mbd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mbd2
Entrez Gene ID	337105
Full Name	methyl-CpG binding domain protein 2
Synonyms	wu:fa97f06,zgc:111862
General protein information	Preferred Names methyl-CpG binding domain protein 2 Names methyl-CpG-binding domain protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212768.1	NP_997933.1	methyl-CpG-binding domain protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MBD2	XP_512139.4
Danio rerio (zebrafish)	mbd2	NP_997933.1
Homo sapiens (human)	MBD2	NP_003918.1
Bos taurus (cattle)	MBD2	XP_002697814.1
Rattus norvegicus (Norway rat)	Mbd2	NP_001108497.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003982	XP_003436675.1
	MBD2	XP_001082249.1
Mus musculus (house mouse)	Mbd2	NP_034903.2
Gallus gallus (chicken)	MBD2	NP_001012403.1
Canis lupus familiaris (dog)	MBD2	XP_005615735.1
Drosophila melanogaster (fruit fly)	MBD-like	NP_731370.1

Gene Ontology
Bibliography

Related articles in PubMed

Bisphenol A causes reproductive toxicity, decreases dnmt1 transcription, and reduces global DNA methylation in breeding zebrafish (Danio rerio).
Laing LV, Viana J, Dempster EL, Trznadel M, Trunkfield LA, Uren Webster TM, van Aerle R, Paull GC, Wilson RJ, Mill J, Santos EM
Epigenetics11(7)526-38(2016 07)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Specific NuRD components are required for fin regeneration in zebrafish.
Pfefferli C, M?ller F, Ja?wi?ska A, Wicky C
BMC biology1230(2014 Apr)

Mirror movement-like defects in startle behavior of zebrafish dcc mutants are caused by aberrant midline guidance of identified descending hindbrain neurons.
Jain RA, Bell H, Lim A, Chien CB, Granato M
The Journal of neuroscience : the official journal of the Society for Neuroscience34(8)2898-909(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mbd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mbd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33542	NM_212768.1 Latest version!	Danio rerio methyl-CpG binding domain protein 2 (mbd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mbd2 ( NM_212768.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997933.1 Danio rerio methyl-CpG binding domain protein 2 (mbd2), mRNA.

Price & Availability↑

CloneID	ODa33542
Clone ID Related Accession (Same CDS sequence)	NM_212768.1
Accession Version	NM_212768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	methyl-CpG-binding domain protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY238336.1. ##Evidence-Data-START## Transcript exon combination :: AY238336.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAAGA AGAGGACGGA CTGCCCCGCG CTTCCTCCCG GCTGGAAGAA GGAAGAAGTC 
ATCCGGAAGT CAGGGCTGAG CGCTGGGAAG AGTGATGTGT ATTATTACAG TCCCAAAGGG 
AAGAAGTTTC GCAGTAAGCC TCAGTTATCT CGATATCTGG GTAACACAGT GGATCTGGGA 
TGTTTCGACT TCCGCACGGG GAAGATGATG CCCGGCAAGA TGCAGAAGAG CAAACAGAGA 
CTCCGCAACG ACAATCTGAG CCAGAGTAAA GGTAAGCCGG ATCTGAACAC AGCACTGCCC 
ATCAGACAAA CAGCCTCCAT CTTCAAGCAG CCGGTCACTA AAGTGGTCAA CCATCCCAAC 
AACAAGGTCA AATCTGACCT GCAGAGGGCC ACAGAGCAGC CACGACAGCT GTTCTGGGAG 
AAGCGTCTGA AGGGTTTGCG CTCGTGGGAT GTGTCTGAAG AAGTGCTGAG GACGATGGAT 
CTGCCTACAG GTCTCCAGAG TATCGGTCCG GACTCGTCAG ATGAGACTCT TCTGTCTGCT 
ATCGCCAGCG CCCTCCACAT GAGCTCAGCG CCGATCACCG GTCAGACGTC CAGCGCTGCA 
GAGAAAAACC CCTCAATCTG GCTCAACACC ACACAACCAC TCTGCAAAGC GTTCAGTGTT 
ACAGACGAGC ACATACGGGA GCAGGAGCTG AAGGTCCAAC AGGCGCGCCG CAGTCTGGAG 
GAGGCTCTGA TGGCGGACGG TCTGGCCCGC GCTGCAGAAT CCACAGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997933.1
CDS	283..1053
Translation	MEKKRTDCPALPPGWKKEEVIRKSGLSAGKSDVYYYSPKGKKFRSKPQLSRYLGNTVDLGCFDFRTGKMMPGKMQKSKQRLRNDNLSQSKGKPDLNTALPIRQTASIFKQPVTKVVNHPNNKVKSDLQRATEQPRQLFWEKRLKGLRSWDVSEEVLRTMDLPTGLQSIGPDSSDETLLSAIASALHMSSAPITGQTSSAAEKNPSIWLNTTQPLCKAFSVTDEHIREQELKVQQARRSLEEALMADGLARAAESTV

Target ORF information:

RefSeq Version	NM_212768.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methyl-CpG binding domain protein 2 (mbd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212768.1

ORF Insert Sequence:

ATGGAGAAGA AGAGGACGGA CTGCCCCGCG CTTCCTCCCG GCTGGAAGAA GGAAGAAGTC 
ATCCGGAAGT CAGGGCTGAG CGCTGGGAAG AGTGATGTGT ATTATTACAG TCCCAAAGGG 
AAGAAGTTTC GCAGTAAGCC TCAGTTATCT CGATATCTGG GTAACACAGT GGATCTGGGA 
TGTTTCGACT TCCGCACGGG GAAGATGATG CCCGGCAAGA TGCAGAAGAG CAAACAGAGA 
CTCCGCAACG ACAATCTGAG CCAGAGTAAA GGTAAGCCGG ATCTGAACAC AGCACTGCCC 
ATCAGACAAA CAGCCTCCAT CTTCAAGCAG CCGGTCACTA AAGTGGTCAA CCATCCCAAC 
AACAAGGTCA AATCTGACCT GCAGAGGGCC ACAGAGCAGC CACGACAGCT GTTCTGGGAG 
AAGCGTCTGA AGGGTTTGCG CTCGTGGGAT GTGTCTGAAG AAGTGCTGAG GACGATGGAT 
CTGCCTACAG GTCTCCAGAG TATCGGTCCG GACTCGTCAG ATGAGACTCT TCTGTCTGCT 
ATCGCCAGCG CCCTCCACAT GAGCTCAGCG CCGATCACCG GTCAGACGTC CAGCGCTGCA 
GAGAAAAACC CCTCAATCTG GCTCAACACC ACACAACCAC TCTGCAAAGC GTTCAGTGTT 
ACAGACGAGC ACATACGGGA GCAGGAGCTG AAGGTCCAAC AGGCGCGCCG CAGTCTGGAG 
GAGGCTCTGA TGGCGGACGG TCTGGCCCGC GCTGCAGAAT CCACAGTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bisphenol A causes reproductive toxicity, decreases dnmt1 transcription, and reduces global DNA methylation in breeding zebrafish (Danio rerio).
Epigenetics11(7)526-38(2016 07)
Laing LV,Viana J,Dempster EL,Trznadel M,Trunkfield LA,Uren Webster TM,van Aerle R,Paull GC,Wilson RJ,Mill J,Santos EM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Specific NuRD components are required for fin regeneration in zebrafish.
BMC biology1230(2014 Apr)
Pfefferli C,M?ller F,Ja?wi?ska A,Wicky C

Mirror movement-like defects in startle behavior of zebrafish dcc mutants are caused by aberrant midline guidance of identified descending hindbrain neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(8)2898-909(2014 Feb)
Jain RA,Bell H,Lim A,Chien CB,Granato M