DDX1 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX1
Entrez Gene ID	106896699
Full Name	DEAD-box helicase 1
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014956880.1	XP_014812366.1	ATP-dependent RNA helicase DDX1

Pathways from BioSystems
Gene Ontology

The following DDX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa75946	XM_014956880.1 Latest version!	Calidris pugnax DEAD (Asp-Glu-Ala-Asp) box helicase 1 (DDX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX1 ( XM_014956880.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014812366.1 Calidris pugnax DEAD (Asp-Glu-Ala-Asp) box helicase 1 (DDX1), mRNA.

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CloneID	OCa75946
Clone ID Related Accession (Same CDS sequence)	XM_014956880.1
Accession Version	XM_014956880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	ATP-dependent RNA helicase DDX1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015091064.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT TTTCGGAAAT GGGTGTTATG CCTGAGATAG CTCAAGCAGT GGAGGAGATG 
GACTGGCTTC TTCCTACAGA TATCCAAGCA GAATCCATCC CACTTATCCT GGGAGGTGGT 
GATGTACTTA TGGCTGCTGA AACAGGGAGT GGAAAAACTG GTGCTTTTAG CATTCCAGTT 
ATCCAGATTG TTTATGAAAC GCTGAAGGAC CAGATGGAAG GCAAGAAAGG GAAAGCAACG 
ATTAAAACCG GTGGGGCAGT GCTGAATAAA TGGCAAATGA ACCCATATGA TAGAGGAACA 
GCTTTTGCGA TTGGATCAGA CGGTTTGTGT TGTCAAAGCA GAGAGATAAA AGAATGGCAT 
GGATGCAGAG CTACTAGAGG CGTGACTAAA GGGAAATACT ACTATGAAGT TTCCTGTCAT 
GACCAAGGCT TGTGCAGAGT TGGTTGGTCA ACTATGCAGG CTTCGCTAGA TTTGGGCACT 
GATAAATTTG GCTTTGGCTT TGGTGGCACT GGTAAGAAGT CTCACAACAA GCAATTTGAC 
AGCTACGGTG AGGAATTCAC TATGCATGAT ACAATTGGGT GTTATCTAGA CCTAGATAAA 
GGACAAATAA AATTTTCCAA AAATGGGAAG GACCTTGGCC TTGCGTTTGA AATCCCACCG 
CACATAAGGA ACCAAGCACT TTTTGCAGCT TGTGTACTGA AGAATGCTGA ATTAAAATTC 
AATTTTGGTG AAGAAGACTT CAAGTATCCA CCAAAAGATG GCTATGTTGG ACTTTGCAAG 
GCGCCTGATG GTAGTGTTGT AAAATCACAA CACTCAGGAA ATGCACAAGT GGTTCAAACA 
CAGAATCTTC CAAATGCTCC AAAAGCATTG ATTGTAGAAC CCTCAAGAGA ACTGGCAGAA 
CAAACTTTGA ACAATGTGAA GCAGTTCAAA AAATACGTTG ATAATCCCAA ATTAAGGGAA 
CTGTTGATTA TTGGTGGTGT TGCTGCAAGA GATCAACTCT CTGTACTGGA ACAAGGAGTG 
GATATTGTTG TTGGAACTCC TGGAAGACTG GATGACCTGG TCTCAACGGG AAAGCTTAAT 
TTATCTCAAG TTAGATTCCT GGTTCTGGAT GAGGCTGATG GCCTTCTCCT CCAAGGTTAT 
TCAGACTTCA TAAACAGGAT CCATAGTCAA ATTCCTCAGA TAACTTCAGA CGGAAAGAGA 
CTTCAGGTGA TTGTGTGCTC TGCAACGCTG CATTCTTTTG ATGTGAAAAA ACTATCTGAA 
AAGATCATGC ATTTTCCCAC CTGGGTTGAT TTGAAAGGAG AAGATTCTGT CCCTGAAACT 
GTACACCACG TAGTTGTGCC TGTAAATCCA AAGGCTGACA AACTCTGGGA AAGGCTCGGC 
AAGAATCATA TCAGAACTGA TGAAGTACAT GCAAAAGACA ACACACGACC TGGCGCTAAC 
ACTCCAGAAA TGTGGTCAGA AGCTATTAAA ATTCTAAAAG GAGAATACAC TGTTCGGGCA 
ATCAAAGAAC ACAAGATGGA CCAAGCCATT ATTTTCTGCA GGACTAAAAT TGATTGTGAT 
AATATGGAGC AGTACTTCAT CCAACAGGGC GGAGGCCCTG ACAGGAAGGG ACATCAGTTC 
TCATGTGTCT GTCTGCATGG TGACAGAAAA CCTCAAGAAA GAAAGCAAAA CCTGGAAAGA 
TTTAAGAGGG GAGATGTCCG TTTCTTGATC TGCACAGATG TTGCTGCCAG AGGAATTGAT 
ATTCATGGTG TTCCGTATGT TATCAATGTC ACACTCCCTG ATGAAAAGCA GAACTACGTT 
CATCGAATTG GGAGAGTAGG AAGAGCTGAG AGGATGGGTC TGGCAATCTC CCTTGTGGCA 
AAAGAGAAAG AAAAGGTTTG GTATCATGTG TGCAGTAGCC GTGGAAAAGG GTGTTACAAT 
ACTCGACTGA AGGAGGAAGG AGGTTGCACC ATCTGGTACA ATGAGATGCA GTTGCTGGGA 
GAGATTGAAG AACACTTAAA CTGCACTATT GCCCAAGTTG AACCAGACAT AAAAGTACCA 
GTAGATGATT TTGATGGGAA AGTTACATAT GGGCAGAAAA GAGCTCTGGG TGGTGGACTC 
TATAAAGGCC ATGTGGATAT TCTGGCACCT ACAGTACAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCTCAGA CATCTTTCTT GCATCTTGGC TATCTTCCTA ACCAGCTGTT CAGAACATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014812366.1
CDS	83..2305
Translation	MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQMEGKKGKATIKTGGAVLNKWQMNPYDRGTAFAIGSDGLCCQSREIKEWHGCRATRGVTKGKYYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDSYGEEFTMHDTIGCYLDLDKGQIKFSKNGKDLGLAFEIPPHIRNQALFAACVLKNAELKFNFGEEDFKYPPKDGYVGLCKAPDGSVVKSQHSGNAQVVQTQNLPNAPKALIVEPSRELAEQTLNNVKQFKKYVDNPKLRELLIIGGVAARDQLSVLEQGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLLQGYSDFINRIHSQIPQITSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPETVHHVVVPVNPKADKLWERLGKNHIRTDEVHAKDNTRPGANTPEMWSEAIKILKGEYTVRAIKEHKMDQAIIFCRTKIDCDNMEQYFIQQGGGPDRKGHQFSCVCLHGDRKPQERKQNLERFKRGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVAKEKEKVWYHVCSSRGKGCYNTRLKEEGGCTIWYNEMQLLGEIEEHLNCTIAQVEPDIKVPVDDFDGKVTYGQKRALGGGLYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

Target ORF information:

RefSeq Version	XM_014956880.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax DEAD (Asp-Glu-Ala-Asp) box helicase 1 (DDX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014956880.1

ORF Insert Sequence:

ATGGCGGCGT TTTCGGAAAT GGGTGTTATG CCTGAGATAG CTCAAGCAGT GGAGGAGATG 
GACTGGCTTC TTCCTACAGA TATCCAAGCA GAATCCATCC CACTTATCCT GGGAGGTGGT 
GATGTACTTA TGGCTGCTGA AACAGGGAGT GGAAAAACTG GTGCTTTTAG CATTCCAGTT 
ATCCAGATTG TTTATGAAAC GCTGAAGGAC CAGATGGAAG GCAAGAAAGG GAAAGCAACG 
ATTAAAACCG GTGGGGCAGT GCTGAATAAA TGGCAAATGA ACCCATATGA TAGAGGAACA 
GCTTTTGCGA TTGGATCAGA CGGTTTGTGT TGTCAAAGCA GAGAGATAAA AGAATGGCAT 
GGATGCAGAG CTACTAGAGG CGTGACTAAA GGGAAATACT ACTATGAAGT TTCCTGTCAT 
GACCAAGGCT TGTGCAGAGT TGGTTGGTCA ACTATGCAGG CTTCGCTAGA TTTGGGCACT 
GATAAATTTG GCTTTGGCTT TGGTGGCACT GGTAAGAAGT CTCACAACAA GCAATTTGAC 
AGCTACGGTG AGGAATTCAC TATGCATGAT ACAATTGGGT GTTATCTAGA CCTAGATAAA 
GGACAAATAA AATTTTCCAA AAATGGGAAG GACCTTGGCC TTGCGTTTGA AATCCCACCG 
CACATAAGGA ACCAAGCACT TTTTGCAGCT TGTGTACTGA AGAATGCTGA ATTAAAATTC 
AATTTTGGTG AAGAAGACTT CAAGTATCCA CCAAAAGATG GCTATGTTGG ACTTTGCAAG 
GCGCCTGATG GTAGTGTTGT AAAATCACAA CACTCAGGAA ATGCACAAGT GGTTCAAACA 
CAGAATCTTC CAAATGCTCC AAAAGCATTG ATTGTAGAAC CCTCAAGAGA ACTGGCAGAA 
CAAACTTTGA ACAATGTGAA GCAGTTCAAA AAATACGTTG ATAATCCCAA ATTAAGGGAA 
CTGTTGATTA TTGGTGGTGT TGCTGCAAGA GATCAACTCT CTGTACTGGA ACAAGGAGTG 
GATATTGTTG TTGGAACTCC TGGAAGACTG GATGACCTGG TCTCAACGGG AAAGCTTAAT 
TTATCTCAAG TTAGATTCCT GGTTCTGGAT GAGGCTGATG GCCTTCTCCT CCAAGGTTAT 
TCAGACTTCA TAAACAGGAT CCATAGTCAA ATTCCTCAGA TAACTTCAGA CGGAAAGAGA 
CTTCAGGTGA TTGTGTGCTC TGCAACGCTG CATTCTTTTG ATGTGAAAAA ACTATCTGAA 
AAGATCATGC ATTTTCCCAC CTGGGTTGAT TTGAAAGGAG AAGATTCTGT CCCTGAAACT 
GTACACCACG TAGTTGTGCC TGTAAATCCA AAGGCTGACA AACTCTGGGA AAGGCTCGGC 
AAGAATCATA TCAGAACTGA TGAAGTACAT GCAAAAGACA ACACACGACC TGGCGCTAAC 
ACTCCAGAAA TGTGGTCAGA AGCTATTAAA ATTCTAAAAG GAGAATACAC TGTTCGGGCA 
ATCAAAGAAC ACAAGATGGA CCAAGCCATT ATTTTCTGCA GGACTAAAAT TGATTGTGAT 
AATATGGAGC AGTACTTCAT CCAACAGGGC GGAGGCCCTG ACAGGAAGGG ACATCAGTTC 
TCATGTGTCT GTCTGCATGG TGACAGAAAA CCTCAAGAAA GAAAGCAAAA CCTGGAAAGA 
TTTAAGAGGG GAGATGTCCG TTTCTTGATC TGCACAGATG TTGCTGCCAG AGGAATTGAT 
ATTCATGGTG TTCCGTATGT TATCAATGTC ACACTCCCTG ATGAAAAGCA GAACTACGTT 
CATCGAATTG GGAGAGTAGG AAGAGCTGAG AGGATGGGTC TGGCAATCTC CCTTGTGGCA 
AAAGAGAAAG AAAAGGTTTG GTATCATGTG TGCAGTAGCC GTGGAAAAGG GTGTTACAAT 
ACTCGACTGA AGGAGGAAGG AGGTTGCACC ATCTGGTACA ATGAGATGCA GTTGCTGGGA 
GAGATTGAAG AACACTTAAA CTGCACTATT GCCCAAGTTG AACCAGACAT AAAAGTACCA 
GTAGATGATT TTGATGGGAA AGTTACATAT GGGCAGAAAA GAGCTCTGGG TGGTGGACTC 
TATAAAGGCC ATGTGGATAT TCTGGCACCT ACAGTACAAG AGTTGGCTGC CCTTGAAAAG 
GAGGCTCAGA CATCTTTCTT GCATCTTGGC TATCTTCCTA ACCAGCTGTT CAGAACATTC 
TGA