COL6A6 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A6
Entrez Gene ID	610649
Full Name	collagen type VI alpha 6 chain
General protein information	Preferred Names collagen type VI alpha 6 chain Names collagen alpha-6(VI) chain
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022408620.1	XP_022264328.1	collagen alpha-6(VI) chain isoform X3
XM_848186.5	XP_853279.3	collagen alpha-6(VI) chain isoform X1
XM_022408619.1	XP_022264327.1	collagen alpha-6(VI) chain isoform X2
XM_848186.4	XP_853279.3	collagen alpha-6(VI) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL6A6	XP_003310057.1
Bos taurus (cattle)	COL6A6	XP_002685218.2
Gallus gallus (chicken)	COL6A6	XP_426008.4
Xenopus tropicalis (tropical clawed frog)	LOC100486063	XP_002942405.2
Canis lupus familiaris (dog)	COL6A6	XP_853279.3
Mus musculus (house mouse)	Col6a6	NP_001096077.1
	COL6A6	XP_001113364.2
Homo sapiens (human)	COL6A6	NP_001096078.1
Rattus norvegicus (Norway rat)	Col6a6	XP_236593.5
Danio rerio (zebrafish)	col6a6	XP_692457.6

Gene Ontology

The following COL6A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296494	XM_022408619.1 Latest version!	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe296495	XM_022408620.1 Latest version!	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe24130	XM_848186.5 Latest version!	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe24130	XM_848186.4	Canis lupus familiaris collagen, type VI, alpha 6 (COL6A6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A6 ( XM_022408619.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264327.1 Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.

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CloneID	OCe296494
Clone ID Related Accession (Same CDS sequence)	XM_022408619.1
Accession Version	XM_022408619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-6(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGA 
TTCTTGCAGG TAATGTTTGA CCCAAGTCTC ATAATGAGGA AACCATTAGC CAATTCCAGA 
ATGTGGATTT CTTCAGAACA GCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264327.1
CDS	135..6824
Translation	MKMLLILFLIIICSPVSMNKDSGPEYADVVFLVDSSDHLGAKSFPLVKTFINRMISSLPIEAHKYRVALAQYSDQLHSEFQLGTFKSRNPMLNHLKKNFGFVGGSLRIGQALREAHRTYFSRPDSGRDKKQFPPILVVLASAESEDDVEEPSKALRGDGVRIISVGLQSASEQELKAMATGQFHFNLRSARDLGAFAPNMTQIIKEATQYREVATDDILVEVCQGPSVADLVFLLDVSVNGSQENFDYLKEFLEESVSALDIKENCMRVGLVTYSNETKVINSLSRGVNKSEVLQNIQNLSPRAGKAYTGAAIRKIRKEVFSARNGSRKNQGVPQIAVLVTHRPSEDNVTKAAVNLRREGVTIFTMGIEGASDSQLEKIASHPAEQHVSKLKTFSELAAHNQTFLKKLRNQITLTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPQKVRVGAVQYADSWDLEFEINKYTNKHDLGKAIENIRQMGGNTNTGAALNFTLGLLQKAKKQRGNRVPCHLVVLTNGMSKDSILEPANRLREELIRVYAIGVKEANQTQLREIAGEDKRVYYVHDFDALKDIRNQVVQEICAEEACKEMKADIMFLVDSSGSIGLENFIKMKTFMKNLVSKSQIGADRVQIGVVQFSDINKEEFQLNRYMSQNEISNAIDRMAHIGETTLTGSALTFVSQYFSPAKGARPNVRRFLILITDGEAQDIVKDPAVALRQEGIIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQHIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDHDEYNIMKDFMVDLVKKADVGKNQVRFGALKYADDPEVLFYLDDLSTKWEVISVLQKDQPMGGNTYTAEALGFSDHMFTEARGSRLHKGVPQVLIVITDGESHDADKLNDTAKALRDKGILVLAVGIAGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFKKMKEFLASVVQDFDVSVNRVRIGAAQFSHTYRPEFPLGTFIGKKEISFQIENIQQIFGYTHIGAALREVGDYFRPDMGSRINAGTPQVLLVLTDGQSQDEVAQAAEELRHKGIDIYSVGIGDVDDQQLIQITGTADKKLTVHNFDELTKVKKRIVRNICTSGGESNCFVDVVVGFDMSTHENGQTLLEGQSWIETYLQDLLRAISSLNGVSCEVGTETQVSVAFQVTNAVEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLSSLWDAFQNKSAARGKVVLLFSDGLDDDTEKLEQKSDELRKEGLNALITVALDGATDSSDLADLLYIEFGKGFEYRTQLTIGMRDLGSRLSKLLVNVAERTCCCLFCKCIGGDGTMRDPGPPGKKGSPGFKGSEGYLGEEGVAGDRGASGPMGEQGTKGCYGAKGPKGNRGLNGQEGEVGESGIGGLNGEPGDSGLPGEKGEKGNEGSQGSPGKRGVSGDRGSKGQRGDPGVPGFDNTIEGPKGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGKTGIPGPDGLEGSWGVKGPQGPRGETGVKGEKGGLGSKGPQGPPGQGGEAGSPGHLGSQGTKGEPGDLGEKGAIGLPGPRGLPGDDGNPGYGSIGRKGAKGQDGFPGESGPKGEMGDLGGPGETGPKGARGKRISTGLPGEMGSPGEPGPPGRKGVKGARGLASFSMCELIQYVRDHSPGGHGKPECPVHPTELVFALDQSRDVTKQEFERMKELMSSLVRGVRVRENSCPVGARIAILTYTSHARHLVRFSDAYKKQQLLREIEAIPYERSSDSREIGGVMRFISRNVFKRTLPGSHTRRIATFFSSGQSADAQTIATAAMEFSALDIVPVVIAFSNVPSIKRAFAIDDTGTFQVIMVPSGTDYAPALERLQRCTFCYDVCKPDTFCDEAKPPPAQSYMDAAFLLDSSRHVQSAEFEDMRHFLGALLDHFEISPEPETSVTGDRVALLSHAPPNFLPNTQKSPVKTEFNLTTYNSKRLMKRHMEQSVQQLNGDAFIGHALQWTLDNIFLNTPNMRRNKVIFVISAGETNHLDRETLKKESLRAKCQGYALFVFSLGPAWNDKELEDLASYPLDHHLVQLGRIHKPDHRYSVNFVKSFVNSIRRAINKYPPISFKMKCNRLTSTDVKQPPRQWRRFLQVMFDPSLIMRKPLANSRMWISSEQQT

Target ORF information:

RefSeq Version	XM_022408619.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408619.1

ORF Insert Sequence:

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGA 
TTCTTGCAGG TAATGTTTGA CCCAAGTCTC ATAATGAGGA AACCATTAGC CAATTCCAGA 
ATGTGGATTT CTTCAGAACA GCAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_022408620.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022264328.1 Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X3, mRNA.

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CloneID	OCe296495
Clone ID Related Accession (Same CDS sequence)	XM_022408620.1
Accession Version	XM_022408620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-6(VI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264328.1
CDS	135..6737
Translation	MKMLLILFLIIICSPVSMNKDSGPEYADVVFLVDSSDHLGAKSFPLVKTFINRMISSLPIEAHKYRVALAQYSDQLHSEFQLGTFKSRNPMLNHLKKNFGFVGGSLRIGQALREAHRTYFSRPDSGRDKKQFPPILVVLASAESEDDVEEPSKALRGDGVRIISVGLQSASEQELKAMATGQFHFNLRSARDLGAFAPNMTQIIKEATQYREVATDDILVEVCQGPSVADLVFLLDVSVNGSQENFDYLKEFLEESVSALDIKENCMRVGLVTYSNETKVINSLSRGVNKSEVLQNIQNLSPRAGKAYTGAAIRKIRKEVFSARNGSRKNQGVPQIAVLVTHRPSEDNVTKAAVNLRREGVTIFTMGIEGASDSQLEKIASHPAEQHVSKLKTFSELAAHNQTFLKKLRNQITLTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPQKVRVGAVQYADSWDLEFEINKYTNKHDLGKAIENIRQMGGNTNTGAALNFTLGLLQKAKKQRGNRVPCHLVVLTNGMSKDSILEPANRLREELIRVYAIGVKEANQTQLREIAGEDKRVYYVHDFDALKDIRNQVVQEICAEEACKEMKADIMFLVDSSGSIGLENFIKMKTFMKNLVSKSQIGADRVQIGVVQFSDINKEEFQLNRYMSQNEISNAIDRMAHIGETTLTGSALTFVSQYFSPAKGARPNVRRFLILITDGEAQDIVKDPAVALRQEGIIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQHIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDHDEYNIMKDFMVDLVKKADVGKNQVRFGALKYADDPEVLFYLDDLSTKWEVISVLQKDQPMGGNTYTAEALGFSDHMFTEARGSRLHKGVPQVLIVITDGESHDADKLNDTAKALRDKGILVLAVGIAGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFKKMKEFLASVVQDFDVSVNRVRIGAAQFSHTYRPEFPLGTFIGKKEISFQIENIQQIFGYTHIGAALREVGDYFRPDMGSRINAGTPQVLLVLTDGQSQDEVAQAAEELRHKGIDIYSVGIGDVDDQQLIQITGTADKKLTVHNFDELTKVKKRIVRNICTSGGESNCFVDVVVGFDMSTHENGQTLLEGQSWIETYLQDLLRAISSLNGVSCEVGTETQVSVAFQVTNAVEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLSSLWDAFQNKSAARGKVVLLFSDGLDDDTEKLEQKSDELRKEGLNALITVALDGATDSSDLADLLYIEFGKGFEYRTQLTIGMRDLGSRLSKLLVNVAERTCCCLFCKCIGGDGTMRDPGPPGKKGSPGFKGSEGYLGEEGVAGDRGASGPMGEQGTKGCYGAKGPKGNRGLNGQEGEVGESGIGGLNGEPGDSGLPGEKGEKGNEGSQGSPGKRGVSGDRGSKGQRGDPGVPGFDNTIEGPKGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGKTGIPGPDGLEGSWGVKGPQGPRGETGVKGEKGGLGSKGPQGPPGQGGEAGSPGHLGSQGTKGEPGDLGEKGAIGLPGPRGLPGDDGNPGYGSIGRKGAKGQDGFPGESGPKGEMGDLGGPGETGPKGARGKRISTGLPGEMGSPGEPGPPGRKGVKGARGLASFSMCELIQYVRDHSPGGHGKPECPVHPTELVFALDQSRDVTKQEFERMKELMSSLVRGVRVRENSCPVGARIAILTYTSHARHLVRFSDAYKKQQLLREIEAIPYERSSDSREIGGVMRFISRNVFKRTLPGSHTRRIATFFSSGQSADAQTIATAAMEFSALDIVPVVIAFSNVPSIKRAFAIDDTGTFQVIMVPSGTDYAPALERLQRCTFCYDVCKPDTFCDEAKPPPAQSYMDAAFLLDSSRHVQSAEFEDMRHFLGALLDHFEISPEPETSVTGDRVALLSHAPPNFLPNTQKSPVKTEFNLTTYNSKRLMKRHMEQSVQQLNGDAFIGHALQWTLDNIFLNTPNMRRNKVIFVISAGETNHLDRETLKKESLRAKCQGYALFVFSLGPAWNDKELEDLASYPLDHHLVQLGRIHKPDHRYSVNFVKSFVNSIRRAINKYPPISFKMKCNRLTSTDVKQPPRQWRR

Target ORF information:

RefSeq Version	XM_022408620.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022408620.1

ORF Insert Sequence:

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_848186.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_853279.3 Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.

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CloneID	OCe24130
Clone ID Related Accession (Same CDS sequence)	XM_848186.5 , XM_848186.4
Accession Version	XM_848186.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-6(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006605.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_848186.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGC 
TTTGTTCCTG GACCACATAA AACTGCCCTC AAAGAAGATG CATTACAGAA GGCAAAGTTC 
TTTCAAGATA AAAAATATCT TTCAAGAGTA GCAAGAGGCA GCAGAGATAT TATTCGAAAT 
CTTACCAGAA ACACATTCCA TACCTTTAAA AATGGAAAAA AGGTGATAAA AACTGCTCCA 
AAACAACATG ATAAAGCAAG TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853279.3
CDS	134..6940
Translation	MKMLLILFLIIICSPVSMNKDSGPEYADVVFLVDSSDHLGAKSFPLVKTFINRMISSLPIEAHKYRVALAQYSDQLHSEFQLGTFKSRNPMLNHLKKNFGFVGGSLRIGQALREAHRTYFSRPDSGRDKKQFPPILVVLASAESEDDVEEPSKALRGDGVRIISVGLQSASEQELKAMATGQFHFNLRSARDLGAFAPNMTQIIKEATQYREVATDDILVEVCQGPSVADLVFLLDVSVNGSQENFDYLKEFLEESVSALDIKENCMRVGLVTYSNETKVINSLSRGVNKSEVLQNIQNLSPRAGKAYTGAAIRKIRKEVFSARNGSRKNQGVPQIAVLVTHRPSEDNVTKAAVNLRREGVTIFTMGIEGASDSQLEKIASHPAEQHVSKLKTFSELAAHNQTFLKKLRNQITLTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPQKVRVGAVQYADSWDLEFEINKYTNKHDLGKAIENIRQMGGNTNTGAALNFTLGLLQKAKKQRGNRVPCHLVVLTNGMSKDSILEPANRLREELIRVYAIGVKEANQTQLREIAGEDKRVYYVHDFDALKDIRNQVVQEICAEEACKEMKADIMFLVDSSGSIGLENFIKMKTFMKNLVSKSQIGADRVQIGVVQFSDINKEEFQLNRYMSQNEISNAIDRMAHIGETTLTGSALTFVSQYFSPAKGARPNVRRFLILITDGEAQDIVKDPAVALRQEGIIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQHIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDHDEYNIMKDFMVDLVKKADVGKNQVRFGALKYADDPEVLFYLDDLSTKWEVISVLQKDQPMGGNTYTAEALGFSDHMFTEARGSRLHKGVPQVLIVITDGESHDADKLNDTAKALRDKGILVLAVGIAGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFKKMKEFLASVVQDFDVSVNRVRIGAAQFSHTYRPEFPLGTFIGKKEISFQIENIQQIFGYTHIGAALREVGDYFRPDMGSRINAGTPQVLLVLTDGQSQDEVAQAAEELRHKGIDIYSVGIGDVDDQQLIQITGTADKKLTVHNFDELTKVKKRIVRNICTSGGESNCFVDVVVGFDMSTHENGQTLLEGQSWIETYLQDLLRAISSLNGVSCEVGTETQVSVAFQVTNAVEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLSSLWDAFQNKSAARGKVVLLFSDGLDDDTEKLEQKSDELRKEGLNALITVALDGATDSSDLADLLYIEFGKGFEYRTQLTIGMRDLGSRLSKLLVNVAERTCCCLFCKCIGGDGTMRDPGPPGKKGSPGFKGSEGYLGEEGVAGDRGASGPMGEQGTKGCYGAKGPKGNRGLNGQEGEVGESGIGGLNGEPGDSGLPGEKGEKGNEGSQGSPGKRGVSGDRGSKGQRGDPGVPGFDNTIEGPKGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGKTGIPGPDGLEGSWGVKGPQGPRGETGVKGEKGGLGSKGPQGPPGQGGEAGSPGHLGSQGTKGEPGDLGEKGAIGLPGPRGLPGDDGNPGYGSIGRKGAKGQDGFPGESGPKGEMGDLGGPGETGPKGARGKRISTGLPGEMGSPGEPGPPGRKGVKGARGLASFSMCELIQYVRDHSPGGHGKPECPVHPTELVFALDQSRDVTKQEFERMKELMSSLVRGVRVRENSCPVGARIAILTYTSHARHLVRFSDAYKKQQLLREIEAIPYERSSDSREIGGVMRFISRNVFKRTLPGSHTRRIATFFSSGQSADAQTIATAAMEFSALDIVPVVIAFSNVPSIKRAFAIDDTGTFQVIMVPSGTDYAPALERLQRCTFCYDVCKPDTFCDEAKPPPAQSYMDAAFLLDSSRHVQSAEFEDMRHFLGALLDHFEISPEPETSVTGDRVALLSHAPPNFLPNTQKSPVKTEFNLTTYNSKRLMKRHMEQSVQQLNGDAFIGHALQWTLDNIFLNTPNMRRNKVIFVISAGETNHLDRETLKKESLRAKCQGYALFVFSLGPAWNDKELEDLASYPLDHHLVQLGRIHKPDHRYSVNFVKSFVNSIRRAINKYPPISFKMKCNRLTSTDVKQPPRQWRSFVPGPHKTALKEDALQKAKFFQDKKYLSRVARGSRDIIRNLTRNTFHTFKNGKKVIKTAPKQHDKASA

Target ORF information:

RefSeq Version	XM_848186.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type VI alpha 6 chain (COL6A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_848186.5

ORF Insert Sequence:

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGC 
TTTGTTCCTG GACCACATAA AACTGCCCTC AAAGAAGATG CATTACAGAA GGCAAAGTTC 
TTTCAAGATA AAAAATATCT TTCAAGAGTA GCAAGAGGCA GCAGAGATAT TATTCGAAAT 
CTTACCAGAA ACACATTCCA TACCTTTAAA AATGGAAAAA AGGTGATAAA AACTGCTCCA 
AAACAACATG ATAAAGCAAG TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A6 ( XM_848186.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_853279.3 Canis lupus familiaris collagen, type VI, alpha 6 (COL6A6), mRNA.

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CloneID	OCe24130
Clone ID Related Accession (Same CDS sequence)	XM_848186.5 , XM_848186.4
Accession Version	XM_848186.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	collagen alpha-6(VI) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726100.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_848186.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGC 
TTTGTTCCTG GACCACATAA AACTGCCCTC AAAGAAGATG CATTACAGAA GGCAAAGTTC 
TTTCAAGATA AAAAATATCT TTCAAGAGTA GCAAGAGGCA GCAGAGATAT TATTCGAAAT 
CTTACCAGAA ACACATTCCA TACCTTTAAA AATGGAAAAA AGGTGATAAA AACTGCTCCA 
AAACAACATG ATAAAGCAAG TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_853279.3
CDS	228..7034
Translation	MKMLLILFLIIICSPVSMNKDSGPEYADVVFLVDSSDHLGAKSFPLVKTFINRMISSLPIEAHKYRVALAQYSDQLHSEFQLGTFKSRNPMLNHLKKNFGFVGGSLRIGQALREAHRTYFSRPDSGRDKKQFPPILVVLASAESEDDVEEPSKALRGDGVRIISVGLQSASEQELKAMATGQFHFNLRSARDLGAFAPNMTQIIKEATQYREVATDDILVEVCQGPSVADLVFLLDVSVNGSQENFDYLKEFLEESVSALDIKENCMRVGLVTYSNETKVINSLSRGVNKSEVLQNIQNLSPRAGKAYTGAAIRKIRKEVFSARNGSRKNQGVPQIAVLVTHRPSEDNVTKAAVNLRREGVTIFTMGIEGASDSQLEKIASHPAEQHVSKLKTFSELAAHNQTFLKKLRNQITLTVSVFSERTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPQKVRVGAVQYADSWDLEFEINKYTNKHDLGKAIENIRQMGGNTNTGAALNFTLGLLQKAKKQRGNRVPCHLVVLTNGMSKDSILEPANRLREELIRVYAIGVKEANQTQLREIAGEDKRVYYVHDFDALKDIRNQVVQEICAEEACKEMKADIMFLVDSSGSIGLENFIKMKTFMKNLVSKSQIGADRVQIGVVQFSDINKEEFQLNRYMSQNEISNAIDRMAHIGETTLTGSALTFVSQYFSPAKGARPNVRRFLILITDGEAQDIVKDPAVALRQEGIIIYSVGVFGSNVTQLEEISGRPEMVFYVENFDILQHIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDHDEYNIMKDFMVDLVKKADVGKNQVRFGALKYADDPEVLFYLDDLSTKWEVISVLQKDQPMGGNTYTAEALGFSDHMFTEARGSRLHKGVPQVLIVITDGESHDADKLNDTAKALRDKGILVLAVGIAGANPVELLAMAGSSDKYFFVETFGGLKGIFSDVSASVCNSSKVDCEIEKVDLVFLMDGSNSIHPDDFKKMKEFLASVVQDFDVSVNRVRIGAAQFSHTYRPEFPLGTFIGKKEISFQIENIQQIFGYTHIGAALREVGDYFRPDMGSRINAGTPQVLLVLTDGQSQDEVAQAAEELRHKGIDIYSVGIGDVDDQQLIQITGTADKKLTVHNFDELTKVKKRIVRNICTSGGESNCFVDVVVGFDMSTHENGQTLLEGQSWIETYLQDLLRAISSLNGVSCEVGTETQVSVAFQVTNAVEKYSPKFEIYSENILNSLKDITVKGPSLLNANLLSSLWDAFQNKSAARGKVVLLFSDGLDDDTEKLEQKSDELRKEGLNALITVALDGATDSSDLADLLYIEFGKGFEYRTQLTIGMRDLGSRLSKLLVNVAERTCCCLFCKCIGGDGTMRDPGPPGKKGSPGFKGSEGYLGEEGVAGDRGASGPMGEQGTKGCYGAKGPKGNRGLNGQEGEVGESGIGGLNGEPGDSGLPGEKGEKGNEGSQGSPGKRGVSGDRGSKGQRGDPGVPGFDNTIEGPKGLKGERGRQGRRGWPGPPGTPGSRRKTAAHGRRGHTGPQGKTGIPGPDGLEGSWGVKGPQGPRGETGVKGEKGGLGSKGPQGPPGQGGEAGSPGHLGSQGTKGEPGDLGEKGAIGLPGPRGLPGDDGNPGYGSIGRKGAKGQDGFPGESGPKGEMGDLGGPGETGPKGARGKRISTGLPGEMGSPGEPGPPGRKGVKGARGLASFSMCELIQYVRDHSPGGHGKPECPVHPTELVFALDQSRDVTKQEFERMKELMSSLVRGVRVRENSCPVGARIAILTYTSHARHLVRFSDAYKKQQLLREIEAIPYERSSDSREIGGVMRFISRNVFKRTLPGSHTRRIATFFSSGQSADAQTIATAAMEFSALDIVPVVIAFSNVPSIKRAFAIDDTGTFQVIMVPSGTDYAPALERLQRCTFCYDVCKPDTFCDEAKPPPAQSYMDAAFLLDSSRHVQSAEFEDMRHFLGALLDHFEISPEPETSVTGDRVALLSHAPPNFLPNTQKSPVKTEFNLTTYNSKRLMKRHMEQSVQQLNGDAFIGHALQWTLDNIFLNTPNMRRNKVIFVISAGETNHLDRETLKKESLRAKCQGYALFVFSLGPAWNDKELEDLASYPLDHHLVQLGRIHKPDHRYSVNFVKSFVNSIRRAINKYPPISFKMKCNRLTSTDVKQPPRQWRSFVPGPHKTALKEDALQKAKFFQDKKYLSRVARGSRDIIRNLTRNTFHTFKNGKKVIKTAPKQHDKASA

Target ORF information:

RefSeq Version	XM_848186.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris collagen, type VI, alpha 6 (COL6A6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_848186.4

ORF Insert Sequence:

ATGAAAATGC TGCTGATTTT ATTCCTCATA ATAATTTGTT CTCCTGTCTC TATGAACAAA 
GATTCTGGTC CTGAGTATGC AGATGTCGTG TTTCTGGTGG ACAGCTCCGA TCACCTGGGA 
GCTAAGTCCT TCCCTCTTGT GAAAACATTC ATCAACAGAA TGATCAGCAG CCTCCCCATC 
GAGGCCCACA AGTACCGCGT GGCCCTGGCC CAGTACAGCG ACCAGCTCCA CAGTGAGTTC 
CAGCTGGGCA CGTTCAAGAG CAGGAACCCC ATGCTGAACC ACCTCAAGAA GAACTTCGGC 
TTCGTTGGTG GCTCCCTGCG CATAGGCCAA GCCCTCCGGG AGGCCCACAG GACCTACTTC 
TCCAGGCCCG ACAGCGGGAG GGACAAGAAG CAGTTTCCCC CGATTCTGGT GGTCCTGGCT 
TCGGCCGAGT CCGAGGATGA CGTGGAGGAG CCCTCGAAGG CCCTGCGGGG AGATGGAGTG 
AGAATCATCT CCGTGGGGCT GCAGAGCGCG TCTGAGCAGG AGCTGAAGGC CATGGCCACG 
GGGCAGTTTC ATTTCAACCT GCGGTCGGCC AGGGACCTTG GAGCGTTTGC CCCAAACATG 
ACGCAGATCA TCAAGGAAGC CACCCAGTAC AGGGAGGTAG CCACCGATGA CATTCTTGTG 
GAAGTTTGCC AAGGCCCGTC TGTGGCTGAC TTGGTGTTCC TGTTGGATGT GTCCGTCAAC 
GGCAGCCAGG AGAACTTTGA CTATCTTAAG GAATTCCTGG AAGAGAGTGT ATCTGCCCTT 
GACATAAAGG AAAACTGCAT GAGGGTTGGC CTGGTGACCT ACAGCAATGA GACGAAGGTG 
ATAAACTCAC TCAGCAGGGG CGTAAATAAG TCGGAGGTTC TCCAGAATAT ACAGAACCTC 
TCTCCCCGGG CCGGGAAGGC CTACACAGGG GCTGCCATCA GAAAAATCAG GAAGGAAGTG 
TTCAGTGCAC GCAACGGCAG TCGGAAGAAT CAGGGGGTGC CCCAGATTGC GGTGCTGGTG 
ACCCACAGGC CCTCAGAGGA TAACGTGACC AAGGCAGCTG TTAACCTCCG GCGCGAGGGG 
GTGACCATCT TCACCATGGG CATAGAGGGG GCCAGCGACA GCCAGCTGGA GAAGATCGCC 
TCTCACCCTG CCGAGCAGCA CGTGTCCAAA CTGAAAACCT TCTCCGAGCT GGCCGCGCAC 
AACCAGACGT TCCTGAAGAA GCTGCGGAAC CAGATCACGC TCACTGTCTC TGTCTTCTCG 
GAACGCACTG AAACGCTCAA ATCCGGTTGT GTGGATACTG AAGAAGCAGA CATCTATCTG 
CTCATTGATG GCTCTGGAAG CACCCAGGCC ACAGACTTCC ACGAAATGAA GACCTTCCTG 
TCCGAGGTTG TAGGAATGTT CAACATTGCA CCCCAAAAGG TGCGGGTTGG GGCTGTTCAG 
TATGCGGACA GCTGGGACCT GGAATTTGAG ATCAATAAAT ACACTAATAA GCATGACTTG 
GGAAAGGCCA TTGAGAACAT TCGGCAGATG GGTGGGAACA CAAACACGGG TGCAGCCCTA 
AATTTCACAC TAGGGCTGTT GCAAAAAGCA AAGAAGCAAC GAGGAAACAG AGTGCCCTGT 
CATCTTGTTG TCCTGACGAA TGGCATGTCC AAGGATAGCA TCTTAGAGCC TGCAAACAGA 
CTGAGAGAAG AACTCATTCG CGTTTATGCC ATCGGGGTCA AGGAGGCCAA CCAAACACAG 
CTGCGAGAAA TTGCAGGCGA GGACAAGAGG GTGTACTATG TGCATGACTT TGATGCTTTA 
AAAGACATAA GAAACCAAGT TGTTCAAGAA ATCTGTGCTG AAGAAGCATG CAAAGAGATG 
AAAGCAGACA TCATGTTTCT GGTGGACAGT TCTGGCAGTA TAGGACTTGA AAACTTCATC 
AAAATGAAAA CATTTATGAA AAACCTGGTG AGCAAATCTC AGATTGGGGC AGATCGAGTG 
CAGATCGGCG TAGTCCAGTT CAGTGACATC AATAAGGAGG AATTCCAGCT CAACAGATAT 
ATGTCCCAAA ACGAAATTTC AAATGCAATA GACCGGATGG CTCACATTGG GGAAACCACC 
TTGACTGGTA GTGCCCTGAC CTTCGTTTCT CAGTACTTCA GCCCTGCCAA AGGGGCCCGG 
CCCAATGTCA GGAGGTTTCT CATCCTCATC ACTGACGGTG AAGCTCAGGA CATAGTCAAG 
GACCCAGCAG TAGCACTTCG ACAAGAAGGC ATAATTATCT ACTCTGTGGG GGTGTTTGGT 
TCCAATGTCA CTCAGCTCGA GGAAATCAGC GGGAGGCCGG AGATGGTTTT TTATGTTGAG 
AATTTTGATA TTCTGCAACA CATTGAAGAT GATCTTGTTT TTGGAATATG CAGCCCCCGT 
GAAGAATGCA AGCGGATTGA GGTTTTGGAT GTTGTGTTTG TAATTGACAG TTCTGGAAGC 
ATTGACCATG ATGAATATAA TATCATGAAA GACTTTATGG TTGACTTGGT GAAAAAAGCT 
GATGTTGGCA AGAATCAAGT CCGATTTGGG GCTCTGAAGT ATGCAGATGA CCCGGAGGTG 
CTGTTTTATC TGGATGACCT TAGCACAAAA TGGGAGGTGA TTTCGGTGCT TCAGAAGGAC 
CAGCCCATGG GGGGCAATAC TTACACTGCT GAGGCATTAG GCTTCTCAGA CCACATGTTC 
ACCGAAGCCC GTGGCAGCCG TCTGCACAAA GGGGTCCCCC AAGTCCTCAT TGTGATCACT 
GATGGGGAAT CCCACGATGC GGATAAGCTC AATGACACAG CCAAGGCCTT GAGGGACAAA 
GGCATCCTTG TCCTGGCTGT GGGGATCGCC GGTGCAAATC CTGTGGAGCT GCTAGCTATG 
GCAGGATCGA GTGATAAGTA CTTCTTCGTG GAGACGTTTG GAGGCCTGAA GGGAATATTT 
TCAGATGTGT CAGCCAGTGT ATGTAACTCT TCAAAAGTAG ATTGTGAAAT TGAAAAAGTA 
GATCTTGTCT TCCTCATGGA TGGTTCAAAC AGCATTCATC CAGATGACTT CAAGAAGATG 
AAGGAATTCT TGGCATCTGT TGTTCAAGAC TTTGATGTCA GTGTCAACAG AGTGCGTATA 
GGTGCTGCCC AGTTTAGCCA TACCTATCGG CCAGAATTTC CACTAGGAAC TTTCATAGGC 
AAAAAGGAGA TCTCATTTCA GATTGAAAAC ATCCAGCAAA TCTTTGGATA CACACACATC 
GGTGCCGCAC TCCGAGAGGT GGGGGATTAC TTCCGGCCAG ACATGGGCAG CCGGATAAAC 
GCAGGTACCC CACAGGTATT GCTGGTCCTC ACAGATGGCC AGTCTCAAGA CGAGGTGGCT 
CAGGCTGCTG AAGAGCTGAG ACACAAAGGT ATTGACATTT ATTCTGTGGG CATTGGGGAT 
GTGGATGACC AGCAGCTCAT TCAGATCACC GGGACTGCAG ATAAAAAACT GACGGTTCAT 
AACTTTGATG AACTGACAAA GGTCAAGAAA AGGATAGTTC GCAACATCTG TACCTCGGGG 
GGTGAGAGCA ATTGTTTTGT CGATGTTGTG GTGGGATTTG ACATGTCAAC TCATGAGAAT 
GGGCAAACTT TGCTCGAAGG TCAATCTTGG ATAGAAACCT ACCTTCAAGA CCTTTTACGT 
GCCATCAGCT CCCTCAATGG GGTAAGCTGT GAGGTGGGCA CAGAGACTCA AGTTAGCGTG 
GCTTTTCAAG TGACGAATGC TGTGGAAAAA TATTCCCCCA AGTTTGAGAT CTATAGTGAA 
AACATACTGA ACAGCTTGAA GGATATAACT GTTAAAGGAC CATCTCTACT CAATGCAAAT 
CTCTTGAGTT CTCTGTGGGA TGCATTTCAG AATAAATCAG CTGCTCGAGG AAAGGTGGTT 
CTCTTATTTT CAGATGGATT GGATGATGAC ACTGAAAAGC TTGAACAAAA ATCTGATGAA 
CTTAGAAAAG AAGGCCTGAA TGCCCTCATA ACTGTTGCCC TGGATGGAGC CACTGATTCA 
AGTGACCTGG CTGATCTTCT CTACATTGAA TTCGGGAAAG GGTTTGAGTA CAGGACGCAG 
CTCACTATTG GAATGAGAGA CCTTGGGAGC CGACTGTCAA AGCTACTGGT TAATGTTGCT 
GAAAGGACAT GCTGCTGTTT GTTCTGCAAA TGCATTGGAG GGGATGGCAC AATGCGAGAT 
CCTGGACCAC CGGGGAAAAA GGGATCCCCA GGTTTTAAAG GCAGTGAGGG CTATCTGGGA 
GAGGAAGGTG TTGCTGGAGA CAGAGGAGCC AGTGGACCAA TGGGAGAGCA AGGTACTAAG 
GGATGCTATG GCGCCAAAGG TCCTAAGGGA AACAGGGGAC TAAATGGACA GGAGGGAGAA 
GTTGGAGAAA GCGGAATTGG CGGATTAAAT GGAGAACCGG GTGACAGTGG TCTTCCTGGA 
GAAAAAGGAG AAAAGGGTAA TGAAGGATCC CAGGGAAGCC CAGGAAAGCG AGGAGTTTCT 
GGTGACCGAG GCTCAAAGGG GCAGCGAGGA GACCCCGGAG TTCCTGGATT TGACAATACC 
ATAGAAGGAC CTAAGGGCTT GAAAGGAGAA CGTGGAAGAC AAGGTAGAAG AGGCTGGCCA 
GGCCCCCCTG GAACACCAGG CTCCAGAAGA AAGACAGCAG CTCATGGCCG AAGGGGACAT 
ACAGGCCCAC AGGGGAAAAC AGGCATCCCA GGCCCAGATG GACTTGAAGG CTCATGGGGA 
GTTAAGGGCC CTCAGGGTCC CAGAGGAGAG ACTGGTGTGA AAGGCGAAAA AGGAGGTCTG 
GGAAGTAAAG GTCCTCAGGG GCCTCCAGGA CAAGGAGGAG AAGCAGGGAG TCCAGGCCAT 
CTGGGAAGCC AAGGAACTAA AGGAGAACCT GGAGATTTGG GAGAAAAAGG AGCTATTGGC 
CTCCCGGGTC CTCGAGGCTT ACCGGGTGAT GATGGCAACC CAGGTTATGG TAGCATTGGA 
AGGAAAGGAG CAAAGGGGCA AGATGGATTT CCTGGAGAAA GTGGTCCTAA GGGTGAGATG 
GGGGACCTTG GTGGTCCAGG AGAAACTGGA CCCAAGGGAG CTAGAGGCAA GAGGATATCC 
ACTGGGCTTC CGGGAGAGAT GGGATCCCCT GGGGAGCCAG GACCTCCTGG ACGCAAGGGT 
GTGAAAGGGG CCAGAGGATT GGCTTCATTT TCTATGTGTG AGCTCATTCA GTATGTACGG 
GATCACAGCC CTGGCGGACA TGGAAAACCA GAATGCCCCG TGCATCCCAC CGAATTGGTG 
TTTGCCTTGG ACCAGTCTCG GGATGTCACC AAGCAGGAAT TCGAGCGCAT GAAGGAGCTG 
ATGTCTTCCC TGGTGAGAGG CGTCCGGGTC CGGGAGAACA GCTGCCCGGT GGGCGCCCGC 
ATCGCCATCC TCACCTACAC CTCTCACGCC CGGCACCTCG TCCGCTTCTC AGACGCCTAC 
AAGAAGCAGC AGCTCCTCCG GGAGATTGAA GCTATCCCTT ACGAGAGGTC CTCGGACAGC 
AGGGAGATCG GCGGGGTGAT GAGGTTTATC TCCAGGAATG TCTTCAAGCG AACGCTTCCA 
GGCTCTCACA CAAGAAGAAT CGCCACCTTC TTCAGCAGCG GTCAGTCTGC AGATGCCCAG 
ACCATTGCCA CGGCAGCCAT GGAATTCAGT GCCCTTGACA TCGTTCCGGT GGTCATCGCG 
TTCAGCAACG TGCCCTCCAT CAAGCGTGCA TTTGCAATTG ATGACACTGG CACATTCCAA 
GTAATAATGG TTCCATCCGG GACTGACTAC GCGCCAGCAT TAGAGAGACT ACAGCGGTGT 
ACTTTCTGCT ATGATGTATG CAAGCCAGAC ACTTTTTGCG ATGAAGCCAA ACCACCCCCT 
GCACAGTCTT ACATGGATGC TGCTTTCCTT CTGGACAGCT CTCGGCATGT GCAAAGTGCT 
GAATTTGAAG ACATGAGACA CTTTTTGGGA GCACTCTTAG ATCACTTTGA GATTAGCCCA 
GAGCCTGAGA CCTCTGTCAC TGGAGACCGG GTGGCCCTAT TAAGCCATGC TCCCCCCAAT 
TTCCTACCCA ACACTCAGAA GAGTCCTGTT AAAACTGAGT TCAACCTTAC CACCTACAAT 
AGTAAACGCC TCATGAAGAG GCACATGGAA CAATCAGTTC AACAACTAAA TGGAGATGCT 
TTTATTGGTC ATGCCTTACA GTGGACCCTG GACAATATCT TTTTAAATAC ACCCAACATG 
AGAAGAAACA AAGTCATATT TGTCATATCT GCTGGGGAAA CCAATCACTT GGACAGGGAA 
ACCTTAAAGA AAGAATCCTT AAGAGCCAAA TGTCAAGGTT ATGCCCTATT TGTGTTTTCC 
CTTGGCCCTG CTTGGAATGA CAAAGAACTA GAAGATCTAG CCAGCTACCC TTTGGATCAC 
CACTTGGTCC AGCTTGGCCG AATTCATAAA CCTGATCACA GATATAGTGT GAATTTTGTG 
AAATCCTTTG TAAACTCAAT CAGGCGTGCA ATCAACAAAT ATCCACCAAT AAGCTTCAAA 
ATGAAGTGCA ATAGACTCAC CTCTACAGAT GTGAAGCAGC CTCCACGGCA GTGGAGAAGC 
TTTGTTCCTG GACCACATAA AACTGCCCTC AAAGAAGATG CATTACAGAA GGCAAAGTTC 
TTTCAAGATA AAAAATATCT TTCAAGAGTA GCAAGAGGCA GCAGAGATAT TATTCGAAAT 
CTTACCAGAA ACACATTCCA TACCTTTAAA AATGGAAAAA AGGTGATAAA AACTGCTCCA 
AAACAACATG ATAAAGCAAG TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL6A6 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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