chs1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chs1
Entrez Gene ID	322468
Full Name	chitin synthase 1
Synonyms	fb62c02,si:zfos-1762d12.1,wu:fb62c02
General protein information	Preferred Names chitin synthase 1 Names uncharacterized protein LOC322468
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307025.2	XP_009305300.1	uncharacterized protein LOC322468 isoform X1
XM_009307025.3	XP_009305300.1	uncharacterized protein LOC322468 isoform X1
NM_001316326.1	NP_001303255.1	uncharacterized protein LOC322468

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:zfos-1762d12.1	XP_001921014.3
Drosophila melanogaster (fruit fly)	CS-2	NP_524209.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001205	XP_321951.2
Danio rerio (zebrafish)	si:ch211-219a15.4	XP_691594.4
Danio rerio (zebrafish)	si:ch211-264e16.2	XP_691803.4
Danio rerio (zebrafish)	LOC101886093	XP_005170020.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chitin is endogenously produced in vertebrates.
Tang WJ, Fernandez J, Sohn JJ, Amemiya CT
Current biology : CB25(7)897-900(2015 Mar)

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Feng H, Zhang YB, Zhang QM, Li Z, Zhang QY, Gui JF
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18334	NM_001316326.1 Latest version!	Danio rerio chitin synthase 1 (chs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39715	XM_009307025.3 Latest version!	Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa39715	XM_009307025.2	Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chs1 ( NM_001316326.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001303255.1 Danio rerio chitin synthase 1 (chs1), mRNA.

Price & Availability↑

CloneID	ODa18334
Clone ID Related Accession (Same CDS sequence)	NM_001316326.1
Accession Version	NM_001316326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC322468
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU694274.5. On Oct 16, 2015 this sequence version replaced XM_001920979.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01000281.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCA GCTCCAGCAG CTCTGAGAAG 
AAGATGGACA CGTTCAATGA CGACACAGAC TATGAGGACA CAGACTACGA TGATGATGAC 
GACGACGAGG ATTATGATTA TAATACTGTG GAGCCCAAAG AAGTTGTGCC TGAGAGCGCC 
TGGGTGGAGC CCGTGAAGAG GGAGTTCCTG AAGAAGCTGA CCTACGCCAA TCTGAAGAGG 
AACCTTCAGG AGCAGATCCG CTACACGCTG AGGAACAAGA ACCAGGAGGA CCTGTGCGAG 
GAGCTGGTGC TGATCCTGAC CGACACGCTG AACGACGAGC TGAAGGATAA AGTGGGTCCT 
GAAGACGTCT TGACCGAGAC GCAGCTGGAG GAACTGCAGT ATGCGCTCAA TGAAGCGGCG 
CGGCGCATCG TCAAGACCAA CCGCATCGAG ACCGGCTCAC AGCGGGTGGA GCGGCGGGTC 
AAACGGGGCA TCGAGAGGAC GCTGGTGGCG CCGCAGGTGG AGAAACTGTC AGAGGACGAG 
CAGGATTTCT GGGAGAAGCT GTTGGAGCGC TACCTGAAGC CCATTCAGGA TGATAAAGCT 
CATCTGGAGG AGGTGACGCG GGAGCTCAAG TCTCTGAGGA ACAAAGCCGT GTTTCTGTAC 
TTCATCATTA ACGTCCTCTG GGTCGTAGCC ACGTTCTTCC TGCAGGCCAT CGGCGGAGAC 
GTTCTGAGTA TAGAAATCCC CAAATATTAC CCCAATAAAA CCCTGGCCCC GGAGCCCCTG 
AGGGTGGAGC CGCTCAGCCT GATGTTCCTG CTGTCCTTCG CCGTCCTGCT GGTCGTGCAG 
TTCCTGGCTA TGCTGTACCA CAGGGTCTAC ACGCTCATCC ATGTGGTGTC GTACAGAAGC 
TCAGAGAAGA ACTATAAAGA GAAGGATGAG GAGGATGAGG AAGATGCGCT GATCTTGGGG 
AACATCATCC CCAGCAGTCT GGTGATCACC GGAGACGACC TGTTGAACGG AGACTCCTTC 
ATTCCGAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303255.1
CDS	59..4450
Translation	MEELKQRGQKREGRHRDTWDPFQLNPIGAEKEKKQVCFRILQNLVAVLVGLAVLVSGVVSKGSLLVLTTLAGPKSLRDEVEQQFYGLLLMCVLVTPNLLMFLKALWKCVFKSYVAPNPRTLGIVCGIEALVALGTAMLVLIVMPNLDILTNLCIPGGICIFSAVLQIAFRLQTRRWMILYPICSLILVVSGYFLLGIDYYVREKTYIKHFSDRLENVDDYMTYVYVGIAIFASLLVSLNWWENPFQASTNIQEMLSELDTFRDTAYVFTSFIRIVVTLAVYFLYFFVIANNQFEWDAYTKASQATQEIALGLFFLQAFCSAACHWFGVVACKIHAMKFGFALPVSLIGPATLILGMILFVTQSAQFNPSPEIAAIIKGNITNDFWPFCYELKSLSDVNTTPVVMLEISYSICKTSLNNPYEMWPFSMLALEGVCMWLALVSCTYYIWKIKVQRIERTSLLFVRRLYESAFIDISMLLNTKMKVIRTANRESNNDAELENCVIYLCATMWHETYDEMLKILTSMFRLDRYRGDPKEEHKDCFDFECHIYVDDAFMIEKDSGTKFVNTYVEDLIHVVMEVYRVFTNKEPDEVSIIETPYGGRLVFVMPEGNMLYVHLKDKQLIRNKKRWSQIMYLYYLLGWKGYNVKNPQKITRQNNPCRASLVSLDSETYLLPGLDNDSKRRHISDDNTYIMALDGDTDFHPKALILLVDRLRMYDNVGAACGRIHPTGMGPMVWYQKFEYAVGHWLQKTAEHVFGSVLCSPGCFSLFRGSALMDDNVLKRYTTKATRASEYVQYDQGEDRWLCTLLLQQGWRVEYNAASDAYTNSPQEFKEFYNQRRRWGPSTLANTLDLLHSGKETVKRNTSISMLYILYQIFTVASSILGPASVTLMIAGAFQFVFGVSGTLSIIIAVIPPVFYMLICFLAKPNLQITIAAILSVIYAFLMTASFFAIIGDMVIQGTFITPTGLFLVSGAILYMVTAVLHPQECTLIIYGLMYFICIPSGYLLLTIYSLVNMHIVSWGTRESTKGKEQKKQVGVVCNRDCRMCCWDVKIQVTQETENLLLQQMQQAVNPTAAAAAKAEPEEPATLSAAPEHSSVEISRHVQEKNMENKKDQSKENLNKDNKERDSESERSSSSSSSEKKMDTFNDDTDYEDTDYDDDDDDEDYDYNTVEPKEVVPESAWVEPVKREFLKKLTYANLKRNLQEQIRYTLRNKNQEDLCEELVLILTDTLNDELKDKVGPEDVLTETQLEELQYALNEAARRIVKTNRIETGSQRVERRVKRGIERTLVAPQVEKLSEDEQDFWEKLLERYLKPIQDDKAHLEEVTRELKSLRNKAVFLYFIINVLWVVATFFLQAIGGDVLSIEIPKYYPNKTLAPEPLRVEPLSLMFLLSFAVLLVVQFLAMLYHRVYTLIHVVSYRSSEKNYKEKDEEDEEDALILGNIIPSSLVITGDDLLNGDSFIPS

Target ORF information:

RefSeq Version	NM_001316326.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chitin synthase 1 (chs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316326.1

ORF Insert Sequence:

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCA GCTCCAGCAG CTCTGAGAAG 
AAGATGGACA CGTTCAATGA CGACACAGAC TATGAGGACA CAGACTACGA TGATGATGAC 
GACGACGAGG ATTATGATTA TAATACTGTG GAGCCCAAAG AAGTTGTGCC TGAGAGCGCC 
TGGGTGGAGC CCGTGAAGAG GGAGTTCCTG AAGAAGCTGA CCTACGCCAA TCTGAAGAGG 
AACCTTCAGG AGCAGATCCG CTACACGCTG AGGAACAAGA ACCAGGAGGA CCTGTGCGAG 
GAGCTGGTGC TGATCCTGAC CGACACGCTG AACGACGAGC TGAAGGATAA AGTGGGTCCT 
GAAGACGTCT TGACCGAGAC GCAGCTGGAG GAACTGCAGT ATGCGCTCAA TGAAGCGGCG 
CGGCGCATCG TCAAGACCAA CCGCATCGAG ACCGGCTCAC AGCGGGTGGA GCGGCGGGTC 
AAACGGGGCA TCGAGAGGAC GCTGGTGGCG CCGCAGGTGG AGAAACTGTC AGAGGACGAG 
CAGGATTTCT GGGAGAAGCT GTTGGAGCGC TACCTGAAGC CCATTCAGGA TGATAAAGCT 
CATCTGGAGG AGGTGACGCG GGAGCTCAAG TCTCTGAGGA ACAAAGCCGT GTTTCTGTAC 
TTCATCATTA ACGTCCTCTG GGTCGTAGCC ACGTTCTTCC TGCAGGCCAT CGGCGGAGAC 
GTTCTGAGTA TAGAAATCCC CAAATATTAC CCCAATAAAA CCCTGGCCCC GGAGCCCCTG 
AGGGTGGAGC CGCTCAGCCT GATGTTCCTG CTGTCCTTCG CCGTCCTGCT GGTCGTGCAG 
TTCCTGGCTA TGCTGTACCA CAGGGTCTAC ACGCTCATCC ATGTGGTGTC GTACAGAAGC 
TCAGAGAAGA ACTATAAAGA GAAGGATGAG GAGGATGAGG AAGATGCGCT GATCTTGGGG 
AACATCATCC CCAGCAGTCT GGTGATCACC GGAGACGACC TGTTGAACGG AGACTCCTTC 
ATTCCGAGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs1 ( XM_009307025.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305300.1 Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39715
Clone ID Related Accession (Same CDS sequence)	XM_009307025.2 , XM_009307025.3
Accession Version	XM_009307025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	uncharacterized protein LOC322468 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG 
ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC 
GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG 
GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC 
CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG 
CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA 
GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG 
CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA 
CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG 
GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT 
CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC 
ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT 
CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG 
GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC 
CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA 
GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC 
ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT 
CCGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305300.1
CDS	11..4399
Translation	MEELKQRGQKREGRHRDTWDPFQLNPIGAEKEKKQVCFRILQNLVAVLVGLAVLVSGVVSKGSLLVLTTLAGPKSLRDEVEQQFYGLLLMCVLVTPNLLMFLKALWKCVFKSYVAPNPRTLGIVCGIEALVALGTAMLVLIVMPNLDILTNLCIPGGICIFSAVLQIAFRLQTRRWMILYPICSLILVVSGYFLLGIDYYVREKTYIKHFSDRLENVDDYMTYVYVGIAIFASLLVSLNWWENPFQASTNIQEMLSELDTFRDTAYVFTSFIRIVVTLAVYFLYFFVIANNQFEWDAYTKASQATQEIALGLFFLQAFCSAACHWFGVVACKIHAMKFGFALPVSLIGPATLILGMILFVTQSAQFNPSPEIAAIIKGNITNDFWPFCYELKSLSDVNTTPVVMLEISYSICKTSLNNPYEMWPFSMLALEGVCMWLALVSCTYYIWKIKVQRIERTSLLFVRRLYESAFIDISMLLNTKMKVIRTANRESNNDAELENCVIYLCATMWHETYDEMLKILTSMFRLDRYRGDPKEEHKDCFDFECHIYVDDAFMIEKDSGTKFVNTYVEDLIHVVMEVYRVFTNKEPDEVSIIETPYGGRLVFVMPEGNMLYVHLKDKQLIRNKKRWSQIMYLYYLLGWKGYNVKNPQKITRQNNPCRASLVSLDSETYLLPGLDNDSKRRHISDDNTYIMALDGDTDFHPKALILLVDRLRMYDNVGAACGRIHPTGMGPMVWYQKFEYAVGHWLQKTAEHVFGSVLCSPGCFSLFRGSALMDDNVLKRYTTKATRASEYVQYDQGEDRWLCTLLLQQGWRVEYNAASDAYTNSPQEFKEFYNQRRRWGPSTLANTLDLLHSGKETVKRNTSISMLYILYQIFTVASSILGPASVTLMIAGAFQFVFGVSGTLSIIIAVIPPVFYMLICFLAKPNLQITIAAILSVIYAFLMTASFFAIIGDMVIQGTFITPTGLFLVSGAILYMVTAVLHPQECTLIIYGLMYFICIPSGYLLLTIYSLVNMHIVSWGTRESTKGKEQKKQVGVVCNRDCRMCCWDVKIQVTQETENLLLQQMQQAVNPTAAAAAKAEPEEPATLSAAPEHSSVEISRHVQEKNMENKKDQSKENLNKDNKERDSESERSSSSSSEKKMDTFNDDTDYEDTDYDDDDDDEDYDYNTVEPKEVVPESAWVEPVKREFLKKLTYANLKRNLQEQIRYTLRNKNQEDLCEELVLILTDTLNDELKDKVGPEDVLTETQLEELQYALNEAARRIVKTNRIETGSQRVERRVKRGIERTLVAPQVEKLSEDEQDFWEKLLERYLKPIQDDKAHLEEVTRELKSLRNKAVFLYFIINVLWVVATFFLQAIGGDVLSIEIPKYYPNKTLAPEPLRVEPLSLMFLLSFAVLLVVQFLAMLYHRVYTLIHVVSYRSSEKNYKEKDEEDEEDALILGNIIPSSLVITGDDLLNGDSFIPS

Target ORF information:

RefSeq Version	XM_009307025.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307025.2

ORF Insert Sequence:

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG 
ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC 
GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG 
GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC 
CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG 
CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA 
GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG 
CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA 
CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG 
GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT 
CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC 
ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT 
CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG 
GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC 
CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA 
GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC 
ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT 
CCGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chs1 ( XM_009307025.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305300.1 Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

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CloneID	ODa39715
Clone ID Related Accession (Same CDS sequence)	XM_009307025.2 , XM_009307025.3
Accession Version	XM_009307025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	uncharacterized protein LOC322468 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG 
ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC 
GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG 
GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC 
CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG 
CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA 
GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG 
CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA 
CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG 
GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT 
CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC 
ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT 
CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG 
GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC 
CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA 
GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC 
ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT 
CCGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305300.1
CDS	35..4423
Translation	MEELKQRGQKREGRHRDTWDPFQLNPIGAEKEKKQVCFRILQNLVAVLVGLAVLVSGVVSKGSLLVLTTLAGPKSLRDEVEQQFYGLLLMCVLVTPNLLMFLKALWKCVFKSYVAPNPRTLGIVCGIEALVALGTAMLVLIVMPNLDILTNLCIPGGICIFSAVLQIAFRLQTRRWMILYPICSLILVVSGYFLLGIDYYVREKTYIKHFSDRLENVDDYMTYVYVGIAIFASLLVSLNWWENPFQASTNIQEMLSELDTFRDTAYVFTSFIRIVVTLAVYFLYFFVIANNQFEWDAYTKASQATQEIALGLFFLQAFCSAACHWFGVVACKIHAMKFGFALPVSLIGPATLILGMILFVTQSAQFNPSPEIAAIIKGNITNDFWPFCYELKSLSDVNTTPVVMLEISYSICKTSLNNPYEMWPFSMLALEGVCMWLALVSCTYYIWKIKVQRIERTSLLFVRRLYESAFIDISMLLNTKMKVIRTANRESNNDAELENCVIYLCATMWHETYDEMLKILTSMFRLDRYRGDPKEEHKDCFDFECHIYVDDAFMIEKDSGTKFVNTYVEDLIHVVMEVYRVFTNKEPDEVSIIETPYGGRLVFVMPEGNMLYVHLKDKQLIRNKKRWSQIMYLYYLLGWKGYNVKNPQKITRQNNPCRASLVSLDSETYLLPGLDNDSKRRHISDDNTYIMALDGDTDFHPKALILLVDRLRMYDNVGAACGRIHPTGMGPMVWYQKFEYAVGHWLQKTAEHVFGSVLCSPGCFSLFRGSALMDDNVLKRYTTKATRASEYVQYDQGEDRWLCTLLLQQGWRVEYNAASDAYTNSPQEFKEFYNQRRRWGPSTLANTLDLLHSGKETVKRNTSISMLYILYQIFTVASSILGPASVTLMIAGAFQFVFGVSGTLSIIIAVIPPVFYMLICFLAKPNLQITIAAILSVIYAFLMTASFFAIIGDMVIQGTFITPTGLFLVSGAILYMVTAVLHPQECTLIIYGLMYFICIPSGYLLLTIYSLVNMHIVSWGTRESTKGKEQKKQVGVVCNRDCRMCCWDVKIQVTQETENLLLQQMQQAVNPTAAAAAKAEPEEPATLSAAPEHSSVEISRHVQEKNMENKKDQSKENLNKDNKERDSESERSSSSSSEKKMDTFNDDTDYEDTDYDDDDDDEDYDYNTVEPKEVVPESAWVEPVKREFLKKLTYANLKRNLQEQIRYTLRNKNQEDLCEELVLILTDTLNDELKDKVGPEDVLTETQLEELQYALNEAARRIVKTNRIETGSQRVERRVKRGIERTLVAPQVEKLSEDEQDFWEKLLERYLKPIQDDKAHLEEVTRELKSLRNKAVFLYFIINVLWVVATFFLQAIGGDVLSIEIPKYYPNKTLAPEPLRVEPLSLMFLLSFAVLLVVQFLAMLYHRVYTLIHVVSYRSSEKNYKEKDEEDEEDALILGNIIPSSLVITGDDLLNGDSFIPS

Target ORF information:

RefSeq Version	XM_009307025.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chitin synthase 1 (chs1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307025.3

ORF Insert Sequence:

ATGGAGGAGC TGAAGCAGCG GGGGCAGAAG AGGGAAGGAC GACACAGGGA CACATGGGAC 
CCGTTCCAGC TGAACCCCAT CGGGGCGGAG AAGGAGAAGA AGCAAGTGTG TTTCCGGATC 
CTGCAGAATC TGGTGGCGGT GCTGGTGGGT CTGGCGGTGC TGGTCAGCGG CGTCGTCAGC 
AAGGGCTCTC TGCTGGTTCT GACCACTCTG GCTGGTCCGA AGTCTCTGAG AGATGAAGTG 
GAGCAGCAGT TCTACGGGCT GCTGCTGATG TGTGTTCTGG TGACCCCAAA CCTCCTGATG 
TTCCTCAAGG CCTTGTGGAA GTGTGTCTTC AAGAGTTACG TGGCTCCAAA CCCGAGAACA 
CTGGGAATCG TGTGTGGGAT TGAGGCTCTG GTGGCGCTCG GGACCGCAAT GCTGGTGCTC 
ATTGTGATGC CAAACCTTGA CATCCTCACA AACCTGTGCA TCCCGGGCGG GATCTGCATC 
TTTTCAGCCG TGCTCCAGAT CGCCTTCCGC CTGCAGACAC GCAGATGGAT GATCCTCTAC 
CCCATCTGCT CCCTCATTCT AGTGGTCAGC GGATACTTTC TGCTTGGCAT CGACTACTAC 
GTGAGAGAGA AAACTTATAT CAAACATTTC TCTGATCGCC TTGAGAATGT GGATGATTAT 
ATGACCTACG TGTATGTCGG CATTGCTATA TTTGCGTCTC TGCTGGTTTC CCTGAACTGG 
TGGGAGAACC CTTTTCAGGC CAGCACCAAC ATCCAGGAGA TGCTCAGTGA GCTGGACACA 
TTTAGAGACA CTGCTTATGT GTTCACCAGC TTCATCCGTA TAGTCGTGAC TCTGGCCGTG 
TACTTCCTGT ATTTCTTTGT TATTGCAAAT AATCAATTCG AATGGGACGC TTACACCAAA 
GCCAGCCAAG CCACGCAGGA GATCGCCCTG GGCCTGTTTT TCCTGCAGGC CTTCTGCTCG 
GCTGCGTGCC ACTGGTTTGG AGTCGTGGCC TGTAAGATCC ACGCAATGAA ATTCGGCTTT 
GCTCTGCCGG TGTCATTGAT CGGTCCAGCA ACTCTGATTC TGGGCATGAT CCTGTTCGTG 
ACTCAGTCTG CACAGTTCAA CCCTTCACCT GAAATCGCCG CCATAATCAA AGGAAACATC 
ACCAATGACT TCTGGCCATT TTGCTACGAA CTTAAATCTC TCAGCGATGT CAACACCACG 
CCGGTTGTGA TGCTGGAGAT CTCCTACAGC ATCTGCAAGA CTTCGCTCAA TAATCCGTAC 
GAGATGTGGC CGTTCTCGAT GCTGGCGCTG GAGGGGGTGT GCATGTGGCT GGCGCTCGTC 
TCCTGCACGT ATTACATCTG GAAAATCAAG GTGCAGAGGA TCGAGCGCAC ATCGCTGCTG 
TTCGTACGCC GCCTGTACGA GTCCGCCTTC ATTGACATCT CAATGCTACT CAACACCAAA 
ATGAAGGTCA TTCGCACGGC GAACCGGGAG AGTAATAACG ATGCTGAACT GGAAAACTGT 
GTCATTTACC TTTGTGCAAC GATGTGGCAT GAAACTTATG ATGAGATGCT GAAAATCTTG 
ACTTCAATGT TCAGATTGGA CAGATATCGA GGGGATCCAA AGGAGGAACA CAAGGATTGC 
TTTGACTTTG AGTGCCATAT CTACGTTGAC GACGCCTTCA TGATAGAAAA GGACTCTGGG 
ACAAAGTTTG TCAACACCTA TGTTGAAGAC CTCATACATG TTGTGATGGA GGTGTACAGG 
GTGTTCACCA ATAAGGAGCC CGATGAGGTG TCTATCATTG AGACGCCATA CGGAGGTCGA 
CTTGTGTTCG TCATGCCCGA GGGAAACATG CTTTACGTCC ACCTGAAAGA CAAACAGCTG 
ATTCGAAACA AGAAGAGATG GTCACAGATC ATGTACCTGT ATTACCTGCT CGGCTGGAAA 
GGATACAACG TCAAAAACCC TCAGAAAATA ACCAGACAAA ACAATCCCTG CCGTGCCAGT 
CTTGTTTCGC TGGATAGTGA GACGTATTTA CTGCCCGGTC TGGATAATGA CAGTAAAAGG 
AGGCATATAT CTGACGACAA CACATACATC ATGGCTCTGG ACGGAGACAC AGACTTCCAC 
CCGAAGGCTC TGATTCTGCT GGTGGACCGA TTGAGGATGT ACGACAACGT GGGCGCAGCT 
TGCGGACGCA TTCATCCAAC TGGAATGGGA CCGATGGTGT GGTATCAGAA GTTTGAGTAT 
GCGGTGGGCC ACTGGCTCCA GAAAACAGCA GAGCACGTGT TCGGTTCGGT TCTCTGCAGT 
CCCGGATGCT TCAGTCTGTT CAGAGGATCT GCACTGATGG ACGACAACGT ACTGAAGAGA 
TACACAACTA AAGCTACTCG CGCATCTGAG TATGTGCAGT ACGATCAAGG GGAGGACCGC 
TGGCTGTGCA CTCTCCTCCT GCAGCAGGGC TGGCGGGTGG AGTATAACGC CGCCTCGGAC 
GCCTACACCA ACTCTCCGCA GGAGTTCAAG GAGTTTTACA ACCAGAGGCG GCGCTGGGGT 
CCGTCAACCC TGGCCAACAC CCTGGACCTG CTGCACAGCG GGAAGGAGAC GGTCAAGAGG 
AACACCTCCA TATCCATGCT CTACATCCTC TATCAGATCT TCACCGTGGC CTCCTCCATC 
CTGGGACCCG CGTCTGTCAC GCTCATGATC GCAGGAGCGT TCCAGTTTGT GTTTGGCGTG 
TCCGGCACTC TCTCCATCAT AATCGCTGTC ATCCCTCCAG TGTTTTACAT GCTCATCTGT 
TTCCTGGCCA AACCCAACCT ACAAATCACC ATCGCCGCCA TTCTCAGCGT CATTTACGCC 
TTCCTGATGA CGGCATCCTT CTTCGCCATC ATCGGTGACA TGGTGATCCA GGGCACATTC 
ATAACCCCCA CCGGCCTGTT CCTGGTGTCC GGCGCTATCC TGTACATGGT GACGGCGGTG 
CTCCACCCGC AGGAGTGCAC GCTGATCATC TACGGCCTGA TGTACTTCAT CTGCATCCCC 
AGCGGGTATC TCCTGCTCAC CATATACTCG CTGGTCAACA TGCACATCGT GTCCTGGGGC 
ACGCGGGAGT CCACCAAAGG CAAGGAGCAG AAAAAGCAGG TGGGTGTGGT GTGCAACCGA 
GACTGCAGGA TGTGCTGCTG GGATGTCAAG ATCCAGGTCA CGCAGGAGAC CGAGAACCTC 
CTCCTTCAGC AAATGCAGCA GGCCGTCAAT CCCACCGCCG CCGCCGCAGC TAAAGCGGAG 
CCGGAAGAGC CGGCGACTCT GAGCGCAGCT CCAGAACACA GCAGTGTGGA GATCAGCAGA 
CACGTCCAGG AGAAAAACAT GGAGAACAAG AAGGACCAGA GCAAGGAGAA CCTCAACAAG 
GACAACAAGG AGAGAGACTC AGAGTCTGAG AGAAGCAGCT CCAGCAGCTC TGAGAAGAAG 
ATGGACACGT TCAATGACGA CACAGACTAT GAGGACACAG ACTACGATGA TGATGACGAC 
GACGAGGATT ATGATTATAA TACTGTGGAG CCCAAAGAAG TTGTGCCTGA GAGCGCCTGG 
GTGGAGCCCG TGAAGAGGGA GTTCCTGAAG AAGCTGACCT ACGCCAATCT GAAGAGGAAC 
CTTCAGGAGC AGATCCGCTA CACGCTGAGG AACAAGAACC AGGAGGACCT GTGCGAGGAG 
CTGGTGCTGA TCCTGACCGA CACGCTGAAC GACGAGCTGA AGGATAAAGT GGGTCCTGAA 
GACGTCTTGA CCGAGACGCA GCTGGAGGAA CTGCAGTATG CGCTCAATGA AGCGGCGCGG 
CGCATCGTCA AGACCAACCG CATCGAGACC GGCTCACAGC GGGTGGAGCG GCGGGTCAAA 
CGGGGCATCG AGAGGACGCT GGTGGCGCCG CAGGTGGAGA AACTGTCAGA GGACGAGCAG 
GATTTCTGGG AGAAGCTGTT GGAGCGCTAC CTGAAGCCCA TTCAGGATGA TAAAGCTCAT 
CTGGAGGAGG TGACGCGGGA GCTCAAGTCT CTGAGGAACA AAGCCGTGTT TCTGTACTTC 
ATCATTAACG TCCTCTGGGT CGTAGCCACG TTCTTCCTGC AGGCCATCGG CGGAGACGTT 
CTGAGTATAG AAATCCCCAA ATATTACCCC AATAAAACCC TGGCCCCGGA GCCCCTGAGG 
GTGGAGCCGC TCAGCCTGAT GTTCCTGCTG TCCTTCGCCG TCCTGCTGGT CGTGCAGTTC 
CTGGCTATGC TGTACCACAG GGTCTACACG CTCATCCATG TGGTGTCGTA CAGAAGCTCA 
GAGAAGAACT ATAAAGAGAA GGATGAGGAG GATGAGGAAG ATGCGCTGAT CTTGGGGAAC 
ATCATCCCCA GCAGTCTGGT GATCACCGGA GACGACCTGT TGAACGGAGA CTCCTTCATT 
CCGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chitin is endogenously produced in vertebrates.
Current biology : CB25(7)897-900(2015 Mar)
Tang WJ,Fernandez J,Sohn JJ,Amemiya CT

Zebrafish IRF1 regulates IFN antiviral response through binding to IFN?1 and IFN?3 promoters downstream of MyD88 signaling.
Journal of immunology (Baltimore, Md. : 1950)194(3)1225-38(2015 Feb)
Feng H,Zhang YB,Zhang QM,Li Z,Zhang QY,Gui JF

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chs1 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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