACCGAAAGT ACCCCTACCC 
ATCAGTACTG CATCGCTGCC TTCGTTCAAC TTTAGTTTTC TTCCCAGTAG TACTGTCTCA 
TCCTCACCAA GCACTGTTTC AACAGCAGTG ATGAACAAGG TACAACCAGC AAGCAATGTT 
GGCAGTCCTG TCTTCAGATT TTCATCTCCA ATTGTGAAAT CTACTGAGGC GGAAGTGCTA 
CCTCCGTTGT CTCAGATTGG GTTTACATTT AGTGTTCCTG TTGTAAAATC AGCAGAGCTT 
TCTGGATCCA GCGATACACC AGTGACGTCC TTACTTACTC TAGATACCAC CACTGTAAAC 
AGCACCAGCA ATAAAAAGGA AGAAAAAGAA GAATATGATG GCCTCACCAA GCCTGCAAAA 
GTCTTAAAGG AAGGAAGTGT GTTAGACATT CTAAAAAGCC CTGGCTTCAC GTCTGAGAAA 
ACACACTCCT CTACATCCGC ACAGCCTGTT ACGAGCACAG TGGTCTATAC AAGGCCTGCA 
ATAAGTAGTT TTTCTGCAGG CAAAGACAGC CCTAAACAGC CATCCTCGTT TTGGCAGTCT 
GACACGCATG ACCCATGTCT GCAAAACAAA CCCACAGATG GCAAATGTGT AACGTGTCAA 
GCTGCTAAAG TGTCAACAGT GGAGAGTACG AAACCCACGA TAAGCTCTAG TCCGTGCGTC 
ACCTCGAAGG CCGCAGTCCC TACAGCCGGG ACACTGGGCT TTGAAGACAA ATTTAAACCA 
CCACCCAACA CATGGGATTG TGATACCTGT CTGGTCCAGA ACAAACCTGA AGCTACAAAA 
TGTATAGCCT GTGAGACACC AAAGCCTGGG ACAGGAGTAA TGCCTGCTCT GACATTGCCA 
GTGGTCACAG ACAGCTCGGT GACAGTGACA TCCTCCTCCA GCAGCACTGA CACAACGGCC 
ACTTTGGGAT TTGGAGACAA ATTTAAGAAG CCAAAAGGCT CTTGGGAGTG TGCAGTGTGC 
CTTGTATCAA ATAAGGCAGA AGACAGCAAA TGTGTAGCCT GTCAGTCTGA GAAACCAGGG 
AGTTCAGTGC CTGTGACCAG TAGCAGTGTT TCTACATTTT CTGCTCCTTC AGGAGGATTT 
CTGGATTTAG ACAAATTCAA GAAACCTGAA GGAAGCTGGG ATTGTGAGGT CTGCTTGGTC 
CAAAACAAAG CAGAAGCCAC AAAATGTGTA GCCTGTGAAA GTGCAAAGCC AGGCACCAAA 
GCAGAGCTCA AAGGTTTTGG TACTCCTACT GTGTCCACAA ATGCTGCAAT GCCATCATTT 
ACATTTGGTG TCCAGTCGTC CTCCTCTGAA TCTTCTCATA TGTTAGGTAG CACAGGAAAT 
TTTAAATTTG GAGAACAAGG GAGCTTCAAG TTTGGCGTTG CATCAGAACC TGCGTCCTCC 
AACACTGTGA CTGGGGGTTT TAAGTTTCCT ACTAGTTCAG GGGACTTCAA ATTTGGAGTT 
TCTTCCTCAG ACTCTAAATC AGAAGACAGT AAAAAAGAAG GTAAAAGTAA CAGTTTTACC 
TTTGGACTTC CATCTACAAG CAGTCAGGCT CCTTCAACAT TTCAGTTTGG GACAGCGAGC 
CTGGGACAGC AGGAAAAGAA AGAGGAACCA GTTTTGGGAG GCTTTGGTTT TGGCACGAGT 
TCTACTTCTT CCATAGCTAC CAGTGAGAAT AAAACCGGAG TCAGTGGCTT CACTTTTGGA 
ACTGTGGCAG AAAAGGAGGT AGCATCACCT GCTCTTACAT TTAAGAAGTC AGATGAGAAA 
AAGGATGAAA CTCTTTCAAC GAAGGGAGGC TTCTCTTTTG GCAACGTGGA GTCTGCACCT 
GCCTCACAGT TTGTTTTGGG AAGGACAGAA GAGAAACAGG ACTTGGTCAC TTCTGCTGCT 
CCGTTAGCAT TTGGAAAGAA AGCTGACAGT GAAGAGTTAA AGGCACAACC TGTCTTTTCA 
GCTGGAACTG CTGAGCATAC CAAAGAGGAG AGCGCAGCAA AACCTATATT CAACTTCAGT 
TTCGTCAAAC CATCGGAGAA GGAAACCGAT CAGGCAAAGC CGTCTTTTGC GTTTGGGGCA 
CAAACCAGTA CTTCAGATCA AGGAGCAGCA AAGCCATCTT TCAGTTTCTT GAGCTCAAGT 
TCCTCCAGCA CAGTCGTACC CACCACTTCA GCCAACAGCA GCAGCGTGTT TGGCAGTGCC 
ATCTCTTCCT CAAATCCTCA GCCAGTTCCC GCTCCCTTCG TGTTTGGACA ACCCAGCAAC 
ACTGTGAGCA GCTCTGTTTT TGGCAATTCT GCAGAATCTA CAGCATCTCA GTCATTTGGC 
TTCTCTCAAG AAAACAAACC AGCAACCACA TCTTCCAGCA CCGGCACGGC TCTTGCTTCA 
TTTCTCTTTG GTTCGGGAGC CAGCAGTACC AATGCAGCAA GTTCAGGCTT TACCTTTGGA 
GCCACAACCA CATCAAGTTC AACAGGGTCA TCTTCCTCCT TTCTGTTTGG CTCCGGGCCC 
CCGGCGCCTG CAGCCGGCCC CGCGTTTGGC GCCAGCCAGC CACCAGCATT TGGCCAGAGC 
CAAGGCTCCG GCCAGCCCAG CGCTCCCAGT TTCGGATCGC TGTCGACGAC GCTGTTCTCA 
GCTGGCGCCC AGTCTGCTCC TCCCGCCTTC GGCTCGGTGA CCAGCAGCAC CCAGCCCTCC 
GTCTTCGGAC AGCAAGCCAC CCAACAGCCA GGGTTTGGCT CTGCTACCCC CAGCACTGGT 
TCCGTATTCC AGTTTGGAAG TAGTACTTCT AATTTCAACT TTCCGAGTAA CCCCGGCGTA 
TTTACTTTTG GTGCAAATAC TCCCGCACCG GCAGCACCGG CACAGCCTTC TGGCTCGTCA 
GGATTTTCCT TCAACCAGCC TCTGGCATTC ACAGTGGGGA CTAATGGAAA AAATGTTTTC 
TCTGCCTCCG GATCTTCAGT TTCTGGTCGG AAGATAAAAA CTGCTGTTAG ACGTAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796375.1
CDS	1..4383
Translation	MASGGGGGGGGGGVGGGGKIRTRRYHLGAAKPPYVRSKQQGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPSLGNTSVFKTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQMESQHPPVQKLVTPKAISLSVSRTQYFKPSLTPATDSSKIQQRVDIKHKGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSQIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940889.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940889.1

ORF Insert Sequence:

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GATGGAATCG 
CAGCATCCAC CTGTCCAGAA ACTCGTGACA CCAAAGGCAA TCTCTCTGTC AGTGAGTCGG 
ACCCAGTATT TTAAACCATC TCTGACTCCA GCTACTGATT CCAGCAAAAT TCAACAGAGG 
GTAGATATAA AGCACAAAGG CATGAGAGAG AAGAGTTTTC CTGCAGAACA GCAAGTAAAA 
CCATCTGAAA GCAATTTGAC CTACCCTAAA TTCAGTAGTC CTGCATCCAA TGGTCTGTCT 
TCAGGAGGAG TAGGTGGTGG TGGCAAGATG AGAAGGGAAA GAGGAGTACA CTATATATCA 
AAACCTGGAC AAGAACAAGA GGAGGTGGAA GAACCGGTAC TACCGAAAGT ACCCCTACCC 
ATCAGTACTG CATCGCTGCC TTCGTTCAAC TTTAGTTTTC TTCCCAGTAG TACTGTCTCA 
TCCTCACCAA GCACTGTTTC AACAGCAGTG ATGAACAAGG TACAACCAGC AAGCAATGTT 
GGCAGTCCTG TCTTCAGATT TTCATCTCCA ATTGTGAAAT CTACTGAGGC GGAAGTGCTA 
CCTCCGTTGT CTCAGATTGG GTTTACATTT AGTGTTCCTG TTGTAAAATC AGCAGAGCTT 
TCTGGATCCA GCGATACACC AGTGACGTCC TTACTTACTC TAGATACCAC CACTGTAAAC 
AGCACCAGCA ATAAAAAGGA AGAAAAAGAA GAATATGATG GCCTCACCAA GCCTGCAAAA 
GTCTTAAAGG AAGGAAGTGT GTTAGACATT CTAAAAAGCC CTGGCTTCAC GTCTGAGAAA 
ACACACTCCT CTACATCCGC ACAGCCTGTT ACGAGCACAG TGGTCTATAC AAGGCCTGCA 
ATAAGTAGTT TTTCTGCAGG CAAAGACAGC CCTAAACAGC CATCCTCGTT TTGGCAGTCT 
GACACGCATG ACCCATGTCT GCAAAACAAA CCCACAGATG GCAAATGTGT AACGTGTCAA 
GCTGCTAAAG TGTCAACAGT GGAGAGTACG AAACCCACGA TAAGCTCTAG TCCGTGCGTC 
ACCTCGAAGG CCGCAGTCCC TACAGCCGGG ACACTGGGCT TTGAAGACAA ATTTAAACCA 
CCACCCAACA CATGGGATTG TGATACCTGT CTGGTCCAGA ACAAACCTGA AGCTACAAAA 
TGTATAGCCT GTGAGACACC AAAGCCTGGG ACAGGAGTAA TGCCTGCTCT GACATTGCCA 
GTGGTCACAG ACAGCTCGGT GACAGTGACA TCCTCCTCCA GCAGCACTGA CACAACGGCC 
ACTTTGGGAT TTGGAGACAA ATTTAAGAAG CCAAAAGGCT CTTGGGAGTG TGCAGTGTGC 
CTTGTATCAA ATAAGGCAGA AGACAGCAAA TGTGTAGCCT GTCAGTCTGA GAAACCAGGG 
AGTTCAGTGC CTGTGACCAG TAGCAGTGTT TCTACATTTT CTGCTCCTTC AGGAGGATTT 
CTGGATTTAG ACAAATTCAA GAAACCTGAA GGAAGCTGGG ATTGTGAGGT CTGCTTGGTC 
CAAAACAAAG CAGAAGCCAC AAAATGTGTA GCCTGTGAAA GTGCAAAGCC AGGCACCAAA 
GCAGAGCTCA AAGGTTTTGG TACTCCTACT GTGTCCACAA ATGCTGCAAT GCCATCATTT 
ACATTTGGTG TCCAGTCGTC CTCCTCTGAA TCTTCTCATA TGTTAGGTAG CACAGGAAAT 
TTTAAATTTG GAGAACAAGG GAGCTTCAAG TTTGGCGTTG CATCAGAACC TGCGTCCTCC 
AACACTGTGA CTGGGGGTTT TAAGTTTCCT ACTAGTTCAG GGGACTTCAA ATTTGGAGTT 
TCTTCCTCAG ACTCTAAATC AGAAGACAGT AAAAAAGAAG GTAAAAGTAA CAGTTTTACC 
TTTGGACTTC CATCTACAAG CAGTCAGGCT CCTTCAACAT TTCAGTTTGG GACAGCGAGC 
CTGGGACAGC AGGAAAAGAA AGAGGAACCA GTTTTGGGAG GCTTTGGTTT TGGCACGAGT 
TCTACTTCTT CCATAGCTAC CAGTGAGAAT AAAACCGGAG TCAGTGGCTT CACTTTTGGA 
ACTGTGGCAG AAAAGGAGGT AGCATCACCT GCTCTTACAT TTAAGAAGTC AGATGAGAAA 
AAGGATGAAA CTCTTTCAAC GAAGGGAGGC TTCTCTTTTG GCAACGTGGA GTCTGCACCT 
GCCTCACAGT TTGTTTTGGG AAGGACAGAA GAGAAACAGG ACTTGGTCAC TTCTGCTGCT 
CCGTTAGCAT TTGGAAAGAA AGCTGACAGT GAAGAGTTAA AGGCACAACC TGTCTTTTCA 
GCTGGAACTG CTGAGCATAC CAAAGAGGAG AGCGCAGCAA AACCTATATT CAACTTCAGT 
TTCGTCAAAC CATCGGAGAA GGAAACCGAT CAGGCAAAGC CGTCTTTTGC GTTTGGGGCA 
CAAACCAGTA CTTCAGATCA AGGAGCAGCA AAGCCATCTT TCAGTTTCTT GAGCTCAAGT 
TCCTCCAGCA CAGTCGTACC CACCACTTCA GCCAACAGCA GCAGCGTGTT TGGCAGTGCC 
ATCTCTTCCT CAAATCCTCA GCCAGTTCCC GCTCCCTTCG TGTTTGGACA ACCCAGCAAC 
ACTGTGAGCA GCTCTGTTTT TGGCAATTCT GCAGAATCTA CAGCATCTCA GTCATTTGGC 
TTCTCTCAAG AAAACAAACC AGCAACCACA TCTTCCAGCA CCGGCACGGC TCTTGCTTCA 
TTTCTCTTTG GTTCGGGAGC CAGCAGTACC AATGCAGCAA GTTCAGGCTT TACCTTTGGA 
GCCACAACCA CATCAAGTTC AACAGGGTCA TCTTCCTCCT TTCTGTTTGG CTCCGGGCCC 
CCGGCGCCTG CAGCCGGCCC CGCGTTTGGC GCCAGCCAGC CACCAGCATT TGGCCAGAGC 
CAAGGCTCCG GCCAGCCCAG CGCTCCCAGT TTCGGATCGC TGTCGACGAC GCTGTTCTCA 
GCTGGCGCCC AGTCTGCTCC TCCCGCCTTC GGCTCGGTGA CCAGCAGCAC CCAGCCCTCC 
GTCTTCGGAC AGCAAGCCAC CCAACAGCCA GGGTTTGGCT CTGCTACCCC CAGCACTGGT 
TCCGTATTCC AGTTTGGAAG TAGTACTTCT AATTTCAACT TTCCGAGTAA CCCCGGCGTA 
TTTACTTTTG GTGCAAATAC TCCCGCACCG GCAGCACCGG CACAGCCTTC TGGCTCGTCA 
GGATTTTCCT TCAACCAGCC TCTGGCATTC ACAGTGGGGA CTAATGGAAA AAATGTTTTC 
TCTGCCTCCG GATCTTCAGT TTCTGGTCGG AAGATAAAAA CTGCTGTTAG ACGTAGAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP153 ( XM_014940890.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796376.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X2, mRNA.

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CloneID	OCa64251
Clone ID Related Accession (Same CDS sequence)	XM_014940890.1
Accession Version	XM_014940890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGGGA 
ATCATTAGCA GAGTTACAGA ATCGGTGAAA AATATTGTAC CGGGATGGTT ACAGAAGTAT 
TTCAATAGAA GTGAAGATGA ATGTGTAGAT ACAAATGAAT CTACAAACCA GGAAGAAAGT 
CCAGTAAACT ATCACCATGA TTATGCAAAT GAAGATACCA TAGTGATTGA TGGGAGAGTC 
ACGCCTGAAT CAGCAAGAAT TAATTTAGAA GAACCATCCA CGAGTAGATC TGCATTGAAC 
TTCTCAGATG TGTTAACAAG GCCCTCTCTT CATCGGAGTC ATTTGAATTG TACCATGTTG 
GATTCCACAG TACCACATTG TCAGCCCTCT ACATCTTCTT CACTTGGCAT TGGCAGTCCT 
GGACTGTCTC TTGTAAAAGA AATAAAAGAT TCTACCTCTC AGCATGATGA CGATAACATC 
TCAACAACCA GTGGCTTCTC CTCTAGAGCA TCTGATAAAG ATATCACAGT TTCAAAAAAT 
ACTTCAGCGC CGCTGCTCTG GTCCACAGAG GCTGAAAGAT CTCATTCCCT CTCACAGCAT 
TCTGCAACTA GCTCTAAAAA ACCTGCATTC AATTTATCTG CTTTTGGATC TCCCTCTCCT 
TCACTTGGAA ACACTTCTGT CTTTAAGACA AGCCAGCTTG GGGATTCTCC ATTTTACCCT 
GGAAAGACGA CGTACGGTGG TGCAGCTGCA GCAGCAAGGG AGACAAAAGC ACGAATTGCT 
CCTTATCAGC CCCCGGTAAG AAGACAAGTG AAAGCTAAAC AAGCAAGTGT GCAATCCTAT 
GGTGTGACAA GTTCCACAGC GCGGCGCATC TTGCAGTCAT TGGAGAAGAT GTCAAGTCCT 
TTGGCGGATG CTAAAAGGAT TCCATCTTCT GTTTCTAGTC CACTGTCTTC TCCTGTGGAC 
AGGGGTGCAC AGCTGAATAT TGCTAGCTTC CAATCCAAAC GAAAACAGAT GGAATCGCAG 
CATCCACCTG TCCAGAAACT CGTGACACCA AAGGCAATCT CTCTGTCAGT GAGTCGGACC 
CAGTATTTTA AACCATCTCT GACTCCAGCT ACTGATTCCA GCAAAATTCA ACAGAGGGTA 
GATATAAAGC ACAAAGGCAT GAGAGAGAAG AGTTTTCCTG CAGAACAGCA AGTAAAACCA 
TCTGAAAGCA ATTTGACCTA CCCTAAATTC AGTAGTCCTG CATCCAATGG TCTGTCTTCA 
GGAGGAGTAG GTGGTGGTGG CAAGATGAGA AGGGAAAGAG GAGTACACTA TATATCAAAA 
CCTGGACAAG AACAAGAGGA GGTGGAAGAA CCGGTACTAC CGAAAGTACC CCTACCCATC 
AGTACTGCAT CGCTGCCTTC GTTCAACTTT AGTTTTCTTC CCAGTAGTAC TGTCTCATCC 
TCACCAAGCA CTGTTTCAAC AGCAGTGATG AACAAGGTAC AACCAGCAAG CAATGTTGGC 
AGTCCTGTCT TCAGATTTTC ATCTCCAATT GTGAAATCTA CTGAGGCGGA AGTGCTACCT 
CCGTTGTCTC AGATTGGGTT TACATTTAGT GTTCCTGTTG TAAAATCAGC AGAGCTTTCT 
GGATCCAGCG ATACACCAGT GACGTCCTTA CTTACTCTAG ATACCACCAC TGTAAACAGC 
ACCAGCAATA AAAAGGAAGA AAAAGAAGAA TATGATGGCC TCACCAAGCC TGCAAAAGTC 
TTAAAGGAAG GAAGTGTGTT AGACATTCTA AAAAGCCCTG GCTTCACGTC TGAGAAAACA 
CACTCCTCTA CATCCGCACA GCCTGTTACG AGCACAGTGG TCTATACAAG GCCTGCAATA 
AGTAGTTTTT CTGCAGGCAA AGACAGCCCT AAACAGCCAT CCTCGTTTTG GCAGTCTGAC 
ACGCATGACC CATGTCTGCA AAACAAACCC ACAGATGGCA AATGTGTAAC GTGTCAAGCT 
GCTAAAGTGT CAACAGTGGA GAGTACGAAA CCCACGATAA GCTCTAGTCC GTGCGTCACC 
TCGAAGGCCG CAGTCCCTAC AGCCGGGACA CTGGGCTTTG AAGACAAATT TAAACCACCA 
CCCAACACAT GGGATTGTGA TACCTGTCTG GTCCAGAACA AACCTGAAGC TACAAAATGT 
ATAGCCTGTG AGACACCAAA GCCTGGGACA GGAGTAATGC CTGCTCTGAC ATTGCCAGTG 
GTCACAGACA GCTCGGTGAC AGTGACATCC TCCTCCAGCA GCACTGACAC AACGGCCACT 
TTGGGATTTG GAGACAAATT TAAGAAGCCA AAAGGCTCTT GGGAGTGTGC AGTGTGCCTT 
GTATCAAATA AGGCAGAAGA CAGCAAATGT GTAGCCTGTC AGTCTGAGAA ACCAGGGAGT 
TCAGTGCCTG TGACCAGTAG CAGTGTTTCT ACATTTTCTG CTCCTTCAGG AGGATTTCTG 
GATTTAGACA AATTCAAGAA ACCTGAAGGA AGCTGGGATT GTGAGGTCTG CTTGGTCCAA 
AACAAAGCAG AAGCCACAAA ATGTGTAGCC TGTGAAAGTG CAAAGCCAGG CACCAAAGCA 
GAGCTCAAAG GTTTTGGTAC TCCTACTGTG TCCACAAATG CTGCAATGCC ATCATTTACA 
TTTGGTGTCC AGTCGTCCTC CTCTGAATCT TCTCATATGT TAGGTAGCAC AGGAAATTTT 
AAATTTGGAG AACAAGGGAG CTTCAAGTTT GGCGTTGCAT CAGAACCTGC GTCCTCCAAC 
ACTGTGACTG GGGGTTTTAA GTTTCCTACT AGTTCAGGGG ACTTCAAATT TGGAGTTTCT 
TCCTCAGACT CTAAATCAGA AGACAGTAAA AAAGAAGGTA AAAGTAACAG TTTTACCTTT 
GGACTTCCAT CTACAAGCAG TCAGGCTCCT TCAACATTTC AGTTTGGGAC AGCGAGCCTG 
GGACAGCAGG AAAAGAAAGA GGAACCAGTT TTGGGAGGCT TTGGTTTTGG CACGAGTTCT 
ACTTCTTCCA TAGCTACCAG TGAGAATAAA ACCGGAGTCA GTGGCTTCAC TTTTGGAACT 
GTGGCAGAAA AGGAGGTAGC ATCACCTGCT CTTACATTTA AGAAGTCAGA TGAGAAAAAG 
GATGAAACTC TTTCAACGAA GGGAGGCTTC TCTTTTGGCA ACGTGGAGTC TGCACCTGCC 
TCACAGTTTG TTTTGGGAAG GACAGAAGAG AAACAGGACT TGGTCACTTC TGCTGCTCCG 
TTAGCATTTG GAAAGAAAGC TGACAGTGAA GAGTTAAAGG CACAACCTGT CTTTTCAGCT 
GGAACTGCTG AGCATACCAA AGAGGAGAGC GCAGCAAAAC CTATATTCAA CTTCAGTTTC 
GTCAAACCAT CGGAGAAGGA AACCGATCAG GCAAAGCCGT CTTTTGCGTT TGGGGCACAA 
ACCAGTACTT CAGATCAAGG AGCAGCAAAG CCATCTTTCA GTTTCTTGAG CTCAAGTTCC 
TCCAGCACAG TCGTACCCAC CACTTCAGCC AACAGCAGCA GCGTGTTTGG CAGTGCCATC 
TCTTCCTCAA ATCCTCAGCC AGTTCCCGCT CCCTTCGTGT TTGGACAACC CAGCAACACT 
GTGAGCAGCT CTGTTTTTGG CAATTCTGCA GAATCTACAG CATCTCAGTC ATTTGGCTTC 
TCTCAAGAAA ACAAACCAGC AACCACATCT TCCAGCACCG GCACGGCTCT TGCTTCATTT 
CTCTTTGGTT CGGGAGCCAG CAGTACCAAT GCAGCAAGTT CAGGCTTTAC CTTTGGAGCC 
ACAACCACAT CAAGTTCAAC AGGGTCATCT TCCTCCTTTC TGTTTGGCTC CGGGCCCCCG 
GCGCCTGCAG CCGGCCCCGC GTTTGGCGCC AGCCAGCCAC CAGCATTTGG CCAGAGCCAA 
GGCTCCGGCC AGCCCAGCGC TCCCAGTTTC GGATCGCTGT CGACGACGCT GTTCTCAGCT 
GGCGCCCAGT CTGCTCCTCC CGCCTTCGGC TCGGTGACCA GCAGCACCCA GCCCTCCGTC 
TTCGGACAGC AAGCCACCCA ACAGCCAGGG TTTGGCTCTG CTACCCCCAG CACTGGTTCC 
GTATTCCAGT TTGGAAGTAG TACTTCTAAT TTCAACTTTC CGAGTAACCC CGGCGTATTT 
ACTTTTGGTG CAAATACTCC CGCACCGGCA GCACCGGCAC AGCCTTCTGG CTCGTCAGGA 
TTTTCCTTCA ACCAGCCTCT GGCATTCACA GTGGGGACTA ATGGAAAAAA TGTTTTCTCT 
GCCTCCGGAT CTTCAGTTTC TGGTCGGAAG ATAAAAACTG CTGTTAGACG TAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796376.1
CDS	1..4380
Translation	MASGGGGGGGGGGVGGGGKIRTRRYHLGAAKPPYVRSKQGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPSLGNTSVFKTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQMESQHPPVQKLVTPKAISLSVSRTQYFKPSLTPATDSSKIQQRVDIKHKGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSQIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940890.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940890.1

ORF Insert Sequence:

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGGGA 
ATCATTAGCA GAGTTACAGA ATCGGTGAAA AATATTGTAC CGGGATGGTT ACAGAAGTAT 
TTCAATAGAA GTGAAGATGA ATGTGTAGAT ACAAATGAAT CTACAAACCA GGAAGAAAGT 
CCAGTAAACT ATCACCATGA TTATGCAAAT GAAGATACCA TAGTGATTGA TGGGAGAGTC 
ACGCCTGAAT CAGCAAGAAT TAATTTAGAA GAACCATCCA CGAGTAGATC TGCATTGAAC 
TTCTCAGATG TGTTAACAAG GCCCTCTCTT CATCGGAGTC ATTTGAATTG TACCATGTTG 
GATTCCACAG TACCACATTG TCAGCCCTCT ACATCTTCTT CACTTGGCAT TGGCAGTCCT 
GGACTGTCTC TTGTAAAAGA AATAAAAGAT TCTACCTCTC AGCATGATGA CGATAACATC 
TCAACAACCA GTGGCTTCTC CTCTAGAGCA TCTGATAAAG ATATCACAGT TTCAAAAAAT 
ACTTCAGCGC CGCTGCTCTG GTCCACAGAG GCTGAAAGAT CTCATTCCCT CTCACAGCAT 
TCTGCAACTA GCTCTAAAAA ACCTGCATTC AATTTATCTG CTTTTGGATC TCCCTCTCCT 
TCACTTGGAA ACACTTCTGT CTTTAAGACA AGCCAGCTTG GGGATTCTCC ATTTTACCCT 
GGAAAGACGA CGTACGGTGG TGCAGCTGCA GCAGCAAGGG AGACAAAAGC ACGAATTGCT 
CCTTATCAGC CCCCGGTAAG AAGACAAGTG AAAGCTAAAC AAGCAAGTGT GCAATCCTAT 
GGTGTGACAA GTTCCACAGC GCGGCGCATC TTGCAGTCAT TGGAGAAGAT GTCAAGTCCT 
TTGGCGGATG CTAAAAGGAT TCCATCTTCT GTTTCTAGTC CACTGTCTTC TCCTGTGGAC 
AGGGGTGCAC AGCTGAATAT TGCTAGCTTC CAATCCAAAC GAAAACAGAT GGAATCGCAG 
CATCCACCTG TCCAGAAACT CGTGACACCA AAGGCAATCT CTCTGTCAGT GAGTCGGACC 
CAGTATTTTA AACCATCTCT GACTCCAGCT ACTGATTCCA GCAAAATTCA ACAGAGGGTA 
GATATAAAGC ACAAAGGCAT GAGAGAGAAG AGTTTTCCTG CAGAACAGCA AGTAAAACCA 
TCTGAAAGCA ATTTGACCTA CCCTAAATTC AGTAGTCCTG CATCCAATGG TCTGTCTTCA 
GGAGGAGTAG GTGGTGGTGG CAAGATGAGA AGGGAAAGAG GAGTACACTA TATATCAAAA 
CCTGGACAAG AACAAGAGGA GGTGGAAGAA CCGGTACTAC CGAAAGTACC CCTACCCATC 
AGTACTGCAT CGCTGCCTTC GTTCAACTTT AGTTTTCTTC CCAGTAGTAC TGTCTCATCC 
TCACCAAGCA CTGTTTCAAC AGCAGTGATG AACAAGGTAC AACCAGCAAG CAATGTTGGC 
AGTCCTGTCT TCAGATTTTC ATCTCCAATT GTGAAATCTA CTGAGGCGGA AGTGCTACCT 
CCGTTGTCTC AGATTGGGTT TACATTTAGT GTTCCTGTTG TAAAATCAGC AGAGCTTTCT 
GGATCCAGCG ATACACCAGT GACGTCCTTA CTTACTCTAG ATACCACCAC TGTAAACAGC 
ACCAGCAATA AAAAGGAAGA AAAAGAAGAA TATGATGGCC TCACCAAGCC TGCAAAAGTC 
TTAAAGGAAG GAAGTGTGTT AGACATTCTA AAAAGCCCTG GCTTCACGTC TGAGAAAACA 
CACTCCTCTA CATCCGCACA GCCTGTTACG AGCACAGTGG TCTATACAAG GCCTGCAATA 
AGTAGTTTTT CTGCAGGCAA AGACAGCCCT AAACAGCCAT CCTCGTTTTG GCAGTCTGAC 
ACGCATGACC CATGTCTGCA AAACAAACCC ACAGATGGCA AATGTGTAAC GTGTCAAGCT 
GCTAAAGTGT CAACAGTGGA GAGTACGAAA CCCACGATAA GCTCTAGTCC GTGCGTCACC 
TCGAAGGCCG CAGTCCCTAC AGCCGGGACA CTGGGCTTTG AAGACAAATT TAAACCACCA 
CCCAACACAT GGGATTGTGA TACCTGTCTG GTCCAGAACA AACCTGAAGC TACAAAATGT 
ATAGCCTGTG AGACACCAAA GCCTGGGACA GGAGTAATGC CTGCTCTGAC ATTGCCAGTG 
GTCACAGACA GCTCGGTGAC AGTGACATCC TCCTCCAGCA GCACTGACAC AACGGCCACT 
TTGGGATTTG GAGACAAATT TAAGAAGCCA AAAGGCTCTT GGGAGTGTGC AGTGTGCCTT 
GTATCAAATA AGGCAGAAGA CAGCAAATGT GTAGCCTGTC AGTCTGAGAA ACCAGGGAGT 
TCAGTGCCTG TGACCAGTAG CAGTGTTTCT ACATTTTCTG CTCCTTCAGG AGGATTTCTG 
GATTTAGACA AATTCAAGAA ACCTGAAGGA AGCTGGGATT GTGAGGTCTG CTTGGTCCAA 
AACAAAGCAG AAGCCACAAA ATGTGTAGCC TGTGAAAGTG CAAAGCCAGG CACCAAAGCA 
GAGCTCAAAG GTTTTGGTAC TCCTACTGTG TCCACAAATG CTGCAATGCC ATCATTTACA 
TTTGGTGTCC AGTCGTCCTC CTCTGAATCT TCTCATATGT TAGGTAGCAC AGGAAATTTT 
AAATTTGGAG AACAAGGGAG CTTCAAGTTT GGCGTTGCAT CAGAACCTGC GTCCTCCAAC 
ACTGTGACTG GGGGTTTTAA GTTTCCTACT AGTTCAGGGG ACTTCAAATT TGGAGTTTCT 
TCCTCAGACT CTAAATCAGA AGACAGTAAA AAAGAAGGTA AAAGTAACAG TTTTACCTTT 
GGACTTCCAT CTACAAGCAG TCAGGCTCCT TCAACATTTC AGTTTGGGAC AGCGAGCCTG 
GGACAGCAGG AAAAGAAAGA GGAACCAGTT TTGGGAGGCT TTGGTTTTGG CACGAGTTCT 
ACTTCTTCCA TAGCTACCAG TGAGAATAAA ACCGGAGTCA GTGGCTTCAC TTTTGGAACT 
GTGGCAGAAA AGGAGGTAGC ATCACCTGCT CTTACATTTA AGAAGTCAGA TGAGAAAAAG 
GATGAAACTC TTTCAACGAA GGGAGGCTTC TCTTTTGGCA ACGTGGAGTC TGCACCTGCC 
TCACAGTTTG TTTTGGGAAG GACAGAAGAG AAACAGGACT TGGTCACTTC TGCTGCTCCG 
TTAGCATTTG GAAAGAAAGC TGACAGTGAA GAGTTAAAGG CACAACCTGT CTTTTCAGCT 
GGAACTGCTG AGCATACCAA AGAGGAGAGC GCAGCAAAAC CTATATTCAA CTTCAGTTTC 
GTCAAACCAT CGGAGAAGGA AACCGATCAG GCAAAGCCGT CTTTTGCGTT TGGGGCACAA 
ACCAGTACTT CAGATCAAGG AGCAGCAAAG CCATCTTTCA GTTTCTTGAG CTCAAGTTCC 
TCCAGCACAG TCGTACCCAC CACTTCAGCC AACAGCAGCA GCGTGTTTGG CAGTGCCATC 
TCTTCCTCAA ATCCTCAGCC AGTTCCCGCT CCCTTCGTGT TTGGACAACC CAGCAACACT 
GTGAGCAGCT CTGTTTTTGG CAATTCTGCA GAATCTACAG CATCTCAGTC ATTTGGCTTC 
TCTCAAGAAA ACAAACCAGC AACCACATCT TCCAGCACCG GCACGGCTCT TGCTTCATTT 
CTCTTTGGTT CGGGAGCCAG CAGTACCAAT GCAGCAAGTT CAGGCTTTAC CTTTGGAGCC 
ACAACCACAT CAAGTTCAAC AGGGTCATCT TCCTCCTTTC TGTTTGGCTC CGGGCCCCCG 
GCGCCTGCAG CCGGCCCCGC GTTTGGCGCC AGCCAGCCAC CAGCATTTGG CCAGAGCCAA 
GGCTCCGGCC AGCCCAGCGC TCCCAGTTTC GGATCGCTGT CGACGACGCT GTTCTCAGCT 
GGCGCCCAGT CTGCTCCTCC CGCCTTCGGC TCGGTGACCA GCAGCACCCA GCCCTCCGTC 
TTCGGACAGC AAGCCACCCA ACAGCCAGGG TTTGGCTCTG CTACCCCCAG CACTGGTTCC 
GTATTCCAGT TTGGAAGTAG TACTTCTAAT TTCAACTTTC CGAGTAACCC CGGCGTATTT 
ACTTTTGGTG CAAATACTCC CGCACCGGCA GCACCGGCAC AGCCTTCTGG CTCGTCAGGA 
TTTTCCTTCA ACCAGCCTCT GGCATTCACA GTGGGGACTA ATGGAAAAAA TGTTTTCTCT 
GCCTCCGGAT CTTCAGTTTC TGGTCGGAAG ATAAAAACTG CTGTTAGACG TAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP153 ( XM_014940891.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796377.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X3, mRNA.

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CloneID	OCa64252
Clone ID Related Accession (Same CDS sequence)	XM_014940891.1
Accession Version	XM_014940891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GATGGAATCG 
CAGCATCCAC CTGTCCAGAA ACTCGTGACA CCAAAGGCAA TCTCTCTGTC AGTGAGTCGG 
ACCCAGTATT TTAAACCATC TCTGACTCCA GCTACTGATT CCAGCAAAAT TCAACAGAGG 
GTAGATATAA AGCACAAAGG CATGAGAGAG AAGAGTTTTC CTGCAGAACA GCAAGTAAAA 
CCATCTGAAA GCAATTTGAC CTACCCTAAA TTCAGTAGTC CTGCATCCAA TGGTCTGTCT 
TCAGGAGGAG TAGGTGGTGG TGGCAAGATG AGAAGGGAAA GAGGAGTACA CTATATATCA 
AAACCTGGAC AAGAACAAGA GGAGGTGGAA GAACCGGTAC TACCGAAAGT ACCCCTACCC 
ATCAGTACTG CATCGCTGCC TTCGTTCAAC TTTAGTTTTC TTCCCAGTAG TACTGTCTCA 
TCCTCACCAA GCACTGTTTC AACAGCAGTG ATGAACAAGG TACAACCAGC AAGCAATGTT 
GGCAGTCCTG TCTTCAGATT TTCATCTCCA ATTGTGAAAT CTACTGAGGC GGAAGTGCTA 
CCTCCGTTGT CTATTGGGTT TACATTTAGT GTTCCTGTTG TAAAATCAGC AGAGCTTTCT 
GGATCCAGCG ATACACCAGT GACGTCCTTA CTTACTCTAG ATACCACCAC TGTAAACAGC 
ACCAGCAATA AAAAGGAAGA AAAAGAAGAA TATGATGGCC TCACCAAGCC TGCAAAAGTC 
TTAAAGGAAG GAAGTGTGTT AGACATTCTA AAAAGCCCTG GCTTCACGTC TGAGAAAACA 
CACTCCTCTA CATCCGCACA GCCTGTTACG AGCACAGTGG TCTATACAAG GCCTGCAATA 
AGTAGTTTTT CTGCAGGCAA AGACAGCCCT AAACAGCCAT CCTCGTTTTG GCAGTCTGAC 
ACGCATGACC CATGTCTGCA AAACAAACCC ACAGATGGCA AATGTGTAAC GTGTCAAGCT 
GCTAAAGTGT CAACAGTGGA GAGTACGAAA CCCACGATAA GCTCTAGTCC GTGCGTCACC 
TCGAAGGCCG CAGTCCCTAC AGCCGGGACA CTGGGCTTTG AAGACAAATT TAAACCACCA 
CCCAACACAT GGGATTGTGA TACCTGTCTG GTCCAGAACA AACCTGAAGC TACAAAATGT 
ATAGCCTGTG AGACACCAAA GCCTGGGACA GGAGTAATGC CTGCTCTGAC ATTGCCAGTG 
GTCACAGACA GCTCGGTGAC AGTGACATCC TCCTCCAGCA GCACTGACAC AACGGCCACT 
TTGGGATTTG GAGACAAATT TAAGAAGCCA AAAGGCTCTT GGGAGTGTGC AGTGTGCCTT 
GTATCAAATA AGGCAGAAGA CAGCAAATGT GTAGCCTGTC AGTCTGAGAA ACCAGGGAGT 
TCAGTGCCTG TGACCAGTAG CAGTGTTTCT ACATTTTCTG CTCCTTCAGG AGGATTTCTG 
GATTTAGACA AATTCAAGAA ACCTGAAGGA AGCTGGGATT GTGAGGTCTG CTTGGTCCAA 
AACAAAGCAG AAGCCACAAA ATGTGTAGCC TGTGAAAGTG CAAAGCCAGG CACCAAAGCA 
GAGCTCAAAG GTTTTGGTAC TCCTACTGTG TCCACAAATG CTGCAATGCC ATCATTTACA 
TTTGGTGTCC AGTCGTCCTC CTCTGAATCT TCTCATATGT TAGGTAGCAC AGGAAATTTT 
AAATTTGGAG AACAAGGGAG CTTCAAGTTT GGCGTTGCAT CAGAACCTGC GTCCTCCAAC 
ACTGTGACTG GGGGTTTTAA GTTTCCTACT AGTTCAGGGG ACTTCAAATT TGGAGTTTCT 
TCCTCAGACT CTAAATCAGA AGACAGTAAA AAAGAAGGTA AAAGTAACAG TTTTACCTTT 
GGACTTCCAT CTACAAGCAG TCAGGCTCCT TCAACATTTC AGTTTGGGAC AGCGAGCCTG 
GGACAGCAGG AAAAGAAAGA GGAACCAGTT TTGGGAGGCT TTGGTTTTGG CACGAGTTCT 
ACTTCTTCCA TAGCTACCAG TGAGAATAAA ACCGGAGTCA GTGGCTTCAC TTTTGGAACT 
GTGGCAGAAA AGGAGGTAGC ATCACCTGCT CTTACATTTA AGAAGTCAGA TGAGAAAAAG 
GATGAAACTC TTTCAACGAA GGGAGGCTTC TCTTTTGGCA ACGTGGAGTC TGCACCTGCC 
TCACAGTTTG TTTTGGGAAG GACAGAAGAG AAACAGGACT TGGTCACTTC TGCTGCTCCG 
TTAGCATTTG GAAAGAAAGC TGACAGTGAA GAGTTAAAGG CACAACCTGT CTTTTCAGCT 
GGAACTGCTG AGCATACCAA AGAGGAGAGC GCAGCAAAAC CTATATTCAA CTTCAGTTTC 
GTCAAACCAT CGGAGAAGGA AACCGATCAG GCAAAGCCGT CTTTTGCGTT TGGGGCACAA 
ACCAGTACTT CAGATCAAGG AGCAGCAAAG CCATCTTTCA GTTTCTTGAG CTCAAGTTCC 
TCCAGCACAG TCGTACCCAC CACTTCAGCC AACAGCAGCA GCGTGTTTGG CAGTGCCATC 
TCTTCCTCAA ATCCTCAGCC AGTTCCCGCT CCCTTCGTGT TTGGACAACC CAGCAACACT 
GTGAGCAGCT CTGTTTTTGG CAATTCTGCA GAATCTACAG CATCTCAGTC ATTTGGCTTC 
TCTCAAGAAA ACAAACCAGC AACCACATCT TCCAGCACCG GCACGGCTCT TGCTTCATTT 
CTCTTTGGTT CGGGAGCCAG CAGTACCAAT GCAGCAAGTT CAGGCTTTAC CTTTGGAGCC 
ACAACCACAT CAAGTTCAAC AGGGTCATCT TCCTCCTTTC TGTTTGGCTC CGGGCCCCCG 
GCGCCTGCAG CCGGCCCCGC GTTTGGCGCC AGCCAGCCAC CAGCATTTGG CCAGAGCCAA 
GGCTCCGGCC AGCCCAGCGC TCCCAGTTTC GGATCGCTGT CGACGACGCT GTTCTCAGCT 
GGCGCCCAGT CTGCTCCTCC CGCCTTCGGC TCGGTGACCA GCAGCACCCA GCCCTCCGTC 
TTCGGACAGC AAGCCACCCA ACAGCCAGGG TTTGGCTCTG CTACCCCCAG CACTGGTTCC 
GTATTCCAGT TTGGAAGTAG TACTTCTAAT TTCAACTTTC CGAGTAACCC CGGCGTATTT 
ACTTTTGGTG CAAATACTCC CGCACCGGCA GCACCGGCAC AGCCTTCTGG CTCGTCAGGA 
TTTTCCTTCA ACCAGCCTCT GGCATTCACA GTGGGGACTA ATGGAAAAAA TGTTTTCTCT 
GCCTCCGGAT CTTCAGTTTC TGGTCGGAAG ATAAAAACTG CTGTTAGACG TAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796377.1
CDS	1..4380
Translation	MASGGGGGGGGGGVGGGGKIRTRRYHLGAAKPPYVRSKQQGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPSLGNTSVFKTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQMESQHPPVQKLVTPKAISLSVSRTQYFKPSLTPATDSSKIQQRVDIKHKGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940891.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940891.1

ORF Insert Sequence:

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GATGGAATCG 
CAGCATCCAC CTGTCCAGAA ACTCGTGACA CCAAAGGCAA TCTCTCTGTC AGTGAGTCGG 
ACCCAGTATT TTAAACCATC TCTGACTCCA GCTACTGATT CCAGCAAAAT TCAACAGAGG 
GTAGATATAA AGCACAAAGG CATGAGAGAG AAGAGTTTTC CTGCAGAACA GCAAGTAAAA 
CCATCTGAAA GCAATTTGAC CTACCCTAAA TTCAGTAGTC CTGCATCCAA TGGTCTGTCT 
TCAGGAGGAG TAGGTGGTGG TGGCAAGATG AGAAGGGAAA GAGGAGTACA CTATATATCA 
AAACCTGGAC AAGAACAAGA GGAGGTGGAA GAACCGGTAC TACCGAAAGT ACCCCTACCC 
ATCAGTACTG CATCGCTGCC TTCGTTCAAC TTTAGTTTTC TTCCCAGTAG TACTGTCTCA 
TCCTCACCAA GCACTGTTTC AACAGCAGTG ATGAACAAGG TACAACCAGC AAGCAATGTT 
GGCAGTCCTG TCTTCAGATT TTCATCTCCA ATTGTGAAAT CTACTGAGGC GGAAGTGCTA 
CCTCCGTTGT CTATTGGGTT TACATTTAGT GTTCCTGTTG TAAAATCAGC AGAGCTTTCT 
GGATCCAGCG ATACACCAGT GACGTCCTTA CTTACTCTAG ATACCACCAC TGTAAACAGC 
ACCAGCAATA AAAAGGAAGA AAAAGAAGAA TATGATGGCC TCACCAAGCC TGCAAAAGTC 
TTAAAGGAAG GAAGTGTGTT AGACATTCTA AAAAGCCCTG GCTTCACGTC TGAGAAAACA 
CACTCCTCTA CATCCGCACA GCCTGTTACG AGCACAGTGG TCTATACAAG GCCTGCAATA 
AGTAGTTTTT CTGCAGGCAA AGACAGCCCT AAACAGCCAT CCTCGTTTTG GCAGTCTGAC 
ACGCATGACC CATGTCTGCA AAACAAACCC ACAGATGGCA AATGTGTAAC GTGTCAAGCT 
GCTAAAGTGT CAACAGTGGA GAGTACGAAA CCCACGATAA GCTCTAGTCC GTGCGTCACC 
TCGAAGGCCG CAGTCCCTAC AGCCGGGACA CTGGGCTTTG AAGACAAATT TAAACCACCA 
CCCAACACAT GGGATTGTGA TACCTGTCTG GTCCAGAACA AACCTGAAGC TACAAAATGT 
ATAGCCTGTG AGACACCAAA GCCTGGGACA GGAGTAATGC CTGCTCTGAC ATTGCCAGTG 
GTCACAGACA GCTCGGTGAC AGTGACATCC TCCTCCAGCA GCACTGACAC AACGGCCACT 
TTGGGATTTG GAGACAAATT TAAGAAGCCA AAAGGCTCTT GGGAGTGTGC AGTGTGCCTT 
GTATCAAATA AGGCAGAAGA CAGCAAATGT GTAGCCTGTC AGTCTGAGAA ACCAGGGAGT 
TCAGTGCCTG TGACCAGTAG CAGTGTTTCT ACATTTTCTG CTCCTTCAGG AGGATTTCTG 
GATTTAGACA AATTCAAGAA ACCTGAAGGA AGCTGGGATT GTGAGGTCTG CTTGGTCCAA 
AACAAAGCAG AAGCCACAAA ATGTGTAGCC TGTGAAAGTG CAAAGCCAGG CACCAAAGCA 
GAGCTCAAAG GTTTTGGTAC TCCTACTGTG TCCACAAATG CTGCAATGCC ATCATTTACA 
TTTGGTGTCC AGTCGTCCTC CTCTGAATCT TCTCATATGT TAGGTAGCAC AGGAAATTTT 
AAATTTGGAG AACAAGGGAG CTTCAAGTTT GGCGTTGCAT CAGAACCTGC GTCCTCCAAC 
ACTGTGACTG GGGGTTTTAA GTTTCCTACT AGTTCAGGGG ACTTCAAATT TGGAGTTTCT 
TCCTCAGACT CTAAATCAGA AGACAGTAAA AAAGAAGGTA AAAGTAACAG TTTTACCTTT 
GGACTTCCAT CTACAAGCAG TCAGGCTCCT TCAACATTTC AGTTTGGGAC AGCGAGCCTG 
GGACAGCAGG AAAAGAAAGA GGAACCAGTT TTGGGAGGCT TTGGTTTTGG CACGAGTTCT 
ACTTCTTCCA TAGCTACCAG TGAGAATAAA ACCGGAGTCA GTGGCTTCAC TTTTGGAACT 
GTGGCAGAAA AGGAGGTAGC ATCACCTGCT CTTACATTTA AGAAGTCAGA TGAGAAAAAG 
GATGAAACTC TTTCAACGAA GGGAGGCTTC TCTTTTGGCA ACGTGGAGTC TGCACCTGCC 
TCACAGTTTG TTTTGGGAAG GACAGAAGAG AAACAGGACT TGGTCACTTC TGCTGCTCCG 
TTAGCATTTG GAAAGAAAGC TGACAGTGAA GAGTTAAAGG CACAACCTGT CTTTTCAGCT 
GGAACTGCTG AGCATACCAA AGAGGAGAGC GCAGCAAAAC CTATATTCAA CTTCAGTTTC 
GTCAAACCAT CGGAGAAGGA AACCGATCAG GCAAAGCCGT CTTTTGCGTT TGGGGCACAA 
ACCAGTACTT CAGATCAAGG AGCAGCAAAG CCATCTTTCA GTTTCTTGAG CTCAAGTTCC 
TCCAGCACAG TCGTACCCAC CACTTCAGCC AACAGCAGCA GCGTGTTTGG CAGTGCCATC 
TCTTCCTCAA ATCCTCAGCC AGTTCCCGCT CCCTTCGTGT TTGGACAACC CAGCAACACT 
GTGAGCAGCT CTGTTTTTGG CAATTCTGCA GAATCTACAG CATCTCAGTC ATTTGGCTTC 
TCTCAAGAAA ACAAACCAGC AACCACATCT TCCAGCACCG GCACGGCTCT TGCTTCATTT 
CTCTTTGGTT CGGGAGCCAG CAGTACCAAT GCAGCAAGTT CAGGCTTTAC CTTTGGAGCC 
ACAACCACAT CAAGTTCAAC AGGGTCATCT TCCTCCTTTC TGTTTGGCTC CGGGCCCCCG 
GCGCCTGCAG CCGGCCCCGC GTTTGGCGCC AGCCAGCCAC CAGCATTTGG CCAGAGCCAA 
GGCTCCGGCC AGCCCAGCGC TCCCAGTTTC GGATCGCTGT CGACGACGCT GTTCTCAGCT 
GGCGCCCAGT CTGCTCCTCC CGCCTTCGGC TCGGTGACCA GCAGCACCCA GCCCTCCGTC 
TTCGGACAGC AAGCCACCCA ACAGCCAGGG TTTGGCTCTG CTACCCCCAG CACTGGTTCC 
GTATTCCAGT TTGGAAGTAG TACTTCTAAT TTCAACTTTC CGAGTAACCC CGGCGTATTT 
ACTTTTGGTG CAAATACTCC CGCACCGGCA GCACCGGCAC AGCCTTCTGG CTCGTCAGGA 
TTTTCCTTCA ACCAGCCTCT GGCATTCACA GTGGGGACTA ATGGAAAAAA TGTTTTCTCT 
GCCTCCGGAT CTTCAGTTTC TGGTCGGAAG ATAAAAACTG CTGTTAGACG TAGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP153 ( XM_014940892.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796378.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCa64253
Clone ID Related Accession (Same CDS sequence)	XM_014940892.1
Accession Version	XM_014940892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTACAAGCC AGCTTGGGGA TTCTCCATTT TACCCTGGAA AGACGACGTA CGGTGGTGCA 
GCTGCAGCAG CAAGGGAGAC AAAAGCACGA ATTGCTCCTT ATCAGCCCCC GGTAAGAAGA 
CAAGTGAAAG CTAAACAAGC AAGTGTGCAA TCCTATGGTG TGACAAGTTC CACAGCGCGG 
CGCATCTTGC AGTCATTGGA GAAGATGTCA AGTCCTTTGG CGGATGCTAA AAGGATTCCA 
TCTTCTGTTT CTAGTCCACT GTCTTCTCCT GTGGACAGGG GTGCACAGCT GAATATTGCT 
AGCTTCCAAT CCAAACGAAA ACAGATGGAA TCGCAGCATC CACCTGTCCA GAAACTCGTG 
ACACCAAAGG CAATCTCTCT GTCAGTGAGT CGGACCCAGT ATTTTAAACC ATCTCTGACT 
CCAGCTACTG ATTCCAGCAA AATTCAACAG AGGGTAGATA TAAAGCACAA AGGCATGAGA 
GAGAAGAGTT TTCCTGCAGA ACAGCAAGTA AAACCATCTG AAAGCAATTT GACCTACCCT 
AAATTCAGTA GTCCTGCATC CAATGGTCTG TCTTCAGGAG GAGTAGGTGG TGGTGGCAAG 
ATGAGAAGGG AAAGAGGAGT ACACTATATA TCAAAACCTG GACAAGAACA AGAGGAGGTG 
GAAGAACCGG TACTACCGAA AGTACCCCTA CCCATCAGTA CTGCATCGCT GCCTTCGTTC 
AACTTTAGTT TTCTTCCCAG TAGTACTGTC TCATCCTCAC CAAGCACTGT TTCAACAGCA 
GTGATGAACA AGGTACAACC AGCAAGCAAT GTTGGCAGTC CTGTCTTCAG ATTTTCATCT 
CCAATTGTGA AATCTACTGA GGCGGAAGTG CTACCTCCGT TGTCTCAGAT TGGGTTTACA 
TTTAGTGTTC CTGTTGTAAA ATCAGCAGAG CTTTCTGGAT CCAGCGATAC ACCAGTGACG 
TCCTTACTTA CTCTAGATAC CACCACTGTA AACAGCACCA GCAATAAAAA GGAAGAAAAA 
GAAGAATATG ATGGCCTCAC CAAGCCTGCA AAAGTCTTAA AGGAAGGAAG TGTGTTAGAC 
ATTCTAAAAA GCCCTGGCTT CACGTCTGAG AAAACACACT CCTCTACATC CGCACAGCCT 
GTTACGAGCA CAGTGGTCTA TACAAGGCCT GCAATAAGTA GTTTTTCTGC AGGCAAAGAC 
AGCCCTAAAC AGCCATCCTC GTTTTGGCAG TCTGACACGC ATGACCCATG TCTGCAAAAC 
AAACCCACAG ATGGCAAATG TGTAACGTGT CAAGCTGCTA AAGTGTCAAC AGTGGAGAGT 
ACGAAACCCA CGATAAGCTC TAGTCCGTGC GTCACCTCGA AGGCCGCAGT CCCTACAGCC 
GGGACACTGG GCTTTGAAGA CAAATTTAAA CCACCACCCA ACACATGGGA TTGTGATACC 
TGTCTGGTCC AGAACAAACC TGAAGCTACA AAATGTATAG CCTGTGAGAC ACCAAAGCCT 
GGGACAGGAG TAATGCCTGC TCTGACATTG CCAGTGGTCA CAGACAGCTC GGTGACAGTG 
ACATCCTCCT CCAGCAGCAC TGACACAACG GCCACTTTGG GATTTGGAGA CAAATTTAAG 
AAGCCAAAAG GCTCTTGGGA GTGTGCAGTG TGCCTTGTAT CAAATAAGGC AGAAGACAGC 
AAATGTGTAG CCTGTCAGTC TGAGAAACCA GGGAGTTCAG TGCCTGTGAC CAGTAGCAGT 
GTTTCTACAT TTTCTGCTCC TTCAGGAGGA TTTCTGGATT TAGACAAATT CAAGAAACCT 
GAAGGAAGCT GGGATTGTGA GGTCTGCTTG GTCCAAAACA AAGCAGAAGC CACAAAATGT 
GTAGCCTGTG AAAGTGCAAA GCCAGGCACC AAAGCAGAGC TCAAAGGTTT TGGTACTCCT 
ACTGTGTCCA CAAATGCTGC AATGCCATCA TTTACATTTG GTGTCCAGTC GTCCTCCTCT 
GAATCTTCTC ATATGTTAGG TAGCACAGGA AATTTTAAAT TTGGAGAACA AGGGAGCTTC 
AAGTTTGGCG TTGCATCAGA ACCTGCGTCC TCCAACACTG TGACTGGGGG TTTTAAGTTT 
CCTACTAGTT CAGGGGACTT CAAATTTGGA GTTTCTTCCT CAGACTCTAA ATCAGAAGAC 
AGTAAAAAAG AAGGTAAAAG TAACAGTTTT ACCTTTGGAC TTCCATCTAC AAGCAGTCAG 
GCTCCTTCAA CATTTCAGTT TGGGACAGCG AGCCTGGGAC AGCAGGAAAA GAAAGAGGAA 
CCAGTTTTGG GAGGCTTTGG TTTTGGCACG AGTTCTACTT CTTCCATAGC TACCAGTGAG 
AATAAAACCG GAGTCAGTGG CTTCACTTTT GGAACTGTGG CAGAAAAGGA GGTAGCATCA 
CCTGCTCTTA CATTTAAGAA GTCAGATGAG AAAAAGGATG AAACTCTTTC AACGAAGGGA 
GGCTTCTCTT TTGGCAACGT GGAGTCTGCA CCTGCCTCAC AGTTTGTTTT GGGAAGGACA 
GAAGAGAAAC AGGACTTGGT CACTTCTGCT GCTCCGTTAG CATTTGGAAA GAAAGCTGAC 
AGTGAAGAGT TAAAGGCACA ACCTGTCTTT TCAGCTGGAA CTGCTGAGCA TACCAAAGAG 
GAGAGCGCAG CAAAACCTAT ATTCAACTTC AGTTTCGTCA AACCATCGGA GAAGGAAACC 
GATCAGGCAA AGCCGTCTTT TGCGTTTGGG GCACAAACCA GTACTTCAGA TCAAGGAGCA 
GCAAAGCCAT CTTTCAGTTT CTTGAGCTCA AGTTCCTCCA GCACAGTCGT ACCCACCACT 
TCAGCCAACA GCAGCAGCGT GTTTGGCAGT GCCATCTCTT CCTCAAATCC TCAGCCAGTT 
CCCGCTCCCT TCGTGTTTGG ACAACCCAGC AACACTGTGA GCAGCTCTGT TTTTGGCAAT 
TCTGCAGAAT CTACAGCATC TCAGTCATTT GGCTTCTCTC AAGAAAACAA ACCAGCAACC 
ACATCTTCCA GCACCGGCAC GGCTCTTGCT TCATTTCTCT TTGGTTCGGG AGCCAGCAGT 
ACCAATGCAG CAAGTTCAGG CTTTACCTTT GGAGCCACAA CCACATCAAG TTCAACAGGG 
TCATCTTCCT CCTTTCTGTT TGGCTCCGGG CCCCCGGCGC CTGCAGCCGG CCCCGCGTTT 
GGCGCCAGCC AGCCACCAGC ATTTGGCCAG AGCCAAGGCT CCGGCCAGCC CAGCGCTCCC 
AGTTTCGGAT CGCTGTCGAC GACGCTGTTC TCAGCTGGCG CCCAGTCTGC TCCTCCCGCC 
TTCGGCTCGG TGACCAGCAG CACCCAGCCC TCCGTCTTCG GACAGCAAGC CACCCAACAG 
CCAGGGTTTG GCTCTGCTAC CCCCAGCACT GGTTCCGTAT TCCAGTTTGG AAGTAGTACT 
TCTAATTTCA ACTTTCCGAG TAACCCCGGC GTATTTACTT TTGGTGCAAA TACTCCCGCA 
CCGGCAGCAC CGGCACAGCC TTCTGGCTCG TCAGGATTTT CCTTCAACCA GCCTCTGGCA 
TTCACAGTGG GGACTAATGG AAAAAATGTT TTCTCTGCCT CCGGATCTTC AGTTTCTGGT 
CGGAAGATAA AAACTGCTGT TAGACGTAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796378.1
CDS	1..4356
Translation	MASGGGGGGGGGGVGGGGKIRTRRYHLGAAKPPYVRSKQQGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQMESQHPPVQKLVTPKAISLSVSRTQYFKPSLTPATDSSKIQQRVDIKHKGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSQIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940892.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940892.1

ORF Insert Sequence:

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTACAAGCC AGCTTGGGGA TTCTCCATTT TACCCTGGAA AGACGACGTA CGGTGGTGCA 
GCTGCAGCAG CAAGGGAGAC AAAAGCACGA ATTGCTCCTT ATCAGCCCCC GGTAAGAAGA 
CAAGTGAAAG CTAAACAAGC AAGTGTGCAA TCCTATGGTG TGACAAGTTC CACAGCGCGG 
CGCATCTTGC AGTCATTGGA GAAGATGTCA AGTCCTTTGG CGGATGCTAA AAGGATTCCA 
TCTTCTGTTT CTAGTCCACT GTCTTCTCCT GTGGACAGGG GTGCACAGCT GAATATTGCT 
AGCTTCCAAT CCAAACGAAA ACAGATGGAA TCGCAGCATC CACCTGTCCA GAAACTCGTG 
ACACCAAAGG CAATCTCTCT GTCAGTGAGT CGGACCCAGT ATTTTAAACC ATCTCTGACT 
CCAGCTACTG ATTCCAGCAA AATTCAACAG AGGGTAGATA TAAAGCACAA AGGCATGAGA 
GAGAAGAGTT TTCCTGCAGA ACAGCAAGTA AAACCATCTG AAAGCAATTT GACCTACCCT 
AAATTCAGTA GTCCTGCATC CAATGGTCTG TCTTCAGGAG GAGTAGGTGG TGGTGGCAAG 
ATGAGAAGGG AAAGAGGAGT ACACTATATA TCAAAACCTG GACAAGAACA AGAGGAGGTG 
GAAGAACCGG TACTACCGAA AGTACCCCTA CCCATCAGTA CTGCATCGCT GCCTTCGTTC 
AACTTTAGTT TTCTTCCCAG TAGTACTGTC TCATCCTCAC CAAGCACTGT TTCAACAGCA 
GTGATGAACA AGGTACAACC AGCAAGCAAT GTTGGCAGTC CTGTCTTCAG ATTTTCATCT 
CCAATTGTGA AATCTACTGA GGCGGAAGTG CTACCTCCGT TGTCTCAGAT TGGGTTTACA 
TTTAGTGTTC CTGTTGTAAA ATCAGCAGAG CTTTCTGGAT CCAGCGATAC ACCAGTGACG 
TCCTTACTTA CTCTAGATAC CACCACTGTA AACAGCACCA GCAATAAAAA GGAAGAAAAA 
GAAGAATATG ATGGCCTCAC CAAGCCTGCA AAAGTCTTAA AGGAAGGAAG TGTGTTAGAC 
ATTCTAAAAA GCCCTGGCTT CACGTCTGAG AAAACACACT CCTCTACATC CGCACAGCCT 
GTTACGAGCA CAGTGGTCTA TACAAGGCCT GCAATAAGTA GTTTTTCTGC AGGCAAAGAC 
AGCCCTAAAC AGCCATCCTC GTTTTGGCAG TCTGACACGC ATGACCCATG TCTGCAAAAC 
AAACCCACAG ATGGCAAATG TGTAACGTGT CAAGCTGCTA AAGTGTCAAC AGTGGAGAGT 
ACGAAACCCA CGATAAGCTC TAGTCCGTGC GTCACCTCGA AGGCCGCAGT CCCTACAGCC 
GGGACACTGG GCTTTGAAGA CAAATTTAAA CCACCACCCA ACACATGGGA TTGTGATACC 
TGTCTGGTCC AGAACAAACC TGAAGCTACA AAATGTATAG CCTGTGAGAC ACCAAAGCCT 
GGGACAGGAG TAATGCCTGC TCTGACATTG CCAGTGGTCA CAGACAGCTC GGTGACAGTG 
ACATCCTCCT CCAGCAGCAC TGACACAACG GCCACTTTGG GATTTGGAGA CAAATTTAAG 
AAGCCAAAAG GCTCTTGGGA GTGTGCAGTG TGCCTTGTAT CAAATAAGGC AGAAGACAGC 
AAATGTGTAG CCTGTCAGTC TGAGAAACCA GGGAGTTCAG TGCCTGTGAC CAGTAGCAGT 
GTTTCTACAT TTTCTGCTCC TTCAGGAGGA TTTCTGGATT TAGACAAATT CAAGAAACCT 
GAAGGAAGCT GGGATTGTGA GGTCTGCTTG GTCCAAAACA AAGCAGAAGC CACAAAATGT 
GTAGCCTGTG AAAGTGCAAA GCCAGGCACC AAAGCAGAGC TCAAAGGTTT TGGTACTCCT 
ACTGTGTCCA CAAATGCTGC AATGCCATCA TTTACATTTG GTGTCCAGTC GTCCTCCTCT 
GAATCTTCTC ATATGTTAGG TAGCACAGGA AATTTTAAAT TTGGAGAACA AGGGAGCTTC 
AAGTTTGGCG TTGCATCAGA ACCTGCGTCC TCCAACACTG TGACTGGGGG TTTTAAGTTT 
CCTACTAGTT CAGGGGACTT CAAATTTGGA GTTTCTTCCT CAGACTCTAA ATCAGAAGAC 
AGTAAAAAAG AAGGTAAAAG TAACAGTTTT ACCTTTGGAC TTCCATCTAC AAGCAGTCAG 
GCTCCTTCAA CATTTCAGTT TGGGACAGCG AGCCTGGGAC AGCAGGAAAA GAAAGAGGAA 
CCAGTTTTGG GAGGCTTTGG TTTTGGCACG AGTTCTACTT CTTCCATAGC TACCAGTGAG 
AATAAAACCG GAGTCAGTGG CTTCACTTTT GGAACTGTGG CAGAAAAGGA GGTAGCATCA 
CCTGCTCTTA CATTTAAGAA GTCAGATGAG AAAAAGGATG AAACTCTTTC AACGAAGGGA 
GGCTTCTCTT TTGGCAACGT GGAGTCTGCA CCTGCCTCAC AGTTTGTTTT GGGAAGGACA 
GAAGAGAAAC AGGACTTGGT CACTTCTGCT GCTCCGTTAG CATTTGGAAA GAAAGCTGAC 
AGTGAAGAGT TAAAGGCACA ACCTGTCTTT TCAGCTGGAA CTGCTGAGCA TACCAAAGAG 
GAGAGCGCAG CAAAACCTAT ATTCAACTTC AGTTTCGTCA AACCATCGGA GAAGGAAACC 
GATCAGGCAA AGCCGTCTTT TGCGTTTGGG GCACAAACCA GTACTTCAGA TCAAGGAGCA 
GCAAAGCCAT CTTTCAGTTT CTTGAGCTCA AGTTCCTCCA GCACAGTCGT ACCCACCACT 
TCAGCCAACA GCAGCAGCGT GTTTGGCAGT GCCATCTCTT CCTCAAATCC TCAGCCAGTT 
CCCGCTCCCT TCGTGTTTGG ACAACCCAGC AACACTGTGA GCAGCTCTGT TTTTGGCAAT 
TCTGCAGAAT CTACAGCATC TCAGTCATTT GGCTTCTCTC AAGAAAACAA ACCAGCAACC 
ACATCTTCCA GCACCGGCAC GGCTCTTGCT TCATTTCTCT TTGGTTCGGG AGCCAGCAGT 
ACCAATGCAG CAAGTTCAGG CTTTACCTTT GGAGCCACAA CCACATCAAG TTCAACAGGG 
TCATCTTCCT CCTTTCTGTT TGGCTCCGGG CCCCCGGCGC CTGCAGCCGG CCCCGCGTTT 
GGCGCCAGCC AGCCACCAGC ATTTGGCCAG AGCCAAGGCT CCGGCCAGCC CAGCGCTCCC 
AGTTTCGGAT CGCTGTCGAC GACGCTGTTC TCAGCTGGCG CCCAGTCTGC TCCTCCCGCC 
TTCGGCTCGG TGACCAGCAG CACCCAGCCC TCCGTCTTCG GACAGCAAGC CACCCAACAG 
CCAGGGTTTG GCTCTGCTAC CCCCAGCACT GGTTCCGTAT TCCAGTTTGG AAGTAGTACT 
TCTAATTTCA ACTTTCCGAG TAACCCCGGC GTATTTACTT TTGGTGCAAA TACTCCCGCA 
CCGGCAGCAC CGGCACAGCC TTCTGGCTCG TCAGGATTTT CCTTCAACCA GCCTCTGGCA 
TTCACAGTGG GGACTAATGG AAAAAATGTT TTCTCTGCCT CCGGATCTTC AGTTTCTGGT 
CGGAAGATAA AAACTGCTGT TAGACGTAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP153 ( XM_014940894.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014796380.1 Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCa64255
Clone ID Related Accession (Same CDS sequence)	XM_014940894.1
Accession Version	XM_014940894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup153 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GGGCATGAGA 
GAGAAGAGTT TTCCTGCAGA ACAGCAAGTA AAACCATCTG AAAGCAATTT GACCTACCCT 
AAATTCAGTA GTCCTGCATC CAATGGTCTG TCTTCAGGAG GAGTAGGTGG TGGTGGCAAG 
ATGAGAAGGG AAAGAGGAGT ACACTATATA TCAAAACCTG GACAAGAACA AGAGGAGGTG 
GAAGAACCGG TACTACCGAA AGTACCCCTA CCCATCAGTA CTGCATCGCT GCCTTCGTTC 
AACTTTAGTT TTCTTCCCAG TAGTACTGTC TCATCCTCAC CAAGCACTGT TTCAACAGCA 
GTGATGAACA AGGTACAACC AGCAAGCAAT GTTGGCAGTC CTGTCTTCAG ATTTTCATCT 
CCAATTGTGA AATCTACTGA GGCGGAAGTG CTACCTCCGT TGTCTCAGAT TGGGTTTACA 
TTTAGTGTTC CTGTTGTAAA ATCAGCAGAG CTTTCTGGAT CCAGCGATAC ACCAGTGACG 
TCCTTACTTA CTCTAGATAC CACCACTGTA AACAGCACCA GCAATAAAAA GGAAGAAAAA 
GAAGAATATG ATGGCCTCAC CAAGCCTGCA AAAGTCTTAA AGGAAGGAAG TGTGTTAGAC 
ATTCTAAAAA GCCCTGGCTT CACGTCTGAG AAAACACACT CCTCTACATC CGCACAGCCT 
GTTACGAGCA CAGTGGTCTA TACAAGGCCT GCAATAAGTA GTTTTTCTGC AGGCAAAGAC 
AGCCCTAAAC AGCCATCCTC GTTTTGGCAG TCTGACACGC ATGACCCATG TCTGCAAAAC 
AAACCCACAG ATGGCAAATG TGTAACGTGT CAAGCTGCTA AAGTGTCAAC AGTGGAGAGT 
ACGAAACCCA CGATAAGCTC TAGTCCGTGC GTCACCTCGA AGGCCGCAGT CCCTACAGCC 
GGGACACTGG GCTTTGAAGA CAAATTTAAA CCACCACCCA ACACATGGGA TTGTGATACC 
TGTCTGGTCC AGAACAAACC TGAAGCTACA AAATGTATAG CCTGTGAGAC ACCAAAGCCT 
GGGACAGGAG TAATGCCTGC TCTGACATTG CCAGTGGTCA CAGACAGCTC GGTGACAGTG 
ACATCCTCCT CCAGCAGCAC TGACACAACG GCCACTTTGG GATTTGGAGA CAAATTTAAG 
AAGCCAAAAG GCTCTTGGGA GTGTGCAGTG TGCCTTGTAT CAAATAAGGC AGAAGACAGC 
AAATGTGTAG CCTGTCAGTC TGAGAAACCA GGGAGTTCAG TGCCTGTGAC CAGTAGCAGT 
GTTTCTACAT TTTCTGCTCC TTCAGGAGGA TTTCTGGATT TAGACAAATT CAAGAAACCT 
GAAGGAAGCT GGGATTGTGA GGTCTGCTTG GTCCAAAACA AAGCAGAAGC CACAAAATGT 
GTAGCCTGTG AAAGTGCAAA GCCAGGCACC AAAGCAGAGC TCAAAGGTTT TGGTACTCCT 
ACTGTGTCCA CAAATGCTGC AATGCCATCA TTTACATTTG GTGTCCAGTC GTCCTCCTCT 
GAATCTTCTC ATATGTTAGG TAGCACAGGA AATTTTAAAT TTGGAGAACA AGGGAGCTTC 
AAGTTTGGCG TTGCATCAGA ACCTGCGTCC TCCAACACTG TGACTGGGGG TTTTAAGTTT 
CCTACTAGTT CAGGGGACTT CAAATTTGGA GTTTCTTCCT CAGACTCTAA ATCAGAAGAC 
AGTAAAAAAG AAGGTAAAAG TAACAGTTTT ACCTTTGGAC TTCCATCTAC AAGCAGTCAG 
GCTCCTTCAA CATTTCAGTT TGGGACAGCG AGCCTGGGAC AGCAGGAAAA GAAAGAGGAA 
CCAGTTTTGG GAGGCTTTGG TTTTGGCACG AGTTCTACTT CTTCCATAGC TACCAGTGAG 
AATAAAACCG GAGTCAGTGG CTTCACTTTT GGAACTGTGG CAGAAAAGGA GGTAGCATCA 
CCTGCTCTTA CATTTAAGAA GTCAGATGAG AAAAAGGATG AAACTCTTTC AACGAAGGGA 
GGCTTCTCTT TTGGCAACGT GGAGTCTGCA CCTGCCTCAC AGTTTGTTTT GGGAAGGACA 
GAAGAGAAAC AGGACTTGGT CACTTCTGCT GCTCCGTTAG CATTTGGAAA GAAAGCTGAC 
AGTGAAGAGT TAAAGGCACA ACCTGTCTTT TCAGCTGGAA CTGCTGAGCA TACCAAAGAG 
GAGAGCGCAG CAAAACCTAT ATTCAACTTC AGTTTCGTCA AACCATCGGA GAAGGAAACC 
GATCAGGCAA AGCCGTCTTT TGCGTTTGGG GCACAAACCA GTACTTCAGA TCAAGGAGCA 
GCAAAGCCAT CTTTCAGTTT CTTGAGCTCA AGTTCCTCCA GCACAGTCGT ACCCACCACT 
TCAGCCAACA GCAGCAGCGT GTTTGGCAGT GCCATCTCTT CCTCAAATCC TCAGCCAGTT 
CCCGCTCCCT TCGTGTTTGG ACAACCCAGC AACACTGTGA GCAGCTCTGT TTTTGGCAAT 
TCTGCAGAAT CTACAGCATC TCAGTCATTT GGCTTCTCTC AAGAAAACAA ACCAGCAACC 
ACATCTTCCA GCACCGGCAC GGCTCTTGCT TCATTTCTCT TTGGTTCGGG AGCCAGCAGT 
ACCAATGCAG CAAGTTCAGG CTTTACCTTT GGAGCCACAA CCACATCAAG TTCAACAGGG 
TCATCTTCCT CCTTTCTGTT TGGCTCCGGG CCCCCGGCGC CTGCAGCCGG CCCCGCGTTT 
GGCGCCAGCC AGCCACCAGC ATTTGGCCAG AGCCAAGGCT CCGGCCAGCC CAGCGCTCCC 
AGTTTCGGAT CGCTGTCGAC GACGCTGTTC TCAGCTGGCG CCCAGTCTGC TCCTCCCGCC 
TTCGGCTCGG TGACCAGCAG CACCCAGCCC TCCGTCTTCG GACAGCAAGC CACCCAACAG 
CCAGGGTTTG GCTCTGCTAC CCCCAGCACT GGTTCCGTAT TCCAGTTTGG AAGTAGTACT 
TCTAATTTCA ACTTTCCGAG TAACCCCGGC GTATTTACTT TTGGTGCAAA TACTCCCGCA 
CCGGCAGCAC CGGCACAGCC TTCTGGCTCG TCAGGATTTT CCTTCAACCA GCCTCTGGCA 
TTCACAGTGG GGACTAATGG AAAAAATGTT TTCTCTGCCT CCGGATCTTC AGTTTCTGGT 
CGGAAGATAA AAACTGCTGT TAGACGTAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014796380.1
CDS	1..4236
Translation	MASGGGGGGGGGGVGGGGKIRTRRYHLGAAKPPYVRSKQQGIISRVTESVKNIVPGWLQKYFNRSEDECVDTNESTNQEESPVNYHHDYANEDTIVIDGRVTPESARINLEEPSTSRSALNFSDVLTRPSLHRSHLNCTMLDSTVPHCQPSTSSSLGIGSPGLSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSAPLLWSTEAERSHSLSQHSATSSKKPAFNLSAFGSPSPSLGNTSVFKTSQLGDSPFYPGKTTYGGAAAAARETKARIAPYQPPVRRQVKAKQASVQSYGVTSSTARRILQSLEKMSSPLADAKRIPSSVSSPLSSPVDRGAQLNIASFQSKRKQGMREKSFPAEQQVKPSESNLTYPKFSSPASNGLSSGGVGGGGKMRRERGVHYISKPGQEQEEVEEPVLPKVPLPISTASLPSFNFSFLPSSTVSSSPSTVSTAVMNKVQPASNVGSPVFRFSSPIVKSTEAEVLPPLSQIGFTFSVPVVKSAELSGSSDTPVTSLLTLDTTTVNSTSNKKEEKEEYDGLTKPAKVLKEGSVLDILKSPGFTSEKTHSSTSAQPVTSTVVYTRPAISSFSAGKDSPKQPSSFWQSDTHDPCLQNKPTDGKCVTCQAAKVSTVESTKPTISSSPCVTSKAAVPTAGTLGFEDKFKPPPNTWDCDTCLVQNKPEATKCIACETPKPGTGVMPALTLPVVTDSSVTVTSSSSSTDTTATLGFGDKFKKPKGSWECAVCLVSNKAEDSKCVACQSEKPGSSVPVTSSSVSTFSAPSGGFLDLDKFKKPEGSWDCEVCLVQNKAEATKCVACESAKPGTKAELKGFGTPTVSTNAAMPSFTFGVQSSSSESSHMLGSTGNFKFGEQGSFKFGVASEPASSNTVTGGFKFPTSSGDFKFGVSSSDSKSEDSKKEGKSNSFTFGLPSTSSQAPSTFQFGTASLGQQEKKEEPVLGGFGFGTSSTSSIATSENKTGVSGFTFGTVAEKEVASPALTFKKSDEKKDETLSTKGGFSFGNVESAPASQFVLGRTEEKQDLVTSAAPLAFGKKADSEELKAQPVFSAGTAEHTKEESAAKPIFNFSFVKPSEKETDQAKPSFAFGAQTSTSDQGAAKPSFSFLSSSSSSTVVPTTSANSSSVFGSAISSSNPQPVPAPFVFGQPSNTVSSSVFGNSAESTASQSFGFSQENKPATTSSSTGTALASFLFGSGASSTNAASSGFTFGATTTSSSTGSSSSFLFGSGPPAPAAGPAFGASQPPAFGQSQGSGQPSAPSFGSLSTTLFSAGAQSAPPAFGSVTSSTQPSVFGQQATQQPGFGSATPSTGSVFQFGSSTSNFNFPSNPGVFTFGANTPAPAAPAQPSGSSGFSFNQPLAFTVGTNGKNVFSASGSSVSGRKIKTAVRRRK

Target ORF information:

RefSeq Version	XM_014940894.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 153kDa (NUP153), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014940894.1

ORF Insert Sequence:

ATGGCCTCGG GAGGCGGCGG CGGCGGCGGC GGCGGGGGGG TAGGCGGCGG CGGCAAGATC 
CGGACCCGGC GGTACCACCT GGGCGCCGCG AAGCCTCCCT ACGTCCGGAG TAAGCAGCAG 
GGAATCATTA GCAGAGTTAC AGAATCGGTG AAAAATATTG TACCGGGATG GTTACAGAAG 
TATTTCAATA GAAGTGAAGA TGAATGTGTA GATACAAATG AATCTACAAA CCAGGAAGAA 
AGTCCAGTAA ACTATCACCA TGATTATGCA AATGAAGATA CCATAGTGAT TGATGGGAGA 
GTCACGCCTG AATCAGCAAG AATTAATTTA GAAGAACCAT CCACGAGTAG ATCTGCATTG 
AACTTCTCAG ATGTGTTAAC AAGGCCCTCT CTTCATCGGA GTCATTTGAA TTGTACCATG 
TTGGATTCCA CAGTACCACA TTGTCAGCCC TCTACATCTT CTTCACTTGG CATTGGCAGT 
CCTGGACTGT CTCTTGTAAA AGAAATAAAA GATTCTACCT CTCAGCATGA TGACGATAAC 
ATCTCAACAA CCAGTGGCTT CTCCTCTAGA GCATCTGATA AAGATATCAC AGTTTCAAAA 
AATACTTCAG CGCCGCTGCT CTGGTCCACA GAGGCTGAAA GATCTCATTC CCTCTCACAG 
CATTCTGCAA CTAGCTCTAA AAAACCTGCA TTCAATTTAT CTGCTTTTGG ATCTCCCTCT 
CCTTCACTTG GAAACACTTC TGTCTTTAAG ACAAGCCAGC TTGGGGATTC TCCATTTTAC 
CCTGGAAAGA CGACGTACGG TGGTGCAGCT GCAGCAGCAA GGGAGACAAA AGCACGAATT 
GCTCCTTATC AGCCCCCGGT AAGAAGACAA GTGAAAGCTA AACAAGCAAG TGTGCAATCC 
TATGGTGTGA CAAGTTCCAC AGCGCGGCGC ATCTTGCAGT CATTGGAGAA GATGTCAAGT 
CCTTTGGCGG ATGCTAAAAG GATTCCATCT TCTGTTTCTA GTCCACTGTC TTCTCCTGTG 
GACAGGGGTG CACAGCTGAA TATTGCTAGC TTCCAATCCA AACGAAAACA GGGCATGAGA 
GAGAAGAGTT TTCCTGCAGA ACAGCAAGTA AAACCATCTG AAAGCAATTT GACCTACCCT 
AAATTCAGTA GTCCTGCATC CAATGGTCTG TCTTCAGGAG GAGTAGGTGG TGGTGGCAAG 
ATGAGAAGGG AAAGAGGAGT ACACTATATA TCAAAACCTG GACAAGAACA AGAGGAGGTG 
GAAGAACCGG TACTACCGAA AGTACCCCTA CCCATCAGTA CTGCATCGCT GCCTTCGTTC 
AACTTTAGTT TTCTTCCCAG TAGTACTGTC TCATCCTCAC CAAGCACTGT TTCAACAGCA 
GTGATGAACA AGGTACAACC AGCAAGCAAT GTTGGCAGTC CTGTCTTCAG ATTTTCATCT 
CCAATTGTGA AATCTACTGA GGCGGAAGTG CTACCTCCGT TGTCTCAGAT TGGGTTTACA 
TTTAGTGTTC CTGTTGTAAA ATCAGCAGAG CTTTCTGGAT CCAGCGATAC ACCAGTGACG 
TCCTTACTTA CTCTAGATAC CACCACTGTA AACAGCACCA GCAATAAAAA GGAAGAAAAA 
GAAGAATATG ATGGCCTCAC CAAGCCTGCA AAAGTCTTAA AGGAAGGAAG TGTGTTAGAC 
ATTCTAAAAA GCCCTGGCTT CACGTCTGAG AAAACACACT CCTCTACATC CGCACAGCCT 
GTTACGAGCA CAGTGGTCTA TACAAGGCCT GCAATAAGTA GTTTTTCTGC AGGCAAAGAC 
AGCCCTAAAC AGCCATCCTC GTTTTGGCAG TCTGACACGC ATGACCCATG TCTGCAAAAC 
AAACCCACAG ATGGCAAATG TGTAACGTGT CAAGCTGCTA AAGTGTCAAC AGTGGAGAGT 
ACGAAACCCA CGATAAGCTC TAGTCCGTGC GTCACCTCGA AGGCCGCAGT CCCTACAGCC 
GGGACACTGG GCTTTGAAGA CAAATTTAAA CCACCACCCA ACACATGGGA TTGTGATACC 
TGTCTGGTCC AGAACAAACC TGAAGCTACA AAATGTATAG CCTGTGAGAC ACCAAAGCCT 
GGGACAGGAG TAATGCCTGC TCTGACATTG CCAGTGGTCA CAGACAGCTC GGTGACAGTG 
ACATCCTCCT CCAGCAGCAC TGACACAACG GCCACTTTGG GATTTGGAGA CAAATTTAAG 
AAGCCAAAAG GCTCTTGGGA GTGTGCAGTG TGCCTTGTAT CAAATAAGGC AGAAGACAGC 
AAATGTGTAG CCTGTCAGTC TGAGAAACCA GGGAGTTCAG TGCCTGTGAC CAGTAGCAGT 
GTTTCTACAT TTTCTGCTCC TTCAGGAGGA TTTCTGGATT TAGACAAATT CAAGAAACCT 
GAAGGAAGCT GGGATTGTGA GGTCTGCTTG GTCCAAAACA AAGCAGAAGC CACAAAATGT 
GTAGCCTGTG AAAGTGCAAA GCCAGGCACC AAAGCAGAGC TCAAAGGTTT TGGTACTCCT 
ACTGTGTCCA CAAATGCTGC AATGCCATCA TTTACATTTG GTGTCCAGTC GTCCTCCTCT 
GAATCTTCTC ATATGTTAGG TAGCACAGGA AATTTTAAAT TTGGAGAACA AGGGAGCTTC 
AAGTTTGGCG TTGCATCAGA ACCTGCGTCC TCCAACACTG TGACTGGGGG TTTTAAGTTT 
CCTACTAGTT CAGGGGACTT CAAATTTGGA GTTTCTTCCT CAGACTCTAA ATCAGAAGAC 
AGTAAAAAAG AAGGTAAAAG TAACAGTTTT ACCTTTGGAC TTCCATCTAC AAGCAGTCAG 
GCTCCTTCAA CATTTCAGTT TGGGACAGCG AGCCTGGGAC AGCAGGAAAA GAAAGAGGAA 
CCAGTTTTGG GAGGCTTTGG TTTTGGCACG AGTTCTACTT CTTCCATAGC TACCAGTGAG 
AATAAAACCG GAGTCAGTGG CTTCACTTTT GGAACTGTGG CAGAAAAGGA GGTAGCATCA 
CCTGCTCTTA CATTTAAGAA GTCAGATGAG AAAAAGGATG AAACTCTTTC AACGAAGGGA 
GGCTTCTCTT TTGGCAACGT GGAGTCTGCA CCTGCCTCAC AGTTTGTTTT GGGAAGGACA 
GAAGAGAAAC AGGACTTGGT CACTTCTGCT GCTCCGTTAG CATTTGGAAA GAAAGCTGAC 
AGTGAAGAGT TAAAGGCACA ACCTGTCTTT TCAGCTGGAA CTGCTGAGCA TACCAAAGAG 
GAGAGCGCAG CAAAACCTAT ATTCAACTTC AGTTTCGTCA AACCATCGGA GAAGGAAACC 
GATCAGGCAA AGCCGTCTTT TGCGTTTGGG GCACAAACCA GTACTTCAGA TCAAGGAGCA 
GCAAAGCCAT CTTTCAGTTT CTTGAGCTCA AGTTCCTCCA GCACAGTCGT ACCCACCACT 
TCAGCCAACA GCAGCAGCGT GTTTGGCAGT GCCATCTCTT CCTCAAATCC TCAGCCAGTT 
CCCGCTCCCT TCGTGTTTGG ACAACCCAGC AACACTGTGA GCAGCTCTGT TTTTGGCAAT 
TCTGCAGAAT CTACAGCATC TCAGTCATTT GGCTTCTCTC AAGAAAACAA ACCAGCAACC 
ACATCTTCCA GCACCGGCAC GGCTCTTGCT TCATTTCTCT TTGGTTCGGG AGCCAGCAGT 
ACCAATGCAG CAAGTTCAGG CTTTACCTTT GGAGCCACAA CCACATCAAG TTCAACAGGG 
TCATCTTCCT CCTTTCTGTT TGGCTCCGGG CCCCCGGCGC CTGCAGCCGG CCCCGCGTTT 
GGCGCCAGCC AGCCACCAGC ATTTGGCCAG AGCCAAGGCT CCGGCCAGCC CAGCGCTCCC 
AGTTTCGGAT CGCTGTCGAC GACGCTGTTC TCAGCTGGCG CCCAGTCTGC TCCTCCCGCC 
TTCGGCTCGG TGACCAGCAG CACCCAGCCC TCCGTCTTCG GACAGCAAGC CACCCAACAG 
CCAGGGTTTG GCTCTGCTAC CCCCAGCACT GGTTCCGTAT TCCAGTTTGG AAGTAGTACT 
TCTAATTTCA ACTTTCCGAG TAACCCCGGC GTATTTACTT TTGGTGCAAA TACTCCCGCA 
CCGGCAGCAC CGGCACAGCC TTCTGGCTCG TCAGGATTTT CCTTCAACCA GCCTCTGGCA 
TTCACAGTGG GGACTAATGG AAAAAATGTT TTCTCTGCCT CCGGATCTTC AGTTTCTGGT 
CGGAAGATAA AAACTGCTGT TAGACGTAGA AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NUP153 cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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