Gene Symbol | Pklr |
Entrez Gene ID | 18770 |
Full Name | pyruvate kinase liver and red blood cell |
Synonyms | Pk-1,Pk1,R-PK |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
ORF » Species Summary » Mus musculus » Pklr cDNA ORF clone
gRNAs
Gene Symbol | Pklr |
Entrez Gene ID | 18770 |
Full Name | pyruvate kinase liver and red blood cell |
Synonyms | Pk-1,Pk1,R-PK |
General protein information |
|
Gene Type | protein-coding |
Organism | Mus musculus(house mouse) |
Genome |
mRNA | Protein | Name |
---|---|---|
XM_006501134.4 | XP_006501197.1 | pyruvate kinase PKLR isoform X1 |
XM_006501135.4 | XP_006501198.1 | pyruvate kinase PKLR isoform X2 |
XM_006501134.3 | XP_006501197.1 | pyruvate kinase PKLR isoform X1 |
XM_006501135.3 | XP_006501198.1 | pyruvate kinase PKLR isoform X2 |
NM_013631.2 | NP_038659.2 | pyruvate kinase PKLR isoform 1 |
NM_001099779.1 | NP_001093249.1 | pyruvate kinase PKLR isoform 2 |
PKLR | XP_001112902.2 | |
Danio rerio (zebrafish) | pklr | NP_958446.1 |
Arabidopsis thaliana (thale cress) | AT3G55650 | NP_191124.1 |
Homo sapiens (human) | PKLR | NP_000289.1 |
Canis lupus familiaris (dog) | PKLR | NP_001243191.1 |
Bos taurus (cattle) | PKLR | NP_001069644.1 |
Mus musculus (house mouse) | Pklr | NP_038659.2 |
Saccharomyces cerevisiae (baker's yeast) | PYK2 | NP_014992.3 |
Arabidopsis thaliana (thale cress) | AT3G55810 | NP_191140.1 |
Rattus norvegicus (Norway rat) | Pklr | NP_036756.3 |
Arabidopsis thaliana (thale cress) | AT3G25960 | NP_189225.1 |
Pan troglodytes (chimpanzee) | PKLR | XP_524896.4 |
Arabidopsis thaliana (thale cress) | AT3G04050 | NP_187055.1 |
Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)
The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria.
Laroque A, Min-Oo G, Tam M, Ponka P, Stevenson MM, Gros P
PloS one12(5)e0177818(2017)
Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)
Germline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma.
Dayton TL, Gocheva V, Miller KM, Israelsen WJ, Bhutkar A, Clish CB, Davidson SM, Luengo A, Bronson RT, Jacks T, Vander Heiden MG
Genes & development30(9)1020-33(2016 05)
Survival analysis and microarray profiling identify Cd40 as a candidate for the Salmonella susceptibility locus, Ity5.
Beatty SC, Yuki KE, Eva MM, Dauphinee S, Larivi?re L, Vidal SM, Malo D
Genes and immunity17(1)19-29(2016 Jan-Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
AMPD3 deficiency increases the level of ATP in erythrocytes, but does not improve anemia due to pyruvate kinase deficiency and leads to erythrocyte dysfunction.
Title: AMPD3-deficient mice exhibit increased erythrocyte ATP levels but anemia not improved due to PK deficiency.
the metabolic disturbance in PK deficiency alters not only the survival of red cells but also the maturation of erythroid progenitors, resulting in ineffective erythropoiesis.
Title: Ineffective erythropoiesis in mutant mice with deficient pyruvate kinase activity.
Pyruvate kinase deficiency in mice protects against malaria
Title: Pyruvate kinase deficiency in mice protects against malaria.
The following Pklr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pklr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OMu14963 | |
Clone ID Related Accession (Same CDS sequence) | NM_013631.2 | |
Accession Version | NM_013631.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-25 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform 1 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY781095.1, BY107231.1, AK149508.1 and AK149524.1. On Jul 25, 2007 this sequence version replaced NM_013631.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Isoform 1 is also known as isoform R. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63764.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## |
1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_038659.2 |
CDS | 42..1766 |
Translation |
Target ORF information:
Target ORF information:
|
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1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31696 | |
Clone ID Related Accession (Same CDS sequence) | XM_006501134.4 , XM_006501134.3 | |
Accession Version | XM_006501134.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501134.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCAGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006501197.1 |
CDS | 195..1727 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCAGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu15199 | |
Clone ID Related Accession (Same CDS sequence) | NM_001099779.1 | |
Accession Version | NM_001099779.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-19 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform 2 | |
Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK149508.1 and AK149524.1. Transcript Variant: This variant (2) differs in the 5' end-region, which includes a part of the coding region, as compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Isoform 2 is also known as isoform L. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK149524.1, AK149508.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## |
1 | ATGGAAGGGC CAGCAGGATA CCTGAGACGT GCCAGTGTGG CCCAGCTGAC CCAGGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001093249.1 |
CDS | 9..1640 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGAAGGGC CAGCAGGATA CCTGAGACGT GCCAGTGTGG CCCAGCTGAC CCAGGAGCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31697 | |
Clone ID Related Accession (Same CDS sequence) | XM_006501135.4 , XM_006501135.3 | |
Accession Version | XM_006501135.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-21 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006501198.1 |
CDS | 55..1851 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31696 | |
Clone ID Related Accession (Same CDS sequence) | XM_006501134.4 , XM_006501134.3 | |
Accession Version | XM_006501134.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501134.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCAGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006501197.1 |
CDS | 195..1727 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCAGA GCCTGTGGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OMu31697 | |
Clone ID Related Accession (Same CDS sequence) | XM_006501135.4 , XM_006501135.3 | |
Accession Version | XM_006501135.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-07 | |
Organism | Mus musculus(house mouse) | |
Product | pyruvate kinase PKLR isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_006501198.1 |
CDS | 62..1858 |
Translation |
Target ORF information:
Target ORF information:
|
![]() |
1 | ATGTCTGTCC AAGAGAACGA GCTACCCCAG CAGCTCTGGC CCTGGATCTT TAAGTCCCAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development. |
The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria. |
Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series. |
Germline loss of PKM2 promotes metabolic distress and hepatocellular carcinoma. |
Survival analysis and microarray profiling identify Cd40 as a candidate for the Salmonella susceptibility locus, Ity5. |