GCG cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCG
Entrez Gene ID	403571
Full Name	glucagon
Synonyms	GLP-1
General protein information	Preferred Names glucagon Names glucagon preproglucagon
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022414644.1	XP_022270352.1	glucagon isoform X1
NM_001003044.1	NP_001003044.1	glucagon precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gcg	NP_032126.1
Danio rerio (zebrafish)	gcga	NP_001258699.1
Pan troglodytes (chimpanzee)	GCG	XP_003309324.1
	GCG	XP_001093871.1
Gallus gallus (chicken)	GCG	NP_001177094.1
Xenopus tropicalis (tropical clawed frog)	gcg	NP_001006913.1
Canis lupus familiaris (dog)	GCG	NP_001003044.1
Bos taurus (cattle)	GCG	NP_776341.1
Rattus norvegicus (Norway rat)	Gcg	NP_036839.2

Gene Ontology
Bibliography

Related articles in PubMed

Involvement of glucagon-like peptide 1 in the glucose homeostasis regulation in obese and pituitary-dependent hyperadrenocorticism affected dogs.
Miceli DD, Cabrera Blatter MF, Gallelli MF, Pignataro OP, Castillo VA
Research in veterinary science97(2)211-7(2014 Oct)

Synergistic effect of portal glucose and glucagon-like peptide-1 to lower systemic glucose and stimulate counter-regulatory hormones.
Ionut V, Hucking K, Liberty IF, Bergman RN
Diabetologia48(5)967-75(2005 May)

Glucagon-like peptides: regulators of cell proliferation, differentiation, and apoptosis.
Drucker DJ
Molecular endocrinology (Baltimore, Md.)17(2)161-71(2003 Feb)

cDNA cloning of proglucagon from the stomach and pancreas of the dog.
Irwin DM
DNA sequence : the journal of DNA sequencing and mapping12(4)253-60(2001 Nov)

The glucagon-like peptides.
Kieffer TJ, Habener JF
Endocrine reviews20(6)876-913(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The higher concentrations of GLP-1 in pituitary-dependent hyperadrenocorticism could play an important role in the impairment of pancreatic beta-cells thus predisposing to diabetes mellitus in dogs.
Title: Involvement of glucagon-like peptide 1 in the glucose homeostasis regulation in obese and pituitary-dependent hyperadrenocorticism affected dogs.

Portal glucagon-like peptide 1 and glucose receptors mediate insulin-independent changes in peripheral glycaemia and determine a strong counter-regulatory response, as reflected by changes in glucagon and cortisol.
Title: Synergistic effect of portal glucose and glucagon-like peptide-1 to lower systemic glucose and stimulate counter-regulatory hormones.

The following GCG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33590	NM_001003044.1 Latest version!	Canis lupus familiaris glucagon (GCG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OCe30954	XM_022414644.1 Latest version!	Canis lupus familiaris glucagon (GCG), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCG ( NM_001003044.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003044.1 Canis lupus familiaris glucagon (GCG), mRNA.

Price & Availability↑

CloneID	OCe33590
Clone ID Related Accession (Same CDS sequence)	NM_001003044.1
Accession Version	NM_001003044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	glucagon precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF308439.1. ##Evidence-Data-START## Transcript exon combination :: AF308439.1, DN356274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN07419779, SAMN07419780 [ECO:0000348] ##Evidence-Data-END##

ATGAAAAGCA TTTACTTTGT GGCTGGATTG TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCTCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTCCTCA GACAGAGCCG 
CTCAATGATC TGGATCAGAT GAATGAAGAC AAGCGTCATT CTCAGGGTAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAGGATT TCGTGCAGTG GTTGATGAAC 
ACCAAGAGGA ACAAGAATAA CATTGCCAAA CGTCATGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG GGGAAGGCGA GATTTCCCAG AAGAAGTTGC CATTGTTGAA 
GAATTCCGTC GCAGACACGC CGACGGTTCC TTCTCTGATG AGATGAACAC AGTTCTTGAT 
ACTCTTGCCA CCCGGGACTT TATAAACTGG CTGCTTCAGA CCAAAATTAC TGACAGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003044.1
CDS	100..642
Translation	MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSAPQTEPLNDLDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNKNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEEFRRRHADGSFSDEMNTVLDTLATRDFINWLLQTKITDRK

Target ORF information:

RefSeq Version	NM_001003044.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucagon (GCG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003044.1

ORF Insert Sequence:

ATGAAAAGCA TTTACTTTGT GGCTGGATTG TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCTCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTCCTCA GACAGAGCCG 
CTCAATGATC TGGATCAGAT GAATGAAGAC AAGCGTCATT CTCAGGGTAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAGGATT TCGTGCAGTG GTTGATGAAC 
ACCAAGAGGA ACAAGAATAA CATTGCCAAA CGTCATGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG GGGAAGGCGA GATTTCCCAG AAGAAGTTGC CATTGTTGAA 
GAATTCCGTC GCAGACACGC CGACGGTTCC TTCTCTGATG AGATGAACAC AGTTCTTGAT 
ACTCTTGCCA CCCGGGACTT TATAAACTGG CTGCTTCAGA CCAAAATTAC TGACAGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCG ( XM_022414644.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270352.1 Canis lupus familiaris glucagon (GCG), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe30954
Clone ID Related Accession (Same CDS sequence)	XM_022414644.1
Accession Version	XM_022414644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	glucagon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006618.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCA TTTACTTTGT GGCTGGATTG TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCTCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTCCTCA GACAGAGCCG 
CTCAATGATC TGGATCAGAT GAATGAAGAC AAGCGTCATT CTCAGGGTAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAGGATT TCGTGCAGTG GTTGATGAAC 
ACCAAGAGGA ACAAGAATAA CATTGCCAAA CGTCATGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG GGGAAGGCGA GATTTCCCAG AAGAAGTTGC CATTGTTGAA 
GAATTGCGTC GCAGACACGC CGACGGTTCT TTCTCTGATG AGATGAACAC AGTTCTTGAT 
ACTCTTGCCA CCCGGGACTT TATAAACTGG CTGCTTCAGA CCAAAATTAC TGACAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270352.1
CDS	249..788
Translation	MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSAPQTEPLNDLDQMNEDKRHSQGTFTSDYSKYLDSRRAQDFVQWLMNTKRNKNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELRRRHADGSFSDEMNTVLDTLATRDFINWLLQTKITDR

Target ORF information:

RefSeq Version	XM_022414644.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucagon (GCG), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022414644.1

ORF Insert Sequence:

ATGAAAAGCA TTTACTTTGT GGCTGGATTG TTTGTAATGC TGGTACAAGG CAGCTGGCAA 
CGTTCTCTTC AAGACACAGA GGAGAAATCC AGATCATTCT CAGCTCCTCA GACAGAGCCG 
CTCAATGATC TGGATCAGAT GAATGAAGAC AAGCGTCATT CTCAGGGTAC ATTCACCAGT 
GACTACAGCA AGTATCTGGA CTCCAGGCGT GCCCAGGATT TCGTGCAGTG GTTGATGAAC 
ACCAAGAGGA ACAAGAATAA CATTGCCAAA CGTCATGATG AATTTGAGAG ACATGCTGAA 
GGGACCTTTA CCAGTGATGT AAGTTCTTAT TTGGAAGGCC AAGCTGCCAA GGAATTCATT 
GCTTGGCTGG TGAAAGGCCG GGGAAGGCGA GATTTCCCAG AAGAAGTTGC CATTGTTGAA 
GAATTGCGTC GCAGACACGC CGACGGTTCT TTCTCTGATG AGATGAACAC AGTTCTTGAT 
ACTCTTGCCA CCCGGGACTT TATAAACTGG CTGCTTCAGA CCAAAATTAC TGACAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Involvement of glucagon-like peptide 1 in the glucose homeostasis regulation in obese and pituitary-dependent hyperadrenocorticism affected dogs.
Research in veterinary science97(2)211-7(2014 Oct)
Miceli DD,Cabrera Blatter MF,Gallelli MF,Pignataro OP,Castillo VA

Synergistic effect of portal glucose and glucagon-like peptide-1 to lower systemic glucose and stimulate counter-regulatory hormones.
Diabetologia48(5)967-75(2005 May)
Ionut V,Hucking K,Liberty IF,Bergman RN

Glucagon-like peptides: regulators of cell proliferation, differentiation, and apoptosis.
Molecular endocrinology (Baltimore, Md.)17(2)161-71(2003 Feb)
Drucker DJ

cDNA cloning of proglucagon from the stomach and pancreas of the dog.
DNA sequence : the journal of DNA sequencing and mapping12(4)253-60(2001 Nov)
Irwin DM

The glucagon-like peptides.
Endocrine reviews20(6)876-913(1999 Dec)
Kieffer TJ,Habener JF