ORF » Species Summary » Danio rerio » spef2 cDNA ORF clone

spef2 cDNA ORF clone, Danio rerio(zebrafish)

The following spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa41928 XM_009301185.3
Latest version!		 Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa33525 NM_001128232.1
Latest version!		 Danio rerio sperm flagellar 2 (spef2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa41924 XM_009301181.3
Latest version!		 Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41925 XM_009301182.3
Latest version!		 Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41926 XM_009301183.3
Latest version!		 Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41927 XM_009301184.3
Latest version!		 Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa41925 XM_009301182.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41926 XM_009301183.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41928 XM_009301185.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa41924 XM_009301181.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa41927 XM_009301184.2 Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    spef2 ( XM_009301185.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299460.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa41928
    Clone ID Related Accession (Same CDS sequence) XM_009301185.2 , XM_009301185.3
    Accession Version XM_009301185.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC 
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG 
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG 
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT 
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG 
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA 
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC 
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC 
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA 
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT 
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC 
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG 
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG 
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG 
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT 
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA 
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG 
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG 
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG 
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT 
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC 
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT 
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT 
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT 
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA 
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA 
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT 
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA 
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA 
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT 
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC 
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA 
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG 
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG 
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC 
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG 
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA 
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA 
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA 
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT 
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT 
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA 
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG 
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA 
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC 
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC 
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA 
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG 
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG 
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA 
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG 
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT 
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA 
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA 
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299460.1
    CDS38..3361
    Translation

    Target ORF information:

    RefSeq Version XM_009301185.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301185.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC 
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG 
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG 
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT 
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG 
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA 
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC 
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC 
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA 
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT 
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC 
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG 
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG 
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG 
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT 
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA 
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG 
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG 
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG 
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT 
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC 
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT 
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT 
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT 
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA 
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA 
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT 
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA 
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA 
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT 
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC 
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA 
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG 
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG 
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC 
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG 
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA 
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA 
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA 
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT 
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT 
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA 
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG 
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA 
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC 
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC 
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA 
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG 
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG 
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA 
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG 
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT 
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA 
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA 
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301185.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299460.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID ODa41928
    Clone ID Related Accession (Same CDS sequence) XM_009301185.2 , XM_009301185.3
    Accession Version XM_009301185.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC 
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG 
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG 
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT 
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG 
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA 
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC 
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC 
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA 
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT 
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC 
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG 
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG 
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG 
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT 
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA 
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG 
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG 
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG 
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT 
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC 
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT 
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT 
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT 
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA 
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA 
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT 
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA 
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA 
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT 
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC 
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA 
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG 
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG 
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC 
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG 
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA 
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA 
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA 
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT 
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT 
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA 
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG 
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA 
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC 
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC 
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA 
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG 
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG 
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA 
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG 
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT 
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA 
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA 
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299460.1
    CDS36..3359
    Translation

    Target ORF information:

    RefSeq Version XM_009301185.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301185.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAATGATC CCAAACCTAT CCCATATTTT AACTCCAACC ATATAAAGAG CTTGGAGAAA 
    AATGTTTCTC AGACAGAACA TACTACAGAA CAGCTTCAGA AAGATGAGCA GGAGACATCC 
    TCTCCTCTAG AATCAGGACC ACAGCCTCAT GAGGCCTCAT CTGGGCTTCA TGAAAAAGAG 
    GACAAAGAAG AATCAAAGGC AATCCCAAAG TGGTCATCAC GAGCACAGCA CGGTGCAGCG 
    GTAGAGAAAG TTCTGAGGAC AGGCCAAGCT GTTCCTGATC AGCTTCTGGT GGACATTATT 
    GTGGATGCTA TCAGGAACAT TCCTGCAGAT AGTGGCTGGA TTCTTGATGG CTTCCCGGTG 
    GACATCAGTC AGGCTCAGAT GCTTGAGAAA GCTCTAAACA TGACTGAACT TGATGGAACA 
    AAAACACAAA ATAATTTGTC CACAAACAAA AACGGCCCTA AAGATGCGGC TCCTCCATCC 
    CCTGCTCTAG ACCTAGTGGT GCTGTTAGAC GTTTCAGATG AGCAGGTTCT GGAGCGAGCC 
    ACAACTCAGC CTCCTGAGGG AACACAGATA GTGAATGGAG AAAGCATAAA GCAGGAGAAA 
    GGCAGCAGTG ATGCTGCGGA GGAAAACACA AGCATGCCAG ATCTGAACAC ACAAGATCCT 
    GAATCCTTGT CTGACGACAA AAGCCTGGGG AGGACACAAA TACAGTTCAG AATCAGTGGC 
    TTTCATCAAA CATGGCCAAA ACTGGAGAAG TGGTTTGGTG ATCAGCAGAA TATTCTGGTG 
    AAAGTCACAG CAGGTGTGGA CAAGGACACT CTCTTCAGTA ACGTGGAGAC TATTCTGAGG 
    GACACTATTG AGTCTGTTGA AAAAGGAGCA GCTCTGGCTG ACTCCAGGAG AGCTTCAGTG 
    ACCTCCACAC CTGAACAGAC CCGTCCAGAA AGTGCCTGCT CTTCCAAATC TTCCCGAGCT 
    ACCCCAAAAT CTGCTAGAAG AAGCTTTGCG GAGGAACCTT TACCCAAGGA GTTGGCAGAA 
    TATCTTGTGC CATACTGGGA GAACTTCTGT AGTTCATATG TGACTAATGT CAAAACAGTG 
    ATGCAGAATC TCCGTGGTGA ACGTGAGCTC ATCATTCATC ACCTTTACAA CATCCGGGAG 
    AACTTCAGGC AGTATCTACA GAAGCCGGAT CTGAAGCAGG AGTTTGTTAG CTCATGGCAG 
    CGAGATTATA ACAGTGTTCC TGATAACATC AGAGAGGACG AGGAGACCAA AGGAGAGCTT 
    CATCAAAGGC TGGATGACTT GCGTGAGCGG CTATGGGACA TCAGTGATAA GCGTAAAGAC 
    GAGGCAGAGC AGGAGAAAGC AGGCGTCATT CATGATGGCT GGTTAGAGGA TCACACCGCT 
    GTACTAATCA ACAATTATTC TGCATTGATG CAGATAGAAG TGGGCCGATT TCAGGACTGT 
    GTGTGTCTGC TGAAAGATTA TTATTCAACG ATGGGTAAAA CAGCCTTTGA ATCCAAATGT 
    GACTTTGCTC GTGTCCCATT GCTTGACATC ACCGCTGATC AGCATGTCGA CGAGTCGAAA 
    ATCTCTGAAT CTGCTCCGTC AGAGAAATCT GTAAAGAATG CTGAGGAAAA AGACTCTGAA 
    ATTGTGGACA GGATGAAAAC TAAAGTGTTT CCACTGGTTT CCTGCAGATC TCCAACTTCT 
    GAGATCCTCA AACAGCCTTT GCATCAGGCT GATGAGAACC TGCTGCAGGA GTTCTTCCAA 
    GCAGCAATCG CCATCTTCAG GAGCATGGTG TCGGCAGAGA CGCGGCAGTG GGATGAAGAA 
    GAAGTTGATG AAGACCAGAA GCAGATGGAG GCACAGAAAG CTCAAACTTT GACCTCTGCT 
    ACAGACGTAA GGAAAGCAGG AAAACAAAAA GGTGCTCCAC CTCCTCCTCC TCCAGAGCCC 
    AGTCCTCAAC CTGCTGCAAG GAACCCAGAG GAGCTCAGGA AGAGAGCGGA GAAGAAACGA 
    TTCAAACAGG AGCTCTCAGC TGCACTTAAA CCTGAAGAAA ATGCAGTGAA GCAGCGTTTG 
    GAGCTAGTGA AGGCTCATGG GCTGAGAACA GTCATCAGTC TGCAGCACAA AGCAGAACAG 
    GTCTATAGGA ACATGGAGGA GTGGATGAAA GCCCGCTATC TGGCTGAAAT GAAAAGTATC 
    GACCAGCTGA CAGATTTGGT GAGGCAGCAC ATTGAGACTG GAGTTCAGAT CCGTCATGAG 
    CTGGTTCTGC AGAGCACTGA TTTCTGTGTG GATGGAGACA CCCGTGTGCT GTTGAGTCCA 
    GAGCTTACTC CGCGCCGATC CCTGCTGGAG CAGCCTAAAA ACAGCACACT CACAGTACAA 
    CAGCTGCACG TCCTCTGCAC ACAGTTGCGC AAGATCGCCC CTAAAGGTCT GATGTGTAGA 
    AGTGAGCTGA CTGAAGCTCT ACACGAATTA ACCTCTGCTC ACATGGGTAC TGATGTCCTT 
    CCTGAGGCCT GGATGCACAT CACTCCTTCA CAGGTTGAGG AGCTGGTGTG TATTCTAGCT 
    CAGGACTCTG AGATGTTGGA CTGGCGTCAG TTTCTGCTCA GTGCTGCTCT GCCGTGGCCA 
    TTTCCCACTC AGACTCAGCT GATAAAAACC CTCCAGCGCT ACAGAGACAT CGACACCGCG 
    GAAACCGGAC TCATCACACA AGAACAATAT GAACAGGTTG AATTATGGTT TCCCAGTGAA 
    CAAGATCCTT CTGTCCCTGA TGACCCAACT GAACCCGTGC CCTATGACAG ATTGGCCAAC 
    CTTAAAAAGT TTTTCTTCAG TCTGTTTGCG AGCACACACT CCTCACCACT GCTGCTGGAC 
    TATCTGATGA TGCTGCTTCA TTTCTGCTGC CATCCTGAAG CAGCTCAGGG CTTCACCAGA 
    GCGCTCGGCC TCGTCACCGG ACACACACTG CACTACAAAC ACACCACCCC ACTCACACAG 
    TCTGTGCCGT GTCTGGAGGG CGCTGGAGTT GTGGATGATG CTGGTGCTGA TAATGAAGAG 
    GAGCAGCACA GAGGGGTGGA GTCTGAGGAA GTGGGCGTGT CTGTTGATGA TGTGCTCAGA 
    GTTTTGAGTC ATGGAGAGGA TCTCGTCTTA CATCTAAACC GCTTCCAGCT TGTCCACAGG 
    AGCAGAGATG AACTCCGAGA GGAGCTTCTG AAGGTGTTTA AGGACTTGGG TTTCAGTGGT 
    GAAGAGAAGA TTCCTTTCTC CACACTATCT CAGCATCCCT TTCTGCAGGA CCTGATAGAA 
    GGATCCGCCT ATTATCTGCT CCATGACATT CACAAAATTT TCCAGACAAA ACAAACTGAA 
    GATTCGAGAA GTTTCTCAAC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( NM_001128232.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121704.1 Danio rerio sperm flagellar 2 (spef2), mRNA.
    Price & Availability↑
    CloneID ODa33525
    Clone ID Related Accession (Same CDS sequence) NM_001128232.1
    Accession Version NM_001128232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-05
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR450726.6. On Jul 31, 2008 this sequence version replaced XM_694196.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAATGATG CAGAATCTCC GTGGTGAACG TGAGCTCATC 
    ATTCATCACC TTTACAACAT CCGGGAGAAC TTCAGGCAGT ATCTACAGAA GCCGGATCTG 
    AAGCAGGAGT TTGTTAGCTC ATGGCAGCGA GATTATAACA GTGTTCCTGA TAACATCAGA 
    GAGGACGAGG AGACCAAAGG AGAGCTTCAT CAAAGGCTGG ATGACTTGCG TGAGCGGCTA 
    TGGGACATCA GTGATAAGCG TAAAGACGAG GCAGAGCAGG AGAAAGCAGG CGTCATTCAT 
    GATGGCTGGT TAGAGGATCA CACCGCTGTA CTAATCAACA ATTATTCTGC ATTGATGCAG 
    ATAGAAGTGG GCCGATTTCA GGACTGTGTG TGTCTGCTGA AAGATTATTA TTCAACGATG 
    GGTAAAACAG CCTTTGAATC CAAATGTGAC TTTGCTCGTG TCCCATTGCT TGACATCACC 
    GCTGATCAGC ATGTCGACGA GTCGAAAATC TCTGAATCTG CTCCGTCAGA GAAATCTGTA 
    AAGAATGCTG AGGAAAAAGA CTCTGAAATT GTGGACAGGA TGAAAACTAA AGTATCTCCA 
    ACTTCTGAGA TCCTCAAACA GCCTTTGCAT CAGGCTGATG AGAACCTGCT GCAGGAGTTC 
    TTCCAAGCAG CAATCGCCAT CTTCAGGAGC ATGGTGTCGG CAGAGACGCG GCAGTGGGAT 
    GAAGAAGAAG TTGATGAAGA CCAGAAGCAG ATGGAGGCAC AGAAAGCTCA AACTTTGACC 
    TCTGCTACAG ACGTAAGGAA AGCAGGAAAA CAAAAAGGTG CTCCACCTCC TCCTCCTCCA 
    GAGCCCAGTC CTCAACCTGC TGCAAGGAAC CCAGAGGAGC TCAGGAAGAG AGCGGAGAAG 
    AAACGATTCA AACAGGAGCT CTCAGCTGCA CTTAAACCTG AAGTGAAGGC TCATGGGCTG 
    AGAACAGTCA TCAGTCTGCA GCACAAAGCA GAACAGGTCT ATAGGAACAT GGAGGAGTGG 
    ATGAAAGCCC GCTATCTGGC TGAAATGAAA AGTATCGACC AGCTGACAGA TTTGGTGAGG 
    CAGCACATTG AGACTGGAGT TCAGATCCGT CATGAGCTGG TTCTGCAGAG CACTGATTTC 
    TGTGTGGATG GAGACACCCG TGTGCTGTTG AGTCCAGAGC TTACTCCGCG CCGATCCCTG 
    CTGGAGCAGC CTAAAAACAG CACACTCACA GTACAACAGC TGCACGTCCT CTGCACACAG 
    TTGCGCAAGA TCGCCCCTAA AGGTCTGATG TGTAGAAGTG AGCTGACTGA AGCTCTACAC 
    GAATTAACCT CTGCTCACAT GGGTACTGAT GTCCTTCCTG AGGCCTGGAT GCACATCACT 
    CCTTCACAGG TTGAGGAGCT GGTGTGTATT CTAGCTCAGG ACTCTGAGAT GTTGGACTGG 
    CGTCAGTTTC TGCTCAGTGC TGCTCTGCCG TGGCCATTTC CCACTCAGAC TCAGCTGATA 
    AAAACCCTCC AGCGCTACAG AGACATCGAC ACCGCGGAAA CCGGACTCAT CACACAAGAA 
    CAATATGAAC AGGTTGAATT ATGGTTTCCC AGTGAACAAG ATCCTTCTGT CCCTGATGAC 
    CCAACTGAAC CCGTGCCCTA TGACAGATTG GCCAACCTTA AAAAGTTTTT CTTCAGTCTG 
    TTTGCGAGCA CACACTCCTC ACCACTGCTG CTGGACTATC TGATGATGCT GCTTCATTTC 
    TGCTGCCATC CTGAAGCAGC TCAGGGCTTC ACCAGAGCGC TCGGCCTCGT CACCGGACAC 
    ACACTGCACT ACAAACACAC CACCCCACTC ACACAGTCTG TGCCGTGTCT GGAGGGCGCT 
    GGAGTTGTGG ATGATGCTGG TGCTGATAAT GAAGAGGAGC AGCACAGAGG GGTGGAGTCT 
    GAGGAAGTGG GCGTGTCTGT TGATGATGTG CTCAGAGTTT TGAGTCATGG AGAGGATCTC 
    GTCTTACATC TAAACCGCTT CCAGCTTGTC CACAGGAGCA GAGATGAACT CCGAGAGGAG 
    CTTCTGAAGG TGTTTAAGGA CTTGGGTTTC AGTGGTGAAG AGAAGATTCC TTTCTCCACA 
    CTATCTCAGC ATCCCTTTCT GCAGGACCTG ATAGAAGGAT CCGCCTATTA TCTGCTCCAT 
    GACATTCACA AAATTTTCCA GACAAAACAA ACTGAAGATT CGAGAAGTTT CTCAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001121704.1
    CDS112..5031
    Translation

    Target ORF information:

    RefSeq Version NM_001128232.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001128232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAATGATG CAGAATCTCC GTGGTGAACG TGAGCTCATC 
    ATTCATCACC TTTACAACAT CCGGGAGAAC TTCAGGCAGT ATCTACAGAA GCCGGATCTG 
    AAGCAGGAGT TTGTTAGCTC ATGGCAGCGA GATTATAACA GTGTTCCTGA TAACATCAGA 
    GAGGACGAGG AGACCAAAGG AGAGCTTCAT CAAAGGCTGG ATGACTTGCG TGAGCGGCTA 
    TGGGACATCA GTGATAAGCG TAAAGACGAG GCAGAGCAGG AGAAAGCAGG CGTCATTCAT 
    GATGGCTGGT TAGAGGATCA CACCGCTGTA CTAATCAACA ATTATTCTGC ATTGATGCAG 
    ATAGAAGTGG GCCGATTTCA GGACTGTGTG TGTCTGCTGA AAGATTATTA TTCAACGATG 
    GGTAAAACAG CCTTTGAATC CAAATGTGAC TTTGCTCGTG TCCCATTGCT TGACATCACC 
    GCTGATCAGC ATGTCGACGA GTCGAAAATC TCTGAATCTG CTCCGTCAGA GAAATCTGTA 
    AAGAATGCTG AGGAAAAAGA CTCTGAAATT GTGGACAGGA TGAAAACTAA AGTATCTCCA 
    ACTTCTGAGA TCCTCAAACA GCCTTTGCAT CAGGCTGATG AGAACCTGCT GCAGGAGTTC 
    TTCCAAGCAG CAATCGCCAT CTTCAGGAGC ATGGTGTCGG CAGAGACGCG GCAGTGGGAT 
    GAAGAAGAAG TTGATGAAGA CCAGAAGCAG ATGGAGGCAC AGAAAGCTCA AACTTTGACC 
    TCTGCTACAG ACGTAAGGAA AGCAGGAAAA CAAAAAGGTG CTCCACCTCC TCCTCCTCCA 
    GAGCCCAGTC CTCAACCTGC TGCAAGGAAC CCAGAGGAGC TCAGGAAGAG AGCGGAGAAG 
    AAACGATTCA AACAGGAGCT CTCAGCTGCA CTTAAACCTG AAGTGAAGGC TCATGGGCTG 
    AGAACAGTCA TCAGTCTGCA GCACAAAGCA GAACAGGTCT ATAGGAACAT GGAGGAGTGG 
    ATGAAAGCCC GCTATCTGGC TGAAATGAAA AGTATCGACC AGCTGACAGA TTTGGTGAGG 
    CAGCACATTG AGACTGGAGT TCAGATCCGT CATGAGCTGG TTCTGCAGAG CACTGATTTC 
    TGTGTGGATG GAGACACCCG TGTGCTGTTG AGTCCAGAGC TTACTCCGCG CCGATCCCTG 
    CTGGAGCAGC CTAAAAACAG CACACTCACA GTACAACAGC TGCACGTCCT CTGCACACAG 
    TTGCGCAAGA TCGCCCCTAA AGGTCTGATG TGTAGAAGTG AGCTGACTGA AGCTCTACAC 
    GAATTAACCT CTGCTCACAT GGGTACTGAT GTCCTTCCTG AGGCCTGGAT GCACATCACT 
    CCTTCACAGG TTGAGGAGCT GGTGTGTATT CTAGCTCAGG ACTCTGAGAT GTTGGACTGG 
    CGTCAGTTTC TGCTCAGTGC TGCTCTGCCG TGGCCATTTC CCACTCAGAC TCAGCTGATA 
    AAAACCCTCC AGCGCTACAG AGACATCGAC ACCGCGGAAA CCGGACTCAT CACACAAGAA 
    CAATATGAAC AGGTTGAATT ATGGTTTCCC AGTGAACAAG ATCCTTCTGT CCCTGATGAC 
    CCAACTGAAC CCGTGCCCTA TGACAGATTG GCCAACCTTA AAAAGTTTTT CTTCAGTCTG 
    TTTGCGAGCA CACACTCCTC ACCACTGCTG CTGGACTATC TGATGATGCT GCTTCATTTC 
    TGCTGCCATC CTGAAGCAGC TCAGGGCTTC ACCAGAGCGC TCGGCCTCGT CACCGGACAC 
    ACACTGCACT ACAAACACAC CACCCCACTC ACACAGTCTG TGCCGTGTCT GGAGGGCGCT 
    GGAGTTGTGG ATGATGCTGG TGCTGATAAT GAAGAGGAGC AGCACAGAGG GGTGGAGTCT 
    GAGGAAGTGG GCGTGTCTGT TGATGATGTG CTCAGAGTTT TGAGTCATGG AGAGGATCTC 
    GTCTTACATC TAAACCGCTT CCAGCTTGTC CACAGGAGCA GAGATGAACT CCGAGAGGAG 
    CTTCTGAAGG TGTTTAAGGA CTTGGGTTTC AGTGGTGAAG AGAAGATTCC TTTCTCCACA 
    CTATCTCAGC ATCCCTTTCT GCAGGACCTG ATAGAAGGAT CCGCCTATTA TCTGCTCCAT 
    GACATTCACA AAATTTTCCA GACAAAACAA ACTGAAGATT CGAGAAGTTT CTCAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301181.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299456.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa41924
    Clone ID Related Accession (Same CDS sequence) XM_009301181.3 , XM_009301181.2
    Accession Version XM_009301181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299456.1
    CDS125..5242
    Translation

    Target ORF information:

    RefSeq Version XM_009301181.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301181.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299456.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa41924
    Clone ID Related Accession (Same CDS sequence) XM_009301181.3 , XM_009301181.2
    Accession Version XM_009301181.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299456.1
    CDS130..5247
    Translation

    Target ORF information:

    RefSeq Version XM_009301181.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301182.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299457.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa41925
    Clone ID Related Accession (Same CDS sequence) XM_009301182.3 , XM_009301182.2
    Accession Version XM_009301182.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT 
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA 
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA 
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA 
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT 
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT 
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA 
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA 
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG 
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT 
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG 
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA 
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA 
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT 
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA 
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA 
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG 
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT 
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG 
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT 
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG 
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG 
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC 
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG 
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT 
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA 
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC 
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC 
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC 
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT 
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA 
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA 
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG 
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG 
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG 
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG 
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG 
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT 
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC 
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG 
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC 
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT 
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT 
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA 
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT 
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG 
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG 
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC 
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG 
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC 
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT 
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT 
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG 
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC 
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA 
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299457.1
    CDS125..5239
    Translation

    Target ORF information:

    RefSeq Version XM_009301182.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301182.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT 
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA 
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA 
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA 
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT 
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT 
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA 
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA 
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG 
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT 
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG 
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA 
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA 
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT 
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA 
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA 
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG 
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT 
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG 
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT 
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG 
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG 
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC 
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG 
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT 
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA 
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC 
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC 
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC 
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT 
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA 
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA 
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG 
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG 
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG 
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG 
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG 
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT 
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC 
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG 
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC 
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT 
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT 
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA 
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT 
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG 
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG 
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC 
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG 
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC 
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT 
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT 
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG 
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC 
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA 
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301182.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299457.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa41925
    Clone ID Related Accession (Same CDS sequence) XM_009301182.3 , XM_009301182.2
    Accession Version XM_009301182.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301182.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT 
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA 
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA 
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA 
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT 
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT 
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA 
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA 
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG 
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT 
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG 
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA 
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA 
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT 
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA 
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA 
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG 
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT 
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG 
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT 
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG 
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG 
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC 
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG 
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT 
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA 
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC 
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC 
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC 
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT 
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA 
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA 
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG 
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG 
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG 
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG 
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG 
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT 
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC 
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG 
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC 
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT 
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT 
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA 
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT 
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG 
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG 
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC 
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG 
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC 
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT 
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT 
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG 
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC 
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA 
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299457.1
    CDS130..5244
    Translation

    Target ORF information:

    RefSeq Version XM_009301182.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301182.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA GCTTGGAGAA AAATGTTTCT 
    CAGACAGAAC ATACTACAGA ACAGCTTCAG AAAGATGAGC AGGAGACATC CTCTCCTCTA 
    GAATCAGGAC CACAGCCTCA TGAGGCCTCA TCTGGGCTTC ATGAAAAAGA GGACAAAGAA 
    GAATCAAAGG CAATCCCAAA GTGGTCATCA CGAGCACAGC ACGGTGCAGC GGTAGAGAAA 
    GTTCTGAGGA CAGGCCAAGC TGTTCCTGAT CAGCTTCTGG TGGACATTAT TGTGGATGCT 
    ATCAGGAACA TTCCTGCAGA TAGTGGCTGG ATTCTTGATG GCTTCCCGGT GGACATCAGT 
    CAGGCTCAGA TGCTTGAGAA AGCTCTAAAC ATGACTGAAC TTGATGGAAC AAAAACACAA 
    AATAATTTGT CCACAAACAA AAACGGCCCT AAAGATGCGG CTCCTCCATC CCCTGCTCTA 
    GACCTAGTGG TGCTGTTAGA CGTTTCAGAT GAGCAGGTTC TGGAGCGAGC CACAACTCAG 
    CCTCCTGAGG GAACACAGAT AGTGAATGGA GAAAGCATAA AGCAGGAGAA AGGCAGCAGT 
    GATGCTGCGG AGGAAAACAC AAGCATGCCA GATCTGAACA CACAAGATCC TGAATCCTTG 
    TCTGACGACA AAAGCCTGGG GAGGACACAA ATACAGTTCA GAATCAGTGG CTTTCATCAA 
    ACATGGCCAA AACTGGAGAA GTGGTTTGGT GATCAGCAGA ATATTCTGGT GAAAGTCACA 
    GCAGGTGTGG ACAAGGACAC TCTCTTCAGT AACGTGGAGA CTATTCTGAG GGACACTATT 
    GAGTCTGTTG AAAAAGGAGC AGCTCTGGCT GACTCCAGGA GAGCTTCAGT GACCTCCACA 
    CCTGAACAGA CCCGTCCAGA AAGTGCCTGC TCTTCCAAAT CTTCCCGAGC TACCCCAAAA 
    TCTGCTAGAA GAAGCTTTGC GGAGGAACCT TTACCCAAGG AGTTGGCAGA ATATCTTGTG 
    CCATACTGGG AGAACTTCTG TAGTTCATAT GTGACTAATG TCAAAACAGT GATGCAGAAT 
    CTCCGTGGTG AACGTGAGCT CATCATTCAT CACCTTTACA ACATCCGGGA GAACTTCAGG 
    CAGTATCTAC AGAAGCCGGA TCTGAAGCAG GAGTTTGTTA GCTCATGGCA GCGAGATTAT 
    AACAGTGTTC CTGATAACAT CAGAGAGGAC GAGGAGACCA AAGGAGAGCT TCATCAAAGG 
    CTGGATGACT TGCGTGAGCG GCTATGGGAC ATCAGTGATA AGCGTAAAGA CGAGGCAGAG 
    CAGGAGAAAG CAGGCGTCAT TCATGATGGC TGGTTAGAGG ATCACACCGC TGTACTAATC 
    AACAATTATT CTGCATTGAT GCAGATAGAA GTGGGCCGAT TTCAGGACTG TGTGTGTCTG 
    CTGAAAGATT ATTATTCAAC GATGGGTAAA ACAGCCTTTG AATCCAAATG TGACTTTGCT 
    CGTGTCCCAT TGCTTGACAT CACCGCTGAT CAGCATGTCG ACGAGTCGAA AATCTCTGAA 
    TCTGCTCCGT CAGAGAAATC TGTAAAGAAT GCTGAGGAAA AAGACTCTGA AATTGTGGAC 
    AGGATGAAAA CTAAAGTGTT TCCACTGGTT TCCTGCAGAT CTCCAACTTC TGAGATCCTC 
    AAACAGCCTT TGCATCAGGC TGATGAGAAC CTGCTGCAGG AGTTCTTCCA AGCAGCAATC 
    GCCATCTTCA GGAGCATGGT GTCGGCAGAG ACGCGGCAGT GGGATGAAGA AGAAGTTGAT 
    GAAGACCAGA AGCAGATGGA GGCACAGAAA GCTCAAACTT TGACCTCTGC TACAGACGTA 
    AGGAAAGCAG GAAAACAAAA AGGTGCTCCA CCTCCTCCTC CTCCAGAGCC CAGTCCTCAA 
    CCTGCTGCAA GGAACCCAGA GGAGCTCAGG AAGAGAGCGG AGAAGAAACG ATTCAAACAG 
    GAGCTCTCAG CTGCACTTAA ACCTGAAGAA AATGCAGTGA AGCAGCGTTT GGAGCTAGTG 
    AAGGCTCATG GGCTGAGAAC AGTCATCAGT CTGCAGCACA AAGCAGAACA GGTCTATAGG 
    AACATGGAGG AGTGGATGAA AGCCCGCTAT CTGGCTGAAA TGAAAAGTAT CGACCAGCTG 
    ACAGATTTGG TGAGGCAGCA CATTGAGACT GGAGTTCAGA TCCGTCATGA GCTGGTTCTG 
    CAGAGCACTG ATTTCTGTGT GGATGGAGAC ACCCGTGTGC TGTTGAGTCC AGAGCTTACT 
    CCGCGCCGAT CCCTGCTGGA GCAGCCTAAA AACAGCACAC TCACAGTACA ACAGCTGCAC 
    GTCCTCTGCA CACAGTTGCG CAAGATCGCC CCTAAAGGTC TGATGTGTAG AAGTGAGCTG 
    ACTGAAGCTC TACACGAATT AACCTCTGCT CACATGGGTA CTGATGTCCT TCCTGAGGCC 
    TGGATGCACA TCACTCCTTC ACAGGTTGAG GAGCTGGTGT GTATTCTAGC TCAGGACTCT 
    GAGATGTTGG ACTGGCGTCA GTTTCTGCTC AGTGCTGCTC TGCCGTGGCC ATTTCCCACT 
    CAGACTCAGC TGATAAAAAC CCTCCAGCGC TACAGAGACA TCGACACCGC GGAAACCGGA 
    CTCATCACAC AAGAACAATA TGAACAGGTT GAATTATGGT TTCCCAGTGA ACAAGATCCT 
    TCTGTCCCTG ATGACCCAAC TGAACCCGTG CCCTATGACA GATTGGCCAA CCTTAAAAAG 
    TTTTTCTTCA GTCTGTTTGC GAGCACACAC TCCTCACCAC TGCTGCTGGA CTATCTGATG 
    ATGCTGCTTC ATTTCTGCTG CCATCCTGAA GCAGCTCAGG GCTTCACCAG AGCGCTCGGC 
    CTCGTCACCG GACACACACT GCACTACAAA CACACCACCC CACTCACACA GTCTGTGCCG 
    TGTCTGGAGG GCGCTGGAGT TGTGGATGAT GCTGGTGCTG ATAATGAAGA GGAGCAGCAC 
    AGAGGGGTGG AGTCTGAGGA AGTGGGCGTG TCTGTTGATG ATGTGCTCAG AGTTTTGAGT 
    CATGGAGAGG ATCTCGTCTT ACATCTAAAC CGCTTCCAGC TTGTCCACAG GAGCAGAGAT 
    GAACTCCGAG AGGAGCTTCT GAAGGTGTTT AAGGACTTGG GTTTCAGTGG TGAAGAGAAG 
    ATTCCTTTCT CCACACTATC TCAGCATCCC TTTCTGCAGG ACCTGATAGA AGGATCCGCC 
    TATTATCTGC TCCATGACAT TCACAAAATT TTCCAGACAA AACAAACTGA AGATTCGAGA 
    AGTTTCTCAA CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301183.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299458.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa41926
    Clone ID Related Accession (Same CDS sequence) XM_009301183.2 , XM_009301183.3
    Accession Version XM_009301183.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT 
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT 
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA 
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC 
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG 
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC 
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG 
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG 
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC 
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG 
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT 
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT 
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG 
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA 
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC 
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA 
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG 
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT 
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG 
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG 
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC 
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA 
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG 
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG 
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC 
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT 
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG 
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG 
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG 
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC 
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT 
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT 
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG 
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC 
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT 
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA 
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT 
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA 
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT 
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT 
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT 
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299458.1
    CDS130..5241
    Translation

    Target ORF information:

    RefSeq Version XM_009301183.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT 
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT 
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA 
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC 
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG 
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC 
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG 
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG 
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC 
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG 
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT 
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT 
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG 
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA 
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC 
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA 
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG 
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT 
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG 
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG 
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC 
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA 
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG 
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG 
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC 
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT 
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG 
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG 
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG 
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC 
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT 
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT 
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG 
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC 
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT 
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA 
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT 
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA 
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT 
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT 
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT 
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301183.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299458.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID ODa41926
    Clone ID Related Accession (Same CDS sequence) XM_009301183.2 , XM_009301183.3
    Accession Version XM_009301183.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301183.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT 
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT 
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA 
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC 
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG 
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC 
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG 
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG 
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC 
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG 
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT 
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT 
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG 
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA 
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC 
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA 
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG 
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT 
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG 
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG 
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC 
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA 
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG 
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG 
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC 
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT 
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG 
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG 
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG 
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC 
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT 
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT 
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG 
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC 
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT 
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA 
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT 
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA 
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT 
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT 
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT 
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299458.1
    CDS125..5236
    Translation

    Target ORF information:

    RefSeq Version XM_009301183.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301183.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGTCAGACA TTATCTGTCA GTGGCTGAAT TCAGAGCTCC GGCTGTCAAA AGTCGTCGAG 
    CCGCATTCAT TTGCCAGGGA TTTTTCAAAC GGTTATCTGA TAGGAGAGAT CCTCTACAGA 
    TATGAGCTAC AAGACGATTT CCATCTGTTC TCAAAACAAA ACACATCGAA TGCCAAGCTA 
    AATAACTTCA CTCGCATTGA GCCCACCCTG CAGCTGCTGA CCGTGCCCTT TGATCTGACC 
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGC TCTGGCTGAC TCCAGGAGAG CTTCAGTGAC CTCCACACCT 
    GAACAGACCC GTCCAGAAAG TGCCTGCTCT TCCAAATCTT CCCGAGCTAC CCCAAAATCT 
    GCTAGAAGAA GCTTTGCGGA GGAACCTTTA CCCAAGGAGT TGGCAGAATA TCTTGTGCCA 
    TACTGGGAGA ACTTCTGTAG TTCATATGTG ACTAATGTCA AAACAGTGAT GCAGAATCTC 
    CGTGGTGAAC GTGAGCTCAT CATTCATCAC CTTTACAACA TCCGGGAGAA CTTCAGGCAG 
    TATCTACAGA AGCCGGATCT GAAGCAGGAG TTTGTTAGCT CATGGCAGCG AGATTATAAC 
    AGTGTTCCTG ATAACATCAG AGAGGACGAG GAGACCAAAG GAGAGCTTCA TCAAAGGCTG 
    GATGACTTGC GTGAGCGGCT ATGGGACATC AGTGATAAGC GTAAAGACGA GGCAGAGCAG 
    GAGAAAGCAG GCGTCATTCA TGATGGCTGG TTAGAGGATC ACACCGCTGT ACTAATCAAC 
    AATTATTCTG CATTGATGCA GATAGAAGTG GGCCGATTTC AGGACTGTGT GTGTCTGCTG 
    AAAGATTATT ATTCAACGAT GGGTAAAACA GCCTTTGAAT CCAAATGTGA CTTTGCTCGT 
    GTCCCATTGC TTGACATCAC CGCTGATCAG CATGTCGACG AGTCGAAAAT CTCTGAATCT 
    GCTCCGTCAG AGAAATCTGT AAAGAATGCT GAGGAAAAAG ACTCTGAAAT TGTGGACAGG 
    ATGAAAACTA AAGTGTTTCC ACTGGTTTCC TGCAGATCTC CAACTTCTGA GATCCTCAAA 
    CAGCCTTTGC ATCAGGCTGA TGAGAACCTG CTGCAGGAGT TCTTCCAAGC AGCAATCGCC 
    ATCTTCAGGA GCATGGTGTC GGCAGAGACG CGGCAGTGGG ATGAAGAAGA AGTTGATGAA 
    GACCAGAAGC AGATGGAGGC ACAGAAAGCT CAAACTTTGA CCTCTGCTAC AGACGTAAGG 
    AAAGCAGGAA AACAAAAAGG TGCTCCACCT CCTCCTCCTC CAGAGCCCAG TCCTCAACCT 
    GCTGCAAGGA ACCCAGAGGA GCTCAGGAAG AGAGCGGAGA AGAAACGATT CAAACAGGAG 
    CTCTCAGCTG CACTTAAACC TGAAGAAAAT GCAGTGAAGC AGCGTTTGGA GCTAGTGAAG 
    GCTCATGGGC TGAGAACAGT CATCAGTCTG CAGCACAAAG CAGAACAGGT CTATAGGAAC 
    ATGGAGGAGT GGATGAAAGC CCGCTATCTG GCTGAAATGA AAAGTATCGA CCAGCTGACA 
    GATTTGGTGA GGCAGCACAT TGAGACTGGA GTTCAGATCC GTCATGAGCT GGTTCTGCAG 
    AGCACTGATT TCTGTGTGGA TGGAGACACC CGTGTGCTGT TGAGTCCAGA GCTTACTCCG 
    CGCCGATCCC TGCTGGAGCA GCCTAAAAAC AGCACACTCA CAGTACAACA GCTGCACGTC 
    CTCTGCACAC AGTTGCGCAA GATCGCCCCT AAAGGTCTGA TGTGTAGAAG TGAGCTGACT 
    GAAGCTCTAC ACGAATTAAC CTCTGCTCAC ATGGGTACTG ATGTCCTTCC TGAGGCCTGG 
    ATGCACATCA CTCCTTCACA GGTTGAGGAG CTGGTGTGTA TTCTAGCTCA GGACTCTGAG 
    ATGTTGGACT GGCGTCAGTT TCTGCTCAGT GCTGCTCTGC CGTGGCCATT TCCCACTCAG 
    ACTCAGCTGA TAAAAACCCT CCAGCGCTAC AGAGACATCG ACACCGCGGA AACCGGACTC 
    ATCACACAAG AACAATATGA ACAGGTTGAA TTATGGTTTC CCAGTGAACA AGATCCTTCT 
    GTCCCTGATG ACCCAACTGA ACCCGTGCCC TATGACAGAT TGGCCAACCT TAAAAAGTTT 
    TTCTTCAGTC TGTTTGCGAG CACACACTCC TCACCACTGC TGCTGGACTA TCTGATGATG 
    CTGCTTCATT TCTGCTGCCA TCCTGAAGCA GCTCAGGGCT TCACCAGAGC GCTCGGCCTC 
    GTCACCGGAC ACACACTGCA CTACAAACAC ACCACCCCAC TCACACAGTC TGTGCCGTGT 
    CTGGAGGGCG CTGGAGTTGT GGATGATGCT GGTGCTGATA ATGAAGAGGA GCAGCACAGA 
    GGGGTGGAGT CTGAGGAAGT GGGCGTGTCT GTTGATGATG TGCTCAGAGT TTTGAGTCAT 
    GGAGAGGATC TCGTCTTACA TCTAAACCGC TTCCAGCTTG TCCACAGGAG CAGAGATGAA 
    CTCCGAGAGG AGCTTCTGAA GGTGTTTAAG GACTTGGGTT TCAGTGGTGA AGAGAAGATT 
    CCTTTCTCCA CACTATCTCA GCATCCCTTT CTGCAGGACC TGATAGAAGG ATCCGCCTAT 
    TATCTGCTCC ATGACATTCA CAAAATTTTC CAGACAAAAC AAACTGAAGA TTCGAGAAGT 
    TTCTCAACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301184.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299459.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa41927
    Clone ID Related Accession (Same CDS sequence) XM_009301184.2 , XM_009301184.3
    Accession Version XM_009301184.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm flagellar protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299459.1
    CDS171..5048
    Translation

    Target ORF information:

    RefSeq Version XM_009301184.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    spef2 ( XM_009301184.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299459.1 Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID ODa41927
    Clone ID Related Accession (Same CDS sequence) XM_009301184.2 , XM_009301184.3
    Accession Version XM_009301184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299459.1
    CDS202..5079
    Translation

    Target ORF information:

    RefSeq Version XM_009301184.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm flagellar 2 (spef2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009301184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTAAAG CGGTGATGCT GGGCAGGCAT GGAGCGGCCA CTCGCCTGCT GTACCAACTC 
    TACATACTCC TGCAGAGAAA GAAGAGAGCA GGACTCACTG CCACAGCCAT GGAGGTCATG 
    CAGCCTGCAG CCACGGCCCG CCTACACCGC ATAGAGAACA GCATCTACAC TGAGCGCTTG 
    AAAACAGTGG TGAAGCGTGA GGCGGATCTG AAGATGCAGA AGATCGCAAA GCGCTTTCAT 
    AAAAGAGGGC AAGAAATGTT CAGCCGCTCA GTGATGACTG AATTGCTGAA GGAGGAGGAC 
    CGGCTCAAAC GCCAGGAGGA GAGAAGACTG AGGGACATAG AAAAGCATCG GCAGGCACGC 
    AGAAAACAAA ATGAGATTAT GATTCAGATC CAGTCGGCTG TTGTTCAGAT CCCAAAACCT 
    CCACACATTC GCACGTCAAG AGCTTCAGAG AAACAACAGC AGTTTCGCAA AAAGCAAGAA 
    GCTCAGCATG TTCATCGTGA GATAGCACAG TTTGAGAAAA AAAAGAAGAC CACTTCTCCT 
    CCTGAAGGTG GAGCTTTACT CTTCAGTGGT CGTGTGACAC AGCCGATGAG TACAGGAGAC 
    AAAGCTCAGT GGAACAAGGA CTACATCGAG AACATCCGTC AGCGTCTGGA GGAAGACGCC 
    ACTGCACGGG AACAGAGAGA GATGAGGAGA CGCCGAGCGC TGATGGAGCA ACTGCAGGCT 
    CACGAGGCTC AGCAGGAGGC ATTGCGAGAA GAACAAATGG TCAGCCGACT GATGCGTCAG 
    ACACAGCAGG AGAAAAGGAT CACAGTTCAG CTGATGCAGA TTAAACAGCA GAAAGAGGTG 
    CTTCGAGAAA ACCGCATCTT TCAGGAGAGA CAATACCAGG AACGCCGCCA ACGAGACTTT 
    CAGGAGGCCT TGGATAGAGA AGCTGTTCTC GCTCAGCAGG AGCGTCTGGA GCGCAGTGAA 
    CAGCTCAGGA TAGAGCATGA GCTGCACAAA CAGCTGGCTG AAGAGCGCGC TCAAGCTCGA 
    TATCAGAAGC ACTTTGGTAT CTGCAGGGAC ATTCTGGGAC AGATTGTTGA TCTGGCAACC 
    AAAGCTGGAG AATATCGACT TCTCACTGCC AATCTGATAC CAGTGAAGCT GATGCAGGAG 
    TGGATGGAGC TGTTTTACAG TGGGAAGCCG CTGTATGAAG TGGCCTGTGT GGATCCTAAA 
    CCTACTGAAC CCACACCAGA GCAGATCATT GAGCTTGAGA AGCTCCAGAT ACTCAACAAT 
    CAAGACTACG ATGAGTATGT GGCCATGATA GGCGAGTGGG CTTTAGATGA AGAAACCGAG 
    AGACAAACCA ATGCAGGGAA TAGTGATATT CTGGATCATG TGTTGAACCG TCTACGGAAC 
    ATGATTGTCC CACCCACCGT CAGCACTCCT CCTCCTCTGT TTCCCCGTTT CACCATCAGG 
    GCATGCATGC TGGGAAAAAC CTTCTCTGGC AAAACCACCT GCCTCTCCAG GATCAGCAGC 
    ACTCTTAACA TCCATGTACT CTCAGCCAAC ACTTTGATCC AGGACGCTTT ATCAGCCTAT 
    CAGCAGGAAA AACAGGCTGC AAGCGAAAGC CAGAATGAGA AGAGCTTGGA GAAAAATGTT 
    TCTCAGACAG AACATACTAC AGAACAGCTT CAGAAAGATG AGCAGGAGAC ATCCTCTCCT 
    CTAGAATCAG GACCACAGCC TCATGAGGCC TCATCTGGGC TTCATGAAAA AGAGGACAAA 
    GAAGAATCAA AGGCAATCCC AAAGTGGTCA TCACGAGCAC AGCACGGTGC AGCGGTAGAG 
    AAAGTTCTGA GGACAGGCCA AGCTGTTCCT GATCAGCTTC TGGTGGACAT TATTGTGGAT 
    GCTATCAGGA ACATTCCTGC AGATAGTGGC TGGATTCTTG ATGGCTTCCC GGTGGACATC 
    AGTCAGGCTC AGATGCTTGA GAAAGCTCTA AACATGACTG AACTTGATGG AACAAAAACA 
    CAAAATAATT TGTCCACAAA CAAAAACGGC CCTAAAGATG CGGCTCCTCC ATCCCCTGCT 
    CTAGACCTAG TGGTGCTGTT AGACGTTTCA GATGAGCAGG TTCTGGAGCG AGCCACAACT 
    CAGCCTCCTG AGGGAACACA GATAGTGAAT GGAGAAAGCA TAAAGCAGGA GAAAGGCAGC 
    AGTGATGCTG CGGAGGAAAA CACAAGCATG CCAGATCTGA ACACACAAGA TCCTGAATCC 
    TTGTCTGACG ACAAAAGCCT GGGGAGGACA CAAATACAGT TCAGAATCAG TGGCTTTCAT 
    CAAACATGGC CAAAACTGGA GAAGTGGTTT GGTGATCAGC AGAATATTCT GGTGAAAGTC 
    ACAGCAGGTG TGGACAAGGA CACTCTCTTC AGTAACGTGG AGACTATTCT GAGGGACACT 
    ATTGAGTCTG TTGAAAAAGG AGCAGCTCTG GCTGACTCCA GGAGAGCTTC AGTGACCTCC 
    ACACCTGAAC AGACCCGTCC AGAAAGTGCC TGCTCTTCCA AATCTTCCCG AGCTACCCCA 
    AAATCTGCTA GAAGAAGCTT TGCGGAGGAA CCTTTACCCA AGGAGTTGGC AGAATATCTT 
    GTGCCATACT GGGAGAACTT CTGTAGTTCA TATGTGACTA ATGTCAAAAC AGTGATGCAG 
    AATCTCCGTG GTGAACGTGA GCTCATCATT CATCACCTTT ACAACATCCG GGAGAACTTC 
    AGGCAGTATC TACAGAAGCC GGATCTGAAG CAGGAGTTTG TTAGCTCATG GCAGCGAGAT 
    TATAACAGTG TTCCTGATAA CATCAGAGAG GACGAGGAGA CCAAAGGAGA GCTTCATCAA 
    AGGCTGGATG ACTTGCGTGA GCGGCTATGG GACATCAGTG ATAAGCGTAA AGACGAGGCA 
    GAGCAGGAGA AAGCAGGCGT CATTCATGAT GGCTGGTTAG AGGATCACAC CGCTGTACTA 
    ATCAACAATT ATTCTGCATT GATGCAGATA GAAGTGGGCC GATTTCAGGA CTGTGTGTGT 
    CTGCTGAAAG ATTATTATTC AACGATGGGT AAAACAGCCT TTGAATCCAA ATGTGACTTT 
    GCTCGTGTCC CATTGCTTGA CATCACCGCT GATCAGCATG TCGACGAGTC GAAAATCTCT 
    GAATCTGCTC CGTCAGAGAA ATCTGTAAAG AATGCTGAGG AAAAAGACTC TGAAATTGTG 
    GACAGGATGA AAACTAAAGT GTTTCCACTG GTTTCCTGCA GATCTCCAAC TTCTGAGATC 
    CTCAAACAGC CTTTGCATCA GGCTGATGAG AACCTGCTGC AGGAGTTCTT CCAAGCAGCA 
    ATCGCCATCT TCAGGAGCAT GGTGTCGGCA GAGACGCGGC AGTGGGATGA AGAAGAAGTT 
    GATGAAGACC AGAAGCAGAT GGAGGCACAG AAAGCTCAAA CTTTGACCTC TGCTACAGAC 
    GTAAGGAAAG CAGGAAAACA AAAAGGTGCT CCACCTCCTC CTCCTCCAGA GCCCAGTCCT 
    CAACCTGCTG CAAGGAACCC AGAGGAGCTC AGGAAGAGAG CGGAGAAGAA ACGATTCAAA 
    CAGGAGCTCT CAGCTGCACT TAAACCTGAA GAAAATGCAG TGAAGCAGCG TTTGGAGCTA 
    GTGAAGGCTC ATGGGCTGAG AACAGTCATC AGTCTGCAGC ACAAAGCAGA ACAGGTCTAT 
    AGGAACATGG AGGAGTGGAT GAAAGCCCGC TATCTGGCTG AAATGAAAAG TATCGACCAG 
    CTGACAGATT TGGTGAGGCA GCACATTGAG ACTGGAGTTC AGATCCGTCA TGAGCTGGTT 
    CTGCAGAGCA CTGATTTCTG TGTGGATGGA GACACCCGTG TGCTGTTGAG TCCAGAGCTT 
    ACTCCGCGCC GATCCCTGCT GGAGCAGCCT AAAAACAGCA CACTCACAGT ACAACAGCTG 
    CACGTCCTCT GCACACAGTT GCGCAAGATC GCCCCTAAAG GTCTGATGTG TAGAAGTGAG 
    CTGACTGAAG CTCTACACGA ATTAACCTCT GCTCACATGG GTACTGATGT CCTTCCTGAG 
    GCCTGGATGC ACATCACTCC TTCACAGGTT GAGGAGCTGG TGTGTATTCT AGCTCAGGAC 
    TCTGAGATGT TGGACTGGCG TCAGTTTCTG CTCAGTGCTG CTCTGCCGTG GCCATTTCCC 
    ACTCAGACTC AGCTGATAAA AACCCTCCAG CGCTACAGAG ACATCGACAC CGCGGAAACC 
    GGACTCATCA CACAAGAACA ATATGAACAG GTTGAATTAT GGTTTCCCAG TGAACAAGAT 
    CCTTCTGTCC CTGATGACCC AACTGAACCC GTGCCCTATG ACAGATTGGC CAACCTTAAA 
    AAGTTTTTCT TCAGTCTGTT TGCGAGCACA CACTCCTCAC CACTGCTGCT GGACTATCTG 
    ATGATGCTGC TTCATTTCTG CTGCCATCCT GAAGCAGCTC AGGGCTTCAC CAGAGCGCTC 
    GGCCTCGTCA CCGGACACAC ACTGCACTAC AAACACACCA CCCCACTCAC ACAGTCTGTG 
    CCGTGTCTGG AGGGCGCTGG AGTTGTGGAT GATGCTGGTG CTGATAATGA AGAGGAGCAG 
    CACAGAGGGG TGGAGTCTGA GGAAGTGGGC GTGTCTGTTG ATGATGTGCT CAGAGTTTTG 
    AGTCATGGAG AGGATCTCGT CTTACATCTA AACCGCTTCC AGCTTGTCCA CAGGAGCAGA 
    GATGAACTCC GAGAGGAGCT TCTGAAGGTG TTTAAGGACT TGGGTTTCAG TGGTGAAGAG 
    AAGATTCCTT TCTCCACACT ATCTCAGCAT CCCTTTCTGC AGGACCTGAT AGAAGGATCC 
    GCCTATTATC TGCTCCATGA CATTCACAAA ATTTTCCAGA CAAAACAAAC TGAAGATTCG 
    AGAAGTTTCT CAACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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