opa1 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following opa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the opa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa02750 NM_001007298.1
Latest version!
Danio rerio OPA1 mitochondrial dynamin like GTPase (opa1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa12005 XM_021476849.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa142955 XM_021476850.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa59356 XM_017356479.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa59357 XM_017356480.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa12003 XM_017356477.2
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa12004 XM_021476847.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa142954 XM_021476848.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa59353 XM_017356475.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa59353 XM_017356476.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa59354 XM_017356477.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa59355 XM_017356478.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa12002 XM_021476845.1
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa12002 XM_009302514.3
Latest version!
Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa12002 XM_009302514.2 Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa02750
    Clone ID Related Accession (Same CDS sequence) NM_001007298.1
    Accession Version NM_001007298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2901bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085633.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085633.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGTCATCCGA TAAAGAGAAA
    GTTGACCAGC TTCAGGAGGA ACTTCTTCGA ACTCAGTTGA AGTACCAACG GATGCTTGAG
    CGCTTGGAAA AGGAAAATAA AGAATTGAGG AAAGTTGTAT TGCAGAAGGA TGACAAGGGT
    ATTCACCAAA GAAAAGTCAA GAAATCTTTG ATTGATATGT ACTCTGAGGT CCTGGACATT
    CTTTCAGATT ATGACTCCAA CTACAACACT CAAGATCACC TGCCAAGGGT GGTGGTAGTT
    GGGGACCAGA GTGCGGGAAA GACTAGTGTG CTGGAGATGA TTGCTCAAGC TAGGATCTTT
    CCGAGAGGCT CTGGAGAAAT GATGACAAGG TCTCCTGTCA AGGTGACTTT GAGTGAAGGC
    CCTCACCATG TAGCAATGTT TAAAGACAGC TCTCGGGAGT TTGACCTTGG CAAAGAGGAA
    GATCTTGCAG CACTGAGGCA TGAGATTGAG CTCAGGATGA GGAAGAGTGT GAAGGAAGGT
    CAGACAGTCA GCCCTGAGAC CATCTCTTTA AGTGTTAAGG GCCCTGGAAT CCAGCGAATG
    GTGCTTGTAG ATTTACCAGG GGTCATCAGT ACTGTGACAA CTGGAATGGC CGCAGACACA
    AAGGAAACCA TTTTTAGCAT TAGTAAAGCT TACATGCAGA ATCCAAACGC CATCATCCTC
    TGTATACAGG ATGGCTCAGT AGATGCAGAG CGCAGTATAG TCACTGATCT GGTCAGTCAA
    ATGGACCCTC AGGGCAAAAG GACCATATTT GTTCTTACTA AGGTTGATCT GGCAGAAAAG
    AACCTGGCTA GTCCAAGCAG AATCCAGCAA ATAGTAGAAG GCAAATTATT CCCAATGAAG
    GCTTTGGGCT ACTTTGCTGT AGTAACTGGC AAAGGCAGCC CTAATGAGAG CATAGATTCC
    ATTAAAGACT ATGAGGAGGA CTTCTTCCAG AACTCAAGAC TGCTGAAGGA TGGCATGTTG
    AAAGCTCATC AGGTGACTAC AAAGAACCTG AGTCTTGCTG TGTCAGACTG TTTCTGGAAA
    ATGGTCAGAG AGTCAGTAGA GCAACAAGCA GATGCCTTTA AAGCTTCTCG ATTTAACCTG
    GAGACTGAGT GGAAGAACAA CTATCCTCGC TTACGAGAGC TGGACAGGAA CGAGCTCTAC
    GAGAAGGCCA AAAATGAGAT CTTGGATGAG GTGATCAGCT TGAGTCAAGT TACACCAAAA
    CACTGGGAGT CTATCTTACA GAAGAAGCTT TGGGAAAGAG TCTCGACACA CGTCATAGAG
    AACATCTACC TGCCTGCTGC ACAGACCATG AACTCTGGAA CTTTTAACAC CACTGTAGAC
    ATCAAACTCA AGCAATGGAC TGATAAACAA CTTCCTCATA AAGCACTAGA GGTTGCTTGG
    GAGACTTTGC AGGAAGAGTT TGCCCGCTTC ATGGCCGAAT ACAAAGGGAA AGACCAGGAT
    GACATTTTTG ACAAGCTGAA GGAAGCTGTC AAGGACGAGA GCATCAAACG ACACAAGTGG
    AATGAAAGAG CCATGGACAG CTTGAGAGTG ATTCAACACA ACGCTCTGGA GGATCGTTCA
    ATCACAGACA AGCCCCAGTG GGATGCCGCT ATACAGTTCA TGGAGGAAAC GCTGCAGTCT
    CGTCTTAAAG ACACTGAGTC AGTAATAGCT GATATGGTGG GGCCAGACTG GAAGCAGAGG
    TGGATGAGCT GGAAAAACCG AACACCAGAG CAGCACACCC GGAATGAAAC CAAAAATGAG
    CTGGAGCGTC TGCTGAAACT GCATGAGGAT CACACAGCAT ATTTGGCCAA TGATGAGGTC
    ACTACAGTGC GCAAAAACCT GGAGGCCCGA GGGGTGGAGG TGGACCCTGT ATTGATTAAG
    GACACATGGC ATCAGCTCTT TCGACGTCAC TTCCTACAGA AGGCACTACT ACATTGCAAC
    CTCTGCAGAC GAGGTTTCTA CTACTATCAG AGGCACTTCG TAGACTCTGA GCTTGAGTGC
    AATGATGTGG TTCTGTTCTG GAGGATTCAG AGGATGTTGG GCATCACTGC CAATACTCTT
    CGACAACAGC TCACTAACAC TGAGGTACGG CGTTTGGAGA AGAACGTTAA AGAAGTATTA
    GAAGACTTTG GAGAGGACAA TGAGAAAAAA GTCCAGCTGA TCACAGGCAG GAGGGTTCAA
    CTGGCAGAAG ACCTCAAAAA AGTTCGCGAA ATTCAGGAGA AACTTGAAGC CTTCATCGAA
    GCTCTTCACA AAGAGAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007299.1
    CDS112..3012
    Translation

    Target ORF information:

    RefSeq Version NM_001007298.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio OPA1 mitochondrial dynamin like GTPase (opa1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001007298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGTCATCCGA TAAAGAGAAA
    GTTGACCAGC TTCAGGAGGA ACTTCTTCGA ACTCAGTTGA AGTACCAACG GATGCTTGAG
    CGCTTGGAAA AGGAAAATAA AGAATTGAGG AAAGTTGTAT TGCAGAAGGA TGACAAGGGT
    ATTCACCAAA GAAAAGTCAA GAAATCTTTG ATTGATATGT ACTCTGAGGT CCTGGACATT
    CTTTCAGATT ATGACTCCAA CTACAACACT CAAGATCACC TGCCAAGGGT GGTGGTAGTT
    GGGGACCAGA GTGCGGGAAA GACTAGTGTG CTGGAGATGA TTGCTCAAGC TAGGATCTTT
    CCGAGAGGCT CTGGAGAAAT GATGACAAGG TCTCCTGTCA AGGTGACTTT GAGTGAAGGC
    CCTCACCATG TAGCAATGTT TAAAGACAGC TCTCGGGAGT TTGACCTTGG CAAAGAGGAA
    GATCTTGCAG CACTGAGGCA TGAGATTGAG CTCAGGATGA GGAAGAGTGT GAAGGAAGGT
    CAGACAGTCA GCCCTGAGAC CATCTCTTTA AGTGTTAAGG GCCCTGGAAT CCAGCGAATG
    GTGCTTGTAG ATTTACCAGG GGTCATCAGT ACTGTGACAA CTGGAATGGC CGCAGACACA
    AAGGAAACCA TTTTTAGCAT TAGTAAAGCT TACATGCAGA ATCCAAACGC CATCATCCTC
    TGTATACAGG ATGGCTCAGT AGATGCAGAG CGCAGTATAG TCACTGATCT GGTCAGTCAA
    ATGGACCCTC AGGGCAAAAG GACCATATTT GTTCTTACTA AGGTTGATCT GGCAGAAAAG
    AACCTGGCTA GTCCAAGCAG AATCCAGCAA ATAGTAGAAG GCAAATTATT CCCAATGAAG
    GCTTTGGGCT ACTTTGCTGT AGTAACTGGC AAAGGCAGCC CTAATGAGAG CATAGATTCC
    ATTAAAGACT ATGAGGAGGA CTTCTTCCAG AACTCAAGAC TGCTGAAGGA TGGCATGTTG
    AAAGCTCATC AGGTGACTAC AAAGAACCTG AGTCTTGCTG TGTCAGACTG TTTCTGGAAA
    ATGGTCAGAG AGTCAGTAGA GCAACAAGCA GATGCCTTTA AAGCTTCTCG ATTTAACCTG
    GAGACTGAGT GGAAGAACAA CTATCCTCGC TTACGAGAGC TGGACAGGAA CGAGCTCTAC
    GAGAAGGCCA AAAATGAGAT CTTGGATGAG GTGATCAGCT TGAGTCAAGT TACACCAAAA
    CACTGGGAGT CTATCTTACA GAAGAAGCTT TGGGAAAGAG TCTCGACACA CGTCATAGAG
    AACATCTACC TGCCTGCTGC ACAGACCATG AACTCTGGAA CTTTTAACAC CACTGTAGAC
    ATCAAACTCA AGCAATGGAC TGATAAACAA CTTCCTCATA AAGCACTAGA GGTTGCTTGG
    GAGACTTTGC AGGAAGAGTT TGCCCGCTTC ATGGCCGAAT ACAAAGGGAA AGACCAGGAT
    GACATTTTTG ACAAGCTGAA GGAAGCTGTC AAGGACGAGA GCATCAAACG ACACAAGTGG
    AATGAAAGAG CCATGGACAG CTTGAGAGTG ATTCAACACA ACGCTCTGGA GGATCGTTCA
    ATCACAGACA AGCCCCAGTG GGATGCCGCT ATACAGTTCA TGGAGGAAAC GCTGCAGTCT
    CGTCTTAAAG ACACTGAGTC AGTAATAGCT GATATGGTGG GGCCAGACTG GAAGCAGAGG
    TGGATGAGCT GGAAAAACCG AACACCAGAG CAGCACACCC GGAATGAAAC CAAAAATGAG
    CTGGAGCGTC TGCTGAAACT GCATGAGGAT CACACAGCAT ATTTGGCCAA TGATGAGGTC
    ACTACAGTGC GCAAAAACCT GGAGGCCCGA GGGGTGGAGG TGGACCCTGT ATTGATTAAG
    GACACATGGC ATCAGCTCTT TCGACGTCAC TTCCTACAGA AGGCACTACT ACATTGCAAC
    CTCTGCAGAC GAGGTTTCTA CTACTATCAG AGGCACTTCG TAGACTCTGA GCTTGAGTGC
    AATGATGTGG TTCTGTTCTG GAGGATTCAG AGGATGTTGG GCATCACTGC CAATACTCTT
    CGACAACAGC TCACTAACAC TGAGGTACGG CGTTTGGAGA AGAACGTTAA AGAAGTATTA
    GAAGACTTTG GAGAGGACAA TGAGAAAAAA GTCCAGCTGA TCACAGGCAG GAGGGTTCAA
    CTGGCAGAAG ACCTCAAAAA AGTTCGCGAA ATTCAGGAGA AACTTGAAGC CTTCATCGAA
    GCTCTTCACA AAGAGAAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12005
    Clone ID Related Accession (Same CDS sequence) XM_021476849.1
    Accession Version XM_021476849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG TCATCCGATA AAGAGAAAGT TGACCAGCTT
    CAGGAGGAAC TTCTTCGAAC TCAGTTGAAG TACCAACGGA TGCTTGAGCG CTTGGAAAAG
    GAAAATAAAG AATTGAGGAA AGTTGTATTG CAGAAGGATG ACAAGGGTAT TCACCAAAGA
    AAAGTCAAGA AATCTTTGAT TGATATGTAC TCTGAGGTCC TGGACATTCT TTCAGATTAT
    GACTCCAACT ACAACACTCA AGATCACCTG CCAAGGGTGG TGGTAGTTGG GGACCAGAGT
    GCGGGAAAGA CTAGTGTGCT GGAGATGATT GCTCAAGCTA GGATCTTTCC GAGAGGCTCT
    GGAGAAATGA TGACAAGGTC TCCTGTCAAG GTGACTTTGA GTGAAGGCCC TCACCATGTA
    GCAATGTTTA AAGACAGCTC TCGGGAGTTT GACCTTGGCA AAGAGGAAGA TCTTGCAGCA
    CTGAGGCATG AGATTGAGCT CAGGATGAGG AAGAGTGTGA AGGAAGGTCA GACAGTCAGC
    CCTGAGACCA TCTCTTTAAG TGTTAAGGGC CCTGGAATCC AGCGAATGGT GCTTGTAGAT
    TTACCAGGGG TCATCAGTAC TGTGACAACT GGAATGGCCG CAGACACAAA GGAAACCATT
    TTTAGCATTA GTAAAGCTTA CATGCAGAAT CCAAACGCCA TCATCCTCTG TATACAGGAT
    GGCTCAGTAG ATGCAGAGCG CAGTATAGTC ACTGATCTGG TCAGTCAAAT GGACCCTCAG
    GGCAAAAGGA CCATATTTGT TCTTACTAAG GTTGATCTGG CAGAAAAGAA CCTGGCTAGT
    CCAAGCAGAA TCCAGCAAAT AGTAGAAGGC AAATTATTCC CAATGAAGGC TTTGGGCTAC
    TTTGCTGTAG TAACTGGCAA AGGCAGCCCT AATGAGAGCA TAGATTCCAT TAAAGACTAT
    GAGGAGGACT TCTTCCAGAA CTCAAGACTG CTGAAGGATG GCATGTTGAA AGCTCATCAG
    GTGACTACAA AGAACCTGAG TCTTGCTGTG TCAGACTGTT TCTGGAAAAT GGTCAGAGAG
    TCAGTAGAGC AACAAGCAGA TGCCTTTAAA GCTTCTCGAT TTAACCTGGA GACTGAGTGG
    AAGAACAACT ATCCTCGCTT ACGAGAGCTG GACAGGAACG AGCTCTACGA GAAGGCCAAA
    AATGAGATCT TGGATGAGGT GATCAGCTTG AGTCAAGTTA CACCAAAACA CTGGGAGTCT
    ATCTTACAGA AGAAGCTTTG GGAAAGAGTC TCGACACACG TCATAGAGAA CATCTACCTG
    CCTGCTGCAC AGACCATGAA CTCTGGAACT TTTAACACCA CTGTAGACAT CAAACTCAAG
    CAATGGACTG ATAAACAACT TCCTCATAAA GCACTAGAGG TTGCTTGGGA GACTTTGCAG
    GAAGAGTTTG CCCGCTTCAT GGCCGAATAC AAAGGGAAAG ACCAGGATGA CATTTTTGAC
    AAGCTGAAGG AAGCTGTCAA GGACGAGAGC ATCAAACGAC ACAAGTGGAA TGAAAGAGCC
    ATGGACAGCT TGAGAGTGAT TCAACACAAC GCTCTGGAGG ATCGTTCAAT CACAGACAAG
    CCCCAGTGGG ATGCCGCTAT ACAGTTCATG GAGGAAACGC TGCAGTCTCG TCTTAAAGAC
    ACTGAGTCAG TAATAGCTGA TATGGTGGGG CCAGACTGGA AGCAGAGGTG GATGAGCTGG
    AAAAACCGAA CACCAGAGCA GCACACCCGG AATGAAACCA AAAATGAGCT GGAGCGTCTG
    CTGAAACTGC ATGAGGATCA CACAGCATAT TTGGCCAATG ATGAGGTCAC TACAGTGCGC
    AAAAACCTGG AGGCCCGAGG GGTGGAGGTG GACCCTGTAT TGATTAAGGA CACATGGCAT
    CAGCTCTTTC GACGTCACTT CCTACAGAAG GCACTACTAC ATTGCAACCT CTGCAGACGA
    GGTTTCTACT ACTATCAGAG GCACTTCGTA GACTCTGAGC TTGAGTGCAA TGATGTGGTT
    CTGTTCTGGA GGATTCAGAG GATGTTGGGC ATCACTGCCA ATACTCTTCG ACAACAGCTC
    ACTAACACTG AGGTACGGCG TTTGGAGAAG AACGTTAAAG AAGTATTAGA AGACTTTGGA
    GAGGACAATG AGAAAAAAGT CCAGCTGATC ACAGGCAGGA GGGTTCAACT GGCAGAAGAC
    CTCAAAAAAG TTCGCGAAAT TCAGGAGAAA CTTGAAGCCT TCATTGAAGC TCTTCACAAA
    GAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332524.1
    CDS173..3121
    Translation

    Target ORF information:

    RefSeq Version XM_021476849.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG TCATCCGATA AAGAGAAAGT TGACCAGCTT
    CAGGAGGAAC TTCTTCGAAC TCAGTTGAAG TACCAACGGA TGCTTGAGCG CTTGGAAAAG
    GAAAATAAAG AATTGAGGAA AGTTGTATTG CAGAAGGATG ACAAGGGTAT TCACCAAAGA
    AAAGTCAAGA AATCTTTGAT TGATATGTAC TCTGAGGTCC TGGACATTCT TTCAGATTAT
    GACTCCAACT ACAACACTCA AGATCACCTG CCAAGGGTGG TGGTAGTTGG GGACCAGAGT
    GCGGGAAAGA CTAGTGTGCT GGAGATGATT GCTCAAGCTA GGATCTTTCC GAGAGGCTCT
    GGAGAAATGA TGACAAGGTC TCCTGTCAAG GTGACTTTGA GTGAAGGCCC TCACCATGTA
    GCAATGTTTA AAGACAGCTC TCGGGAGTTT GACCTTGGCA AAGAGGAAGA TCTTGCAGCA
    CTGAGGCATG AGATTGAGCT CAGGATGAGG AAGAGTGTGA AGGAAGGTCA GACAGTCAGC
    CCTGAGACCA TCTCTTTAAG TGTTAAGGGC CCTGGAATCC AGCGAATGGT GCTTGTAGAT
    TTACCAGGGG TCATCAGTAC TGTGACAACT GGAATGGCCG CAGACACAAA GGAAACCATT
    TTTAGCATTA GTAAAGCTTA CATGCAGAAT CCAAACGCCA TCATCCTCTG TATACAGGAT
    GGCTCAGTAG ATGCAGAGCG CAGTATAGTC ACTGATCTGG TCAGTCAAAT GGACCCTCAG
    GGCAAAAGGA CCATATTTGT TCTTACTAAG GTTGATCTGG CAGAAAAGAA CCTGGCTAGT
    CCAAGCAGAA TCCAGCAAAT AGTAGAAGGC AAATTATTCC CAATGAAGGC TTTGGGCTAC
    TTTGCTGTAG TAACTGGCAA AGGCAGCCCT AATGAGAGCA TAGATTCCAT TAAAGACTAT
    GAGGAGGACT TCTTCCAGAA CTCAAGACTG CTGAAGGATG GCATGTTGAA AGCTCATCAG
    GTGACTACAA AGAACCTGAG TCTTGCTGTG TCAGACTGTT TCTGGAAAAT GGTCAGAGAG
    TCAGTAGAGC AACAAGCAGA TGCCTTTAAA GCTTCTCGAT TTAACCTGGA GACTGAGTGG
    AAGAACAACT ATCCTCGCTT ACGAGAGCTG GACAGGAACG AGCTCTACGA GAAGGCCAAA
    AATGAGATCT TGGATGAGGT GATCAGCTTG AGTCAAGTTA CACCAAAACA CTGGGAGTCT
    ATCTTACAGA AGAAGCTTTG GGAAAGAGTC TCGACACACG TCATAGAGAA CATCTACCTG
    CCTGCTGCAC AGACCATGAA CTCTGGAACT TTTAACACCA CTGTAGACAT CAAACTCAAG
    CAATGGACTG ATAAACAACT TCCTCATAAA GCACTAGAGG TTGCTTGGGA GACTTTGCAG
    GAAGAGTTTG CCCGCTTCAT GGCCGAATAC AAAGGGAAAG ACCAGGATGA CATTTTTGAC
    AAGCTGAAGG AAGCTGTCAA GGACGAGAGC ATCAAACGAC ACAAGTGGAA TGAAAGAGCC
    ATGGACAGCT TGAGAGTGAT TCAACACAAC GCTCTGGAGG ATCGTTCAAT CACAGACAAG
    CCCCAGTGGG ATGCCGCTAT ACAGTTCATG GAGGAAACGC TGCAGTCTCG TCTTAAAGAC
    ACTGAGTCAG TAATAGCTGA TATGGTGGGG CCAGACTGGA AGCAGAGGTG GATGAGCTGG
    AAAAACCGAA CACCAGAGCA GCACACCCGG AATGAAACCA AAAATGAGCT GGAGCGTCTG
    CTGAAACTGC ATGAGGATCA CACAGCATAT TTGGCCAATG ATGAGGTCAC TACAGTGCGC
    AAAAACCTGG AGGCCCGAGG GGTGGAGGTG GACCCTGTAT TGATTAAGGA CACATGGCAT
    CAGCTCTTTC GACGTCACTT CCTACAGAAG GCACTACTAC ATTGCAACCT CTGCAGACGA
    GGTTTCTACT ACTATCAGAG GCACTTCGTA GACTCTGAGC TTGAGTGCAA TGATGTGGTT
    CTGTTCTGGA GGATTCAGAG GATGTTGGGC ATCACTGCCA ATACTCTTCG ACAACAGCTC
    ACTAACACTG AGGTACGGCG TTTGGAGAAG AACGTTAAAG AAGTATTAGA AGACTTTGGA
    GAGGACAATG AGAAAAAAGT CCAGCTGATC ACAGGCAGGA GGGTTCAACT GGCAGAAGAC
    CTCAAAAAAG TTCGCGAAAT TCAGGAGAAA CTTGAAGCCT TCATTGAAGC TCTTCACAAA
    GAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142955
    Clone ID Related Accession (Same CDS sequence) XM_021476850.1
    Accession Version XM_021476850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTCCCTG ATATGAGTGA ATACACATGG ATTGTGCCAG ACTTTGTTTG GGAGCTGAGC 
    GAAAATATAG ATTTAGACAA GCTAGCCAGT GCTTTACCTG AATTAGAAGA GATAGCCAAA
    CTACTACCTG ATATGGAGAA GATCGGAGAG AATTTCACCT TCCTGAAGAG CCTCCTCTCC
    AGCGGTTTGG GTAGTGAAGT CAATGGAGCT TCTGGTCTGC GTCTGTTATT AGAAACTACT
    GGCGAATCGG CCCTAAGAGC ACCAGACGTC CCACCAGCAT CTGCAGCCAT GGCTGACAGC
    GGAGACAAGC AGTTCAAGAA GCAGGGGCTG CTCAGCGAAC TCATTGTCAT TCAGCAGCAG
    ATCCAGCAGC ATGAGGAGGA GATGCGGCGC GCGGCAGCCA ATAACGCTCC GCCTCCGCCC
    CGTGACCCCA GTCCCAGCCC GGCCCCGAAC CCCAGCCCCT CGCAGTCCAA ACGCAAGTCA
    TCCGATAAAG AGAAAGTTGA CCAGCTTCAG GAGGAACTTC TTCGAACTCA GTTGAAGTAC
    CAACGGATGC TTGAGCGCTT GGAAAAGGAA AATAAAGAAT TGAGGAAAGT TGTATTGCAG
    AAGGATGACA AGGGTATTCA CCAAAGAAAA GTCAAGAAAT CTTTGATTGA TATGTACTCT
    GAGGTCCTGG ACATTCTTTC AGATTATGAC TCCAACTACA ACACTCAAGA TCACCTGCCA
    AGGGTGGTGG TAGTTGGGGA CCAGAGTGCG GGAAAGACTA GTGTGCTGGA GATGATTGCT
    CAAGCTAGGA TCTTTCCGAG AGGCTCTGGA GAAATGATGA CAAGGTCTCC TGTCAAGGTG
    ACTTTGAGTG AAGGCCCTCA CCATGTAGCA ATGTTTAAAG ACAGCTCTCG GGAGTTTGAC
    CTTGGCAAAG AGGAAGATCT TGCAGCACTG AGGCATGAGA TTGAGCTCAG GATGAGGAAG
    AGTGTGAAGG AAGGTCAGAC AGTCAGCCCT GAGACCATCT CTTTAAGTGT TAAGGGCCCT
    GGAATCCAGC GAATGGTGCT TGTAGATTTA CCAGGGGTCA TCAGTACTGT GACAACTGGA
    ATGGCCGCAG ACACAAAGGA AACCATTTTT AGCATTAGTA AAGCTTACAT GCAGAATCCA
    AACGCCATCA TCCTCTGTAT ACAGGATGGC TCAGTAGATG CAGAGCGCAG TATAGTCACT
    GATCTGGTCA GTCAAATGGA CCCTCAGGGC AAAAGGACCA TATTTGTTCT TACTAAGGTT
    GATCTGGCAG AAAAGAACCT GGCTAGTCCA AGCAGAATCC AGCAAATAGT AGAAGGCAAA
    TTATTCCCAA TGAAGGCTTT GGGCTACTTT GCTGTAGTAA CTGGCAAAGG CAGCCCTAAT
    GAGAGCATAG ATTCCATTAA AGACTATGAG GAGGACTTCT TCCAGAACTC AAGACTGCTG
    AAGGATGGCA TGTTGAAAGC TCATCAGGTG ACTACAAAGA ACCTGAGTCT TGCTGTGTCA
    GACTGTTTCT GGAAAATGGT CAGAGAGTCA GTAGAGCAAC AAGCAGATGC CTTTAAAGCT
    TCTCGATTTA ACCTGGAGAC TGAGTGGAAG AACAACTATC CTCGCTTACG AGAGCTGGAC
    AGGAACGAGC TCTACGAGAA GGCCAAAAAT GAGATCTTGG ATGAGGTGAT CAGCTTGAGT
    CAAGTTACAC CAAAACACTG GGAGTCTATC TTACAGAAGA AGCTTTGGGA AAGAGTCTCG
    ACACACGTCA TAGAGAACAT CTACCTGCCT GCTGCACAGA CCATGAACTC TGGAACTTTT
    AACACCACTG TAGACATCAA ACTCAAGCAA TGGACTGATA AACAACTTCC TCATAAAGCA
    CTAGAGGTTG CTTGGGAGAC TTTGCAGGAA GAGTTTGCCC GCTTCATGGC CGAATACAAA
    GGGAAAGACC AGGATGACAT TTTTGACAAG CTGAAGGAAG CTGTCAAGGA CGAGAGCATC
    AAACGACACA AGTGGAATGA AAGAGCCATG GACAGCTTGA GAGTGATTCA ACACAACGCT
    CTGGAGGATC GTTCAATCAC AGACAAGCCC CAGTGGGATG CCGCTATACA GTTCATGGAG
    GAAACGCTGC AGTCTCGTCT TAAAGACACT GAGTCAGTAA TAGCTGATAT GGTGGGGCCA
    GACTGGAAGC AGAGGTGGAT GAGCTGGAAA AACCGAACAC CAGAGCAGCA CACCCGGAAT
    GAAACCAAAA ATGAGCTGGA GCGTCTGCTG AAACTGCATG AGGATCACAC AGCATATTTG
    GCCAATGATG AGGTCACTAC AGTGCGCAAA AACCTGGAGG CCCGAGGGGT GGAGGTGGAC
    CCTGTATTGA TTAAGGACAC ATGGCATCAG CTCTTTCGAC GTCACTTCCT ACAGAAGGCA
    CTACTACATT GCAACCTCTG CAGACGAGGT TTCTACTACT ATCAGAGGCA CTTCGTAGAC
    TCTGAGCTTG AGTGCAATGA TGTGGTTCTG TTCTGGAGGA TTCAGAGGAT GTTGGGCATC
    ACTGCCAATA CTCTTCGACA ACAGCTCACT AACACTGAGG TACGGCGTTT GGAGAAGAAC
    GTTAAAGAAG TATTAGAAGA CTTTGGAGAG GACAATGAGA AAAAAGTCCA GCTGATCACA
    GGCAGGAGGG TTCAACTGGC AGAAGACCTC AAAAAAGTTC GCGAAATTCA GGAGAAACTT
    GAAGCCTTCA TTGAAGCTCT TCACAAAGAG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332525.1
    CDS134..2869
    Translation

    Target ORF information:

    RefSeq Version XM_021476850.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCTCCCTG ATATGAGTGA ATACACATGG ATTGTGCCAG ACTTTGTTTG GGAGCTGAGC 
    GAAAATATAG ATTTAGACAA GCTAGCCAGT GCTTTACCTG AATTAGAAGA GATAGCCAAA
    CTACTACCTG ATATGGAGAA GATCGGAGAG AATTTCACCT TCCTGAAGAG CCTCCTCTCC
    AGCGGTTTGG GTAGTGAAGT CAATGGAGCT TCTGGTCTGC GTCTGTTATT AGAAACTACT
    GGCGAATCGG CCCTAAGAGC ACCAGACGTC CCACCAGCAT CTGCAGCCAT GGCTGACAGC
    GGAGACAAGC AGTTCAAGAA GCAGGGGCTG CTCAGCGAAC TCATTGTCAT TCAGCAGCAG
    ATCCAGCAGC ATGAGGAGGA GATGCGGCGC GCGGCAGCCA ATAACGCTCC GCCTCCGCCC
    CGTGACCCCA GTCCCAGCCC GGCCCCGAAC CCCAGCCCCT CGCAGTCCAA ACGCAAGTCA
    TCCGATAAAG AGAAAGTTGA CCAGCTTCAG GAGGAACTTC TTCGAACTCA GTTGAAGTAC
    CAACGGATGC TTGAGCGCTT GGAAAAGGAA AATAAAGAAT TGAGGAAAGT TGTATTGCAG
    AAGGATGACA AGGGTATTCA CCAAAGAAAA GTCAAGAAAT CTTTGATTGA TATGTACTCT
    GAGGTCCTGG ACATTCTTTC AGATTATGAC TCCAACTACA ACACTCAAGA TCACCTGCCA
    AGGGTGGTGG TAGTTGGGGA CCAGAGTGCG GGAAAGACTA GTGTGCTGGA GATGATTGCT
    CAAGCTAGGA TCTTTCCGAG AGGCTCTGGA GAAATGATGA CAAGGTCTCC TGTCAAGGTG
    ACTTTGAGTG AAGGCCCTCA CCATGTAGCA ATGTTTAAAG ACAGCTCTCG GGAGTTTGAC
    CTTGGCAAAG AGGAAGATCT TGCAGCACTG AGGCATGAGA TTGAGCTCAG GATGAGGAAG
    AGTGTGAAGG AAGGTCAGAC AGTCAGCCCT GAGACCATCT CTTTAAGTGT TAAGGGCCCT
    GGAATCCAGC GAATGGTGCT TGTAGATTTA CCAGGGGTCA TCAGTACTGT GACAACTGGA
    ATGGCCGCAG ACACAAAGGA AACCATTTTT AGCATTAGTA AAGCTTACAT GCAGAATCCA
    AACGCCATCA TCCTCTGTAT ACAGGATGGC TCAGTAGATG CAGAGCGCAG TATAGTCACT
    GATCTGGTCA GTCAAATGGA CCCTCAGGGC AAAAGGACCA TATTTGTTCT TACTAAGGTT
    GATCTGGCAG AAAAGAACCT GGCTAGTCCA AGCAGAATCC AGCAAATAGT AGAAGGCAAA
    TTATTCCCAA TGAAGGCTTT GGGCTACTTT GCTGTAGTAA CTGGCAAAGG CAGCCCTAAT
    GAGAGCATAG ATTCCATTAA AGACTATGAG GAGGACTTCT TCCAGAACTC AAGACTGCTG
    AAGGATGGCA TGTTGAAAGC TCATCAGGTG ACTACAAAGA ACCTGAGTCT TGCTGTGTCA
    GACTGTTTCT GGAAAATGGT CAGAGAGTCA GTAGAGCAAC AAGCAGATGC CTTTAAAGCT
    TCTCGATTTA ACCTGGAGAC TGAGTGGAAG AACAACTATC CTCGCTTACG AGAGCTGGAC
    AGGAACGAGC TCTACGAGAA GGCCAAAAAT GAGATCTTGG ATGAGGTGAT CAGCTTGAGT
    CAAGTTACAC CAAAACACTG GGAGTCTATC TTACAGAAGA AGCTTTGGGA AAGAGTCTCG
    ACACACGTCA TAGAGAACAT CTACCTGCCT GCTGCACAGA CCATGAACTC TGGAACTTTT
    AACACCACTG TAGACATCAA ACTCAAGCAA TGGACTGATA AACAACTTCC TCATAAAGCA
    CTAGAGGTTG CTTGGGAGAC TTTGCAGGAA GAGTTTGCCC GCTTCATGGC CGAATACAAA
    GGGAAAGACC AGGATGACAT TTTTGACAAG CTGAAGGAAG CTGTCAAGGA CGAGAGCATC
    AAACGACACA AGTGGAATGA AAGAGCCATG GACAGCTTGA GAGTGATTCA ACACAACGCT
    CTGGAGGATC GTTCAATCAC AGACAAGCCC CAGTGGGATG CCGCTATACA GTTCATGGAG
    GAAACGCTGC AGTCTCGTCT TAAAGACACT GAGTCAGTAA TAGCTGATAT GGTGGGGCCA
    GACTGGAAGC AGAGGTGGAT GAGCTGGAAA AACCGAACAC CAGAGCAGCA CACCCGGAAT
    GAAACCAAAA ATGAGCTGGA GCGTCTGCTG AAACTGCATG AGGATCACAC AGCATATTTG
    GCCAATGATG AGGTCACTAC AGTGCGCAAA AACCTGGAGG CCCGAGGGGT GGAGGTGGAC
    CCTGTATTGA TTAAGGACAC ATGGCATCAG CTCTTTCGAC GTCACTTCCT ACAGAAGGCA
    CTACTACATT GCAACCTCTG CAGACGAGGT TTCTACTACT ATCAGAGGCA CTTCGTAGAC
    TCTGAGCTTG AGTGCAATGA TGTGGTTCTG TTCTGGAGGA TTCAGAGGAT GTTGGGCATC
    ACTGCCAATA CTCTTCGACA ACAGCTCACT AACACTGAGG TACGGCGTTT GGAGAAGAAC
    GTTAAAGAAG TATTAGAAGA CTTTGGAGAG GACAATGAGA AAAAAGTCCA GCTGATCACA
    GGCAGGAGGG TTCAACTGGC AGAAGACCTC AAAAAAGTTC GCGAAATTCA GGAGAAACTT
    GAAGCCTTCA TTGAAGCTCT TCACAAAGAG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59356
    Clone ID Related Accession (Same CDS sequence) XM_017356479.1
    Accession Version XM_017356479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGTCATC CGATAAAGAG
    AAAGTTGACC AGCTTCAGGA GGAACTTCTT CGAACTCAGT TGAAGTACCA ACGGATGCTT
    GAGCGCTTGG AAAAGGAAAA TAAAGAATTG AGGAAAGTTG TATTGCAGAA GGATGACAAG
    GGTATTCACC AAAGAAAAGT CAAGAAATCT TTGATTGATA TGTACTCTGA GGTCCTGGAC
    ATTCTTTCAG ATTATGACTC CAACTACAAC ACTCAAGATC ACCTGCCAAG GGTGGTGGTA
    GTTGGGGACC AGAGTGCGGG AAAGACTAGT GTGCTGGAGA TGATTGCTCA AGCTAGGATC
    TTTCCGAGAG GCTCTGGAGA AATGATGACA AGGTCTCCTG TCAAGGTGAC TTTGAGTGAA
    GGCCCTCACC ATGTAGCAAT GTTTAAAGAC AGCTCTCGGG AGTTTGACCT TGGCAAAGAG
    GAAGATCTTG CAGCACTGAG GCATGAGATT GAGCTCAGGA TGAGGAAGAG TGTGAAGGAA
    GGTCAGACAG TCAGCCCTGA GACCATCTCT TTAAGTGTTA AGGGCCCTGG AATCCAGCGA
    ATGGTGCTTG TAGATTTACC AGGGGTCATC AGTACTGTGA CAACTGGAAT GGCCGCAGAC
    ACAAAGGAAA CCATTTTTAG CATTAGTAAA GCTTACATGC AGAATCCAAA CGCCATCATC
    CTCTGTATAC AGGATGGCTC AGTAGATGCA GAGCGCAGTA TAGTCACTGA TCTGGTCAGT
    CAAATGGACC CTCAGGGCAA AAGGACCATA TTTGTTCTTA CTAAGGTTGA TCTGGCAGAA
    AAGAACCTGG CTAGTCCAAG CAGAATCCAG CAAATAGTAG AAGGCAAATT ATTCCCAATG
    AAGGCTTTGG GCTACTTTGC TGTAGTAACT GGCAAAGGCA GCCCTAATGA GAGCATAGAT
    TCCATTAAAG ACTATGAGGA GGACTTCTTC CAGAACTCAA GACTGCTGAA GGATGGCATG
    TTGAAAGCTC ATCAGGTGAC TACAAAGAAC CTGAGTCTTG CTGTGTCAGA CTGTTTCTGG
    AAAATGGTCA GAGAGTCAGT AGAGCAACAA GCAGATGCCT TTAAAGCTTC TCGATTTAAC
    CTGGAGACTG AGTGGAAGAA CAACTATCCT CGCTTACGAG AGCTGGACAG GAACGAGCTC
    TACGAGAAGG CCAAAAATGA GATCTTGGAT GAGGTGATCA GCTTGAGTCA AGTTACACCA
    AAACACTGGG AGTCTATCTT ACAGAAGAAG CTTTGGGAAA GAGTCTCGAC ACACGTCATA
    GAGAACATCT ACCTGCCTGC TGCACAGACC ATGAACTCTG GAACTTTTAA CACCACTGTA
    GACATCAAAC TCAAGCAATG GACTGATAAA CAACTTCCTC ATAAAGCACT AGAGGTTGCT
    TGGGAGACTT TGCAGGAAGA GTTTGCCCGC TTCATGGCCG AATACAAAGG GAAAGACCAG
    GATGACATTT TTGACAAGCT GAAGGAAGCT GTCAAGGACG AGAGCATCAA ACGACACAAG
    TGGAATGAAA GAGCCATGGA CAGCTTGAGA GTGATTCAAC ACAACGCTCT GGAGGATCGT
    TCAATCACAG ACAAGCCCCA GTGGGATGCC GCTATACAGT TCATGGAGGA AACGCTGCAG
    TCTCGTCTTA AAGACACTGA GTCAGTAATA GCTGATATGG TGGGGCCAGA CTGGAAGCAG
    AGGTGGATGA GCTGGAAAAA CCGAACACCA GAGCAGCACA CCCGGAATGA AACCAAAAAT
    GAGCTGGAGC GTCTGCTGAA ACTGCATGAG GATCACACAG CATATTTGGC CAATGATGAG
    GTCACTACAG TGCGCAAAAA CCTGGAGGCC CGAGGGGTGG AGGTGGACCC TGTATTGATT
    AAGGACACAT GGCATCAGCT CTTTCGACGT CACTTCCTAC AGAAGGCACT ACTACATTGC
    AACCTCTGCA GACGAGGTTT CTACTACTAT CAGAGGCACT TCGTAGACTC TGAGCTTGAG
    TGCAATGATG TGGTTCTGTT CTGGAGGATT CAGAGGATGT TGGGCATCAC TGCCAATACT
    CTTCGACAAC AGCTCACTAA CACTGAGGTA CGGCGTTTGG AGAAGAACGT TAAAGAAGTA
    TTAGAAGACT TTGGAGAGGA CAATGAGAAA AAAGTCCAGC TGATCACAGG CAGGAGGGTT
    CAACTGGCAG AAGACCTCAA AAAAGTTCGC GAAATTCAGG AGAAACTTGA AGCCTTCATT
    GAAGCTCTTC ACAAAGAGAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211968.1
    CDS40..3003
    Translation

    Target ORF information:

    RefSeq Version XM_017356479.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGTCATC CGATAAAGAG
    AAAGTTGACC AGCTTCAGGA GGAACTTCTT CGAACTCAGT TGAAGTACCA ACGGATGCTT
    GAGCGCTTGG AAAAGGAAAA TAAAGAATTG AGGAAAGTTG TATTGCAGAA GGATGACAAG
    GGTATTCACC AAAGAAAAGT CAAGAAATCT TTGATTGATA TGTACTCTGA GGTCCTGGAC
    ATTCTTTCAG ATTATGACTC CAACTACAAC ACTCAAGATC ACCTGCCAAG GGTGGTGGTA
    GTTGGGGACC AGAGTGCGGG AAAGACTAGT GTGCTGGAGA TGATTGCTCA AGCTAGGATC
    TTTCCGAGAG GCTCTGGAGA AATGATGACA AGGTCTCCTG TCAAGGTGAC TTTGAGTGAA
    GGCCCTCACC ATGTAGCAAT GTTTAAAGAC AGCTCTCGGG AGTTTGACCT TGGCAAAGAG
    GAAGATCTTG CAGCACTGAG GCATGAGATT GAGCTCAGGA TGAGGAAGAG TGTGAAGGAA
    GGTCAGACAG TCAGCCCTGA GACCATCTCT TTAAGTGTTA AGGGCCCTGG AATCCAGCGA
    ATGGTGCTTG TAGATTTACC AGGGGTCATC AGTACTGTGA CAACTGGAAT GGCCGCAGAC
    ACAAAGGAAA CCATTTTTAG CATTAGTAAA GCTTACATGC AGAATCCAAA CGCCATCATC
    CTCTGTATAC AGGATGGCTC AGTAGATGCA GAGCGCAGTA TAGTCACTGA TCTGGTCAGT
    CAAATGGACC CTCAGGGCAA AAGGACCATA TTTGTTCTTA CTAAGGTTGA TCTGGCAGAA
    AAGAACCTGG CTAGTCCAAG CAGAATCCAG CAAATAGTAG AAGGCAAATT ATTCCCAATG
    AAGGCTTTGG GCTACTTTGC TGTAGTAACT GGCAAAGGCA GCCCTAATGA GAGCATAGAT
    TCCATTAAAG ACTATGAGGA GGACTTCTTC CAGAACTCAA GACTGCTGAA GGATGGCATG
    TTGAAAGCTC ATCAGGTGAC TACAAAGAAC CTGAGTCTTG CTGTGTCAGA CTGTTTCTGG
    AAAATGGTCA GAGAGTCAGT AGAGCAACAA GCAGATGCCT TTAAAGCTTC TCGATTTAAC
    CTGGAGACTG AGTGGAAGAA CAACTATCCT CGCTTACGAG AGCTGGACAG GAACGAGCTC
    TACGAGAAGG CCAAAAATGA GATCTTGGAT GAGGTGATCA GCTTGAGTCA AGTTACACCA
    AAACACTGGG AGTCTATCTT ACAGAAGAAG CTTTGGGAAA GAGTCTCGAC ACACGTCATA
    GAGAACATCT ACCTGCCTGC TGCACAGACC ATGAACTCTG GAACTTTTAA CACCACTGTA
    GACATCAAAC TCAAGCAATG GACTGATAAA CAACTTCCTC ATAAAGCACT AGAGGTTGCT
    TGGGAGACTT TGCAGGAAGA GTTTGCCCGC TTCATGGCCG AATACAAAGG GAAAGACCAG
    GATGACATTT TTGACAAGCT GAAGGAAGCT GTCAAGGACG AGAGCATCAA ACGACACAAG
    TGGAATGAAA GAGCCATGGA CAGCTTGAGA GTGATTCAAC ACAACGCTCT GGAGGATCGT
    TCAATCACAG ACAAGCCCCA GTGGGATGCC GCTATACAGT TCATGGAGGA AACGCTGCAG
    TCTCGTCTTA AAGACACTGA GTCAGTAATA GCTGATATGG TGGGGCCAGA CTGGAAGCAG
    AGGTGGATGA GCTGGAAAAA CCGAACACCA GAGCAGCACA CCCGGAATGA AACCAAAAAT
    GAGCTGGAGC GTCTGCTGAA ACTGCATGAG GATCACACAG CATATTTGGC CAATGATGAG
    GTCACTACAG TGCGCAAAAA CCTGGAGGCC CGAGGGGTGG AGGTGGACCC TGTATTGATT
    AAGGACACAT GGCATCAGCT CTTTCGACGT CACTTCCTAC AGAAGGCACT ACTACATTGC
    AACCTCTGCA GACGAGGTTT CTACTACTAT CAGAGGCACT TCGTAGACTC TGAGCTTGAG
    TGCAATGATG TGGTTCTGTT CTGGAGGATT CAGAGGATGT TGGGCATCAC TGCCAATACT
    CTTCGACAAC AGCTCACTAA CACTGAGGTA CGGCGTTTGG AGAAGAACGT TAAAGAAGTA
    TTAGAAGACT TTGGAGAGGA CAATGAGAAA AAAGTCCAGC TGATCACAGG CAGGAGGGTT
    CAACTGGCAG AAGACCTCAA AAAAGTTCGC GAAATTCAGG AGAAACTTGA AGCCTTCATT
    GAAGCTCTTC ACAAAGAGAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59357
    Clone ID Related Accession (Same CDS sequence) XM_017356480.1
    Accession Version XM_017356480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGAA ACTACTGGCG AATCGGCCCT AAGAGCACCA
    GACGTCCCAC CAGCATCTGC AGCCATGGCT GACAGCGGAG ACAAGCAGTT CAAGAAGTCA
    TCCGATAAAG AGAAAGTTGA CCAGCTTCAG GAGGAACTTC TTCGAACTCA GTTGAAGTAC
    CAACGGATGC TTGAGCGCTT GGAAAAGGAA AATAAAGAAT TGAGGAAAGT TGTATTGCAG
    AAGGATGACA AGGGTATTCA CCAAAGAAAA GTCAAGAAAT CTTTGATTGA TATGTACTCT
    GAGGTCCTGG ACATTCTTTC AGATTATGAC TCCAACTACA ACACTCAAGA TCACCTGCCA
    AGGGTGGTGG TAGTTGGGGA CCAGAGTGCG GGAAAGACTA GTGTGCTGGA GATGATTGCT
    CAAGCTAGGA TCTTTCCGAG AGGCTCTGGA GAAATGATGA CAAGGTCTCC TGTCAAGGTG
    ACTTTGAGTG AAGGCCCTCA CCATGTAGCA ATGTTTAAAG ACAGCTCTCG GGAGTTTGAC
    CTTGGCAAAG AGGAAGATCT TGCAGCACTG AGGCATGAGA TTGAGCTCAG GATGAGGAAG
    AGTGTGAAGG AAGGTCAGAC AGTCAGCCCT GAGACCATCT CTTTAAGTGT TAAGGGCCCT
    GGAATCCAGC GAATGGTGCT TGTAGATTTA CCAGGGGTCA TCAGTACTGT GACAACTGGA
    ATGGCCGCAG ACACAAAGGA AACCATTTTT AGCATTAGTA AAGCTTACAT GCAGAATCCA
    AACGCCATCA TCCTCTGTAT ACAGGATGGC TCAGTAGATG CAGAGCGCAG TATAGTCACT
    GATCTGGTCA GTCAAATGGA CCCTCAGGGC AAAAGGACCA TATTTGTTCT TACTAAGGTT
    GATCTGGCAG AAAAGAACCT GGCTAGTCCA AGCAGAATCC AGCAAATAGT AGAAGGCAAA
    TTATTCCCAA TGAAGGCTTT GGGCTACTTT GCTGTAGTAA CTGGCAAAGG CAGCCCTAAT
    GAGAGCATAG ATTCCATTAA AGACTATGAG GAGGACTTCT TCCAGAACTC AAGACTGCTG
    AAGGATGGCA TGTTGAAAGC TCATCAGGTG ACTACAAAGA ACCTGAGTCT TGCTGTGTCA
    GACTGTTTCT GGAAAATGGT CAGAGAGTCA GTAGAGCAAC AAGCAGATGC CTTTAAAGCT
    TCTCGATTTA ACCTGGAGAC TGAGTGGAAG AACAACTATC CTCGCTTACG AGAGCTGGAC
    AGGAACGAGC TCTACGAGAA GGCCAAAAAT GAGATCTTGG ATGAGGTGAT CAGCTTGAGT
    CAAGTTACAC CAAAACACTG GGAGTCTATC TTACAGAAGA AGCTTTGGGA AAGAGTCTCG
    ACACACGTCA TAGAGAACAT CTACCTGCCT GCTGCACAGA CCATGAACTC TGGAACTTTT
    AACACCACTG TAGACATCAA ACTCAAGCAA TGGACTGATA AACAACTTCC TCATAAAGCA
    CTAGAGGTTG CTTGGGAGAC TTTGCAGGAA GAGTTTGCCC GCTTCATGGC CGAATACAAA
    GGGAAAGACC AGGATGACAT TTTTGACAAG CTGAAGGAAG CTGTCAAGGA CGAGAGCATC
    AAACGACACA AGTGGAATGA AAGAGCCATG GACAGCTTGA GAGTGATTCA ACACAACGCT
    CTGGAGGATC GTTCAATCAC AGACAAGCCC CAGTGGGATG CCGCTATACA GTTCATGGAG
    GAAACGCTGC AGTCTCGTCT TAAAGACACT GAGTCAGTAA TAGCTGATAT GGTGGGGCCA
    GACTGGAAGC AGAGGTGGAT GAGCTGGAAA AACCGAACAC CAGAGCAGCA CACCCGGAAT
    GAAACCAAAA ATGAGCTGGA GCGTCTGCTG AAACTGCATG AGGATCACAC AGCATATTTG
    GCCAATGATG AGGTCACTAC AGTGCGCAAA AACCTGGAGG CCCGAGGGGT GGAGGTGGAC
    CCTGTATTGA TTAAGGACAC ATGGCATCAG CTCTTTCGAC GTCACTTCCT ACAGAAGGCA
    CTACTACATT GCAACCTCTG CAGACGAGGT TTCTACTACT ATCAGAGGCA CTTCGTAGAC
    TCTGAGCTTG AGTGCAATGA TGTGGTTCTG TTCTGGAGGA TTCAGAGGAT GTTGGGCATC
    ACTGCCAATA CTCTTCGACA ACAGCTCACT AACACTGAGG TACGGCGTTT GGAGAAGAAC
    GTTAAAGAAG TATTAGAAGA CTTTGGAGAG GACAATGAGA AAAAAGTCCA GCTGATCACA
    GGCAGGAGGG TTCAACTGGC AGAAGACCTC AAAAAAGTTC GCGAAATTCA GGAGAAACTT
    GAAGCCTTCA TTGAAGCTCT TCACAAAGAG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211969.1
    CDS40..2955
    Translation

    Target ORF information:

    RefSeq Version XM_017356480.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGAA ACTACTGGCG AATCGGCCCT AAGAGCACCA
    GACGTCCCAC CAGCATCTGC AGCCATGGCT GACAGCGGAG ACAAGCAGTT CAAGAAGTCA
    TCCGATAAAG AGAAAGTTGA CCAGCTTCAG GAGGAACTTC TTCGAACTCA GTTGAAGTAC
    CAACGGATGC TTGAGCGCTT GGAAAAGGAA AATAAAGAAT TGAGGAAAGT TGTATTGCAG
    AAGGATGACA AGGGTATTCA CCAAAGAAAA GTCAAGAAAT CTTTGATTGA TATGTACTCT
    GAGGTCCTGG ACATTCTTTC AGATTATGAC TCCAACTACA ACACTCAAGA TCACCTGCCA
    AGGGTGGTGG TAGTTGGGGA CCAGAGTGCG GGAAAGACTA GTGTGCTGGA GATGATTGCT
    CAAGCTAGGA TCTTTCCGAG AGGCTCTGGA GAAATGATGA CAAGGTCTCC TGTCAAGGTG
    ACTTTGAGTG AAGGCCCTCA CCATGTAGCA ATGTTTAAAG ACAGCTCTCG GGAGTTTGAC
    CTTGGCAAAG AGGAAGATCT TGCAGCACTG AGGCATGAGA TTGAGCTCAG GATGAGGAAG
    AGTGTGAAGG AAGGTCAGAC AGTCAGCCCT GAGACCATCT CTTTAAGTGT TAAGGGCCCT
    GGAATCCAGC GAATGGTGCT TGTAGATTTA CCAGGGGTCA TCAGTACTGT GACAACTGGA
    ATGGCCGCAG ACACAAAGGA AACCATTTTT AGCATTAGTA AAGCTTACAT GCAGAATCCA
    AACGCCATCA TCCTCTGTAT ACAGGATGGC TCAGTAGATG CAGAGCGCAG TATAGTCACT
    GATCTGGTCA GTCAAATGGA CCCTCAGGGC AAAAGGACCA TATTTGTTCT TACTAAGGTT
    GATCTGGCAG AAAAGAACCT GGCTAGTCCA AGCAGAATCC AGCAAATAGT AGAAGGCAAA
    TTATTCCCAA TGAAGGCTTT GGGCTACTTT GCTGTAGTAA CTGGCAAAGG CAGCCCTAAT
    GAGAGCATAG ATTCCATTAA AGACTATGAG GAGGACTTCT TCCAGAACTC AAGACTGCTG
    AAGGATGGCA TGTTGAAAGC TCATCAGGTG ACTACAAAGA ACCTGAGTCT TGCTGTGTCA
    GACTGTTTCT GGAAAATGGT CAGAGAGTCA GTAGAGCAAC AAGCAGATGC CTTTAAAGCT
    TCTCGATTTA ACCTGGAGAC TGAGTGGAAG AACAACTATC CTCGCTTACG AGAGCTGGAC
    AGGAACGAGC TCTACGAGAA GGCCAAAAAT GAGATCTTGG ATGAGGTGAT CAGCTTGAGT
    CAAGTTACAC CAAAACACTG GGAGTCTATC TTACAGAAGA AGCTTTGGGA AAGAGTCTCG
    ACACACGTCA TAGAGAACAT CTACCTGCCT GCTGCACAGA CCATGAACTC TGGAACTTTT
    AACACCACTG TAGACATCAA ACTCAAGCAA TGGACTGATA AACAACTTCC TCATAAAGCA
    CTAGAGGTTG CTTGGGAGAC TTTGCAGGAA GAGTTTGCCC GCTTCATGGC CGAATACAAA
    GGGAAAGACC AGGATGACAT TTTTGACAAG CTGAAGGAAG CTGTCAAGGA CGAGAGCATC
    AAACGACACA AGTGGAATGA AAGAGCCATG GACAGCTTGA GAGTGATTCA ACACAACGCT
    CTGGAGGATC GTTCAATCAC AGACAAGCCC CAGTGGGATG CCGCTATACA GTTCATGGAG
    GAAACGCTGC AGTCTCGTCT TAAAGACACT GAGTCAGTAA TAGCTGATAT GGTGGGGCCA
    GACTGGAAGC AGAGGTGGAT GAGCTGGAAA AACCGAACAC CAGAGCAGCA CACCCGGAAT
    GAAACCAAAA ATGAGCTGGA GCGTCTGCTG AAACTGCATG AGGATCACAC AGCATATTTG
    GCCAATGATG AGGTCACTAC AGTGCGCAAA AACCTGGAGG CCCGAGGGGT GGAGGTGGAC
    CCTGTATTGA TTAAGGACAC ATGGCATCAG CTCTTTCGAC GTCACTTCCT ACAGAAGGCA
    CTACTACATT GCAACCTCTG CAGACGAGGT TTCTACTACT ATCAGAGGCA CTTCGTAGAC
    TCTGAGCTTG AGTGCAATGA TGTGGTTCTG TTCTGGAGGA TTCAGAGGAT GTTGGGCATC
    ACTGCCAATA CTCTTCGACA ACAGCTCACT AACACTGAGG TACGGCGTTT GGAGAAGAAC
    GTTAAAGAAG TATTAGAAGA CTTTGGAGAG GACAATGAGA AAAAAGTCCA GCTGATCACA
    GGCAGGAGGG TTCAACTGGC AGAAGACCTC AAAAAAGTTC GCGAAATTCA GGAGAAACTT
    GAAGCCTTCA TTGAAGCTCT TCACAAAGAG AAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12003
    Clone ID Related Accession (Same CDS sequence) XM_017356477.2
    Accession Version XM_017356477.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3102bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356477.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG GGGCTGCTCA GCGAACTCAT TGTCATTCAG
    CAGCAGATCC AGCAGCATGA GGAGGAGATG CGGCGCGCGG CAGCCAATAA CGCTCCGCCT
    CCGCCCCGTG ACCCCAGTCC CAGCCCGGCC CCGAACCCCA GCCCCTCGCA GTCCAAACGC
    AAGTCATCCG ATAAAGAGAA AGTTGACCAG CTTCAGGAGG AACTTCTTCG AACTCAGTTG
    AAGTACCAAC GGATGCTTGA GCGCTTGGAA AAGGAAAATA AAGAATTGAG GAAAGTTGTA
    TTGCAGAAGG ATGACAAGGG TATTCACCAA AGAAAAGTCA AGAAATCTTT GATTGATATG
    TACTCTGAGG TCCTGGACAT TCTTTCAGAT TATGACTCCA ACTACAACAC TCAAGATCAC
    CTGCCAAGGG TGGTGGTAGT TGGGGACCAG AGTGCGGGAA AGACTAGTGT GCTGGAGATG
    ATTGCTCAAG CTAGGATCTT TCCGAGAGGC TCTGGAGAAA TGATGACAAG GTCTCCTGTC
    AAGGTGACTT TGAGTGAAGG CCCTCACCAT GTAGCAATGT TTAAAGACAG CTCTCGGGAG
    TTTGACCTTG GCAAAGAGGA AGATCTTGCA GCACTGAGGC ATGAGATTGA GCTCAGGATG
    AGGAAGAGTG TGAAGGAAGG TCAGACAGTC AGCCCTGAGA CCATCTCTTT AAGTGTTAAG
    GGCCCTGGAA TCCAGCGAAT GGTGCTTGTA GATTTACCAG GGGTCATCAG TACTGTGACA
    ACTGGAATGG CCGCAGACAC AAAGGAAACC ATTTTTAGCA TTAGTAAAGC TTACATGCAG
    AATCCAAACG CCATCATCCT CTGTATACAG GATGGCTCAG TAGATGCAGA GCGCAGTATA
    GTCACTGATC TGGTCAGTCA AATGGACCCT CAGGGCAAAA GGACCATATT TGTTCTTACT
    AAGGTTGATC TGGCAGAAAA GAACCTGGCT AGTCCAAGCA GAATCCAGCA AATAGTAGAA
    GGCAAATTAT TCCCAATGAA GGCTTTGGGC TACTTTGCTG TAGTAACTGG CAAAGGCAGC
    CCTAATGAGA GCATAGATTC CATTAAAGAC TATGAGGAGG ACTTCTTCCA GAACTCAAGA
    CTGCTGAAGG ATGGCATGTT GAAAGCTCAT CAGGTGACTA CAAAGAACCT GAGTCTTGCT
    GTGTCAGACT GTTTCTGGAA AATGGTCAGA GAGTCAGTAG AGCAACAAGC AGATGCCTTT
    AAAGCTTCTC GATTTAACCT GGAGACTGAG TGGAAGAACA ACTATCCTCG CTTACGAGAG
    CTGGACAGGA ACGAGCTCTA CGAGAAGGCC AAAAATGAGA TCTTGGATGA GGTGATCAGC
    TTGAGTCAAG TTACACCAAA ACACTGGGAG TCTATCTTAC AGAAGAAGCT TTGGGAAAGA
    GTCTCGACAC ACGTCATAGA GAACATCTAC CTGCCTGCTG CACAGACCAT GAACTCTGGA
    ACTTTTAACA CCACTGTAGA CATCAAACTC AAGCAATGGA CTGATAAACA ACTTCCTCAT
    AAAGCACTAG AGGTTGCTTG GGAGACTTTG CAGGAAGAGT TTGCCCGCTT CATGGCCGAA
    TACAAAGGGA AAGACCAGGA TGACATTTTT GACAAGCTGA AGGAAGCTGT CAAGGACGAG
    AGCATCAAAC GACACAAGTG GAATGAAAGA GCCATGGACA GCTTGAGAGT GATTCAACAC
    AACGCTCTGG AGGATCGTTC AATCACAGAC AAGCCCCAGT GGGATGCCGC TATACAGTTC
    ATGGAGGAAA CGCTGCAGTC TCGTCTTAAA GACACTGAGT CAGTAATAGC TGATATGGTG
    GGGCCAGACT GGAAGCAGAG GTGGATGAGC TGGAAAAACC GAACACCAGA GCAGCACACC
    CGGAATGAAA CCAAAAATGA GCTGGAGCGT CTGCTGAAAC TGCATGAGGA TCACACAGCA
    TATTTGGCCA ATGATGAGGT CACTACAGTG CGCAAAAACC TGGAGGCCCG AGGGGTGGAG
    GTGGACCCTG TATTGATTAA GGACACATGG CATCAGCTCT TTCGACGTCA CTTCCTACAG
    AAGGCACTAC TACATTGCAA CCTCTGCAGA CGAGGTTTCT ACTACTATCA GAGGCACTTC
    GTAGACTCTG AGCTTGAGTG CAATGATGTG GTTCTGTTCT GGAGGATTCA GAGGATGTTG
    GGCATCACTG CCAATACTCT TCGACAACAG CTCACTAACA CTGAGGTACG GCGTTTGGAG
    AAGAACGTTA AAGAAGTATT AGAAGACTTT GGAGAGGACA ATGAGAAAAA AGTCCAGCTG
    ATCACAGGCA GGAGGGTTCA ACTGGCAGAA GACCTCAAAA AAGTTCGCGA AATTCAGGAG
    AAACTTGAAG CCTTCATTGA AGCTCTTCAC AAAGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211966.2
    CDS174..3275
    Translation

    Target ORF information:

    RefSeq Version XM_017356477.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356477.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG GGGCTGCTCA GCGAACTCAT TGTCATTCAG
    CAGCAGATCC AGCAGCATGA GGAGGAGATG CGGCGCGCGG CAGCCAATAA CGCTCCGCCT
    CCGCCCCGTG ACCCCAGTCC CAGCCCGGCC CCGAACCCCA GCCCCTCGCA GTCCAAACGC
    AAGTCATCCG ATAAAGAGAA AGTTGACCAG CTTCAGGAGG AACTTCTTCG AACTCAGTTG
    AAGTACCAAC GGATGCTTGA GCGCTTGGAA AAGGAAAATA AAGAATTGAG GAAAGTTGTA
    TTGCAGAAGG ATGACAAGGG TATTCACCAA AGAAAAGTCA AGAAATCTTT GATTGATATG
    TACTCTGAGG TCCTGGACAT TCTTTCAGAT TATGACTCCA ACTACAACAC TCAAGATCAC
    CTGCCAAGGG TGGTGGTAGT TGGGGACCAG AGTGCGGGAA AGACTAGTGT GCTGGAGATG
    ATTGCTCAAG CTAGGATCTT TCCGAGAGGC TCTGGAGAAA TGATGACAAG GTCTCCTGTC
    AAGGTGACTT TGAGTGAAGG CCCTCACCAT GTAGCAATGT TTAAAGACAG CTCTCGGGAG
    TTTGACCTTG GCAAAGAGGA AGATCTTGCA GCACTGAGGC ATGAGATTGA GCTCAGGATG
    AGGAAGAGTG TGAAGGAAGG TCAGACAGTC AGCCCTGAGA CCATCTCTTT AAGTGTTAAG
    GGCCCTGGAA TCCAGCGAAT GGTGCTTGTA GATTTACCAG GGGTCATCAG TACTGTGACA
    ACTGGAATGG CCGCAGACAC AAAGGAAACC ATTTTTAGCA TTAGTAAAGC TTACATGCAG
    AATCCAAACG CCATCATCCT CTGTATACAG GATGGCTCAG TAGATGCAGA GCGCAGTATA
    GTCACTGATC TGGTCAGTCA AATGGACCCT CAGGGCAAAA GGACCATATT TGTTCTTACT
    AAGGTTGATC TGGCAGAAAA GAACCTGGCT AGTCCAAGCA GAATCCAGCA AATAGTAGAA
    GGCAAATTAT TCCCAATGAA GGCTTTGGGC TACTTTGCTG TAGTAACTGG CAAAGGCAGC
    CCTAATGAGA GCATAGATTC CATTAAAGAC TATGAGGAGG ACTTCTTCCA GAACTCAAGA
    CTGCTGAAGG ATGGCATGTT GAAAGCTCAT CAGGTGACTA CAAAGAACCT GAGTCTTGCT
    GTGTCAGACT GTTTCTGGAA AATGGTCAGA GAGTCAGTAG AGCAACAAGC AGATGCCTTT
    AAAGCTTCTC GATTTAACCT GGAGACTGAG TGGAAGAACA ACTATCCTCG CTTACGAGAG
    CTGGACAGGA ACGAGCTCTA CGAGAAGGCC AAAAATGAGA TCTTGGATGA GGTGATCAGC
    TTGAGTCAAG TTACACCAAA ACACTGGGAG TCTATCTTAC AGAAGAAGCT TTGGGAAAGA
    GTCTCGACAC ACGTCATAGA GAACATCTAC CTGCCTGCTG CACAGACCAT GAACTCTGGA
    ACTTTTAACA CCACTGTAGA CATCAAACTC AAGCAATGGA CTGATAAACA ACTTCCTCAT
    AAAGCACTAG AGGTTGCTTG GGAGACTTTG CAGGAAGAGT TTGCCCGCTT CATGGCCGAA
    TACAAAGGGA AAGACCAGGA TGACATTTTT GACAAGCTGA AGGAAGCTGT CAAGGACGAG
    AGCATCAAAC GACACAAGTG GAATGAAAGA GCCATGGACA GCTTGAGAGT GATTCAACAC
    AACGCTCTGG AGGATCGTTC AATCACAGAC AAGCCCCAGT GGGATGCCGC TATACAGTTC
    ATGGAGGAAA CGCTGCAGTC TCGTCTTAAA GACACTGAGT CAGTAATAGC TGATATGGTG
    GGGCCAGACT GGAAGCAGAG GTGGATGAGC TGGAAAAACC GAACACCAGA GCAGCACACC
    CGGAATGAAA CCAAAAATGA GCTGGAGCGT CTGCTGAAAC TGCATGAGGA TCACACAGCA
    TATTTGGCCA ATGATGAGGT CACTACAGTG CGCAAAAACC TGGAGGCCCG AGGGGTGGAG
    GTGGACCCTG TATTGATTAA GGACACATGG CATCAGCTCT TTCGACGTCA CTTCCTACAG
    AAGGCACTAC TACATTGCAA CCTCTGCAGA CGAGGTTTCT ACTACTATCA GAGGCACTTC
    GTAGACTCTG AGCTTGAGTG CAATGATGTG GTTCTGTTCT GGAGGATTCA GAGGATGTTG
    GGCATCACTG CCAATACTCT TCGACAACAG CTCACTAACA CTGAGGTACG GCGTTTGGAG
    AAGAACGTTA AAGAAGTATT AGAAGACTTT GGAGAGGACA ATGAGAAAAA AGTCCAGCTG
    ATCACAGGCA GGAGGGTTCA ACTGGCAGAA GACCTCAAAA AAGTTCGCGA AATTCAGGAG
    AAACTTGAAG CCTTCATTGA AGCTCTTCAC AAAGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12004
    Clone ID Related Accession (Same CDS sequence) XM_021476847.1
    Accession Version XM_021476847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGCAGGGGCT GCTCAGCGAA
    CTCATTGTCA TTCAGCAGCA GATCCAGCAG CATGAGGAGG AGATGCGGCG CGCGGCAGCC
    AATAACGCTC CGCCTCCGCC CCGTGACCCC AGTCCCAGCC CGGCCCCGAA CCCCAGCCCC
    TCGCAGTCCA AACGCAAGTC ATCCGATAAA GAGAAAGTTG ACCAGCTTCA GGAGGAACTT
    CTTCGAACTC AGTTGAAGTA CCAACGGATG CTTGAGCGCT TGGAAAAGGA AAATAAAGAA
    TTGAGGAAAG TTGTATTGCA GAAGGATGAC AAGGGTATTC ACCAAAGAAA AGTCAAGAAA
    TCTTTGATTG ATATGTACTC TGAGGTCCTG GACATTCTTT CAGATTATGA CTCCAACTAC
    AACACTCAAG ATCACCTGCC AAGGGTGGTG GTAGTTGGGG ACCAGAGTGC GGGAAAGACT
    AGTGTGCTGG AGATGATTGC TCAAGCTAGG ATCTTTCCGA GAGGCTCTGG AGAAATGATG
    ACAAGGTCTC CTGTCAAGGT GACTTTGAGT GAAGGCCCTC ACCATGTAGC AATGTTTAAA
    GACAGCTCTC GGGAGTTTGA CCTTGGCAAA GAGGAAGATC TTGCAGCACT GAGGCATGAG
    ATTGAGCTCA GGATGAGGAA GAGTGTGAAG GAAGGTCAGA CAGTCAGCCC TGAGACCATC
    TCTTTAAGTG TTAAGGGCCC TGGAATCCAG CGAATGGTGC TTGTAGATTT ACCAGGGGTC
    ATCAGTACTG TGACAACTGG AATGGCCGCA GACACAAAGG AAACCATTTT TAGCATTAGT
    AAAGCTTACA TGCAGAATCC AAACGCCATC ATCCTCTGTA TACAGGATGG CTCAGTAGAT
    GCAGAGCGCA GTATAGTCAC TGATCTGGTC AGTCAAATGG ACCCTCAGGG CAAAAGGACC
    ATATTTGTTC TTACTAAGGT TGATCTGGCA GAAAAGAACC TGGCTAGTCC AAGCAGAATC
    CAGCAAATAG TAGAAGGCAA ATTATTCCCA ATGAAGGCTT TGGGCTACTT TGCTGTAGTA
    ACTGGCAAAG GCAGCCCTAA TGAGAGCATA GATTCCATTA AAGACTATGA GGAGGACTTC
    TTCCAGAACT CAAGACTGCT GAAGGATGGC ATGTTGAAAG CTCATCAGGT GACTACAAAG
    AACCTGAGTC TTGCTGTGTC AGACTGTTTC TGGAAAATGG TCAGAGAGTC AGTAGAGCAA
    CAAGCAGATG CCTTTAAAGC TTCTCGATTT AACCTGGAGA CTGAGTGGAA GAACAACTAT
    CCTCGCTTAC GAGAGCTGGA CAGGAACGAG CTCTACGAGA AGGCCAAAAA TGAGATCTTG
    GATGAGGTGA TCAGCTTGAG TCAAGTTACA CCAAAACACT GGGAGTCTAT CTTACAGAAG
    AAGCTTTGGG AAAGAGTCTC GACACACGTC ATAGAGAACA TCTACCTGCC TGCTGCACAG
    ACCATGAACT CTGGAACTTT TAACACCACT GTAGACATCA AACTCAAGCA ATGGACTGAT
    AAACAACTTC CTCATAAAGC ACTAGAGGTT GCTTGGGAGA CTTTGCAGGA AGAGTTTGCC
    CGCTTCATGG CCGAATACAA AGGGAAAGAC CAGGATGACA TTTTTGACAA GCTGAAGGAA
    GCTGTCAAGG ACGAGAGCAT CAAACGACAC AAGTGGAATG AAAGAGCCAT GGACAGCTTG
    AGAGTGATTC AACACAACGC TCTGGAGGAT CGTTCAATCA CAGACAAGCC CCAGTGGGAT
    GCCGCTATAC AGTTCATGGA GGAAACGCTG CAGTCTCGTC TTAAAGACAC TGAGTCAGTA
    ATAGCTGATA TGGTGGGGCC AGACTGGAAG CAGAGGTGGA TGAGCTGGAA AAACCGAACA
    CCAGAGCAGC ACACCCGGAA TGAAACCAAA AATGAGCTGG AGCGTCTGCT GAAACTGCAT
    GAGGATCACA CAGCATATTT GGCCAATGAT GAGGTCACTA CAGTGCGCAA AAACCTGGAG
    GCCCGAGGGG TGGAGGTGGA CCCTGTATTG ATTAAGGACA CATGGCATCA GCTCTTTCGA
    CGTCACTTCC TACAGAAGGC ACTACTACAT TGCAACCTCT GCAGACGAGG TTTCTACTAC
    TATCAGAGGC ACTTCGTAGA CTCTGAGCTT GAGTGCAATG ATGTGGTTCT GTTCTGGAGG
    ATTCAGAGGA TGTTGGGCAT CACTGCCAAT ACTCTTCGAC AACAGCTCAC TAACACTGAG
    GTACGGCGTT TGGAGAAGAA CGTTAAAGAA GTATTAGAAG ACTTTGGAGA GGACAATGAG
    AAAAAAGTCC AGCTGATCAC AGGCAGGAGG GTTCAACTGG CAGAAGACCT CAAAAAAGTT
    CGCGAAATTC AGGAGAAACT TGAAGCCTTC ATTGAAGCTC TTCACAAAGA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332522.1
    CDS174..3230
    Translation

    Target ORF information:

    RefSeq Version XM_021476847.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGCAGGGGCT GCTCAGCGAA
    CTCATTGTCA TTCAGCAGCA GATCCAGCAG CATGAGGAGG AGATGCGGCG CGCGGCAGCC
    AATAACGCTC CGCCTCCGCC CCGTGACCCC AGTCCCAGCC CGGCCCCGAA CCCCAGCCCC
    TCGCAGTCCA AACGCAAGTC ATCCGATAAA GAGAAAGTTG ACCAGCTTCA GGAGGAACTT
    CTTCGAACTC AGTTGAAGTA CCAACGGATG CTTGAGCGCT TGGAAAAGGA AAATAAAGAA
    TTGAGGAAAG TTGTATTGCA GAAGGATGAC AAGGGTATTC ACCAAAGAAA AGTCAAGAAA
    TCTTTGATTG ATATGTACTC TGAGGTCCTG GACATTCTTT CAGATTATGA CTCCAACTAC
    AACACTCAAG ATCACCTGCC AAGGGTGGTG GTAGTTGGGG ACCAGAGTGC GGGAAAGACT
    AGTGTGCTGG AGATGATTGC TCAAGCTAGG ATCTTTCCGA GAGGCTCTGG AGAAATGATG
    ACAAGGTCTC CTGTCAAGGT GACTTTGAGT GAAGGCCCTC ACCATGTAGC AATGTTTAAA
    GACAGCTCTC GGGAGTTTGA CCTTGGCAAA GAGGAAGATC TTGCAGCACT GAGGCATGAG
    ATTGAGCTCA GGATGAGGAA GAGTGTGAAG GAAGGTCAGA CAGTCAGCCC TGAGACCATC
    TCTTTAAGTG TTAAGGGCCC TGGAATCCAG CGAATGGTGC TTGTAGATTT ACCAGGGGTC
    ATCAGTACTG TGACAACTGG AATGGCCGCA GACACAAAGG AAACCATTTT TAGCATTAGT
    AAAGCTTACA TGCAGAATCC AAACGCCATC ATCCTCTGTA TACAGGATGG CTCAGTAGAT
    GCAGAGCGCA GTATAGTCAC TGATCTGGTC AGTCAAATGG ACCCTCAGGG CAAAAGGACC
    ATATTTGTTC TTACTAAGGT TGATCTGGCA GAAAAGAACC TGGCTAGTCC AAGCAGAATC
    CAGCAAATAG TAGAAGGCAA ATTATTCCCA ATGAAGGCTT TGGGCTACTT TGCTGTAGTA
    ACTGGCAAAG GCAGCCCTAA TGAGAGCATA GATTCCATTA AAGACTATGA GGAGGACTTC
    TTCCAGAACT CAAGACTGCT GAAGGATGGC ATGTTGAAAG CTCATCAGGT GACTACAAAG
    AACCTGAGTC TTGCTGTGTC AGACTGTTTC TGGAAAATGG TCAGAGAGTC AGTAGAGCAA
    CAAGCAGATG CCTTTAAAGC TTCTCGATTT AACCTGGAGA CTGAGTGGAA GAACAACTAT
    CCTCGCTTAC GAGAGCTGGA CAGGAACGAG CTCTACGAGA AGGCCAAAAA TGAGATCTTG
    GATGAGGTGA TCAGCTTGAG TCAAGTTACA CCAAAACACT GGGAGTCTAT CTTACAGAAG
    AAGCTTTGGG AAAGAGTCTC GACACACGTC ATAGAGAACA TCTACCTGCC TGCTGCACAG
    ACCATGAACT CTGGAACTTT TAACACCACT GTAGACATCA AACTCAAGCA ATGGACTGAT
    AAACAACTTC CTCATAAAGC ACTAGAGGTT GCTTGGGAGA CTTTGCAGGA AGAGTTTGCC
    CGCTTCATGG CCGAATACAA AGGGAAAGAC CAGGATGACA TTTTTGACAA GCTGAAGGAA
    GCTGTCAAGG ACGAGAGCAT CAAACGACAC AAGTGGAATG AAAGAGCCAT GGACAGCTTG
    AGAGTGATTC AACACAACGC TCTGGAGGAT CGTTCAATCA CAGACAAGCC CCAGTGGGAT
    GCCGCTATAC AGTTCATGGA GGAAACGCTG CAGTCTCGTC TTAAAGACAC TGAGTCAGTA
    ATAGCTGATA TGGTGGGGCC AGACTGGAAG CAGAGGTGGA TGAGCTGGAA AAACCGAACA
    CCAGAGCAGC ACACCCGGAA TGAAACCAAA AATGAGCTGG AGCGTCTGCT GAAACTGCAT
    GAGGATCACA CAGCATATTT GGCCAATGAT GAGGTCACTA CAGTGCGCAA AAACCTGGAG
    GCCCGAGGGG TGGAGGTGGA CCCTGTATTG ATTAAGGACA CATGGCATCA GCTCTTTCGA
    CGTCACTTCC TACAGAAGGC ACTACTACAT TGCAACCTCT GCAGACGAGG TTTCTACTAC
    TATCAGAGGC ACTTCGTAGA CTCTGAGCTT GAGTGCAATG ATGTGGTTCT GTTCTGGAGG
    ATTCAGAGGA TGTTGGGCAT CACTGCCAAT ACTCTTCGAC AACAGCTCAC TAACACTGAG
    GTACGGCGTT TGGAGAAGAA CGTTAAAGAA GTATTAGAAG ACTTTGGAGA GGACAATGAG
    AAAAAAGTCC AGCTGATCAC AGGCAGGAGG GTTCAACTGG CAGAAGACCT CAAAAAAGTT
    CGCGAAATTC AGGAGAAACT TGAAGCCTTC ATTGAAGCTC TTCACAAAGA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa142954
    Clone ID Related Accession (Same CDS sequence) XM_021476848.1
    Accession Version XM_021476848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGGGGCTGCT CAGCGAACTC
    ATTGTCATTC AGCAGCAGAT CCAGCAGCAT GAGGAGGAGA TGCGGCGCGC GGCAGCCAAT
    AACGCTCCGC CTCCGCCCCG TGACCCCAGT CCCAGCCCGG CCCCGAACCC CAGCCCCTCG
    CAGTCCAAAC GCAAGTCATC CGATAAAGAG AAAGTTGACC AGCTTCAGGA GGAACTTCTT
    CGAACTCAGT TGAAGTACCA ACGGATGCTT GAGCGCTTGG AAAAGGAAAA TAAAGAATTG
    AGGAAAGTTG TATTGCAGAA GGATGACAAG GGTATTCACC AAAGAAAAGT CAAGAAATCT
    TTGATTGATA TGTACTCTGA GGTCCTGGAC ATTCTTTCAG ATTATGACTC CAACTACAAC
    ACTCAAGATC ACCTGCCAAG GGTGGTGGTA GTTGGGGACC AGAGTGCGGG AAAGACTAGT
    GTGCTGGAGA TGATTGCTCA AGCTAGGATC TTTCCGAGAG GCTCTGGAGA AATGATGACA
    AGGTCTCCTG TCAAGGTGAC TTTGAGTGAA GGCCCTCACC ATGTAGCAAT GTTTAAAGAC
    AGCTCTCGGG AGTTTGACCT TGGCAAAGAG GAAGATCTTG CAGCACTGAG GCATGAGATT
    GAGCTCAGGA TGAGGAAGAG TGTGAAGGAA GGTCAGACAG TCAGCCCTGA GACCATCTCT
    TTAAGTGTTA AGGGCCCTGG AATCCAGCGA ATGGTGCTTG TAGATTTACC AGGGGTCATC
    AGTACTGTGA CAACTGGAAT GGCCGCAGAC ACAAAGGAAA CCATTTTTAG CATTAGTAAA
    GCTTACATGC AGAATCCAAA CGCCATCATC CTCTGTATAC AGGATGGCTC AGTAGATGCA
    GAGCGCAGTA TAGTCACTGA TCTGGTCAGT CAAATGGACC CTCAGGGCAA AAGGACCATA
    TTTGTTCTTA CTAAGGTTGA TCTGGCAGAA AAGAACCTGG CTAGTCCAAG CAGAATCCAG
    CAAATAGTAG AAGGCAAATT ATTCCCAATG AAGGCTTTGG GCTACTTTGC TGTAGTAACT
    GGCAAAGGCA GCCCTAATGA GAGCATAGAT TCCATTAAAG ACTATGAGGA GGACTTCTTC
    CAGAACTCAA GACTGCTGAA GGATGGCATG TTGAAAGCTC ATCAGGTGAC TACAAAGAAC
    CTGAGTCTTG CTGTGTCAGA CTGTTTCTGG AAAATGGTCA GAGAGTCAGT AGAGCAACAA
    GCAGATGCCT TTAAAGCTTC TCGATTTAAC CTGGAGACTG AGTGGAAGAA CAACTATCCT
    CGCTTACGAG AGCTGGACAG GAACGAGCTC TACGAGAAGG CCAAAAATGA GATCTTGGAT
    GAGGTGATCA GCTTGAGTCA AGTTACACCA AAACACTGGG AGTCTATCTT ACAGAAGAAG
    CTTTGGGAAA GAGTCTCGAC ACACGTCATA GAGAACATCT ACCTGCCTGC TGCACAGACC
    ATGAACTCTG GAACTTTTAA CACCACTGTA GACATCAAAC TCAAGCAATG GACTGATAAA
    CAACTTCCTC ATAAAGCACT AGAGGTTGCT TGGGAGACTT TGCAGGAAGA GTTTGCCCGC
    TTCATGGCCG AATACAAAGG GAAAGACCAG GATGACATTT TTGACAAGCT GAAGGAAGCT
    GTCAAGGACG AGAGCATCAA ACGACACAAG TGGAATGAAA GAGCCATGGA CAGCTTGAGA
    GTGATTCAAC ACAACGCTCT GGAGGATCGT TCAATCACAG ACAAGCCCCA GTGGGATGCC
    GCTATACAGT TCATGGAGGA AACGCTGCAG TCTCGTCTTA AAGACACTGA GTCAGTAATA
    GCTGATATGG TGGGGCCAGA CTGGAAGCAG AGGTGGATGA GCTGGAAAAA CCGAACACCA
    GAGCAGCACA CCCGGAATGA AACCAAAAAT GAGCTGGAGC GTCTGCTGAA ACTGCATGAG
    GATCACACAG CATATTTGGC CAATGATGAG GTCACTACAG TGCGCAAAAA CCTGGAGGCC
    CGAGGGGTGG AGGTGGACCC TGTATTGATT AAGGACACAT GGCATCAGCT CTTTCGACGT
    CACTTCCTAC AGAAGGCACT ACTACATTGC AACCTCTGCA GACGAGGTTT CTACTACTAT
    CAGAGGCACT TCGTAGACTC TGAGCTTGAG TGCAATGATG TGGTTCTGTT CTGGAGGATT
    CAGAGGATGT TGGGCATCAC TGCCAATACT CTTCGACAAC AGCTCACTAA CACTGAGGTA
    CGGCGTTTGG AGAAGAACGT TAAAGAAGTA TTAGAAGACT TTGGAGAGGA CAATGAGAAA
    AAAGTCCAGC TGATCACAGG CAGGAGGGTT CAACTGGCAG AAGACCTCAA AAAAGTTCGC
    GAAATTCAGG AGAAACTTGA AGCCTTCATT GAAGCTCTTC ACAAAGAGAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332523.1
    CDS174..3227
    Translation

    Target ORF information:

    RefSeq Version XM_021476848.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGAAACTAC TGGCGAATCG GCCCTAAGAG CACCAGACGT CCCACCAGCA
    TCTGCAGCCA TGGCTGACAG CGGAGACAAG CAGTTCAAGA AGGGGCTGCT CAGCGAACTC
    ATTGTCATTC AGCAGCAGAT CCAGCAGCAT GAGGAGGAGA TGCGGCGCGC GGCAGCCAAT
    AACGCTCCGC CTCCGCCCCG TGACCCCAGT CCCAGCCCGG CCCCGAACCC CAGCCCCTCG
    CAGTCCAAAC GCAAGTCATC CGATAAAGAG AAAGTTGACC AGCTTCAGGA GGAACTTCTT
    CGAACTCAGT TGAAGTACCA ACGGATGCTT GAGCGCTTGG AAAAGGAAAA TAAAGAATTG
    AGGAAAGTTG TATTGCAGAA GGATGACAAG GGTATTCACC AAAGAAAAGT CAAGAAATCT
    TTGATTGATA TGTACTCTGA GGTCCTGGAC ATTCTTTCAG ATTATGACTC CAACTACAAC
    ACTCAAGATC ACCTGCCAAG GGTGGTGGTA GTTGGGGACC AGAGTGCGGG AAAGACTAGT
    GTGCTGGAGA TGATTGCTCA AGCTAGGATC TTTCCGAGAG GCTCTGGAGA AATGATGACA
    AGGTCTCCTG TCAAGGTGAC TTTGAGTGAA GGCCCTCACC ATGTAGCAAT GTTTAAAGAC
    AGCTCTCGGG AGTTTGACCT TGGCAAAGAG GAAGATCTTG CAGCACTGAG GCATGAGATT
    GAGCTCAGGA TGAGGAAGAG TGTGAAGGAA GGTCAGACAG TCAGCCCTGA GACCATCTCT
    TTAAGTGTTA AGGGCCCTGG AATCCAGCGA ATGGTGCTTG TAGATTTACC AGGGGTCATC
    AGTACTGTGA CAACTGGAAT GGCCGCAGAC ACAAAGGAAA CCATTTTTAG CATTAGTAAA
    GCTTACATGC AGAATCCAAA CGCCATCATC CTCTGTATAC AGGATGGCTC AGTAGATGCA
    GAGCGCAGTA TAGTCACTGA TCTGGTCAGT CAAATGGACC CTCAGGGCAA AAGGACCATA
    TTTGTTCTTA CTAAGGTTGA TCTGGCAGAA AAGAACCTGG CTAGTCCAAG CAGAATCCAG
    CAAATAGTAG AAGGCAAATT ATTCCCAATG AAGGCTTTGG GCTACTTTGC TGTAGTAACT
    GGCAAAGGCA GCCCTAATGA GAGCATAGAT TCCATTAAAG ACTATGAGGA GGACTTCTTC
    CAGAACTCAA GACTGCTGAA GGATGGCATG TTGAAAGCTC ATCAGGTGAC TACAAAGAAC
    CTGAGTCTTG CTGTGTCAGA CTGTTTCTGG AAAATGGTCA GAGAGTCAGT AGAGCAACAA
    GCAGATGCCT TTAAAGCTTC TCGATTTAAC CTGGAGACTG AGTGGAAGAA CAACTATCCT
    CGCTTACGAG AGCTGGACAG GAACGAGCTC TACGAGAAGG CCAAAAATGA GATCTTGGAT
    GAGGTGATCA GCTTGAGTCA AGTTACACCA AAACACTGGG AGTCTATCTT ACAGAAGAAG
    CTTTGGGAAA GAGTCTCGAC ACACGTCATA GAGAACATCT ACCTGCCTGC TGCACAGACC
    ATGAACTCTG GAACTTTTAA CACCACTGTA GACATCAAAC TCAAGCAATG GACTGATAAA
    CAACTTCCTC ATAAAGCACT AGAGGTTGCT TGGGAGACTT TGCAGGAAGA GTTTGCCCGC
    TTCATGGCCG AATACAAAGG GAAAGACCAG GATGACATTT TTGACAAGCT GAAGGAAGCT
    GTCAAGGACG AGAGCATCAA ACGACACAAG TGGAATGAAA GAGCCATGGA CAGCTTGAGA
    GTGATTCAAC ACAACGCTCT GGAGGATCGT TCAATCACAG ACAAGCCCCA GTGGGATGCC
    GCTATACAGT TCATGGAGGA AACGCTGCAG TCTCGTCTTA AAGACACTGA GTCAGTAATA
    GCTGATATGG TGGGGCCAGA CTGGAAGCAG AGGTGGATGA GCTGGAAAAA CCGAACACCA
    GAGCAGCACA CCCGGAATGA AACCAAAAAT GAGCTGGAGC GTCTGCTGAA ACTGCATGAG
    GATCACACAG CATATTTGGC CAATGATGAG GTCACTACAG TGCGCAAAAA CCTGGAGGCC
    CGAGGGGTGG AGGTGGACCC TGTATTGATT AAGGACACAT GGCATCAGCT CTTTCGACGT
    CACTTCCTAC AGAAGGCACT ACTACATTGC AACCTCTGCA GACGAGGTTT CTACTACTAT
    CAGAGGCACT TCGTAGACTC TGAGCTTGAG TGCAATGATG TGGTTCTGTT CTGGAGGATT
    CAGAGGATGT TGGGCATCAC TGCCAATACT CTTCGACAAC AGCTCACTAA CACTGAGGTA
    CGGCGTTTGG AGAAGAACGT TAAAGAAGTA TTAGAAGACT TTGGAGAGGA CAATGAGAAA
    AAAGTCCAGC TGATCACAGG CAGGAGGGTT CAACTGGCAG AAGACCTCAA AAAAGTTCGC
    GAAATTCAGG AGAAACTTGA AGCCTTCATT GAAGCTCTTC ACAAAGAGAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59353
    Clone ID Related Accession (Same CDS sequence) XM_017356476.1 , XM_017356475.1
    Accession Version XM_017356476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGCAGGG GCTGCTCAGC
    GAACTCATTG TCATTCAGCA GCAGATCCAG CAGCATGAGG AGGAGATGCG GCGCGCGGCA
    GCCAATAACG CTCCGCCTCC GCCCCGTGAC CCCAGTCCCA GCCCGGCCCC GAACCCCAGC
    CCCTCGCAGT CCAAACGCAA GTCATCCGAT AAAGAGAAAG TTGACCAGCT TCAGGAGGAA
    CTTCTTCGAA CTCAGTTGAA GTACCAACGG ATGCTTGAGC GCTTGGAAAA GGAAAATAAA
    GAATTGAGGA AAGTTGTATT GCAGAAGGAT GACAAGGGTA TTCACCAAAG AAAAGTCAAG
    AAATCTTTGA TTGATATGTA CTCTGAGGTC CTGGACATTC TTTCAGATTA TGACTCCAAC
    TACAACACTC AAGATCACCT GCCAAGGGTG GTGGTAGTTG GGGACCAGAG TGCGGGAAAG
    ACTAGTGTGC TGGAGATGAT TGCTCAAGCT AGGATCTTTC CGAGAGGCTC TGGAGAAATG
    ATGACAAGGT CTCCTGTCAA GGTGACTTTG AGTGAAGGCC CTCACCATGT AGCAATGTTT
    AAAGACAGCT CTCGGGAGTT TGACCTTGGC AAAGAGGAAG ATCTTGCAGC ACTGAGGCAT
    GAGATTGAGC TCAGGATGAG GAAGAGTGTG AAGGAAGGTC AGACAGTCAG CCCTGAGACC
    ATCTCTTTAA GTGTTAAGGG CCCTGGAATC CAGCGAATGG TGCTTGTAGA TTTACCAGGG
    GTCATCAGTA CTGTGACAAC TGGAATGGCC GCAGACACAA AGGAAACCAT TTTTAGCATT
    AGTAAAGCTT ACATGCAGAA TCCAAACGCC ATCATCCTCT GTATACAGGA TGGCTCAGTA
    GATGCAGAGC GCAGTATAGT CACTGATCTG GTCAGTCAAA TGGACCCTCA GGGCAAAAGG
    ACCATATTTG TTCTTACTAA GGTTGATCTG GCAGAAAAGA ACCTGGCTAG TCCAAGCAGA
    ATCCAGCAAA TAGTAGAAGG CAAATTATTC CCAATGAAGG CTTTGGGCTA CTTTGCTGTA
    GTAACTGGCA AAGGCAGCCC TAATGAGAGC ATAGATTCCA TTAAAGACTA TGAGGAGGAC
    TTCTTCCAGA ACTCAAGACT GCTGAAGGAT GGCATGTTGA AAGCTCATCA GGTGACTACA
    AAGAACCTGA GTCTTGCTGT GTCAGACTGT TTCTGGAAAA TGGTCAGAGA GTCAGTAGAG
    CAACAAGCAG ATGCCTTTAA AGCTTCTCGA TTTAACCTGG AGACTGAGTG GAAGAACAAC
    TATCCTCGCT TACGAGAGCT GGACAGGAAC GAGCTCTACG AGAAGGCCAA AAATGAGATC
    TTGGATGAGG TGATCAGCTT GAGTCAAGTT ACACCAAAAC ACTGGGAGTC TATCTTACAG
    AAGAAGCTTT GGGAAAGAGT CTCGACACAC GTCATAGAGA ACATCTACCT GCCTGCTGCA
    CAGACCATGA ACTCTGGAAC TTTTAACACC ACTGTAGACA TCAAACTCAA GCAATGGACT
    GATAAACAAC TTCCTCATAA AGCACTAGAG GTTGCTTGGG AGACTTTGCA GGAAGAGTTT
    GCCCGCTTCA TGGCCGAATA CAAAGGGAAA GACCAGGATG ACATTTTTGA CAAGCTGAAG
    GAAGCTGTCA AGGACGAGAG CATCAAACGA CACAAGTGGA ATGAAAGAGC CATGGACAGC
    TTGAGAGTGA TTCAACACAA CGCTCTGGAG GATCGTTCAA TCACAGACAA GCCCCAGTGG
    GATGCCGCTA TACAGTTCAT GGAGGAAACG CTGCAGTCTC GTCTTAAAGA CACTGAGTCA
    GTAATAGCTG ATATGGTGGG GCCAGACTGG AAGCAGAGGT GGATGAGCTG GAAAAACCGA
    ACACCAGAGC AGCACACCCG GAATGAAACC AAAAATGAGC TGGAGCGTCT GCTGAAACTG
    CATGAGGATC ACACAGCATA TTTGGCCAAT GATGAGGTCA CTACAGTGCG CAAAAACCTG
    GAGGCCCGAG GGGTGGAGGT GGACCCTGTA TTGATTAAGG ACACATGGCA TCAGCTCTTT
    CGACGTCACT TCCTACAGAA GGCACTACTA CATTGCAACC TCTGCAGACG AGGTTTCTAC
    TACTATCAGA GGCACTTCGT AGACTCTGAG CTTGAGTGCA ATGATGTGGT TCTGTTCTGG
    AGGATTCAGA GGATGTTGGG CATCACTGCC AATACTCTTC GACAACAGCT CACTAACACT
    GAGGTACGGC GTTTGGAGAA GAACGTTAAA GAAGTATTAG AAGACTTTGG AGAGGACAAT
    GAGAAAAAAG TCCAGCTGAT CACAGGCAGG AGGGTTCAAC TGGCAGAAGA CCTCAAAAAA
    GTTCGCGAAA TTCAGGAGAA ACTTGAAGCC TTCATTGAAG CTCTTCACAA AGAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211965.1
    CDS41..3160
    Translation

    Target ORF information:

    RefSeq Version XM_017356476.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGCAGGG GCTGCTCAGC
    GAACTCATTG TCATTCAGCA GCAGATCCAG CAGCATGAGG AGGAGATGCG GCGCGCGGCA
    GCCAATAACG CTCCGCCTCC GCCCCGTGAC CCCAGTCCCA GCCCGGCCCC GAACCCCAGC
    CCCTCGCAGT CCAAACGCAA GTCATCCGAT AAAGAGAAAG TTGACCAGCT TCAGGAGGAA
    CTTCTTCGAA CTCAGTTGAA GTACCAACGG ATGCTTGAGC GCTTGGAAAA GGAAAATAAA
    GAATTGAGGA AAGTTGTATT GCAGAAGGAT GACAAGGGTA TTCACCAAAG AAAAGTCAAG
    AAATCTTTGA TTGATATGTA CTCTGAGGTC CTGGACATTC TTTCAGATTA TGACTCCAAC
    TACAACACTC AAGATCACCT GCCAAGGGTG GTGGTAGTTG GGGACCAGAG TGCGGGAAAG
    ACTAGTGTGC TGGAGATGAT TGCTCAAGCT AGGATCTTTC CGAGAGGCTC TGGAGAAATG
    ATGACAAGGT CTCCTGTCAA GGTGACTTTG AGTGAAGGCC CTCACCATGT AGCAATGTTT
    AAAGACAGCT CTCGGGAGTT TGACCTTGGC AAAGAGGAAG ATCTTGCAGC ACTGAGGCAT
    GAGATTGAGC TCAGGATGAG GAAGAGTGTG AAGGAAGGTC AGACAGTCAG CCCTGAGACC
    ATCTCTTTAA GTGTTAAGGG CCCTGGAATC CAGCGAATGG TGCTTGTAGA TTTACCAGGG
    GTCATCAGTA CTGTGACAAC TGGAATGGCC GCAGACACAA AGGAAACCAT TTTTAGCATT
    AGTAAAGCTT ACATGCAGAA TCCAAACGCC ATCATCCTCT GTATACAGGA TGGCTCAGTA
    GATGCAGAGC GCAGTATAGT CACTGATCTG GTCAGTCAAA TGGACCCTCA GGGCAAAAGG
    ACCATATTTG TTCTTACTAA GGTTGATCTG GCAGAAAAGA ACCTGGCTAG TCCAAGCAGA
    ATCCAGCAAA TAGTAGAAGG CAAATTATTC CCAATGAAGG CTTTGGGCTA CTTTGCTGTA
    GTAACTGGCA AAGGCAGCCC TAATGAGAGC ATAGATTCCA TTAAAGACTA TGAGGAGGAC
    TTCTTCCAGA ACTCAAGACT GCTGAAGGAT GGCATGTTGA AAGCTCATCA GGTGACTACA
    AAGAACCTGA GTCTTGCTGT GTCAGACTGT TTCTGGAAAA TGGTCAGAGA GTCAGTAGAG
    CAACAAGCAG ATGCCTTTAA AGCTTCTCGA TTTAACCTGG AGACTGAGTG GAAGAACAAC
    TATCCTCGCT TACGAGAGCT GGACAGGAAC GAGCTCTACG AGAAGGCCAA AAATGAGATC
    TTGGATGAGG TGATCAGCTT GAGTCAAGTT ACACCAAAAC ACTGGGAGTC TATCTTACAG
    AAGAAGCTTT GGGAAAGAGT CTCGACACAC GTCATAGAGA ACATCTACCT GCCTGCTGCA
    CAGACCATGA ACTCTGGAAC TTTTAACACC ACTGTAGACA TCAAACTCAA GCAATGGACT
    GATAAACAAC TTCCTCATAA AGCACTAGAG GTTGCTTGGG AGACTTTGCA GGAAGAGTTT
    GCCCGCTTCA TGGCCGAATA CAAAGGGAAA GACCAGGATG ACATTTTTGA CAAGCTGAAG
    GAAGCTGTCA AGGACGAGAG CATCAAACGA CACAAGTGGA ATGAAAGAGC CATGGACAGC
    TTGAGAGTGA TTCAACACAA CGCTCTGGAG GATCGTTCAA TCACAGACAA GCCCCAGTGG
    GATGCCGCTA TACAGTTCAT GGAGGAAACG CTGCAGTCTC GTCTTAAAGA CACTGAGTCA
    GTAATAGCTG ATATGGTGGG GCCAGACTGG AAGCAGAGGT GGATGAGCTG GAAAAACCGA
    ACACCAGAGC AGCACACCCG GAATGAAACC AAAAATGAGC TGGAGCGTCT GCTGAAACTG
    CATGAGGATC ACACAGCATA TTTGGCCAAT GATGAGGTCA CTACAGTGCG CAAAAACCTG
    GAGGCCCGAG GGGTGGAGGT GGACCCTGTA TTGATTAAGG ACACATGGCA TCAGCTCTTT
    CGACGTCACT TCCTACAGAA GGCACTACTA CATTGCAACC TCTGCAGACG AGGTTTCTAC
    TACTATCAGA GGCACTTCGT AGACTCTGAG CTTGAGTGCA ATGATGTGGT TCTGTTCTGG
    AGGATTCAGA GGATGTTGGG CATCACTGCC AATACTCTTC GACAACAGCT CACTAACACT
    GAGGTACGGC GTTTGGAGAA GAACGTTAAA GAAGTATTAG AAGACTTTGG AGAGGACAAT
    GAGAAAAAAG TCCAGCTGAT CACAGGCAGG AGGGTTCAAC TGGCAGAAGA CCTCAAAAAA
    GTTCGCGAAA TTCAGGAGAA ACTTGAAGCC TTCATTGAAG CTCTTCACAA AGAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59353
    Clone ID Related Accession (Same CDS sequence) XM_017356476.1 , XM_017356475.1
    Accession Version XM_017356475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3120bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGCAGGG GCTGCTCAGC
    GAACTCATTG TCATTCAGCA GCAGATCCAG CAGCATGAGG AGGAGATGCG GCGCGCGGCA
    GCCAATAACG CTCCGCCTCC GCCCCGTGAC CCCAGTCCCA GCCCGGCCCC GAACCCCAGC
    CCCTCGCAGT CCAAACGCAA GTCATCCGAT AAAGAGAAAG TTGACCAGCT TCAGGAGGAA
    CTTCTTCGAA CTCAGTTGAA GTACCAACGG ATGCTTGAGC GCTTGGAAAA GGAAAATAAA
    GAATTGAGGA AAGTTGTATT GCAGAAGGAT GACAAGGGTA TTCACCAAAG AAAAGTCAAG
    AAATCTTTGA TTGATATGTA CTCTGAGGTC CTGGACATTC TTTCAGATTA TGACTCCAAC
    TACAACACTC AAGATCACCT GCCAAGGGTG GTGGTAGTTG GGGACCAGAG TGCGGGAAAG
    ACTAGTGTGC TGGAGATGAT TGCTCAAGCT AGGATCTTTC CGAGAGGCTC TGGAGAAATG
    ATGACAAGGT CTCCTGTCAA GGTGACTTTG AGTGAAGGCC CTCACCATGT AGCAATGTTT
    AAAGACAGCT CTCGGGAGTT TGACCTTGGC AAAGAGGAAG ATCTTGCAGC ACTGAGGCAT
    GAGATTGAGC TCAGGATGAG GAAGAGTGTG AAGGAAGGTC AGACAGTCAG CCCTGAGACC
    ATCTCTTTAA GTGTTAAGGG CCCTGGAATC CAGCGAATGG TGCTTGTAGA TTTACCAGGG
    GTCATCAGTA CTGTGACAAC TGGAATGGCC GCAGACACAA AGGAAACCAT TTTTAGCATT
    AGTAAAGCTT ACATGCAGAA TCCAAACGCC ATCATCCTCT GTATACAGGA TGGCTCAGTA
    GATGCAGAGC GCAGTATAGT CACTGATCTG GTCAGTCAAA TGGACCCTCA GGGCAAAAGG
    ACCATATTTG TTCTTACTAA GGTTGATCTG GCAGAAAAGA ACCTGGCTAG TCCAAGCAGA
    ATCCAGCAAA TAGTAGAAGG CAAATTATTC CCAATGAAGG CTTTGGGCTA CTTTGCTGTA
    GTAACTGGCA AAGGCAGCCC TAATGAGAGC ATAGATTCCA TTAAAGACTA TGAGGAGGAC
    TTCTTCCAGA ACTCAAGACT GCTGAAGGAT GGCATGTTGA AAGCTCATCA GGTGACTACA
    AAGAACCTGA GTCTTGCTGT GTCAGACTGT TTCTGGAAAA TGGTCAGAGA GTCAGTAGAG
    CAACAAGCAG ATGCCTTTAA AGCTTCTCGA TTTAACCTGG AGACTGAGTG GAAGAACAAC
    TATCCTCGCT TACGAGAGCT GGACAGGAAC GAGCTCTACG AGAAGGCCAA AAATGAGATC
    TTGGATGAGG TGATCAGCTT GAGTCAAGTT ACACCAAAAC ACTGGGAGTC TATCTTACAG
    AAGAAGCTTT GGGAAAGAGT CTCGACACAC GTCATAGAGA ACATCTACCT GCCTGCTGCA
    CAGACCATGA ACTCTGGAAC TTTTAACACC ACTGTAGACA TCAAACTCAA GCAATGGACT
    GATAAACAAC TTCCTCATAA AGCACTAGAG GTTGCTTGGG AGACTTTGCA GGAAGAGTTT
    GCCCGCTTCA TGGCCGAATA CAAAGGGAAA GACCAGGATG ACATTTTTGA CAAGCTGAAG
    GAAGCTGTCA AGGACGAGAG CATCAAACGA CACAAGTGGA ATGAAAGAGC CATGGACAGC
    TTGAGAGTGA TTCAACACAA CGCTCTGGAG GATCGTTCAA TCACAGACAA GCCCCAGTGG
    GATGCCGCTA TACAGTTCAT GGAGGAAACG CTGCAGTCTC GTCTTAAAGA CACTGAGTCA
    GTAATAGCTG ATATGGTGGG GCCAGACTGG AAGCAGAGGT GGATGAGCTG GAAAAACCGA
    ACACCAGAGC AGCACACCCG GAATGAAACC AAAAATGAGC TGGAGCGTCT GCTGAAACTG
    CATGAGGATC ACACAGCATA TTTGGCCAAT GATGAGGTCA CTACAGTGCG CAAAAACCTG
    GAGGCCCGAG GGGTGGAGGT GGACCCTGTA TTGATTAAGG ACACATGGCA TCAGCTCTTT
    CGACGTCACT TCCTACAGAA GGCACTACTA CATTGCAACC TCTGCAGACG AGGTTTCTAC
    TACTATCAGA GGCACTTCGT AGACTCTGAG CTTGAGTGCA ATGATGTGGT TCTGTTCTGG
    AGGATTCAGA GGATGTTGGG CATCACTGCC AATACTCTTC GACAACAGCT CACTAACACT
    GAGGTACGGC GTTTGGAGAA GAACGTTAAA GAAGTATTAG AAGACTTTGG AGAGGACAAT
    GAGAAAAAAG TCCAGCTGAT CACAGGCAGG AGGGTTCAAC TGGCAGAAGA CCTCAAAAAA
    GTTCGCGAAA TTCAGGAGAA ACTTGAAGCC TTCATTGAAG CTCTTCACAA AGAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211964.1
    CDS40..3159
    Translation

    Target ORF information:

    RefSeq Version XM_017356475.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGCAGGG GCTGCTCAGC
    GAACTCATTG TCATTCAGCA GCAGATCCAG CAGCATGAGG AGGAGATGCG GCGCGCGGCA
    GCCAATAACG CTCCGCCTCC GCCCCGTGAC CCCAGTCCCA GCCCGGCCCC GAACCCCAGC
    CCCTCGCAGT CCAAACGCAA GTCATCCGAT AAAGAGAAAG TTGACCAGCT TCAGGAGGAA
    CTTCTTCGAA CTCAGTTGAA GTACCAACGG ATGCTTGAGC GCTTGGAAAA GGAAAATAAA
    GAATTGAGGA AAGTTGTATT GCAGAAGGAT GACAAGGGTA TTCACCAAAG AAAAGTCAAG
    AAATCTTTGA TTGATATGTA CTCTGAGGTC CTGGACATTC TTTCAGATTA TGACTCCAAC
    TACAACACTC AAGATCACCT GCCAAGGGTG GTGGTAGTTG GGGACCAGAG TGCGGGAAAG
    ACTAGTGTGC TGGAGATGAT TGCTCAAGCT AGGATCTTTC CGAGAGGCTC TGGAGAAATG
    ATGACAAGGT CTCCTGTCAA GGTGACTTTG AGTGAAGGCC CTCACCATGT AGCAATGTTT
    AAAGACAGCT CTCGGGAGTT TGACCTTGGC AAAGAGGAAG ATCTTGCAGC ACTGAGGCAT
    GAGATTGAGC TCAGGATGAG GAAGAGTGTG AAGGAAGGTC AGACAGTCAG CCCTGAGACC
    ATCTCTTTAA GTGTTAAGGG CCCTGGAATC CAGCGAATGG TGCTTGTAGA TTTACCAGGG
    GTCATCAGTA CTGTGACAAC TGGAATGGCC GCAGACACAA AGGAAACCAT TTTTAGCATT
    AGTAAAGCTT ACATGCAGAA TCCAAACGCC ATCATCCTCT GTATACAGGA TGGCTCAGTA
    GATGCAGAGC GCAGTATAGT CACTGATCTG GTCAGTCAAA TGGACCCTCA GGGCAAAAGG
    ACCATATTTG TTCTTACTAA GGTTGATCTG GCAGAAAAGA ACCTGGCTAG TCCAAGCAGA
    ATCCAGCAAA TAGTAGAAGG CAAATTATTC CCAATGAAGG CTTTGGGCTA CTTTGCTGTA
    GTAACTGGCA AAGGCAGCCC TAATGAGAGC ATAGATTCCA TTAAAGACTA TGAGGAGGAC
    TTCTTCCAGA ACTCAAGACT GCTGAAGGAT GGCATGTTGA AAGCTCATCA GGTGACTACA
    AAGAACCTGA GTCTTGCTGT GTCAGACTGT TTCTGGAAAA TGGTCAGAGA GTCAGTAGAG
    CAACAAGCAG ATGCCTTTAA AGCTTCTCGA TTTAACCTGG AGACTGAGTG GAAGAACAAC
    TATCCTCGCT TACGAGAGCT GGACAGGAAC GAGCTCTACG AGAAGGCCAA AAATGAGATC
    TTGGATGAGG TGATCAGCTT GAGTCAAGTT ACACCAAAAC ACTGGGAGTC TATCTTACAG
    AAGAAGCTTT GGGAAAGAGT CTCGACACAC GTCATAGAGA ACATCTACCT GCCTGCTGCA
    CAGACCATGA ACTCTGGAAC TTTTAACACC ACTGTAGACA TCAAACTCAA GCAATGGACT
    GATAAACAAC TTCCTCATAA AGCACTAGAG GTTGCTTGGG AGACTTTGCA GGAAGAGTTT
    GCCCGCTTCA TGGCCGAATA CAAAGGGAAA GACCAGGATG ACATTTTTGA CAAGCTGAAG
    GAAGCTGTCA AGGACGAGAG CATCAAACGA CACAAGTGGA ATGAAAGAGC CATGGACAGC
    TTGAGAGTGA TTCAACACAA CGCTCTGGAG GATCGTTCAA TCACAGACAA GCCCCAGTGG
    GATGCCGCTA TACAGTTCAT GGAGGAAACG CTGCAGTCTC GTCTTAAAGA CACTGAGTCA
    GTAATAGCTG ATATGGTGGG GCCAGACTGG AAGCAGAGGT GGATGAGCTG GAAAAACCGA
    ACACCAGAGC AGCACACCCG GAATGAAACC AAAAATGAGC TGGAGCGTCT GCTGAAACTG
    CATGAGGATC ACACAGCATA TTTGGCCAAT GATGAGGTCA CTACAGTGCG CAAAAACCTG
    GAGGCCCGAG GGGTGGAGGT GGACCCTGTA TTGATTAAGG ACACATGGCA TCAGCTCTTT
    CGACGTCACT TCCTACAGAA GGCACTACTA CATTGCAACC TCTGCAGACG AGGTTTCTAC
    TACTATCAGA GGCACTTCGT AGACTCTGAG CTTGAGTGCA ATGATGTGGT TCTGTTCTGG
    AGGATTCAGA GGATGTTGGG CATCACTGCC AATACTCTTC GACAACAGCT CACTAACACT
    GAGGTACGGC GTTTGGAGAA GAACGTTAAA GAAGTATTAG AAGACTTTGG AGAGGACAAT
    GAGAAAAAAG TCCAGCTGAT CACAGGCAGG AGGGTTCAAC TGGCAGAAGA CCTCAAAAAA
    GTTCGCGAAA TTCAGGAGAA ACTTGAAGCC TTCATTGAAG CTCTTCACAA AGAGAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59354
    Clone ID Related Accession (Same CDS sequence) XM_017356477.1
    Accession Version XM_017356477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3117bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGGGGCT GCTCAGCGAA
    CTCATTGTCA TTCAGCAGCA GATCCAGCAG CATGAGGAGG AGATGCGGCG CGCGGCAGCC
    AATAACGCTC CGCCTCCGCC CCGTGACCCC AGTCCCAGCC CGGCCCCGAA CCCCAGCCCC
    TCGCAGTCCA AACGCAAGTC ATCCGATAAA GAGAAAGTTG ACCAGCTTCA GGAGGAACTT
    CTTCGAACTC AGTTGAAGTA CCAACGGATG CTTGAGCGCT TGGAAAAGGA AAATAAAGAA
    TTGAGGAAAG TTGTATTGCA GAAGGATGAC AAGGGTATTC ACCAAAGAAA AGTCAAGAAA
    TCTTTGATTG ATATGTACTC TGAGGTCCTG GACATTCTTT CAGATTATGA CTCCAACTAC
    AACACTCAAG ATCACCTGCC AAGGGTGGTG GTAGTTGGGG ACCAGAGTGC GGGAAAGACT
    AGTGTGCTGG AGATGATTGC TCAAGCTAGG ATCTTTCCGA GAGGCTCTGG AGAAATGATG
    ACAAGGTCTC CTGTCAAGGT GACTTTGAGT GAAGGCCCTC ACCATGTAGC AATGTTTAAA
    GACAGCTCTC GGGAGTTTGA CCTTGGCAAA GAGGAAGATC TTGCAGCACT GAGGCATGAG
    ATTGAGCTCA GGATGAGGAA GAGTGTGAAG GAAGGTCAGA CAGTCAGCCC TGAGACCATC
    TCTTTAAGTG TTAAGGGCCC TGGAATCCAG CGAATGGTGC TTGTAGATTT ACCAGGGGTC
    ATCAGTACTG TGACAACTGG AATGGCCGCA GACACAAAGG AAACCATTTT TAGCATTAGT
    AAAGCTTACA TGCAGAATCC AAACGCCATC ATCCTCTGTA TACAGGATGG CTCAGTAGAT
    GCAGAGCGCA GTATAGTCAC TGATCTGGTC AGTCAAATGG ACCCTCAGGG CAAAAGGACC
    ATATTTGTTC TTACTAAGGT TGATCTGGCA GAAAAGAACC TGGCTAGTCC AAGCAGAATC
    CAGCAAATAG TAGAAGGCAA ATTATTCCCA ATGAAGGCTT TGGGCTACTT TGCTGTAGTA
    ACTGGCAAAG GCAGCCCTAA TGAGAGCATA GATTCCATTA AAGACTATGA GGAGGACTTC
    TTCCAGAACT CAAGACTGCT GAAGGATGGC ATGTTGAAAG CTCATCAGGT GACTACAAAG
    AACCTGAGTC TTGCTGTGTC AGACTGTTTC TGGAAAATGG TCAGAGAGTC AGTAGAGCAA
    CAAGCAGATG CCTTTAAAGC TTCTCGATTT AACCTGGAGA CTGAGTGGAA GAACAACTAT
    CCTCGCTTAC GAGAGCTGGA CAGGAACGAG CTCTACGAGA AGGCCAAAAA TGAGATCTTG
    GATGAGGTGA TCAGCTTGAG TCAAGTTACA CCAAAACACT GGGAGTCTAT CTTACAGAAG
    AAGCTTTGGG AAAGAGTCTC GACACACGTC ATAGAGAACA TCTACCTGCC TGCTGCACAG
    ACCATGAACT CTGGAACTTT TAACACCACT GTAGACATCA AACTCAAGCA ATGGACTGAT
    AAACAACTTC CTCATAAAGC ACTAGAGGTT GCTTGGGAGA CTTTGCAGGA AGAGTTTGCC
    CGCTTCATGG CCGAATACAA AGGGAAAGAC CAGGATGACA TTTTTGACAA GCTGAAGGAA
    GCTGTCAAGG ACGAGAGCAT CAAACGACAC AAGTGGAATG AAAGAGCCAT GGACAGCTTG
    AGAGTGATTC AACACAACGC TCTGGAGGAT CGTTCAATCA CAGACAAGCC CCAGTGGGAT
    GCCGCTATAC AGTTCATGGA GGAAACGCTG CAGTCTCGTC TTAAAGACAC TGAGTCAGTA
    ATAGCTGATA TGGTGGGGCC AGACTGGAAG CAGAGGTGGA TGAGCTGGAA AAACCGAACA
    CCAGAGCAGC ACACCCGGAA TGAAACCAAA AATGAGCTGG AGCGTCTGCT GAAACTGCAT
    GAGGATCACA CAGCATATTT GGCCAATGAT GAGGTCACTA CAGTGCGCAA AAACCTGGAG
    GCCCGAGGGG TGGAGGTGGA CCCTGTATTG ATTAAGGACA CATGGCATCA GCTCTTTCGA
    CGTCACTTCC TACAGAAGGC ACTACTACAT TGCAACCTCT GCAGACGAGG TTTCTACTAC
    TATCAGAGGC ACTTCGTAGA CTCTGAGCTT GAGTGCAATG ATGTGGTTCT GTTCTGGAGG
    ATTCAGAGGA TGTTGGGCAT CACTGCCAAT ACTCTTCGAC AACAGCTCAC TAACACTGAG
    GTACGGCGTT TGGAGAAGAA CGTTAAAGAA GTATTAGAAG ACTTTGGAGA GGACAATGAG
    AAAAAAGTCC AGCTGATCAC AGGCAGGAGG GTTCAACTGG CAGAAGACCT CAAAAAAGTT
    CGCGAAATTC AGGAGAAACT TGAAGCCTTC ATTGAAGCTC TTCACAAAGA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211966.1
    CDS40..3156
    Translation

    Target ORF information:

    RefSeq Version XM_017356477.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGGT TTGGGTAGTG AAGTCAATGG AGCTTCTGGT
    CTGCGTCTGT TATTAGAAAC TACTGGCGAA TCGGCCCTAA GAGCACCAGA CGTCCCACCA
    GCATCTGCAG CCATGGCTGA CAGCGGAGAC AAGCAGTTCA AGAAGGGGCT GCTCAGCGAA
    CTCATTGTCA TTCAGCAGCA GATCCAGCAG CATGAGGAGG AGATGCGGCG CGCGGCAGCC
    AATAACGCTC CGCCTCCGCC CCGTGACCCC AGTCCCAGCC CGGCCCCGAA CCCCAGCCCC
    TCGCAGTCCA AACGCAAGTC ATCCGATAAA GAGAAAGTTG ACCAGCTTCA GGAGGAACTT
    CTTCGAACTC AGTTGAAGTA CCAACGGATG CTTGAGCGCT TGGAAAAGGA AAATAAAGAA
    TTGAGGAAAG TTGTATTGCA GAAGGATGAC AAGGGTATTC ACCAAAGAAA AGTCAAGAAA
    TCTTTGATTG ATATGTACTC TGAGGTCCTG GACATTCTTT CAGATTATGA CTCCAACTAC
    AACACTCAAG ATCACCTGCC AAGGGTGGTG GTAGTTGGGG ACCAGAGTGC GGGAAAGACT
    AGTGTGCTGG AGATGATTGC TCAAGCTAGG ATCTTTCCGA GAGGCTCTGG AGAAATGATG
    ACAAGGTCTC CTGTCAAGGT GACTTTGAGT GAAGGCCCTC ACCATGTAGC AATGTTTAAA
    GACAGCTCTC GGGAGTTTGA CCTTGGCAAA GAGGAAGATC TTGCAGCACT GAGGCATGAG
    ATTGAGCTCA GGATGAGGAA GAGTGTGAAG GAAGGTCAGA CAGTCAGCCC TGAGACCATC
    TCTTTAAGTG TTAAGGGCCC TGGAATCCAG CGAATGGTGC TTGTAGATTT ACCAGGGGTC
    ATCAGTACTG TGACAACTGG AATGGCCGCA GACACAAAGG AAACCATTTT TAGCATTAGT
    AAAGCTTACA TGCAGAATCC AAACGCCATC ATCCTCTGTA TACAGGATGG CTCAGTAGAT
    GCAGAGCGCA GTATAGTCAC TGATCTGGTC AGTCAAATGG ACCCTCAGGG CAAAAGGACC
    ATATTTGTTC TTACTAAGGT TGATCTGGCA GAAAAGAACC TGGCTAGTCC AAGCAGAATC
    CAGCAAATAG TAGAAGGCAA ATTATTCCCA ATGAAGGCTT TGGGCTACTT TGCTGTAGTA
    ACTGGCAAAG GCAGCCCTAA TGAGAGCATA GATTCCATTA AAGACTATGA GGAGGACTTC
    TTCCAGAACT CAAGACTGCT GAAGGATGGC ATGTTGAAAG CTCATCAGGT GACTACAAAG
    AACCTGAGTC TTGCTGTGTC AGACTGTTTC TGGAAAATGG TCAGAGAGTC AGTAGAGCAA
    CAAGCAGATG CCTTTAAAGC TTCTCGATTT AACCTGGAGA CTGAGTGGAA GAACAACTAT
    CCTCGCTTAC GAGAGCTGGA CAGGAACGAG CTCTACGAGA AGGCCAAAAA TGAGATCTTG
    GATGAGGTGA TCAGCTTGAG TCAAGTTACA CCAAAACACT GGGAGTCTAT CTTACAGAAG
    AAGCTTTGGG AAAGAGTCTC GACACACGTC ATAGAGAACA TCTACCTGCC TGCTGCACAG
    ACCATGAACT CTGGAACTTT TAACACCACT GTAGACATCA AACTCAAGCA ATGGACTGAT
    AAACAACTTC CTCATAAAGC ACTAGAGGTT GCTTGGGAGA CTTTGCAGGA AGAGTTTGCC
    CGCTTCATGG CCGAATACAA AGGGAAAGAC CAGGATGACA TTTTTGACAA GCTGAAGGAA
    GCTGTCAAGG ACGAGAGCAT CAAACGACAC AAGTGGAATG AAAGAGCCAT GGACAGCTTG
    AGAGTGATTC AACACAACGC TCTGGAGGAT CGTTCAATCA CAGACAAGCC CCAGTGGGAT
    GCCGCTATAC AGTTCATGGA GGAAACGCTG CAGTCTCGTC TTAAAGACAC TGAGTCAGTA
    ATAGCTGATA TGGTGGGGCC AGACTGGAAG CAGAGGTGGA TGAGCTGGAA AAACCGAACA
    CCAGAGCAGC ACACCCGGAA TGAAACCAAA AATGAGCTGG AGCGTCTGCT GAAACTGCAT
    GAGGATCACA CAGCATATTT GGCCAATGAT GAGGTCACTA CAGTGCGCAA AAACCTGGAG
    GCCCGAGGGG TGGAGGTGGA CCCTGTATTG ATTAAGGACA CATGGCATCA GCTCTTTCGA
    CGTCACTTCC TACAGAAGGC ACTACTACAT TGCAACCTCT GCAGACGAGG TTTCTACTAC
    TATCAGAGGC ACTTCGTAGA CTCTGAGCTT GAGTGCAATG ATGTGGTTCT GTTCTGGAGG
    ATTCAGAGGA TGTTGGGCAT CACTGCCAAT ACTCTTCGAC AACAGCTCAC TAACACTGAG
    GTACGGCGTT TGGAGAAGAA CGTTAAAGAA GTATTAGAAG ACTTTGGAGA GGACAATGAG
    AAAAAAGTCC AGCTGATCAC AGGCAGGAGG GTTCAACTGG CAGAAGACCT CAAAAAAGTT
    CGCGAAATTC AGGAGAAACT TGAAGCCTTC ATTGAAGCTC TTCACAAAGA GAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59355
    Clone ID Related Accession (Same CDS sequence) XM_017356478.1
    Accession Version XM_017356478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGAA ACTACTGGCG AATCGGCCCT AAGAGCACCA
    GACGTCCCAC CAGCATCTGC AGCCATGGCT GACAGCGGAG ACAAGCAGTT CAAGAAGCAG
    GGGCTGCTCA GCGAACTCAT TGTCATTCAG CAGCAGATCC AGCAGCATGA GGAGGAGATG
    CGGCGCGCGG CAGCCAATAA CGCTCCGCCT CCGCCCCGTG ACCCCAGTCC CAGCCCGGCC
    CCGAACCCCA GCCCCTCGCA GTCCAAACGC AAGTCATCCG ATAAAGAGAA AGTTGACCAG
    CTTCAGGAGG AACTTCTTCG AACTCAGTTG AAGTACCAAC GGATGCTTGA GCGCTTGGAA
    AAGGAAAATA AAGAATTGAG GAAAGTTGTA TTGCAGAAGG ATGACAAGGG TATTCACCAA
    AGAAAAGTCA AGAAATCTTT GATTGATATG TACTCTGAGG TCCTGGACAT TCTTTCAGAT
    TATGACTCCA ACTACAACAC TCAAGATCAC CTGCCAAGGG TGGTGGTAGT TGGGGACCAG
    AGTGCGGGAA AGACTAGTGT GCTGGAGATG ATTGCTCAAG CTAGGATCTT TCCGAGAGGC
    TCTGGAGAAA TGATGACAAG GTCTCCTGTC AAGGTGACTT TGAGTGAAGG CCCTCACCAT
    GTAGCAATGT TTAAAGACAG CTCTCGGGAG TTTGACCTTG GCAAAGAGGA AGATCTTGCA
    GCACTGAGGC ATGAGATTGA GCTCAGGATG AGGAAGAGTG TGAAGGAAGG TCAGACAGTC
    AGCCCTGAGA CCATCTCTTT AAGTGTTAAG GGCCCTGGAA TCCAGCGAAT GGTGCTTGTA
    GATTTACCAG GGGTCATCAG TACTGTGACA ACTGGAATGG CCGCAGACAC AAAGGAAACC
    ATTTTTAGCA TTAGTAAAGC TTACATGCAG AATCCAAACG CCATCATCCT CTGTATACAG
    GATGGCTCAG TAGATGCAGA GCGCAGTATA GTCACTGATC TGGTCAGTCA AATGGACCCT
    CAGGGCAAAA GGACCATATT TGTTCTTACT AAGGTTGATC TGGCAGAAAA GAACCTGGCT
    AGTCCAAGCA GAATCCAGCA AATAGTAGAA GGCAAATTAT TCCCAATGAA GGCTTTGGGC
    TACTTTGCTG TAGTAACTGG CAAAGGCAGC CCTAATGAGA GCATAGATTC CATTAAAGAC
    TATGAGGAGG ACTTCTTCCA GAACTCAAGA CTGCTGAAGG ATGGCATGTT GAAAGCTCAT
    CAGGTGACTA CAAAGAACCT GAGTCTTGCT GTGTCAGACT GTTTCTGGAA AATGGTCAGA
    GAGTCAGTAG AGCAACAAGC AGATGCCTTT AAAGCTTCTC GATTTAACCT GGAGACTGAG
    TGGAAGAACA ACTATCCTCG CTTACGAGAG CTGGACAGGA ACGAGCTCTA CGAGAAGGCC
    AAAAATGAGA TCTTGGATGA GGTGATCAGC TTGAGTCAAG TTACACCAAA ACACTGGGAG
    TCTATCTTAC AGAAGAAGCT TTGGGAAAGA GTCTCGACAC ACGTCATAGA GAACATCTAC
    CTGCCTGCTG CACAGACCAT GAACTCTGGA ACTTTTAACA CCACTGTAGA CATCAAACTC
    AAGCAATGGA CTGATAAACA ACTTCCTCAT AAAGCACTAG AGGTTGCTTG GGAGACTTTG
    CAGGAAGAGT TTGCCCGCTT CATGGCCGAA TACAAAGGGA AAGACCAGGA TGACATTTTT
    GACAAGCTGA AGGAAGCTGT CAAGGACGAG AGCATCAAAC GACACAAGTG GAATGAAAGA
    GCCATGGACA GCTTGAGAGT GATTCAACAC AACGCTCTGG AGGATCGTTC AATCACAGAC
    AAGCCCCAGT GGGATGCCGC TATACAGTTC ATGGAGGAAA CGCTGCAGTC TCGTCTTAAA
    GACACTGAGT CAGTAATAGC TGATATGGTG GGGCCAGACT GGAAGCAGAG GTGGATGAGC
    TGGAAAAACC GAACACCAGA GCAGCACACC CGGAATGAAA CCAAAAATGA GCTGGAGCGT
    CTGCTGAAAC TGCATGAGGA TCACACAGCA TATTTGGCCA ATGATGAGGT CACTACAGTG
    CGCAAAAACC TGGAGGCCCG AGGGGTGGAG GTGGACCCTG TATTGATTAA GGACACATGG
    CATCAGCTCT TTCGACGTCA CTTCCTACAG AAGGCACTAC TACATTGCAA CCTCTGCAGA
    CGAGGTTTCT ACTACTATCA GAGGCACTTC GTAGACTCTG AGCTTGAGTG CAATGATGTG
    GTTCTGTTCT GGAGGATTCA GAGGATGTTG GGCATCACTG CCAATACTCT TCGACAACAG
    CTCACTAACA CTGAGGTACG GCGTTTGGAG AAGAACGTTA AAGAAGTATT AGAAGACTTT
    GGAGAGGACA ATGAGAAAAA AGTCCAGCTG ATCACAGGCA GGAGGGTTCA ACTGGCAGAA
    GACCTCAAAA AAGTTCGCGA AATTCAGGAG AAACTTGAAG CCTTCATTGA AGCTCTTCAC
    AAAGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211967.1
    CDS40..3111
    Translation

    Target ORF information:

    RefSeq Version XM_017356478.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTATTGTG CAGCTCTAAT CACAGTATAT CTCTCTTTTC CCAGCATTGC CTGCAAGGGT 
    TTGCTTCCCT CCAGGATGGG GGTGAAGTTC AGAGTCCCAT TGCAGAAACT GCACCCGCTC
    TCCCGTGCCA TCCATCACCG CTACTCTGCT AACAACAACC CACAGAGACC ACCTCACTGC
    TCAGCTGCCC GGCATTACAC ATCTCTCTCC AGGCTTCCCA TGCGTCCTCC CAAATCACGC
    TCTGGAGGCC ACGGCTATCA ACAGCATCGG ACCTTCTGGG TGGCCCGTCT CGCCGCCAGA
    CTGCTGAAAC TGAGGTACAT CTTGCTTGGC TCAGCGGTTG GAGGTGGATA CACTGCAAAA
    AAGACCTATG ATGAATGGAA GGAAATGCTC CCTGATATGA GTGAATACAC ATGGATTGTG
    CCAGACTTTG TTTGGGAGCT GAGCGAAAAT ATAGATTTAG ACAAGCTAGC CAGTGCTTTA
    CCTGAATTAG AAGAGATAGC CAAACTACTA CCTGATATGG AGAAGATCGG AGAGAATTTC
    ACCTTCCTGA AGAGCCTCCT CTCCAGCGAA ACTACTGGCG AATCGGCCCT AAGAGCACCA
    GACGTCCCAC CAGCATCTGC AGCCATGGCT GACAGCGGAG ACAAGCAGTT CAAGAAGCAG
    GGGCTGCTCA GCGAACTCAT TGTCATTCAG CAGCAGATCC AGCAGCATGA GGAGGAGATG
    CGGCGCGCGG CAGCCAATAA CGCTCCGCCT CCGCCCCGTG ACCCCAGTCC CAGCCCGGCC
    CCGAACCCCA GCCCCTCGCA GTCCAAACGC AAGTCATCCG ATAAAGAGAA AGTTGACCAG
    CTTCAGGAGG AACTTCTTCG AACTCAGTTG AAGTACCAAC GGATGCTTGA GCGCTTGGAA
    AAGGAAAATA AAGAATTGAG GAAAGTTGTA TTGCAGAAGG ATGACAAGGG TATTCACCAA
    AGAAAAGTCA AGAAATCTTT GATTGATATG TACTCTGAGG TCCTGGACAT TCTTTCAGAT
    TATGACTCCA ACTACAACAC TCAAGATCAC CTGCCAAGGG TGGTGGTAGT TGGGGACCAG
    AGTGCGGGAA AGACTAGTGT GCTGGAGATG ATTGCTCAAG CTAGGATCTT TCCGAGAGGC
    TCTGGAGAAA TGATGACAAG GTCTCCTGTC AAGGTGACTT TGAGTGAAGG CCCTCACCAT
    GTAGCAATGT TTAAAGACAG CTCTCGGGAG TTTGACCTTG GCAAAGAGGA AGATCTTGCA
    GCACTGAGGC ATGAGATTGA GCTCAGGATG AGGAAGAGTG TGAAGGAAGG TCAGACAGTC
    AGCCCTGAGA CCATCTCTTT AAGTGTTAAG GGCCCTGGAA TCCAGCGAAT GGTGCTTGTA
    GATTTACCAG GGGTCATCAG TACTGTGACA ACTGGAATGG CCGCAGACAC AAAGGAAACC
    ATTTTTAGCA TTAGTAAAGC TTACATGCAG AATCCAAACG CCATCATCCT CTGTATACAG
    GATGGCTCAG TAGATGCAGA GCGCAGTATA GTCACTGATC TGGTCAGTCA AATGGACCCT
    CAGGGCAAAA GGACCATATT TGTTCTTACT AAGGTTGATC TGGCAGAAAA GAACCTGGCT
    AGTCCAAGCA GAATCCAGCA AATAGTAGAA GGCAAATTAT TCCCAATGAA GGCTTTGGGC
    TACTTTGCTG TAGTAACTGG CAAAGGCAGC CCTAATGAGA GCATAGATTC CATTAAAGAC
    TATGAGGAGG ACTTCTTCCA GAACTCAAGA CTGCTGAAGG ATGGCATGTT GAAAGCTCAT
    CAGGTGACTA CAAAGAACCT GAGTCTTGCT GTGTCAGACT GTTTCTGGAA AATGGTCAGA
    GAGTCAGTAG AGCAACAAGC AGATGCCTTT AAAGCTTCTC GATTTAACCT GGAGACTGAG
    TGGAAGAACA ACTATCCTCG CTTACGAGAG CTGGACAGGA ACGAGCTCTA CGAGAAGGCC
    AAAAATGAGA TCTTGGATGA GGTGATCAGC TTGAGTCAAG TTACACCAAA ACACTGGGAG
    TCTATCTTAC AGAAGAAGCT TTGGGAAAGA GTCTCGACAC ACGTCATAGA GAACATCTAC
    CTGCCTGCTG CACAGACCAT GAACTCTGGA ACTTTTAACA CCACTGTAGA CATCAAACTC
    AAGCAATGGA CTGATAAACA ACTTCCTCAT AAAGCACTAG AGGTTGCTTG GGAGACTTTG
    CAGGAAGAGT TTGCCCGCTT CATGGCCGAA TACAAAGGGA AAGACCAGGA TGACATTTTT
    GACAAGCTGA AGGAAGCTGT CAAGGACGAG AGCATCAAAC GACACAAGTG GAATGAAAGA
    GCCATGGACA GCTTGAGAGT GATTCAACAC AACGCTCTGG AGGATCGTTC AATCACAGAC
    AAGCCCCAGT GGGATGCCGC TATACAGTTC ATGGAGGAAA CGCTGCAGTC TCGTCTTAAA
    GACACTGAGT CAGTAATAGC TGATATGGTG GGGCCAGACT GGAAGCAGAG GTGGATGAGC
    TGGAAAAACC GAACACCAGA GCAGCACACC CGGAATGAAA CCAAAAATGA GCTGGAGCGT
    CTGCTGAAAC TGCATGAGGA TCACACAGCA TATTTGGCCA ATGATGAGGT CACTACAGTG
    CGCAAAAACC TGGAGGCCCG AGGGGTGGAG GTGGACCCTG TATTGATTAA GGACACATGG
    CATCAGCTCT TTCGACGTCA CTTCCTACAG AAGGCACTAC TACATTGCAA CCTCTGCAGA
    CGAGGTTTCT ACTACTATCA GAGGCACTTC GTAGACTCTG AGCTTGAGTG CAATGATGTG
    GTTCTGTTCT GGAGGATTCA GAGGATGTTG GGCATCACTG CCAATACTCT TCGACAACAG
    CTCACTAACA CTGAGGTACG GCGTTTGGAG AAGAACGTTA AAGAAGTATT AGAAGACTTT
    GGAGAGGACA ATGAGAAAAA AGTCCAGCTG ATCACAGGCA GGAGGGTTCA ACTGGCAGAA
    GACCTCAAAA AAGTTCGCGA AATTCAGGAG AAACTTGAAG CCTTCATTGA AGCTCTTCAC
    AAAGAGAAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12002
    Clone ID Related Accession (Same CDS sequence) XM_021476845.1 , XM_009302514.2 , XM_009302514.3
    Accession Version XM_021476845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021332520.1
    CDS174..3278
    Translation

    Target ORF information:

    RefSeq Version XM_021476845.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12002
    Clone ID Related Accession (Same CDS sequence) XM_021476845.1 , XM_009302514.2 , XM_009302514.3
    Accession Version XM_009302514.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300789.1
    CDS174..3278
    Translation

    Target ORF information:

    RefSeq Version XM_009302514.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302514.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12002
    Clone ID Related Accession (Same CDS sequence) XM_021476845.1 , XM_009302514.2 , XM_009302514.3
    Accession Version XM_009302514.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product dynamin-like 120 kDa protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302514.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300789.1
    CDS174..3278
    Translation

    Target ORF information:

    RefSeq Version XM_009302514.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio optic atrophy 1 (autosomal dominant) (opa1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302514.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTTGCGAG CCGGAAGTGT AGTTACCTGC ATTGCCTGCA AGGGTTTGCT TCCCTCCAGG 
    ATGGGGGTGA AGTTCAGAGT CCCATTGCAG AAACTGCACC CGCTCTCCCG TGCCATCCAT
    CACCGCTACT CTGCTAACAA CAACCCACAG AGACCACCTC ACTGCTCAGC TGCCCGGCAT
    TACACATCTC TCTCCAGGCT TCCCATGCGT CCTCCCAAAT CACGCTCTGG AGGCCACGGC
    TATCAACAGC ATCGGACCTT CTGGGTGGCC CGTCTCGCCG CCAGACTGCT GAAACTGAGG
    TACATCTTGC TTGGCTCAGC GGTTGGAGGT GGATACACTG CAAAAAAGAC CTATGATGAA
    TGGAAGGAAA TGCTCCCTGA TATGAGTGAA TACACATGGA TTGTGCCAGA CTTTGTTTGG
    GAGCTGAGCG AAAATATAGA TTTAGACAAG CTAGCCAGTG CTTTACCTGA ATTAGAAGAG
    ATAGCCAAAC TACTACCTGA TATGGAGAAG ATCGGAGAGA ATTTCACCTT CCTGAAGAGC
    CTCCTCTCCA GCGGTTTGGG TAGTGAAGTC AATGGAGCTT CTGGTCTGCG TCTGTTATTA
    GAAACTACTG GCGAATCGGC CCTAAGAGCA CCAGACGTCC CACCAGCATC TGCAGCCATG
    GCTGACAGCG GAGACAAGCA GTTCAAGAAG CAGGGGCTGC TCAGCGAACT CATTGTCATT
    CAGCAGCAGA TCCAGCAGCA TGAGGAGGAG ATGCGGCGCG CGGCAGCCAA TAACGCTCCG
    CCTCCGCCCC GTGACCCCAG TCCCAGCCCG GCCCCGAACC CCAGCCCCTC GCAGTCCAAA
    CGCAAGTCAT CCGATAAAGA GAAAGTTGAC CAGCTTCAGG AGGAACTTCT TCGAACTCAG
    TTGAAGTACC AACGGATGCT TGAGCGCTTG GAAAAGGAAA ATAAAGAATT GAGGAAAGTT
    GTATTGCAGA AGGATGACAA GGGTATTCAC CAAAGAAAAG TCAAGAAATC TTTGATTGAT
    ATGTACTCTG AGGTCCTGGA CATTCTTTCA GATTATGACT CCAACTACAA CACTCAAGAT
    CACCTGCCAA GGGTGGTGGT AGTTGGGGAC CAGAGTGCGG GAAAGACTAG TGTGCTGGAG
    ATGATTGCTC AAGCTAGGAT CTTTCCGAGA GGCTCTGGAG AAATGATGAC AAGGTCTCCT
    GTCAAGGTGA CTTTGAGTGA AGGCCCTCAC CATGTAGCAA TGTTTAAAGA CAGCTCTCGG
    GAGTTTGACC TTGGCAAAGA GGAAGATCTT GCAGCACTGA GGCATGAGAT TGAGCTCAGG
    ATGAGGAAGA GTGTGAAGGA AGGTCAGACA GTCAGCCCTG AGACCATCTC TTTAAGTGTT
    AAGGGCCCTG GAATCCAGCG AATGGTGCTT GTAGATTTAC CAGGGGTCAT CAGTACTGTG
    ACAACTGGAA TGGCCGCAGA CACAAAGGAA ACCATTTTTA GCATTAGTAA AGCTTACATG
    CAGAATCCAA ACGCCATCAT CCTCTGTATA CAGGATGGCT CAGTAGATGC AGAGCGCAGT
    ATAGTCACTG ATCTGGTCAG TCAAATGGAC CCTCAGGGCA AAAGGACCAT ATTTGTTCTT
    ACTAAGGTTG ATCTGGCAGA AAAGAACCTG GCTAGTCCAA GCAGAATCCA GCAAATAGTA
    GAAGGCAAAT TATTCCCAAT GAAGGCTTTG GGCTACTTTG CTGTAGTAAC TGGCAAAGGC
    AGCCCTAATG AGAGCATAGA TTCCATTAAA GACTATGAGG AGGACTTCTT CCAGAACTCA
    AGACTGCTGA AGGATGGCAT GTTGAAAGCT CATCAGGTGA CTACAAAGAA CCTGAGTCTT
    GCTGTGTCAG ACTGTTTCTG GAAAATGGTC AGAGAGTCAG TAGAGCAACA AGCAGATGCC
    TTTAAAGCTT CTCGATTTAA CCTGGAGACT GAGTGGAAGA ACAACTATCC TCGCTTACGA
    GAGCTGGACA GGAACGAGCT CTACGAGAAG GCCAAAAATG AGATCTTGGA TGAGGTGATC
    AGCTTGAGTC AAGTTACACC AAAACACTGG GAGTCTATCT TACAGAAGAA GCTTTGGGAA
    AGAGTCTCGA CACACGTCAT AGAGAACATC TACCTGCCTG CTGCACAGAC CATGAACTCT
    GGAACTTTTA ACACCACTGT AGACATCAAA CTCAAGCAAT GGACTGATAA ACAACTTCCT
    CATAAAGCAC TAGAGGTTGC TTGGGAGACT TTGCAGGAAG AGTTTGCCCG CTTCATGGCC
    GAATACAAAG GGAAAGACCA GGATGACATT TTTGACAAGC TGAAGGAAGC TGTCAAGGAC
    GAGAGCATCA AACGACACAA GTGGAATGAA AGAGCCATGG ACAGCTTGAG AGTGATTCAA
    CACAACGCTC TGGAGGATCG TTCAATCACA GACAAGCCCC AGTGGGATGC CGCTATACAG
    TTCATGGAGG AAACGCTGCA GTCTCGTCTT AAAGACACTG AGTCAGTAAT AGCTGATATG
    GTGGGGCCAG ACTGGAAGCA GAGGTGGATG AGCTGGAAAA ACCGAACACC AGAGCAGCAC
    ACCCGGAATG AAACCAAAAA TGAGCTGGAG CGTCTGCTGA AACTGCATGA GGATCACACA
    GCATATTTGG CCAATGATGA GGTCACTACA GTGCGCAAAA ACCTGGAGGC CCGAGGGGTG
    GAGGTGGACC CTGTATTGAT TAAGGACACA TGGCATCAGC TCTTTCGACG TCACTTCCTA
    CAGAAGGCAC TACTACATTG CAACCTCTGC AGACGAGGTT TCTACTACTA TCAGAGGCAC
    TTCGTAGACT CTGAGCTTGA GTGCAATGAT GTGGTTCTGT TCTGGAGGAT TCAGAGGATG
    TTGGGCATCA CTGCCAATAC TCTTCGACAA CAGCTCACTA ACACTGAGGT ACGGCGTTTG
    GAGAAGAACG TTAAAGAAGT ATTAGAAGAC TTTGGAGAGG ACAATGAGAA AAAAGTCCAG
    CTGATCACAG GCAGGAGGGT TCAACTGGCA GAAGACCTCA AAAAAGTTCG CGAAATTCAG
    GAGAAACTTG AAGCCTTCAT TGAAGCTCTT CACAAAGAGA AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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