MYO5A cDNA ORF clone, Calidris pugnax(ruff)

The following MYO5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCa66944 XM_014944628.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66945 XM_014944629.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66946 XM_014944630.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66947 XM_014944631.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66948 XM_014944632.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66949 XM_014944633.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCa66950 XM_014944634.1
Latest version!
Calidris pugnax myosin VA (MYO5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa66944
    Clone ID Related Accession (Same CDS sequence) XM_014944628.1
    Accession Version XM_014944628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5493bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGCAGG AACCAAGTGA GAAAAAGGCA CCATTGGATA TGTCTCTGTT CCTCAAGCTG
    CAGAAACGGG TTACGGAGCT GGAGCAAGAA AAGCAGTCCC TGCAGGATGA ACTGGACAGA
    AAGGAAGAAC AAGCTCTGCG TGCTAAAGCT AAGGAGGAAG AAAGGCCTCC AATGAGAGGT
    GCTGAGTTGG AGTACGAGTC ACTCAAGCGC CAAGAACTTG AATCTGAGAA TAAAAAGCTG
    AAGAATGAGT TGAATGAGCT GCAGAAGGCC CTCACAGAAA CACGATCTCC AGAGGTAACT
    GCTCCTGGTG CCCCTGCGTA CAGAGTCCTC CTGGATCAGC TGACGTCAGT CAGTGAAGAG
    CTCGAAGTAC GCAAGGAAGA AGTCCTCATC CTGAGGTCTC AGCTGGTTAG CCAGAAAGAG
    GCTATTCAAC CCAAGGAGGA TAAGAATACC ATGACAGATG CTACAATCCT CTTGGAGGAT
    GTACAAAAGA TGAAAGACAA AGGAGAAATA GCACAGGCGT ATATTGGACT GAAGGAAACA
    AACAGGCTGC TGGAGTCTCA GCTTCAGTCA CAGAAGAAGA GCCACGAGAA CGAGCTGGAA
    TCGCTGCGAG GTGAGATCCA AGGTCTGAAG GAAGAAAACA ATCGCCAGCA GCAGCTCCTG
    GCACAGAACC TGCAGCTGCC TCCAGAGGCC CGCATCGAAG CCAGTCTCCA GCATGAGATC
    ACCCGGCTGA CGAATGAGAA CTTGGATTTA ATGGAGCAGC TTGAAAAGCA AGACAAAACT
    GTTCGCAAGT TAAAGAAACA GTTGAAAGTA TTTGCTAAGA AGATCGGTGA ACTTGAAGTG
    GGCCAGATGG AGAACATATC ACCTGGACAA ATCATTGATG AACCTATTCG TCCAGTTAAC
    ATTCCACGGA AAGAAAAAGA CTTCCAAGGG ATGTTAGAAT ATAAGAAGGA GGATGAACAA
    AAACTTGTGA AGAATCTTAT TTTAGAGCTG AAACCACGTG GAGTAGCTGT CAACCTGATT
    CCAGGACTAC CAGCATATAT TTTGTTCATG TGTGTACGTC ATGCGGATTA CCTTAATGAT
    GACCAAAAAG TGCGGTCGTT GTTGACTTCC ACTATCAACG GCATCAAAAA AGTGTTGAAG
    AAAAGAGGTG ATGACTTTGA AACAGTGTCC TTCTGGCTCT CTAACACCTG CCGATTTTTG
    CACTGTTTGA AGCAATACAG TGGAGAAGAG GGTTTTATGA AGCATAATAC ACCTCGTCAA
    AATGAACACT GCCTCACTAA TTTTGATTTA GCTGAATACA GACAAGTGCT GAGCGACTTG
    GCTATTCAGA TTTACCAACA ACTTGTCCGA GTGCTGGAAA ACATTCTGCA ACCCATGATT
    GTTTCAGGAA TGCTGGAGCA TGAGACAATC CAAGGTGTCT CGGGGGTGAA ACCAACAGGG
    CTGCGGAAGA GAACATCCAG CATTGCTGAT GAAGGAACCT ACACTTTGGA CTCCATTATT
    CGGCAGCTGA ACTCTTTCCA TTCAGTGATG TGTCAGCACG GAATGGATCC AGAGCTGATC
    AAACAGGTTG TCAAGCAGAT GTTCTACATC ATTGGGGCTG TAACGCTTAA CAATCTTCTC
    CTGCGCAAGG ACATGTGCTC GTGGAGCAAA GGAATGCAGA TAAGATACAA TGTGAGTCAA
    CTGGAAGAAT GGCTACGTGA TAAAAATCTG ATGAATAGTG GGGCTAAAGA AACACTGGAA
    CCCCTCATCC AGGCTGCACA GTTGTTGCAA GTGAAAAAGA AAACAGATGA AGATGCAGAA
    GCCATTTGTT CGATGTGCAA TGCACTGACT ACAGCCCAGA TTGTAAAAGT TCTGAATTTG
    TATACTCCAG TTAATGAGTT TGAAGAGAGA GTCTTGGTAT CGTTTATACG TACAATACAG
    ATGCGTCTGA GAGACAGGAA GGACTCTCCT CAGTTGCTCA TGGATGCTAA ACATATCTTT
    CCTGTTACTT TTCCGTTTAA TCCATCCTCT CTGGCATTAG AAACCATTCA GATCCCAGCC
    AGCTTGGGCC TGGGCTTCAT ATCACGTGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800114.1
    CDS151..5643
    Translation

    Target ORF information:

    RefSeq Version XM_014944628.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGCAGG AACCAAGTGA GAAAAAGGCA CCATTGGATA TGTCTCTGTT CCTCAAGCTG
    CAGAAACGGG TTACGGAGCT GGAGCAAGAA AAGCAGTCCC TGCAGGATGA ACTGGACAGA
    AAGGAAGAAC AAGCTCTGCG TGCTAAAGCT AAGGAGGAAG AAAGGCCTCC AATGAGAGGT
    GCTGAGTTGG AGTACGAGTC ACTCAAGCGC CAAGAACTTG AATCTGAGAA TAAAAAGCTG
    AAGAATGAGT TGAATGAGCT GCAGAAGGCC CTCACAGAAA CACGATCTCC AGAGGTAACT
    GCTCCTGGTG CCCCTGCGTA CAGAGTCCTC CTGGATCAGC TGACGTCAGT CAGTGAAGAG
    CTCGAAGTAC GCAAGGAAGA AGTCCTCATC CTGAGGTCTC AGCTGGTTAG CCAGAAAGAG
    GCTATTCAAC CCAAGGAGGA TAAGAATACC ATGACAGATG CTACAATCCT CTTGGAGGAT
    GTACAAAAGA TGAAAGACAA AGGAGAAATA GCACAGGCGT ATATTGGACT GAAGGAAACA
    AACAGGCTGC TGGAGTCTCA GCTTCAGTCA CAGAAGAAGA GCCACGAGAA CGAGCTGGAA
    TCGCTGCGAG GTGAGATCCA AGGTCTGAAG GAAGAAAACA ATCGCCAGCA GCAGCTCCTG
    GCACAGAACC TGCAGCTGCC TCCAGAGGCC CGCATCGAAG CCAGTCTCCA GCATGAGATC
    ACCCGGCTGA CGAATGAGAA CTTGGATTTA ATGGAGCAGC TTGAAAAGCA AGACAAAACT
    GTTCGCAAGT TAAAGAAACA GTTGAAAGTA TTTGCTAAGA AGATCGGTGA ACTTGAAGTG
    GGCCAGATGG AGAACATATC ACCTGGACAA ATCATTGATG AACCTATTCG TCCAGTTAAC
    ATTCCACGGA AAGAAAAAGA CTTCCAAGGG ATGTTAGAAT ATAAGAAGGA GGATGAACAA
    AAACTTGTGA AGAATCTTAT TTTAGAGCTG AAACCACGTG GAGTAGCTGT CAACCTGATT
    CCAGGACTAC CAGCATATAT TTTGTTCATG TGTGTACGTC ATGCGGATTA CCTTAATGAT
    GACCAAAAAG TGCGGTCGTT GTTGACTTCC ACTATCAACG GCATCAAAAA AGTGTTGAAG
    AAAAGAGGTG ATGACTTTGA AACAGTGTCC TTCTGGCTCT CTAACACCTG CCGATTTTTG
    CACTGTTTGA AGCAATACAG TGGAGAAGAG GGTTTTATGA AGCATAATAC ACCTCGTCAA
    AATGAACACT GCCTCACTAA TTTTGATTTA GCTGAATACA GACAAGTGCT GAGCGACTTG
    GCTATTCAGA TTTACCAACA ACTTGTCCGA GTGCTGGAAA ACATTCTGCA ACCCATGATT
    GTTTCAGGAA TGCTGGAGCA TGAGACAATC CAAGGTGTCT CGGGGGTGAA ACCAACAGGG
    CTGCGGAAGA GAACATCCAG CATTGCTGAT GAAGGAACCT ACACTTTGGA CTCCATTATT
    CGGCAGCTGA ACTCTTTCCA TTCAGTGATG TGTCAGCACG GAATGGATCC AGAGCTGATC
    AAACAGGTTG TCAAGCAGAT GTTCTACATC ATTGGGGCTG TAACGCTTAA CAATCTTCTC
    CTGCGCAAGG ACATGTGCTC GTGGAGCAAA GGAATGCAGA TAAGATACAA TGTGAGTCAA
    CTGGAAGAAT GGCTACGTGA TAAAAATCTG ATGAATAGTG GGGCTAAAGA AACACTGGAA
    CCCCTCATCC AGGCTGCACA GTTGTTGCAA GTGAAAAAGA AAACAGATGA AGATGCAGAA
    GCCATTTGTT CGATGTGCAA TGCACTGACT ACAGCCCAGA TTGTAAAAGT TCTGAATTTG
    TATACTCCAG TTAATGAGTT TGAAGAGAGA GTCTTGGTAT CGTTTATACG TACAATACAG
    ATGCGTCTGA GAGACAGGAA GGACTCTCCT CAGTTGCTCA TGGATGCTAA ACATATCTTT
    CCTGTTACTT TTCCGTTTAA TCCATCCTCT CTGGCATTAG AAACCATTCA GATCCCAGCC
    AGCTTGGGCC TGGGCTTCAT ATCACGTGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66945
    Clone ID Related Accession (Same CDS sequence) XM_014944629.1
    Accession Version XM_014944629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGGAAC CAAGTGAGAA AAAGGCACCA TTGGATATGT CTCTGTTCCT CAAGCTGCAG
    AAACGGGTTA CGGAGCTGGA GCAAGAAAAG CAGTCCCTGC AGGATGAACT GGACAGAAAG
    GAAGAACAAG CTCTGCGTGC TAAAGCTAAG GAGGAAGAAA GGCCTCCAAT GAGAGGTGCT
    GAGTTGGAGT ACGAGTCACT CAAGCGCCAA GAACTTGAAT CTGAGAATAA AAAGCTGAAG
    AATGAGTTGA ATGAGCTGCA GAAGGCCCTC ACAGAAACAC GATCTCCAGA GGTAACTGCT
    CCTGGTGCCC CTGCGTACAG AGTCCTCCTG GATCAGCTGA CGTCAGTCAG TGAAGAGCTC
    GAAGTACGCA AGGAAGAAGT CCTCATCCTG AGGTCTCAGC TGGTTAGCCA GAAAGAGGCT
    ATTCAACCCA AGGAGGATAA GAATACCATG ACAGATGCTA CAATCCTCTT GGAGGATGTA
    CAAAAGATGA AAGACAAAGG AGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACAAAC
    AGGCTGCTGG AGTCTCAGCT TCAGTCACAG AAGAAGAGCC ACGAGAACGA GCTGGAATCG
    CTGCGAGGTG AGATCCAAGG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCCTGGCA
    CAGAACCTGC AGCTGCCTCC AGAGGCCCGC ATCGAAGCCA GTCTCCAGCA TGAGATCACC
    CGGCTGACGA ATGAGAACTT GGATTTAATG GAGCAGCTTG AAAAGCAAGA CAAAACTGTT
    CGCAAGTTAA AGAAACAGTT GAAAGTATTT GCTAAGAAGA TCGGTGAACT TGAAGTGGGC
    CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT
    CCACGGAAAG AAAAAGACTT CCAAGGGATG TTAGAATATA AGAAGGAGGA TGAACAAAAA
    CTTGTGAAGA ATCTTATTTT AGAGCTGAAA CCACGTGGAG TAGCTGTCAA CCTGATTCCA
    GGACTACCAG CATATATTTT GTTCATGTGT GTACGTCATG CGGATTACCT TAATGATGAC
    CAAAAAGTGC GGTCGTTGTT GACTTCCACT ATCAACGGCA TCAAAAAAGT GTTGAAGAAA
    AGAGGTGATG ACTTTGAAAC AGTGTCCTTC TGGCTCTCTA ACACCTGCCG ATTTTTGCAC
    TGTTTGAAGC AATACAGTGG AGAAGAGGGT TTTATGAAGC ATAATACACC TCGTCAAAAT
    GAACACTGCC TCACTAATTT TGATTTAGCT GAATACAGAC AAGTGCTGAG CGACTTGGCT
    ATTCAGATTT ACCAACAACT TGTCCGAGTG CTGGAAAACA TTCTGCAACC CATGATTGTT
    TCAGGAATGC TGGAGCATGA GACAATCCAA GGTGTCTCGG GGGTGAAACC AACAGGGCTG
    CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGG
    CAGCTGAACT CTTTCCATTC AGTGATGTGT CAGCACGGAA TGGATCCAGA GCTGATCAAA
    CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CGCTTAACAA TCTTCTCCTG
    CGCAAGGACA TGTGCTCGTG GAGCAAAGGA ATGCAGATAA GATACAATGT GAGTCAACTG
    GAAGAATGGC TACGTGATAA AAATCTGATG AATAGTGGGG CTAAAGAAAC ACTGGAACCC
    CTCATCCAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC
    ATTTGTTCGA TGTGCAATGC ACTGACTACA GCCCAGATTG TAAAAGTTCT GAATTTGTAT
    ACTCCAGTTA ATGAGTTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGATG
    CGTCTGAGAG ACAGGAAGGA CTCTCCTCAG TTGCTCATGG ATGCTAAACA TATCTTTCCT
    GTTACTTTTC CGTTTAATCC ATCCTCTCTG GCATTAGAAA CCATTCAGAT CCCAGCCAGC
    TTGGGCCTGG GCTTCATATC ACGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800115.1
    CDS151..5640
    Translation

    Target ORF information:

    RefSeq Version XM_014944629.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGGAAC CAAGTGAGAA AAAGGCACCA TTGGATATGT CTCTGTTCCT CAAGCTGCAG
    AAACGGGTTA CGGAGCTGGA GCAAGAAAAG CAGTCCCTGC AGGATGAACT GGACAGAAAG
    GAAGAACAAG CTCTGCGTGC TAAAGCTAAG GAGGAAGAAA GGCCTCCAAT GAGAGGTGCT
    GAGTTGGAGT ACGAGTCACT CAAGCGCCAA GAACTTGAAT CTGAGAATAA AAAGCTGAAG
    AATGAGTTGA ATGAGCTGCA GAAGGCCCTC ACAGAAACAC GATCTCCAGA GGTAACTGCT
    CCTGGTGCCC CTGCGTACAG AGTCCTCCTG GATCAGCTGA CGTCAGTCAG TGAAGAGCTC
    GAAGTACGCA AGGAAGAAGT CCTCATCCTG AGGTCTCAGC TGGTTAGCCA GAAAGAGGCT
    ATTCAACCCA AGGAGGATAA GAATACCATG ACAGATGCTA CAATCCTCTT GGAGGATGTA
    CAAAAGATGA AAGACAAAGG AGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACAAAC
    AGGCTGCTGG AGTCTCAGCT TCAGTCACAG AAGAAGAGCC ACGAGAACGA GCTGGAATCG
    CTGCGAGGTG AGATCCAAGG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCCTGGCA
    CAGAACCTGC AGCTGCCTCC AGAGGCCCGC ATCGAAGCCA GTCTCCAGCA TGAGATCACC
    CGGCTGACGA ATGAGAACTT GGATTTAATG GAGCAGCTTG AAAAGCAAGA CAAAACTGTT
    CGCAAGTTAA AGAAACAGTT GAAAGTATTT GCTAAGAAGA TCGGTGAACT TGAAGTGGGC
    CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT
    CCACGGAAAG AAAAAGACTT CCAAGGGATG TTAGAATATA AGAAGGAGGA TGAACAAAAA
    CTTGTGAAGA ATCTTATTTT AGAGCTGAAA CCACGTGGAG TAGCTGTCAA CCTGATTCCA
    GGACTACCAG CATATATTTT GTTCATGTGT GTACGTCATG CGGATTACCT TAATGATGAC
    CAAAAAGTGC GGTCGTTGTT GACTTCCACT ATCAACGGCA TCAAAAAAGT GTTGAAGAAA
    AGAGGTGATG ACTTTGAAAC AGTGTCCTTC TGGCTCTCTA ACACCTGCCG ATTTTTGCAC
    TGTTTGAAGC AATACAGTGG AGAAGAGGGT TTTATGAAGC ATAATACACC TCGTCAAAAT
    GAACACTGCC TCACTAATTT TGATTTAGCT GAATACAGAC AAGTGCTGAG CGACTTGGCT
    ATTCAGATTT ACCAACAACT TGTCCGAGTG CTGGAAAACA TTCTGCAACC CATGATTGTT
    TCAGGAATGC TGGAGCATGA GACAATCCAA GGTGTCTCGG GGGTGAAACC AACAGGGCTG
    CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGG
    CAGCTGAACT CTTTCCATTC AGTGATGTGT CAGCACGGAA TGGATCCAGA GCTGATCAAA
    CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CGCTTAACAA TCTTCTCCTG
    CGCAAGGACA TGTGCTCGTG GAGCAAAGGA ATGCAGATAA GATACAATGT GAGTCAACTG
    GAAGAATGGC TACGTGATAA AAATCTGATG AATAGTGGGG CTAAAGAAAC ACTGGAACCC
    CTCATCCAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC
    ATTTGTTCGA TGTGCAATGC ACTGACTACA GCCCAGATTG TAAAAGTTCT GAATTTGTAT
    ACTCCAGTTA ATGAGTTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGATG
    CGTCTGAGAG ACAGGAAGGA CTCTCCTCAG TTGCTCATGG ATGCTAAACA TATCTTTCCT
    GTTACTTTTC CGTTTAATCC ATCCTCTCTG GCATTAGAAA CCATTCAGAT CCCAGCCAGC
    TTGGGCCTGG GCTTCATATC ACGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66946
    Clone ID Related Accession (Same CDS sequence) XM_014944630.1
    Accession Version XM_014944630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGCAGGAAC CAAGTGAGAA AAAGGCACCA TTGGATATGT CTCTGTTCCT CAAGCTGCAG
    AAACGGGTTA CGGAGCTGGA GCAAGAAAAG CAGTCCCTGC AGGATGAACT GGACAGAAAG
    GAAGAACAAG CTCTGCGTGC TAAAGCTAAG GAGGAAGAAA GGCCTCCAAT GAGAGGTGCT
    GAGTTGGAGT ACGAGTCACT CAAGCGCCAA GAACTTGAAT CTGAGAATAA AAAGCTGAAG
    AATGAGTTGA ATGAGCTGCA GAAGGCCCTC ACAGAAACAC GATCTCCAGA GGTAACTGCT
    CCTGGTGCCC CTGCGTACAG AGTCCTCCTG GATCAGCTGA CGTCAGTCAG TGAAGAGCTC
    GAAGTACGCA AGGAAGAAGT CCTCATCCTG AGGTCTCAGC TGGTTAGCCA GAAAGAGGCT
    ATTCAACCCA AGGAGGATAA GAATACCATG ACAGATGCTA CAATCCTCTT GGAGGATGTA
    CAAAAGATGA AAGACAAAGG AGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACAAAC
    AGGCTGCTGG AGTCTCAGCT TCAGTCACAG AAGAAGAGCC ACGAGAACGA GCTGGAATCG
    CTGCGAGGTG AGATCCAAGG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCCTGGCA
    CAGAACCTGC AGCTGCCTCC AGAGGCCCGC ATCGAAGCCA GTCTCCAGCA TGAGATCACC
    CGGCTGACGA ATGAGAACTT GGATTTAATG GAGCAGCTTG AAAAGCAAGA CAAAACTGTT
    CGCAAGTTAA AGAAACAGTT GAAAGTATTT GCTAAGAAGA TCGGTGAACT TGAAGTGGGC
    CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT
    CCACGGAAAG AAAAAGACTT CCAAGGGATG TTAGAATATA AGAAGGAGGA TGAACAAAAA
    CTTGTGAAGA ATCTTATTTT AGAGCTGAAA CCACGTGGAG TAGCTGTCAA CCTGATTCCA
    GGACTACCAG CATATATTTT GTTCATGTGT GTACGTCATG CGGATTACCT TAATGATGAC
    CAAAAAGTGC GGTCGTTGTT GACTTCCACT ATCAACGGCA TCAAAAAAGT GTTGAAGAAA
    AGAGGTGATG ACTTTGAAAC AGTGTCCTTC TGGCTCTCTA ACACCTGCCG ATTTTTGCAC
    TGTTTGAAGC AATACAGTGG AGAAGAGGGT TTTATGAAGC ATAATACACC TCGTCAAAAT
    GAACACTGCC TCACTAATTT TGATTTAGCT GAATACAGAC AAGTGCTGAG CGACTTGGCT
    ATTCAGATTT ACCAACAACT TGTCCGAGTG CTGGAAAACA TTCTGCAACC CATGATTGTT
    TCAGGAATGC TGGAGCATGA GACAATCCAA GGTGTCTCGG GGGTGAAACC AACAGGGCTG
    CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGG
    CAGCTGAACT CTTTCCATTC AGTGATGTGT CAGCACGGAA TGGATCCAGA GCTGATCAAA
    CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CGCTTAACAA TCTTCTCCTG
    CGCAAGGACA TGTGCTCGTG GAGCAAAGGA ATGCAGATAA GATACAATGT GAGTCAACTG
    GAAGAATGGC TACGTGATAA AAATCTGATG AATAGTGGGG CTAAAGAAAC ACTGGAACCC
    CTCATCCAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC
    ATTTGTTCGA TGTGCAATGC ACTGACTACA GCCCAGATTG TAAAAGTTCT GAATTTGTAT
    ACTCCAGTTA ATGAGTTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGATG
    CGTCTGAGAG ACAGGAAGGA CTCTCCTCAG TTGCTCATGG ATGCTAAACA TATCTTTCCT
    GTTACTTTTC CGTTTAATCC ATCCTCTCTG GCATTAGAAA CCATTCAGAT CCCAGCCAGC
    TTGGGCCTGG GCTTCATATC ACGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800116.1
    CDS151..5640
    Translation

    Target ORF information:

    RefSeq Version XM_014944630.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGCAGGAAC CAAGTGAGAA AAAGGCACCA TTGGATATGT CTCTGTTCCT CAAGCTGCAG
    AAACGGGTTA CGGAGCTGGA GCAAGAAAAG CAGTCCCTGC AGGATGAACT GGACAGAAAG
    GAAGAACAAG CTCTGCGTGC TAAAGCTAAG GAGGAAGAAA GGCCTCCAAT GAGAGGTGCT
    GAGTTGGAGT ACGAGTCACT CAAGCGCCAA GAACTTGAAT CTGAGAATAA AAAGCTGAAG
    AATGAGTTGA ATGAGCTGCA GAAGGCCCTC ACAGAAACAC GATCTCCAGA GGTAACTGCT
    CCTGGTGCCC CTGCGTACAG AGTCCTCCTG GATCAGCTGA CGTCAGTCAG TGAAGAGCTC
    GAAGTACGCA AGGAAGAAGT CCTCATCCTG AGGTCTCAGC TGGTTAGCCA GAAAGAGGCT
    ATTCAACCCA AGGAGGATAA GAATACCATG ACAGATGCTA CAATCCTCTT GGAGGATGTA
    CAAAAGATGA AAGACAAAGG AGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACAAAC
    AGGCTGCTGG AGTCTCAGCT TCAGTCACAG AAGAAGAGCC ACGAGAACGA GCTGGAATCG
    CTGCGAGGTG AGATCCAAGG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCCTGGCA
    CAGAACCTGC AGCTGCCTCC AGAGGCCCGC ATCGAAGCCA GTCTCCAGCA TGAGATCACC
    CGGCTGACGA ATGAGAACTT GGATTTAATG GAGCAGCTTG AAAAGCAAGA CAAAACTGTT
    CGCAAGTTAA AGAAACAGTT GAAAGTATTT GCTAAGAAGA TCGGTGAACT TGAAGTGGGC
    CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT
    CCACGGAAAG AAAAAGACTT CCAAGGGATG TTAGAATATA AGAAGGAGGA TGAACAAAAA
    CTTGTGAAGA ATCTTATTTT AGAGCTGAAA CCACGTGGAG TAGCTGTCAA CCTGATTCCA
    GGACTACCAG CATATATTTT GTTCATGTGT GTACGTCATG CGGATTACCT TAATGATGAC
    CAAAAAGTGC GGTCGTTGTT GACTTCCACT ATCAACGGCA TCAAAAAAGT GTTGAAGAAA
    AGAGGTGATG ACTTTGAAAC AGTGTCCTTC TGGCTCTCTA ACACCTGCCG ATTTTTGCAC
    TGTTTGAAGC AATACAGTGG AGAAGAGGGT TTTATGAAGC ATAATACACC TCGTCAAAAT
    GAACACTGCC TCACTAATTT TGATTTAGCT GAATACAGAC AAGTGCTGAG CGACTTGGCT
    ATTCAGATTT ACCAACAACT TGTCCGAGTG CTGGAAAACA TTCTGCAACC CATGATTGTT
    TCAGGAATGC TGGAGCATGA GACAATCCAA GGTGTCTCGG GGGTGAAACC AACAGGGCTG
    CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGG
    CAGCTGAACT CTTTCCATTC AGTGATGTGT CAGCACGGAA TGGATCCAGA GCTGATCAAA
    CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CGCTTAACAA TCTTCTCCTG
    CGCAAGGACA TGTGCTCGTG GAGCAAAGGA ATGCAGATAA GATACAATGT GAGTCAACTG
    GAAGAATGGC TACGTGATAA AAATCTGATG AATAGTGGGG CTAAAGAAAC ACTGGAACCC
    CTCATCCAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC
    ATTTGTTCGA TGTGCAATGC ACTGACTACA GCCCAGATTG TAAAAGTTCT GAATTTGTAT
    ACTCCAGTTA ATGAGTTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGATG
    CGTCTGAGAG ACAGGAAGGA CTCTCCTCAG TTGCTCATGG ATGCTAAACA TATCTTTCCT
    GTTACTTTTC CGTTTAATCC ATCCTCTCTG GCATTAGAAA CCATTCAGAT CCCAGCCAGC
    TTGGGCCTGG GCTTCATATC ACGTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66947
    Clone ID Related Accession (Same CDS sequence) XM_014944631.1
    Accession Version XM_014944631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGG AGGATAAGAA TACCATGACA GATGCTACAA TCCTCTTGGA GGATGTACAA
    AAGATGAAAG ACAAAGGAGA AATAGCACAG GCGTATATTG GACTGAAGGA AACAAACAGG
    CTGCTGGAGT CTCAGCTTCA GTCACAGAAG AAGAGCCACG AGAACGAGCT GGAATCGCTG
    CGAGGTGAGA TCCAAGGTCT GAAGGAAGAA AACAATCGCC AGCAGCAGCT CCTGGCACAG
    AACCTGCAGC TGCCTCCAGA GGCCCGCATC GAAGCCAGTC TCCAGCATGA GATCACCCGG
    CTGACGAATG AGAACTTGGA TTTAATGGAG CAGCTTGAAA AGCAAGACAA AACTGTTCGC
    AAGTTAAAGA AACAGTTGAA AGTATTTGCT AAGAAGATCG GTGAACTTGA AGTGGGCCAG
    ATGGAGAACA TATCACCTGG ACAAATCATT GATGAACCTA TTCGTCCAGT TAACATTCCA
    CGGAAAGAAA AAGACTTCCA AGGGATGTTA GAATATAAGA AGGAGGATGA ACAAAAACTT
    GTGAAGAATC TTATTTTAGA GCTGAAACCA CGTGGAGTAG CTGTCAACCT GATTCCAGGA
    CTACCAGCAT ATATTTTGTT CATGTGTGTA CGTCATGCGG ATTACCTTAA TGATGACCAA
    AAAGTGCGGT CGTTGTTGAC TTCCACTATC AACGGCATCA AAAAAGTGTT GAAGAAAAGA
    GGTGATGACT TTGAAACAGT GTCCTTCTGG CTCTCTAACA CCTGCCGATT TTTGCACTGT
    TTGAAGCAAT ACAGTGGAGA AGAGGGTTTT ATGAAGCATA ATACACCTCG TCAAAATGAA
    CACTGCCTCA CTAATTTTGA TTTAGCTGAA TACAGACAAG TGCTGAGCGA CTTGGCTATT
    CAGATTTACC AACAACTTGT CCGAGTGCTG GAAAACATTC TGCAACCCAT GATTGTTTCA
    GGAATGCTGG AGCATGAGAC AATCCAAGGT GTCTCGGGGG TGAAACCAAC AGGGCTGCGG
    AAGAGAACAT CCAGCATTGC TGATGAAGGA ACCTACACTT TGGACTCCAT TATTCGGCAG
    CTGAACTCTT TCCATTCAGT GATGTGTCAG CACGGAATGG ATCCAGAGCT GATCAAACAG
    GTTGTCAAGC AGATGTTCTA CATCATTGGG GCTGTAACGC TTAACAATCT TCTCCTGCGC
    AAGGACATGT GCTCGTGGAG CAAAGGAATG CAGATAAGAT ACAATGTGAG TCAACTGGAA
    GAATGGCTAC GTGATAAAAA TCTGATGAAT AGTGGGGCTA AAGAAACACT GGAACCCCTC
    ATCCAGGCTG CACAGTTGTT GCAAGTGAAA AAGAAAACAG ATGAAGATGC AGAAGCCATT
    TGTTCGATGT GCAATGCACT GACTACAGCC CAGATTGTAA AAGTTCTGAA TTTGTATACT
    CCAGTTAATG AGTTTGAAGA GAGAGTCTTG GTATCGTTTA TACGTACAAT ACAGATGCGT
    CTGAGAGACA GGAAGGACTC TCCTCAGTTG CTCATGGATG CTAAACATAT CTTTCCTGTT
    ACTTTTCCGT TTAATCCATC CTCTCTGGCA TTAGAAACCA TTCAGATCCC AGCCAGCTTG
    GGCCTGGGCT TCATATCACG TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800117.1
    CDS151..5637
    Translation

    Target ORF information:

    RefSeq Version XM_014944631.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGG AGGATAAGAA TACCATGACA GATGCTACAA TCCTCTTGGA GGATGTACAA
    AAGATGAAAG ACAAAGGAGA AATAGCACAG GCGTATATTG GACTGAAGGA AACAAACAGG
    CTGCTGGAGT CTCAGCTTCA GTCACAGAAG AAGAGCCACG AGAACGAGCT GGAATCGCTG
    CGAGGTGAGA TCCAAGGTCT GAAGGAAGAA AACAATCGCC AGCAGCAGCT CCTGGCACAG
    AACCTGCAGC TGCCTCCAGA GGCCCGCATC GAAGCCAGTC TCCAGCATGA GATCACCCGG
    CTGACGAATG AGAACTTGGA TTTAATGGAG CAGCTTGAAA AGCAAGACAA AACTGTTCGC
    AAGTTAAAGA AACAGTTGAA AGTATTTGCT AAGAAGATCG GTGAACTTGA AGTGGGCCAG
    ATGGAGAACA TATCACCTGG ACAAATCATT GATGAACCTA TTCGTCCAGT TAACATTCCA
    CGGAAAGAAA AAGACTTCCA AGGGATGTTA GAATATAAGA AGGAGGATGA ACAAAAACTT
    GTGAAGAATC TTATTTTAGA GCTGAAACCA CGTGGAGTAG CTGTCAACCT GATTCCAGGA
    CTACCAGCAT ATATTTTGTT CATGTGTGTA CGTCATGCGG ATTACCTTAA TGATGACCAA
    AAAGTGCGGT CGTTGTTGAC TTCCACTATC AACGGCATCA AAAAAGTGTT GAAGAAAAGA
    GGTGATGACT TTGAAACAGT GTCCTTCTGG CTCTCTAACA CCTGCCGATT TTTGCACTGT
    TTGAAGCAAT ACAGTGGAGA AGAGGGTTTT ATGAAGCATA ATACACCTCG TCAAAATGAA
    CACTGCCTCA CTAATTTTGA TTTAGCTGAA TACAGACAAG TGCTGAGCGA CTTGGCTATT
    CAGATTTACC AACAACTTGT CCGAGTGCTG GAAAACATTC TGCAACCCAT GATTGTTTCA
    GGAATGCTGG AGCATGAGAC AATCCAAGGT GTCTCGGGGG TGAAACCAAC AGGGCTGCGG
    AAGAGAACAT CCAGCATTGC TGATGAAGGA ACCTACACTT TGGACTCCAT TATTCGGCAG
    CTGAACTCTT TCCATTCAGT GATGTGTCAG CACGGAATGG ATCCAGAGCT GATCAAACAG
    GTTGTCAAGC AGATGTTCTA CATCATTGGG GCTGTAACGC TTAACAATCT TCTCCTGCGC
    AAGGACATGT GCTCGTGGAG CAAAGGAATG CAGATAAGAT ACAATGTGAG TCAACTGGAA
    GAATGGCTAC GTGATAAAAA TCTGATGAAT AGTGGGGCTA AAGAAACACT GGAACCCCTC
    ATCCAGGCTG CACAGTTGTT GCAAGTGAAA AAGAAAACAG ATGAAGATGC AGAAGCCATT
    TGTTCGATGT GCAATGCACT GACTACAGCC CAGATTGTAA AAGTTCTGAA TTTGTATACT
    CCAGTTAATG AGTTTGAAGA GAGAGTCTTG GTATCGTTTA TACGTACAAT ACAGATGCGT
    CTGAGAGACA GGAAGGACTC TCCTCAGTTG CTCATGGATG CTAAACATAT CTTTCCTGTT
    ACTTTTCCGT TTAATCCATC CTCTCTGGCA TTAGAAACCA TTCAGATCCC AGCCAGCTTG
    GGCCTGGGCT TCATATCACG TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66948
    Clone ID Related Accession (Same CDS sequence) XM_014944632.1
    Accession Version XM_014944632.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGCAGG AACCAAGTGA GAAAAAGGCA CCATTGGATA TGTCTCTGTT CCTCAAGCTG
    CAGAAACGGG TTACGGAGCT GGAGCAAGAA AAGCAGTCCC TGCAGGATGA ACTGGACAGA
    AAGGAAGAAC AAGCTCTGCG TGCTAAAGCT AAGGAGGAAG AAAGGCCTCC AATGAGAGGT
    GCTGAGTTGG AGTACGAGTC ACTCAAGCGC CAAGAACTTG AATCTGAGAA TAAAAAGCTG
    AAGAATGAGT TGAATGAGCT GCAGAAGGCC CTCACAGAAA CACGATCTCC AGAGGTAACT
    GCTCCTGGTG CCCCTGCGTA CAGAGTCCTC CTGGATCAGC TGACGTCAGT CAGTGAAGAG
    CTCGAAGTAC GCAAGGAAGA AGTCCTCATC CTGAGGTCTC AGCTGGTTAG CCAGAAAGAG
    GCTATTCAAC CCAAGAATAC CATGACAGAT GCTACAATCC TCTTGGAGGA TGTACAAAAG
    ATGAAAGACA AAGGAGAAAT AGCACAGGCG TATATTGGAC TGAAGGAAAC AAACAGGCTG
    CTGGAGTCTC AGCTTCAGTC ACAGAAGAAG AGCCACGAGA ACGAGCTGGA ATCGCTGCGA
    GGTGAGATCC AAGGTCTGAA GGAAGAAAAC AATCGCCAGC AGCAGCTCCT GGCACAGAAC
    CTGCAGCTGC CTCCAGAGGC CCGCATCGAA GCCAGTCTCC AGCATGAGAT CACCCGGCTG
    ACGAATGAGA ACTTGGATTT AATGGAGCAG CTTGAAAAGC AAGACAAAAC TGTTCGCAAG
    TTAAAGAAAC AGTTGAAAGT ATTTGCTAAG AAGATCGGTG AACTTGAAGT GGGCCAGATG
    GAGAACATAT CACCTGGACA AATCATTGAT GAACCTATTC GTCCAGTTAA CATTCCACGG
    AAAGAAAAAG ACTTCCAAGG GATGTTAGAA TATAAGAAGG AGGATGAACA AAAACTTGTG
    AAGAATCTTA TTTTAGAGCT GAAACCACGT GGAGTAGCTG TCAACCTGAT TCCAGGACTA
    CCAGCATATA TTTTGTTCAT GTGTGTACGT CATGCGGATT ACCTTAATGA TGACCAAAAA
    GTGCGGTCGT TGTTGACTTC CACTATCAAC GGCATCAAAA AAGTGTTGAA GAAAAGAGGT
    GATGACTTTG AAACAGTGTC CTTCTGGCTC TCTAACACCT GCCGATTTTT GCACTGTTTG
    AAGCAATACA GTGGAGAAGA GGGTTTTATG AAGCATAATA CACCTCGTCA AAATGAACAC
    TGCCTCACTA ATTTTGATTT AGCTGAATAC AGACAAGTGC TGAGCGACTT GGCTATTCAG
    ATTTACCAAC AACTTGTCCG AGTGCTGGAA AACATTCTGC AACCCATGAT TGTTTCAGGA
    ATGCTGGAGC ATGAGACAAT CCAAGGTGTC TCGGGGGTGA AACCAACAGG GCTGCGGAAG
    AGAACATCCA GCATTGCTGA TGAAGGAACC TACACTTTGG ACTCCATTAT TCGGCAGCTG
    AACTCTTTCC ATTCAGTGAT GTGTCAGCAC GGAATGGATC CAGAGCTGAT CAAACAGGTT
    GTCAAGCAGA TGTTCTACAT CATTGGGGCT GTAACGCTTA ACAATCTTCT CCTGCGCAAG
    GACATGTGCT CGTGGAGCAA AGGAATGCAG ATAAGATACA ATGTGAGTCA ACTGGAAGAA
    TGGCTACGTG ATAAAAATCT GATGAATAGT GGGGCTAAAG AAACACTGGA ACCCCTCATC
    CAGGCTGCAC AGTTGTTGCA AGTGAAAAAG AAAACAGATG AAGATGCAGA AGCCATTTGT
    TCGATGTGCA ATGCACTGAC TACAGCCCAG ATTGTAAAAG TTCTGAATTT GTATACTCCA
    GTTAATGAGT TTGAAGAGAG AGTCTTGGTA TCGTTTATAC GTACAATACA GATGCGTCTG
    AGAGACAGGA AGGACTCTCC TCAGTTGCTC ATGGATGCTA AACATATCTT TCCTGTTACT
    TTTCCGTTTA ATCCATCCTC TCTGGCATTA GAAACCATTC AGATCCCAGC CAGCTTGGGC
    CTGGGCTTCA TATCACGTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800118.1
    CDS151..5634
    Translation

    Target ORF information:

    RefSeq Version XM_014944632.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGAAGTTTAA GCTGCTGCCA GAGTTATTCC AGGATGAGGA GAAGGTCCTT
    AGTCCCACGT CAGCCACCCC TTCAGGGCGC GTACCTTTGT CTCGAACAGC TGTAAAACCA
    GCCAAGGCCC GGCCAGGTCA AGCCAGCAAG GAGCATAAGA AAACTGTGGG ACATCAGTTT
    CGAAACTCTC TTCACCTGCT GATGGAAACC CTGAATGCTA CAACCCCACA CTACGTGCGC
    TGTATTAAGC CCAACGACTT CAAGTTTCCG TTCACATTCG ATGAAAAGCG GGCAGTGCAG
    CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG CTTCCCTTCC
    AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TGATGAAGCA GAGAGATGTC
    CTTAGTGACC GAAAGCAGAC ATGTAAAAAT GTCCTGGAAA AGCTAATTCT GGACAAGGAT
    AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTGGAA
    AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC
    TGGCTGATGC GAAAGAAGTA CGTGCGTATG AGGAAGGCTG CCATCACCAT TCAGAGACAT
    GTCAGAGGGT ACCAAGCACG ATGCTATGCC AAGTTTCTGC GGAGAACACG GGCTGCCATC
    ACTATTCAGA AGTTCCAGCG CATGTATGTG GTCCGCAAAA GATACCAATG CATGCGAGAT
    GCTACTATTG CTCTTCAGGC TCTCTTAAGA GGTTACATGG CCAGGAACAA ATACCAAATG
    ATGCTTCGGG AGCACAAGTC TATCATTATT CAGAAACATA TAAGAGGCTG GCTGGCCCGA
    GTGCACTACC GTAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG
    ATGGCCAAAA GGGAGCTGAA GAAACTGAAG ATAGAGGCCC GGTCTGTAGA ACGCTACAAG
    AAGCTTCACA TTGGCTTAGA GAACAAGATC ATGCAGCTGC AGCGAAAAAT TGATGAGCAG
    AACAAAGAGT ACAAATCTCT GCTGGAGAAG CTGAGCAACC TGGAGATCAC GTACAGTACA
    GAGACAGAGA AGCTGCGGAG TGACGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG
    AACGCGACCA ACCGTGTCCT CAGCCTGCAG GAGGAGATTG CCAAGCTCCG GAAGGAGCTG
    CACCAAACTC AGACTGAGAA GAAGACCATT GAGGAACGGG CAGACAAATA CAAACATGAA
    ACCGAACAGC TGGTTTCAGA GCTGAAAGAG CAGAACACGT TACTGAAAAC AGAGAAGGAG
    GAGCTGAACC GTCGCATCCA TGACCAGGCA AAGGAAATAA CAGAGACGAT GGAGAAGAAG
    CTAGTGGAGG AAACCAAGCA GCTAGAGCTA GATCTGAATG ATGAACGGTT ACGGTACCAG
    AACCTGCTGA ATGAGTTCAG CCGTTTAGAG GAGCGGTATG ATGATCTCAA GGATGAAATG
    AACTTAATGG TGAGCATCCC CAAGCCTGGG CACAAAAGAA CGGATTCAAC TCACAGTAGC
    AATGAATCTG AATACACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCAGTGAGG
    ATGGAGCAGG AACCAAGTGA GAAAAAGGCA CCATTGGATA TGTCTCTGTT CCTCAAGCTG
    CAGAAACGGG TTACGGAGCT GGAGCAAGAA AAGCAGTCCC TGCAGGATGA ACTGGACAGA
    AAGGAAGAAC AAGCTCTGCG TGCTAAAGCT AAGGAGGAAG AAAGGCCTCC AATGAGAGGT
    GCTGAGTTGG AGTACGAGTC ACTCAAGCGC CAAGAACTTG AATCTGAGAA TAAAAAGCTG
    AAGAATGAGT TGAATGAGCT GCAGAAGGCC CTCACAGAAA CACGATCTCC AGAGGTAACT
    GCTCCTGGTG CCCCTGCGTA CAGAGTCCTC CTGGATCAGC TGACGTCAGT CAGTGAAGAG
    CTCGAAGTAC GCAAGGAAGA AGTCCTCATC CTGAGGTCTC AGCTGGTTAG CCAGAAAGAG
    GCTATTCAAC CCAAGAATAC CATGACAGAT GCTACAATCC TCTTGGAGGA TGTACAAAAG
    ATGAAAGACA AAGGAGAAAT AGCACAGGCG TATATTGGAC TGAAGGAAAC AAACAGGCTG
    CTGGAGTCTC AGCTTCAGTC ACAGAAGAAG AGCCACGAGA ACGAGCTGGA ATCGCTGCGA
    GGTGAGATCC AAGGTCTGAA GGAAGAAAAC AATCGCCAGC AGCAGCTCCT GGCACAGAAC
    CTGCAGCTGC CTCCAGAGGC CCGCATCGAA GCCAGTCTCC AGCATGAGAT CACCCGGCTG
    ACGAATGAGA ACTTGGATTT AATGGAGCAG CTTGAAAAGC AAGACAAAAC TGTTCGCAAG
    TTAAAGAAAC AGTTGAAAGT ATTTGCTAAG AAGATCGGTG AACTTGAAGT GGGCCAGATG
    GAGAACATAT CACCTGGACA AATCATTGAT GAACCTATTC GTCCAGTTAA CATTCCACGG
    AAAGAAAAAG ACTTCCAAGG GATGTTAGAA TATAAGAAGG AGGATGAACA AAAACTTGTG
    AAGAATCTTA TTTTAGAGCT GAAACCACGT GGAGTAGCTG TCAACCTGAT TCCAGGACTA
    CCAGCATATA TTTTGTTCAT GTGTGTACGT CATGCGGATT ACCTTAATGA TGACCAAAAA
    GTGCGGTCGT TGTTGACTTC CACTATCAAC GGCATCAAAA AAGTGTTGAA GAAAAGAGGT
    GATGACTTTG AAACAGTGTC CTTCTGGCTC TCTAACACCT GCCGATTTTT GCACTGTTTG
    AAGCAATACA GTGGAGAAGA GGGTTTTATG AAGCATAATA CACCTCGTCA AAATGAACAC
    TGCCTCACTA ATTTTGATTT AGCTGAATAC AGACAAGTGC TGAGCGACTT GGCTATTCAG
    ATTTACCAAC AACTTGTCCG AGTGCTGGAA AACATTCTGC AACCCATGAT TGTTTCAGGA
    ATGCTGGAGC ATGAGACAAT CCAAGGTGTC TCGGGGGTGA AACCAACAGG GCTGCGGAAG
    AGAACATCCA GCATTGCTGA TGAAGGAACC TACACTTTGG ACTCCATTAT TCGGCAGCTG
    AACTCTTTCC ATTCAGTGAT GTGTCAGCAC GGAATGGATC CAGAGCTGAT CAAACAGGTT
    GTCAAGCAGA TGTTCTACAT CATTGGGGCT GTAACGCTTA ACAATCTTCT CCTGCGCAAG
    GACATGTGCT CGTGGAGCAA AGGAATGCAG ATAAGATACA ATGTGAGTCA ACTGGAAGAA
    TGGCTACGTG ATAAAAATCT GATGAATAGT GGGGCTAAAG AAACACTGGA ACCCCTCATC
    CAGGCTGCAC AGTTGTTGCA AGTGAAAAAG AAAACAGATG AAGATGCAGA AGCCATTTGT
    TCGATGTGCA ATGCACTGAC TACAGCCCAG ATTGTAAAAG TTCTGAATTT GTATACTCCA
    GTTAATGAGT TTGAAGAGAG AGTCTTGGTA TCGTTTATAC GTACAATACA GATGCGTCTG
    AGAGACAGGA AGGACTCTCC TCAGTTGCTC ATGGATGCTA AACATATCTT TCCTGTTACT
    TTTCCGTTTA ATCCATCCTC TCTGGCATTA GAAACCATTC AGATCCCAGC CAGCTTGGGC
    CTGGGCTTCA TATCACGTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66949
    Clone ID Related Accession (Same CDS sequence) XM_014944633.1
    Accession Version XM_014944633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5478bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGA ATACCATGAC AGATGCTACA ATCCTCTTGG AGGATGTACA AAAGATGAAA
    GACAAAGGAG AAATAGCACA GGCGTATATT GGACTGAAGG AAACAAACAG GCTGCTGGAG
    TCTCAGCTTC AGTCACAGAA GAAGAGCCAC GAGAACGAGC TGGAATCGCT GCGAGGTGAG
    ATCCAAGGTC TGAAGGAAGA AAACAATCGC CAGCAGCAGC TCCTGGCACA GAACCTGCAG
    CTGCCTCCAG AGGCCCGCAT CGAAGCCAGT CTCCAGCATG AGATCACCCG GCTGACGAAT
    GAGAACTTGG ATTTAATGGA GCAGCTTGAA AAGCAAGACA AAACTGTTCG CAAGTTAAAG
    AAACAGTTGA AAGTATTTGC TAAGAAGATC GGTGAACTTG AAGTGGGCCA GATGGAGAAC
    ATATCACCTG GACAAATCAT TGATGAACCT ATTCGTCCAG TTAACATTCC ACGGAAAGAA
    AAAGACTTCC AAGGGATGTT AGAATATAAG AAGGAGGATG AACAAAAACT TGTGAAGAAT
    CTTATTTTAG AGCTGAAACC ACGTGGAGTA GCTGTCAACC TGATTCCAGG ACTACCAGCA
    TATATTTTGT TCATGTGTGT ACGTCATGCG GATTACCTTA ATGATGACCA AAAAGTGCGG
    TCGTTGTTGA CTTCCACTAT CAACGGCATC AAAAAAGTGT TGAAGAAAAG AGGTGATGAC
    TTTGAAACAG TGTCCTTCTG GCTCTCTAAC ACCTGCCGAT TTTTGCACTG TTTGAAGCAA
    TACAGTGGAG AAGAGGGTTT TATGAAGCAT AATACACCTC GTCAAAATGA ACACTGCCTC
    ACTAATTTTG ATTTAGCTGA ATACAGACAA GTGCTGAGCG ACTTGGCTAT TCAGATTTAC
    CAACAACTTG TCCGAGTGCT GGAAAACATT CTGCAACCCA TGATTGTTTC AGGAATGCTG
    GAGCATGAGA CAATCCAAGG TGTCTCGGGG GTGAAACCAA CAGGGCTGCG GAAGAGAACA
    TCCAGCATTG CTGATGAAGG AACCTACACT TTGGACTCCA TTATTCGGCA GCTGAACTCT
    TTCCATTCAG TGATGTGTCA GCACGGAATG GATCCAGAGC TGATCAAACA GGTTGTCAAG
    CAGATGTTCT ACATCATTGG GGCTGTAACG CTTAACAATC TTCTCCTGCG CAAGGACATG
    TGCTCGTGGA GCAAAGGAAT GCAGATAAGA TACAATGTGA GTCAACTGGA AGAATGGCTA
    CGTGATAAAA ATCTGATGAA TAGTGGGGCT AAAGAAACAC TGGAACCCCT CATCCAGGCT
    GCACAGTTGT TGCAAGTGAA AAAGAAAACA GATGAAGATG CAGAAGCCAT TTGTTCGATG
    TGCAATGCAC TGACTACAGC CCAGATTGTA AAAGTTCTGA ATTTGTATAC TCCAGTTAAT
    GAGTTTGAAG AGAGAGTCTT GGTATCGTTT ATACGTACAA TACAGATGCG TCTGAGAGAC
    AGGAAGGACT CTCCTCAGTT GCTCATGGAT GCTAAACATA TCTTTCCTGT TACTTTTCCG
    TTTAATCCAT CCTCTCTGGC ATTAGAAACC ATTCAGATCC CAGCCAGCTT GGGCCTGGGC
    TTCATATCAC GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800119.1
    CDS151..5628
    Translation

    Target ORF information:

    RefSeq Version XM_014944633.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGA ATACCATGAC AGATGCTACA ATCCTCTTGG AGGATGTACA AAAGATGAAA
    GACAAAGGAG AAATAGCACA GGCGTATATT GGACTGAAGG AAACAAACAG GCTGCTGGAG
    TCTCAGCTTC AGTCACAGAA GAAGAGCCAC GAGAACGAGC TGGAATCGCT GCGAGGTGAG
    ATCCAAGGTC TGAAGGAAGA AAACAATCGC CAGCAGCAGC TCCTGGCACA GAACCTGCAG
    CTGCCTCCAG AGGCCCGCAT CGAAGCCAGT CTCCAGCATG AGATCACCCG GCTGACGAAT
    GAGAACTTGG ATTTAATGGA GCAGCTTGAA AAGCAAGACA AAACTGTTCG CAAGTTAAAG
    AAACAGTTGA AAGTATTTGC TAAGAAGATC GGTGAACTTG AAGTGGGCCA GATGGAGAAC
    ATATCACCTG GACAAATCAT TGATGAACCT ATTCGTCCAG TTAACATTCC ACGGAAAGAA
    AAAGACTTCC AAGGGATGTT AGAATATAAG AAGGAGGATG AACAAAAACT TGTGAAGAAT
    CTTATTTTAG AGCTGAAACC ACGTGGAGTA GCTGTCAACC TGATTCCAGG ACTACCAGCA
    TATATTTTGT TCATGTGTGT ACGTCATGCG GATTACCTTA ATGATGACCA AAAAGTGCGG
    TCGTTGTTGA CTTCCACTAT CAACGGCATC AAAAAAGTGT TGAAGAAAAG AGGTGATGAC
    TTTGAAACAG TGTCCTTCTG GCTCTCTAAC ACCTGCCGAT TTTTGCACTG TTTGAAGCAA
    TACAGTGGAG AAGAGGGTTT TATGAAGCAT AATACACCTC GTCAAAATGA ACACTGCCTC
    ACTAATTTTG ATTTAGCTGA ATACAGACAA GTGCTGAGCG ACTTGGCTAT TCAGATTTAC
    CAACAACTTG TCCGAGTGCT GGAAAACATT CTGCAACCCA TGATTGTTTC AGGAATGCTG
    GAGCATGAGA CAATCCAAGG TGTCTCGGGG GTGAAACCAA CAGGGCTGCG GAAGAGAACA
    TCCAGCATTG CTGATGAAGG AACCTACACT TTGGACTCCA TTATTCGGCA GCTGAACTCT
    TTCCATTCAG TGATGTGTCA GCACGGAATG GATCCAGAGC TGATCAAACA GGTTGTCAAG
    CAGATGTTCT ACATCATTGG GGCTGTAACG CTTAACAATC TTCTCCTGCG CAAGGACATG
    TGCTCGTGGA GCAAAGGAAT GCAGATAAGA TACAATGTGA GTCAACTGGA AGAATGGCTA
    CGTGATAAAA ATCTGATGAA TAGTGGGGCT AAAGAAACAC TGGAACCCCT CATCCAGGCT
    GCACAGTTGT TGCAAGTGAA AAAGAAAACA GATGAAGATG CAGAAGCCAT TTGTTCGATG
    TGCAATGCAC TGACTACAGC CCAGATTGTA AAAGTTCTGA ATTTGTATAC TCCAGTTAAT
    GAGTTTGAAG AGAGAGTCTT GGTATCGTTT ATACGTACAA TACAGATGCG TCTGAGAGAC
    AGGAAGGACT CTCCTCAGTT GCTCATGGAT GCTAAACATA TCTTTCCTGT TACTTTTCCG
    TTTAATCCAT CCTCTCTGGC ATTAGAAACC ATTCAGATCC CAGCCAGCTT GGGCCTGGGC
    TTCATATCAC GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa66950
    Clone ID Related Accession (Same CDS sequence) XM_014944634.1
    Accession Version XM_014944634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-08
    Organism Calidris pugnax(ruff)
    Product unconventional myosin-Va isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGG AGGATAAGAA TACCATGACA GATGCTACAA TCCTCTTGGA GGATGTACAA
    AAGATGAAAG ACAAAGGAGA AATAGCACAG GCGTATATTG GACTGAAGGA AACAAACAGG
    CAATCTCCCC AGGACTATCA TATGCTGAAT GAGGACGGAG AACTTTGGCT GGTTTATGAA
    GGGTTAAAAC AAGCCAACAG GCTGCTGGAG TCTCAGCTTC AGTCACAGAA GAAGAGCCAC
    GAGAACGAGC TGGAATCGCT GCGAGGTGAG ATCCAAGGTC TGAAGGAAGA AAACAATCGC
    CAGCAGCAGC TCCTGGCACA GAACCTGCAG CTGCCTCCAG AGGCCCGCAT CGAAGCCAGT
    CTCCAGCATG AGATCACCCG GCTGACGAAT GAGAACTTGG ATTTAATGGA GCAGCTTGAA
    AAGCAAGACA AAACTGTTCG CAAGTTAAAG AAACAGTTGA AAGTATTTGC TAAGAAGATC
    GGTGAACTTG AAGTGGGCCA GATGGAGAAC ATATCACCTG GACAAATCAT TGATGAACCT
    ATTCGTCCAG TTAACATTCC ACGGAAAGAA AAAGACTTCC AAGGGATGTT AGAATATAAG
    AAGGAGGATG AACAAAAACT TGTGAAGAAT CTTATTTTAG AGCTGAAACC ACGTGGAGTA
    GCTGTCAACC TGATTCCAGG ACTACCAGCA TATATTTTGT TCATGTGTGT ACGTCATGCG
    GATTACCTTA ATGATGACCA AAAAGTGCGG TCGTTGTTGA CTTCCACTAT CAACGGCATC
    AAAAAAGTGT TGAAGAAAAG AGGTGATGAC TTTGAAACAG TGTCCTTCTG GCTCTCTAAC
    ACCTGCCGAT TTTTGCACTG TTTGAAGCAA TACAGTGGAG AAGAGGGTTT TATGAAGCAT
    AATACACCTC GTCAAAATGA ACACTGCCTC ACTAATTTTG ATTTAGCTGA ATACAGACAA
    GTGCTGAGCG ACTTGGCTAT TCAGATTTAC CAACAACTTG TCCGAGTGCT GGAAAACATT
    CTGCAACCCA TGATTGTTTC AGGAATGCTG GAGCATGAGA CAATCCAAGG TGTCTCGGGG
    GTGAAACCAA CAGGGCTGCG GAAGAGAACA TCCAGCATTG CTGATGAAGG AACCTACACT
    TTGGACTCCA TTATTCGGCA GCTGAACTCT TTCCATTCAG TGATGTGTCA GCACGGAATG
    GATCCAGAGC TGATCAAACA GGTTGTCAAG CAGATGTTCT ACATCATTGG GGCTGTAACG
    CTTAACAATC TTCTCCTGCG CAAGGACATG TGCTCGTGGA GCAAAGGAAT GCAGATAAGA
    TACAATGTGA GTCAACTGGA AGAATGGCTA CGTGATAAAA ATCTGATGAA TAGTGGGGCT
    AAAGAAACAC TGGAACCCCT CATCCAGGCT GCACAGTTGT TGCAAGTGAA AAAGAAAACA
    GATGAAGATG CAGAAGCCAT TTGTTCGATG TGCAATGCAC TGACTACAGC CCAGATTGTA
    AAAGTTCTGA ATTTGTATAC TCCAGTTAAT GAGTTTGAAG AGAGAGTCTT GGTATCGTTT
    ATACGTACAA TACAGATGCG TCTGAGAGAC AGGAAGGACT CTCCTCAGTT GCTCATGGAT
    GCTAAACATA TCTTTCCTGT TACTTTTCCG TTTAATCCAT CCTCTCTGGC ATTAGAAACC
    ATTCAGATCC CAGCCAGCTT GGGCCTGGGC TTCATATCAC GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014800120.1
    CDS151..5718
    Translation

    Target ORF information:

    RefSeq Version XM_014944634.1
    Organism Calidris pugnax(ruff)
    Definition Calidris pugnax myosin VA (MYO5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014944634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAGCCT CCGAGCTCTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA TCCAGAGGAG 
    GTCTGGAAGT CAGCAGAACT TCTCAAAGAT TATAAACCTG GAGATAAAGT CCTTCAACTT
    CGACTTGAAG AGGGGAAGGA CCTAGAATAT AGCCTAGATC CGAAGACCAA GGAACTCCCA
    CCCTTGCGAA ACCCTGACAT ACTTGTTGGT GAAAATGACC TCACAGCACT CAGTTATCTC
    CATGAACCAG CTGTTTTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTAATCTAT
    ACCTATTGTG GTATCGTCTT AGTGGCCATA AATCCTTATG AGCAACTGCC TATCTATGGC
    GAAGATATCA TCAATGCCTA CAGTGGCCAA AACATGGGGG ATATGGATCC ACATATCTTT
    GCGGTGGCTG AAGAAGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT
    GTCAGTGGGG AGTCTGGAGC CGGGAAGACC GTCTCTGCTA AGTATGCCAT GAGGTACTTT
    GCCACGGTCA GTGGGTCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCCTCCAAC
    CCCATCATGG AGTCTATTGG AAATGCCAAA ACTACAAGGA ATGACAACAG CAGTCGCTTT
    GGGAAATACA TTGAAATTGG TTTTGATAAG AGGTATCGAA TCATTGGTGC TAACATGAGA
    ACTTACCTCT TGGAAAAATC AAGAGTGGTG TTTCAGGCAG AAGAGGAGAG AAATTACCAC
    ATCTTTTACC AACTATGTGC CTCTGCAGCA TTACCCGAGT TTAAAACTCT ACGATTAGGG
    AATGCAAATT ACTTTCACTA TACGAAGCAA GGTGGAAGCC CTGTGATTGA TGGTGTCAAT
    GATGCTAAGG AAATGGTAAA CACCAGGCAG GCCTGCACTT TGCTAGGGAT TAGTGATACC
    TACCAGATGG GCATTTTTCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT
    GCGTCTCGGG ATTCCGACAG CTGCACTGTT CCTCCCAAGC ATGAACCCCT CACCATCTTC
    TGTGACCTCA TGGGTGTGGA GTATGAAGAG ATGGCCCACT GGCTTTGCCA TAGGAAGCTC
    GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA
    GATGCACTTG CCAAACATAT CTACGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC
    AAAGCCCTTC ACGCTACTGT AAAACAGCAT TCCTTCATTG GAGTGTTGGA CATTTATGGA
    TTCGAGACAT TCGAAATCAA CAGCTTTGAA CAGTTCTGTA TCAACTATGC CAACGAAAAA
    CTGCAGCAGC AGTTCAATAT GCATGTCTTT AAGCTGGAAC AAGAAGAGTA TATGAAGGAA
    CAAATACCAT GGACCTTGAT TGATTTCTAC GACAATCAGC CATGCATCAA CCTCATAGAG
    GCCAAAATGG GAGTTCTGGA TCTGTTGGAT GAGGAGTGCA AGATGCCAAA AGGCTCGGAT
    GACACTTGGG CCCAAAAACT ATACAATACT CATTTGAATA AATGTGCCCT CTTTGAGAAG
    CCACGTTTGT CAAATAAGGC TTTTATCATC AAGCACTTTG CTGACAAGGT TGAATATCAA
    TGCGAAGGCT TTTTGGAAAA GAATAAAGAC ACGGTTTATG AAGAACAAAT TAAGGTCCTA
    AAATCAAGTA AGTTTAAGCT GCTGCCAGAG TTATTCCAGG ATGAGGAGAA GGTCCTTAGT
    CCCACGTCAG CCACCCCTTC AGGGCGCGTA CCTTTGTCTC GAACAGCTGT AAAACCAGCC
    AAGGCCCGGC CAGGTCAAGC CAGCAAGGAG CATAAGAAAA CTGTGGGACA TCAGTTTCGA
    AACTCTCTTC ACCTGCTGAT GGAAACCCTG AATGCTACAA CCCCACACTA CGTGCGCTGT
    ATTAAGCCCA ACGACTTCAA GTTTCCGTTC ACATTCGATG AAAAGCGGGC AGTGCAGCAG
    CTGAGAGCTT GTGGTGTCCT GGAGACCATC CGAATCAGTG CAGCTGGCTT CCCTTCCAGG
    TGGACGTACC AAGAGTTCTT CAGCCGTTAC CGTGTTCTGA TGAAGCAGAG AGATGTCCTT
    AGTGACCGAA AGCAGACATG TAAAAATGTC CTGGAAAAGC TAATTCTGGA CAAGGATAAG
    TACCAGTTTG GTAAGACAAA AATATTTTTC CGGGCTGGTC AAGTAGCCTA TCTGGAAAAA
    ATAAGGGCAG ATAAGTTGAG AGCTGCCTGT ATCCGCATCC AAAAGACAAT CCGAGGCTGG
    CTGATGCGAA AGAAGTACGT GCGTATGAGG AAGGCTGCCA TCACCATTCA GAGACATGTC
    AGAGGGTACC AAGCACGATG CTATGCCAAG TTTCTGCGGA GAACACGGGC TGCCATCACT
    ATTCAGAAGT TCCAGCGCAT GTATGTGGTC CGCAAAAGAT ACCAATGCAT GCGAGATGCT
    ACTATTGCTC TTCAGGCTCT CTTAAGAGGT TACATGGCCA GGAACAAATA CCAAATGATG
    CTTCGGGAGC ACAAGTCTAT CATTATTCAG AAACATATAA GAGGCTGGCT GGCCCGAGTG
    CACTACCGTA GGACCTTGAA GGCAATTGTT TACTTGCAAT GCTGTTACCG GCGCATGATG
    GCCAAAAGGG AGCTGAAGAA ACTGAAGATA GAGGCCCGGT CTGTAGAACG CTACAAGAAG
    CTTCACATTG GCTTAGAGAA CAAGATCATG CAGCTGCAGC GAAAAATTGA TGAGCAGAAC
    AAAGAGTACA AATCTCTGCT GGAGAAGCTG AGCAACCTGG AGATCACGTA CAGTACAGAG
    ACAGAGAAGC TGCGGAGTGA CGTGGAGAGG CTTCGGATGA GTGAGGAGGA GGCTAAGAAC
    GCGACCAACC GTGTCCTCAG CCTGCAGGAG GAGATTGCCA AGCTCCGGAA GGAGCTGCAC
    CAAACTCAGA CTGAGAAGAA GACCATTGAG GAACGGGCAG ACAAATACAA ACATGAAACC
    GAACAGCTGG TTTCAGAGCT GAAAGAGCAG AACACGTTAC TGAAAACAGA GAAGGAGGAG
    CTGAACCGTC GCATCCATGA CCAGGCAAAG GAAATAACAG AGACGATGGA GAAGAAGCTA
    GTGGAGGAAA CCAAGCAGCT AGAGCTAGAT CTGAATGATG AACGGTTACG GTACCAGAAC
    CTGCTGAATG AGTTCAGCCG TTTAGAGGAG CGGTATGATG ATCTCAAGGA TGAAATGAAC
    TTAATGGTGA GCATCCCCAA GCCTGGGCAC AAAAGAACGG ATTCAACTCA CAGTAGCAAT
    GAATCTGAAT ACACTTTTAG CTCTGAGATC ACAGAAGCAG AAGACTTACC AGTGAGGATG
    GAGGAACCAA GTGAGAAAAA GGCACCATTG GATATGTCTC TGTTCCTCAA GCTGCAGAAA
    CGGGTTACGG AGCTGGAGCA AGAAAAGCAG TCCCTGCAGG ATGAACTGGA CAGAAAGGAA
    GAACAAGCTC TGCGTGCTAA AGCTAAGGAG GAAGAAAGGC CTCCAATGAG AGGTGCTGAG
    TTGGAGTACG AGTCACTCAA GCGCCAAGAA CTTGAATCTG AGAATAAAAA GCTGAAGAAT
    GAGTTGAATG AGCTGCAGAA GGCCCTCACA GAAACACGAT CTCCAGAGGT AACTGCTCCT
    GGTGCCCCTG CGTACAGAGT CCTCCTGGAT CAGCTGACGT CAGTCAGTGA AGAGCTCGAA
    GTACGCAAGG AAGAAGTCCT CATCCTGAGG TCTCAGCTGG TTAGCCAGAA AGAGGCTATT
    CAACCCAAGG AGGATAAGAA TACCATGACA GATGCTACAA TCCTCTTGGA GGATGTACAA
    AAGATGAAAG ACAAAGGAGA AATAGCACAG GCGTATATTG GACTGAAGGA AACAAACAGG
    CAATCTCCCC AGGACTATCA TATGCTGAAT GAGGACGGAG AACTTTGGCT GGTTTATGAA
    GGGTTAAAAC AAGCCAACAG GCTGCTGGAG TCTCAGCTTC AGTCACAGAA GAAGAGCCAC
    GAGAACGAGC TGGAATCGCT GCGAGGTGAG ATCCAAGGTC TGAAGGAAGA AAACAATCGC
    CAGCAGCAGC TCCTGGCACA GAACCTGCAG CTGCCTCCAG AGGCCCGCAT CGAAGCCAGT
    CTCCAGCATG AGATCACCCG GCTGACGAAT GAGAACTTGG ATTTAATGGA GCAGCTTGAA
    AAGCAAGACA AAACTGTTCG CAAGTTAAAG AAACAGTTGA AAGTATTTGC TAAGAAGATC
    GGTGAACTTG AAGTGGGCCA GATGGAGAAC ATATCACCTG GACAAATCAT TGATGAACCT
    ATTCGTCCAG TTAACATTCC ACGGAAAGAA AAAGACTTCC AAGGGATGTT AGAATATAAG
    AAGGAGGATG AACAAAAACT TGTGAAGAAT CTTATTTTAG AGCTGAAACC ACGTGGAGTA
    GCTGTCAACC TGATTCCAGG ACTACCAGCA TATATTTTGT TCATGTGTGT ACGTCATGCG
    GATTACCTTA ATGATGACCA AAAAGTGCGG TCGTTGTTGA CTTCCACTAT CAACGGCATC
    AAAAAAGTGT TGAAGAAAAG AGGTGATGAC TTTGAAACAG TGTCCTTCTG GCTCTCTAAC
    ACCTGCCGAT TTTTGCACTG TTTGAAGCAA TACAGTGGAG AAGAGGGTTT TATGAAGCAT
    AATACACCTC GTCAAAATGA ACACTGCCTC ACTAATTTTG ATTTAGCTGA ATACAGACAA
    GTGCTGAGCG ACTTGGCTAT TCAGATTTAC CAACAACTTG TCCGAGTGCT GGAAAACATT
    CTGCAACCCA TGATTGTTTC AGGAATGCTG GAGCATGAGA CAATCCAAGG TGTCTCGGGG
    GTGAAACCAA CAGGGCTGCG GAAGAGAACA TCCAGCATTG CTGATGAAGG AACCTACACT
    TTGGACTCCA TTATTCGGCA GCTGAACTCT TTCCATTCAG TGATGTGTCA GCACGGAATG
    GATCCAGAGC TGATCAAACA GGTTGTCAAG CAGATGTTCT ACATCATTGG GGCTGTAACG
    CTTAACAATC TTCTCCTGCG CAAGGACATG TGCTCGTGGA GCAAAGGAAT GCAGATAAGA
    TACAATGTGA GTCAACTGGA AGAATGGCTA CGTGATAAAA ATCTGATGAA TAGTGGGGCT
    AAAGAAACAC TGGAACCCCT CATCCAGGCT GCACAGTTGT TGCAAGTGAA AAAGAAAACA
    GATGAAGATG CAGAAGCCAT TTGTTCGATG TGCAATGCAC TGACTACAGC CCAGATTGTA
    AAAGTTCTGA ATTTGTATAC TCCAGTTAAT GAGTTTGAAG AGAGAGTCTT GGTATCGTTT
    ATACGTACAA TACAGATGCG TCTGAGAGAC AGGAAGGACT CTCCTCAGTT GCTCATGGAT
    GCTAAACATA TCTTTCCTGT TACTTTTCCG TTTAATCCAT CCTCTCTGGC ATTAGAAACC
    ATTCAGATCC CAGCCAGCTT GGGCCTGGGC TTCATATCAC GTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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