SCN1A cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN1A
Entrez Gene ID	106895134
Full Name	sodium voltage-gated channel alpha subunit 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014954268.1	XP_014809754.1	sodium channel protein type 1 subunit alpha isoform X1
XM_014954269.1	XP_014809755.1	sodium channel protein type 1 subunit alpha isoform X2
XM_014954271.1	XP_014809757.1	sodium channel protein type 1 subunit alpha isoform X2
XM_014954275.1	XP_014809761.1	sodium channel protein type 1 subunit alpha isoform X5
XM_014954270.1	XP_014809756.1	sodium channel protein type 1 subunit alpha isoform X3
XM_014954272.1	XP_014809758.1	sodium channel protein type 1 subunit alpha isoform X2
XM_014954276.1	XP_014809762.1	sodium channel protein type 1 subunit alpha isoform X6
XM_014954273.1	XP_014809759.1	sodium channel protein type 1 subunit alpha isoform X4

Pathways from BioSystems
Gene Ontology

The following SCN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa74028	XM_014954268.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74028	XM_014954271.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74028	XM_014954269.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74028	XM_014954272.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74029	XM_014954270.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74029	XM_014954276.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74030	XM_014954273.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa74031	XM_014954275.1 Latest version!	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN1A ( XM_014954268.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809754.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa74028
Clone ID Related Accession (Same CDS sequence)	XM_014954268.1 , XM_014954269.1 , XM_014954271.1 , XM_014954272.1
Accession Version	XM_014954268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809754.1
CDS	55..6099
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954268.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954268.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954269.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809755.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

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CloneID	OCa74028
Clone ID Related Accession (Same CDS sequence)	XM_014954268.1 , XM_014954269.1 , XM_014954271.1 , XM_014954272.1
Accession Version	XM_014954269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809755.1
CDS	171..6215
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954269.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954269.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954271.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809757.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

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CloneID	OCa74028
Clone ID Related Accession (Same CDS sequence)	XM_014954268.1 , XM_014954269.1 , XM_014954271.1 , XM_014954272.1
Accession Version	XM_014954271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809757.1
CDS	251..6295
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954271.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954271.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954272.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809758.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.

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CloneID	OCa74028
Clone ID Related Accession (Same CDS sequence)	XM_014954268.1 , XM_014954269.1 , XM_014954271.1 , XM_014954272.1
Accession Version	XM_014954272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809758.1
CDS	181..6225
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954272.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954272.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954270.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809756.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

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CloneID	OCa74029
Clone ID Related Accession (Same CDS sequence)	XM_014954270.1 , XM_014954276.1
Accession Version	XM_014954270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809756.1
CDS	55..6099
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954270.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954270.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954276.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809762.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X8, mRNA.

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CloneID	OCa74029
Clone ID Related Accession (Same CDS sequence)	XM_014954270.1 , XM_014954276.1
Accession Version	XM_014954276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809762.1
CDS	171..6215
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954276.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954276.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGTG 
ATAATAGATA AACCAGCTAC TGATGACAAT GGCACCACTA CAGAAGCAGA AATGAGGAAG 
AGAATATCAG GCTCTTTCCA CGTTTCTATG GACTTCCTCG AAGATCCAGC TTTAAGGGAA 
AGAGCCATGA GTATTGCTAG CATCCTCACA AACACTGTGG AAGAACTTGA AGAATCAAGA 
CAGAAATGCC CACCATGTTG GTATAAATTT GCAAATATTT TCTTGATCTG GGACTGCAGT 
CCACATTGGT TAAAAGTAAA ACATATTGTC AACTTAGTGG TAATGGACCC ATTTGTTGAC 
CTGGCTATTA CAATTTGCAT TGTTTTAAAT ACACTCTTTA TGGCTATGGA ACATTATCCA 
ATGACCGATG AATTCAATAA CGTACTTTCA GTTGGAAATC TGGTCTTCAC TGGAATCTTC 
ACAGCAGAAA TGTTTCTCAA GATAATTGCA ATGGATCCTT ACTATTATTT CCAGGAGGGT 
TGGAATATTT TTGATGGTTT TATTGTAACC CTTAGCTTGG TTGAGCTTGG TCTTGCAGAT 
GTGGAAGGAC TCTCAGTTCT TCGATCATTC AGATTGTTAC GAGTTTTCAA ATTGGCAAAA 
TCTTGGCCAA CCCTAAATAT GCTGATAAAA ATTATTGGTA ATTCTGTGGG AGCTCTGGGG 
AACCTGACCT TGGTGTTGGC CATCATTGTG TTCATTTTCG CTGTGGTTGG GATGCAGCTG 
TTTGGGAAAA ACTATAAGGA ATGTGTCTGC AAAATTGCAA GTGACTGTGT TCTTCCACGC 
TGGCATATGC AAGATTTTTT TCACTCTTTT CTGATCGTAT TTCGAGTGCT GTGTGGAGAA 
TGGATAGAAA CGATGTGGGA CTGCATGGAG GTTGCTGGCC AAGCCATGTG CCTTACGGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTACTGA ACCTATTTTT GGCCTTGCTC 
TTGAGCTCAT TTAGTGCAGA CAACCTTGCC GTGACAGATG ATGACAATGA AATGAATAAT 
CTCCAAATTG CTGTAGCAAG AATGCAGAAG GGCATAGATT ATGTGAAGAG AAAAGCACGT 
GAATTCATCC AAAAAGCTTT TGTTAAAAAA CAAAAAGCTT TAGATGAAAT CAAACCACTT 
GAAGATTTGA ACAACAAAAA TAGCTGTATT TCTAACCATA CAACCGTGGA TCTAAGCAAG 
GACCATGACT ATATTAAAGA TGTGAATGGA ACAACTAGTG GCATAGGCAC AGGTAGCAGT 
GTGGAAAAAT ACATAATTGA CGAAAGTGAT TACATGTCAT TCATAAACAA CCCAAGTCTC 
ACTGTGACAG TGCCCATTGC TGTGGGTGAA TCCGACTTTG AAAATCTAAA CACAGAGGAA 
TTTAGTAGTG AATCAGACCT GGAAGAAAGC AAAGAGAAGT TGAATTCGTC TAGTTCATCC 
GAAGGTAGCA CAGTTGATAT AGGACTTCCT GCAGAAGAGC AACCAGAAGC TGAACCTGAA 
GAAGCCCAGG AGCCAGAGGC CTGCTTCACA GAAGGCTGTG TACGAAGGTT CAAGTGCTGT 
CAAGTAAGTA TAGAAGAAGG AAGAGGAAAG CAGTGGTGGA ACCTTAGAAA AACCTGCTTC 
AGGATTGTTG AACACAACTG GTTCGAGACT TTCATTGTCT TTATGATTCT CCTCAGTAGT 
GGAGCTCTGG CTTTTGAAGA CATATATATT GAACAGCGTA AAACTATCAA GACTATGCTG 
GAATATGCTG ACAAGGTCTT CACTTATATC TTCATTCTGG AAATGCTGCT GAAGTGGGTG 
GCCTATGGTT ATCAGACATA CTTTACTAAT GCCTGGTGCT GGCTGGACTT CCTGATTGTC 
GATGTCTCTT TGGTTAGCTT AACAGCAAAT GCATTGGGTT ACTCTGAACT TGGTGCCATT 
AAGTCCCTTA GGACACTAAG AGCTTTGAGA CCTCTAAGAG CTTTGTCACG ATTTGAAGGC 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA CGTGCTTCTC 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGAG TAAATCTGTT TGCTGGGAAG 
TTCTACTACT GTGTTAACAC CACAACTGAT GAAAGGTTTG AAGTCGACCA AATCAACAAT 
TTTAGTCAGT GCGAGGAGCT CATAAAAAAC AATCAAAGTG CCCGGTGGAA AAACGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTGTCTTTAC TTCAAGTAGC TACATTTAAA 
GGCTGGATGG ATATTATGTA TGCAGCAATT GACTCAAGAG ATGTATTACA GCAACCCAAG 
TATGAAGACA ACCTGTACAT GTATTTGTAC TTTGTCATCT TTATCATTTT TGGGTCTTTT 
TTCACCCTGA ACCTGTTCAT TGGTGTCATT ATTGATAACT TCAATCAGCA AAAAAAGAAG 
TTTGGAGGTC AAGACATATT TATGACAGAA GAGCAGAAGA AATACTACAA TGCAATGAAA 
AAGCTGGGAT CCAAAAAACC ACAAAAACCT ATACCAAGAC CAGGGAACAA ATTCCAAGGC 
ATGGTCTTTG ATTTTGTGAC ACAGCAAGTC TTTGACATCA GCATCATGAT TCTTATTTGT 
CTTAACATGG TGACCATGAT GGTAGAAACT GATGACCAGA GTAAAGAAAT GGAAAATATT 
TTATACTGGA TAAACCTGGT GTTCATTGTC CTTTTTACTG GAGAATGTGT CCTCAAATTA 
ATTTCACTTC GCCATTACTA TTTCACTATA GGCTGGAACA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCAA TAGTAGGTAT GTTCTTAGCC GAGATGATTG AGAAATATTT TGTCTCCCCC 
ACGCTATTCC GAGTGATCCG ACTCGCCAGA ATCGGTCGAA TCCTGCGTCT CATTAAAGGC 
GCCAAGGGAA TCCGCACTCT GCTTTTTGCT TTGATGATGT CTCTTCCTGC TTTGTTCAAC 
ATTGGTCTGC TGCTCTTCCT GGTCATGTTT ATCTATGCCA TCTTTGGCAT GTCCAACTTT 
GCCTACGTTA AGAGGGAAGC TGGGATTGAT GACATGTTCA ACTTTGAAAC GTTTGGCAAC 
AGCATGATCT GCCTCTTTCA AATCACCACA TCTGCTGGCT GGGATGGTTT GCTGGCCCCA 
ATTCTTAACA GTGGGGAGCC AGACTGTGAC CCTAACAAAG CTCATCCGGG GAGCTCTGTG 
AAAGGCGACT GTGGCAACCC TTCTGTTGGG ATTTTCTTCT TTGTCAGCTA CATTATCATA 
TCATTTCTGG TAGTGGTGAA CATGTACATT GCTGTGATTT TGGAGAATTT TGGTGTTGCT 
ACTGAAGAAA GTGCTGAACC CTTGAGCGAG GATGACTTTG AGATGTTCTA CGAAGTCTGG 
GAGAAGTTTG ATCCCGATGC AACACAATTC ATGGAATTTG AGAAATTGTC TGATTTTGCA 
GCAGCACTTG AGCCTCCTCT TCATCTACCC AAACCAAACA AAGTCCAGCT TATTGCAATG 
GATCTGCCCA TGGTAAGCGG CGACAGAATT CACTGCCTTG ACATCTTGTT TGCTTTCACA 
AAGCGTGTTT TGGGGGAAAG TGGGGAGATG GACGCCCTCA GAATACAGAT GGAAGATCGG 
TTTATGGCAT CCAATCCTTC AAAAGCTTCC TATGAACCAA TTACAACCAC ATTAAAAAGA 
AAACAGGAGG AAGTATCTGC TGTCATTATT CAACGGGCTT ATAGGCGTCA CCTTTTAAAA 
CGAACAGTAA AACAAGCTTC CTTTATCTAT AACAAAAACA AAACGGAAGG AGGGGCTGGT 
CAGGGTATGA AAGATGAAAT CCTTATTGAC AAGTTAAATG AAAACTCCTT TACAGAGAAA 
ACTGATATAA CTGCATCAAC AGCAGTTTGT CCACCTTCTT ATGATCACGT AACAAGGCCA 
GACAAAAAAA AGCATGAAAA GGAAGATAAA GGAGGTCAGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954273.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809759.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X6, mRNA.

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CloneID	OCa74030
Clone ID Related Accession (Same CDS sequence)	XM_014954273.1
Accession Version	XM_014954273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGGC 
ACCACTACAG AAGCAGAAAT GAGGAAGAGA ATATCAGGCT CTTTCCACGT TTCTATGGAC 
TTCCTCGAAG ATCCAGCTTT AAGGGAAAGA GCCATGAGTA TTGCTAGCAT CCTCACAAAC 
ACTGTGGAAG AACTTGAAGA ATCAAGACAG AAATGCCCAC CATGTTGGTA TAAATTTGCA 
AATATTTTCT TGATCTGGGA CTGCAGTCCA CATTGGTTAA AAGTAAAACA TATTGTCAAC 
TTAGTGGTAA TGGACCCATT TGTTGACCTG GCTATTACAA TTTGCATTGT TTTAAATACA 
CTCTTTATGG CTATGGAACA TTATCCAATG ACCGATGAAT TCAATAACGT ACTTTCAGTT 
GGAAATCTGG TCTTCACTGG AATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCAATG 
GATCCTTACT ATTATTTCCA GGAGGGTTGG AATATTTTTG ATGGTTTTAT TGTAACCCTT 
AGCTTGGTTG AGCTTGGTCT TGCAGATGTG GAAGGACTCT CAGTTCTTCG ATCATTCAGA 
TTGTTACGAG TTTTCAAATT GGCAAAATCT TGGCCAACCC TAAATATGCT GATAAAAATT 
ATTGGTAATT CTGTGGGAGC TCTGGGGAAC CTGACCTTGG TGTTGGCCAT CATTGTGTTC 
ATTTTCGCTG TGGTTGGGAT GCAGCTGTTT GGGAAAAACT ATAAGGAATG TGTCTGCAAA 
ATTGCAAGTG ACTGTGTTCT TCCACGCTGG CATATGCAAG ATTTTTTTCA CTCTTTTCTG 
ATCGTATTTC GAGTGCTGTG TGGAGAATGG ATAGAAACGA TGTGGGACTG CATGGAGGTT 
GCTGGCCAAG CCATGTGCCT TACGGTCTTC ATGATGGTCA TGGTGATTGG AAACCTAGTG 
GTACTGAACC TATTTTTGGC CTTGCTCTTG AGCTCATTTA GTGCAGACAA CCTTGCCGTG 
ACAGATGATG ACAATGAAAT GAATAATCTC CAAATTGCTG TAGCAAGAAT GCAGAAGGGC 
ATAGATTATG TGAAGAGAAA AGCACGTGAA TTCATCCAAA AAGCTTTTGT TAAAAAACAA 
AAAGCTTTAG ATGAAATCAA ACCACTTGAA GATTTGAACA ACAAAAATAG CTGTATTTCT 
AACCATACAA CCGTGGATCT AAGCAAGGAC CATGACTATA TTAAAGATGT GAATGGAACA 
ACTAGTGGCA TAGGCACAGG TAGCAGTGTG GAAAAATACA TAATTGACGA AAGTGATTAC 
ATGTCATTCA TAAACAACCC AAGTCTCACT GTGACAGTGC CCATTGCTGT GGGTGAATCC 
GACTTTGAAA ATCTAAACAC AGAGGAATTT AGTAGTGAAT CAGACCTGGA AGAAAGCAAA 
GAGAAGTTGA ATTCGTCTAG TTCATCCGAA GGTAGCACAG TTGATATAGG ACTTCCTGCA 
GAAGAGCAAC CAGAAGCTGA ACCTGAAGAA GCCCAGGAGC CAGAGGCCTG CTTCACAGAA 
GGCTGTGTAC GAAGGTTCAA GTGCTGTCAA GTAAGTATAG AAGAAGGAAG AGGAAAGCAG 
TGGTGGAACC TTAGAAAAAC CTGCTTCAGG ATTGTTGAAC ACAACTGGTT CGAGACTTTC 
ATTGTCTTTA TGATTCTCCT CAGTAGTGGA GCTCTGGCTT TTGAAGACAT ATATATTGAA 
CAGCGTAAAA CTATCAAGAC TATGCTGGAA TATGCTGACA AGGTCTTCAC TTATATCTTC 
ATTCTGGAAA TGCTGCTGAA GTGGGTGGCC TATGGTTATC AGACATACTT TACTAATGCC 
TGGTGCTGGC TGGACTTCCT GATTGTCGAT GTCTCTTTGG TTAGCTTAAC AGCAAATGCA 
TTGGGTTACT CTGAACTTGG TGCCATTAAG TCCCTTAGGA CACTAAGAGC TTTGAGACCT 
CTAAGAGCTT TGTCACGATT TGAAGGCATG AGGGTGGTTG TGAATGCCCT TTTAGGAGCA 
ATTCCATCCA TCATGAACGT GCTTCTCGTT TGTCTTATAT TCTGGCTAAT TTTCAGCATC 
ATGGGAGTAA ATCTGTTTGC TGGGAAGTTC TACTACTGTG TTAACACCAC AACTGATGAA 
AGGTTTGAAG TCGACCAAAT CAACAATTTT AGTCAGTGCG AGGAGCTCAT AAAAAACAAT 
CAAAGTGCCC GGTGGAAAAA CGTGAAAGTA AACTTTGATA ATGTAGGATT TGGGTATCTG 
TCTTTACTTC AAGTAGCTAC ATTTAAAGGC TGGATGGATA TTATGTATGC AGCAATTGAC 
TCAAGAGATG TATTACAGCA ACCCAAGTAT GAAGACAACC TGTACATGTA TTTGTACTTT 
GTCATCTTTA TCATTTTTGG GTCTTTTTTC ACCCTGAACC TGTTCATTGG TGTCATTATT 
GATAACTTCA ATCAGCAAAA AAAGAAGTTT GGAGGTCAAG ACATATTTAT GACAGAAGAG 
CAGAAGAAAT ACTACAATGC AATGAAAAAG CTGGGATCCA AAAAACCACA AAAACCTATA 
CCAAGACCAG GGAACAAATT CCAAGGCATG GTCTTTGATT TTGTGACACA GCAAGTCTTT 
GACATCAGCA TCATGATTCT TATTTGTCTT AACATGGTGA CCATGATGGT AGAAACTGAT 
GACCAGAGTA AAGAAATGGA AAATATTTTA TACTGGATAA ACCTGGTGTT CATTGTCCTT 
TTTACTGGAG AATGTGTCCT CAAATTAATT TCACTTCGCC ATTACTATTT CACTATAGGC 
TGGAACATTT TTGATTTTGT GGTTGTCATT CTCTCAATAG TAGGTATGTT CTTAGCCGAG 
ATGATTGAGA AATATTTTGT CTCCCCCACG CTATTCCGAG TGATCCGACT CGCCAGAATC 
GGTCGAATCC TGCGTCTCAT TAAAGGCGCC AAGGGAATCC GCACTCTGCT TTTTGCTTTG 
ATGATGTCTC TTCCTGCTTT GTTCAACATT GGTCTGCTGC TCTTCCTGGT CATGTTTATC 
TATGCCATCT TTGGCATGTC CAACTTTGCC TACGTTAAGA GGGAAGCTGG GATTGATGAC 
ATGTTCAACT TTGAAACGTT TGGCAACAGC ATGATCTGCC TCTTTCAAAT CACCACATCT 
GCTGGCTGGG ATGGTTTGCT GGCCCCAATT CTTAACAGTG GGGAGCCAGA CTGTGACCCT 
AACAAAGCTC ATCCGGGGAG CTCTGTGAAA GGCGACTGTG GCAACCCTTC TGTTGGGATT 
TTCTTCTTTG TCAGCTACAT TATCATATCA TTTCTGGTAG TGGTGAACAT GTACATTGCT 
GTGATTTTGG AGAATTTTGG TGTTGCTACT GAAGAAAGTG CTGAACCCTT GAGCGAGGAT 
GACTTTGAGA TGTTCTACGA AGTCTGGGAG AAGTTTGATC CCGATGCAAC ACAATTCATG 
GAATTTGAGA AATTGTCTGA TTTTGCAGCA GCACTTGAGC CTCCTCTTCA TCTACCCAAA 
CCAAACAAAG TCCAGCTTAT TGCAATGGAT CTGCCCATGG TAAGCGGCGA CAGAATTCAC 
TGCCTTGACA TCTTGTTTGC TTTCACAAAG CGTGTTTTGG GGGAAAGTGG GGAGATGGAC 
GCCCTCAGAA TACAGATGGA AGATCGGTTT ATGGCATCCA ATCCTTCAAA AGCTTCCTAT 
GAACCAATTA CAACCACATT AAAAAGAAAA CAGGAGGAAG TATCTGCTGT CATTATTCAA 
CGGGCTTATA GGCGTCACCT TTTAAAACGA ACAGTAAAAC AAGCTTCCTT TATCTATAAC 
AAAAACAAAA CGGAAGGAGG GGCTGGTCAG GGTATGAAAG ATGAAATCCT TATTGACAAG 
TTAAATGAAA ACTCCTTTAC AGAGAAAACT GATATAACTG CATCAACAGC AGTTTGTCCA 
CCTTCTTATG ATCACGTAAC AAGGCCAGAC AAAAAAAAGC ATGAAAAGGA AGATAAAGGA 
GGTCAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809759.1
CDS	55..6066
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954273.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954273.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCATATG TGACAGAGTT TGTGGACCTG GGCAATGTCT CAGCGTTGAG AACATTCAGA 
GTTCTCCGAG CTTTGAAAAC AATATCAGTC ATTCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGGC 
ACCACTACAG AAGCAGAAAT GAGGAAGAGA ATATCAGGCT CTTTCCACGT TTCTATGGAC 
TTCCTCGAAG ATCCAGCTTT AAGGGAAAGA GCCATGAGTA TTGCTAGCAT CCTCACAAAC 
ACTGTGGAAG AACTTGAAGA ATCAAGACAG AAATGCCCAC CATGTTGGTA TAAATTTGCA 
AATATTTTCT TGATCTGGGA CTGCAGTCCA CATTGGTTAA AAGTAAAACA TATTGTCAAC 
TTAGTGGTAA TGGACCCATT TGTTGACCTG GCTATTACAA TTTGCATTGT TTTAAATACA 
CTCTTTATGG CTATGGAACA TTATCCAATG ACCGATGAAT TCAATAACGT ACTTTCAGTT 
GGAAATCTGG TCTTCACTGG AATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCAATG 
GATCCTTACT ATTATTTCCA GGAGGGTTGG AATATTTTTG ATGGTTTTAT TGTAACCCTT 
AGCTTGGTTG AGCTTGGTCT TGCAGATGTG GAAGGACTCT CAGTTCTTCG ATCATTCAGA 
TTGTTACGAG TTTTCAAATT GGCAAAATCT TGGCCAACCC TAAATATGCT GATAAAAATT 
ATTGGTAATT CTGTGGGAGC TCTGGGGAAC CTGACCTTGG TGTTGGCCAT CATTGTGTTC 
ATTTTCGCTG TGGTTGGGAT GCAGCTGTTT GGGAAAAACT ATAAGGAATG TGTCTGCAAA 
ATTGCAAGTG ACTGTGTTCT TCCACGCTGG CATATGCAAG ATTTTTTTCA CTCTTTTCTG 
ATCGTATTTC GAGTGCTGTG TGGAGAATGG ATAGAAACGA TGTGGGACTG CATGGAGGTT 
GCTGGCCAAG CCATGTGCCT TACGGTCTTC ATGATGGTCA TGGTGATTGG AAACCTAGTG 
GTACTGAACC TATTTTTGGC CTTGCTCTTG AGCTCATTTA GTGCAGACAA CCTTGCCGTG 
ACAGATGATG ACAATGAAAT GAATAATCTC CAAATTGCTG TAGCAAGAAT GCAGAAGGGC 
ATAGATTATG TGAAGAGAAA AGCACGTGAA TTCATCCAAA AAGCTTTTGT TAAAAAACAA 
AAAGCTTTAG ATGAAATCAA ACCACTTGAA GATTTGAACA ACAAAAATAG CTGTATTTCT 
AACCATACAA CCGTGGATCT AAGCAAGGAC CATGACTATA TTAAAGATGT GAATGGAACA 
ACTAGTGGCA TAGGCACAGG TAGCAGTGTG GAAAAATACA TAATTGACGA AAGTGATTAC 
ATGTCATTCA TAAACAACCC AAGTCTCACT GTGACAGTGC CCATTGCTGT GGGTGAATCC 
GACTTTGAAA ATCTAAACAC AGAGGAATTT AGTAGTGAAT CAGACCTGGA AGAAAGCAAA 
GAGAAGTTGA ATTCGTCTAG TTCATCCGAA GGTAGCACAG TTGATATAGG ACTTCCTGCA 
GAAGAGCAAC CAGAAGCTGA ACCTGAAGAA GCCCAGGAGC CAGAGGCCTG CTTCACAGAA 
GGCTGTGTAC GAAGGTTCAA GTGCTGTCAA GTAAGTATAG AAGAAGGAAG AGGAAAGCAG 
TGGTGGAACC TTAGAAAAAC CTGCTTCAGG ATTGTTGAAC ACAACTGGTT CGAGACTTTC 
ATTGTCTTTA TGATTCTCCT CAGTAGTGGA GCTCTGGCTT TTGAAGACAT ATATATTGAA 
CAGCGTAAAA CTATCAAGAC TATGCTGGAA TATGCTGACA AGGTCTTCAC TTATATCTTC 
ATTCTGGAAA TGCTGCTGAA GTGGGTGGCC TATGGTTATC AGACATACTT TACTAATGCC 
TGGTGCTGGC TGGACTTCCT GATTGTCGAT GTCTCTTTGG TTAGCTTAAC AGCAAATGCA 
TTGGGTTACT CTGAACTTGG TGCCATTAAG TCCCTTAGGA CACTAAGAGC TTTGAGACCT 
CTAAGAGCTT TGTCACGATT TGAAGGCATG AGGGTGGTTG TGAATGCCCT TTTAGGAGCA 
ATTCCATCCA TCATGAACGT GCTTCTCGTT TGTCTTATAT TCTGGCTAAT TTTCAGCATC 
ATGGGAGTAA ATCTGTTTGC TGGGAAGTTC TACTACTGTG TTAACACCAC AACTGATGAA 
AGGTTTGAAG TCGACCAAAT CAACAATTTT AGTCAGTGCG AGGAGCTCAT AAAAAACAAT 
CAAAGTGCCC GGTGGAAAAA CGTGAAAGTA AACTTTGATA ATGTAGGATT TGGGTATCTG 
TCTTTACTTC AAGTAGCTAC ATTTAAAGGC TGGATGGATA TTATGTATGC AGCAATTGAC 
TCAAGAGATG TATTACAGCA ACCCAAGTAT GAAGACAACC TGTACATGTA TTTGTACTTT 
GTCATCTTTA TCATTTTTGG GTCTTTTTTC ACCCTGAACC TGTTCATTGG TGTCATTATT 
GATAACTTCA ATCAGCAAAA AAAGAAGTTT GGAGGTCAAG ACATATTTAT GACAGAAGAG 
CAGAAGAAAT ACTACAATGC AATGAAAAAG CTGGGATCCA AAAAACCACA AAAACCTATA 
CCAAGACCAG GGAACAAATT CCAAGGCATG GTCTTTGATT TTGTGACACA GCAAGTCTTT 
GACATCAGCA TCATGATTCT TATTTGTCTT AACATGGTGA CCATGATGGT AGAAACTGAT 
GACCAGAGTA AAGAAATGGA AAATATTTTA TACTGGATAA ACCTGGTGTT CATTGTCCTT 
TTTACTGGAG AATGTGTCCT CAAATTAATT TCACTTCGCC ATTACTATTT CACTATAGGC 
TGGAACATTT TTGATTTTGT GGTTGTCATT CTCTCAATAG TAGGTATGTT CTTAGCCGAG 
ATGATTGAGA AATATTTTGT CTCCCCCACG CTATTCCGAG TGATCCGACT CGCCAGAATC 
GGTCGAATCC TGCGTCTCAT TAAAGGCGCC AAGGGAATCC GCACTCTGCT TTTTGCTTTG 
ATGATGTCTC TTCCTGCTTT GTTCAACATT GGTCTGCTGC TCTTCCTGGT CATGTTTATC 
TATGCCATCT TTGGCATGTC CAACTTTGCC TACGTTAAGA GGGAAGCTGG GATTGATGAC 
ATGTTCAACT TTGAAACGTT TGGCAACAGC ATGATCTGCC TCTTTCAAAT CACCACATCT 
GCTGGCTGGG ATGGTTTGCT GGCCCCAATT CTTAACAGTG GGGAGCCAGA CTGTGACCCT 
AACAAAGCTC ATCCGGGGAG CTCTGTGAAA GGCGACTGTG GCAACCCTTC TGTTGGGATT 
TTCTTCTTTG TCAGCTACAT TATCATATCA TTTCTGGTAG TGGTGAACAT GTACATTGCT 
GTGATTTTGG AGAATTTTGG TGTTGCTACT GAAGAAAGTG CTGAACCCTT GAGCGAGGAT 
GACTTTGAGA TGTTCTACGA AGTCTGGGAG AAGTTTGATC CCGATGCAAC ACAATTCATG 
GAATTTGAGA AATTGTCTGA TTTTGCAGCA GCACTTGAGC CTCCTCTTCA TCTACCCAAA 
CCAAACAAAG TCCAGCTTAT TGCAATGGAT CTGCCCATGG TAAGCGGCGA CAGAATTCAC 
TGCCTTGACA TCTTGTTTGC TTTCACAAAG CGTGTTTTGG GGGAAAGTGG GGAGATGGAC 
GCCCTCAGAA TACAGATGGA AGATCGGTTT ATGGCATCCA ATCCTTCAAA AGCTTCCTAT 
GAACCAATTA CAACCACATT AAAAAGAAAA CAGGAGGAAG TATCTGCTGT CATTATTCAA 
CGGGCTTATA GGCGTCACCT TTTAAAACGA ACAGTAAAAC AAGCTTCCTT TATCTATAAC 
AAAAACAAAA CGGAAGGAGG GGCTGGTCAG GGTATGAAAG ATGAAATCCT TATTGACAAG 
TTAAATGAAA ACTCCTTTAC AGAGAAAACT GATATAACTG CATCAACAGC AGTTTGTCCA 
CCTTCTTATG ATCACGTAAC AAGGCCAGAC AAAAAAAAGC ATGAAAAGGA AGATAAAGGA 
GGTCAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_014954275.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014809761.1 Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X7, mRNA.

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CloneID	OCa74031
Clone ID Related Accession (Same CDS sequence)	XM_014954275.1
Accession Version	XM_014954275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sodium channel protein type 1 subunit alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGGC 
ACCACTACAG AAGCAGAAAT GAGGAAGAGA ATATCAGGCT CTTTCCACGT TTCTATGGAC 
TTCCTCGAAG ATCCAGCTTT AAGGGAAAGA GCCATGAGTA TTGCTAGCAT CCTCACAAAC 
ACTGTGGAAG AACTTGAAGA ATCAAGACAG AAATGCCCAC CATGTTGGTA TAAATTTGCA 
AATATTTTCT TGATCTGGGA CTGCAGTCCA CATTGGTTAA AAGTAAAACA TATTGTCAAC 
TTAGTGGTAA TGGACCCATT TGTTGACCTG GCTATTACAA TTTGCATTGT TTTAAATACA 
CTCTTTATGG CTATGGAACA TTATCCAATG ACCGATGAAT TCAATAACGT ACTTTCAGTT 
GGAAATCTGG TCTTCACTGG AATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCAATG 
GATCCTTACT ATTATTTCCA GGAGGGTTGG AATATTTTTG ATGGTTTTAT TGTAACCCTT 
AGCTTGGTTG AGCTTGGTCT TGCAGATGTG GAAGGACTCT CAGTTCTTCG ATCATTCAGA 
TTGTTACGAG TTTTCAAATT GGCAAAATCT TGGCCAACCC TAAATATGCT GATAAAAATT 
ATTGGTAATT CTGTGGGAGC TCTGGGGAAC CTGACCTTGG TGTTGGCCAT CATTGTGTTC 
ATTTTCGCTG TGGTTGGGAT GCAGCTGTTT GGGAAAAACT ATAAGGAATG TGTCTGCAAA 
ATTGCAAGTG ACTGTGTTCT TCCACGCTGG CATATGCAAG ATTTTTTTCA CTCTTTTCTG 
ATCGTATTTC GAGTGCTGTG TGGAGAATGG ATAGAAACGA TGTGGGACTG CATGGAGGTT 
GCTGGCCAAG CCATGTGCCT TACGGTCTTC ATGATGGTCA TGGTGATTGG AAACCTAGTG 
GTACTGAACC TATTTTTGGC CTTGCTCTTG AGCTCATTTA GTGCAGACAA CCTTGCCGTG 
ACAGATGATG ACAATGAAAT GAATAATCTC CAAATTGCTG TAGCAAGAAT GCAGAAGGGC 
ATAGATTATG TGAAGAGAAA AGCACGTGAA TTCATCCAAA AAGCTTTTGT TAAAAAACAA 
AAAGCTTTAG ATGAAATCAA ACCACTTGAA GATTTGAACA ACAAAAATAG CTGTATTTCT 
AACCATACAA CCGTGGATCT AAGCAAGGAC CATGACTATA TTAAAGATGT GAATGGAACA 
ACTAGTGGCA TAGGCACAGG TAGCAGTGTG GAAAAATACA TAATTGACGA AAGTGATTAC 
ATGTCATTCA TAAACAACCC AAGTCTCACT GTGACAGTGC CCATTGCTGT GGGTGAATCC 
GACTTTGAAA ATCTAAACAC AGAGGAATTT AGTAGTGAAT CAGACCTGGA AGAAAGCAAA 
GAGAAGTTGA ATTCGTCTAG TTCATCCGAA GGTAGCACAG TTGATATAGG ACTTCCTGCA 
GAAGAGCAAC CAGAAGCTGA ACCTGAAGAA GCCCAGGAGC CAGAGGCCTG CTTCACAGAA 
GGCTGTGTAC GAAGGTTCAA GTGCTGTCAA GTAAGTATAG AAGAAGGAAG AGGAAAGCAG 
TGGTGGAACC TTAGAAAAAC CTGCTTCAGG ATTGTTGAAC ACAACTGGTT CGAGACTTTC 
ATTGTCTTTA TGATTCTCCT CAGTAGTGGA GCTCTGGCTT TTGAAGACAT ATATATTGAA 
CAGCGTAAAA CTATCAAGAC TATGCTGGAA TATGCTGACA AGGTCTTCAC TTATATCTTC 
ATTCTGGAAA TGCTGCTGAA GTGGGTGGCC TATGGTTATC AGACATACTT TACTAATGCC 
TGGTGCTGGC TGGACTTCCT GATTGTCGAT GTCTCTTTGG TTAGCTTAAC AGCAAATGCA 
TTGGGTTACT CTGAACTTGG TGCCATTAAG TCCCTTAGGA CACTAAGAGC TTTGAGACCT 
CTAAGAGCTT TGTCACGATT TGAAGGCATG AGGGTGGTTG TGAATGCCCT TTTAGGAGCA 
ATTCCATCCA TCATGAACGT GCTTCTCGTT TGTCTTATAT TCTGGCTAAT TTTCAGCATC 
ATGGGAGTAA ATCTGTTTGC TGGGAAGTTC TACTACTGTG TTAACACCAC AACTGATGAA 
AGGTTTGAAG TCGACCAAAT CAACAATTTT AGTCAGTGCG AGGAGCTCAT AAAAAACAAT 
CAAAGTGCCC GGTGGAAAAA CGTGAAAGTA AACTTTGATA ATGTAGGATT TGGGTATCTG 
TCTTTACTTC AAGTAGCTAC ATTTAAAGGC TGGATGGATA TTATGTATGC AGCAATTGAC 
TCAAGAGATG TATTACAGCA ACCCAAGTAT GAAGACAACC TGTACATGTA TTTGTACTTT 
GTCATCTTTA TCATTTTTGG GTCTTTTTTC ACCCTGAACC TGTTCATTGG TGTCATTATT 
GATAACTTCA ATCAGCAAAA AAAGAAGTTT GGAGGTCAAG ACATATTTAT GACAGAAGAG 
CAGAAGAAAT ACTACAATGC AATGAAAAAG CTGGGATCCA AAAAACCACA AAAACCTATA 
CCAAGACCAG GGAACAAATT CCAAGGCATG GTCTTTGATT TTGTGACACA GCAAGTCTTT 
GACATCAGCA TCATGATTCT TATTTGTCTT AACATGGTGA CCATGATGGT AGAAACTGAT 
GACCAGAGTA AAGAAATGGA AAATATTTTA TACTGGATAA ACCTGGTGTT CATTGTCCTT 
TTTACTGGAG AATGTGTCCT CAAATTAATT TCACTTCGCC ATTACTATTT CACTATAGGC 
TGGAACATTT TTGATTTTGT GGTTGTCATT CTCTCAATAG TAGGTATGTT CTTAGCCGAG 
ATGATTGAGA AATATTTTGT CTCCCCCACG CTATTCCGAG TGATCCGACT CGCCAGAATC 
GGTCGAATCC TGCGTCTCAT TAAAGGCGCC AAGGGAATCC GCACTCTGCT TTTTGCTTTG 
ATGATGTCTC TTCCTGCTTT GTTCAACATT GGTCTGCTGC TCTTCCTGGT CATGTTTATC 
TATGCCATCT TTGGCATGTC CAACTTTGCC TACGTTAAGA GGGAAGCTGG GATTGATGAC 
ATGTTCAACT TTGAAACGTT TGGCAACAGC ATGATCTGCC TCTTTCAAAT CACCACATCT 
GCTGGCTGGG ATGGTTTGCT GGCCCCAATT CTTAACAGTG GGGAGCCAGA CTGTGACCCT 
AACAAAGCTC ATCCGGGGAG CTCTGTGAAA GGCGACTGTG GCAACCCTTC TGTTGGGATT 
TTCTTCTTTG TCAGCTACAT TATCATATCA TTTCTGGTAG TGGTGAACAT GTACATTGCT 
GTGATTTTGG AGAATTTTGG TGTTGCTACT GAAGAAAGTG CTGAACCCTT GAGCGAGGAT 
GACTTTGAGA TGTTCTACGA AGTCTGGGAG AAGTTTGATC CCGATGCAAC ACAATTCATG 
GAATTTGAGA AATTGTCTGA TTTTGCAGCA GCACTTGAGC CTCCTCTTCA TCTACCCAAA 
CCAAACAAAG TCCAGCTTAT TGCAATGGAT CTGCCCATGG TAAGCGGCGA CAGAATTCAC 
TGCCTTGACA TCTTGTTTGC TTTCACAAAG CGTGTTTTGG GGGAAAGTGG GGAGATGGAC 
GCCCTCAGAA TACAGATGGA AGATCGGTTT ATGGCATCCA ATCCTTCAAA AGCTTCCTAT 
GAACCAATTA CAACCACATT AAAAAGAAAA CAGGAGGAAG TATCTGCTGT CATTATTCAA 
CGGGCTTATA GGCGTCACCT TTTAAAACGA ACAGTAAAAC AAGCTTCCTT TATCTATAAC 
AAAAACAAAA CGGAAGGAGG GGCTGGTCAG GGTATGAAAG ATGAAATCCT TATTGACAAG 
TTAAATGAAA ACTCCTTTAC AGAGAAAACT GATATAACTG CATCAACAGC AGTTTGTCCA 
CCTTCTTATG ATCACGTAAC AAGGCCAGAC AAAAAAAAGC ATGAAAAGGA AGATAAAGGA 
GGTCAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014809761.1
CDS	55..6066
Translation	MEQSVLVPPGPDSFQYFTRESLAAIEQRIAAEKAKNSKQDRKDNDDENGPKPNSDLEAGKTLPFIYGDIPPGMVSEPLEDMDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPENFTLDTNITSQLNSTIGENGTLVNSTVTPFDWKGYIEDESHFYFLEGQNDALLCGNSSDAGQCPEGYTCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATMEEAEQKEAEFQQMLEQLKKQQEAAQAAAVAAAAVTASAESREPSAGGEAGGLSESSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFHKSESEDSIRRKGFRLSIEGNRLTYEKKYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDIGSENDFADDEHSTFEDNDSRRDSLFVPRRHGERRNSNISQASRSSRMLAVFPVNGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTEAEMRKRISGSFHVSMDFLEDPALRERAMSIASILTNTVEELEESRQKCPPCWYKFANIFLIWDCSPHWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDEFNNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYKECVCKIASDCVLPRWHMQDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVTDDDNEMNNLQIAVARMQKGIDYVKRKAREFIQKAFVKKQKALDEIKPLEDLNNKNSCISNHTTVDLSKDHDYIKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDLEESKEKLNSSSSSEGSTVDIGLPAEEQPEAEPEEAQEPEACFTEGCVRRFKCCQVSIEEGRGKQWWNLRKTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTDERFEVDQINNFSQCEELIKNNQSARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAIDSRDVLQQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSKEMENILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGEPDCDPNKAHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFGVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSDFAAALEPPLHLPKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEDRFMASNPSKASYEPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFIYNKNKTEGGAGQGMKDEILIDKLNENSFTEKTDITASTAVCPPSYDHVTRPDKKKHEKEDKGGQK

Target ORF information:

RefSeq Version	XM_014954275.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954275.1

ORF Insert Sequence:

ATGGAGCAGT CAGTGCTTGT ACCACCAGGA CCAGACAGCT TCCAATACTT CACTAGAGAG 
TCTCTTGCAG CTATTGAACA ACGCATTGCT GCAGAAAAAG CTAAGAATTC TAAACAAGAT 
CGTAAAGACA ACGATGATGA AAATGGGCCA AAGCCAAACA GTGATTTGGA GGCAGGAAAA 
ACACTTCCGT TTATATATGG CGATATACCT CCAGGAATGG TGTCTGAGCC CTTGGAAGAC 
ATGGACCCAT ACTACATCAA TAAAAAAACC TTTATAGTAT TGAATAAAGG GAAGGCAATC 
TTCCGGTTCA GTGCCACCTC TGCCCTGTAC ATTTTAACTC CCTTCAATCC TCTTAGAAAA 
ATAGCTATTA AAATTTTGGT ACATTCATTA TTCAGCATGC TTATCATGTG CACTATTTTG 
ACCAACTGTG TATTTATGAC CATGAGTAAC CCTCCGGACT GGACAAAGAA TGTAGAGTAT 
ACGTTCACTG GAATTTACAC GTTTGAGTCA CTCATAAAAA TCATTGCAAG AGGCTTTTGC 
TTAGAAGATT TTACTTTTCT TCGAGATCCA TGGAACTGGC TTGATTTCAC TGTTATTACC 
TTCGCGTATG TAACAGAATT TGTAAACCTA GGCAATGTTT CAGCTCTTCG AACTTTCAGA 
GTATTGAGAG CTTTGAAAAC TATTTCTGTA ATCCCAGGCT TGAAGACTAT CGTGGGAGCC 
CTAATTCAAT CTGTGAAGAA GCTCTCGGAT GTTATGATCC TGACCGTGTT TTGTCTGAGT 
GTATTTGCAC TAATAGGGCT GCAGCTGTTC ATGGGCAACT TGAGGAATAA ATGCCTGCAG 
TGGCCTCCAG AAAATTTTAC TCTGGATACA AATATTACTT CCCAGCTAAA CAGCACCATA 
GGTGAAAACG GTACATTGGT TAATTCAACA GTGACTCCAT TTGACTGGAA GGGGTACATT 
GAAGATGAAA GTCATTTTTA TTTTCTGGAA GGGCAAAACG ATGCTCTGCT TTGCGGAAAT 
AGTTCTGATG CTGGGCAGTG CCCAGAAGGA TATACATGTG TGAAAGCCGG TAGAAACCCC 
AATTATGGCT ACACAAGTTT TGACACCTTC AGCTGGGCTT TCTTGTCACT GTTTCGACTG 
ATGACTCAAG ACTTCTGGGA AAACCTTTAT CAATTGACAC TGCGCGCTGC TGGGAAGACA 
TACATGATAT TTTTTGTTCT GGTGATTTTC CTGGGTTCTT TCTACCTGAT CAACTTGATC 
TTGGCTGTGG TTGCCATGGC CTATGAAGAA CAGAATCAAG CAACTATGGA AGAAGCAGAA 
CAGAAAGAGG CAGAATTTCA ACAGATGCTG GAACAACTGA AGAAGCAACA AGAAGCAGCT 
CAGGCAGCAG CGGTGGCAGC AGCAGCAGTA ACAGCATCTG CGGAGTCAAG GGAGCCCAGC 
GCTGGGGGAG AAGCAGGAGG CCTCTCAGAA AGTTCCTCAG AGGCATCAAA ATTGAGCTCA 
AAGAGTGCAA AAGAGAGGAG AAACAGAAGG AAAAAAAGAA AACAGAAAGA ACAGTCTGGA 
GGAGAGGAAA AAGATGAAGA TGAATTTCAC AAGTCTGAAT CAGAAGACAG CATCAGAAGA 
AAAGGTTTCC GATTGTCAAT TGAAGGCAAT AGGTTGACAT ATGAAAAGAA GTATTCTTCT 
CCACATCAGT CTTTGTTGAG CATTCGAGGC TCCCTGTTTT CTCCGCGGCG TAACAGCAGA 
ACAAGTCTTT TCAGCTTCAG AGGTCGAGCA AAGGATATAG GATCAGAAAA TGACTTTGCT 
GATGATGAGC ACAGCACTTT TGAAGACAAT GATAGCAGAA GAGATTCTCT CTTTGTGCCG 
CGCAGACACG GTGAACGGCG CAACAGTAAC ATTAGTCAGG CCAGTAGGTC ATCCAGGATG 
CTGGCAGTGT TTCCAGTGAA TGGGAAGATG CACAGCACTG TGGATTGCAA TGGGGTGGTT 
TCCCTGGTTG GTGGACCATC TGTTCCTACA TCGCCTGTTG GACAGCTTCT GCCAGAGGGC 
ACCACTACAG AAGCAGAAAT GAGGAAGAGA ATATCAGGCT CTTTCCACGT TTCTATGGAC 
TTCCTCGAAG ATCCAGCTTT AAGGGAAAGA GCCATGAGTA TTGCTAGCAT CCTCACAAAC 
ACTGTGGAAG AACTTGAAGA ATCAAGACAG AAATGCCCAC CATGTTGGTA TAAATTTGCA 
AATATTTTCT TGATCTGGGA CTGCAGTCCA CATTGGTTAA AAGTAAAACA TATTGTCAAC 
TTAGTGGTAA TGGACCCATT TGTTGACCTG GCTATTACAA TTTGCATTGT TTTAAATACA 
CTCTTTATGG CTATGGAACA TTATCCAATG ACCGATGAAT TCAATAACGT ACTTTCAGTT 
GGAAATCTGG TCTTCACTGG AATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCAATG 
GATCCTTACT ATTATTTCCA GGAGGGTTGG AATATTTTTG ATGGTTTTAT TGTAACCCTT 
AGCTTGGTTG AGCTTGGTCT TGCAGATGTG GAAGGACTCT CAGTTCTTCG ATCATTCAGA 
TTGTTACGAG TTTTCAAATT GGCAAAATCT TGGCCAACCC TAAATATGCT GATAAAAATT 
ATTGGTAATT CTGTGGGAGC TCTGGGGAAC CTGACCTTGG TGTTGGCCAT CATTGTGTTC 
ATTTTCGCTG TGGTTGGGAT GCAGCTGTTT GGGAAAAACT ATAAGGAATG TGTCTGCAAA 
ATTGCAAGTG ACTGTGTTCT TCCACGCTGG CATATGCAAG ATTTTTTTCA CTCTTTTCTG 
ATCGTATTTC GAGTGCTGTG TGGAGAATGG ATAGAAACGA TGTGGGACTG CATGGAGGTT 
GCTGGCCAAG CCATGTGCCT TACGGTCTTC ATGATGGTCA TGGTGATTGG AAACCTAGTG 
GTACTGAACC TATTTTTGGC CTTGCTCTTG AGCTCATTTA GTGCAGACAA CCTTGCCGTG 
ACAGATGATG ACAATGAAAT GAATAATCTC CAAATTGCTG TAGCAAGAAT GCAGAAGGGC 
ATAGATTATG TGAAGAGAAA AGCACGTGAA TTCATCCAAA AAGCTTTTGT TAAAAAACAA 
AAAGCTTTAG ATGAAATCAA ACCACTTGAA GATTTGAACA ACAAAAATAG CTGTATTTCT 
AACCATACAA CCGTGGATCT AAGCAAGGAC CATGACTATA TTAAAGATGT GAATGGAACA 
ACTAGTGGCA TAGGCACAGG TAGCAGTGTG GAAAAATACA TAATTGACGA AAGTGATTAC 
ATGTCATTCA TAAACAACCC AAGTCTCACT GTGACAGTGC CCATTGCTGT GGGTGAATCC 
GACTTTGAAA ATCTAAACAC AGAGGAATTT AGTAGTGAAT CAGACCTGGA AGAAAGCAAA 
GAGAAGTTGA ATTCGTCTAG TTCATCCGAA GGTAGCACAG TTGATATAGG ACTTCCTGCA 
GAAGAGCAAC CAGAAGCTGA ACCTGAAGAA GCCCAGGAGC CAGAGGCCTG CTTCACAGAA 
GGCTGTGTAC GAAGGTTCAA GTGCTGTCAA GTAAGTATAG AAGAAGGAAG AGGAAAGCAG 
TGGTGGAACC TTAGAAAAAC CTGCTTCAGG ATTGTTGAAC ACAACTGGTT CGAGACTTTC 
ATTGTCTTTA TGATTCTCCT CAGTAGTGGA GCTCTGGCTT TTGAAGACAT ATATATTGAA 
CAGCGTAAAA CTATCAAGAC TATGCTGGAA TATGCTGACA AGGTCTTCAC TTATATCTTC 
ATTCTGGAAA TGCTGCTGAA GTGGGTGGCC TATGGTTATC AGACATACTT TACTAATGCC 
TGGTGCTGGC TGGACTTCCT GATTGTCGAT GTCTCTTTGG TTAGCTTAAC AGCAAATGCA 
TTGGGTTACT CTGAACTTGG TGCCATTAAG TCCCTTAGGA CACTAAGAGC TTTGAGACCT 
CTAAGAGCTT TGTCACGATT TGAAGGCATG AGGGTGGTTG TGAATGCCCT TTTAGGAGCA 
ATTCCATCCA TCATGAACGT GCTTCTCGTT TGTCTTATAT TCTGGCTAAT TTTCAGCATC 
ATGGGAGTAA ATCTGTTTGC TGGGAAGTTC TACTACTGTG TTAACACCAC AACTGATGAA 
AGGTTTGAAG TCGACCAAAT CAACAATTTT AGTCAGTGCG AGGAGCTCAT AAAAAACAAT 
CAAAGTGCCC GGTGGAAAAA CGTGAAAGTA AACTTTGATA ATGTAGGATT TGGGTATCTG 
TCTTTACTTC AAGTAGCTAC ATTTAAAGGC TGGATGGATA TTATGTATGC AGCAATTGAC 
TCAAGAGATG TATTACAGCA ACCCAAGTAT GAAGACAACC TGTACATGTA TTTGTACTTT 
GTCATCTTTA TCATTTTTGG GTCTTTTTTC ACCCTGAACC TGTTCATTGG TGTCATTATT 
GATAACTTCA ATCAGCAAAA AAAGAAGTTT GGAGGTCAAG ACATATTTAT GACAGAAGAG 
CAGAAGAAAT ACTACAATGC AATGAAAAAG CTGGGATCCA AAAAACCACA AAAACCTATA 
CCAAGACCAG GGAACAAATT CCAAGGCATG GTCTTTGATT TTGTGACACA GCAAGTCTTT 
GACATCAGCA TCATGATTCT TATTTGTCTT AACATGGTGA CCATGATGGT AGAAACTGAT 
GACCAGAGTA AAGAAATGGA AAATATTTTA TACTGGATAA ACCTGGTGTT CATTGTCCTT 
TTTACTGGAG AATGTGTCCT CAAATTAATT TCACTTCGCC ATTACTATTT CACTATAGGC 
TGGAACATTT TTGATTTTGT GGTTGTCATT CTCTCAATAG TAGGTATGTT CTTAGCCGAG 
ATGATTGAGA AATATTTTGT CTCCCCCACG CTATTCCGAG TGATCCGACT CGCCAGAATC 
GGTCGAATCC TGCGTCTCAT TAAAGGCGCC AAGGGAATCC GCACTCTGCT TTTTGCTTTG 
ATGATGTCTC TTCCTGCTTT GTTCAACATT GGTCTGCTGC TCTTCCTGGT CATGTTTATC 
TATGCCATCT TTGGCATGTC CAACTTTGCC TACGTTAAGA GGGAAGCTGG GATTGATGAC 
ATGTTCAACT TTGAAACGTT TGGCAACAGC ATGATCTGCC TCTTTCAAAT CACCACATCT 
GCTGGCTGGG ATGGTTTGCT GGCCCCAATT CTTAACAGTG GGGAGCCAGA CTGTGACCCT 
AACAAAGCTC ATCCGGGGAG CTCTGTGAAA GGCGACTGTG GCAACCCTTC TGTTGGGATT 
TTCTTCTTTG TCAGCTACAT TATCATATCA TTTCTGGTAG TGGTGAACAT GTACATTGCT 
GTGATTTTGG AGAATTTTGG TGTTGCTACT GAAGAAAGTG CTGAACCCTT GAGCGAGGAT 
GACTTTGAGA TGTTCTACGA AGTCTGGGAG AAGTTTGATC CCGATGCAAC ACAATTCATG 
GAATTTGAGA AATTGTCTGA TTTTGCAGCA GCACTTGAGC CTCCTCTTCA TCTACCCAAA 
CCAAACAAAG TCCAGCTTAT TGCAATGGAT CTGCCCATGG TAAGCGGCGA CAGAATTCAC 
TGCCTTGACA TCTTGTTTGC TTTCACAAAG CGTGTTTTGG GGGAAAGTGG GGAGATGGAC 
GCCCTCAGAA TACAGATGGA AGATCGGTTT ATGGCATCCA ATCCTTCAAA AGCTTCCTAT 
GAACCAATTA CAACCACATT AAAAAGAAAA CAGGAGGAAG TATCTGCTGT CATTATTCAA 
CGGGCTTATA GGCGTCACCT TTTAAAACGA ACAGTAAAAC AAGCTTCCTT TATCTATAAC 
AAAAACAAAA CGGAAGGAGG GGCTGGTCAG GGTATGAAAG ATGAAATCCT TATTGACAAG 
TTAAATGAAA ACTCCTTTAC AGAGAAAACT GATATAACTG CATCAACAGC AGTTTGTCCA 
CCTTCTTATG ATCACGTAAC AAGGCCAGAC AAAAAAAAGC ATGAAAAGGA AGATAAAGGA 
GGTCAGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SCN1A cDNA ORF clone, Calidris pugnax(ruff)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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