MTOR cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTOR
Entrez Gene ID	478232
Full Name	mechanistic target of rapamycin kinase
Synonyms	FRAP1
General protein information	Preferred Names mechanistic target of rapamycin kinase Names serine/threonine-protein kinase mTOR FK506 binding protein 12-rapamycin associated protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005618003.2	XP_005618060.1	serine/threonine-protein kinase mTOR isoform X1
XM_535407.5	XP_535407.4	serine/threonine-protein kinase mTOR isoform X1
XM_535407.6	XP_535407.4	serine/threonine-protein kinase mTOR isoform X1
XM_005618004.3	XP_005618061.1	serine/threonine-protein kinase mTOR isoform X2
XM_005618004.2	XP_005618061.1	serine/threonine-protein kinase mTOR isoform X2
XM_005618003.3	XP_005618060.1	serine/threonine-protein kinase mTOR isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTOR	NP_004949.1
Canis lupus familiaris (dog)	MTOR	XP_005618060.1
Gallus gallus (chicken)	MTOR	XP_417614.3
Danio rerio (zebrafish)	mtor	NP_001070679.2
Schizosaccharomyces pombe (fission yeast)	tor2	NP_595359.2
Kluyveromyces lactis	KLLA0B13948g	XP_452152.1
Neurospora crassa	NCU05608	XP_960570.1
Pan troglodytes (chimpanzee)	MTOR	XP_003949341.1
Bos taurus (cattle)	MTOR	XP_005217047.1
Drosophila melanogaster (fruit fly)	Tor	NP_001260427.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007873	XP_317619.4
Arabidopsis thaliana (thale cress)	TOR	NP_175425.2
Xenopus tropicalis (tropical clawed frog)	mtor	XP_004916088.1
Rattus norvegicus (Norway rat)	Mtor	NP_063971.1
Oryza sativa (rice)	Os05g0235300	NP_001054988.2
Mus musculus (house mouse)	Mtor	NP_064393.2
Saccharomyces cerevisiae (baker's yeast)	TOR2	NP_012719.2
Eremothecium gossypii	AGOS_AFR420W	NP_985967.2
Magnaporthe oryzae (rice blast fungus)	MGG_15156	XP_003710485.1

Gene Ontology

The following MTOR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTOR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe03882	XM_535407.6 Latest version!	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe03882	XM_005618003.3 Latest version!	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe03883	XM_005618004.3 Latest version!	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
OCe03882	XM_535407.5	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe03883	XM_005618004.2	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe03882	XM_005618003.2	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTOR ( XM_005618003.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618060.1 Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X1, mRNA.

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CloneID	OCe03882
Clone ID Related Accession (Same CDS sequence)	XM_005618003.2 , XM_535407.5 , XM_535407.6 , XM_005618003.3
Accession Version	XM_005618003.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase mTOR isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618060.1
CDS	117..7769
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_005618003.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618003.2

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTOR ( XM_535407.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535407.4 Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X2, mRNA.

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CloneID	OCe03882
Clone ID Related Accession (Same CDS sequence)	XM_005618003.2 , XM_535407.5 , XM_535407.6 , XM_005618003.3
Accession Version	XM_535407.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase mTOR isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_535407.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535407.4
CDS	80..7732
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_535407.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535407.5

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTOR ( XM_535407.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_535407.4 Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X2, mRNA.

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CloneID	OCe03882
Clone ID Related Accession (Same CDS sequence)	XM_005618003.2 , XM_535407.5 , XM_535407.6 , XM_005618003.3
Accession Version	XM_535407.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase mTOR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_535407.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_535407.4
CDS	32..7684
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_535407.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_535407.6

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTOR ( XM_005618003.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618060.1 Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X1, mRNA.

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CloneID	OCe03882
Clone ID Related Accession (Same CDS sequence)	XM_005618003.2 , XM_535407.5 , XM_535407.6 , XM_005618003.3
Accession Version	XM_005618003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase mTOR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618060.1
CDS	124..7776
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_005618003.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618003.3

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGGGATCT CTTTAATGCT 
GCATTTGTGT CCTGCTGGTC TGAACTGAAT GAAGACCAGC AGGATGAACT CATCAGAAGC 
ATCGAGTTGG CCCTCACCTC ACAGGACATT GCCGAAGTCA CGCAGACCCT CTTAAACTTG 
GCTGAATTCA TGGAACACAG TGACAAGGGC CCCCTGCCAT TGAGAGACGA CAATGGCATT 
GTCCTGCTGG GTGAGAGGGC AGCCAAGTGC CGAGCATATG CCAAAGCGCT GCACTACAAA 
GAACTGGAGT TCCAGAAAGG TCCCACCCCC GCCATCCTAG AGTCTCTCAT CAGCATTAAT 
AATAAGCTAC AGCAGCCAGA GGCAGCGGCC GGGGTGTTGG AATATGCCAT GAAACACTTC 
GGAGAGCTGG AGATCCAGGC TACCTGGTAC GAGAAACTGC ATGAGTGGGA GGATGCGCTG 
GTGGCCTATG ATAAGAAGAT GGACACGAAC AAGGATGACC CCGAGCTGAT GCTGGGCCGC 
ATGCGCTGCC TCGAGGCCTT GGGGGAATGG GGGCAGCTCC ACCAGCAGTG CTGTGAAAAG 
TGGACCCTGG TTAATGACGA GACCCAAGCC AAGATGGCCC GCATGGCTGC TGCAGCTGCG 
TGGGGCTTAG GCCAGTGGGA CAGCATGGAG GAGTACACCT GTATGATCCC TCGGGACACC 
CACGATGGGG CATTTTATAG AGCCGTGCTG GCGCTGCATC AGGACCTCTT CTCCTTGGCT 
CAACAGTGCA TCGACAAAGC CAGAGACCTG CTGGATGCGG AGTTAACTGC TATGGCAGGA 
GAGAGCTACA GTCGGGCATA TGGGGCCATG GTTTCTTGCC ACATGCTGTC CGAGCTGGAG 
GAGGTTATCC AGTACAAACT TGTCCCCGAG CGACGGGAGA TCATCCGCCA GATCTGGTGG 
GAGAGACTGC AGGGCTGCCA GCGCATCGTG GAAGACTGGC AGAAAATCCT CATGGTGCGG 
TCGCTGGTGG TCAGCCCTCA CGAGGACATG AGGACCTGGC TCAAGTATGC CAGCCTGTGT 
GGGAAGAGCG GTAGGCTGGC CCTTGCTCAT AAAACCTTAG TGTTGCTCCT GGGAGTCGAC 
CCATCTCGGC AGCTTGACCA TCCTCTGCCA ACAGTGCACC CTCAGGTGAC CTACGCCTAC 
ATGAAAAACA TGTGGAAGAG CACTCGCAAG ATCGATGCCT TCCAGCACAT GCAGCACTTC 
GTGCAGACCA TGCAGCAGCA AGCGCAGCAT GCCATCGCGA CCGAGGACCA GCAGCACAAG 
CAAGAGCTGC ACAAACTCAT GGCCCGGTGT TTCCTGAAAC TCGGGGAGTG GCAGCTGAAC 
CTTCAGGGCA TCAACGAGAG CACAATCCCC AAGGTGCTGC AGTACTACAG CGCTGCCACG 
GAGCATGACC GCAGCTGGTA CAAGGCCTGG CATGCGTGGG CGGTGATGAA CTTTGAAGCT 
GTGCTGCACT ACAAACACCA GAACCAAGCC CGGGACGAGA AGAAGAAGCT GCGTCACGCC 
AGCGGGGCCA ACATCACCAG CACGACCACC GCCGCCACCA CAGCAGCCAC GGCCACGGCC 
ACGGCCACCA GCACCGAGGG CAGCAACAGC GAGAGTGAGG CCGAGAGCAC CGAGAACAGC 
CCCACCCCGT CTCCTCTGCA GAAGAAGGTC ACTGAGGATT TGTCCAAGAC CCTCTTGATG 
TACACGGTCC CCGCCGTCCA GGGCTTCTTC CGTTCCATCT CCTTGTCACG AGGCAACAAC 
CTCCAGGACA CACTGAGAGT CCTCACCTTA TGGTTTGACT ATGGTCACTG GCCGGATGTA 
AACGAAGCCT TGGTGGAGGG GGTGAAAGCG ATCCAGATCG ACACCTGGTT ACAGGTTATA 
CCTCAGCTCA TTGCGAGAAT CGATACGCCT CGGCCCTTGG TGGGCCGCCT CATTCACCAG 
CTTCTCACAG ACATTGGCCG GTACCACCCC CAGGCCCTCA TCTACCCCCT GACCGTGGCC 
TCTAAATCCA CCACGACGGC CCGCCACAAC GCAGCCAACA AGATTCTGAA GAACATGTGC 
GAGCACAGCA ACACCCTGGT CCAGCAGGCC ATGATGGTGA GTGAGGAGCT CATCCGCGTG 
GCCATCCTCT GGCACGAGAT GTGGCACGAA GGCCTGGAAG AAGCGTCACG TTTGTACTTT 
GGGGAGAGGA ACGTGAAAGG CATGTTTGAG GTGCTGGAGC CCCTGCATGC TATGATGGAA 
CGGGGCCCCC AGACTCTGAA GGAAACATCT TTTAATCAGG CATATGGTCG AGACTTAATG 
GAGGCCCAGG AGTGGTGCAG GAAGTACATG AAATCCGGGA ACGTCAAGGA CCTCACCCAG 
GCCTGGGACC TCTATTACCA CGTGTTCAGA CGGATCTCCA AGCAGCTGCC TCAGCTCACC 
TCCCTGGAAC TGCAGTATGT TTCCCCCAAA CTTCTGATGT GCCGGGACCT TGAGTTGGCC 
GTGCCGGGAA CGTACGACCC CAACCAGCCC ATCATTCGCA TTCAGTCCAT AGCACCGTCC 
CTGCAGGTCA TCACCTCCAA GCAGAGGCCC CGGAAGCTGA CGCTCATGGG CAGCAACGGG 
CATGAGTTTG TTTTCCTCCT GAAGGGCCAC GAAGACCTGC GGCAGGATGA GCGGGTGATG 
CAGCTCTTCG GCCTGGTGAA CACCCTTCTG GCCAATGACC CAACGTCTCT TCGCAAGAAC 
CTCAGCATCC AGAGGTACGC TGTCATCCCC TTATCAACCA ACTCGGGCCT CATTGGCTGG 
GTCCCCCACT GCGACACGCT GCACGCCCTC ATCCGGGACT ACAGAGAGAA AAAGAAGATC 
CTTCTCAACA TCGAGCATCG CATCATGTTG CGGATGGCTC CAGACTACGA TCACCTGACG 
CTGATGCAGA AGGTGGAGGT GTTTGAGCAC GCCGTCAACA ACACAGCCGG GGACGACCTG 
GCCAAGCTGC TGTGGCTGAA AAGCCCTAGC TCCGAGGTAT GGTTTGACCG AAGAACCAAC 
TACACCCGCT CTTTAGCGGT CATGTCCATG GTTGGGTATA TTTTGGGCCT GGGAGATAGA 
CACCCATCCA ATCTGATGCT TGACCGGCTG AGTGGGAAGA TCCTGCACAT CGACTTTGGG 
GACTGCTTTG AGGTTGCTAT GACCAGAGAG AAATTCCCAG AGAAGATTCC ATTTAGACTA 
ACGAGAATGC TGACCAATGC TATGGAGGTT ACGGGTCTGG ACGGCAACTA CAGAATCACG 
TGCCACACGG TGATGGAGGT GCTTCGGGAA CACAAGGACA GCGTCATGGC CGTGCTGGAA 
GCCTTTGTCT ACGACCCCTT GCTGAACTGG AGGCTGATGG ACACAAATAC CAAAGGCAAC 
AAGCGGTCCC GAACAAGGAC AGATTCCTAC TCTGCTGGCC AGTCAGTAGA AATTTTGGAT 
GGTGTGGAAC TTGGGGAACC CGCCCACAAG AAAACAGGGA CCACAGTGCC GGAATCTATC 
CATTCTTTCA TTGGAGACGG TTTGGTGAAG CCAGAGGCCC TAAATAAGAA AGCTATCCAG 
ATAATTAACA GGGTTCGAGA TAAGCTCACT GGGCGGGACT TTTCTCACGA TGAAACCCTG 
GATGTCCCGA CACAAGTCGA GCTGCTCATC AAACAGGCCA CATCCCACGA GAACCTGTGT 
CAGTGCTACA TCGGCTGGTG TCCTTTCTGG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTOR ( XM_005618004.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618061.1 Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X3, mRNA.

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CloneID	OCe03883
Clone ID Related Accession (Same CDS sequence)	XM_005618004.3 , XM_005618004.2
Accession Version	XM_005618004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serine/threonine-protein kinase mTOR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGCATTAA TAATAAGCTA 
CAGCAGCCAG AGGCAGCGGC CGGGGTGTTG GAATATGCCA TGAAACACTT CGGAGAGCTG 
GAGATCCAGG CTACCTGGTA CGAGAAACTG CATGAGTGGG AGGATGCGCT GGTGGCCTAT 
GATAAGAAGA TGGACACGAA CAAGGATGAC CCCGAGCTGA TGCTGGGCCG CATGCGCTGC 
CTCGAGGCCT TGGGGGAATG GGGGCAGCTC CACCAGCAGT GCTGTGAAAA GTGGACCCTG 
GTTAATGACG AGACCCAAGC CAAGATGGCC CGCATGGCTG CTGCAGCTGC GTGGGGCTTA 
GGCCAGTGGG ACAGCATGGA GGAGTACACC TGTATGATCC CTCGGGACAC CCACGATGGG 
GCATTTTATA GAGCCGTGCT GGCGCTGCAT CAGGACCTCT TCTCCTTGGC TCAACAGTGC 
ATCGACAAAG CCAGAGACCT GCTGGATGCG GAGTTAACTG CTATGGCAGG AGAGAGCTAC 
AGTCGGGCAT ATGGGGCCAT GGTTTCTTGC CACATGCTGT CCGAGCTGGA GGAGGTTATC 
CAGTACAAAC TTGTCCCCGA GCGACGGGAG ATCATCCGCC AGATCTGGTG GGAGAGACTG 
CAGGGCTGCC AGCGCATCGT GGAAGACTGG CAGAAAATCC TCATGGTGCG GTCGCTGGTG 
GTCAGCCCTC ACGAGGACAT GAGGACCTGG CTCAAGTATG CCAGCCTGTG TGGGAAGAGC 
GGTAGGCTGG CCCTTGCTCA TAAAACCTTA GTGTTGCTCC TGGGAGTCGA CCCATCTCGG 
CAGCTTGACC ATCCTCTGCC AACAGTGCAC CCTCAGGTGA CCTACGCCTA CATGAAAAAC 
ATGTGGAAGA GCACTCGCAA GATCGATGCC TTCCAGCACA TGCAGCACTT CGTGCAGACC 
ATGCAGCAGC AAGCGCAGCA TGCCATCGCG ACCGAGGACC AGCAGCACAA GCAAGAGCTG 
CACAAACTCA TGGCCCGGTG TTTCCTGAAA CTCGGGGAGT GGCAGCTGAA CCTTCAGGGC 
ATCAACGAGA GCACAATCCC CAAGGTGCTG CAGTACTACA GCGCTGCCAC GGAGCATGAC 
CGCAGCTGGT ACAAGGCCTG GCATGCGTGG GCGGTGATGA ACTTTGAAGC TGTGCTGCAC 
TACAAACACC AGAACCAAGC CCGGGACGAG AAGAAGAAGC TGCGTCACGC CAGCGGGGCC 
AACATCACCA GCACGACCAC CGCCGCCACC ACAGCAGCCA CGGCCACGGC CACGGCCACC 
AGCACCGAGG GCAGCAACAG CGAGAGTGAG GCCGAGAGCA CCGAGAACAG CCCCACCCCG 
TCTCCTCTGC AGAAGAAGGT CACTGAGGAT TTGTCCAAGA CCCTCTTGAT GTACACGGTC 
CCCGCCGTCC AGGGCTTCTT CCGTTCCATC TCCTTGTCAC GAGGCAACAA CCTCCAGGAC 
ACACTGAGAG TCCTCACCTT ATGGTTTGAC TATGGTCACT GGCCGGATGT AAACGAAGCC 
TTGGTGGAGG GGGTGAAAGC GATCCAGATC GACACCTGGT TACAGGTTAT ACCTCAGCTC 
ATTGCGAGAA TCGATACGCC TCGGCCCTTG GTGGGCCGCC TCATTCACCA GCTTCTCACA 
GACATTGGCC GGTACCACCC CCAGGCCCTC ATCTACCCCC TGACCGTGGC CTCTAAATCC 
ACCACGACGG CCCGCCACAA CGCAGCCAAC AAGATTCTGA AGAACATGTG CGAGCACAGC 
AACACCCTGG TCCAGCAGGC CATGATGGTG AGTGAGGAGC TCATCCGCGT GGCCATCCTC 
TGGCACGAGA TGTGGCACGA AGGCCTGGAA GAAGCGTCAC GTTTGTACTT TGGGGAGAGG 
AACGTGAAAG GCATGTTTGA GGTGCTGGAG CCCCTGCATG CTATGATGGA ACGGGGCCCC 
CAGACTCTGA AGGAAACATC TTTTAATCAG GCATATGGTC GAGACTTAAT GGAGGCCCAG 
GAGTGGTGCA GGAAGTACAT GAAATCCGGG AACGTCAAGG ACCTCACCCA GGCCTGGGAC 
CTCTATTACC ACGTGTTCAG ACGGATCTCC AAGCAGCTGC CTCAGCTCAC CTCCCTGGAA 
CTGCAGTATG TTTCCCCCAA ACTTCTGATG TGCCGGGACC TTGAGTTGGC CGTGCCGGGA 
ACGTACGACC CCAACCAGCC CATCATTCGC ATTCAGTCCA TAGCACCGTC CCTGCAGGTC 
ATCACCTCCA AGCAGAGGCC CCGGAAGCTG ACGCTCATGG GCAGCAACGG GCATGAGTTT 
GTTTTCCTCC TGAAGGGCCA CGAAGACCTG CGGCAGGATG AGCGGGTGAT GCAGCTCTTC 
GGCCTGGTGA ACACCCTTCT GGCCAATGAC CCAACGTCTC TTCGCAAGAA CCTCAGCATC 
CAGAGGTACG CTGTCATCCC CTTATCAACC AACTCGGGCC TCATTGGCTG GGTCCCCCAC 
TGCGACACGC TGCACGCCCT CATCCGGGAC TACAGAGAGA AAAAGAAGAT CCTTCTCAAC 
ATCGAGCATC GCATCATGTT GCGGATGGCT CCAGACTACG ATCACCTGAC GCTGATGCAG 
AAGGTGGAGG TGTTTGAGCA CGCCGTCAAC AACACAGCCG GGGACGACCT GGCCAAGCTG 
CTGTGGCTGA AAAGCCCTAG CTCCGAGGTA TGGTTTGACC GAAGAACCAA CTACACCCGC 
TCTTTAGCGG TCATGTCCAT GGTTGGGTAT ATTTTGGGCC TGGGAGATAG ACACCCATCC 
AATCTGATGC TTGACCGGCT GAGTGGGAAG ATCCTGCACA TCGACTTTGG GGACTGCTTT 
GAGGTTGCTA TGACCAGAGA GAAATTCCCA GAGAAGATTC CATTTAGACT AACGAGAATG 
CTGACCAATG CTATGGAGGT TACGGGTCTG GACGGCAACT ACAGAATCAC GTGCCACACG 
GTGATGGAGG TGCTTCGGGA ACACAAGGAC AGCGTCATGG CCGTGCTGGA AGCCTTTGTC 
TACGACCCCT TGCTGAACTG GAGGCTGATG GACACAAATA CCAAAGGCAA CAAGCGGTCC 
CGAACAAGGA CAGATTCCTA CTCTGCTGGC CAGTCAGTAG AAATTTTGGA TGGTGTGGAA 
CTTGGGGAAC CCGCCCACAA GAAAACAGGG ACCACAGTGC CGGAATCTAT CCATTCTTTC 
ATTGGAGACG GTTTGGTGAA GCCAGAGGCC CTAAATAAGA AAGCTATCCA GATAATTAAC 
AGGGTTCGAG ATAAGCTCAC TGGGCGGGAC TTTTCTCACG ATGAAACCCT GGATGTCCCG 
ACACAAGTCG AGCTGCTCAT CAAACAGGCC ACATCCCACG AGAACCTGTG TCAGTGCTAC 
ATCGGCTGGT GTCCTTTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618061.1
CDS	124..7467
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMASINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_005618004.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin kinase (MTOR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618004.3

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGCATTAA TAATAAGCTA 
CAGCAGCCAG AGGCAGCGGC CGGGGTGTTG GAATATGCCA TGAAACACTT CGGAGAGCTG 
GAGATCCAGG CTACCTGGTA CGAGAAACTG CATGAGTGGG AGGATGCGCT GGTGGCCTAT 
GATAAGAAGA TGGACACGAA CAAGGATGAC CCCGAGCTGA TGCTGGGCCG CATGCGCTGC 
CTCGAGGCCT TGGGGGAATG GGGGCAGCTC CACCAGCAGT GCTGTGAAAA GTGGACCCTG 
GTTAATGACG AGACCCAAGC CAAGATGGCC CGCATGGCTG CTGCAGCTGC GTGGGGCTTA 
GGCCAGTGGG ACAGCATGGA GGAGTACACC TGTATGATCC CTCGGGACAC CCACGATGGG 
GCATTTTATA GAGCCGTGCT GGCGCTGCAT CAGGACCTCT TCTCCTTGGC TCAACAGTGC 
ATCGACAAAG CCAGAGACCT GCTGGATGCG GAGTTAACTG CTATGGCAGG AGAGAGCTAC 
AGTCGGGCAT ATGGGGCCAT GGTTTCTTGC CACATGCTGT CCGAGCTGGA GGAGGTTATC 
CAGTACAAAC TTGTCCCCGA GCGACGGGAG ATCATCCGCC AGATCTGGTG GGAGAGACTG 
CAGGGCTGCC AGCGCATCGT GGAAGACTGG CAGAAAATCC TCATGGTGCG GTCGCTGGTG 
GTCAGCCCTC ACGAGGACAT GAGGACCTGG CTCAAGTATG CCAGCCTGTG TGGGAAGAGC 
GGTAGGCTGG CCCTTGCTCA TAAAACCTTA GTGTTGCTCC TGGGAGTCGA CCCATCTCGG 
CAGCTTGACC ATCCTCTGCC AACAGTGCAC CCTCAGGTGA CCTACGCCTA CATGAAAAAC 
ATGTGGAAGA GCACTCGCAA GATCGATGCC TTCCAGCACA TGCAGCACTT CGTGCAGACC 
ATGCAGCAGC AAGCGCAGCA TGCCATCGCG ACCGAGGACC AGCAGCACAA GCAAGAGCTG 
CACAAACTCA TGGCCCGGTG TTTCCTGAAA CTCGGGGAGT GGCAGCTGAA CCTTCAGGGC 
ATCAACGAGA GCACAATCCC CAAGGTGCTG CAGTACTACA GCGCTGCCAC GGAGCATGAC 
CGCAGCTGGT ACAAGGCCTG GCATGCGTGG GCGGTGATGA ACTTTGAAGC TGTGCTGCAC 
TACAAACACC AGAACCAAGC CCGGGACGAG AAGAAGAAGC TGCGTCACGC CAGCGGGGCC 
AACATCACCA GCACGACCAC CGCCGCCACC ACAGCAGCCA CGGCCACGGC CACGGCCACC 
AGCACCGAGG GCAGCAACAG CGAGAGTGAG GCCGAGAGCA CCGAGAACAG CCCCACCCCG 
TCTCCTCTGC AGAAGAAGGT CACTGAGGAT TTGTCCAAGA CCCTCTTGAT GTACACGGTC 
CCCGCCGTCC AGGGCTTCTT CCGTTCCATC TCCTTGTCAC GAGGCAACAA CCTCCAGGAC 
ACACTGAGAG TCCTCACCTT ATGGTTTGAC TATGGTCACT GGCCGGATGT AAACGAAGCC 
TTGGTGGAGG GGGTGAAAGC GATCCAGATC GACACCTGGT TACAGGTTAT ACCTCAGCTC 
ATTGCGAGAA TCGATACGCC TCGGCCCTTG GTGGGCCGCC TCATTCACCA GCTTCTCACA 
GACATTGGCC GGTACCACCC CCAGGCCCTC ATCTACCCCC TGACCGTGGC CTCTAAATCC 
ACCACGACGG CCCGCCACAA CGCAGCCAAC AAGATTCTGA AGAACATGTG CGAGCACAGC 
AACACCCTGG TCCAGCAGGC CATGATGGTG AGTGAGGAGC TCATCCGCGT GGCCATCCTC 
TGGCACGAGA TGTGGCACGA AGGCCTGGAA GAAGCGTCAC GTTTGTACTT TGGGGAGAGG 
AACGTGAAAG GCATGTTTGA GGTGCTGGAG CCCCTGCATG CTATGATGGA ACGGGGCCCC 
CAGACTCTGA AGGAAACATC TTTTAATCAG GCATATGGTC GAGACTTAAT GGAGGCCCAG 
GAGTGGTGCA GGAAGTACAT GAAATCCGGG AACGTCAAGG ACCTCACCCA GGCCTGGGAC 
CTCTATTACC ACGTGTTCAG ACGGATCTCC AAGCAGCTGC CTCAGCTCAC CTCCCTGGAA 
CTGCAGTATG TTTCCCCCAA ACTTCTGATG TGCCGGGACC TTGAGTTGGC CGTGCCGGGA 
ACGTACGACC CCAACCAGCC CATCATTCGC ATTCAGTCCA TAGCACCGTC CCTGCAGGTC 
ATCACCTCCA AGCAGAGGCC CCGGAAGCTG ACGCTCATGG GCAGCAACGG GCATGAGTTT 
GTTTTCCTCC TGAAGGGCCA CGAAGACCTG CGGCAGGATG AGCGGGTGAT GCAGCTCTTC 
GGCCTGGTGA ACACCCTTCT GGCCAATGAC CCAACGTCTC TTCGCAAGAA CCTCAGCATC 
CAGAGGTACG CTGTCATCCC CTTATCAACC AACTCGGGCC TCATTGGCTG GGTCCCCCAC 
TGCGACACGC TGCACGCCCT CATCCGGGAC TACAGAGAGA AAAAGAAGAT CCTTCTCAAC 
ATCGAGCATC GCATCATGTT GCGGATGGCT CCAGACTACG ATCACCTGAC GCTGATGCAG 
AAGGTGGAGG TGTTTGAGCA CGCCGTCAAC AACACAGCCG GGGACGACCT GGCCAAGCTG 
CTGTGGCTGA AAAGCCCTAG CTCCGAGGTA TGGTTTGACC GAAGAACCAA CTACACCCGC 
TCTTTAGCGG TCATGTCCAT GGTTGGGTAT ATTTTGGGCC TGGGAGATAG ACACCCATCC 
AATCTGATGC TTGACCGGCT GAGTGGGAAG ATCCTGCACA TCGACTTTGG GGACTGCTTT 
GAGGTTGCTA TGACCAGAGA GAAATTCCCA GAGAAGATTC CATTTAGACT AACGAGAATG 
CTGACCAATG CTATGGAGGT TACGGGTCTG GACGGCAACT ACAGAATCAC GTGCCACACG 
GTGATGGAGG TGCTTCGGGA ACACAAGGAC AGCGTCATGG CCGTGCTGGA AGCCTTTGTC 
TACGACCCCT TGCTGAACTG GAGGCTGATG GACACAAATA CCAAAGGCAA CAAGCGGTCC 
CGAACAAGGA CAGATTCCTA CTCTGCTGGC CAGTCAGTAG AAATTTTGGA TGGTGTGGAA 
CTTGGGGAAC CCGCCCACAA GAAAACAGGG ACCACAGTGC CGGAATCTAT CCATTCTTTC 
ATTGGAGACG GTTTGGTGAA GCCAGAGGCC CTAAATAAGA AAGCTATCCA GATAATTAAC 
AGGGTTCGAG ATAAGCTCAC TGGGCGGGAC TTTTCTCACG ATGAAACCCT GGATGTCCCG 
ACACAAGTCG AGCTGCTCAT CAAACAGGCC ACATCCCACG AGAACCTGTG TCAGTGCTAC 
ATCGGCTGGT GTCCTTTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTOR ( XM_005618004.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618061.1 Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X3, mRNA.

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CloneID	OCe03883
Clone ID Related Accession (Same CDS sequence)	XM_005618004.3 , XM_005618004.2
Accession Version	XM_005618004.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serine/threonine-protein kinase mTOR isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726051.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGCATTAA TAATAAGCTA 
CAGCAGCCAG AGGCAGCGGC CGGGGTGTTG GAATATGCCA TGAAACACTT CGGAGAGCTG 
GAGATCCAGG CTACCTGGTA CGAGAAACTG CATGAGTGGG AGGATGCGCT GGTGGCCTAT 
GATAAGAAGA TGGACACGAA CAAGGATGAC CCCGAGCTGA TGCTGGGCCG CATGCGCTGC 
CTCGAGGCCT TGGGGGAATG GGGGCAGCTC CACCAGCAGT GCTGTGAAAA GTGGACCCTG 
GTTAATGACG AGACCCAAGC CAAGATGGCC CGCATGGCTG CTGCAGCTGC GTGGGGCTTA 
GGCCAGTGGG ACAGCATGGA GGAGTACACC TGTATGATCC CTCGGGACAC CCACGATGGG 
GCATTTTATA GAGCCGTGCT GGCGCTGCAT CAGGACCTCT TCTCCTTGGC TCAACAGTGC 
ATCGACAAAG CCAGAGACCT GCTGGATGCG GAGTTAACTG CTATGGCAGG AGAGAGCTAC 
AGTCGGGCAT ATGGGGCCAT GGTTTCTTGC CACATGCTGT CCGAGCTGGA GGAGGTTATC 
CAGTACAAAC TTGTCCCCGA GCGACGGGAG ATCATCCGCC AGATCTGGTG GGAGAGACTG 
CAGGGCTGCC AGCGCATCGT GGAAGACTGG CAGAAAATCC TCATGGTGCG GTCGCTGGTG 
GTCAGCCCTC ACGAGGACAT GAGGACCTGG CTCAAGTATG CCAGCCTGTG TGGGAAGAGC 
GGTAGGCTGG CCCTTGCTCA TAAAACCTTA GTGTTGCTCC TGGGAGTCGA CCCATCTCGG 
CAGCTTGACC ATCCTCTGCC AACAGTGCAC CCTCAGGTGA CCTACGCCTA CATGAAAAAC 
ATGTGGAAGA GCACTCGCAA GATCGATGCC TTCCAGCACA TGCAGCACTT CGTGCAGACC 
ATGCAGCAGC AAGCGCAGCA TGCCATCGCG ACCGAGGACC AGCAGCACAA GCAAGAGCTG 
CACAAACTCA TGGCCCGGTG TTTCCTGAAA CTCGGGGAGT GGCAGCTGAA CCTTCAGGGC 
ATCAACGAGA GCACAATCCC CAAGGTGCTG CAGTACTACA GCGCTGCCAC GGAGCATGAC 
CGCAGCTGGT ACAAGGCCTG GCATGCGTGG GCGGTGATGA ACTTTGAAGC TGTGCTGCAC 
TACAAACACC AGAACCAAGC CCGGGACGAG AAGAAGAAGC TGCGTCACGC CAGCGGGGCC 
AACATCACCA GCACGACCAC CGCCGCCACC ACAGCAGCCA CGGCCACGGC CACGGCCACC 
AGCACCGAGG GCAGCAACAG CGAGAGTGAG GCCGAGAGCA CCGAGAACAG CCCCACCCCG 
TCTCCTCTGC AGAAGAAGGT CACTGAGGAT TTGTCCAAGA CCCTCTTGAT GTACACGGTC 
CCCGCCGTCC AGGGCTTCTT CCGTTCCATC TCCTTGTCAC GAGGCAACAA CCTCCAGGAC 
ACACTGAGAG TCCTCACCTT ATGGTTTGAC TATGGTCACT GGCCGGATGT AAACGAAGCC 
TTGGTGGAGG GGGTGAAAGC GATCCAGATC GACACCTGGT TACAGGTTAT ACCTCAGCTC 
ATTGCGAGAA TCGATACGCC TCGGCCCTTG GTGGGCCGCC TCATTCACCA GCTTCTCACA 
GACATTGGCC GGTACCACCC CCAGGCCCTC ATCTACCCCC TGACCGTGGC CTCTAAATCC 
ACCACGACGG CCCGCCACAA CGCAGCCAAC AAGATTCTGA AGAACATGTG CGAGCACAGC 
AACACCCTGG TCCAGCAGGC CATGATGGTG AGTGAGGAGC TCATCCGCGT GGCCATCCTC 
TGGCACGAGA TGTGGCACGA AGGCCTGGAA GAAGCGTCAC GTTTGTACTT TGGGGAGAGG 
AACGTGAAAG GCATGTTTGA GGTGCTGGAG CCCCTGCATG CTATGATGGA ACGGGGCCCC 
CAGACTCTGA AGGAAACATC TTTTAATCAG GCATATGGTC GAGACTTAAT GGAGGCCCAG 
GAGTGGTGCA GGAAGTACAT GAAATCCGGG AACGTCAAGG ACCTCACCCA GGCCTGGGAC 
CTCTATTACC ACGTGTTCAG ACGGATCTCC AAGCAGCTGC CTCAGCTCAC CTCCCTGGAA 
CTGCAGTATG TTTCCCCCAA ACTTCTGATG TGCCGGGACC TTGAGTTGGC CGTGCCGGGA 
ACGTACGACC CCAACCAGCC CATCATTCGC ATTCAGTCCA TAGCACCGTC CCTGCAGGTC 
ATCACCTCCA AGCAGAGGCC CCGGAAGCTG ACGCTCATGG GCAGCAACGG GCATGAGTTT 
GTTTTCCTCC TGAAGGGCCA CGAAGACCTG CGGCAGGATG AGCGGGTGAT GCAGCTCTTC 
GGCCTGGTGA ACACCCTTCT GGCCAATGAC CCAACGTCTC TTCGCAAGAA CCTCAGCATC 
CAGAGGTACG CTGTCATCCC CTTATCAACC AACTCGGGCC TCATTGGCTG GGTCCCCCAC 
TGCGACACGC TGCACGCCCT CATCCGGGAC TACAGAGAGA AAAAGAAGAT CCTTCTCAAC 
ATCGAGCATC GCATCATGTT GCGGATGGCT CCAGACTACG ATCACCTGAC GCTGATGCAG 
AAGGTGGAGG TGTTTGAGCA CGCCGTCAAC AACACAGCCG GGGACGACCT GGCCAAGCTG 
CTGTGGCTGA AAAGCCCTAG CTCCGAGGTA TGGTTTGACC GAAGAACCAA CTACACCCGC 
TCTTTAGCGG TCATGTCCAT GGTTGGGTAT ATTTTGGGCC TGGGAGATAG ACACCCATCC 
AATCTGATGC TTGACCGGCT GAGTGGGAAG ATCCTGCACA TCGACTTTGG GGACTGCTTT 
GAGGTTGCTA TGACCAGAGA GAAATTCCCA GAGAAGATTC CATTTAGACT AACGAGAATG 
CTGACCAATG CTATGGAGGT TACGGGTCTG GACGGCAACT ACAGAATCAC GTGCCACACG 
GTGATGGAGG TGCTTCGGGA ACACAAGGAC AGCGTCATGG CCGTGCTGGA AGCCTTTGTC 
TACGACCCCT TGCTGAACTG GAGGCTGATG GACACAAATA CCAAAGGCAA CAAGCGGTCC 
CGAACAAGGA CAGATTCCTA CTCTGCTGGC CAGTCAGTAG AAATTTTGGA TGGTGTGGAA 
CTTGGGGAAC CCGCCCACAA GAAAACAGGG ACCACAGTGC CGGAATCTAT CCATTCTTTC 
ATTGGAGACG GTTTGGTGAA GCCAGAGGCC CTAAATAAGA AAGCTATCCA GATAATTAAC 
AGGGTTCGAG ATAAGCTCAC TGGGCGGGAC TTTTCTCACG ATGAAACCCT GGATGTCCCG 
ACACAAGTCG AGCTGCTCAT CAAACAGGCC ACATCCCACG AGAACCTGTG TCAGTGCTAC 
ATCGGCTGGT GTCCTTTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618061.1
CDS	117..7460
Translation	MLGTGPAAATAATTTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAVSVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPPQSNALVGLLGYSSHQGLMGFGASPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTALPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSVHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEVPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRPTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMASINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSTRKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITSTTTAATTAATATATATSTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDETLDVPTQVELLIKQATSHENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	XM_005618004.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mechanistic target of rapamycin (serine/threonine kinase) (MTOR), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618004.2

ORF Insert Sequence:

ATGCTTGGAA CCGGACCTGC TGCCGCCACC GCAGCCACCA CCACGTCGAG TAACGTGAGC 
GTCCTACAGC AGTTCGCCAG CGGCCTGAAG AGCCGGAATG AGGAGACCAG GGCCAAGGCT 
GCCAAGGAGC TCCAACACTA TGTCACCATG GAGCTCCGAG AGATGAGTCA AGAGGAGTCT 
ACTCGCTTCT ATGACCAGCT GAACCATCAC ATTTTTGAAC TGGTTTCCAG CTCAGATGCC 
AATGAAAGGA AAGGTGGCAT CTTGGCCATA GCCAGCCTCA TAGGAGTGGA AGGTGGGAAT 
GCCACTCGGA TTGGCAGATT CGCTAACTAT CTTCGGAACC TCCTCCCCTC TAATGACCCA 
GTTGTAATGG AGATGGCATC CAAGGCCATT GGCCGCCTTG CCATGGCAGG GGACACTTTT 
ACGGCTGAGT ATGTGGAGTT TGAGGTGAAG CGAGCCTTGG AGTGGCTGGG CGCCGACCGC 
AACGAGGGCC GGAGACACGC AGCTGTTCTA GTTCTCCGTG AGCTGGCTGT CAGCGTCCCC 
ACCTTCTTCT TCCAGCAAGT GCAACCCTTC TTTGACAACA TTTTTGTGGC TGTATGGGAC 
CCCAAACAGG CCATCCGTGA GGGAGCTGTG GCTGCCCTTC GTGCCTGTTT GATCCTCACC 
ACCCAGCGTG AGCCGAAGGA GATGCAGAAG CCTCAGTGGT ATCGGCACAC GTTTGAAGAA 
GCAGAGAAAG GGTTTGATGA GACATTGGCC AAGGAGAAGG GCATGAATCG GGATGATCGC 
ATCCATGGAG CCTTGCTGAT CCTCAACGAG TTGGTCCGAA TCAGCAGCAT GGAGGGAGAG 
CGCCTGAGAG AAGAAATGGA GGAGATCACG CAGCAGCAGC TGGTGCACGA CAAGTACTGC 
AAGGACCTCA TGGGCTTCGG GACGAAACCT CGCCACATCA CGCCCTTCAC CAGTTTCCAG 
GCTGTCCAGC CCCCGCAGTC CAACGCCTTG GTGGGACTGC TGGGGTACAG CTCTCACCAA 
GGCCTCATGG GGTTCGGGGC TTCCCCCAGC CCAGCCAAGT CCACCCTGGT GGAGAGTCGG 
TGTTGCCGAG ACCTGATGGA GGAGAAGTTC GATCAGGTGT GCCAGTGGGT GCTGAAGTGC 
AGGAACAGCA AGAACTCGCT GATCCAAATG ACAATCCTTA ATTTGTTGCC CCGCCTGGCC 
GCGTTCCGAC CTTCTGCCTT CACAGATACC CAGTACCTCC AAGATACCAT GAACCATGTG 
TTAAGCTGCG TCAAGAAGGA GAAGGAACGT ACAGCAGCCT TCCAAGCCCT GGGGCTGCTT 
TCCGTGGCTG TGAGATCTGA GTTTAAGGTC TATTTACCTC GTGTGCTGGA CATCATCCGT 
GCAGCCCTAC CCCCTAAGGA CTTTGCCCAT AAGAGACAGA AGGCCATGCA GGTGGATGCC 
ACAGTGTTCA CCTGCATCAG CATGCTGGCC CGGGCAATGG GGCCGGGCAT CCAACAAGAT 
ATCAAGGAGC TGCTGGAGCC CATGCTGGCA GTGGGACTGA GCCCTGCCCT CACTGCTGTG 
CTCTACGACC TGAGCCGTCA GATTCCGCAG CTGAAGAAGG ACATCCAGGA TGGGCTCCTG 
AAGATGCTGT CCCTGGTCCT GATGCACAAA CCCCTCCGGC ACCCGGGCAT GCCCAAGGGC 
CTGGCCCACC AGCTAGCTTC CCCCGGCCTC ACAGCCCTCC CCGAGGCCAG CGATGTGGGC 
AGCATTACTC TTGCCCTGCG AACTCTTGGC AGCTTTGAGT TTGAAGGCCA CTCTCTGACC 
CAGTTTGTCC GCCACTGTGC GGATCATTTC CTAAACAGCG AGCACAAGGA GATCCGCATG 
GAAGCTGCCC GCACCTGCTC CCGCCTGCTC ACGCCCTCCG TCCACCTCAT CAGTGGCCAC 
GCTCATGTGG TCAGCCAGAC TGCAGTGCAA GTGGTGGCAG ACGTGCTCAG CAAACTGCTT 
GTGGTTGGGA TAACAGATCC TGACCCTGAC ATCCGCTACT GTGTCTTGGC GTCCCTGGAT 
GAGCGCTTTG ATGCACACCT GGCCCAGGCA GAGAATTTGC AGGCCCTGTT TGTGGCCCTG 
AACGACCAGG TGTTTGAGAT CCGGGAGCTG GCCATCTGCA CCGTGGGCCG GCTCAGTAGC 
ATGAACCCGG CCTTCGTCAT GCCTTTCCTG CGCAAGATGC TCATCCAGAT TCTGACAGAG 
CTGGAGCACA GCGGTATTGG AAGAATCAAA GAGCAGAGTG CCCGCATGCT GGGGCACCTG 
GTCTCCAATG CCCCCCGGCT CATCCGCCCC TACATGGAGC CCATCCTGAA GGCTTTAATT 
TTGAAACTGA AAGATCCAGA CCCTGATCCA AACCCAGGTG TGATCAACAA TGTCTTGGCT 
ACAATTGGAG AATTGGCTCA GGTTAGTGGC CTAGAGATGA GGAAGTGGGT GGATGAACTC 
TTTATTATCA TCATGGACAT GCTCCAGGAC TCCTCTCTGT TGGCCAAAAG GCAGGTGGCT 
CTGTGGACCC TGGGACAGTT GGTGGCCAGC ACTGGCTACG TGGTGGAGCC CTACCGGAAG 
TACCCCACTT TGCTTGAGGT CCTGCTGAAT TTTCTGAAGA CTGAGCAGAA CCAGGGTACA 
CGGAGAGAGG CTATCCGTGT GTTAGGGCTT TTGGGGGCCC TGGATCCCTA CAAGCACAAA 
GTGAACATCG GCATGATTGA CCAGTCCCGC GACGCTTCTG CTGTCAGCCT GTCTGAATCC 
AAGTCAAGTC AGGATTCCTC CGACTACAGC ACCAGTGAGA TGCTGGTCAA CATGGGCAAC 
CTGCCCCTGG ACGAGTTCTA CCCCGCCGTG TCCATGGTGG CGCTGATGCG GATCTTCCGA 
GACCAGTCCC TCTCGCACCA TCACACCATG GTTGTCCAGG CCATCACCTT CATCTTCAAG 
TCCCTGGGCC TCAAGTGTGT GCAGTTTCTG CCGCAGGTCA TGCCCACATT CCTCAATGTC 
ATTCGGGTCT GCGATGGGGC CATCCGGGAA TTCCTGTTCC AGCAGCTGGG AATGTTGGTG 
TCCTTTGTGA AGAGCCACAT CAGACCTTAC ATGGATGAAA TAGTCACCCT CATGAGAGAA 
TTCTGGGTCA TGAACACCTC GATCCAGAGC ACAATCATTC TTCTCATTGA GCAAATTGTG 
GTAGCTCTTG GGGGTGAATT TAAGCTCTAC CTGCCCCAGC TGATCCCACA CATGCTCCGC 
GTCTTCATGC ATGATAACAG CCCAGGCCGC ATCGTCTCCA TCAAGCTGTT GGCTGCCATC 
CAGCTGTTTG GTGCCAACCT GGATGACTAT CTGCATTTGT TGTTGCCTCC TATCGTGAAG 
CTGTTTGACG CCCCGGAAGT TCCCCTGCCG TCTCGGAAGG CAGCGCTAGA GACCGTAGAC 
CGCCTGACAG AGTCCCTGGA CTTCACTGAC TATGCCTCCC GTATCATTCA TCCGATTGTT 
CGGACGCTAG ACCAGAGCCC GGAGCTGCGT CCCACAGCCA TGGACACATT GTCTTCACTG 
GTCTTCCAAC TGGGGAAGAA GTACCAAATT TTCATTCCAA TGGTGAATAA AGTCCTGGTG 
CGACACCGAA TCAACCATCA GCGCTACGAC GTCCTCATCT GCAGAATCGT CAAGGGATAC 
ACCCTTGCTG ACGAGGAAGA GGACCCTTTG ATTTATCAGC ATCGAATGCT GAGGAGCGGC 
CAAGGGGATG CGTTGGCTAG TGGGCCAGTA GAAACGGGAC CCATGAAGAA ACTGCACGTC 
AGCACCATCA ACCTCCAAAA GGCCTGGGGA GCTGCCAGAA GGGTCTCCAA AGACGACTGG 
CTGGAATGGC TGAGGCGGCT GAGTCTGGAG CTCCTGAAGG ACTCCTCGTC GCCTTCCCTG 
CGCTCGTGCT GGGCCCTGGC TCAGGCCTAC AACCCGATGG CCAGCATTAA TAATAAGCTA 
CAGCAGCCAG AGGCAGCGGC CGGGGTGTTG GAATATGCCA TGAAACACTT CGGAGAGCTG 
GAGATCCAGG CTACCTGGTA CGAGAAACTG CATGAGTGGG AGGATGCGCT GGTGGCCTAT 
GATAAGAAGA TGGACACGAA CAAGGATGAC CCCGAGCTGA TGCTGGGCCG CATGCGCTGC 
CTCGAGGCCT TGGGGGAATG GGGGCAGCTC CACCAGCAGT GCTGTGAAAA GTGGACCCTG 
GTTAATGACG AGACCCAAGC CAAGATGGCC CGCATGGCTG CTGCAGCTGC GTGGGGCTTA 
GGCCAGTGGG ACAGCATGGA GGAGTACACC TGTATGATCC CTCGGGACAC CCACGATGGG 
GCATTTTATA GAGCCGTGCT GGCGCTGCAT CAGGACCTCT TCTCCTTGGC TCAACAGTGC 
ATCGACAAAG CCAGAGACCT GCTGGATGCG GAGTTAACTG CTATGGCAGG AGAGAGCTAC 
AGTCGGGCAT ATGGGGCCAT GGTTTCTTGC CACATGCTGT CCGAGCTGGA GGAGGTTATC 
CAGTACAAAC TTGTCCCCGA GCGACGGGAG ATCATCCGCC AGATCTGGTG GGAGAGACTG 
CAGGGCTGCC AGCGCATCGT GGAAGACTGG CAGAAAATCC TCATGGTGCG GTCGCTGGTG 
GTCAGCCCTC ACGAGGACAT GAGGACCTGG CTCAAGTATG CCAGCCTGTG TGGGAAGAGC 
GGTAGGCTGG CCCTTGCTCA TAAAACCTTA GTGTTGCTCC TGGGAGTCGA CCCATCTCGG 
CAGCTTGACC ATCCTCTGCC AACAGTGCAC CCTCAGGTGA CCTACGCCTA CATGAAAAAC 
ATGTGGAAGA GCACTCGCAA GATCGATGCC TTCCAGCACA TGCAGCACTT CGTGCAGACC 
ATGCAGCAGC AAGCGCAGCA TGCCATCGCG ACCGAGGACC AGCAGCACAA GCAAGAGCTG 
CACAAACTCA TGGCCCGGTG TTTCCTGAAA CTCGGGGAGT GGCAGCTGAA CCTTCAGGGC 
ATCAACGAGA GCACAATCCC CAAGGTGCTG CAGTACTACA GCGCTGCCAC GGAGCATGAC 
CGCAGCTGGT ACAAGGCCTG GCATGCGTGG GCGGTGATGA ACTTTGAAGC TGTGCTGCAC 
TACAAACACC AGAACCAAGC CCGGGACGAG AAGAAGAAGC TGCGTCACGC CAGCGGGGCC 
AACATCACCA GCACGACCAC CGCCGCCACC ACAGCAGCCA CGGCCACGGC CACGGCCACC 
AGCACCGAGG GCAGCAACAG CGAGAGTGAG GCCGAGAGCA CCGAGAACAG CCCCACCCCG 
TCTCCTCTGC AGAAGAAGGT CACTGAGGAT TTGTCCAAGA CCCTCTTGAT GTACACGGTC 
CCCGCCGTCC AGGGCTTCTT CCGTTCCATC TCCTTGTCAC GAGGCAACAA CCTCCAGGAC 
ACACTGAGAG TCCTCACCTT ATGGTTTGAC TATGGTCACT GGCCGGATGT AAACGAAGCC 
TTGGTGGAGG GGGTGAAAGC GATCCAGATC GACACCTGGT TACAGGTTAT ACCTCAGCTC 
ATTGCGAGAA TCGATACGCC TCGGCCCTTG GTGGGCCGCC TCATTCACCA GCTTCTCACA 
GACATTGGCC GGTACCACCC CCAGGCCCTC ATCTACCCCC TGACCGTGGC CTCTAAATCC 
ACCACGACGG CCCGCCACAA CGCAGCCAAC AAGATTCTGA AGAACATGTG CGAGCACAGC 
AACACCCTGG TCCAGCAGGC CATGATGGTG AGTGAGGAGC TCATCCGCGT GGCCATCCTC 
TGGCACGAGA TGTGGCACGA AGGCCTGGAA GAAGCGTCAC GTTTGTACTT TGGGGAGAGG 
AACGTGAAAG GCATGTTTGA GGTGCTGGAG CCCCTGCATG CTATGATGGA ACGGGGCCCC 
CAGACTCTGA AGGAAACATC TTTTAATCAG GCATATGGTC GAGACTTAAT GGAGGCCCAG 
GAGTGGTGCA GGAAGTACAT GAAATCCGGG AACGTCAAGG ACCTCACCCA GGCCTGGGAC 
CTCTATTACC ACGTGTTCAG ACGGATCTCC AAGCAGCTGC CTCAGCTCAC CTCCCTGGAA 
CTGCAGTATG TTTCCCCCAA ACTTCTGATG TGCCGGGACC TTGAGTTGGC CGTGCCGGGA 
ACGTACGACC CCAACCAGCC CATCATTCGC ATTCAGTCCA TAGCACCGTC CCTGCAGGTC 
ATCACCTCCA AGCAGAGGCC CCGGAAGCTG ACGCTCATGG GCAGCAACGG GCATGAGTTT 
GTTTTCCTCC TGAAGGGCCA CGAAGACCTG CGGCAGGATG AGCGGGTGAT GCAGCTCTTC 
GGCCTGGTGA ACACCCTTCT GGCCAATGAC CCAACGTCTC TTCGCAAGAA CCTCAGCATC 
CAGAGGTACG CTGTCATCCC CTTATCAACC AACTCGGGCC TCATTGGCTG GGTCCCCCAC 
TGCGACACGC TGCACGCCCT CATCCGGGAC TACAGAGAGA AAAAGAAGAT CCTTCTCAAC 
ATCGAGCATC GCATCATGTT GCGGATGGCT CCAGACTACG ATCACCTGAC GCTGATGCAG 
AAGGTGGAGG TGTTTGAGCA CGCCGTCAAC AACACAGCCG GGGACGACCT GGCCAAGCTG 
CTGTGGCTGA AAAGCCCTAG CTCCGAGGTA TGGTTTGACC GAAGAACCAA CTACACCCGC 
TCTTTAGCGG TCATGTCCAT GGTTGGGTAT ATTTTGGGCC TGGGAGATAG ACACCCATCC 
AATCTGATGC TTGACCGGCT GAGTGGGAAG ATCCTGCACA TCGACTTTGG GGACTGCTTT 
GAGGTTGCTA TGACCAGAGA GAAATTCCCA GAGAAGATTC CATTTAGACT AACGAGAATG 
CTGACCAATG CTATGGAGGT TACGGGTCTG GACGGCAACT ACAGAATCAC GTGCCACACG 
GTGATGGAGG TGCTTCGGGA ACACAAGGAC AGCGTCATGG CCGTGCTGGA AGCCTTTGTC 
TACGACCCCT TGCTGAACTG GAGGCTGATG GACACAAATA CCAAAGGCAA CAAGCGGTCC 
CGAACAAGGA CAGATTCCTA CTCTGCTGGC CAGTCAGTAG AAATTTTGGA TGGTGTGGAA 
CTTGGGGAAC CCGCCCACAA GAAAACAGGG ACCACAGTGC CGGAATCTAT CCATTCTTTC 
ATTGGAGACG GTTTGGTGAA GCCAGAGGCC CTAAATAAGA AAGCTATCCA GATAATTAAC 
AGGGTTCGAG ATAAGCTCAC TGGGCGGGAC TTTTCTCACG ATGAAACCCT GGATGTCCCG 
ACACAAGTCG AGCTGCTCAT CAAACAGGCC ACATCCCACG AGAACCTGTG TCAGTGCTAC 
ATCGGCTGGT GTCCTTTCTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MTOR cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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