SSFA2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	SSFA2
Entrez Gene ID	106895261
Full Name	sperm specific antigen 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014954577.1	XP_014810063.1	sperm-specific antigen 2

Pathways from BioSystems
Gene Ontology

The following SSFA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSFA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa74238	XM_014954577.1 Latest version!	Calidris pugnax sperm specific antigen 2 (SSFA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSFA2 ( XM_014954577.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014810063.1 Calidris pugnax sperm specific antigen 2 (SSFA2), mRNA.

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CloneID	OCa74238
Clone ID Related Accession (Same CDS sequence)	XM_014954577.1
Accession Version	XM_014954577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	sperm-specific antigen 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090990.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGTGA GAAATGGAGG AAGTTTTGAA GATGACTTAT CCCTGGGAGC TGAAGCTAAT 
CATCTACGGA GTGATACCCA AATGGAAACA TGCAATGGTG TGCTGGCGAA GGAGAGGAGG 
CTGCAGTTCC ACCAGAAGGG GAGAAGTATG AACTCCTCCG GGTCTGGAAA AAGCAGTGCG 
ACTGTTTCAA GTGTTTCTGA ATTACTGGAG CTTTATGAGG AAGATCCTGA AGAAGTGCTC 
TATAACCTTG GGTTTGGAAG AGATGAACCA GATATTGCTT CAAAAATTCC AGCGAGATTT 
TTTAATTCAT CGTCATTTGC TAGAGGAATA GATATCAAAG TGTTTTTGAA TGCCCAAATA 
CAGCGCATGG AAGTGGAAAA TCCAAATTTT GCTTTAACAA GTCGTTTTCG TCAGATTGAA 
GTGCTTACTA CTGTGGCCAA TGCTTTTTCT TCTTTGTATT CTCAAGTCTC TGGGACTCCT 
TTGCAGAAAA TTGGTAGTAT GAATTCAGTG TCTTCCAGCA AAGAGGCGTC CAGCCCTCCC 
CCTTTAAATC GCAGCAATAC AGCCAGTCGG TTAATGAAGA CCTTGTCAAA GCTCAATCTG 
TGCGGCACAC AGCAAGCCGA CGGTAGCGGC TGCTCGAGTC CTTCACCCGT TGAAAAAGGG 
AAAAGTCCAG CTGAACTGGG GAGCGAGGAA CAGGATGTTA AAAATGAATC TAAATTACAA 
AAACACTCTA AAAAGAAAGA CTCTCCCTCT TTTTTGGCTA CTGTAAAGGA GGAAATAGCC 
GGTAGTCAAA CAAGTGCTGC GCATAATCCT GCAAACCTTC CTGCTGGGAC TGATGATAAA 
CGAGAGGGTA CTGTGCCTCC AGCAGACGTG CAAAGCAGCA GGTTGGCAAG TGCCCAGGAT 
AGACCGTGTG AAGAATCTGG TCTTTTGGAC TCACCAAGCC TCAGCAGCAG CTCCAATACA 
TCTAACAGCA GCACCTCGAC GCAAAGGCTG GTTGTGGCAT CGCCAGGTAG AGACCCCTGT 
GCTCCTCTTG CAAACCCCTC CATAATGAAT CTAATGCTGC AGCAAAAGGA CTCCTTTGAG 
ATGGAAGAGA TCCAGAGTAC AGAGGGGGAT CTACCGCATG TTCCAGGTTC TCACCAGCTG 
GCAGTGACCA AGTCAAGGAA AGATCAGCTG TTACGAACAG CTAGTCAGCA CTCTGACAGC 
AGTGGTTTTG CTGAGGACTC CTCCACAGAT TGTTCTCCAT TTAATCAGCT TCAGGTTCAG 
GAGTCTTTGC AGGGCATGGG AAGCAGTGCT GACAGCTGTG ACAGTGAGAC AACTGTGACG 
TCGCATAGCG AGGAACAGAA GACACCAATA GCCATGGAAC TGCCCTCTTT CAATAAGTTT 
GAGGAGGAAG AGGAAGAAGA TGATGATGAT GACGACGATG ATGATGATGA AGAGGATATT 
ATCTCTCAAG TAGAGAGACA AAAGGTAGGA GCACCTTCTG AGAGCAGGAG TGAAGACAGA 
AAAATTGTTG ACTATGAAAC TGAACAAAAT CAAGGAGGAG AAAGCAAGTC ATCTCATGTA 
TTAGAGACAG TCCAAGGAGA GGTCAGCTCT AAAGAGGAAG ACACTTCCAG TGAGCAAAAA 
CAATTGGACC TGCTGGAGGA AGGCAGTGTG TTTGAGTTTC CTCAGTACCA CACTCACCAT 
ATCCTCAAGT CCATGGAGTC TGTGGAAGGT GGCAGCTCCT CCCCGTCATC TGTGGATAGG 
GTTCACGTTG CCTTGCAAAG AGCCGAGATG AGGATCATCA GAACATCTGA TTGCAGGGCT 
GGACGCAGTC TGCTTAAGTC AAAAGATCTC CTGAGGAAGC AGAGGCTCTG GTTTGCTGAA 
TCGGGCTATC CTCTAAGACG ATCTCAGTCT CTCCCAACTC CGTTGCTGAA TCCAGTGAAA 
GTGGTGTCCT CTGTGAATGT CCAGTTAACT CCAGGAAAAG AGACTCTATG CAGTCCTCCT 
TCTTTCACCT ACAAGTACAC ACGTGTGGAG GAAGAGGAGA AACCAACGTC TGAGAGTGAC 
AAAATTGAGG GTGAGGCAGT CACCAACCAG CCAGTGTGCA AATCCACACT TTTCATTGCA 
CCTTCGTCCT CCAAAAAAGA AGTGCCCCAG ACTGGGCCCA ATAGACTGTC AGAGGAGTCC 
AGTCCCACCA GGGTACAGAG CTGCTCACTG CACACGCCAG CACACATGTC CCAGTCGACG 
TGCTCCCTCC ACTCCCTCCC TGCCGAGTGG CAGGACAGAC CATTCTGCGA GCACATGAGG 
ACACTGAGCA CCCACAGCAT CCCAAGCCTC TCTGGCTCTT CCTTTGGTGC CTTGCTTTCC 
CCCTTCAGCT GTCCCTTCTC TCCAAGGCAT GCTGGATTTC CTCATCGACC TCATGCCATC 
AACCCGCCCT CCACTGTGGA GATGCAGCTG CGCAGGGTCC TGCACGACAT CAGAAACTCT 
CTGCAGAACC TCTCACAGTA CCCGGTGATG AGGGGTCAGG ATCTTTCAGC GGCCACTGGT 
TACACTACTC ACAGATCATC CATTCTACCT CTCTACGAAA ATACTTTCCA GGAGCTACAA 
GTGGTAAGGA GAAATCTAAA CTTATTCAGA ACCCAAATGA TGGATTTAGA ATTGACTATG 
TTACGTCAAC AGACAACAGT TTATCAGCAC ATGACAGAAG AGGAAAGATA TGAAGCTGAT 
CAGCTACAGA GCCTGAGAAA GTCAGTCCGC ATGGAGCTAC AGGAGTTAGA GATGCAGTTA 
GAAGAACGGC TACTTGCTTT GGAAGACCAG CTGAGAAGTC TGCACTTGTC TTCACCCTAC 
AGACCTCAGA CACACATAGG TATGTATGGA AGTAGAAGTG CTGATAATCT ATCATGCCCT 
TCTCCATTGA ATGTCATTGA ACCAGTCTCT GAACTTATTC GTGAGCAGTC ATTTCTGAAG 
TCTGAACTGG GTTTAGGAGT GGGAGATCTT GGACTGGAAA CTCTCCCAGC AGAGGGTACG 
GAGTCCGTAT TCTCCCATGG AAACTCTGAT TCCTCTTCAG TGTGCTCTGG TCACGCAGGT 
AGAAAAGCTG GGTTTGGATC CTCTGAACTA ACAGGAAGGT CTAAGAGTCA AAGCAAGAAG 
TTATACCGAG CCTCCGTTGC CTTAACACCA ACGCCTCCAG TGAGAGCAGT CACTGGACAG 
CAGGTGGAAA GCTCCGAGGA GCTTGCAGAC TCCAAGCCAG AGGTGGAACA CAAACTTGAA 
AAAGTCCCTC TCGTGCCTTC GGTTGATGAA ATCCATAAAA GCGTGGAGCA GGAGGAGCTG 
CAGCAAGTCA TACGGGAGAT CAAGGAATCT ATTGTTGGTG AAATAAGACG AGAAATAGTA 
AGTGGATTGT TAGCAGCTGT ATCACCCCAA AGCAGATCTG CAACTGCAAA ACAAGATACA 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014810063.1
CDS	138..3566
Translation	MLVRNGGSFEDDLSLGAEANHLRSDTQMETCNGVLAKERRLQFHQKGRSMNSSGSGKSSATVSSVSELLELYEEDPEEVLYNLGFGRDEPDIASKIPARFFNSSSFARGIDIKVFLNAQIQRMEVENPNFALTSRFRQIEVLTTVANAFSSLYSQVSGTPLQKIGSMNSVSSSKEASSPPPLNRSNTASRLMKTLSKLNLCGTQQADGSGCSSPSPVEKGKSPAELGSEEQDVKNESKLQKHSKKKDSPSFLATVKEEIAGSQTSAAHNPANLPAGTDDKREGTVPPADVQSSRLASAQDRPCEESGLLDSPSLSSSSNTSNSSTSTQRLVVASPGRDPCAPLANPSIMNLMLQQKDSFEMEEIQSTEGDLPHVPGSHQLAVTKSRKDQLLRTASQHSDSSGFAEDSSTDCSPFNQLQVQESLQGMGSSADSCDSETTVTSHSEEQKTPIAMELPSFNKFEEEEEEDDDDDDDDDDEEDIISQVERQKVGAPSESRSEDRKIVDYETEQNQGGESKSSHVLETVQGEVSSKEEDTSSEQKQLDLLEEGSVFEFPQYHTHHILKSMESVEGGSSSPSSVDRVHVALQRAEMRIIRTSDCRAGRSLLKSKDLLRKQRLWFAESGYPLRRSQSLPTPLLNPVKVVSSVNVQLTPGKETLCSPPSFTYKYTRVEEEEKPTSESDKIEGEAVTNQPVCKSTLFIAPSSSKKEVPQTGPNRLSEESSPTRVQSCSLHTPAHMSQSTCSLHSLPAEWQDRPFCEHMRTLSTHSIPSLSGSSFGALLSPFSCPFSPRHAGFPHRPHAINPPSTVEMQLRRVLHDIRNSLQNLSQYPVMRGQDLSAATGYTTHRSSILPLYENTFQELQVVRRNLNLFRTQMMDLELTMLRQQTTVYQHMTEEERYEADQLQSLRKSVRMELQELEMQLEERLLALEDQLRSLHLSSPYRPQTHIGMYGSRSADNLSCPSPLNVIEPVSELIREQSFLKSELGLGVGDLGLETLPAEGTESVFSHGNSDSSSVCSGHAGRKAGFGSSELTGRSKSQSKKLYRASVALTPTPPVRAVTGQQVESSEELADSKPEVEHKLEKVPLVPSVDEIHKSVEQEELQQVIREIKESIVGEIRREIVSGLLAAVSPQSRSATAKQDTQP

Target ORF information:

RefSeq Version	XM_014954577.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax sperm specific antigen 2 (SSFA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014954577.1

ORF Insert Sequence:

ATGCTGGTGA GAAATGGAGG AAGTTTTGAA GATGACTTAT CCCTGGGAGC TGAAGCTAAT 
CATCTACGGA GTGATACCCA AATGGAAACA TGCAATGGTG TGCTGGCGAA GGAGAGGAGG 
CTGCAGTTCC ACCAGAAGGG GAGAAGTATG AACTCCTCCG GGTCTGGAAA AAGCAGTGCG 
ACTGTTTCAA GTGTTTCTGA ATTACTGGAG CTTTATGAGG AAGATCCTGA AGAAGTGCTC 
TATAACCTTG GGTTTGGAAG AGATGAACCA GATATTGCTT CAAAAATTCC AGCGAGATTT 
TTTAATTCAT CGTCATTTGC TAGAGGAATA GATATCAAAG TGTTTTTGAA TGCCCAAATA 
CAGCGCATGG AAGTGGAAAA TCCAAATTTT GCTTTAACAA GTCGTTTTCG TCAGATTGAA 
GTGCTTACTA CTGTGGCCAA TGCTTTTTCT TCTTTGTATT CTCAAGTCTC TGGGACTCCT 
TTGCAGAAAA TTGGTAGTAT GAATTCAGTG TCTTCCAGCA AAGAGGCGTC CAGCCCTCCC 
CCTTTAAATC GCAGCAATAC AGCCAGTCGG TTAATGAAGA CCTTGTCAAA GCTCAATCTG 
TGCGGCACAC AGCAAGCCGA CGGTAGCGGC TGCTCGAGTC CTTCACCCGT TGAAAAAGGG 
AAAAGTCCAG CTGAACTGGG GAGCGAGGAA CAGGATGTTA AAAATGAATC TAAATTACAA 
AAACACTCTA AAAAGAAAGA CTCTCCCTCT TTTTTGGCTA CTGTAAAGGA GGAAATAGCC 
GGTAGTCAAA CAAGTGCTGC GCATAATCCT GCAAACCTTC CTGCTGGGAC TGATGATAAA 
CGAGAGGGTA CTGTGCCTCC AGCAGACGTG CAAAGCAGCA GGTTGGCAAG TGCCCAGGAT 
AGACCGTGTG AAGAATCTGG TCTTTTGGAC TCACCAAGCC TCAGCAGCAG CTCCAATACA 
TCTAACAGCA GCACCTCGAC GCAAAGGCTG GTTGTGGCAT CGCCAGGTAG AGACCCCTGT 
GCTCCTCTTG CAAACCCCTC CATAATGAAT CTAATGCTGC AGCAAAAGGA CTCCTTTGAG 
ATGGAAGAGA TCCAGAGTAC AGAGGGGGAT CTACCGCATG TTCCAGGTTC TCACCAGCTG 
GCAGTGACCA AGTCAAGGAA AGATCAGCTG TTACGAACAG CTAGTCAGCA CTCTGACAGC 
AGTGGTTTTG CTGAGGACTC CTCCACAGAT TGTTCTCCAT TTAATCAGCT TCAGGTTCAG 
GAGTCTTTGC AGGGCATGGG AAGCAGTGCT GACAGCTGTG ACAGTGAGAC AACTGTGACG 
TCGCATAGCG AGGAACAGAA GACACCAATA GCCATGGAAC TGCCCTCTTT CAATAAGTTT 
GAGGAGGAAG AGGAAGAAGA TGATGATGAT GACGACGATG ATGATGATGA AGAGGATATT 
ATCTCTCAAG TAGAGAGACA AAAGGTAGGA GCACCTTCTG AGAGCAGGAG TGAAGACAGA 
AAAATTGTTG ACTATGAAAC TGAACAAAAT CAAGGAGGAG AAAGCAAGTC ATCTCATGTA 
TTAGAGACAG TCCAAGGAGA GGTCAGCTCT AAAGAGGAAG ACACTTCCAG TGAGCAAAAA 
CAATTGGACC TGCTGGAGGA AGGCAGTGTG TTTGAGTTTC CTCAGTACCA CACTCACCAT 
ATCCTCAAGT CCATGGAGTC TGTGGAAGGT GGCAGCTCCT CCCCGTCATC TGTGGATAGG 
GTTCACGTTG CCTTGCAAAG AGCCGAGATG AGGATCATCA GAACATCTGA TTGCAGGGCT 
GGACGCAGTC TGCTTAAGTC AAAAGATCTC CTGAGGAAGC AGAGGCTCTG GTTTGCTGAA 
TCGGGCTATC CTCTAAGACG ATCTCAGTCT CTCCCAACTC CGTTGCTGAA TCCAGTGAAA 
GTGGTGTCCT CTGTGAATGT CCAGTTAACT CCAGGAAAAG AGACTCTATG CAGTCCTCCT 
TCTTTCACCT ACAAGTACAC ACGTGTGGAG GAAGAGGAGA AACCAACGTC TGAGAGTGAC 
AAAATTGAGG GTGAGGCAGT CACCAACCAG CCAGTGTGCA AATCCACACT TTTCATTGCA 
CCTTCGTCCT CCAAAAAAGA AGTGCCCCAG ACTGGGCCCA ATAGACTGTC AGAGGAGTCC 
AGTCCCACCA GGGTACAGAG CTGCTCACTG CACACGCCAG CACACATGTC CCAGTCGACG 
TGCTCCCTCC ACTCCCTCCC TGCCGAGTGG CAGGACAGAC CATTCTGCGA GCACATGAGG 
ACACTGAGCA CCCACAGCAT CCCAAGCCTC TCTGGCTCTT CCTTTGGTGC CTTGCTTTCC 
CCCTTCAGCT GTCCCTTCTC TCCAAGGCAT GCTGGATTTC CTCATCGACC TCATGCCATC 
AACCCGCCCT CCACTGTGGA GATGCAGCTG CGCAGGGTCC TGCACGACAT CAGAAACTCT 
CTGCAGAACC TCTCACAGTA CCCGGTGATG AGGGGTCAGG ATCTTTCAGC GGCCACTGGT 
TACACTACTC ACAGATCATC CATTCTACCT CTCTACGAAA ATACTTTCCA GGAGCTACAA 
GTGGTAAGGA GAAATCTAAA CTTATTCAGA ACCCAAATGA TGGATTTAGA ATTGACTATG 
TTACGTCAAC AGACAACAGT TTATCAGCAC ATGACAGAAG AGGAAAGATA TGAAGCTGAT 
CAGCTACAGA GCCTGAGAAA GTCAGTCCGC ATGGAGCTAC AGGAGTTAGA GATGCAGTTA 
GAAGAACGGC TACTTGCTTT GGAAGACCAG CTGAGAAGTC TGCACTTGTC TTCACCCTAC 
AGACCTCAGA CACACATAGG TATGTATGGA AGTAGAAGTG CTGATAATCT ATCATGCCCT 
TCTCCATTGA ATGTCATTGA ACCAGTCTCT GAACTTATTC GTGAGCAGTC ATTTCTGAAG 
TCTGAACTGG GTTTAGGAGT GGGAGATCTT GGACTGGAAA CTCTCCCAGC AGAGGGTACG 
GAGTCCGTAT TCTCCCATGG AAACTCTGAT TCCTCTTCAG TGTGCTCTGG TCACGCAGGT 
AGAAAAGCTG GGTTTGGATC CTCTGAACTA ACAGGAAGGT CTAAGAGTCA AAGCAAGAAG 
TTATACCGAG CCTCCGTTGC CTTAACACCA ACGCCTCCAG TGAGAGCAGT CACTGGACAG 
CAGGTGGAAA GCTCCGAGGA GCTTGCAGAC TCCAAGCCAG AGGTGGAACA CAAACTTGAA 
AAAGTCCCTC TCGTGCCTTC GGTTGATGAA ATCCATAAAA GCGTGGAGCA GGAGGAGCTG 
CAGCAAGTCA TACGGGAGAT CAAGGAATCT ATTGTTGGTG AAATAAGACG AGAAATAGTA 
AGTGGATTGT TAGCAGCTGT ATCACCCCAA AGCAGATCTG CAACTGCAAA ACAAGATACA 
CAGCCCTGA