NUP214 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP214
Entrez Gene ID	106888640
Full Name	nucleoporin 214
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014943015.1	XP_014798501.1	nuclear pore complex protein Nup214 isoform X2
XM_014943014.1	XP_014798500.1	nuclear pore complex protein Nup214 isoform X1
XM_014943016.1	XP_014798502.1	nuclear pore complex protein Nup214 isoform X3

Pathways from BioSystems
Gene Ontology

The following NUP214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa65773	XM_014943014.1 Latest version!	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa65773	XM_014943015.1 Latest version!	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa65774	XM_014943016.1 Latest version!	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP214 ( XM_014943015.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014798501.1 Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

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CloneID	OCa65773
Clone ID Related Accession (Same CDS sequence)	XM_014943015.1 , XM_014943014.1
Accession Version	XM_014943015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090846.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCAG CCATCTTCTT CCAGTGGAGG CTTGTTTGGA 
GCTGGAAGTG GTGGGAGAAG TGGAGGCTTC TTCAGTGGTT TGGGAGGGAA GCCAAGTCAG 
GATGCGGCCA ATAAGAATCC CTTCAGTTCC TCCAGTGGAG GATTTGGATC TGCAGCTTCC 
CAGAATACTT CTAGCTTGTT TGGAAACAGC GGAGCAAAGA CCTTTGGATT TGGCAGCTCA 
TCTTTTGGAG AGCAGAAGCC TACAGGCACT TTCAGCTCTG GTGGTGGAAG TGTGGCATCT 
CAAGGCTTTG GATTCTCCAG CCCAACCAAA GCAGGTGGCT TCGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTCGG GGGATCCCCT GGGTTCGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCAACCT TTTCAAGCCC TCTGGGCTCA ACGGGAGGCA AAGTGTTCGG CGAGGGTACA 
GCAGCAGCCA GCGCTGGAGG ATTTGGGTTT GGTGGTACCA GTAACAATAC AGCATCCTTT 
GGCACGCTAG CAAATCAAAA CACCCCGACG TTTGGATCAC TGTCACAACA GGGTACCGGT 
TTTGGCACAC AAAGCAGTGG ATTCTCAGCT TTTGGGACAG GTGGAGGAGG GTTTGGTTTT 
GGATCATCTA ACACATCTTC CTCTGGATTT AGTGGCTGGA GAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014798501.1
CDS	78..6365
Translation	MGDDMYVIPEREMKDFQFRALKKVRIFDAPEDLPKERSNLLAVSNKYGLVFAGGGTSLQIFHTRNLLMQNQPGEDPNKIVENVQSTSVPMKFPVQHLALSCDNLTLSVCMMSSEIGSFIGFYDVRTLLNQAKQQKRAFAYHKLAKDSGSAATVNDLKWNPTSPTMVAICLSDGSISVLQVTDSVKVYATLPSSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPSLQEKKVIPCPPFYYETDNPVKVLDVLWIGTYVFTIVYAAADGTLETPPEVVIAILPKKEEKRPEKFVNFIEPCYGSCTERQHHYFLNYVEEWDLVLAASAASTEVSILARQGDQNWELWVLEDSSRAELPVTDKSDDTLPVGVAVDYTSNMPIPISDEKTLAPAPVLILLSTDGVLCPFYMINQNPGVRSLIVTPEPLPLGGERVPKSAGSSATTPTTPPTSSKPDTGPGVAAAAAPASSQPPAGSGAAAFSFMPSAVKMPGSTGSDPPPYPFTTDASKQSLAPSLTAAATFSFSSASSKASVTPAPSVGTTTATTTSFSFASMNFKPAAESSPGPPLSTTPVAQKSSFASAASSVKVNLNEKFSAVDTSSPTSSSGQSTSTMFSFSTTSKTASGGPLSQSMPLPASPPTPLKASTPVPAAVARPAQSMPSASLLQKTAKISPSAAKPSSAQGKSALPSAPALEKQAHQWKDSDPVIAGIREEIAHFQKELEELKARTSKACFQVGTPEEMKMLRTESDDLHTFLLEIKETTESLHGDISILKTTLLEGFAGVEEAREQNERNHSSGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKRKQKRLIVPERETLFNTLANNREIINQQRKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSLSSAQSFDSDLESLRNALMKTTLESHAKPLPKVPAKLSPVKQAQLRNFLAKRKTPPIRSIAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENDDSQAVDQEEEAAPVQIPRHAPVVRTPSIQPGLMGQSPSFGKQPLTVGSSLPSSSSRIIPQGADSTMLATKTVKHGAPAPSVTVPAPQAAAAAALRRQMANQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSAVIGSSVPHSAAQAVHQVLATVATNQAKQTPLTSSLKGQTPPSSVCAPCPMASTSGPTSSGNKISGQGTVKTVTAVTSSAVTSVQTTSAQLNKAFSFSTVGSGFNFGAVTSAASSPTLSSLASSQAPAKESTQTESFSLAGGSKFAVVPESNFTFGSLKPASAPGTLVGNSSSADGVQSNKPAATTSSAPSTTSGKLDIPPSKPDHLFQSNLAGETLGSFSGLRVGQADDNAKTTTKAPSTSVAGAQSTKVSTIPAGFNFGAPLSLGKAGEAASTSATSTSMAPPSISTSATGSLFGNVQITSTGSSGVFNLGGSSGNKPTTFSFGVQQANSMSTSTSTPSTLSASPSLSFGNFLSSSQTAVPAATSSKVAGDAKLPPVSEKPSENEAVAAGSSPPMTQSPQTQVQLPESVKEPTLPQATAGDTTSAGPVATSAAPAPDAIAAPASTSDVKAPVPPAVSASTPPDQNVSATTSAANIASPAEATSVLAATSDVTTSVQSPAGGASVFAPTSAGSSVFAQPPSSSSSGSAFSQPAGDTTATPSTPPVFGQLPAGTTASSPFGQLTTSTLSTATATTQAAASSGFGTSAFGTTTTGGFGQPTFGQAPTFGQPASSSSGGFTFSQSAFGTMPAFGQPTASTAASSSGNVFGASASTNSASSFSFGQPSASTGGGLFGQTSPPVFGQSTGFGQTASVFGSAAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGSTVFGQQPSSSSGGLFGAGSGGRSGGFFSGLGGKPSQDAANKNPFSSSSGGFGSAASQNTSSLFGNSGAKTFGFGSSSFGEQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFGTLANQNTPTFGSLSQQGTGFGTQSSGFSAFGTGGGGFGFGSSNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_014943015.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014943015.1

ORF Insert Sequence:

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCAG CCATCTTCTT CCAGTGGAGG CTTGTTTGGA 
GCTGGAAGTG GTGGGAGAAG TGGAGGCTTC TTCAGTGGTT TGGGAGGGAA GCCAAGTCAG 
GATGCGGCCA ATAAGAATCC CTTCAGTTCC TCCAGTGGAG GATTTGGATC TGCAGCTTCC 
CAGAATACTT CTAGCTTGTT TGGAAACAGC GGAGCAAAGA CCTTTGGATT TGGCAGCTCA 
TCTTTTGGAG AGCAGAAGCC TACAGGCACT TTCAGCTCTG GTGGTGGAAG TGTGGCATCT 
CAAGGCTTTG GATTCTCCAG CCCAACCAAA GCAGGTGGCT TCGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTCGG GGGATCCCCT GGGTTCGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCAACCT TTTCAAGCCC TCTGGGCTCA ACGGGAGGCA AAGTGTTCGG CGAGGGTACA 
GCAGCAGCCA GCGCTGGAGG ATTTGGGTTT GGTGGTACCA GTAACAATAC AGCATCCTTT 
GGCACGCTAG CAAATCAAAA CACCCCGACG TTTGGATCAC TGTCACAACA GGGTACCGGT 
TTTGGCACAC AAAGCAGTGG ATTCTCAGCT TTTGGGACAG GTGGAGGAGG GTTTGGTTTT 
GGATCATCTA ACACATCTTC CTCTGGATTT AGTGGCTGGA GAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_014943014.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014798500.1 Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

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CloneID	OCa65773
Clone ID Related Accession (Same CDS sequence)	XM_014943015.1 , XM_014943014.1
Accession Version	XM_014943014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090846.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCAG CCATCTTCTT CCAGTGGAGG CTTGTTTGGA 
GCTGGAAGTG GTGGGAGAAG TGGAGGCTTC TTCAGTGGTT TGGGAGGGAA GCCAAGTCAG 
GATGCGGCCA ATAAGAATCC CTTCAGTTCC TCCAGTGGAG GATTTGGATC TGCAGCTTCC 
CAGAATACTT CTAGCTTGTT TGGAAACAGC GGAGCAAAGA CCTTTGGATT TGGCAGCTCA 
TCTTTTGGAG AGCAGAAGCC TACAGGCACT TTCAGCTCTG GTGGTGGAAG TGTGGCATCT 
CAAGGCTTTG GATTCTCCAG CCCAACCAAA GCAGGTGGCT TCGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTCGG GGGATCCCCT GGGTTCGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCAACCT TTTCAAGCCC TCTGGGCTCA ACGGGAGGCA AAGTGTTCGG CGAGGGTACA 
GCAGCAGCCA GCGCTGGAGG ATTTGGGTTT GGTGGTACCA GTAACAATAC AGCATCCTTT 
GGCACGCTAG CAAATCAAAA CACCCCGACG TTTGGATCAC TGTCACAACA GGGTACCGGT 
TTTGGCACAC AAAGCAGTGG ATTCTCAGCT TTTGGGACAG GTGGAGGAGG GTTTGGTTTT 
GGATCATCTA ACACATCTTC CTCTGGATTT AGTGGCTGGA GAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014798500.1
CDS	78..6365
Translation	MGDDMYVIPEREMKDFQFRALKKVRIFDAPEDLPKERSNLLAVSNKYGLVFAGGGTSLQIFHTRNLLMQNQPGEDPNKIVENVQSTSVPMKFPVQHLALSCDNLTLSVCMMSSEIGSFIGFYDVRTLLNQAKQQKRAFAYHKLAKDSGSAATVNDLKWNPTSPTMVAICLSDGSISVLQVTDSVKVYATLPSSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPSLQEKKVIPCPPFYYETDNPVKVLDVLWIGTYVFTIVYAAADGTLETPPEVVIAILPKKEEKRPEKFVNFIEPCYGSCTERQHHYFLNYVEEWDLVLAASAASTEVSILARQGDQNWELWVLEDSSRAELPVTDKSDDTLPVGVAVDYTSNMPIPISDEKTLAPAPVLILLSTDGVLCPFYMINQNPGVRSLIVTPEPLPLGGERVPKSAGSSATTPTTPPTSSKPDTGPGVAAAAAPASSQPPAGSGAAAFSFMPSAVKMPGSTGSDPPPYPFTTDASKQSLAPSLTAAATFSFSSASSKASVTPAPSVGTTTATTTSFSFASMNFKPAAESSPGPPLSTTPVAQKSSFASAASSVKVNLNEKFSAVDTSSPTSSSGQSTSTMFSFSTTSKTASGGPLSQSMPLPASPPTPLKASTPVPAAVARPAQSMPSASLLQKTAKISPSAAKPSSAQGKSALPSAPALEKQAHQWKDSDPVIAGIREEIAHFQKELEELKARTSKACFQVGTPEEMKMLRTESDDLHTFLLEIKETTESLHGDISILKTTLLEGFAGVEEAREQNERNHSSGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKRKQKRLIVPERETLFNTLANNREIINQQRKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSLSSAQSFDSDLESLRNALMKTTLESHAKPLPKVPAKLSPVKQAQLRNFLAKRKTPPIRSIAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENDDSQAVDQEEEAAPVQIPRHAPVVRTPSIQPGLMGQSPSFGKQPLTVGSSLPSSSSRIIPQGADSTMLATKTVKHGAPAPSVTVPAPQAAAAAALRRQMANQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSAVIGSSVPHSAAQAVHQVLATVATNQAKQTPLTSSLKGQTPPSSVCAPCPMASTSGPTSSGNKISGQGTVKTVTAVTSSAVTSVQTTSAQLNKAFSFSTVGSGFNFGAVTSAASSPTLSSLASSQAPAKESTQTESFSLAGGSKFAVVPESNFTFGSLKPASAPGTLVGNSSSADGVQSNKPAATTSSAPSTTSGKLDIPPSKPDHLFQSNLAGETLGSFSGLRVGQADDNAKTTTKAPSTSVAGAQSTKVSTIPAGFNFGAPLSLGKAGEAASTSATSTSMAPPSISTSATGSLFGNVQITSTGSSGVFNLGGSSGNKPTTFSFGVQQANSMSTSTSTPSTLSASPSLSFGNFLSSSQTAVPAATSSKVAGDAKLPPVSEKPSENEAVAAGSSPPMTQSPQTQVQLPESVKEPTLPQATAGDTTSAGPVATSAAPAPDAIAAPASTSDVKAPVPPAVSASTPPDQNVSATTSAANIASPAEATSVLAATSDVTTSVQSPAGGASVFAPTSAGSSVFAQPPSSSSSGSAFSQPAGDTTATPSTPPVFGQLPAGTTASSPFGQLTTSTLSTATATTQAAASSGFGTSAFGTTTTGGFGQPTFGQAPTFGQPASSSSGGFTFSQSAFGTMPAFGQPTASTAASSSGNVFGASASTNSASSFSFGQPSASTGGGLFGQTSPPVFGQSTGFGQTASVFGSAAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGSTVFGQQPSSSSGGLFGAGSGGRSGGFFSGLGGKPSQDAANKNPFSSSSGGFGSAASQNTSSLFGNSGAKTFGFGSSSFGEQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFGTLANQNTPTFGSLSQQGTGFGTQSSGFSAFGTGGGGFGFGSSNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_014943014.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014943014.1

ORF Insert Sequence:

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCAG CCATCTTCTT CCAGTGGAGG CTTGTTTGGA 
GCTGGAAGTG GTGGGAGAAG TGGAGGCTTC TTCAGTGGTT TGGGAGGGAA GCCAAGTCAG 
GATGCGGCCA ATAAGAATCC CTTCAGTTCC TCCAGTGGAG GATTTGGATC TGCAGCTTCC 
CAGAATACTT CTAGCTTGTT TGGAAACAGC GGAGCAAAGA CCTTTGGATT TGGCAGCTCA 
TCTTTTGGAG AGCAGAAGCC TACAGGCACT TTCAGCTCTG GTGGTGGAAG TGTGGCATCT 
CAAGGCTTTG GATTCTCCAG CCCAACCAAA GCAGGTGGCT TCGGTGCTGC TCCAGTGTTT 
GGCAGCCCTC CTACTTTCGG GGGATCCCCT GGGTTCGGAG GGGTGCCAGC ATTCGGTTCA 
GCCCCAACCT TTTCAAGCCC TCTGGGCTCA ACGGGAGGCA AAGTGTTCGG CGAGGGTACA 
GCAGCAGCCA GCGCTGGAGG ATTTGGGTTT GGTGGTACCA GTAACAATAC AGCATCCTTT 
GGCACGCTAG CAAATCAAAA CACCCCGACG TTTGGATCAC TGTCACAACA GGGTACCGGT 
TTTGGCACAC AAAGCAGTGG ATTCTCAGCT TTTGGGACAG GTGGAGGAGG GTTTGGTTTT 
GGATCATCTA ACACATCTTC CTCTGGATTT AGTGGCTGGA GAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_014943016.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014798502.1 Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

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CloneID	OCa65774
Clone ID Related Accession (Same CDS sequence)	XM_014943016.1
Accession Version	XM_014943016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090846.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCCA TCTTCTTCCA GTGGAGGCTT GTTTGGAGCT 
GGAAGTGGTG GGAGAAGTGG AGGCTTCTTC AGTGGTTTGG GAGGGAAGCC AAGTCAGGAT 
GCGGCCAATA AGAATCCCTT CAGTTCCTCC AGTGGAGGAT TTGGATCTGC AGCTTCCCAG 
AATACTTCTA GCTTGTTTGG AAACAGCGGA GCAAAGACCT TTGGATTTGG CAGCTCATCT 
TTTGGAGAGC AGAAGCCTAC AGGCACTTTC AGCTCTGGTG GTGGAAGTGT GGCATCTCAA 
GGCTTTGGAT TCTCCAGCCC AACCAAAGCA GGTGGCTTCG GTGCTGCTCC AGTGTTTGGC 
AGCCCTCCTA CTTTCGGGGG ATCCCCTGGG TTCGGAGGGG TGCCAGCATT CGGTTCAGCC 
CCAACCTTTT CAAGCCCTCT GGGCTCAACG GGAGGCAAAG TGTTCGGCGA GGGTACAGCA 
GCAGCCAGCG CTGGAGGATT TGGGTTTGGT GGTACCAGTA ACAATACAGC ATCCTTTGGC 
ACGCTAGCAA ATCAAAACAC CCCGACGTTT GGATCACTGT CACAACAGGG TACCGGTTTT 
GGCACACAAA GCAGTGGATT CTCAGCTTTT GGGACAGGTG GAGGAGGGTT TGGTTTTGGA 
TCATCTAACA CATCTTCCTC TGGATTTAGT GGCTGGAGAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014798502.1
CDS	1..6285
Translation	MGDDMYVIPEREMKDFQFRALKKVRIFDAPEDLPKERSNLLAVSNKYGLVFAGGGTSLQIFHTRNLLMQNQPGEDPNKIVENVQSTSVPMKFPVQHLALSCDNLTLSVCMMSSEIGSFIGFYDVRTLLNQAKQQKRAFAYHKLAKDSGSAATVNDLKWNPTSPTMVAICLSDGSISVLQVTDSVKVYATLPSSVAVTSVCWSPKGKQLAVGRQNGTVVQYLPSLQEKKVIPCPPFYYETDNPVKVLDVLWIGTYVFTIVYAAADGTLETPPEVVIAILPKKEEKRPEKFVNFIEPCYGSCTERQHHYFLNYVEEWDLVLAASAASTEVSILARQGDQNWELWVLEDSSRAELPVTDKSDDTLPVGVAVDYTSNMPIPISDEKTLAPAPVLILLSTDGVLCPFYMINQNPGVRSLIVTPEPLPLGGERVPKSAGSSATTPTTPPTSSKPDTGPGVAAAAAPASSQPPAGSGAAAFSFMPSAVKMPGSTGSDPPPYPFTTDASKQSLAPSLTAAATFSFSSASSKASVTPAPSVGTTTATTTSFSFASMNFKPAAESSPGPPLSTTPVAQKSSFASAASSVKVNLNEKFSAVDTSSPTSSSGQSTSTMFSFSTTSKTASGGPLSQSMPLPASPPTPLKASTPVPAAVARPAQSMPSASLLQKTAKISPSAAKPSSAQGKSALPSAPALEKQAHQWKDSDPVIAGIREEIAHFQKELEELKARTSKACFQVGTPEEMKMLRTESDDLHTFLLEIKETTESLHGDISILKTTLLEGFAGVEEAREQNERNHSSGYLHLLYKKPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKRKQKRLIVPERETLFNTLANNREIINQQRKRLNQLVNSLQQLRLYNQTSQWSVPSDTSSLSSAQSFDSDLESLRNALMKTTLESHAKPLPKVPAKLSPVKQAQLRNFLAKRKTPPIRSIAPASLSRSAFLSTRYYEDLDEVSSTSSVSQSLENDDSQAVDQEEEAAPVQIPRHAPVVRTPSIQPGLMGQSPSFGKQPLTVGSSLPSSSSRIIPQGADSTMLATKTVKHGAPAPSVTVPAPQAAAAAALRRQMANQSPAVSTSLTESTLKNVPQVVNVRELKTGALTPAVSAVIGSSVPHSAAQAVHQVLATVATNQAKQTPLTSSLKGQTPPSSVCAPCPMASTSGPTSSGNKISGQGTVKTVTAVTSSAVTSVQTTSAQLNKAFSFSTVGSGFNFGAVTSAASSPTLSSLASSQAPAKESTQTESFSLAGGSKFAVVPESNFTFGSLKPASAPGTLVGNSSSADGVQSNKPAATTSSAPSTTSGKLDIPPSKPDHLFQSNLAGETLGSFSGLRVGQADDNAKTTTKAPSTSVAGAQSTKVSTIPAGFNFGAPLSLGKAGEAASTSATSTSMAPPSISTSATGSLFGNVQITSTGSSGVFNLGGSSGNKPTTFSFGVQQANSMSTSTSTPSTLSASPSLSFGNFLSSSQTAVPAATSSKVAGDAKLPPVSEKPSENEAVAAGSSPPMTQSPQTQVQLPESVKEPTLPQATAGDTTSAGPVATSAAPAPDAIAAPASTSDVKAPVPPAVSASTPPDQNVSATTSAANIASPAEATSVLAATSDVTTSVQSPAGGASVFAPTSAGSSVFAQPPSSSSSGSAFSQPAGDTTATPSTPPVFGQLPAGTTASSPFGQLTTSTLSTATATTQAAASSGFGTSAFGTTTTGGFGQPTFGQAPTFGQPASSSSGGFTFSQSAFGTMPAFGQPTASTAASSSGNVFGASASTNSASSFSFGQPSASTGGGLFGQTSPPVFGQSTGFGQTASVFGSAAAVTTTTSSAGFSFCQASGFGSSNTGSVFGQAANTGSTVFGQPSSSSGGLFGAGSGGRSGGFFSGLGGKPSQDAANKNPFSSSSGGFGSAASQNTSSLFGNSGAKTFGFGSSSFGEQKPTGTFSSGGGSVASQGFGFSSPTKAGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPTFSSPLGSTGGKVFGEGTAAASAGGFGFGGTSNNTASFGTLANQNTPTFGSLSQQGTGFGTQSSGFSAFGTGGGGFGFGSSNTSSSGFSGWRS

Target ORF information:

RefSeq Version	XM_014943016.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax nucleoporin 214kDa (NUP214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014943016.1

ORF Insert Sequence:

ATGGGGGACG ATATGTACGT GATCCCGGAG CGGGAGATGA AGGACTTTCA GTTCAGAGCA 
CTGAAGAAGG TCCGCATCTT TGATGCTCCA GAGGATCTTC CCAAAGAGCG CTCCAATCTC 
CTTGCGGTCT CTAACAAATA CGGGCTCGTC TTTGCTGGTG GAGGAACCAG CCTGCAGATC 
TTCCACACCA GAAATCTTCT CATGCAAAAC CAGCCGGGAG AAGATCCCAA TAAAATCGTG 
GAGAATGTTC AGAGTACTTC AGTCCCCATG AAGTTTCCTG TGCAACACCT GGCTCTGAGC 
TGTGATAATC TCACACTGTC CGTCTGTATG ATGTCCAGTG AGATCGGTTC CTTCATTGGC 
TTTTATGACG TCCGCACACT TTTAAATCAG GCAAAACAGC AGAAACGTGC ATTTGCTTAT 
CACAAACTGG CCAAGGACTC GGGCAGCGCA GCAACGGTCA ATGACCTGAA GTGGAACCCC 
ACTAGTCCCA CCATGGTGGC CATCTGCCTC TCCGATGGCA GCATCTCTGT CCTGCAGGTC 
ACAGACTCTG TGAAAGTGTA TGCCACACTG CCTTCCTCTG TCGCTGTCAC ATCTGTATGT 
TGGAGCCCGA AGGGAAAGCA ACTGGCAGTA GGGAGGCAAA ATGGCACCGT GGTGCAGTAT 
CTGCCCAGTC TGCAGGAGAA GAAAGTAATC CCTTGTCCTC CCTTTTATTA TGAGACTGAC 
AATCCGGTTA AAGTTTTGGA TGTGCTGTGG ATTGGCACAT ATGTCTTTAC TATTGTATAT 
GCAGCAGCTG ATGGGACGCT GGAAACACCT CCAGAAGTGG TCATAGCCAT ATTGCCTAAA 
AAGGAAGAGA AGCGACCAGA GAAGTTTGTG AACTTCATAG AGCCTTGCTA TGGGAGCTGC 
ACGGAGAGAC AGCATCATTA CTTCCTCAAC TATGTTGAGG AATGGGATCT AGTTCTGGCA 
GCTTCAGCAG CTTCCACAGA AGTCAGCATT CTTGCCAGGC AAGGGGATCA GAACTGGGAA 
TTGTGGGTTT TGGAGGATTC CAGCAGGGCA GAACTTCCTG TGACAGATAA AAGCGATGAC 
ACTCTGCCTG TTGGCGTTGC TGTGGACTAC ACCAGTAACA TGCCCATCCC TATCAGTGAT 
GAGAAGACCC TTGCTCCAGC TCCAGTTCTG ATCTTGCTCT CTACGGATGG TGTGCTCTGC 
CCGTTCTACA TGATCAACCA AAACCCGGGG GTCAGATCAC TTATCGTCAC CCCAGAGCCG 
TTGCCGTTAG GAGGGGAGAG AGTCCCAAAA TCAGCAGGAA GTTCTGCCAC AACTCCAACC 
ACTCCTCCAA CTTCTTCAAA GCCTGACACG GGACCAGGTG TTGCTGCTGC TGCTGCGCCT 
GCCTCAAGTC AGCCTCCTGC AGGGAGTGGA GCAGCTGCTT TTTCCTTCAT GCCTTCAGCT 
GTGAAGATGC CTGGCTCCAC GGGCAGTGAT CCACCACCTT ACCCCTTCAC CACTGATGCC 
TCCAAGCAGT CCCTGGCTCC CAGTCTGACT GCAGCAGCAA CGTTCTCTTT CTCCTCTGCT 
TCTTCTAAGG CATCGGTGAC TCCTGCTCCT TCTGTAGGCA CCACTACAGC TACTACCACC 
TCATTTTCAT TTGCATCCAT GAACTTTAAA CCAGCTGCAG AGAGCTCACC TGGGCCACCG 
CTCTCCACAA CGCCTGTTGC ACAGAAGTCA TCTTTTGCCT CTGCAGCTTC TTCAGTCAAA 
GTGAACCTCA ATGAGAAGTT CTCTGCTGTA GACACTTCTT CCCCCACTAG CAGCAGTGGT 
CAAAGCACTT CCACTATGTT TTCGTTCTCA ACCACTTCAA AAACTGCTTC TGGAGGTCCT 
CTGAGCCAGT CCATGCCCCT CCCAGCCTCT CCTCCCACAC CACTGAAGGC TTCGACCCCT 
GTGCCTGCCG CAGTGGCACG TCCTGCTCAA AGTATGCCTT CAGCTTCTTT ACTACAGAAG 
ACAGCCAAAA TCTCCCCATC TGCAGCAAAG CCAAGCTCTG CTCAGGGAAA GTCAGCACTC 
CCCAGTGCTC CAGCTTTAGA GAAACAGGCC CACCAGTGGA AGGATTCAGA CCCTGTCATA 
GCAGGAATCA GAGAGGAGAT TGCACATTTT CAAAAGGAGT TGGAGGAACT GAAGGCCAGG 
ACTTCCAAAG CCTGTTTCCA AGTAGGTACT CCAGAAGAAA TGAAGATGTT GCGAACTGAG 
TCAGATGATC TTCACACCTT CCTCTTGGAA ATTAAAGAGA CAACGGAGTC ACTTCACGGA 
GATATTAGTA TTCTGAAGAC AACCTTGCTG GAGGGATTTG CAGGGGTAGA AGAGGCAAGA 
GAACAGAATG AACGGAATCA TAGCTCGGGG TATTTGCATT TGCTCTATAA GAAACCTTTG 
GATCCCAAGA GTGAAGCACA GCTTCAGGAA ATTCGACGCT TGCACCAGTA CGTGAAGTTT 
GCTGTTCAGG ATGTGAATGA TGTCCTGGAT TTGGAGTGGG ATCGGCACCT GGAACAGAAG 
AGAAAGCAGA AGCGTCTGAT AGTTCCTGAG CGAGAGACTT TGTTTAACAC CCTGGCTAAT 
AACCGGGAAA TAATCAATCA GCAGAGGAAG AGACTGAACC AGCTCGTGAA CAGCCTGCAG 
CAGCTCCGAC TGTATAATCA GACATCTCAG TGGAGTGTTC CCAGTGATAC GTCTTCTCTC 
AGCAGTGCTC AAAGTTTTGA CAGTGACCTT GAAAGCCTGC GCAATGCCTT GATGAAAACT 
ACCTTGGAAT CACATGCCAA ACCTCTGCCC AAAGTACCTG CTAAATTATC CCCTGTGAAA 
CAGGCACAGT TGAGGAATTT TCTAGCCAAG AGGAAAACTC CACCAATTAG GTCTATAGCT 
CCAGCAAGTT TGTCCCGATC TGCCTTCTTG TCCACAAGAT ACTATGAAGA TTTGGATGAA 
GTCAGTTCAA CATCCTCCGT ATCCCAGTCG CTGGAAAATG ACGATTCACA GGCAGTGGAC 
CAGGAAGAAG AGGCTGCTCC AGTCCAAATC CCTCGTCACG CTCCTGTGGT CCGTACTCCC 
TCCATCCAGC CTGGGTTGAT GGGTCAGTCT CCATCCTTTG GGAAACAGCC CCTCACCGTG 
GGTTCTTCAC TGCCTTCATC TTCAAGCAGA ATCATACCAC AAGGTGCTGA CAGTACAATG 
CTTGCAACAA AGACTGTGAA ACATGGGGCC CCGGCTCCTT CTGTCACTGT TCCAGCACCA 
CAAGCAGCTG CTGCTGCAGC TCTGCGGAGG CAGATGGCTA ACCAGTCCCC AGCTGTAAGC 
ACCTCCTTGA CTGAATCAAC TCTGAAGAAC GTTCCTCAGG TGGTGAACGT GCGAGAACTC 
AAAACTGGTG CCTTGACTCC AGCTGTCTCC GCAGTCATAG GATCATCTGT ACCTCACTCT 
GCAGCCCAGG CAGTCCACCA GGTTTTGGCA ACTGTTGCTA CCAATCAAGC TAAACAGACA 
CCGCTAACGA GCTCGCTTAA GGGACAGACT CCACCTTCCT CTGTGTGTGC ACCATGTCCT 
ATGGCCTCTA CGTCTGGTCC AACGTCGTCT GGCAACAAAA TCTCAGGCCA AGGGACAGTG 
AAAACGGTTA CAGCTGTGAC TTCTTCTGCC GTGACATCAG TACAAACAAC AAGTGCCCAG 
CTTAACAAAG CGTTCTCCTT TTCTACTGTG GGGTCTGGAT TTAATTTTGG TGCTGTCACA 
TCAGCTGCTT CCTCACCGAC ACTGTCCAGC CTTGCTTCCT CACAAGCCCC TGCTAAAGAG 
TCCACCCAGA CTGAGTCATT TTCGCTTGCT GGGGGTTCCA AATTTGCAGT AGTCCCTGAA 
AGCAACTTTA CTTTTGGGTC CCTCAAGCCA GCAAGTGCTC CAGGAACCTT AGTAGGAAAT 
AGCTCCTCAG CAGATGGCGT GCAGTCGAAC AAACCTGCTG CAACAACTTC TTCTGCACCC 
TCCACAACCT CTGGCAAGCT GGATATCCCT CCATCTAAAC CAGATCACTT GTTTCAGAGC 
AACCTAGCTG GGGAAACTCT TGGGAGCTTC TCAGGACTAC GGGTTGGTCA AGCAGATGAT 
AATGCCAAGA CCACCACTAA AGCACCATCG ACCAGTGTTG CAGGAGCACA GTCAACCAAA 
GTATCTACAA TACCTGCTGG GTTTAATTTT GGTGCTCCCC TGTCGCTAGG AAAAGCTGGA 
GAAGCTGCTT CTACGTCAGC CACGTCAACT AGTATGGCAC CCCCATCCAT CAGCACTAGT 
GCCACAGGCA GTCTCTTTGG CAACGTCCAG ATAACCAGCA CAGGGTCTTC TGGGGTATTT 
AATCTTGGAG GCTCTAGTGG CAACAAACCC ACTACTTTTT CATTTGGCGT TCAGCAAGCA 
AACAGTATGA GCACGTCCAC CTCTACCCCT TCTACTCTCT CTGCTTCACC GTCCCTCTCA 
TTTGGAAATT TCTTGAGTTC CTCACAAACT GCTGTCCCTG CTGCAACTTC TAGCAAGGTT 
GCAGGGGATG CCAAATTACC ACCTGTGTCA GAAAAGCCAT CTGAAAATGA AGCTGTAGCA 
GCTGGGTCTT CACCTCCAAT GACGCAGTCA CCACAGACAC AGGTACAGCT TCCAGAGTCT 
GTTAAAGAAC CCACTTTACC CCAGGCCACA GCTGGAGACA CCACCTCTGC AGGCCCAGTT 
GCCACATCGG CAGCACCTGC CCCTGATGCC ATTGCTGCTC CTGCTTCCAC AAGTGATGTG 
AAGGCACCAG TGCCTCCTGC TGTCTCTGCA TCCACCCCAC CAGATCAGAA TGTCTCAGCA 
ACAACCTCTG CAGCGAACAT TGCCTCTCCT GCAGAAGCCA CCAGTGTGTT GGCTGCTACC 
TCTGATGTTA CTACCAGTGT TCAGAGTCCA GCTGGTGGTG CCTCTGTGTT TGCCCCCACT 
TCTGCAGGCT CCTCAGTGTT CGCTCAGCCT CCGAGTTCCA GCTCTTCTGG CTCAGCATTT 
AGCCAGCCTG CTGGTGACAC AACTGCCACT CCTTCCACGC CACCCGTTTT TGGACAACTA 
CCAGCAGGTA CCACAGCATC CTCTCCCTTT GGGCAACTCA CCACCAGTAC CTTGTCCACT 
GCCACCGCTA CCACGCAGGC TGCTGCCAGC TCGGGTTTTG GTACTTCGGC TTTCGGCACC 
ACCACCACTG GGGGCTTTGG TCAACCAACC TTTGGACAAG CACCAACCTT TGGGCAACCA 
GCCAGCAGTT CTTCAGGTGG CTTTACCTTT AGCCAGTCTG CGTTTGGGAC AATGCCAGCC 
TTTGGCCAGC CAACAGCTTC CACAGCGGCC TCCAGCAGCG GCAACGTTTT TGGAGCCTCG 
GCCAGCACCA ACAGTGCCAG CTCCTTTTCC TTCGGACAGC CATCTGCCAG CACTGGGGGT 
GGGTTGTTTG GACAAACCAG CCCTCCGGTG TTTGGGCAGA GCACTGGCTT TGGGCAAACG 
GCATCTGTCT TTGGGAGCGC TGCTGCCGTG ACCACGACGA CGTCCTCTGC TGGGTTCAGC 
TTCTGCCAAG CTTCAGGTTT TGGTTCTAGT AACACGGGTT CTGTGTTTGG GCAAGCAGCT 
AATACTGGAA GCACTGTCTT TGGCCAGCCA TCTTCTTCCA GTGGAGGCTT GTTTGGAGCT 
GGAAGTGGTG GGAGAAGTGG AGGCTTCTTC AGTGGTTTGG GAGGGAAGCC AAGTCAGGAT 
GCGGCCAATA AGAATCCCTT CAGTTCCTCC AGTGGAGGAT TTGGATCTGC AGCTTCCCAG 
AATACTTCTA GCTTGTTTGG AAACAGCGGA GCAAAGACCT TTGGATTTGG CAGCTCATCT 
TTTGGAGAGC AGAAGCCTAC AGGCACTTTC AGCTCTGGTG GTGGAAGTGT GGCATCTCAA 
GGCTTTGGAT TCTCCAGCCC AACCAAAGCA GGTGGCTTCG GTGCTGCTCC AGTGTTTGGC 
AGCCCTCCTA CTTTCGGGGG ATCCCCTGGG TTCGGAGGGG TGCCAGCATT CGGTTCAGCC 
CCAACCTTTT CAAGCCCTCT GGGCTCAACG GGAGGCAAAG TGTTCGGCGA GGGTACAGCA 
GCAGCCAGCG CTGGAGGATT TGGGTTTGGT GGTACCAGTA ACAATACAGC ATCCTTTGGC 
ACGCTAGCAA ATCAAAACAC CCCGACGTTT GGATCACTGT CACAACAGGG TACCGGTTTT 
GGCACACAAA GCAGTGGATT CTCAGCTTTT GGGACAGGTG GAGGAGGGTT TGGTTTTGGA 
TCATCTAACA CATCTTCCTC TGGATTTAGT GGCTGGAGAA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NUP214 cDNA ORF clone, Calidris pugnax(ruff)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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