Gene Symbol | PKLR |
Entrez Gene ID | 490425 |
Full Name | pyruvate kinase L/R |
General protein information |
|
Gene Type | protein-coding |
Organism | Canis lupus familiaris(dog) |
ORF » Species Summary » Canis lupus familiaris » PKLR cDNA ORF clone
gRNAs
Gene Symbol | PKLR |
Entrez Gene ID | 490425 |
Full Name | pyruvate kinase L/R |
General protein information |
|
Gene Type | protein-coding |
Organism | Canis lupus familiaris(dog) |
mRNA | Protein | Name |
---|---|---|
XM_005622262.2 | XP_005622319.1 | pyruvate kinase PKLR isoform X2 |
NM_001256018.1 | NP_001242947.1 | pyruvate kinase PKLR isoform 2 |
XM_022420764.1 | XP_022276472.1 | pyruvate kinase PKLR isoform X2 |
XM_022420765.1 | XP_022276473.1 | pyruvate kinase PKLR isoform X3 |
XM_022420766.1 | XP_022276474.1 | pyruvate kinase PKLR isoform X4 |
XM_014114980.1 | XP_013970455.1 | pyruvate kinase PKLR isoform X1 |
XM_022420763.1 | XP_022276471.1 | pyruvate kinase PKLR isoform X1 |
XM_014114980.2 | XP_013970455.1 | pyruvate kinase PKLR isoform X2 |
NM_001256262.1 | NP_001243191.1 | pyruvate kinase PKLR isoform 1 |
PKLR | XP_001112902.2 | |
Danio rerio (zebrafish) | pklr | NP_958446.1 |
Arabidopsis thaliana (thale cress) | AT3G55650 | NP_191124.1 |
Homo sapiens (human) | PKLR | NP_000289.1 |
Canis lupus familiaris (dog) | PKLR | NP_001243191.1 |
Bos taurus (cattle) | PKLR | NP_001069644.1 |
Mus musculus (house mouse) | Pklr | NP_038659.2 |
Saccharomyces cerevisiae (baker's yeast) | PYK2 | NP_014992.3 |
Arabidopsis thaliana (thale cress) | AT3G55810 | NP_191140.1 |
Rattus norvegicus (Norway rat) | Pklr | NP_036756.3 |
Arabidopsis thaliana (thale cress) | AT3G25960 | NP_189225.1 |
Pan troglodytes (chimpanzee) | PKLR | XP_524896.4 |
Arabidopsis thaliana (thale cress) | AT3G04050 | NP_187055.1 |
Erythrocytic pyruvate kinase mutations causing hemolytic anemia, osteosclerosis, and seconday hemochromatosis in dogs.
Gultekin GI, Raj K, Foureman P, Lehman S, Manhart K, Abdulmalik O, Giger U
Journal of veterinary internal medicine26(4)935-44(2012 Jul-Aug)
Chromosomal assignment of seven genes on canine chromosomes by fluorescence in situ hybridization.
Guevara-Fujita ML, Loechel R, Venta PJ, Yuzbasiyan-Gurkan V, Brewer GJ
Mammalian genome : official journal of the International Mammalian Genome Society7(4)268-70(1996 Apr)
The molecular basis of canine pyruvate kinase deficiency.
Whitney KM, Goodman SA, Bailey EM, Lothrop CD Jr
Experimental hematology22(9)866-74(1994 Aug)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Erythrocytic PK deficiency is caused by different mutations in different dog breeds and causes chronic severe hemolytic anemia, hemosiderosis, and secondary hemochromatosis because of chronic hemolysis and, an as yet unexplained osteosclerosis.
Title: Erythrocytic pyruvate kinase mutations causing hemolytic anemia, osteosclerosis, and seconday hemochromatosis in dogs.
The following PKLR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKLR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OCe00417 | |
Clone ID Related Accession (Same CDS sequence) | NM_001256018.1 | |
Accession Version | NM_001256018.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-21 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN338485.1 and AH004600.2. On Dec 23, 2011 this sequence version replaced XM_547547.2. Transcript Variant: This variant (2) uses an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Isoform 2 is also known as type L. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00013515, SAMN00013638 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## |
1 | ATGGAGGGAC CGGCGGGCTA CCTGAGGCGG GCCAGTGTGG CCCAGCTGAC CCTAGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001242947.1 |
CDS | 50..1681 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGAGGGAC CGGCGGGCTA CCTGAGGCGG GCCAGTGTGG CCCAGCTGAC CCTAGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe00422 | |
Clone ID Related Accession (Same CDS sequence) | NM_001256262.1 | |
Accession Version | NM_001256262.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-24 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03005338.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Isoform 1 is also known as type R. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. There are no full-length transcripts supporting this RefSeq in dog; it is predicted based on partial transcript alignments and on full-length transcript support from the human homolog (GeneID: 5313, NM_000298.5 and AH004600.2). However, it is unknown if this exon combination exists in dog. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA4547738, SAMEA4547743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## |
1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001243191.1 |
CDS | 1..1725 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe08958 | |
Clone ID Related Accession (Same CDS sequence) | XM_005622262.2 | |
Accession Version | XM_005622262.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-09-16 | |
Organism | Canis lupus familiaris(Dog) | |
Product | pyruvate kinase PKLR isoform X2 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005622262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGAGGGAC CGGCGGGCTA CCTGAGGCGG GCCAGTGTGG CCCAGCTGAC CCTAGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005622319.1 |
CDS | 98..1576 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGAGGGAC CGGCGGGCTA CCTGAGGCGG GCCAGTGTGG CCCAGCTGAC CCTAGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe289397 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420765.1 | |
Accession Version | XM_022420765.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-09-04 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGATCAAGG CCGGGATGAA CATTGCGCGC CTCAACTTCT CCCACGGCTC CCATGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_022276473.1 |
CDS | 79..1485 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGATCAAGG CCGGGATGAA CATTGCGCGC CTCAACTTCT CCCACGGCTC CCATGAGTAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe08957 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420766.1 | |
Accession Version | XM_022420766.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-09-04 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_022276474.1 |
CDS | 46..1812 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe289396 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420763.1 | |
Accession Version | XM_022420763.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-09-04 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_022276471.1 |
CDS | 46..1617 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCCAGTC AGGAGAACAT ACAACCCCAG GAGCTTTGGT CAAGGATCTC TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe37329 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420764.1 , XM_014114980.1 , XM_014114980.2 | |
Accession Version | XM_022420764.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-09-04 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_022276472.1 |
CDS | 167..1699 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe37329 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420764.1 , XM_014114980.1 , XM_014114980.2 | |
Accession Version | XM_014114980.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-09-16 | |
Organism | Canis lupus familiaris(Dog) | |
Product | pyruvate kinase PKLR isoform X1 | |
Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_013970455.1 |
CDS | 268..1800 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OCe37329 | |
Clone ID Related Accession (Same CDS sequence) | XM_022420764.1 , XM_014114980.1 , XM_014114980.2 | |
Accession Version | XM_014114980.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2017-09-04 | |
Organism | Canis lupus familiaris(dog) | |
Product | pyruvate kinase PKLR isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014114980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_013970455.1 |
CDS | 290..1822 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCAGACA CCTTCCTGGA GCACCTGTGC CTGCTGGACA TCGACTCGGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Erythrocytic pyruvate kinase mutations causing hemolytic anemia, osteosclerosis, and seconday hemochromatosis in dogs. |
Chromosomal assignment of seven genes on canine chromosomes by fluorescence in situ hybridization. |
The molecular basis of canine pyruvate kinase deficiency. |