TMF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMF1
Entrez Gene ID	445128
Full Name	TATA element modulatory factor 1
Synonyms	zgc:100899
General protein information	Preferred Names TATA element modulatory factor 1 Names TATA element modulatory factor
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003522.2	NP_001003522.2	TATA element modulatory factor
XM_021479595.1	XP_021335270.1	TATA element modulatory factor isoform X1
XM_005155803.3	XP_005155860.1	TATA element modulatory factor isoform X1

Pathways from BioSystems

Homologies

	TMF1	XP_001088711.2
Danio rerio (zebrafish)	tmf1	NP_001003522.1
Canis lupus familiaris (dog)	TMF1	XP_005632331.1
Homo sapiens (human)	TMF1	NP_009045.2
Bos taurus (cattle)	TMF1	NP_001193189.1
Pan troglodytes (chimpanzee)	TMF1	XP_526227.2
Mus musculus (house mouse)	Tmf1	NP_001074580.1
Rattus norvegicus (Norway rat)	Tmf1	NP_446123.2
Gallus gallus (chicken)	TMF1	XP_423749.2

Gene Ontology
Bibliography

Related articles in PubMed

The Golgi matrix protein giantin is required for normal cilia function in zebrafish.
Bergen DJM, Stevenson NL, Skinner REH, Stephens DJ, Hammond CL
Biology open6(8)1180-1189(2017 Aug)

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
Liongue C, O'Sullivan LA, Trengove MC, Ward AC
PloS one7(3)e32777(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TMF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa60472	NM_001003522.2 Latest version!	Danio rerio TATA element modulatory factor 1 (tmf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa16942	XM_021479595.1 Latest version!	Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa16942	XM_005155803.3 Latest version!	Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMF1 ( NM_001003522.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001003522.2 Danio rerio TATA element modulatory factor 1 (tmf1), mRNA.

Price & Availability↑

CloneID	ODa60472
Clone ID Related Accession (Same CDS sequence)	NM_001003522.2
Accession Version	NM_001003522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-14
Organism	Danio rerio(zebrafish)
Product	TATA element modulatory factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954814.17 and EB929950.1. On Sep 4, 2015 this sequence version replaced NM_001003522.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01041311.1, BC077134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGCAGGAGGA CTCGCTGGAC 
CATTCTTTCA ACACTATGAC CATGTCCATC AGCGGGACTA ACCTGTATGA AGCGGCTCGT 
CTGGGTGGTG GATCCAGTGT CATTGAGAGC CTACAGTCTC AGCTCAAGCT CAGAGAGGGT 
GAAATAACAC AGCTGCAGAT GGAGATTGCT AGTCTAGAGC GAAGTCGCAC TGTGATGTCA 
GAAGAGCTGA TCCGATTGAC CAATGAGAAC GATGAAATGG AGAGTAAAGT GAAGGAAATC 
CCTAGGCTGA AAGTGCAACT GAAGGATCTT GAACAAAGGC ACAACACAAT CCTGCAGATG 
TATGGCGAGA AGGCAGAGGA GGCTGAAGAG CTGCGTCTGG ACCTTGAGGA TGTAAAGAAC 
ATGTACAAAC TACAAATAGA TGAACTGCTG AAAAACCAGA AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003522.2
CDS	386..3793
Translation	MSWFNASHLSSFAKQALTTAQKSIDRVLDIKEEEWGDTTGQNFPDPPGKPAPTGWGMNQWNSPTEEQTTSPPPSSEAITTPVTRTVVDESEDFFSAFLPPGDVQAVSNTKVVSVAPAKSNRRLREKADKDKELVDNDLQGQAELSKSSSQEITEIKPAGAELSEQNSSTVEEQMQPELGIPASGVAPEQAVEQESIEDQTESDGLVLTLDVPLTNVPSFSSEGESVDSFDPPLNVETKNPDKTLPVPPKEIILESKDAKSEDRQSDTPSPPVSAFSSGTSTTSDIEVLDHESVLSESSASSRQETAEAKAGLHLMQGSFQLLSASTCSDFPRLDEYPKLTESCGSSSDAFERIDSFSVQSLDSRSVSEVNSDDEIPGNRTLASITAGPLPAPAESVPQTKQEEEEGMIESMNDQSLDDNEMEESGRSATPVNCEQVDELQEVDQEPEPDLTMSSQKIDVHETVTPLSTEEKPGDSPFQISELQKIIEELSGRLEKRESQLLAVSKDKARLEEECDNLKDEVISLKEESSSVQSLKDEFTQRIADAERKAQLSCKERDIAKKEIKGLREELASRLNSNETLELIKEKEEQIRELLEEGEKLSKQQLQHSNIIKKLRVKERESDAQIAKQTKKLKEQEEELKLLQQVMDGKEEIEKQHRENIKKLNAVVERQEKELSKLQTSSEELQEKSRSLQAALDSSYKELAELHKANASKDSEAQELALSREVQAKEELSLALEKVQEDSRLQQEALANQVADLRVALQRAEQQQAKKEDYLREEISELQQRIQEAETRNQELSQSVTSATRPLLRQIENLQATLGAQTASWEKLEKNLSDRLADAQAQLAVSVEKERSATEELLAIRAQITSFESQASLLRQEKGRLQGQLDAERMRREKLEDDLSRERVELENLKGEYARVSEDSKKEKLLLTNQLEMEKMKVEQEKKKCYLAQEALKEKERKSHQSVTDAPASSTPSLSRSSSVSGADHAGLPSSFFSQQEDSLDHSFNTMTMSISGTNLYEAARLGGGSSVIESLQSQLKLREGEITQLQMEIASLERSRTVMSEELIRLTNENDEMESKVKEIPRLKVQLKDLEQRHNTILQMYGEKAEEAEELRLDLEDVKNMYKLQIDELLKNQKQ

Target ORF information:

RefSeq Version	NM_001003522.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TATA element modulatory factor 1 (tmf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003522.2

ORF Insert Sequence:

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGCAGGAGGA CTCGCTGGAC 
CATTCTTTCA ACACTATGAC CATGTCCATC AGCGGGACTA ACCTGTATGA AGCGGCTCGT 
CTGGGTGGTG GATCCAGTGT CATTGAGAGC CTACAGTCTC AGCTCAAGCT CAGAGAGGGT 
GAAATAACAC AGCTGCAGAT GGAGATTGCT AGTCTAGAGC GAAGTCGCAC TGTGATGTCA 
GAAGAGCTGA TCCGATTGAC CAATGAGAAC GATGAAATGG AGAGTAAAGT GAAGGAAATC 
CCTAGGCTGA AAGTGCAACT GAAGGATCTT GAACAAAGGC ACAACACAAT CCTGCAGATG 
TATGGCGAGA AGGCAGAGGA GGCTGAAGAG CTGCGTCTGG ACCTTGAGGA TGTAAAGAAC 
ATGTACAAAC TACAAATAGA TGAACTGCTG AAAAACCAGA AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMF1 ( XM_021479595.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021335270.1 Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa16942
Clone ID Related Accession (Same CDS sequence)	XM_021479595.1 , XM_005155803.3
Accession Version	XM_021479595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TATA element modulatory factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGGAGGACTC GCTGGACCAT 
TCTTTCAACA CTATGACCAT GTCCATCAGC GGGACTAACC TGTATGAAGC GGCTCGTCTG 
GGTGGTGGAT CCAGTGTCAT TGAGAGCCTA CAGTCTCAGC TCAAGCTCAG AGAGGGTGAA 
ATAACACAGC TGCAGATGGA GATTGCTAGT CTAGAGCGAA GTCGCACTGT GATGTCAGAA 
GAGCTGATCC GATTGACCAA TGAGAACGAT GAAATGGAGA GTAAAGTGAA GGAAATCCCT 
AGGCTGAAAG TGCAACTGAA GGATCTTGAA CAAAGGCACA ACACAATCCT GCAGATGTAT 
GGCGAGAAGG CAGAGGAGGC TGAAGAGCTG CGTCTGGACC TTGAGGATGT AAAGAACATG 
TACAAACTAC AAATAGATGA ACTGCTGAAA AACCAGAAAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335270.1
CDS	385..3789
Translation	MSWFNASHLSSFAKQALTTAQKSIDRVLDIKEEEWGDTTGQNFPDPPGKPAPTGWGMNQWNSPTEEQTTSPPPSSEAITTPVTRTVVDESEDFFSAFLPPGDVQAVSNTKVVSVAPAKSNRRLREKADKDKELVDNDLQGQAELSKSSSQEITEIKPAGAELSEQNSSTVEEQMQPELGIPASGVAPEQAVEQESIEDQTESDGLVLTLDVPLTNVPSFSSEGESVDSFDPPLNVETKNPDKTLPVPPKEIILESKDAKSEDRQSDTPSPPVSAFSSGTSTTSDIEVLDHESVLSESSASSRQETAEAKAGLHLMQGSFQLLSASTCSDFPRLDEYPKLTESCGSSSDAFERIDSFSVQSLDSRSVSEVNSDDEIPGNRTLASITAGPLPAPAESVPQTKQEEEEGMIESMNDQSLDDNEMEESGRSATPVNCEQVDELQEVDQEPEPDLTMSSQKIDVHETVTPLSTEEKPGDSPFQISELQKIIEELSGRLEKRESQLLAVSKDKARLEEECDNLKDEVISLKEESSSVQSLKDEFTQRIADAERKAQLSCKERDIAKKEIKGLREELASRLNSNETLELIKEKEEQIRELLEEGEKLSKQQLQHSNIIKKLRVKERESDAQIAKQTKKLKEQEEELKLLQQVMDGKEEIEKQHRENIKKLNAVVERQEKELSKLQTSSEELQEKSRSLQAALDSSYKELAELHKANASKDSEAQELALSREVQAKEELSLALEKVQEDSRLQQEALANQVADLRVALQRAEQQQAKKEDYLREEISELQQRIQEAETRNQELSQSVTSATRPLLRQIENLQATLGAQTASWEKLEKNLSDRLADAQAQLAVSVEKERSATEELLAIRAQITSFESQASLLRQEKGRLQGQLDAERMRREKLEDDLSRERVELENLKGEYARVSEDSKKEKLLLTNQLEMEKMKVEQEKKKCYLAQEALKEKERKSHQSVTDAPASSTPSLSRSSSVSGADHAGLPSSFFSQEDSLDHSFNTMTMSISGTNLYEAARLGGGSSVIESLQSQLKLREGEITQLQMEIASLERSRTVMSEELIRLTNENDEMESKVKEIPRLKVQLKDLEQRHNTILQMYGEKAEEAEELRLDLEDVKNMYKLQIDELLKNQKQ

Target ORF information:

RefSeq Version	XM_021479595.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479595.1

ORF Insert Sequence:

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGGAGGACTC GCTGGACCAT 
TCTTTCAACA CTATGACCAT GTCCATCAGC GGGACTAACC TGTATGAAGC GGCTCGTCTG 
GGTGGTGGAT CCAGTGTCAT TGAGAGCCTA CAGTCTCAGC TCAAGCTCAG AGAGGGTGAA 
ATAACACAGC TGCAGATGGA GATTGCTAGT CTAGAGCGAA GTCGCACTGT GATGTCAGAA 
GAGCTGATCC GATTGACCAA TGAGAACGAT GAAATGGAGA GTAAAGTGAA GGAAATCCCT 
AGGCTGAAAG TGCAACTGAA GGATCTTGAA CAAAGGCACA ACACAATCCT GCAGATGTAT 
GGCGAGAAGG CAGAGGAGGC TGAAGAGCTG CGTCTGGACC TTGAGGATGT AAAGAACATG 
TACAAACTAC AAATAGATGA ACTGCTGAAA AACCAGAAAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMF1 ( XM_005155803.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155860.1 Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.

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CloneID	ODa16942
Clone ID Related Accession (Same CDS sequence)	XM_021479595.1 , XM_005155803.3
Accession Version	XM_005155803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TATA element modulatory factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGGAGGACTC GCTGGACCAT 
TCTTTCAACA CTATGACCAT GTCCATCAGC GGGACTAACC TGTATGAAGC GGCTCGTCTG 
GGTGGTGGAT CCAGTGTCAT TGAGAGCCTA CAGTCTCAGC TCAAGCTCAG AGAGGGTGAA 
ATAACACAGC TGCAGATGGA GATTGCTAGT CTAGAGCGAA GTCGCACTGT GATGTCAGAA 
GAGCTGATCC GATTGACCAA TGAGAACGAT GAAATGGAGA GTAAAGTGAA GGAAATCCCT 
AGGCTGAAAG TGCAACTGAA GGATCTTGAA CAAAGGCACA ACACAATCCT GCAGATGTAT 
GGCGAGAAGG CAGAGGAGGC TGAAGAGCTG CGTCTGGACC TTGAGGATGT AAAGAACATG 
TACAAACTAC AAATAGATGA ACTGCTGAAA AACCAGAAAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155860.1
CDS	383..3787
Translation	MSWFNASHLSSFAKQALTTAQKSIDRVLDIKEEEWGDTTGQNFPDPPGKPAPTGWGMNQWNSPTEEQTTSPPPSSEAITTPVTRTVVDESEDFFSAFLPPGDVQAVSNTKVVSVAPAKSNRRLREKADKDKELVDNDLQGQAELSKSSSQEITEIKPAGAELSEQNSSTVEEQMQPELGIPASGVAPEQAVEQESIEDQTESDGLVLTLDVPLTNVPSFSSEGESVDSFDPPLNVETKNPDKTLPVPPKEIILESKDAKSEDRQSDTPSPPVSAFSSGTSTTSDIEVLDHESVLSESSASSRQETAEAKAGLHLMQGSFQLLSASTCSDFPRLDEYPKLTESCGSSSDAFERIDSFSVQSLDSRSVSEVNSDDEIPGNRTLASITAGPLPAPAESVPQTKQEEEEGMIESMNDQSLDDNEMEESGRSATPVNCEQVDELQEVDQEPEPDLTMSSQKIDVHETVTPLSTEEKPGDSPFQISELQKIIEELSGRLEKRESQLLAVSKDKARLEEECDNLKDEVISLKEESSSVQSLKDEFTQRIADAERKAQLSCKERDIAKKEIKGLREELASRLNSNETLELIKEKEEQIRELLEEGEKLSKQQLQHSNIIKKLRVKERESDAQIAKQTKKLKEQEEELKLLQQVMDGKEEIEKQHRENIKKLNAVVERQEKELSKLQTSSEELQEKSRSLQAALDSSYKELAELHKANASKDSEAQELALSREVQAKEELSLALEKVQEDSRLQQEALANQVADLRVALQRAEQQQAKKEDYLREEISELQQRIQEAETRNQELSQSVTSATRPLLRQIENLQATLGAQTASWEKLEKNLSDRLADAQAQLAVSVEKERSATEELLAIRAQITSFESQASLLRQEKGRLQGQLDAERMRREKLEDDLSRERVELENLKGEYARVSEDSKKEKLLLTNQLEMEKMKVEQEKKKCYLAQEALKEKERKSHQSVTDAPASSTPSLSRSSSVSGADHAGLPSSFFSQEDSLDHSFNTMTMSISGTNLYEAARLGGGSSVIESLQSQLKLREGEITQLQMEIASLERSRTVMSEELIRLTNENDEMESKVKEIPRLKVQLKDLEQRHNTILQMYGEKAEEAEELRLDLEDVKNMYKLQIDELLKNQKQ

Target ORF information:

RefSeq Version	XM_005155803.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TATA element modulatory factor 1 (tmf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155803.3

ORF Insert Sequence:

ATGAGTTGGT TCAACGCTTC ACATCTGTCG AGTTTTGCCA AACAAGCTTT GACAACTGCT 
CAGAAATCTA TCGACAGGGT CCTGGACATT AAAGAAGAGG AGTGGGGTGA CACAACCGGA 
CAGAACTTCC CTGATCCACC AGGCAAGCCA GCACCTACGG GATGGGGAAT GAACCAGTGG 
AACTCGCCTA CTGAGGAACA AACCACGAGT CCCCCTCCAT CCTCTGAGGC CATCACAACG 
CCTGTCACAC GAACAGTGGT TGATGAATCT GAGGATTTCT TCAGTGCCTT TTTGCCTCCT 
GGGGATGTAC AAGCTGTAAG CAACACTAAG GTAGTGTCTG TGGCACCTGC CAAGTCAAAC 
CGCAGACTAC GGGAAAAAGC AGACAAGGAC AAGGAGTTAG TGGACAATGA CTTACAAGGT 
CAGGCAGAGC TCAGTAAAAG CAGCAGCCAG GAAATAACTG AGATAAAGCC AGCCGGGGCA 
GAACTTTCAG AGCAGAATTC ATCCACTGTG GAAGAGCAGA TGCAACCAGA ACTGGGTATT 
CCTGCTTCAG GTGTTGCACC AGAACAAGCT GTAGAACAGG AGTCGATCGA GGACCAAACA 
GAGTCAGATG GTTTGGTTTT AACTTTAGAT GTTCCACTCA CAAATGTACC TTCATTTTCC 
TCAGAGGGTG AATCTGTTGA TTCGTTTGAT CCACCTTTGA ACGTCGAGAC CAAAAATCCA 
GATAAAACCC TGCCTGTCCC TCCTAAAGAA ATTATTTTAG AATCCAAAGA TGCTAAATCA 
GAGGACAGAC AAAGCGACAC CCCTTCTCCT CCTGTTAGTG CCTTCTCCTC TGGAACTTCC 
ACTACCAGCG ACATTGAGGT TTTGGACCAT GAGAGTGTGC TGAGCGAGAG CTCGGCCAGC 
TCCAGACAGG AAACAGCTGA GGCTAAAGCA GGTCTGCACC TGATGCAAGG CTCATTCCAG 
CTTCTCTCCG CTTCAACATG CAGCGACTTC CCTCGCCTGG ATGAATATCC CAAACTCACT 
GAAAGCTGCG GCTCCTCATC TGATGCTTTT GAGCGCATTG ACTCGTTTAG CGTGCAGTCG 
CTGGACAGCC GCAGTGTCAG TGAAGTGAAT TCTGATGATG AGATCCCAGG GAACCGTACA 
TTGGCCTCCA TCACAGCTGG ACCTTTGCCT GCTCCGGCTG AGTCTGTGCC ACAGACAAAA 
CAGGAGGAAG AGGAGGGTAT GATTGAGTCA ATGAATGACC AATCGCTGGA TGACAATGAG 
ATGGAGGAAA GTGGCCGCAG TGCAACACCA GTGAACTGTG AGCAGGTAGA TGAATTACAA 
GAAGTCGACC AGGAACCTGA ACCTGACCTC ACAATGTCCA GTCAAAAAAT CGATGTACAT 
GAAACTGTAA CACCACTGAG TACTGAAGAA AAACCTGGAG ATTCACCCTT CCAGATTTCA 
GAGCTCCAAA AGATTATTGA GGAGCTCTCT GGCCGATTGG AAAAGAGAGA ATCACAGTTG 
CTTGCGGTCA GTAAAGACAA GGCCAGACTT GAGGAGGAGT GTGACAATCT CAAAGATGAG 
GTGATCAGCT TGAAGGAAGA AAGCTCTTCT GTACAATCTT TAAAAGACGA GTTCACCCAG 
CGCATTGCTG ATGCAGAGAG GAAAGCTCAA CTCTCCTGCA AAGAGAGAGA CATCGCAAAA 
AAGGAAATCA AAGGCCTCAG AGAAGAACTG GCAAGCAGGT TAAACTCAAA TGAAACTTTG 
GAACTGATTA AAGAGAAAGA AGAACAGATC CGAGAGCTTC TTGAAGAAGG TGAGAAACTG 
TCCAAGCAGC AGTTGCAGCA CTCCAACATC ATTAAGAAAC TGCGTGTGAA AGAGAGGGAG 
AGTGATGCAC AAATCGCCAA ACAGACCAAA AAACTTAAAG AACAGGAAGA AGAACTGAAA 
CTCTTGCAGC AGGTTATGGA TGGTAAGGAG GAAATAGAAA AGCAGCACAG AGAGAACATC 
AAGAAGCTGA ATGCTGTGGT GGAGCGGCAG GAGAAGGAAC TAAGTAAACT GCAGACCAGC 
AGCGAGGAGC TTCAGGAAAA GAGCCGCAGC TTGCAAGCAG CTCTCGACAG CTCCTACAAG 
GAGTTGGCTG AACTCCACAA GGCCAACGCC TCGAAGGACA GTGAAGCACA GGAGCTTGCG 
CTGAGCAGAG AGGTGCAGGC AAAAGAAGAG CTGAGTTTGG CTTTGGAGAA AGTCCAAGAA 
GATTCCCGCC TACAACAAGA GGCACTGGCC AATCAGGTGG CTGATCTGAG GGTGGCTCTA 
CAGAGAGCTG AACAGCAGCA GGCCAAGAAG GAAGATTACC TGAGAGAGGA GATCAGTGAG 
TTACAGCAGA GGATACAAGA AGCAGAGACA AGAAACCAGG AGTTGAGTCA AAGTGTAACA 
TCAGCAACAC GGCCGCTCCT TCGACAGATA GAGAACCTTC AGGCCACCCT TGGGGCTCAG 
ACAGCATCAT GGGAAAAACT GGAGAAGAAC CTTTCTGACC GCTTAGCTGA TGCCCAGGCT 
CAGCTTGCTG TTTCAGTAGA GAAAGAGCGT TCAGCTACAG AAGAACTGCT AGCCATCCGA 
GCACAGATAA CATCTTTTGA GTCCCAGGCC TCTTTATTGA GGCAGGAGAA GGGGCGTCTG 
CAGGGACAGC TGGATGCAGA GAGGATGAGG AGGGAAAAAC TGGAGGATGA CCTAAGCAGG 
GAACGTGTAG AGCTGGAGAA TCTTAAAGGG GAATATGCTC GAGTATCAGA AGACTCCAAG 
AAGGAGAAGT TGCTTCTCAC TAACCAACTC GAAATGGAGA AAATGAAGGT GGAACAGGAG 
AAGAAGAAAT GCTACCTTGC ACAAGAGGCC CTCAAAGAAA AGGAGAGGAA GTCTCATCAG 
TCTGTGACTG ATGCTCCAGC TTCCTCCACT CCTTCACTGT CCCGCTCCAG TTCTGTTAGT 
GGAGCCGATC ATGCCGGATT ACCTTCCTCC TTCTTCTCAC AGGAGGACTC GCTGGACCAT 
TCTTTCAACA CTATGACCAT GTCCATCAGC GGGACTAACC TGTATGAAGC GGCTCGTCTG 
GGTGGTGGAT CCAGTGTCAT TGAGAGCCTA CAGTCTCAGC TCAAGCTCAG AGAGGGTGAA 
ATAACACAGC TGCAGATGGA GATTGCTAGT CTAGAGCGAA GTCGCACTGT GATGTCAGAA 
GAGCTGATCC GATTGACCAA TGAGAACGAT GAAATGGAGA GTAAAGTGAA GGAAATCCCT 
AGGCTGAAAG TGCAACTGAA GGATCTTGAA CAAAGGCACA ACACAATCCT GCAGATGTAT 
GGCGAGAAGG CAGAGGAGGC TGAAGAGCTG CGTCTGGACC TTGAGGATGT AAAGAACATG 
TACAAACTAC AAATAGATGA ACTGCTGAAA AACCAGAAAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Golgi matrix protein giantin is required for normal cilia function in zebrafish.
Biology open6(8)1180-1189(2017 Aug)
Bergen DJM,Stevenson NL,Skinner REH,Stephens DJ,Hammond CL

Evolution of JAK-STAT pathway components: mechanisms and role in immune system development.
PloS one7(3)e32777(2012)
Liongue C,O'Sullivan LA,Trengove MC,Ward AC

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TMF1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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