Spef2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spef2
Entrez Gene ID	64555
Full Name	sperm flagellar 2
Synonyms	Kpl2
General protein information	Preferred Names sperm flagellar 2 Names sperm flagellar protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q16
Summary	may play a role in differentiation or function of ciliated cells in the airway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591091.1	XP_017446580.1	sperm flagellar protein 2 isoform X1
XM_017591092.1	XP_017446581.1	sperm flagellar protein 2 isoform X2
XM_017591094.1	XP_017446583.1	sperm flagellar protein 2 isoform X3
NM_022620.1	NP_072142.1	sperm flagellar protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPEF2	XP_546353.2
Gallus gallus (chicken)	SPEF2	XP_003643024.2
Danio rerio (zebrafish)	spef2	NP_001121704.1
Homo sapiens (human)	SPEF2	NP_079143.3
	LOC703498	XP_001091802.1
Mus musculus (house mouse)	Spef2	NP_796097.2
Pan troglodytes (chimpanzee)	SPEF2	XP_001143539.2
Bos taurus (cattle)	SPEF2	XP_002696427.2
Rattus norvegicus (Norway rat)	Spef2	NP_072142.1
Xenopus tropicalis (tropical clawed frog)	spef2	XP_002940003.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Sironen A, Kotaja N, Mulhern H, Wyatt TA, Sisson JH, Pavlik JA, Miiluniemi M, Fleming MD, Lee L
Biology of reproduction85(4)690-701(2011 Oct)

Expression of SPEF2 during mouse spermatogenesis and identification of IFT20 as an interacting protein.
Sironen A, Hansen J, Thomsen B, Andersson M, Vilkki J, Toppari J, Kotaja N
Biology of reproduction82(3)580-90(2010 Mar)

Cloning and characterization of KPL2, a novel gene induced during ciliogenesis of tracheal epithelial cells.
Ostrowski LE, Andrews K, Potdar P, Matsuura H, Jetten A, Nettesheim P
American journal of respiratory cell and molecular biology20(4)675-83(1999 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa59870	XM_017591092.1 Latest version!	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
ORa59869	XM_017591091.1 Latest version!	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa59871	XM_017591094.1 Latest version!	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa06153	NM_022620.1 Latest version!	Rattus norvegicus sperm flagellar 2 (Spef2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spef2 ( XM_017591092.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446581.1 Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59870
Clone ID Related Accession (Same CDS sequence)	XM_017591092.1
Accession Version	XM_017591092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGAGACTT 
AAAAACCTGA TACCTCGCCA AACCGATTTC AATCTGATGC GGGTTACATG CAGGTTTCAA 
GAAAAGTGTA AACAAATGAA AGAAGATCTT GCCCGAATGC ATTTTGAGAA GTTTGAAAAA 
ATTCAAAAAC TTGAGGAAGA GCAAAGACAC TTTAATATCG AAAAGCAACG CTTAAACAGA 
AGACGACAAA ATGAGATAAT GGCCAAAATC CAGGCAGCCA TTATACAGAT TCCCAAGCCT 
GAACCCAACC GTACCTTGAA AGCTATTGAG GCTCAAAAAC TGATGAAAAA GAAAAAGGAG 
GCAGAGGACG TGGCCAATGA GATTAAGAAA TTTGAAGCAC TAATTAAAAA GGATCTCCAA 
ACTAGAGAAA GTATATCCAA GACTTCTTTA GATACAATAG ACCAGACAGC TGCGGAACTG 
CTCAACACTT ACTCAGATGA CGACTACATT AAAAAGATCC AGAAACGCCT AGAAGAAGAT 
GCTTTTGCAC GTGAACAAAG GGAAAAAAGA CGACGGAGGT TGTTGATGGA CCAGCTGATA 
GCCCATGAAG CACAGGAGGA GGCCTATCGG GAAGAGCAGC TCATTCACCG GCTCATGCGC 
CAGTCCCAGC AGGAGCGCAG GATTGCTGTG CAGCTCATGC ACGTTCGCCA CGAGAAGGAA 
GTTTTGTGGC AAAATAGAAT TTTCAGAGAG AAACAATTTG AAGAAAGACG ACTTAAGGAT 
TTCCAGGATG CTCTAGATAG AGAAGCGGCT CTCACCAAAC AAGCCAAGAT TGATTTTGCA 
GAGCAAACCT TGAGGGCGAA GGAAATTCAT GAAAAGATCG CCATGGAAAG AGCCCAACAG 
CGTTACGAGA AGCATTATGG GATATGTGCA GAGATTTTGG ATCAAATACT TGATTTGTGC 
ACGAAGGTAG CAGACTATAG GTTGCTGACA AATAATCTTA TTCCACACAA GATGATGCGT 
GATTGGAAAG AACTGTTTTT TAGTGGAAAT CCCATATATG AACAGACTTC TCATACACAT 
GGACAAACAG AATCCACTGA GGATCACCAT GTAGAGTTGA ACAAAAGGAA CTTACTCGAT 
AGCAAAGATT ATGAAGACTA TAAGGTACAA ACTAATATGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446581.1
CDS	79..1623
Translation	MSEILCQWLNQELKVSRTVSPKSFAKTFSNGYLIGEVLFKFELQSDFSEFSESRVSTAKLNNFSRLQPTLHLLGVQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNQRLQALKSEAFRERLKNLIPRQTDFNLMRVTCRFQEKCKQMKEDLARMHFEKFEKIQKLEEEQRHFNIEKQRLNRRRQNEIMAKIQAAIIQIPKPEPNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQTRESISKTSLDTIDQTAAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALTKQAKIDFAEQTLRAKEIHEKIAMERAQQRYEKHYGICAEILDQILDLCTKVADYRLLTNNLIPHKMMRDWKELFFSGNPIYEQTSHTHGQTESTEDHHVELNKRNLLDSKDYEDYKVQTNME

Target ORF information:

RefSeq Version	XM_017591092.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591092.1

ORF Insert Sequence:

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGAGACTT 
AAAAACCTGA TACCTCGCCA AACCGATTTC AATCTGATGC GGGTTACATG CAGGTTTCAA 
GAAAAGTGTA AACAAATGAA AGAAGATCTT GCCCGAATGC ATTTTGAGAA GTTTGAAAAA 
ATTCAAAAAC TTGAGGAAGA GCAAAGACAC TTTAATATCG AAAAGCAACG CTTAAACAGA 
AGACGACAAA ATGAGATAAT GGCCAAAATC CAGGCAGCCA TTATACAGAT TCCCAAGCCT 
GAACCCAACC GTACCTTGAA AGCTATTGAG GCTCAAAAAC TGATGAAAAA GAAAAAGGAG 
GCAGAGGACG TGGCCAATGA GATTAAGAAA TTTGAAGCAC TAATTAAAAA GGATCTCCAA 
ACTAGAGAAA GTATATCCAA GACTTCTTTA GATACAATAG ACCAGACAGC TGCGGAACTG 
CTCAACACTT ACTCAGATGA CGACTACATT AAAAAGATCC AGAAACGCCT AGAAGAAGAT 
GCTTTTGCAC GTGAACAAAG GGAAAAAAGA CGACGGAGGT TGTTGATGGA CCAGCTGATA 
GCCCATGAAG CACAGGAGGA GGCCTATCGG GAAGAGCAGC TCATTCACCG GCTCATGCGC 
CAGTCCCAGC AGGAGCGCAG GATTGCTGTG CAGCTCATGC ACGTTCGCCA CGAGAAGGAA 
GTTTTGTGGC AAAATAGAAT TTTCAGAGAG AAACAATTTG AAGAAAGACG ACTTAAGGAT 
TTCCAGGATG CTCTAGATAG AGAAGCGGCT CTCACCAAAC AAGCCAAGAT TGATTTTGCA 
GAGCAAACCT TGAGGGCGAA GGAAATTCAT GAAAAGATCG CCATGGAAAG AGCCCAACAG 
CGTTACGAGA AGCATTATGG GATATGTGCA GAGATTTTGG ATCAAATACT TGATTTGTGC 
ACGAAGGTAG CAGACTATAG GTTGCTGACA AATAATCTTA TTCCACACAA GATGATGCGT 
GATTGGAAAG AACTGTTTTT TAGTGGAAAT CCCATATATG AACAGACTTC TCATACACAT 
GGACAAACAG AATCCACTGA GGATCACCAT GTAGAGTTGA ACAAAAGGAA CTTACTCGAT 
AGCAAAGATT ATGAAGACTA TAAGGTACAA ACTAATATGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( XM_017591091.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446580.1 Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa59869
Clone ID Related Accession (Same CDS sequence)	XM_017591091.1
Accession Version	XM_017591091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATT 
CCCAAGCCTG AACCCAACCG TACCTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA CTAGAGAAAG TATATCCAAG ACTTCTTTAG ATACAATAGA CCAGACAGCT 
GCGGAACTGC TCAACACTTA CTCAGATGAC GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGATG CTTTTGCACG TGAACAAAGG GAAAAAAGAC GACGGAGGTT GTTGATGGAC 
CAGCTGATAG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA CGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCAGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTAGATAGA GAAGCGGCTC TCACCAAACA AGCCAAGATT 
GATTTTGCAG AGCAAACCTT GAGGGCGAAG GAAATTCATG AAAAGATCGC CATGGAAAGA 
GCCCAACAGC GTTACGAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATACTT 
GATTTGTGCA CGAAGGTAGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACACAAG 
ATGATGCGTG ATTGGAAAGA ACTGTTTTTT AGTGGAAATC CCATATATGA ACAGACTTCT 
CATACACATG GACAAACAGA ATCCACTGAG GATCACCATG TAGAGTTGAA CAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGACTAT AAGAACATGA CTGGAGAGTG GGCCTTACCA 
GAGGACATGG CAAACAGTTC ACCACCCTCC AACAATAACA TACTGGGTCA TGTGCTTCTG 
AGATTAATTG AAAACTCGTA TCCGTCTGAG AAAGAAGTTG CAGACACTGA ATTACCTTCA 
TTTGCTGTGA AGGGTTGTTT ACTGGGGAAG ACACTCAGTG GAAAAACTAC AATCCTCAAG 
GCTCTTCAAA ATGACATCCC TATTCAAGTA CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATCCAGGCAT TTAATGATGG AGAAAAAAGT AGTAAGGCAC CATCGATGCA TGAAGATGTT 
AAAGGAGATC CTGAAGTGGA CCAGAAAGAA ATGATCATAC CTCAGGATAA AAATGAGCTA 
ACAGATACTC AAGTTCCAGG GGAAGCAACA CCCCAAAAAG AAGGTACGAA GAGTAGTGAC 
TTTGAACAAT TCAGACCAAG TAATAGCTTT CTGTCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAATCAG AACTGATGCT CAGAAGAGGC AAGAGCGTTC CAGATGTACT GCTGGTTAAC 
ATCCTGGTAA ATGCTATTAA GAAGATACCT GTGAACCAAA GCTGGGTTCT AGATGGATTT 
CCAATGACAG TAAATCAAGC CAAGCTCCTG GAGGAAGCTC TTACAGGATA CAAAAGAAAA 
TCCCTGCAGT TAAAGAAGAA AAAAGCACAA ATGCCCACAT TGGCCTTGAG CCCCACAACT 
TCCAAAGAGG TCCCTCTTCT CCCATCTGCG TTCGATTTTG CCATATTGCT TGATATTTCA 
GATAACTCAT CACTGGCTCG CATTAACGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTAGCGG TCCTACAATT ACTTCAAGTG AAGAAAAGAG TGAGGACCGG 
AACTTAAGAG ACCACATACA GCACAGAATT GTTGGCTTCT TGGACAACTG GCCATTGTTG 
GAAGAGTGGT TTACAGAGCC AAGAAACATT TTGAAAAAAG TCAATGCTGA AATAGATGAA 
GCGCTCTTAT GCCAAAAAAT AAAAGAAATT TTTGCTACTG AAGTATCAAA TAAAAAAATC 
AAAGTTGAAA AGAAACTAGA AGAAAAGGAA ACTGAGAAAA AGTCAGCAGT TTCTCCGACT 
GAGCCTCCTA CGCCGACCCC TCCCCTTGCT TCTGAAGCAG AAAAAGAAAA GGAGGTTCAT 
CAGGCGAAAC CTTCTGGGAA AGGAAAGACA CAATCAGGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446580.1
CDS	79..2703
Translation	MSEILCQWLNQELKVSRTVSPKSFAKTFSNGYLIGEVLFKFELQSDFSEFSESRVSTAKLNNFSRLQPTLHLLGVQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNQRLQALKSEAFREQRLNRRRQNEIMAKIQAAIIQIPKPEPNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQTRESISKTSLDTIDQTAAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALTKQAKIDFAEQTLRAKEIHEKIAMERAQQRYEKHYGICAEILDQILDLCTKVADYRLLTNNLIPHKMMRDWKELFFSGNPIYEQTSHTHGQTESTEDHHVELNKRNLLDSKDYEDYKNMTGEWALPEDMANSSPPSNNNILGHVLLRLIENSYPSEKEVADTELPSFAVKGCLLGKTLSGKTTILKALQNDIPIQVLSIDTLVQEAIQAFNDGEKSSKAPSMHEDVKGDPEVDQKEMIIPQDKNELTDTQVPGEATPQKEGTKSSDFEQFRPSNSFLSLSMRAQLGAKSELMLRRGKSVPDVLLVNILVNAIKKIPVNQSWVLDGFPMTVNQAKLLEEALTGYKRKSLQLKKKKAQMPTLALSPTTSKEVPLLPSAFDFAILLDISDNSSLARINDIIAKEISHEISHENVSGPTITSSEEKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTEPRNILKKVNAEIDEALLCQKIKEIFATEVSNKKIKVEKKLEEKETEKKSAVSPTEPPTPTPPLASEAEKEKEVHQAKPSGKGKTQSGD

Target ORF information:

RefSeq Version	XM_017591091.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591091.1

ORF Insert Sequence:

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATT 
CCCAAGCCTG AACCCAACCG TACCTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA CTAGAGAAAG TATATCCAAG ACTTCTTTAG ATACAATAGA CCAGACAGCT 
GCGGAACTGC TCAACACTTA CTCAGATGAC GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGATG CTTTTGCACG TGAACAAAGG GAAAAAAGAC GACGGAGGTT GTTGATGGAC 
CAGCTGATAG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA CGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCAGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTAGATAGA GAAGCGGCTC TCACCAAACA AGCCAAGATT 
GATTTTGCAG AGCAAACCTT GAGGGCGAAG GAAATTCATG AAAAGATCGC CATGGAAAGA 
GCCCAACAGC GTTACGAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATACTT 
GATTTGTGCA CGAAGGTAGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACACAAG 
ATGATGCGTG ATTGGAAAGA ACTGTTTTTT AGTGGAAATC CCATATATGA ACAGACTTCT 
CATACACATG GACAAACAGA ATCCACTGAG GATCACCATG TAGAGTTGAA CAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGACTAT AAGAACATGA CTGGAGAGTG GGCCTTACCA 
GAGGACATGG CAAACAGTTC ACCACCCTCC AACAATAACA TACTGGGTCA TGTGCTTCTG 
AGATTAATTG AAAACTCGTA TCCGTCTGAG AAAGAAGTTG CAGACACTGA ATTACCTTCA 
TTTGCTGTGA AGGGTTGTTT ACTGGGGAAG ACACTCAGTG GAAAAACTAC AATCCTCAAG 
GCTCTTCAAA ATGACATCCC TATTCAAGTA CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATCCAGGCAT TTAATGATGG AGAAAAAAGT AGTAAGGCAC CATCGATGCA TGAAGATGTT 
AAAGGAGATC CTGAAGTGGA CCAGAAAGAA ATGATCATAC CTCAGGATAA AAATGAGCTA 
ACAGATACTC AAGTTCCAGG GGAAGCAACA CCCCAAAAAG AAGGTACGAA GAGTAGTGAC 
TTTGAACAAT TCAGACCAAG TAATAGCTTT CTGTCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAATCAG AACTGATGCT CAGAAGAGGC AAGAGCGTTC CAGATGTACT GCTGGTTAAC 
ATCCTGGTAA ATGCTATTAA GAAGATACCT GTGAACCAAA GCTGGGTTCT AGATGGATTT 
CCAATGACAG TAAATCAAGC CAAGCTCCTG GAGGAAGCTC TTACAGGATA CAAAAGAAAA 
TCCCTGCAGT TAAAGAAGAA AAAAGCACAA ATGCCCACAT TGGCCTTGAG CCCCACAACT 
TCCAAAGAGG TCCCTCTTCT CCCATCTGCG TTCGATTTTG CCATATTGCT TGATATTTCA 
GATAACTCAT CACTGGCTCG CATTAACGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTAGCGG TCCTACAATT ACTTCAAGTG AAGAAAAGAG TGAGGACCGG 
AACTTAAGAG ACCACATACA GCACAGAATT GTTGGCTTCT TGGACAACTG GCCATTGTTG 
GAAGAGTGGT TTACAGAGCC AAGAAACATT TTGAAAAAAG TCAATGCTGA AATAGATGAA 
GCGCTCTTAT GCCAAAAAAT AAAAGAAATT TTTGCTACTG AAGTATCAAA TAAAAAAATC 
AAAGTTGAAA AGAAACTAGA AGAAAAGGAA ACTGAGAAAA AGTCAGCAGT TTCTCCGACT 
GAGCCTCCTA CGCCGACCCC TCCCCTTGCT TCTGAAGCAG AAAAAGAAAA GGAGGTTCAT 
CAGGCGAAAC CTTCTGGGAA AGGAAAGACA CAATCAGGTG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( XM_017591094.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446583.1 Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa59871
Clone ID Related Accession (Same CDS sequence)	XM_017591094.1
Accession Version	XM_017591094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC TTAGCAACTG GTTGCCCTGG GCGACGGGAA GACGCAGTCC AGCTGACTGT 
TCCTGGAAAG TTCCTAGCAC CTGCAAGCAG TCAAATGCGC CAGTGAGCTA TGTCAGAGAT 
CCTGTGCCAG TGGCTCAACC AGGAGTTGAA GGTGTCTCGG ACCGTCCCAA GTCATTTGCA 
AAGACATTTT CTAATGGCTA TCTAATTGGA GAAGTTCTGT TCAAGTTTGA ACTTCAGAGT 
GATTTTTCAG AGTTTTCAGA GAGCAGGGTC TCAACTGCCA AACTTAATAA TTTCTCCCGC 
TTGCAACCAA CGCTTCACCT GTTGGGTGTG CAGTTTGATC AGAATGTGGC TCAGAGCATC 
ATCACAGAAA AGCCCGGGGC AGCAACCAAA CTTCTGTATC AGTTGTACAT TGCTCTTCAG 
AAAAAGAAGA AAACTGGGCT GACTGGATTA GAGATACAAA CCATGCAACC CCAAACAAAC 
CAGCGGCTTC AGGCCCTCAA GAGTGAGGCT TTTCGAGAGA GACTTAAAAA CCTGATACCT 
CGCCAAACCG ATTTCAATCT GATGCGGGTT ACATGCAGGT TTCAAGAAAA GTGTAAACAA 
ATGAAAGAAG ATCTTGCCCG AATGCATTTT GAGAAGTTTG AAAAAATTCA AAAACTTGAG 
GAAGAGCAAA GACACTTTAA TATCGAAAAG CAACGCTTAA ACAGAAGACG ACAAAATGAG 
ATAATGGCCA AAATCCAGGC AGCCATTATA CAGATTCCCA AGCCTGAACC CAACCGTACC 
TTGAAAGCTA TTGAGGCTCA AAAACTGATG AAAAAGAAAA AGGAGGCAGA GGACGTGGCC 
AATGAGATTA AGAAATTTGA AGCACTAATT AAAAAGGATC TCCAAACTAG AGAAAGTATA 
TCCAAGACTT CTTTAGATAC AATAGACCAG ACAGCTGCGG AACTGCTCAA CACTTACTCA 
GATGACGACT ACATTAAAAA GATCCAGAAA CGCCTAGAAG AAGATGCTTT TGCACGTGAA 
CAAAGGGAAA AAAGACGACG GAGGTTGTTG ATGGACCAGC TGATAGCCCA TGAAGCACAG 
GAGGAGGCCT ATCGGGAAGA GCAGCTCATT CACCGGCTCA TGCGCCAGTC CCAGCAGGAG 
CGCAGGATTG CTGTGCAGCT CATGCACGTT CGCCACGAGA AGGAAGTTTT GTGGCAAAAT 
AGAATTTTCA GAGAGAAACA ATTTGAAGAA AGACGACTTA AGGATTTCCA GGATGCTCTA 
GATAGAGAAG CGGCTCTCAC CAAACAAGCC AAGATTGATT TTGCAGAGCA AACCTTGAGG 
GCGAAGGAAA TTCATGAAAA GATCGCCATG GAAAGAGCCC AACAGCGTTA CGAGAAGCAT 
TATGGGATAT GTGCAGAGAT TTTGGATCAA ATACTTGATT TGTGCACGAA GGTAGCAGAC 
TATAGGTTGC TGACAAATAA TCTTATTCCA CACAAGATGA TGCGTGATTG GAAAGAACTG 
TTTTTTAGTG GAAATCCCAT ATATGAACAG ACTTCTCATA CACATGGACA AACAGAATCC 
ACTGAGGATC ACCATGTAGA GTTGAACAAA AGGAACTTAC TCGATAGCAA AGATTATGAA 
GACTATAAGA ACATGACTGG AGAGTGGGCC TTACCAGAGG ACATGGCAAA CAGTTCACCA 
CCCTCCAACA ATAACATACT GGGTCATGTG CTTCTGAGAT TAATTGAAAA CTCGTATCCG 
TCTGAGAAAG AAGTTGCAGA CACTGAATTA CCTTCATTTG CTGTGAAGGG TTGTTTACTG 
GGGAAGACAC TCAGTGGAAA AACTACAATC CTCAAGGCTC TTCAAAATGA CATCCCTATT 
CAAGTACTTT CTATTGATAC TCTTGTCCAA GAGGCTATCC AGGCATTTAA TGATGGAGAA 
AAAAGTAGTA AGGCACCATC GATGCATGAA GATGTTAAAG GAGATCCTGA AGTGGACCAG 
AAAGAAATGA TCATACCTCA GGATAAAAAT GAGCTAACAG ATACTCAAGT TCCAGGGGAA 
GCAACACCCC AAAAAGAAGG TACGAAGAGT AGTGACTTTG AACAATTCAG ACCAAGTAAT 
AGCTTTCTGT CACTTAGCAT GCGAGCTCAA CTTGGTGCAA AATCAGAACT GATGCTCAGA 
AGAGGCAAGA GCGTTCCAGA TGTACTGCTG GTTAACATCC TGGTAAATGC TATTAAGAAG 
ATACCTGTGA ACCAAAGCTG GGTTCTAGAT GGATTTCCAA TGACAGTAAA TCAAGCCAAG 
CTCCTGGAGG AAGCTCTTAC AGGATACAAA AGAAAATCCC TGCAGTTAAA GAAGAAAAAA 
GCACAAATGC CCACATTGGC CTTGAGCCCC ACAACTTCCA AAGAGGTCCC TCTTCTCCCA 
TCTGCGTTCG ATTTTGCCAT ATTGCTTGAT ATTTCAGATA ACTCATCACT GGCTCGCATT 
AACGATATCA TAGCTAAAGA AATCTCACAT GAAATTTCTC ATGAAAATGT TAGCGGTCCT 
ACAATTACTT CAAGTGAAGA AAAGAGTGAG GACCGGAACT TAAGAGACCA CATACAGCAC 
AGAATTGTTG GCTTCTTGGA CAACTGGCCA TTGTTGGAAG AGTGGTTTAC AGAGCCAAGA 
AACATTTTGA AAAAAGTCAA TGCTGAAATA GATGAAGCGC TCTTATGCCA AAAAATAAAA 
GAAATTTTTG CTACTGAAGT ATCAAATAAA AAAATCAAAG TTGAAAAGAA ACTAGAAGAA 
AAGGAAACTG AGAAAAAGTC AGCAGTTTCT CCGACTGAGC CTCCTACGCC GACCCCTCCC 
CTTGCTTCTG AAGCAGAAAA AGAAAAGGAG GTTCATCAGG CGAAACCTTC TGGGAAAGGA 
AAGACACAAT CAGAAACCCA GCATGGTAAG CAAGAATCTC AACCTGAAGG AAAAGGGAAG 
AAAGTTTCTC CTAAAGGAAA AGCACAAGGA GGCAAAATTT CCGTAAAGAA ATCACCCATT 
GACTCAACAG AAGTATCACC CACACTAATA GCAATGCCGA CAACTAAGCC AGGATCAGAA 
GAATGGGTCT ATGTGACTGA GCCTATACCT GAGGAGCTAC CTTCGTTTTT AGTACCTTAC 
TGGGAACTGA TAGAGAAAAC CTACATAAGC CACATCAAAA CAGTGCTTCG ACAGCTCAGA 
GAAAGCCAAC ACACCGTGCT CTCTTATTTA TATGAGACTA GGACAAGTTT TCAGGAGTTT 
CTAAGGCGTC CAGATCACAA GCAAGATTTT GTATCTCAGT GGCAGGCTGA TTTCAACTCA 
GTTCCTGAAG ACCTGTGGGA AGATGAGGAA ACAAAGGCTG AACTACACCA AAGAGTAAAT 
GATCTGCGAG ACCGCCTGTG GGACATCTGT GAAGCGAGGA AGGAAGAGGC AGAGCAGGAG 
AGACTTGATA TCATTAACGA GAGCTGGCTG CAGGACTATA TCGGAATAAC AATGAATCAC 
TTCTTTTCCC TGATGCAGGC AGAAGTGAAC CGTTTCCAAG ATACCAAAAG ACTCCTTCAA 
GATTATTACA GAGCAATGGA ATCCAAAATC CCACTGGAAG ACAATAAGAA ATTTACTCGA 
ATCCCATTGG TTCAACTCGA TGGTAAAGAA ATTTCAGAAC ACCAGCTCAG AATTCCTCTA 
GTTCCTCGAA TATCCAGTTC TCCAGAAAAT GTTAGCATGA GACCAAAGGT GAAAGCATTA 
CTGAAGGGCA GATTCGATCC ACCACTGGAA AGTCTGGAAG CCAACTTTGA AGGAGATGAG 
AAGATAGTAT TAGACACCTG GCAGCAGGCT TCCTTAGCCA TCTCCCAAAT GGTGGCTGCT 
GAAGTTCATC AAAGGCTCGC AGAGGAAGAA AAGGAACAAC CCCAACTAGA TCCCAAAGAA 
AAGTCTCCTC AGTCGGGTGC AAACAAAAAG GCCAAGAAGG AGAAAGAACC GCCCAAGAAG 
AAAATGGCAG AAAAGAAAGG AAAAGGTAAA TCATCCCCTG TGGTAGAAGT CAGTCCCGTC 
ACAATAACGC CCGAGGAAAT GGCTGAAATG GAAAGGAAGA ATGAGCTGAA GCTCAAGATA 
AAGGAAGAAC ATCTTGCCGC CCTGCAGACA GAAGAACAAG CCACACAATT TCGACTTGAA 
CTAATAAAGT CAAAAGCTTT GTCCATCCTG GAAGATTTAG TAACAAAGGT GATTGAGGTT 
TACAGGTTCA TGGAAAAATG GCTTGGTAAG AGATATTTGA ATGAAATGGC CAGTATACAG 
AAATTGACCG AAGTAGCTCG CTACCACATT GAGACAGCTA CGAAAATTCA AAATGAGATT 
TATTTAAGCC AAGAAGACTT CTACATCAAT GGAGACATAA AAGTCTTCCC TGACCCTCCT 
CCACCAACCC GCCCACCACC GGTAGAAAAG GAAGAAAATG GCACCCTAAC AATCGAACAG 
CTTGATAACC TTCGAGATCA GTTCTTAGAT ATGGCGCCTA AAGGCATAAT AGGAAATAAA 
GCTTTCACTG ACATTCTGCT TGATCTGGTT ACCCTGAACC TGGGAACAAA CAACTTGCCT 
AGCAGCTGGA TGCACCTCAC CCAACTGGAT TTGCAGGAGA TAACATCTTT ATTAACAGTC 
AACACAGAGT TCGTGGACTG GAGGAAGTTC TTGATGGTAG CAGCCATGCC ATGGCCCATC 
CCCCTGGAGG AGGAGCTCCT GGAGACCCTG CAGAGGTTTA AGGCCACCGA TGAAGCTCAG 
ACAGGCACCA TCACCTTTGA ACAATATAAG CAGGCTGGTC TATGGTTTTC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCCCTTGAA CCCTTGCCAT TCAATAGACA GGAGCATCTC 
ATAGAGTTCT TCTTCAGGCT GTTTGCTGAC TCTGAGAAGG ATCCACCTCA GCTTGACTAC 
ACTCAGATGT TGCTGTATTT TGCCTGCCAC CCAGACACTT TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTGGGGAC TCACATATTC CGGCAGGTTG AAACTCCAAT GCTAACTGCA 
GAAAAGACCT CCATCTCCTC TGTGAGTCCC AATGAGGAAT TCCCTGAAGC TGAAGAAAAC 
AGTACAAGAG AGGAGCTGAA GGAGGAGAAA GATGAGAGGG ACCAAAAGGA AGAAGAAATC 
CCAGAAAATG CAAACACTGA AAAGATTTCC ATGGAAACAC TGCTCAAAGT GTTTGGAGGA 
GGAAATGAAG TGCTGGACGC TAACAGATTT GCCAGCTACC TAAAGACAGA GAATATTTAC 
TCAGAGAACC TCATCAAGAC GTTCCAGGAC CTGGGTGCCA AGAACCTAGA GCCAATCGAA 
GTTAATATTC TCTTGAAGCA TCCTTACATT CAGGACCTGA TTACGAATTA TGTAGACTAT 
AAAATCCCTG ACATTAAAAT GATCCTTCAA AGGAGTGAGC ATGCACAAGG CAGTGATGGA 
GAAAGGTCAC CCTCAAGACT TACAGACGAA AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446583.1
CDS	27..5525
Translation	MSFLSNWLPWATGRRSPADCSWKVPSTCKQSNAPVSYVRDPVPVAQPGVEGVSDRPKSFAKTFSNGYLIGEVLFKFELQSDFSEFSESRVSTAKLNNFSRLQPTLHLLGVQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNQRLQALKSEAFRERLKNLIPRQTDFNLMRVTCRFQEKCKQMKEDLARMHFEKFEKIQKLEEEQRHFNIEKQRLNRRRQNEIMAKIQAAIIQIPKPEPNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQTRESISKTSLDTIDQTAAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALTKQAKIDFAEQTLRAKEIHEKIAMERAQQRYEKHYGICAEILDQILDLCTKVADYRLLTNNLIPHKMMRDWKELFFSGNPIYEQTSHTHGQTESTEDHHVELNKRNLLDSKDYEDYKNMTGEWALPEDMANSSPPSNNNILGHVLLRLIENSYPSEKEVADTELPSFAVKGCLLGKTLSGKTTILKALQNDIPIQVLSIDTLVQEAIQAFNDGEKSSKAPSMHEDVKGDPEVDQKEMIIPQDKNELTDTQVPGEATPQKEGTKSSDFEQFRPSNSFLSLSMRAQLGAKSELMLRRGKSVPDVLLVNILVNAIKKIPVNQSWVLDGFPMTVNQAKLLEEALTGYKRKSLQLKKKKAQMPTLALSPTTSKEVPLLPSAFDFAILLDISDNSSLARINDIIAKEISHEISHENVSGPTITSSEEKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTEPRNILKKVNAEIDEALLCQKIKEIFATEVSNKKIKVEKKLEEKETEKKSAVSPTEPPTPTPPLASEAEKEKEVHQAKPSGKGKTQSETQHGKQESQPEGKGKKVSPKGKAQGGKISVKKSPIDSTEVSPTLIAMPTTKPGSEEWVYVTEPIPEELPSFLVPYWELIEKTYISHIKTVLRQLRESQHTVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDYIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESKIPLEDNKKFTRIPLVQLDGKEISEHQLRIPLVPRISSSPENVSMRPKVKALLKGRFDPPLESLEANFEGDEKIVLDTWQQASLAISQMVAAEVHQRLAEEEKEQPQLDPKEKSPQSGANKKAKKEKEPPKKKMAEKKGKGKSSPVVEVSPVTITPEEMAEMERKNELKLKIKEEHLAALQTEEQATQFRLELIKSKALSILEDLVTKVIEVYRFMEKWLGKRYLNEMASIQKLTEVARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPPPPTRPPPVEKEENGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILLDLVTLNLGTNNLPSSWMHLTQLDLQEITSLLTVNTEFVDWRKFLMVAAMPWPIPLEEELLETLQRFKATDEAQTGTITFEQYKQAGLWFSGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADSEKDPPQLDYTQMLLYFACHPDTLEGVYRALSVAVGTHIFRQVETPMLTAEKTSISSVSPNEEFPEAEENSTREELKEEKDERDQKEEEIPENANTEKISMETLLKVFGGGNEVLDANRFASYLKTENIYSENLIKTFQDLGAKNLEPIEVNILLKHPYIQDLITNYVDYKIPDIKMILQRSEHAQGSDGERSPSRLTDEKK

Target ORF information:

RefSeq Version	XM_017591094.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm flagellar 2 (Spef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591094.1

ORF Insert Sequence:

ATGAGCTTCC TTAGCAACTG GTTGCCCTGG GCGACGGGAA GACGCAGTCC AGCTGACTGT 
TCCTGGAAAG TTCCTAGCAC CTGCAAGCAG TCAAATGCGC CAGTGAGCTA TGTCAGAGAT 
CCTGTGCCAG TGGCTCAACC AGGAGTTGAA GGTGTCTCGG ACCGTCCCAA GTCATTTGCA 
AAGACATTTT CTAATGGCTA TCTAATTGGA GAAGTTCTGT TCAAGTTTGA ACTTCAGAGT 
GATTTTTCAG AGTTTTCAGA GAGCAGGGTC TCAACTGCCA AACTTAATAA TTTCTCCCGC 
TTGCAACCAA CGCTTCACCT GTTGGGTGTG CAGTTTGATC AGAATGTGGC TCAGAGCATC 
ATCACAGAAA AGCCCGGGGC AGCAACCAAA CTTCTGTATC AGTTGTACAT TGCTCTTCAG 
AAAAAGAAGA AAACTGGGCT GACTGGATTA GAGATACAAA CCATGCAACC CCAAACAAAC 
CAGCGGCTTC AGGCCCTCAA GAGTGAGGCT TTTCGAGAGA GACTTAAAAA CCTGATACCT 
CGCCAAACCG ATTTCAATCT GATGCGGGTT ACATGCAGGT TTCAAGAAAA GTGTAAACAA 
ATGAAAGAAG ATCTTGCCCG AATGCATTTT GAGAAGTTTG AAAAAATTCA AAAACTTGAG 
GAAGAGCAAA GACACTTTAA TATCGAAAAG CAACGCTTAA ACAGAAGACG ACAAAATGAG 
ATAATGGCCA AAATCCAGGC AGCCATTATA CAGATTCCCA AGCCTGAACC CAACCGTACC 
TTGAAAGCTA TTGAGGCTCA AAAACTGATG AAAAAGAAAA AGGAGGCAGA GGACGTGGCC 
AATGAGATTA AGAAATTTGA AGCACTAATT AAAAAGGATC TCCAAACTAG AGAAAGTATA 
TCCAAGACTT CTTTAGATAC AATAGACCAG ACAGCTGCGG AACTGCTCAA CACTTACTCA 
GATGACGACT ACATTAAAAA GATCCAGAAA CGCCTAGAAG AAGATGCTTT TGCACGTGAA 
CAAAGGGAAA AAAGACGACG GAGGTTGTTG ATGGACCAGC TGATAGCCCA TGAAGCACAG 
GAGGAGGCCT ATCGGGAAGA GCAGCTCATT CACCGGCTCA TGCGCCAGTC CCAGCAGGAG 
CGCAGGATTG CTGTGCAGCT CATGCACGTT CGCCACGAGA AGGAAGTTTT GTGGCAAAAT 
AGAATTTTCA GAGAGAAACA ATTTGAAGAA AGACGACTTA AGGATTTCCA GGATGCTCTA 
GATAGAGAAG CGGCTCTCAC CAAACAAGCC AAGATTGATT TTGCAGAGCA AACCTTGAGG 
GCGAAGGAAA TTCATGAAAA GATCGCCATG GAAAGAGCCC AACAGCGTTA CGAGAAGCAT 
TATGGGATAT GTGCAGAGAT TTTGGATCAA ATACTTGATT TGTGCACGAA GGTAGCAGAC 
TATAGGTTGC TGACAAATAA TCTTATTCCA CACAAGATGA TGCGTGATTG GAAAGAACTG 
TTTTTTAGTG GAAATCCCAT ATATGAACAG ACTTCTCATA CACATGGACA AACAGAATCC 
ACTGAGGATC ACCATGTAGA GTTGAACAAA AGGAACTTAC TCGATAGCAA AGATTATGAA 
GACTATAAGA ACATGACTGG AGAGTGGGCC TTACCAGAGG ACATGGCAAA CAGTTCACCA 
CCCTCCAACA ATAACATACT GGGTCATGTG CTTCTGAGAT TAATTGAAAA CTCGTATCCG 
TCTGAGAAAG AAGTTGCAGA CACTGAATTA CCTTCATTTG CTGTGAAGGG TTGTTTACTG 
GGGAAGACAC TCAGTGGAAA AACTACAATC CTCAAGGCTC TTCAAAATGA CATCCCTATT 
CAAGTACTTT CTATTGATAC TCTTGTCCAA GAGGCTATCC AGGCATTTAA TGATGGAGAA 
AAAAGTAGTA AGGCACCATC GATGCATGAA GATGTTAAAG GAGATCCTGA AGTGGACCAG 
AAAGAAATGA TCATACCTCA GGATAAAAAT GAGCTAACAG ATACTCAAGT TCCAGGGGAA 
GCAACACCCC AAAAAGAAGG TACGAAGAGT AGTGACTTTG AACAATTCAG ACCAAGTAAT 
AGCTTTCTGT CACTTAGCAT GCGAGCTCAA CTTGGTGCAA AATCAGAACT GATGCTCAGA 
AGAGGCAAGA GCGTTCCAGA TGTACTGCTG GTTAACATCC TGGTAAATGC TATTAAGAAG 
ATACCTGTGA ACCAAAGCTG GGTTCTAGAT GGATTTCCAA TGACAGTAAA TCAAGCCAAG 
CTCCTGGAGG AAGCTCTTAC AGGATACAAA AGAAAATCCC TGCAGTTAAA GAAGAAAAAA 
GCACAAATGC CCACATTGGC CTTGAGCCCC ACAACTTCCA AAGAGGTCCC TCTTCTCCCA 
TCTGCGTTCG ATTTTGCCAT ATTGCTTGAT ATTTCAGATA ACTCATCACT GGCTCGCATT 
AACGATATCA TAGCTAAAGA AATCTCACAT GAAATTTCTC ATGAAAATGT TAGCGGTCCT 
ACAATTACTT CAAGTGAAGA AAAGAGTGAG GACCGGAACT TAAGAGACCA CATACAGCAC 
AGAATTGTTG GCTTCTTGGA CAACTGGCCA TTGTTGGAAG AGTGGTTTAC AGAGCCAAGA 
AACATTTTGA AAAAAGTCAA TGCTGAAATA GATGAAGCGC TCTTATGCCA AAAAATAAAA 
GAAATTTTTG CTACTGAAGT ATCAAATAAA AAAATCAAAG TTGAAAAGAA ACTAGAAGAA 
AAGGAAACTG AGAAAAAGTC AGCAGTTTCT CCGACTGAGC CTCCTACGCC GACCCCTCCC 
CTTGCTTCTG AAGCAGAAAA AGAAAAGGAG GTTCATCAGG CGAAACCTTC TGGGAAAGGA 
AAGACACAAT CAGAAACCCA GCATGGTAAG CAAGAATCTC AACCTGAAGG AAAAGGGAAG 
AAAGTTTCTC CTAAAGGAAA AGCACAAGGA GGCAAAATTT CCGTAAAGAA ATCACCCATT 
GACTCAACAG AAGTATCACC CACACTAATA GCAATGCCGA CAACTAAGCC AGGATCAGAA 
GAATGGGTCT ATGTGACTGA GCCTATACCT GAGGAGCTAC CTTCGTTTTT AGTACCTTAC 
TGGGAACTGA TAGAGAAAAC CTACATAAGC CACATCAAAA CAGTGCTTCG ACAGCTCAGA 
GAAAGCCAAC ACACCGTGCT CTCTTATTTA TATGAGACTA GGACAAGTTT TCAGGAGTTT 
CTAAGGCGTC CAGATCACAA GCAAGATTTT GTATCTCAGT GGCAGGCTGA TTTCAACTCA 
GTTCCTGAAG ACCTGTGGGA AGATGAGGAA ACAAAGGCTG AACTACACCA AAGAGTAAAT 
GATCTGCGAG ACCGCCTGTG GGACATCTGT GAAGCGAGGA AGGAAGAGGC AGAGCAGGAG 
AGACTTGATA TCATTAACGA GAGCTGGCTG CAGGACTATA TCGGAATAAC AATGAATCAC 
TTCTTTTCCC TGATGCAGGC AGAAGTGAAC CGTTTCCAAG ATACCAAAAG ACTCCTTCAA 
GATTATTACA GAGCAATGGA ATCCAAAATC CCACTGGAAG ACAATAAGAA ATTTACTCGA 
ATCCCATTGG TTCAACTCGA TGGTAAAGAA ATTTCAGAAC ACCAGCTCAG AATTCCTCTA 
GTTCCTCGAA TATCCAGTTC TCCAGAAAAT GTTAGCATGA GACCAAAGGT GAAAGCATTA 
CTGAAGGGCA GATTCGATCC ACCACTGGAA AGTCTGGAAG CCAACTTTGA AGGAGATGAG 
AAGATAGTAT TAGACACCTG GCAGCAGGCT TCCTTAGCCA TCTCCCAAAT GGTGGCTGCT 
GAAGTTCATC AAAGGCTCGC AGAGGAAGAA AAGGAACAAC CCCAACTAGA TCCCAAAGAA 
AAGTCTCCTC AGTCGGGTGC AAACAAAAAG GCCAAGAAGG AGAAAGAACC GCCCAAGAAG 
AAAATGGCAG AAAAGAAAGG AAAAGGTAAA TCATCCCCTG TGGTAGAAGT CAGTCCCGTC 
ACAATAACGC CCGAGGAAAT GGCTGAAATG GAAAGGAAGA ATGAGCTGAA GCTCAAGATA 
AAGGAAGAAC ATCTTGCCGC CCTGCAGACA GAAGAACAAG CCACACAATT TCGACTTGAA 
CTAATAAAGT CAAAAGCTTT GTCCATCCTG GAAGATTTAG TAACAAAGGT GATTGAGGTT 
TACAGGTTCA TGGAAAAATG GCTTGGTAAG AGATATTTGA ATGAAATGGC CAGTATACAG 
AAATTGACCG AAGTAGCTCG CTACCACATT GAGACAGCTA CGAAAATTCA AAATGAGATT 
TATTTAAGCC AAGAAGACTT CTACATCAAT GGAGACATAA AAGTCTTCCC TGACCCTCCT 
CCACCAACCC GCCCACCACC GGTAGAAAAG GAAGAAAATG GCACCCTAAC AATCGAACAG 
CTTGATAACC TTCGAGATCA GTTCTTAGAT ATGGCGCCTA AAGGCATAAT AGGAAATAAA 
GCTTTCACTG ACATTCTGCT TGATCTGGTT ACCCTGAACC TGGGAACAAA CAACTTGCCT 
AGCAGCTGGA TGCACCTCAC CCAACTGGAT TTGCAGGAGA TAACATCTTT ATTAACAGTC 
AACACAGAGT TCGTGGACTG GAGGAAGTTC TTGATGGTAG CAGCCATGCC ATGGCCCATC 
CCCCTGGAGG AGGAGCTCCT GGAGACCCTG CAGAGGTTTA AGGCCACCGA TGAAGCTCAG 
ACAGGCACCA TCACCTTTGA ACAATATAAG CAGGCTGGTC TATGGTTTTC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCCCTTGAA CCCTTGCCAT TCAATAGACA GGAGCATCTC 
ATAGAGTTCT TCTTCAGGCT GTTTGCTGAC TCTGAGAAGG ATCCACCTCA GCTTGACTAC 
ACTCAGATGT TGCTGTATTT TGCCTGCCAC CCAGACACTT TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTGGGGAC TCACATATTC CGGCAGGTTG AAACTCCAAT GCTAACTGCA 
GAAAAGACCT CCATCTCCTC TGTGAGTCCC AATGAGGAAT TCCCTGAAGC TGAAGAAAAC 
AGTACAAGAG AGGAGCTGAA GGAGGAGAAA GATGAGAGGG ACCAAAAGGA AGAAGAAATC 
CCAGAAAATG CAAACACTGA AAAGATTTCC ATGGAAACAC TGCTCAAAGT GTTTGGAGGA 
GGAAATGAAG TGCTGGACGC TAACAGATTT GCCAGCTACC TAAAGACAGA GAATATTTAC 
TCAGAGAACC TCATCAAGAC GTTCCAGGAC CTGGGTGCCA AGAACCTAGA GCCAATCGAA 
GTTAATATTC TCTTGAAGCA TCCTTACATT CAGGACCTGA TTACGAATTA TGTAGACTAT 
AAAATCCCTG ACATTAAAAT GATCCTTCAA AGGAGTGAGC ATGCACAAGG CAGTGATGGA 
GAAAGGTCAC CCTCAAGACT TACAGACGAA AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( NM_022620.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072142.1 Rattus norvegicus sperm flagellar 2 (Spef2), mRNA.

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CloneID	ORa06153
Clone ID Related Accession (Same CDS sequence)	NM_022620.1
Accession Version	NM_022620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-14
Organism	Rattus norvegicus(Norway rat)
Product	sperm flagellar protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF102129.1. ##Evidence-Data-START## Transcript exon combination :: AF102129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATT 
CCCAAGCCTG AACCCAACCG TACCTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA CTAGAGAAAG TATATCCAAG ACTTCTTTAG ATACAATAGA CCAGACAGCT 
GCGGAACTGC TCAACACTTA CTCAGATGAC GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGATG CTTTTGCACG TGAACAAAGG GAAAAAAGAC GACGGAGGTT GTTGATGGAC 
CAGCTGATAG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA CGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCAGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTAGATAGA GAAGCGGCTC TCACCAAACA AGCCAAGATT 
GATTTTGCAG AGCAAACCTT GAGGGCGAAG GAAATTCATG AAAAGATCGC CATGGAAAGA 
GCCCAACAGC GTTACGAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATACTT 
GATTTGTGCA CGAAGGTAGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACACAAG 
ATGATGCGTG ATTGGAAAGA ACTGTTTTTT AGTGGAAATC CCATATATGA ACAGACTTCT 
CATACACATG GACAAACAGA ATCCACTGAG GATCACCATG TAGAGTTGAA CAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGACTAT AAGAACATGA CTGGGGAGTG GGCCTTACCA 
GAGGACATGG CAAACAGTTC ACCACCCTCC AACAATAACA TACTGGGTCA TGTGCTTCTG 
AGATTAATTG AAAACTCGTA TCCGTCTGAG AAAGAAGTTG CAGACACTGA ATTACCTTCA 
TTTGCTGTGA AGGGTTGTTT ACTGGGGAAG ACACTCAGTG GAAAAACTAC AATTCTCAAG 
GCTCTTCAAA ATGACATCCC TATTCAAGTA CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATTCAGGCAT TTAATGATGG AGAAAAAAGT AGTAAGGCAC CATCGATGCA TGAAGATGTT 
AAAGGAGATC CTGAAGTGGA CCAGAAAGAA ATGATCATAC CTCAGGATAA AAATGAGCTA 
ACAGATACTC AAGTTCCAGG GGAAGCAGCA CCCCAAAAAG AAGGTACGAA GAGTAGTGAC 
TTTGAACAAT TCAGACCAAG TAATAGCTTT CTGTCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAATCAG AACTGATGCT CAGAAGAGGC AAGAGCGTTC CAGATGTACT GCTGGTTAAC 
ATCCTGGTAA ATGCTATTAA GAAGATACCT GTGAACCAAA GCTGGGTTCT AGATGGATTT 
CCAATGACAG TAAATCAAGC CAAGCTCCTG GAGGAAGCTC TTACAGGATA CAAAAGAAAA 
TCCCTGCAGT TAAAGAAGAA AAAAGCACAA ATGCCCACAT TGGCCTTGAG CCCCACAACT 
TCCAAAGAGG TCCCTTTTCT CCCATCTGCG TTCGATTTTG CCATATTGCT TGATATTTCA 
GATAACTCAT CACTGGCTCG CATTAACGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTAGCGG TCCTACAATT ACTTCAAGTG AAGAAAAGAG TGAGGACCGG 
AACTTAAGAG ACCACATACA GCACAGAATT GTTGGCTTCT TGGACAACTG GCCATTGTTG 
GAAGAGTGGT TTACAGAGCC AAGAAACATT TTGAAAAAAG TCAATGCTGA AATAGATGAA 
GCGTTCTTAT GCCAAAAAAT AAAAGAAATT TTTGCTACTG AAGTATCAAA TAAAAAAATC 
AAAGTTGAAA AGAAACTAGA AGAAAAGGAA ACTGAGAAAA AGTCAGCAGT TTCTCCGACT 
GAGCCTCCTA CGCCGACCCC TCCCCTTGCT TCTGAAGCAG AAAAAGAAAA GGAGGTTCAT 
CAGGCGAAAC CTTCTGGGAA AGGAAAGACA CAATCAGTTT CTCCTAAAGG AAAAGCACAA 
GGAGGCAAAA TTTCCGTAAA GAAATCACCC ATTGACTCAA CAGAAGTATC ACCCACACTA 
ATAGCAATGC CGACAACTAA GCCAGGATCA GAAGAATGGG TCTATGTGAC TGAGCCTATA 
CCTGAGGAGC TACCTTCGTT TTTAGTACCT TACTGGGAAC TGATAGAGAA AACCTACATA 
AGCCACATCA AAACAGTGCT TCGACAGCTC AGAGAAAGCC AACACACCGT GCTCTCTTAT 
TTATATGAGA CTAGGACAAG TTTTCAGGAG TTTCTAAGGC GTCCAGATCA CAAGCAAGAT 
TTTGTATCTC AGTGGCAGGC TGATTTCAAC TCAGTTCCTG AAGACCTGTG GGAAGATGAG 
GAAACAAAGG CTGAACTACA CCAAAGAGTT AATGATCTGC GAGACCGCCT GTGGGACATC 
TGTGAAGCGA GGAAGGAAGA GGCAGAGCAG GAGAGACTTG ATATCATTAA CGAGAGCTGG 
CTGCAGGACT ATATCGGAAT AACAATGAAT CACTTCTTTT CCCTGATGCA GGCAGAAGTG 
AACCGTTTCC AAGATACCAA AAGACTCCTT CAAGATTATT ACAGAGCAAT GGAATCCAGA 
ATCCCACTGG AAGACAATAA GAAATTTACT CGAATCCCAT TGGTTCAACT CGATGGTAAA 
GAAATTTCAG AACACCAGCT CAGAATTCCT CTAGTTCCTC GAATATCCAG TTCTCCAGAA 
AATGTTAGCA TGAGACCAAA GGTGAAAGCA TTACTGAAGG GCAGATTCGA TCCACCACTG 
GAAAGTCTGG AAGCCAACTT TGAAGGAGAT GAGAAGATAG TATTAGACAC CTGGCAGCAG 
GCTTCCTTAG CCATCTCCCA AATGGTGGCT GCTGAAGTTC ATCAAAGGCT CGCAGAGGAA 
GAAAAGGAAC AACCCCAACT AGATCCCAAA GAAAAGTCTC CTCAGTCGGG TACAAACAAA 
AAGGCCAAGA AGGAGAAAGA ACAGGCCAAG AAGGAGAAAG AACAGGCCAA AAAGGAGAAA 
GAACAGGCCA AGAAGGAGAA AGAACCGCCC AAGAAGAAAA TGGCAGAAAA GAAAGGAAAA 
GGTAAATCAT CCCCTGTGGT AGAAGTCAGT CCCGTCACAA TAACGCCCGA GGAAATGGCT 
GAAATGGAAA GGAAGAATGA GCTGAAGCTC AAGATAAAGG AAGAACATCT TGCCGCCCTG 
CAGACAGAAG AACAAGCCAC ACAATTTCGA CTTGAACTAA TAAAGTCAAA AGCTTTGTCC 
ATCCTGGAAG ATTTAGTAAC AAAGGTGATT GAGGTTTACA GGTTCATGGA AAAATGGCTT 
GGTAAGAGAT ATTTGAATGA AATGGCCAGT ATACAGAAAT TGACCGAAGT AGCTCGCTAC 
CACATTGAGA CAGCTACGAA AATTCAAAAT GAGATTTATT TAAGCCAAGA AGACTTCTAC 
ATCAATGGAG ACATAAAAGT CTTCCCTGAC CCTCCTCCAC CAACCCGCCC ACCACCGGTA 
GAAAAGGAAG AAAATGGCAC CCTAACAATC GAACAGCTTG ATAACCTTCG AGATCAGTTC 
TTAGATATGG CGCCTAAAGG CATAATAGGA AATAAAGCTT TCACTGACAT TCTGCTTGAT 
CTGGTTACCC TGAACCTGGG AACAAACAAC TTGTCTAGCA GCTGGATGCA CCTCACCCAA 
CTGGATTTGC AGGAGATAAC ATCTTTATTA ACAGTCAACA CAGAGTTCGT GGACTGGAGG 
AAGTTCTTGA TGGTAGCAGC CATGCCATGG CCCATCCCCC TGGAGGAGGA GCTCCTGGAG 
ACCCTGCAGA GGTTTAAGGC CACCGATGAA GCTCAGACAG GCACCATCAC CTTTGAACAA 
TATAAGCAGG CTGGTCTATG GTTTTCAGGA GATGAAGATA TAAAAATTCC AGAAAATCCC 
CTTGAACCCT TGCCATTCAA TAGACAGGAG CATCTCATAG AGTTCTTCTT CAGGCTGTTT 
GCTGACTCTG AGAAGGATCC ACCTCAGCTT GACTACACTC AGATGTTGCT GTATTTTGCC 
TGCCACCCAG ACACTTTGGA AGGAGTCTAC AGGGCCCTCA GTGTGGCTGT GGGGACTCAC 
ATATTCCGGC AGGTTGAAAC TCCAATGCTA ACTGCAGAAA AGACCTCCAT CTCCTCTGTG 
AGTCCCAATG AGGAATTCCC TGAAGCTGAA GAAAACAGTA CAAGAGAGGA GCTGAAGGAG 
GAGAAAGATG AGAGGGACCA AAAGGAAGAA GAAATCCCAG AAAATGCAAA CACTGAAAAG 
ATTTCCATGG AAACACTGCT CAAAGTGTTT GGAGGAGGAA ATGAAGTGCT GGACGCTAAC 
AGATTTGCCA GCTACCTAAA GACAGAGAAT ATTTACTCAG AGAACCTCAT CAAGACGTTC 
CAGGACCTGG GTGCCAAGAA CCTAGAGCCA ATCGAAGTTA ATATTCTCTT GAAGCATCCT 
TACATTCAGG ACCTGATTAC GAATTATGTA GACTATAAAA TCCCTGACAT TAAAATGATC 
CTTCAAAGGA GTGAGCATGC ACAAGGCAGT GATGGAGAAA GGTCACCCTC AAGACTTACA 
GACGAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072142.1
CDS	68..5302
Translation	MSEILCQWLNQELKVSRTVSPKSFAKTFSNGYLIGEVLFKFELQSDFSEFSESRVSTAKLNNFSRLQPTLHLLGVQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNQRLQALKSEAFREQRLNRRRQNEIMAKIQAAIIQIPKPEPNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQTRESISKTSLDTIDQTAAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALTKQAKIDFAEQTLRAKEIHEKIAMERAQQRYEKHYGICAEILDQILDLCTKVADYRLLTNNLIPHKMMRDWKELFFSGNPIYEQTSHTHGQTESTEDHHVELNKRNLLDSKDYEDYKNMTGEWALPEDMANSSPPSNNNILGHVLLRLIENSYPSEKEVADTELPSFAVKGCLLGKTLSGKTTILKALQNDIPIQVLSIDTLVQEAIQAFNDGEKSSKAPSMHEDVKGDPEVDQKEMIIPQDKNELTDTQVPGEAAPQKEGTKSSDFEQFRPSNSFLSLSMRAQLGAKSELMLRRGKSVPDVLLVNILVNAIKKIPVNQSWVLDGFPMTVNQAKLLEEALTGYKRKSLQLKKKKAQMPTLALSPTTSKEVPFLPSAFDFAILLDISDNSSLARINDIIAKEISHEISHENVSGPTITSSEEKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTEPRNILKKVNAEIDEAFLCQKIKEIFATEVSNKKIKVEKKLEEKETEKKSAVSPTEPPTPTPPLASEAEKEKEVHQAKPSGKGKTQSVSPKGKAQGGKISVKKSPIDSTEVSPTLIAMPTTKPGSEEWVYVTEPIPEELPSFLVPYWELIEKTYISHIKTVLRQLRESQHTVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDYIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESRIPLEDNKKFTRIPLVQLDGKEISEHQLRIPLVPRISSSPENVSMRPKVKALLKGRFDPPLESLEANFEGDEKIVLDTWQQASLAISQMVAAEVHQRLAEEEKEQPQLDPKEKSPQSGTNKKAKKEKEQAKKEKEQAKKEKEQAKKEKEPPKKKMAEKKGKGKSSPVVEVSPVTITPEEMAEMERKNELKLKIKEEHLAALQTEEQATQFRLELIKSKALSILEDLVTKVIEVYRFMEKWLGKRYLNEMASIQKLTEVARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPPPPTRPPPVEKEENGTLTIEQLDNLRDQFLDMAPKGIIGNKAFTDILLDLVTLNLGTNNLSSSWMHLTQLDLQEITSLLTVNTEFVDWRKFLMVAAMPWPIPLEEELLETLQRFKATDEAQTGTITFEQYKQAGLWFSGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADSEKDPPQLDYTQMLLYFACHPDTLEGVYRALSVAVGTHIFRQVETPMLTAEKTSISSVSPNEEFPEAEENSTREELKEEKDERDQKEEEIPENANTEKISMETLLKVFGGGNEVLDANRFASYLKTENIYSENLIKTFQDLGAKNLEPIEVNILLKHPYIQDLITNYVDYKIPDIKMILQRSEHAQGSDGERSPSRLTDEKK

Target ORF information:

RefSeq Version	NM_022620.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sperm flagellar 2 (Spef2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022620.1

ORF Insert Sequence:

ATGTCAGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCTCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGAC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TTCAGAGTTT TCAGAGAGCA GGGTCTCAAC TGCCAAACTT 
AATAATTTCT CCCGCTTGCA ACCAACGCTT CACCTGTTGG GTGTGCAGTT TGATCAGAAT 
GTGGCTCAGA GCATCATCAC AGAAAAGCCC GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACT GGGCTGACTG GATTAGAGAT ACAAACCATG 
CAACCCCAAA CAAACCAGCG GCTTCAGGCC CTCAAGAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATT 
CCCAAGCCTG AACCCAACCG TACCTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA CTAGAGAAAG TATATCCAAG ACTTCTTTAG ATACAATAGA CCAGACAGCT 
GCGGAACTGC TCAACACTTA CTCAGATGAC GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGATG CTTTTGCACG TGAACAAAGG GAAAAAAGAC GACGGAGGTT GTTGATGGAC 
CAGCTGATAG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA CGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCAGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTAGATAGA GAAGCGGCTC TCACCAAACA AGCCAAGATT 
GATTTTGCAG AGCAAACCTT GAGGGCGAAG GAAATTCATG AAAAGATCGC CATGGAAAGA 
GCCCAACAGC GTTACGAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATACTT 
GATTTGTGCA CGAAGGTAGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACACAAG 
ATGATGCGTG ATTGGAAAGA ACTGTTTTTT AGTGGAAATC CCATATATGA ACAGACTTCT 
CATACACATG GACAAACAGA ATCCACTGAG GATCACCATG TAGAGTTGAA CAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGACTAT AAGAACATGA CTGGGGAGTG GGCCTTACCA 
GAGGACATGG CAAACAGTTC ACCACCCTCC AACAATAACA TACTGGGTCA TGTGCTTCTG 
AGATTAATTG AAAACTCGTA TCCGTCTGAG AAAGAAGTTG CAGACACTGA ATTACCTTCA 
TTTGCTGTGA AGGGTTGTTT ACTGGGGAAG ACACTCAGTG GAAAAACTAC AATTCTCAAG 
GCTCTTCAAA ATGACATCCC TATTCAAGTA CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATTCAGGCAT TTAATGATGG AGAAAAAAGT AGTAAGGCAC CATCGATGCA TGAAGATGTT 
AAAGGAGATC CTGAAGTGGA CCAGAAAGAA ATGATCATAC CTCAGGATAA AAATGAGCTA 
ACAGATACTC AAGTTCCAGG GGAAGCAGCA CCCCAAAAAG AAGGTACGAA GAGTAGTGAC 
TTTGAACAAT TCAGACCAAG TAATAGCTTT CTGTCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAATCAG AACTGATGCT CAGAAGAGGC AAGAGCGTTC CAGATGTACT GCTGGTTAAC 
ATCCTGGTAA ATGCTATTAA GAAGATACCT GTGAACCAAA GCTGGGTTCT AGATGGATTT 
CCAATGACAG TAAATCAAGC CAAGCTCCTG GAGGAAGCTC TTACAGGATA CAAAAGAAAA 
TCCCTGCAGT TAAAGAAGAA AAAAGCACAA ATGCCCACAT TGGCCTTGAG CCCCACAACT 
TCCAAAGAGG TCCCTTTTCT CCCATCTGCG TTCGATTTTG CCATATTGCT TGATATTTCA 
GATAACTCAT CACTGGCTCG CATTAACGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTAGCGG TCCTACAATT ACTTCAAGTG AAGAAAAGAG TGAGGACCGG 
AACTTAAGAG ACCACATACA GCACAGAATT GTTGGCTTCT TGGACAACTG GCCATTGTTG 
GAAGAGTGGT TTACAGAGCC AAGAAACATT TTGAAAAAAG TCAATGCTGA AATAGATGAA 
GCGTTCTTAT GCCAAAAAAT AAAAGAAATT TTTGCTACTG AAGTATCAAA TAAAAAAATC 
AAAGTTGAAA AGAAACTAGA AGAAAAGGAA ACTGAGAAAA AGTCAGCAGT TTCTCCGACT 
GAGCCTCCTA CGCCGACCCC TCCCCTTGCT TCTGAAGCAG AAAAAGAAAA GGAGGTTCAT 
CAGGCGAAAC CTTCTGGGAA AGGAAAGACA CAATCAGTTT CTCCTAAAGG AAAAGCACAA 
GGAGGCAAAA TTTCCGTAAA GAAATCACCC ATTGACTCAA CAGAAGTATC ACCCACACTA 
ATAGCAATGC CGACAACTAA GCCAGGATCA GAAGAATGGG TCTATGTGAC TGAGCCTATA 
CCTGAGGAGC TACCTTCGTT TTTAGTACCT TACTGGGAAC TGATAGAGAA AACCTACATA 
AGCCACATCA AAACAGTGCT TCGACAGCTC AGAGAAAGCC AACACACCGT GCTCTCTTAT 
TTATATGAGA CTAGGACAAG TTTTCAGGAG TTTCTAAGGC GTCCAGATCA CAAGCAAGAT 
TTTGTATCTC AGTGGCAGGC TGATTTCAAC TCAGTTCCTG AAGACCTGTG GGAAGATGAG 
GAAACAAAGG CTGAACTACA CCAAAGAGTT AATGATCTGC GAGACCGCCT GTGGGACATC 
TGTGAAGCGA GGAAGGAAGA GGCAGAGCAG GAGAGACTTG ATATCATTAA CGAGAGCTGG 
CTGCAGGACT ATATCGGAAT AACAATGAAT CACTTCTTTT CCCTGATGCA GGCAGAAGTG 
AACCGTTTCC AAGATACCAA AAGACTCCTT CAAGATTATT ACAGAGCAAT GGAATCCAGA 
ATCCCACTGG AAGACAATAA GAAATTTACT CGAATCCCAT TGGTTCAACT CGATGGTAAA 
GAAATTTCAG AACACCAGCT CAGAATTCCT CTAGTTCCTC GAATATCCAG TTCTCCAGAA 
AATGTTAGCA TGAGACCAAA GGTGAAAGCA TTACTGAAGG GCAGATTCGA TCCACCACTG 
GAAAGTCTGG AAGCCAACTT TGAAGGAGAT GAGAAGATAG TATTAGACAC CTGGCAGCAG 
GCTTCCTTAG CCATCTCCCA AATGGTGGCT GCTGAAGTTC ATCAAAGGCT CGCAGAGGAA 
GAAAAGGAAC AACCCCAACT AGATCCCAAA GAAAAGTCTC CTCAGTCGGG TACAAACAAA 
AAGGCCAAGA AGGAGAAAGA ACAGGCCAAG AAGGAGAAAG AACAGGCCAA AAAGGAGAAA 
GAACAGGCCA AGAAGGAGAA AGAACCGCCC AAGAAGAAAA TGGCAGAAAA GAAAGGAAAA 
GGTAAATCAT CCCCTGTGGT AGAAGTCAGT CCCGTCACAA TAACGCCCGA GGAAATGGCT 
GAAATGGAAA GGAAGAATGA GCTGAAGCTC AAGATAAAGG AAGAACATCT TGCCGCCCTG 
CAGACAGAAG AACAAGCCAC ACAATTTCGA CTTGAACTAA TAAAGTCAAA AGCTTTGTCC 
ATCCTGGAAG ATTTAGTAAC AAAGGTGATT GAGGTTTACA GGTTCATGGA AAAATGGCTT 
GGTAAGAGAT ATTTGAATGA AATGGCCAGT ATACAGAAAT TGACCGAAGT AGCTCGCTAC 
CACATTGAGA CAGCTACGAA AATTCAAAAT GAGATTTATT TAAGCCAAGA AGACTTCTAC 
ATCAATGGAG ACATAAAAGT CTTCCCTGAC CCTCCTCCAC CAACCCGCCC ACCACCGGTA 
GAAAAGGAAG AAAATGGCAC CCTAACAATC GAACAGCTTG ATAACCTTCG AGATCAGTTC 
TTAGATATGG CGCCTAAAGG CATAATAGGA AATAAAGCTT TCACTGACAT TCTGCTTGAT 
CTGGTTACCC TGAACCTGGG AACAAACAAC TTGTCTAGCA GCTGGATGCA CCTCACCCAA 
CTGGATTTGC AGGAGATAAC ATCTTTATTA ACAGTCAACA CAGAGTTCGT GGACTGGAGG 
AAGTTCTTGA TGGTAGCAGC CATGCCATGG CCCATCCCCC TGGAGGAGGA GCTCCTGGAG 
ACCCTGCAGA GGTTTAAGGC CACCGATGAA GCTCAGACAG GCACCATCAC CTTTGAACAA 
TATAAGCAGG CTGGTCTATG GTTTTCAGGA GATGAAGATA TAAAAATTCC AGAAAATCCC 
CTTGAACCCT TGCCATTCAA TAGACAGGAG CATCTCATAG AGTTCTTCTT CAGGCTGTTT 
GCTGACTCTG AGAAGGATCC ACCTCAGCTT GACTACACTC AGATGTTGCT GTATTTTGCC 
TGCCACCCAG ACACTTTGGA AGGAGTCTAC AGGGCCCTCA GTGTGGCTGT GGGGACTCAC 
ATATTCCGGC AGGTTGAAAC TCCAATGCTA ACTGCAGAAA AGACCTCCAT CTCCTCTGTG 
AGTCCCAATG AGGAATTCCC TGAAGCTGAA GAAAACAGTA CAAGAGAGGA GCTGAAGGAG 
GAGAAAGATG AGAGGGACCA AAAGGAAGAA GAAATCCCAG AAAATGCAAA CACTGAAAAG 
ATTTCCATGG AAACACTGCT CAAAGTGTTT GGAGGAGGAA ATGAAGTGCT GGACGCTAAC 
AGATTTGCCA GCTACCTAAA GACAGAGAAT ATTTACTCAG AGAACCTCAT CAAGACGTTC 
CAGGACCTGG GTGCCAAGAA CCTAGAGCCA ATCGAAGTTA ATATTCTCTT GAAGCATCCT 
TACATTCAGG ACCTGATTAC GAATTATGTA GACTATAAAA TCCCTGACAT TAAAATGATC 
CTTCAAAGGA GTGAGCATGC ACAAGGCAGT GATGGAGAAA GGTCACCCTC AAGACTTACA 
GACGAAAAGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Biology of reproduction85(4)690-701(2011 Oct)
Sironen A,Kotaja N,Mulhern H,Wyatt TA,Sisson JH,Pavlik JA,Miiluniemi M,Fleming MD,Lee L

Expression of SPEF2 during mouse spermatogenesis and identification of IFT20 as an interacting protein.
Biology of reproduction82(3)580-90(2010 Mar)
Sironen A,Hansen J,Thomsen B,Andersson M,Vilkki J,Toppari J,Kotaja N

Cloning and characterization of KPL2, a novel gene induced during ciliogenesis of tracheal epithelial cells.
American journal of respiratory cell and molecular biology20(4)675-83(1999 Apr)
Ostrowski LE,Andrews K,Potdar P,Matsuura H,Jetten A,Nettesheim P

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Spef2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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