CNAG_00848 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

Gene
Clones
gRNAs

Summary

Gene Symbol	CNAG_00848
Entrez Gene ID	23884624
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Cryptococcus neoformans var. grubii H99

mRNA and Protein(s)

mRNA	Protein	Name
XM_012191611.1	XP_012047001.1	hypothetical protein, variant 1
XM_012191060.1	XP_012046450.1	hypothetical protein
XM_012191612.1	XP_012047002.1	hypothetical protein, variant 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
Janbon G, Ormerod KL, Paulet D, Byrnes EJ 3rd, Yadav V, Chatterjee G, Mullapudi N, Hon CC, Billmyre RB, Brunel F, Bahn YS, Chen W, Chen Y, Chow EW, Copp?e JY, Floyd-Averette A, Gaillardin C, Gerik KJ, Goldberg J, Gonzalez-Hilarion S, Gujja S, Hamlin JL, Hsueh YP, Ianiri G, Jones S, Kodira CD, Kozubowski L, Lam W, Marra M, Mesner LD, Mieczkowski PA, Moyrand F, Nielsen K, Proux C, Rossignol T, Schein JE, Sun S, Wollschlaeger C, Wood IA, Zeng Q, Neuv?glise C, Newlon CS, Perfect JR, Lodge JK, Idnurm A, Stajich JE, Kronstad JW, Sanyal K, Heitman J, Fraser JA, Cuomo CA, Dietrich FS
PLoS genetics10(4)e1004261(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CNAG_00848 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNAG_00848 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCr133694	XM_012191060.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_00848), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCr133694	XM_012191612.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 2 (CNAG_00848), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCr133694	XM_012191611.1 Latest version!	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 1 (CNAG_00848), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNAG_00848 ( XM_012191611.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012047001.1 Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 1 (CNAG_00848), mRNA.

Price & Availability↑

CloneID	OCr133694
Clone ID Related Accession (Same CDS sequence)	XM_012191611.1 , XM_012191060.1 , XM_012191612.1
Accession Version	XM_012191611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026745).

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012047001.1
CDS	411..3329
Translation	MSSLGQKKQAPTPSKTPKKPTQNPEKGASGALGSVKDTAGGATKTAGDTVSGATKQAPGPAKDVGKKVGDTAKDVGGKTSQAAQNPTTPKKAPQPKQAADSPTKKASEGTDKLQKQGEDVAKTAQKQDVKPASAGKGPEKEAAPQTPQEEAAPQTPQEGAAPQTPQEEAAPQTPQQESDDEDKGENQDDNKGEEEEGNDEPVEDEPEGQDQNEGAPEGESGDEDAAKPETLDEPAGDAGGEEQGAPVSDDKEGGVDQAVDKANENKPDDKEGGVDQAVDEANENKPDDKEGGVDQAVDKANENKPEFTGPLTVDQTGKIKDADDRTIASVSEEDAKKLEGSEIADIDASGNLMNKEGDVIGNAELGQGLAQAEEQIDYSVLKGLSPNKAGNLVAKDGITVGKVVDGNLKKLQGRRCTAKGEFFDDAGKKIGQAEPIPEDERSNPEGAPFEDFEGATVQQNGDVVHNGAKVGEIVEGDAKKIAGKTVDADGDVVDKSGTIVGKAQRWYEPEEQEPEKADLSLLAGNRVNKSGYIVDNSGKLLGRVVEGDPAKLAGKMCDKEGQIWNEGGSVVGRAELLPESEREGQKEGPFSDFQPATVRKDGKVIDNSGTVIGRLVEGDAAKLHGKSVDEQGDVVDKNGNKIGSAERWEEEEKEEEKHPAAGLKVNNEGAVIDNNGNTVAKLTEGEISRCRGKEIDNDGDVVDGKGKSIGRVTLVQDLPKRDEAAEQKKAEEEEARKAAEQKKAEEDEERKAAEQKKAEEDEERKKKEDEELKKLEGDSKLAKRMAYEVDSTLDRIKPVLEEMTDLIEDAHSKPKEEVDQEALVKKVKPLIHKSSDILNQCNSNIREMDPDGRMQQQAKENAATGEATPEERHLAEGLKKLSTEVTDAIRKGKKGIEGMPEAKKGISPLWRLLEQPLVQILAAVGLLLSGVLGLVNQLLGGLGLNGLLDGLLGGLGLDKLLGGLGIPKLGGK

Target ORF information:

RefSeq Version	XM_012191611.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 1 (CNAG_00848), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012191611.1

ORF Insert Sequence:

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNAG_00848 ( XM_012191060.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012046450.1 Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_00848), mRNA.

Price & Availability↑

CloneID	OCr133694
Clone ID Related Accession (Same CDS sequence)	XM_012191611.1 , XM_012191060.1 , XM_012191612.1
Accession Version	XM_012191060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026745).

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012046450.1
CDS	414..3332
Translation	MSSLGQKKQAPTPSKTPKKPTQNPEKGASGALGSVKDTAGGATKTAGDTVSGATKQAPGPAKDVGKKVGDTAKDVGGKTSQAAQNPTTPKKAPQPKQAADSPTKKASEGTDKLQKQGEDVAKTAQKQDVKPASAGKGPEKEAAPQTPQEEAAPQTPQEGAAPQTPQEEAAPQTPQQESDDEDKGENQDDNKGEEEEGNDEPVEDEPEGQDQNEGAPEGESGDEDAAKPETLDEPAGDAGGEEQGAPVSDDKEGGVDQAVDKANENKPDDKEGGVDQAVDEANENKPDDKEGGVDQAVDKANENKPEFTGPLTVDQTGKIKDADDRTIASVSEEDAKKLEGSEIADIDASGNLMNKEGDVIGNAELGQGLAQAEEQIDYSVLKGLSPNKAGNLVAKDGITVGKVVDGNLKKLQGRRCTAKGEFFDDAGKKIGQAEPIPEDERSNPEGAPFEDFEGATVQQNGDVVHNGAKVGEIVEGDAKKIAGKTVDADGDVVDKSGTIVGKAQRWYEPEEQEPEKADLSLLAGNRVNKSGYIVDNSGKLLGRVVEGDPAKLAGKMCDKEGQIWNEGGSVVGRAELLPESEREGQKEGPFSDFQPATVRKDGKVIDNSGTVIGRLVEGDAAKLHGKSVDEQGDVVDKNGNKIGSAERWEEEEKEEEKHPAAGLKVNNEGAVIDNNGNTVAKLTEGEISRCRGKEIDNDGDVVDGKGKSIGRVTLVQDLPKRDEAAEQKKAEEEEARKAAEQKKAEEDEERKAAEQKKAEEDEERKKKEDEELKKLEGDSKLAKRMAYEVDSTLDRIKPVLEEMTDLIEDAHSKPKEEVDQEALVKKVKPLIHKSSDILNQCNSNIREMDPDGRMQQQAKENAATGEATPEERHLAEGLKKLSTEVTDAIRKGKKGIEGMPEAKKGISPLWRLLEQPLVQILAAVGLLLSGVLGLVNQLLGGLGLNGLLDGLLGGLGLDKLLGGLGIPKLGGK

Target ORF information:

RefSeq Version	XM_012191060.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_00848), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012191060.1

ORF Insert Sequence:

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CNAG_00848 ( XM_012191612.1 ) cDNA ORF clone, Cryptococcus neoformans var. grubii H99 -> XP_012047002.1 Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 2 (CNAG_00848), mRNA.

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CloneID	OCr133694
Clone ID Related Accession (Same CDS sequence)	XM_012191611.1 , XM_012191060.1 , XM_012191612.1
Accession Version	XM_012191612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Cryptococcus neoformans var. grubii H99
Product	hypothetical protein, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026745).

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012047002.1
CDS	409..3327
Translation	MSSLGQKKQAPTPSKTPKKPTQNPEKGASGALGSVKDTAGGATKTAGDTVSGATKQAPGPAKDVGKKVGDTAKDVGGKTSQAAQNPTTPKKAPQPKQAADSPTKKASEGTDKLQKQGEDVAKTAQKQDVKPASAGKGPEKEAAPQTPQEEAAPQTPQEGAAPQTPQEEAAPQTPQQESDDEDKGENQDDNKGEEEEGNDEPVEDEPEGQDQNEGAPEGESGDEDAAKPETLDEPAGDAGGEEQGAPVSDDKEGGVDQAVDKANENKPDDKEGGVDQAVDEANENKPDDKEGGVDQAVDKANENKPEFTGPLTVDQTGKIKDADDRTIASVSEEDAKKLEGSEIADIDASGNLMNKEGDVIGNAELGQGLAQAEEQIDYSVLKGLSPNKAGNLVAKDGITVGKVVDGNLKKLQGRRCTAKGEFFDDAGKKIGQAEPIPEDERSNPEGAPFEDFEGATVQQNGDVVHNGAKVGEIVEGDAKKIAGKTVDADGDVVDKSGTIVGKAQRWYEPEEQEPEKADLSLLAGNRVNKSGYIVDNSGKLLGRVVEGDPAKLAGKMCDKEGQIWNEGGSVVGRAELLPESEREGQKEGPFSDFQPATVRKDGKVIDNSGTVIGRLVEGDAAKLHGKSVDEQGDVVDKNGNKIGSAERWEEEEKEEEKHPAAGLKVNNEGAVIDNNGNTVAKLTEGEISRCRGKEIDNDGDVVDGKGKSIGRVTLVQDLPKRDEAAEQKKAEEEEARKAAEQKKAEEDEERKAAEQKKAEEDEERKKKEDEELKKLEGDSKLAKRMAYEVDSTLDRIKPVLEEMTDLIEDAHSKPKEEVDQEALVKKVKPLIHKSSDILNQCNSNIREMDPDGRMQQQAKENAATGEATPEERHLAEGLKKLSTEVTDAIRKGKKGIEGMPEAKKGISPLWRLLEQPLVQILAAVGLLLSGVLGLVNQLLGGLGLNGLLDGLLGGLGLDKLLGGLGIPKLGGK

Target ORF information:

RefSeq Version	XM_012191612.1
Organism	Cryptococcus neoformans var. grubii H99
Definition	Cryptococcus neoformans var. grubii H99 hypothetical protein, variant 2 (CNAG_00848), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012191612.1

ORF Insert Sequence:

ATGTCATCGT TAGGACAAAA AAAGCAAGCC CCAACTCCAA GCAAGACCCC CAAGAAGCCT 
ACCCAAAACC CCGAAAAAGG TGCCAGTGGT GCTCTCGGCT CAGTGAAGGA CACCGCTGGC 
GGTGCGACAA AGACTGCAGG AGACACCGTT AGCGGAGCCA CAAAACAGGC GCCTGGTCCC 
GCCAAGGACG TCGGCAAGAA AGTTGGTGAC ACCGCCAAAG ACGTCGGTGG CAAGACCTCC 
CAAGCTGCTC AGAATCCGAC CACGCCGAAG AAGGCGCCGC AACCCAAGCA GGCCGCAGAT 
AGTCCCACAA AGAAGGCCAG CGAGGGCACT GATAAACTCC AAAAGCAAGG CGAAGACGTT 
GCAAAGACAG CGCAGAAGCA AGACGTCAAG CCTGCAAGCG CAGGCAAAGG TCCAGAAAAG 
GAAGCAGCCC CTCAGACTCC TCAGGAGGAA GCAGCCCCTC AGACTCCACA GGAGGGAGCA 
GCCCCTCAGA CCCCCCAGGA GGAAGCAGCC CCTCAGACCC CTCAGCAAGA ATCTGACGAC 
GAAGACAAAG GAGAAAACCA AGACGACAAC AAAGGCGAGG AGGAGGAAGG CAATGACGAG 
CCAGTAGAAG ATGAGCCCGA GGGGCAGGAT CAAAACGAGG GCGCTCCTGA AGGAGAGTCA 
GGCGATGAGG ACGCAGCTAA GCCTGAGACT CTGGACGAAC CTGCGGGCGA TGCCGGCGGT 
GAAGAGCAAG GCGCACCCGT TTCCGACGAT AAGGAAGGCG GCGTCGACCA AGCAGTCGAC 
AAAGCCAATG AAAACAAGCC CGACGACAAG GAAGGCGGCG TCGACCAAGC AGTAGACGAA 
GCCAATGAAA ACAAGCCCGA CGACAAGGAA GGCGGCGTCG ACCAAGCAGT AGACAAAGCC 
AATGAAAACA AGCCCGAGTT CACAGGCCCT CTGACAGTTG ACCAAACTGG CAAGATCAAA 
GACGCCGATG ACAGGACTAT TGCAAGTGTA TCTGAGGAGG ACGCCAAAAA GCTTGAAGGC 
TCAGAGATCG CCGACATTGA TGCTTCTGGC AACCTTATGA ACAAAGAGGG CGACGTGATC 
GGTAACGCAG AGCTTGGGCA AGGGCTTGCC CAGGCCGAAG AGCAGATTGA CTACAGCGTG 
CTCAAGGGTC TCAGTCCCAA CAAAGCCGGC AATTTGGTCG CAAAGGATGG CATCACGGTT 
GGCAAAGTCG TCGACGGCAA CCTCAAGAAG CTCCAAGGTC GACGATGCAC CGCAAAGGGC 
GAATTCTTTG ACGATGCAGG CAAAAAGATT GGACAAGCTG AGCCAATTCC AGAAGACGAG 
AGGTCGAATC CAGAAGGTGC TCCTTTCGAA GACTTTGAGG GCGCTACAGT CCAGCAGAAT 
GGTGATGTAG TGCACAATGG AGCGAAGGTT GGAGAGATTG TCGAAGGAGA CGCAAAGAAG 
ATTGCAGGAA AGACAGTCGA TGCCGATGGT GACGTCGTTG ACAAGTCCGG TACGATCGTC 
GGCAAAGCAC AGCGATGGTA CGAGCCAGAA GAGCAGGAGC CAGAGAAGGC CGACCTCTCT 
CTGCTCGCCG GCAATCGTGT TAACAAGAGT GGTTACATCG TCGATAATTC TGGCAAGCTA 
CTCGGACGAG TGGTTGAGGG CGATCCTGCC AAGCTCGCTG GCAAGATGTG CGACAAAGAG 
GGGCAGATTT GGAATGAAGG TGGTAGCGTC GTAGGACGCG CCGAGCTGTT GCCCGAGTCT 
GAGCGCGAAG GACAGAAAGA AGGCCCCTTC TCTGATTTCC AGCCGGCAAC CGTTCGCAAA 
GACGGCAAGG TCATTGACAA TTCGGGAACG GTTATTGGAC GTCTGGTTGA GGGCGATGCC 
GCGAAGCTTC ACGGCAAGTC AGTAGATGAG CAAGGCGACG TTGTCGACAA GAACGGTAAT 
AAGATCGGTT CCGCCGAACG ATGGGAAGAA GAGGAGAAAG AAGAAGAGAA GCATCCTGCT 
GCAGGTCTCA AGGTTAACAA CGAAGGGGCT GTCATCGATA ACAATGGAAA CACCGTGGCC 
AAGCTTACAG AGGGCGAGAT CTCACGTTGC CGAGGCAAAG AAATTGACAA CGACGGAGAC 
GTCGTAGATG GGAAAGGAAA GAGTATCGGC CGCGTAACGC TCGTGCAGGA TCTGCCTAAG 
AGAGATGAGG CGGCCGAGCA GAAGAAAGCA GAAGAAGAGG AAGCACGTAA GGCAGCCGAG 
CAGAAGAAAG CAGAGGAAGA TGAGGAACGA AAGGCAGCCG AACAGAAGAA AGCAGAGGAA 
GACGAAGAAC GCAAGAAGAA AGAGGATGAG GAGCTCAAGA AGCTCGAGGG CGACAGCAAG 
CTGGCCAAAC GCATGGCTTA CGAAGTTGAC AGTACACTTG ATCGTATCAA GCCTGTACTC 
GAAGAAATGA CGGACCTGAT CGAGGATGCT CACTCCAAGC CCAAAGAGGA AGTCGACCAA 
GAAGCACTTG TGAAGAAAGT CAAGCCGCTT ATTCACAAGA GCTCCGACAT CCTCAACCAG 
TGCAACTCCA ACATTCGCGA GATGGATCCC GACGGTCGAA TGCAGCAACA GGCAAAGGAG 
AATGCTGCCA CTGGTGAAGC TACTCCAGAA GAGCGACACC TCGCTGAAGG CCTCAAGAAG 
CTTTCGACTG AAGTCACGGA TGCTATCAGG AAGGGCAAGA AGGGTATCGA AGGTATGCCC 
GAAGCTAAGA AGGGGATCAG TCCACTTTGG CGCCTGCTCG AGCAACCATT GGTGCAAATC 
CTTGCAGCAG TCGGGTTACT TTTGTCCGGT GTTCTGGGCC TTGTGAACCA GCTTTTGGGC 
GGGCTTGGTC TGAATGGCCT GCTCGATGGT CTCCTTGGTG GATTGGGTCT TGACAAGCTC 
CTAGGCGGTC TTGGAATTCC TAAACTTGGC GGAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation.
PLoS genetics10(4)e1004261(2014 Apr)
Janbon G,Ormerod KL,Paulet D,Byrnes EJ 3rd,Yadav V,Chatterjee G,Mullapudi N,Hon CC,Billmyre RB,Brunel F,Bahn YS,Chen W,Chen Y,Chow EW,Copp?e JY,Floyd-Averette A,Gaillardin C,Gerik KJ,Goldberg J,Gonzalez-Hilarion S,Gujja S,Hamlin JL,Hsueh YP,Ianiri G,Jones S,Kodira CD,Kozubowski L,Lam W,Marra M,Mesner LD,Mieczkowski PA,Moyrand F,Nielsen K,Proux C,Rossignol T,Schein JE,Sun S,Wollschlaeger C,Wood IA,Zeng Q,Neuv?glise C,Newlon CS,Perfect JR,Lodge JK,Idnurm A,Stajich JE,Kronstad JW,Sanyal K,Heitman J,Fraser JA,Cuomo CA,Dietrich FS

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CNAG_00848 cDNA ORF clone, Cryptococcus neoformans var. grubii H99

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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