hcfc1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hcfc1b
Entrez Gene ID	565206
Full Name	host cell factor C1b
Synonyms	fc07b09,wu:fc07b09,wu:fe17f08
General protein information	Preferred Names host cell factor C1b Names host cell factor 1 fe17f08
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005166699.4	XP_005166756.1	host cell factor 1 isoform X2
XM_005166699.3	XP_005166756.1	host cell factor 1 isoform X1
NM_001128537.1	NP_001122009.1	host cell factor 1
XM_021478282.1	XP_021333957.1	host cell factor 1 isoform X1
XM_021478283.1	XP_021333958.1	host cell factor 1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HCFC1	XP_521330.3
Rattus norvegicus (Norway rat)	Hcfc1	NP_001132979.1
Mus musculus (house mouse)	Hcfc1	NP_032250.2
Danio rerio (zebrafish)	hcfc1b	NP_001122009.1
Bos taurus (cattle)	HCFC1	XP_002707954.1
Homo sapiens (human)	HCFC1	NP_005325.2
Canis lupus familiaris (dog)	HCFC1	XP_005642062.1
Caenorhabditis elegans (roundworm)	hcf-1	NP_501279.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Hcfc1b, a zebrafish ortholog of HCFC1, regulates craniofacial development by modulating mmachc expression.
Quintana AM, Geiger EA, Achilly N, Rosenblatt DS, Maclean KN, Stabler SP, Artinger KB, Appel B, Shaikh TH
Developmental biology396(1)94-106(2014 Dec)

The first mecp2-null zebrafish model shows altered motor behaviors.
Pietri T, Roman AC, Guyon N, Romano SA, Washbourne P, Moens CB, de Polavieja GG, Sumbre G
Frontiers in neural circuits7118(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HCFC1 plays a role in craniofacial development, which is in part mediated through the regulation of MMACHC expression
Title: Hcfc1b, a zebrafish ortholog of HCFC1, regulates craniofacial development by modulating mmachc expression.

The following hcfc1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hcfc1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13857	NM_001128537.1 Latest version!	Danio rerio host cell factor C1b (hcfc1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa13857	XM_021478282.1 Latest version!	Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa13858	XM_005166699.4 Latest version!	Danio rerio host cell factor C1b (hcfc1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa143704	XM_021478283.1 Latest version!	Danio rerio host cell factor C1b (hcfc1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa13858	XM_005166699.3	Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hcfc1b ( NM_001128537.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122009.1 Danio rerio host cell factor C1b (hcfc1b), mRNA.

Price & Availability↑

CloneID	ODa13857
Clone ID Related Accession (Same CDS sequence)	NM_001128537.1 , XM_021478282.1
Accession Version	NM_001128537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	host cell factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571687.17. On Jul 31, 2008 this sequence version replaced XM_688491.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC CGACACAGGC CTCATCTTCA TTAGGTGCTG GACAGAGTGG CACTGTGCAA 
AGGGTGTGTT CTAACCCTCC TTGTGAAACA CATGAGACTG GCACCACCAA CACGCAGACC 
CAGGCTTCTT CTACTTTGGG TGCTGGACAG AGTGGTGCTG TGCAAAGGGT GTGTTCCAAC 
CCTCGTGAAA CGCATGAAAC GGGCACCACC AACACGCCGA CACAGGCCTC ATCTGGTATT 
GGCGCTGGAC AGAATGGCAC CGTGCAAATG GTCTGTTCCA ATCCTCCCTG TGAAACCCAC 
GAGACGGGCA CTACCAACAC AGCCACCACT GCTACAAGCA ACTTAGAAAC TGGAGAAGGA 
GCAGCTCAGC AGGCTGGGGA TGGACAGAGT GAAAGCGCCA CCAGCACAGA GCCCACATCA 
TCTACAGAAC CAGCCAATCC CGCGACACAA AGCAGAGCCA TCACCACAGT AACACAGGCA 
ACGCCCACTC CAGGACCATC AGTACCGGAG ATTTCCTCCA TGGCTGGATC TGCAGTAGTA 
TCTGAAACTC CAGGTGAGGC TGAGGCGATG GAGCAGGCCA GTGCAGAAGC CGTGGCGGCG 
GGTGAAGAGC CTATGCAGAC GGAGAGTCAG GGAGAGCTGA GTGAAGAGGG AGCCGTCAGG 
GTCGTCGCCA TTGATGAAAG TGCAGCAGGT GACGGGAGCA CTGTGCTTCA GGTTCACGTC 
GCCATGGAGG CACAGCCGGG GCAAGGAGAT CAGGCTGAGC AGATGGAGGC CGGTGTGGAG 
GCAGCAGAAG CCATGAGTCT CGCTGCTCAG GACTCAGAAG CCCTCGCCCT GCCACAGGAG 
CTGATGTCTG CAGAAGGACA GCAAACCACC CTGATGGTGA CTGGACTGAC CCCTGAGGAG 
CTGGCAGTGA CGGCGGCGGC TGAGGCAGCT GCACAGGCTG CAGCCACAGA AGAAGCCCAG 
GCCCTCGCTA TCCAGGCCGT CCTGCAGGCG GCTCAGCAGG CGGTGCTCAG TGGGGAAGGA 
GACGCAGGTC ACGACGGCCA GCAGGCCACC ACCATCCCTA TCGTTTTGAC TCAGCAGGAA 
CTTGCAGCTC TGGTCCAGCA GCAGCAGCAG CTTCAGGAGG CGCAGGCGGC CGCAGCACAG 
CAGTTAAGTG CTGAAGCCGC TGCCGCTGCT CTGCCCACTG AAGGTTTGGC ACCAGCAGAC 
AGCCTTAATG ACCCAGCGTC TGAGAGCAAC GGCCATGAGA TGCCAACCGC TGTGACCGGA 
GCTGTGGTTC GCCTCCTTCC ACGCACTTCT GCAGAGACTC TAGCCCCGTC AAGTACTTTT 
GCGCCGTCTC AGCCATTGGT GGTTGCCAGC CCTGCCAAGA TGCAAGCGGC CACTGCTTTA 
GCTGAGGTGG CCAACGGGAT CGAATCTGCT GCTGGGAAGC AAGAAGCACC TCCTGTAGTC 
GTAAAGCCTC CAGTGAAAAA AGAGAACCAG TGGTTTGATG TTGGTATCGT TAAAGTCACA 
AACATGGTCG TCACTCACTA CTACGTGCCA GCAGACGATT CAGCAGTGAC TGATGACGAT 
TCTGGCGCAA TCCCAGACTA CAGCCGAATG AAGAAAATCG AGTTGTCTCC GGGCACTGCT 
TACAAGTTCC GTGTGGCCGG TATCAACGCA TGCGGCCGAG GGACTTTCTC TGAGGTCTCT 
GCATTCAAGA CCTGCTTACC GGGATTCCCT GGAGCACCGT GTGCCATCAA AATCAGCAAG 
AGTCCCGATG GTGCTCACTT GACCTGGGAG CCTCCTTCAG TCACATCGGG GAAAATCATC 
GAGTATTCGG TGTATCTGGC CATCCAGAGC TCACAGACAG TCGAGGCAAA AGCGTCCGCT 
CCAGCTCAGC TGGCCTTTAT GCGGGTGTAC TGCGGACCCA ACCCGTCCTG CCTAGTGCAA 
TCGTCCAGCC TCTCCAATGC CCACATCGAC TACACCACCA AACCCGCTAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGTTACGGC CCTGCCACGC AAGTCAGGTG GCTACAAGAA 
ACTAGTAAAG ATGGCTCTGC GGCCAAACCT GCAGCAAAGA GGCCGGTGTC ATCACCTGAT 
ATGAAAGGCG CTGGTCCAAA GAAGGCCAGG TCTGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122009.1
CDS	196..6177
Translation	MASSGTSVLQPRWKRVLGWSGPVPRPRHGHRAVAIKELMVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKPKAPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYTLELRPGSNVAGWDIPITYGVLPPPRESHTAVVYTEKTSKKSRLIIYGGMSGCRLGDLWTLDIDTLTWNKPAISGAAPLPRSLHSATTITNKMFVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDSMSWETILMDTLEDNIPRARAGHCSVAINNRLYVWSGRDGYRKAWNNQVCCKDLWYLETDRPQPPSRVQLVRANTNSLEVSWGAVPTADTYLLQLQKYDIPAATAATSPTVNPMPSVPVNSPKSPVPAAAAPAAQSMPLSGVTLVPQVPSPTTAMPTSPLAASPRGPAILKVAAPHSTPGTSVVTVRQATPGAKSPVTVTSLPAGVRMVVPAQSTQGTPIGSSPQMSGMAALAAAAAATQKIPPSSATTVLNVPAGATLVKTVAVTPGSTTLPATVKVASPVMVTNPATRMLKTAAAQVGTSAISTPNTPTRPIITVHKSGTVTVAQQAQVVTTMVGGVTKTITLVKSPITMGGSGALYSQLSSLGKVMSVVQTKPVQTSAVTGQAGSSPVTLIQTKTPLPAGTILKLVTSADGKPTTIITTSQAGGTGTKPTILGISSVSPTTTNKPGTTIIKTIPMSALQGATGVTSSAGIKSPITIITTKVVTPGTGTPGKIITAVPKLTGAGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVSPGTVSADKPTVTTLVVKGTTGVTTLGTVTGTVSTSVAGANVANASLATPVTTLASIATLSSQVINPTAITVSAAQTNLATATIQSASQPTQVTLITTPSGAEAQPGQDLPVSFLASPTSEQPSTTGADAGDSAGDGATTVTLVCSNPPCETHETGTTNTATTASAKIGTEQICSNPPCETHVTGTTNTSTTASANMGRAQQVCTNPPSETHDTGTTNTATTASCNMGSTEMGTVNSKPGSSSPPASSSSEPSTPVSESGAEAGVMRPENLRTGTTYTSTTARSNMGSDQTGTVQSSNKSQAAISSTMMPVSSSESPGDTDTSDVSGSVCSNPPCETRETGTTSTPTQSSADMESGQSGSVQRVCSNPPCETHETGTTNTSTQASSSMAAGQSGTAQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTQTQASSTLGAGQSGAVQRVCSNPRETHETGTTNTPTQASSGIGAGQNGTVQMVCSNPPCETHETGTTNTATTATSNLETGEGAAQQAGDGQSESATSTEPTSSTEPANPATQSRAITTVTQATPTPGPSVPEISSMAGSAVVSETPGEAEAMEQASAEAVAAGEEPMQTESQGELSEEGAVRVVAIDESAAGDGSTVLQVHVAMEAQPGQGDQAEQMEAGVEAAEAMSLAAQDSEALALPQELMSAEGQQTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVLSGEGDAGHDGQQATTIPIVLTQQELAALVQQQQQLQEAQAAAAQQLSAEAAAAALPTEGLAPADSLNDPASESNGHEMPTAVTGAVVRLLPRTSAETLAPSSTFAPSQPLVVASPAKMQAATALAEVANGIESAAGKQEAPPVVVKPPVKKENQWFDVGIVKVTNMVVTHYYVPADDSAVTDDDSGAIPDYSRMKKIELSPGTAYKFRVAGINACGRGTFSEVSAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQTVEAKASAPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDGSAAKPAAKRPVSSPDMKGAGPKKARSDQ

Target ORF information:

RefSeq Version	NM_001128537.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio host cell factor C1b (hcfc1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128537.1

ORF Insert Sequence:

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC CGACACAGGC CTCATCTTCA TTAGGTGCTG GACAGAGTGG CACTGTGCAA 
AGGGTGTGTT CTAACCCTCC TTGTGAAACA CATGAGACTG GCACCACCAA CACGCAGACC 
CAGGCTTCTT CTACTTTGGG TGCTGGACAG AGTGGTGCTG TGCAAAGGGT GTGTTCCAAC 
CCTCGTGAAA CGCATGAAAC GGGCACCACC AACACGCCGA CACAGGCCTC ATCTGGTATT 
GGCGCTGGAC AGAATGGCAC CGTGCAAATG GTCTGTTCCA ATCCTCCCTG TGAAACCCAC 
GAGACGGGCA CTACCAACAC AGCCACCACT GCTACAAGCA ACTTAGAAAC TGGAGAAGGA 
GCAGCTCAGC AGGCTGGGGA TGGACAGAGT GAAAGCGCCA CCAGCACAGA GCCCACATCA 
TCTACAGAAC CAGCCAATCC CGCGACACAA AGCAGAGCCA TCACCACAGT AACACAGGCA 
ACGCCCACTC CAGGACCATC AGTACCGGAG ATTTCCTCCA TGGCTGGATC TGCAGTAGTA 
TCTGAAACTC CAGGTGAGGC TGAGGCGATG GAGCAGGCCA GTGCAGAAGC CGTGGCGGCG 
GGTGAAGAGC CTATGCAGAC GGAGAGTCAG GGAGAGCTGA GTGAAGAGGG AGCCGTCAGG 
GTCGTCGCCA TTGATGAAAG TGCAGCAGGT GACGGGAGCA CTGTGCTTCA GGTTCACGTC 
GCCATGGAGG CACAGCCGGG GCAAGGAGAT CAGGCTGAGC AGATGGAGGC CGGTGTGGAG 
GCAGCAGAAG CCATGAGTCT CGCTGCTCAG GACTCAGAAG CCCTCGCCCT GCCACAGGAG 
CTGATGTCTG CAGAAGGACA GCAAACCACC CTGATGGTGA CTGGACTGAC CCCTGAGGAG 
CTGGCAGTGA CGGCGGCGGC TGAGGCAGCT GCACAGGCTG CAGCCACAGA AGAAGCCCAG 
GCCCTCGCTA TCCAGGCCGT CCTGCAGGCG GCTCAGCAGG CGGTGCTCAG TGGGGAAGGA 
GACGCAGGTC ACGACGGCCA GCAGGCCACC ACCATCCCTA TCGTTTTGAC TCAGCAGGAA 
CTTGCAGCTC TGGTCCAGCA GCAGCAGCAG CTTCAGGAGG CGCAGGCGGC CGCAGCACAG 
CAGTTAAGTG CTGAAGCCGC TGCCGCTGCT CTGCCCACTG AAGGTTTGGC ACCAGCAGAC 
AGCCTTAATG ACCCAGCGTC TGAGAGCAAC GGCCATGAGA TGCCAACCGC TGTGACCGGA 
GCTGTGGTTC GCCTCCTTCC ACGCACTTCT GCAGAGACTC TAGCCCCGTC AAGTACTTTT 
GCGCCGTCTC AGCCATTGGT GGTTGCCAGC CCTGCCAAGA TGCAAGCGGC CACTGCTTTA 
GCTGAGGTGG CCAACGGGAT CGAATCTGCT GCTGGGAAGC AAGAAGCACC TCCTGTAGTC 
GTAAAGCCTC CAGTGAAAAA AGAGAACCAG TGGTTTGATG TTGGTATCGT TAAAGTCACA 
AACATGGTCG TCACTCACTA CTACGTGCCA GCAGACGATT CAGCAGTGAC TGATGACGAT 
TCTGGCGCAA TCCCAGACTA CAGCCGAATG AAGAAAATCG AGTTGTCTCC GGGCACTGCT 
TACAAGTTCC GTGTGGCCGG TATCAACGCA TGCGGCCGAG GGACTTTCTC TGAGGTCTCT 
GCATTCAAGA CCTGCTTACC GGGATTCCCT GGAGCACCGT GTGCCATCAA AATCAGCAAG 
AGTCCCGATG GTGCTCACTT GACCTGGGAG CCTCCTTCAG TCACATCGGG GAAAATCATC 
GAGTATTCGG TGTATCTGGC CATCCAGAGC TCACAGACAG TCGAGGCAAA AGCGTCCGCT 
CCAGCTCAGC TGGCCTTTAT GCGGGTGTAC TGCGGACCCA ACCCGTCCTG CCTAGTGCAA 
TCGTCCAGCC TCTCCAATGC CCACATCGAC TACACCACCA AACCCGCTAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGTTACGGC CCTGCCACGC AAGTCAGGTG GCTACAAGAA 
ACTAGTAAAG ATGGCTCTGC GGCCAAACCT GCAGCAAAGA GGCCGGTGTC ATCACCTGAT 
ATGAAAGGCG CTGGTCCAAA GAAGGCCAGG TCTGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcfc1b ( XM_021478282.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333957.1 Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.

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CloneID	ODa13857
Clone ID Related Accession (Same CDS sequence)	NM_001128537.1 , XM_021478282.1
Accession Version	XM_021478282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	host cell factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC CGACACAGGC CTCATCTTCA TTAGGTGCTG GACAGAGTGG CACTGTGCAA 
AGGGTGTGTT CTAACCCTCC TTGTGAAACA CATGAGACTG GCACCACCAA CACGCAGACC 
CAGGCTTCTT CTACTTTGGG TGCTGGACAG AGTGGTGCTG TGCAAAGGGT GTGTTCCAAC 
CCTCGTGAAA CGCATGAAAC GGGCACCACC AACACGCCGA CACAGGCCTC ATCTGGTATT 
GGCGCTGGAC AGAATGGCAC CGTGCAAATG GTCTGTTCCA ATCCTCCCTG TGAAACCCAC 
GAGACGGGCA CTACCAACAC AGCCACCACT GCTACAAGCA ACTTAGAAAC TGGAGAAGGA 
GCAGCTCAGC AGGCTGGGGA TGGACAGAGT GAAAGCGCCA CCAGCACAGA GCCCACATCA 
TCTACAGAAC CAGCCAATCC CGCGACACAA AGCAGAGCCA TCACCACAGT AACACAGGCA 
ACGCCCACTC CAGGACCATC AGTACCGGAG ATTTCCTCCA TGGCTGGATC TGCAGTAGTA 
TCTGAAACTC CAGGTGAGGC TGAGGCGATG GAGCAGGCCA GTGCAGAAGC CGTGGCGGCG 
GGTGAAGAGC CTATGCAGAC GGAGAGTCAG GGAGAGCTGA GTGAAGAGGG AGCCGTCAGG 
GTCGTCGCCA TTGATGAAAG TGCAGCAGGT GACGGGAGCA CTGTGCTTCA GGTTCACGTC 
GCCATGGAGG CACAGCCGGG GCAAGGAGAT CAGGCTGAGC AGATGGAGGC CGGTGTGGAG 
GCAGCAGAAG CCATGAGTCT CGCTGCTCAG GACTCAGAAG CCCTCGCCCT GCCACAGGAG 
CTGATGTCTG CAGAAGGACA GCAAACCACC CTGATGGTGA CTGGACTGAC CCCTGAGGAG 
CTGGCAGTGA CGGCGGCGGC TGAGGCAGCT GCACAGGCTG CAGCCACAGA AGAAGCCCAG 
GCCCTCGCTA TCCAGGCCGT CCTGCAGGCG GCTCAGCAGG CGGTGCTCAG TGGGGAAGGA 
GACGCAGGTC ACGACGGCCA GCAGGCCACC ACCATCCCTA TCGTTTTGAC TCAGCAGGAA 
CTTGCAGCTC TGGTCCAGCA GCAGCAGCAG CTTCAGGAGG CGCAGGCGGC CGCAGCACAG 
CAGTTAAGTG CTGAAGCCGC TGCCGCTGCT CTGCCCACTG AAGGTTTGGC ACCAGCAGAC 
AGCCTTAATG ACCCAGCGTC TGAGAGCAAC GGCCATGAGA TGCCAACCGC TGTGACCGGA 
GCTGTGGTTC GCCTCCTTCC ACGCACTTCT GCAGAGACTC TAGCCCCGTC AAGTACTTTT 
GCGCCGTCTC AGCCATTGGT GGTTGCCAGC CCTGCCAAGA TGCAAGCGGC CACTGCTTTA 
GCTGAGGTGG CCAACGGGAT CGAATCTGCT GCTGGGAAGC AAGAAGCACC TCCTGTAGTC 
GTAAAGCCTC CAGTGAAAAA AGAGAACCAG TGGTTTGATG TTGGTATCGT TAAAGTCACA 
AACATGGTCG TCACTCACTA CTACGTGCCA GCAGACGATT CAGCAGTGAC TGATGACGAT 
TCTGGCGCAA TCCCAGACTA CAGCCGAATG AAGAAAATCG AGTTGTCTCC GGGCACTGCT 
TACAAGTTCC GTGTGGCCGG TATCAACGCA TGCGGCCGAG GGACTTTCTC TGAGGTCTCT 
GCATTCAAGA CCTGCTTACC GGGATTCCCT GGAGCACCGT GTGCCATCAA AATCAGCAAG 
AGTCCCGATG GTGCTCACTT GACCTGGGAG CCTCCTTCAG TCACATCGGG GAAAATCATC 
GAGTATTCGG TGTATCTGGC CATCCAGAGC TCACAGACAG TCGAGGCAAA AGCGTCCGCT 
CCAGCTCAGC TGGCCTTTAT GCGGGTGTAC TGCGGACCCA ACCCGTCCTG CCTAGTGCAA 
TCGTCCAGCC TCTCCAATGC CCACATCGAC TACACCACCA AACCCGCTAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGTTACGGC CCTGCCACGC AAGTCAGGTG GCTACAAGAA 
ACTAGTAAAG ATGGCTCTGC GGCCAAACCT GCAGCAAAGA GGCCGGTGTC ATCACCTGAT 
ATGAAAGGCG CTGGTCCAAA GAAGGCCAGG TCTGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333957.1
CDS	226..6207
Translation	MASSGTSVLQPRWKRVLGWSGPVPRPRHGHRAVAIKELMVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKPKAPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYTLELRPGSNVAGWDIPITYGVLPPPRESHTAVVYTEKTSKKSRLIIYGGMSGCRLGDLWTLDIDTLTWNKPAISGAAPLPRSLHSATTITNKMFVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDSMSWETILMDTLEDNIPRARAGHCSVAINNRLYVWSGRDGYRKAWNNQVCCKDLWYLETDRPQPPSRVQLVRANTNSLEVSWGAVPTADTYLLQLQKYDIPAATAATSPTVNPMPSVPVNSPKSPVPAAAAPAAQSMPLSGVTLVPQVPSPTTAMPTSPLAASPRGPAILKVAAPHSTPGTSVVTVRQATPGAKSPVTVTSLPAGVRMVVPAQSTQGTPIGSSPQMSGMAALAAAAAATQKIPPSSATTVLNVPAGATLVKTVAVTPGSTTLPATVKVASPVMVTNPATRMLKTAAAQVGTSAISTPNTPTRPIITVHKSGTVTVAQQAQVVTTMVGGVTKTITLVKSPITMGGSGALYSQLSSLGKVMSVVQTKPVQTSAVTGQAGSSPVTLIQTKTPLPAGTILKLVTSADGKPTTIITTSQAGGTGTKPTILGISSVSPTTTNKPGTTIIKTIPMSALQGATGVTSSAGIKSPITIITTKVVTPGTGTPGKIITAVPKLTGAGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVSPGTVSADKPTVTTLVVKGTTGVTTLGTVTGTVSTSVAGANVANASLATPVTTLASIATLSSQVINPTAITVSAAQTNLATATIQSASQPTQVTLITTPSGAEAQPGQDLPVSFLASPTSEQPSTTGADAGDSAGDGATTVTLVCSNPPCETHETGTTNTATTASAKIGTEQICSNPPCETHVTGTTNTSTTASANMGRAQQVCTNPPSETHDTGTTNTATTASCNMGSTEMGTVNSKPGSSSPPASSSSEPSTPVSESGAEAGVMRPENLRTGTTYTSTTARSNMGSDQTGTVQSSNKSQAAISSTMMPVSSSESPGDTDTSDVSGSVCSNPPCETRETGTTSTPTQSSADMESGQSGSVQRVCSNPPCETHETGTTNTSTQASSSMAAGQSGTAQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTQTQASSTLGAGQSGAVQRVCSNPRETHETGTTNTPTQASSGIGAGQNGTVQMVCSNPPCETHETGTTNTATTATSNLETGEGAAQQAGDGQSESATSTEPTSSTEPANPATQSRAITTVTQATPTPGPSVPEISSMAGSAVVSETPGEAEAMEQASAEAVAAGEEPMQTESQGELSEEGAVRVVAIDESAAGDGSTVLQVHVAMEAQPGQGDQAEQMEAGVEAAEAMSLAAQDSEALALPQELMSAEGQQTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVLSGEGDAGHDGQQATTIPIVLTQQELAALVQQQQQLQEAQAAAAQQLSAEAAAAALPTEGLAPADSLNDPASESNGHEMPTAVTGAVVRLLPRTSAETLAPSSTFAPSQPLVVASPAKMQAATALAEVANGIESAAGKQEAPPVVVKPPVKKENQWFDVGIVKVTNMVVTHYYVPADDSAVTDDDSGAIPDYSRMKKIELSPGTAYKFRVAGINACGRGTFSEVSAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQTVEAKASAPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDGSAAKPAAKRPVSSPDMKGAGPKKARSDQ

Target ORF information:

RefSeq Version	XM_021478282.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478282.1

ORF Insert Sequence:

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC CGACACAGGC CTCATCTTCA TTAGGTGCTG GACAGAGTGG CACTGTGCAA 
AGGGTGTGTT CTAACCCTCC TTGTGAAACA CATGAGACTG GCACCACCAA CACGCAGACC 
CAGGCTTCTT CTACTTTGGG TGCTGGACAG AGTGGTGCTG TGCAAAGGGT GTGTTCCAAC 
CCTCGTGAAA CGCATGAAAC GGGCACCACC AACACGCCGA CACAGGCCTC ATCTGGTATT 
GGCGCTGGAC AGAATGGCAC CGTGCAAATG GTCTGTTCCA ATCCTCCCTG TGAAACCCAC 
GAGACGGGCA CTACCAACAC AGCCACCACT GCTACAAGCA ACTTAGAAAC TGGAGAAGGA 
GCAGCTCAGC AGGCTGGGGA TGGACAGAGT GAAAGCGCCA CCAGCACAGA GCCCACATCA 
TCTACAGAAC CAGCCAATCC CGCGACACAA AGCAGAGCCA TCACCACAGT AACACAGGCA 
ACGCCCACTC CAGGACCATC AGTACCGGAG ATTTCCTCCA TGGCTGGATC TGCAGTAGTA 
TCTGAAACTC CAGGTGAGGC TGAGGCGATG GAGCAGGCCA GTGCAGAAGC CGTGGCGGCG 
GGTGAAGAGC CTATGCAGAC GGAGAGTCAG GGAGAGCTGA GTGAAGAGGG AGCCGTCAGG 
GTCGTCGCCA TTGATGAAAG TGCAGCAGGT GACGGGAGCA CTGTGCTTCA GGTTCACGTC 
GCCATGGAGG CACAGCCGGG GCAAGGAGAT CAGGCTGAGC AGATGGAGGC CGGTGTGGAG 
GCAGCAGAAG CCATGAGTCT CGCTGCTCAG GACTCAGAAG CCCTCGCCCT GCCACAGGAG 
CTGATGTCTG CAGAAGGACA GCAAACCACC CTGATGGTGA CTGGACTGAC CCCTGAGGAG 
CTGGCAGTGA CGGCGGCGGC TGAGGCAGCT GCACAGGCTG CAGCCACAGA AGAAGCCCAG 
GCCCTCGCTA TCCAGGCCGT CCTGCAGGCG GCTCAGCAGG CGGTGCTCAG TGGGGAAGGA 
GACGCAGGTC ACGACGGCCA GCAGGCCACC ACCATCCCTA TCGTTTTGAC TCAGCAGGAA 
CTTGCAGCTC TGGTCCAGCA GCAGCAGCAG CTTCAGGAGG CGCAGGCGGC CGCAGCACAG 
CAGTTAAGTG CTGAAGCCGC TGCCGCTGCT CTGCCCACTG AAGGTTTGGC ACCAGCAGAC 
AGCCTTAATG ACCCAGCGTC TGAGAGCAAC GGCCATGAGA TGCCAACCGC TGTGACCGGA 
GCTGTGGTTC GCCTCCTTCC ACGCACTTCT GCAGAGACTC TAGCCCCGTC AAGTACTTTT 
GCGCCGTCTC AGCCATTGGT GGTTGCCAGC CCTGCCAAGA TGCAAGCGGC CACTGCTTTA 
GCTGAGGTGG CCAACGGGAT CGAATCTGCT GCTGGGAAGC AAGAAGCACC TCCTGTAGTC 
GTAAAGCCTC CAGTGAAAAA AGAGAACCAG TGGTTTGATG TTGGTATCGT TAAAGTCACA 
AACATGGTCG TCACTCACTA CTACGTGCCA GCAGACGATT CAGCAGTGAC TGATGACGAT 
TCTGGCGCAA TCCCAGACTA CAGCCGAATG AAGAAAATCG AGTTGTCTCC GGGCACTGCT 
TACAAGTTCC GTGTGGCCGG TATCAACGCA TGCGGCCGAG GGACTTTCTC TGAGGTCTCT 
GCATTCAAGA CCTGCTTACC GGGATTCCCT GGAGCACCGT GTGCCATCAA AATCAGCAAG 
AGTCCCGATG GTGCTCACTT GACCTGGGAG CCTCCTTCAG TCACATCGGG GAAAATCATC 
GAGTATTCGG TGTATCTGGC CATCCAGAGC TCACAGACAG TCGAGGCAAA AGCGTCCGCT 
CCAGCTCAGC TGGCCTTTAT GCGGGTGTAC TGCGGACCCA ACCCGTCCTG CCTAGTGCAA 
TCGTCCAGCC TCTCCAATGC CCACATCGAC TACACCACCA AACCCGCTAT CATCTTCCGC 
ATCGCCGCCC GCAATGAGAA GGGTTACGGC CCTGCCACGC AAGTCAGGTG GCTACAAGAA 
ACTAGTAAAG ATGGCTCTGC GGCCAAACCT GCAGCAAAGA GGCCGGTGTC ATCACCTGAT 
ATGAAAGGCG CTGGTCCAAA GAAGGCCAGG TCTGACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcfc1b ( XM_005166699.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166756.1 Danio rerio host cell factor C1b (hcfc1b), transcript variant X2, mRNA.

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CloneID	ODa13858
Clone ID Related Accession (Same CDS sequence)	XM_005166699.4 , XM_005166699.3
Accession Version	XM_005166699.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	host cell factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166699.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGCGTG 
ACTAGCAGTG CTGGCATCAA GTCCCCCATC ACAATTATCA CCACTAAGGT TGTTACTCCT 
GGCACAGGGA CTCCAGGGAA AATCATCACA GCTGTGCCTA AGCTGACAGG TGCAGGACAA 
CAGGGAGTCA CACAGGTGGT CCTGAAGGGA GCGCCAGGGC AGCCCGGCAC CATCCTGCGC 
ACTGTGCCTA TGGGTGGTGT GCGGCTGGTT TCACCAGGCA CTGTGTCTGC CGACAAACCA 
ACTGTTACCA CACTGGTCGT CAAGGGCACC ACAGGAGTCA CGACTCTGGG TACAGTAACT 
GGAACTGTAT CAACTAGCGT TGCAGGAGCC AATGTTGCTA ATGCCAGCCT GGCGACTCCT 
GTCACCACAC TGGCCTCCAT CGCCACTTTA TCCAGCCAGG TGATCAATCC CACAGCCATC 
ACTGTATCTG CTGCCCAGAC CAACCTCGCC ACCGCTACAA TACAATCTGC ATCTCAGCCT 
ACTCAAGTAA CCCTGATCAC GACCCCGAGT GGTGCTGAAG CCCAGCCTGG GCAGGATCTC 
CCCGTCTCTT TCCTGGCCTC TCCCACATCA GAACAGCCCT CCACCACCGG TGCAGATGCT 
GGCGATAGCG CGGGTGATGG TGCCACCACC GTCACACTAG TATGCTCTAA CCCGCCCTGC 
GAGACCCACG AGACTGGAAC AACAAACACG GCTACTACAG CTTCTGCCAA AATAGGCACT 
GAACAGATTT GTTCCAACCC TCCCTGTGAA ACGCACGTAA CGGGCACCAC CAACACCTCA 
ACCACCGCCT CTGCAAACAT GGGCAGGGCA CAGCAGGTCT GCACCAACCC GCCATCAGAA 
ACCCATGATA CGGGCACCAC CAACACAGCC ACCACTGCCA GCTGTAATAT GGGCTCCACA 
GAAATGGGTA CCGTGAACAG CAAACCAGGC TCTTCATCGC CGCCTGCGTC CTCCTCTTCT 
GAACCATCGA CACCTGTCTC AGAGAGTGGA GCCGAAGCAG GCGTCATGCG TCCAGAAAAC 
CTCCGCACAG GCACCACATA CACCTCCACC ACCGCCCGCT CCAACATGGG CTCTGACCAG 
ACGGGAACAG TCCAGAGCTC CAATAAGAGC CAAGCCGCCA TCTCCTCTAC AATGATGCCA 
GTGAGCTCCA GCGAATCTCC CGGTGACACA GACACTTCTG ACGTCTCCGG CAGCGTCTGC 
TCAAATCCAC CTTGCGAGAC GCGTGAAACG GGCACCACTA GTACACCTAC ACAATCAAGC 
GCAGATATGG AGAGTGGACA GAGTGGTTCT GTGCAAAGGG TGTGTTCCAA TCCTCCTTGT 
GAAACACATG AGACTGGCAC CACCAACACG TCAACACAGG CATCGTCTTC AATGGCCGCT 
GGACAGAGTG GCACTGCGCA AAGGGTGTGT TCTAACCCTC CTTGTGAAAC ACATGAGACT 
GGCACCACCA ACACGCCGAC ACAGGCCTCA TCTTCATTAG GTGCTGGACA GAGTGGCACT 
GTGCAAAGGG TGTGTTCTAA CCCTCCTTGT GAAACACATG AGACTGGCAC CACCAACACG 
CCGACACAGG CCTCATCTTC ATTAGGTGCT GGACAGAGTG GCACTGTGCA AAGGGTGTGT 
TCTAACCCTC CTTGTGAAAC ACATGAGACT GGCACCACCA ACACGCAGAC CCAGGCTTCT 
TCTACTTTGG GTGCTGGACA GAGTGGTGCT GTGCAAAGGG TGTGTTCCAA CCCTCGTGAA 
ACGCATGAAA CGGGCACCAC CAACACGCCG ACACAGGCCT CATCTGGTAT TGGCGCTGGA 
CAGAATGGCA CCGTGCAAAT GGTCTGTTCC AATCCTCCCT GTGAAACCCA CGAGACGGGC 
ACTACCAACA CAGCCACCAC TGCTACAAGC AACTTAGAAA CTGGAGAAGG AGCAGCTCAG 
CAGGCTGGGG ATGGACAGAG TGAAAGCGCC ACCAGCACAG AGCCCACATC ATCTACAGAA 
CCAGCCAATC CCGCGACACA AAGCAGAGCC ATCACCACAG TAACACAGGC AACGCCCACT 
CCAGGACCAT CAGTACCGGA GATTTCCTCC ATGGCTGGAT CTGCAGTAGT ATCTGAAACT 
CCAGGTGAGG CTGAGGCGAT GGAGCAGGCC AGTGCAGAAG CCGTGGCGGC GGGTGAAGAG 
CCTATGCAGA CGGAGAGTCA GGGAGAGCTG AGTGAAGAGG GAGCCGTCAG GGTCGTCGCC 
ATTGATGAAA GTGCAGCAGG TGACGGGAGC ACTGTGCTTC AGGTTCACGT CGCCATGGAG 
GCACAGCCGG GGCAAGGAGA TCAGGCTGAG CAGATGGAGG CCGGTGTGGA GGCAGCAGAA 
GCCATGAGTC TCGCTGCTCA GGACTCAGAA GCCCTCGCCC TGCCACAGGA GCTGATGTCT 
GCAGAAGGAC AGCAAACCAC CCTGATGGTG ACTGGACTGA CCCCTGAGGA GCTGGCAGTG 
ACGGCGGCGG CTGAGGCAGC TGCACAGGCT GCAGCCACAG AAGAAGCCCA GGCCCTCGCT 
ATCCAGGCCG TCCTGCAGGC GGCTCAGCAG GCGGTGCTCA GTGGGGAAGG AGACGCAGGT 
CACGACGGCC AGCAGGCCAC CACCATCCCT ATCGTTTTGA CTCAGCAGGA ACTTGCAGCT 
CTGGTCCAGC AGCAGCAGCA GCTTCAGGAG GCGCAGGCGG CCGCAGCACA GCAGTTAAGT 
GCTGAAGCCG CTGCCGCTGC TCTGCCCACT GAAGGTTTGG CACCAGCAGA CAGCCTTAAT 
GACCCAGCGT CTGAGAGCAA CGGCCATGAG ATGCCAACCG CTGTGACCGG AGCTGTGGTT 
CGCCTCCTTC CACGCACTTC TGCAGAGACT CTAGCCCCGT CAAGTACTTT TGCGCCGTCT 
CAGCCATTGG TGGTTGCCAG CCCTGCCAAG ATGCAAGCGG CCACTGCTTT AGCTGAGGTG 
GCCAACGGGA TCGAATCTGC TGCTGGGAAG CAAGAAGCAC CTCCTGTAGT CGTAAAGCCT 
CCAGTGAAAA AAGAGAACCA GTGGTTTGAT GTTGGTATCG TTAAAGTCAC AAACATGGTC 
GTCACTCACT ACTACGTGCC AGCAGACGAT TCAGCAGTGA CTGATGACGA TTCTGGCGCA 
ATCCCAGACT ACAGCCGAAT GAAGAAAATC GAGTTGTCTC CGGGCACTGC TTACAAGTTC 
CGTGTGGCCG GTATCAACGC ATGCGGCCGA GGGACTTTCT CTGAGGTCTC TGCATTCAAG 
ACCTGCTTAC CGGGATTCCC TGGAGCACCG TGTGCCATCA AAATCAGCAA GAGTCCCGAT 
GGTGCTCACT TGACCTGGGA GCCTCCTTCA GTCACATCGG GGAAAATCAT CGAGTATTCG 
GTGTATCTGG CCATCCAGAG CTCACAGACA GTCGAGGCAA AAGCGTCCGC TCCAGCTCAG 
CTGGCCTTTA TGCGGGTGTA CTGCGGACCC AACCCGTCCT GCCTAGTGCA ATCGTCCAGC 
CTCTCCAATG CCCACATCGA CTACACCACC AAACCCGCTA TCATCTTCCG CATCGCCGCC 
CGCAATGAGA AGGGTTACGG CCCTGCCACG CAAGTCAGGT GGCTACAAGA AACTAGTAAA 
GATGGCTCTG CGGCCAAACC TGCAGCAAAG AGGCCGGTGT CATCACCTGA TATGAAAGGC 
GCTGGTCCAA AGAAGGCCAG GTCTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166756.1
CDS	191..6163
Translation	MASSGTSVLQPRWKRVLGWSGPVPRPRHGHRAVAIKELMVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKPKAPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYTLELRPGSNVAGWDIPITYGVLPPPRESHTAVVYTEKTSKKSRLIIYGGMSGCRLGDLWTLDIDTLTWNKPAISGAAPLPRSLHSATTITNKMFVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDSMSWETILMDTLEDNIPRARAGHCSVAINNRLYVWSGRDGYRKAWNNQVCCKDLWYLETDRPQPPSRVQLVRANTNSLEVSWGAVPTADTYLLQLQKYDIPAATAATSPTVNPMPSVPVNSPKSPVPAAAAPAAQSMPLSGVTLVPQVPSPTTAMPTSPLAASPRGPAILKVAAPHSTPGTSVVTVRQATPGAKSPVTVTSLPAGVRMVVPAQSTQGTPIGSSPQMSGMAALAAAAAATQKIPPSSATTVLNVPAGATLVKTVAVTPGSTTLPATVKVASPVMVTNPATRMLKTAAAQVGTSAISTPNTPTRPIITVHKSGTVTVAQQAQVVTTMVGGVTKTITLVKSPITMGGSGALYSQLSSLGKVMSVVQTKPVQTSAVTGQAGSSPVTLIQTKTPLPAGTILKLVTSADGKPTTIITTSQAGGTGTKPTILGISSVSPTTTNKPGTTIIKTIPMSALQGVTSSAGIKSPITIITTKVVTPGTGTPGKIITAVPKLTGAGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVSPGTVSADKPTVTTLVVKGTTGVTTLGTVTGTVSTSVAGANVANASLATPVTTLASIATLSSQVINPTAITVSAAQTNLATATIQSASQPTQVTLITTPSGAEAQPGQDLPVSFLASPTSEQPSTTGADAGDSAGDGATTVTLVCSNPPCETHETGTTNTATTASAKIGTEQICSNPPCETHVTGTTNTSTTASANMGRAQQVCTNPPSETHDTGTTNTATTASCNMGSTEMGTVNSKPGSSSPPASSSSEPSTPVSESGAEAGVMRPENLRTGTTYTSTTARSNMGSDQTGTVQSSNKSQAAISSTMMPVSSSESPGDTDTSDVSGSVCSNPPCETRETGTTSTPTQSSADMESGQSGSVQRVCSNPPCETHETGTTNTSTQASSSMAAGQSGTAQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTQTQASSTLGAGQSGAVQRVCSNPRETHETGTTNTPTQASSGIGAGQNGTVQMVCSNPPCETHETGTTNTATTATSNLETGEGAAQQAGDGQSESATSTEPTSSTEPANPATQSRAITTVTQATPTPGPSVPEISSMAGSAVVSETPGEAEAMEQASAEAVAAGEEPMQTESQGELSEEGAVRVVAIDESAAGDGSTVLQVHVAMEAQPGQGDQAEQMEAGVEAAEAMSLAAQDSEALALPQELMSAEGQQTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVLSGEGDAGHDGQQATTIPIVLTQQELAALVQQQQQLQEAQAAAAQQLSAEAAAAALPTEGLAPADSLNDPASESNGHEMPTAVTGAVVRLLPRTSAETLAPSSTFAPSQPLVVASPAKMQAATALAEVANGIESAAGKQEAPPVVVKPPVKKENQWFDVGIVKVTNMVVTHYYVPADDSAVTDDDSGAIPDYSRMKKIELSPGTAYKFRVAGINACGRGTFSEVSAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQTVEAKASAPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDGSAAKPAAKRPVSSPDMKGAGPKKARSDQ

Target ORF information:

RefSeq Version	XM_005166699.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio host cell factor C1b (hcfc1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166699.4

ORF Insert Sequence:

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGCGTG 
ACTAGCAGTG CTGGCATCAA GTCCCCCATC ACAATTATCA CCACTAAGGT TGTTACTCCT 
GGCACAGGGA CTCCAGGGAA AATCATCACA GCTGTGCCTA AGCTGACAGG TGCAGGACAA 
CAGGGAGTCA CACAGGTGGT CCTGAAGGGA GCGCCAGGGC AGCCCGGCAC CATCCTGCGC 
ACTGTGCCTA TGGGTGGTGT GCGGCTGGTT TCACCAGGCA CTGTGTCTGC CGACAAACCA 
ACTGTTACCA CACTGGTCGT CAAGGGCACC ACAGGAGTCA CGACTCTGGG TACAGTAACT 
GGAACTGTAT CAACTAGCGT TGCAGGAGCC AATGTTGCTA ATGCCAGCCT GGCGACTCCT 
GTCACCACAC TGGCCTCCAT CGCCACTTTA TCCAGCCAGG TGATCAATCC CACAGCCATC 
ACTGTATCTG CTGCCCAGAC CAACCTCGCC ACCGCTACAA TACAATCTGC ATCTCAGCCT 
ACTCAAGTAA CCCTGATCAC GACCCCGAGT GGTGCTGAAG CCCAGCCTGG GCAGGATCTC 
CCCGTCTCTT TCCTGGCCTC TCCCACATCA GAACAGCCCT CCACCACCGG TGCAGATGCT 
GGCGATAGCG CGGGTGATGG TGCCACCACC GTCACACTAG TATGCTCTAA CCCGCCCTGC 
GAGACCCACG AGACTGGAAC AACAAACACG GCTACTACAG CTTCTGCCAA AATAGGCACT 
GAACAGATTT GTTCCAACCC TCCCTGTGAA ACGCACGTAA CGGGCACCAC CAACACCTCA 
ACCACCGCCT CTGCAAACAT GGGCAGGGCA CAGCAGGTCT GCACCAACCC GCCATCAGAA 
ACCCATGATA CGGGCACCAC CAACACAGCC ACCACTGCCA GCTGTAATAT GGGCTCCACA 
GAAATGGGTA CCGTGAACAG CAAACCAGGC TCTTCATCGC CGCCTGCGTC CTCCTCTTCT 
GAACCATCGA CACCTGTCTC AGAGAGTGGA GCCGAAGCAG GCGTCATGCG TCCAGAAAAC 
CTCCGCACAG GCACCACATA CACCTCCACC ACCGCCCGCT CCAACATGGG CTCTGACCAG 
ACGGGAACAG TCCAGAGCTC CAATAAGAGC CAAGCCGCCA TCTCCTCTAC AATGATGCCA 
GTGAGCTCCA GCGAATCTCC CGGTGACACA GACACTTCTG ACGTCTCCGG CAGCGTCTGC 
TCAAATCCAC CTTGCGAGAC GCGTGAAACG GGCACCACTA GTACACCTAC ACAATCAAGC 
GCAGATATGG AGAGTGGACA GAGTGGTTCT GTGCAAAGGG TGTGTTCCAA TCCTCCTTGT 
GAAACACATG AGACTGGCAC CACCAACACG TCAACACAGG CATCGTCTTC AATGGCCGCT 
GGACAGAGTG GCACTGCGCA AAGGGTGTGT TCTAACCCTC CTTGTGAAAC ACATGAGACT 
GGCACCACCA ACACGCCGAC ACAGGCCTCA TCTTCATTAG GTGCTGGACA GAGTGGCACT 
GTGCAAAGGG TGTGTTCTAA CCCTCCTTGT GAAACACATG AGACTGGCAC CACCAACACG 
CCGACACAGG CCTCATCTTC ATTAGGTGCT GGACAGAGTG GCACTGTGCA AAGGGTGTGT 
TCTAACCCTC CTTGTGAAAC ACATGAGACT GGCACCACCA ACACGCAGAC CCAGGCTTCT 
TCTACTTTGG GTGCTGGACA GAGTGGTGCT GTGCAAAGGG TGTGTTCCAA CCCTCGTGAA 
ACGCATGAAA CGGGCACCAC CAACACGCCG ACACAGGCCT CATCTGGTAT TGGCGCTGGA 
CAGAATGGCA CCGTGCAAAT GGTCTGTTCC AATCCTCCCT GTGAAACCCA CGAGACGGGC 
ACTACCAACA CAGCCACCAC TGCTACAAGC AACTTAGAAA CTGGAGAAGG AGCAGCTCAG 
CAGGCTGGGG ATGGACAGAG TGAAAGCGCC ACCAGCACAG AGCCCACATC ATCTACAGAA 
CCAGCCAATC CCGCGACACA AAGCAGAGCC ATCACCACAG TAACACAGGC AACGCCCACT 
CCAGGACCAT CAGTACCGGA GATTTCCTCC ATGGCTGGAT CTGCAGTAGT ATCTGAAACT 
CCAGGTGAGG CTGAGGCGAT GGAGCAGGCC AGTGCAGAAG CCGTGGCGGC GGGTGAAGAG 
CCTATGCAGA CGGAGAGTCA GGGAGAGCTG AGTGAAGAGG GAGCCGTCAG GGTCGTCGCC 
ATTGATGAAA GTGCAGCAGG TGACGGGAGC ACTGTGCTTC AGGTTCACGT CGCCATGGAG 
GCACAGCCGG GGCAAGGAGA TCAGGCTGAG CAGATGGAGG CCGGTGTGGA GGCAGCAGAA 
GCCATGAGTC TCGCTGCTCA GGACTCAGAA GCCCTCGCCC TGCCACAGGA GCTGATGTCT 
GCAGAAGGAC AGCAAACCAC CCTGATGGTG ACTGGACTGA CCCCTGAGGA GCTGGCAGTG 
ACGGCGGCGG CTGAGGCAGC TGCACAGGCT GCAGCCACAG AAGAAGCCCA GGCCCTCGCT 
ATCCAGGCCG TCCTGCAGGC GGCTCAGCAG GCGGTGCTCA GTGGGGAAGG AGACGCAGGT 
CACGACGGCC AGCAGGCCAC CACCATCCCT ATCGTTTTGA CTCAGCAGGA ACTTGCAGCT 
CTGGTCCAGC AGCAGCAGCA GCTTCAGGAG GCGCAGGCGG CCGCAGCACA GCAGTTAAGT 
GCTGAAGCCG CTGCCGCTGC TCTGCCCACT GAAGGTTTGG CACCAGCAGA CAGCCTTAAT 
GACCCAGCGT CTGAGAGCAA CGGCCATGAG ATGCCAACCG CTGTGACCGG AGCTGTGGTT 
CGCCTCCTTC CACGCACTTC TGCAGAGACT CTAGCCCCGT CAAGTACTTT TGCGCCGTCT 
CAGCCATTGG TGGTTGCCAG CCCTGCCAAG ATGCAAGCGG CCACTGCTTT AGCTGAGGTG 
GCCAACGGGA TCGAATCTGC TGCTGGGAAG CAAGAAGCAC CTCCTGTAGT CGTAAAGCCT 
CCAGTGAAAA AAGAGAACCA GTGGTTTGAT GTTGGTATCG TTAAAGTCAC AAACATGGTC 
GTCACTCACT ACTACGTGCC AGCAGACGAT TCAGCAGTGA CTGATGACGA TTCTGGCGCA 
ATCCCAGACT ACAGCCGAAT GAAGAAAATC GAGTTGTCTC CGGGCACTGC TTACAAGTTC 
CGTGTGGCCG GTATCAACGC ATGCGGCCGA GGGACTTTCT CTGAGGTCTC TGCATTCAAG 
ACCTGCTTAC CGGGATTCCC TGGAGCACCG TGTGCCATCA AAATCAGCAA GAGTCCCGAT 
GGTGCTCACT TGACCTGGGA GCCTCCTTCA GTCACATCGG GGAAAATCAT CGAGTATTCG 
GTGTATCTGG CCATCCAGAG CTCACAGACA GTCGAGGCAA AAGCGTCCGC TCCAGCTCAG 
CTGGCCTTTA TGCGGGTGTA CTGCGGACCC AACCCGTCCT GCCTAGTGCA ATCGTCCAGC 
CTCTCCAATG CCCACATCGA CTACACCACC AAACCCGCTA TCATCTTCCG CATCGCCGCC 
CGCAATGAGA AGGGTTACGG CCCTGCCACG CAAGTCAGGT GGCTACAAGA AACTAGTAAA 
GATGGCTCTG CGGCCAAACC TGCAGCAAAG AGGCCGGTGT CATCACCTGA TATGAAAGGC 
GCTGGTCCAA AGAAGGCCAG GTCTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcfc1b ( XM_005166699.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166756.1 Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.

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CloneID	ODa13858
Clone ID Related Accession (Same CDS sequence)	XM_005166699.4 , XM_005166699.3
Accession Version	XM_005166699.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	host cell factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGCGTG 
ACTAGCAGTG CTGGCATCAA GTCCCCCATC ACAATTATCA CCACTAAGGT TGTTACTCCT 
GGCACAGGGA CTCCAGGGAA AATCATCACA GCTGTGCCTA AGCTGACAGG TGCAGGACAA 
CAGGGAGTCA CACAGGTGGT CCTGAAGGGA GCGCCAGGGC AGCCCGGCAC CATCCTGCGC 
ACTGTGCCTA TGGGTGGTGT GCGGCTGGTT TCACCAGGCA CTGTGTCTGC CGACAAACCA 
ACTGTTACCA CACTGGTCGT CAAGGGCACC ACAGGAGTCA CGACTCTGGG TACAGTAACT 
GGAACTGTAT CAACTAGCGT TGCAGGAGCC AATGTTGCTA ATGCCAGCCT GGCGACTCCT 
GTCACCACAC TGGCCTCCAT CGCCACTTTA TCCAGCCAGG TGATCAATCC CACAGCCATC 
ACTGTATCTG CTGCCCAGAC CAACCTCGCC ACCGCTACAA TACAATCTGC ATCTCAGCCT 
ACTCAAGTAA CCCTGATCAC GACCCCGAGT GGTGCTGAAG CCCAGCCTGG GCAGGATCTC 
CCCGTCTCTT TCCTGGCCTC TCCCACATCA GAACAGCCCT CCACCACCGG TGCAGATGCT 
GGCGATAGCG CGGGTGATGG TGCCACCACC GTCACACTAG TATGCTCTAA CCCGCCCTGC 
GAGACCCACG AGACTGGAAC AACAAACACG GCTACTACAG CTTCTGCCAA AATAGGCACT 
GAACAGATTT GTTCCAACCC TCCCTGTGAA ACGCACGTAA CGGGCACCAC CAACACCTCA 
ACCACCGCCT CTGCAAACAT GGGCAGGGCA CAGCAGGTCT GCACCAACCC GCCATCAGAA 
ACCCATGATA CGGGCACCAC CAACACAGCC ACCACTGCCA GCTGTAATAT GGGCTCCACA 
GAAATGGGTA CCGTGAACAG CAAACCAGGC TCTTCATCGC CGCCTGCGTC CTCCTCTTCT 
GAACCATCGA CACCTGTCTC AGAGAGTGGA GCCGAAGCAG GCGTCATGCG TCCAGAAAAC 
CTCCGCACAG GCACCACATA CACCTCCACC ACCGCCCGCT CCAACATGGG CTCTGACCAG 
ACGGGAACAG TCCAGAGCTC CAATAAGAGC CAAGCCGCCA TCTCCTCTAC AATGATGCCA 
GTGAGCTCCA GCGAATCTCC CGGTGACACA GACACTTCTG ACGTCTCCGG CAGCGTCTGC 
TCAAATCCAC CTTGCGAGAC GCGTGAAACG GGCACCACTA GTACACCTAC ACAATCAAGC 
GCAGATATGG AGAGTGGACA GAGTGGTTCT GTGCAAAGGG TGTGTTCCAA TCCTCCTTGT 
GAAACACATG AGACTGGCAC CACCAACACG TCAACACAGG CATCGTCTTC AATGGCCGCT 
GGACAGAGTG GCACTGCGCA AAGGGTGTGT TCTAACCCTC CTTGTGAAAC ACATGAGACT 
GGCACCACCA ACACGCCGAC ACAGGCCTCA TCTTCATTAG GTGCTGGACA GAGTGGCACT 
GTGCAAAGGG TGTGTTCTAA CCCTCCTTGT GAAACACATG AGACTGGCAC CACCAACACG 
CCGACACAGG CCTCATCTTC ATTAGGTGCT GGACAGAGTG GCACTGTGCA AAGGGTGTGT 
TCTAACCCTC CTTGTGAAAC ACATGAGACT GGCACCACCA ACACGCAGAC CCAGGCTTCT 
TCTACTTTGG GTGCTGGACA GAGTGGTGCT GTGCAAAGGG TGTGTTCCAA CCCTCGTGAA 
ACGCATGAAA CGGGCACCAC CAACACGCCG ACACAGGCCT CATCTGGTAT TGGCGCTGGA 
CAGAATGGCA CCGTGCAAAT GGTCTGTTCC AATCCTCCCT GTGAAACCCA CGAGACGGGC 
ACTACCAACA CAGCCACCAC TGCTACAAGC AACTTAGAAA CTGGAGAAGG AGCAGCTCAG 
CAGGCTGGGG ATGGACAGAG TGAAAGCGCC ACCAGCACAG AGCCCACATC ATCTACAGAA 
CCAGCCAATC CCGCGACACA AAGCAGAGCC ATCACCACAG TAACACAGGC AACGCCCACT 
CCAGGACCAT CAGTACCGGA GATTTCCTCC ATGGCTGGAT CTGCAGTAGT ATCTGAAACT 
CCAGGTGAGG CTGAGGCGAT GGAGCAGGCC AGTGCAGAAG CCGTGGCGGC GGGTGAAGAG 
CCTATGCAGA CGGAGAGTCA GGGAGAGCTG AGTGAAGAGG GAGCCGTCAG GGTCGTCGCC 
ATTGATGAAA GTGCAGCAGG TGACGGGAGC ACTGTGCTTC AGGTTCACGT CGCCATGGAG 
GCACAGCCGG GGCAAGGAGA TCAGGCTGAG CAGATGGAGG CCGGTGTGGA GGCAGCAGAA 
GCCATGAGTC TCGCTGCTCA GGACTCAGAA GCCCTCGCCC TGCCACAGGA GCTGATGTCT 
GCAGAAGGAC AGCAAACCAC CCTGATGGTG ACTGGACTGA CCCCTGAGGA GCTGGCAGTG 
ACGGCGGCGG CTGAGGCAGC TGCACAGGCT GCAGCCACAG AAGAAGCCCA GGCCCTCGCT 
ATCCAGGCCG TCCTGCAGGC GGCTCAGCAG GCGGTGCTCA GTGGGGAAGG AGACGCAGGT 
CACGACGGCC AGCAGGCCAC CACCATCCCT ATCGTTTTGA CTCAGCAGGA ACTTGCAGCT 
CTGGTCCAGC AGCAGCAGCA GCTTCAGGAG GCGCAGGCGG CCGCAGCACA GCAGTTAAGT 
GCTGAAGCCG CTGCCGCTGC TCTGCCCACT GAAGGTTTGG CACCAGCAGA CAGCCTTAAT 
GACCCAGCGT CTGAGAGCAA CGGCCATGAG ATGCCAACCG CTGTGACCGG AGCTGTGGTT 
CGCCTCCTTC CACGCACTTC TGCAGAGACT CTAGCCCCGT CAAGTACTTT TGCGCCGTCT 
CAGCCATTGG TGGTTGCCAG CCCTGCCAAG ATGCAAGCGG CCACTGCTTT AGCTGAGGTG 
GCCAACGGGA TCGAATCTGC TGCTGGGAAG CAAGAAGCAC CTCCTGTAGT CGTAAAGCCT 
CCAGTGAAAA AAGAGAACCA GTGGTTTGAT GTTGGTATCG TTAAAGTCAC AAACATGGTC 
GTCACTCACT ACTACGTGCC AGCAGACGAT TCAGCAGTGA CTGATGACGA TTCTGGCGCA 
ATCCCAGACT ACAGCCGAAT GAAGAAAATC GAGTTGTCTC CGGGCACTGC TTACAAGTTC 
CGTGTGGCCG GTATCAACGC ATGCGGCCGA GGGACTTTCT CTGAGGTCTC TGCATTCAAG 
ACCTGCTTAC CGGGATTCCC TGGAGCACCG TGTGCCATCA AAATCAGCAA GAGTCCCGAT 
GGTGCTCACT TGACCTGGGA GCCTCCTTCA GTCACATCGG GGAAAATCAT CGAGTATTCG 
GTGTATCTGG CCATCCAGAG CTCACAGACA GTCGAGGCAA AAGCGTCCGC TCCAGCTCAG 
CTGGCCTTTA TGCGGGTGTA CTGCGGACCC AACCCGTCCT GCCTAGTGCA ATCGTCCAGC 
CTCTCCAATG CCCACATCGA CTACACCACC AAACCCGCTA TCATCTTCCG CATCGCCGCC 
CGCAATGAGA AGGGTTACGG CCCTGCCACG CAAGTCAGGT GGCTACAAGA AACTAGTAAA 
GATGGCTCTG CGGCCAAACC TGCAGCAAAG AGGCCGGTGT CATCACCTGA TATGAAAGGC 
GCTGGTCCAA AGAAGGCCAG GTCTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166756.1
CDS	739..6711
Translation	MASSGTSVLQPRWKRVLGWSGPVPRPRHGHRAVAIKELMVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKPKAPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYTLELRPGSNVAGWDIPITYGVLPPPRESHTAVVYTEKTSKKSRLIIYGGMSGCRLGDLWTLDIDTLTWNKPAISGAAPLPRSLHSATTITNKMFVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDSMSWETILMDTLEDNIPRARAGHCSVAINNRLYVWSGRDGYRKAWNNQVCCKDLWYLETDRPQPPSRVQLVRANTNSLEVSWGAVPTADTYLLQLQKYDIPAATAATSPTVNPMPSVPVNSPKSPVPAAAAPAAQSMPLSGVTLVPQVPSPTTAMPTSPLAASPRGPAILKVAAPHSTPGTSVVTVRQATPGAKSPVTVTSLPAGVRMVVPAQSTQGTPIGSSPQMSGMAALAAAAAATQKIPPSSATTVLNVPAGATLVKTVAVTPGSTTLPATVKVASPVMVTNPATRMLKTAAAQVGTSAISTPNTPTRPIITVHKSGTVTVAQQAQVVTTMVGGVTKTITLVKSPITMGGSGALYSQLSSLGKVMSVVQTKPVQTSAVTGQAGSSPVTLIQTKTPLPAGTILKLVTSADGKPTTIITTSQAGGTGTKPTILGISSVSPTTTNKPGTTIIKTIPMSALQGVTSSAGIKSPITIITTKVVTPGTGTPGKIITAVPKLTGAGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVSPGTVSADKPTVTTLVVKGTTGVTTLGTVTGTVSTSVAGANVANASLATPVTTLASIATLSSQVINPTAITVSAAQTNLATATIQSASQPTQVTLITTPSGAEAQPGQDLPVSFLASPTSEQPSTTGADAGDSAGDGATTVTLVCSNPPCETHETGTTNTATTASAKIGTEQICSNPPCETHVTGTTNTSTTASANMGRAQQVCTNPPSETHDTGTTNTATTASCNMGSTEMGTVNSKPGSSSPPASSSSEPSTPVSESGAEAGVMRPENLRTGTTYTSTTARSNMGSDQTGTVQSSNKSQAAISSTMMPVSSSESPGDTDTSDVSGSVCSNPPCETRETGTTSTPTQSSADMESGQSGSVQRVCSNPPCETHETGTTNTSTQASSSMAAGQSGTAQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTQTQASSTLGAGQSGAVQRVCSNPRETHETGTTNTPTQASSGIGAGQNGTVQMVCSNPPCETHETGTTNTATTATSNLETGEGAAQQAGDGQSESATSTEPTSSTEPANPATQSRAITTVTQATPTPGPSVPEISSMAGSAVVSETPGEAEAMEQASAEAVAAGEEPMQTESQGELSEEGAVRVVAIDESAAGDGSTVLQVHVAMEAQPGQGDQAEQMEAGVEAAEAMSLAAQDSEALALPQELMSAEGQQTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVLSGEGDAGHDGQQATTIPIVLTQQELAALVQQQQQLQEAQAAAAQQLSAEAAAAALPTEGLAPADSLNDPASESNGHEMPTAVTGAVVRLLPRTSAETLAPSSTFAPSQPLVVASPAKMQAATALAEVANGIESAAGKQEAPPVVVKPPVKKENQWFDVGIVKVTNMVVTHYYVPADDSAVTDDDSGAIPDYSRMKKIELSPGTAYKFRVAGINACGRGTFSEVSAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQTVEAKASAPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDGSAAKPAAKRPVSSPDMKGAGPKKARSDQ

Target ORF information:

RefSeq Version	XM_005166699.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio host cell factor C1b (hcfc1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166699.3

ORF Insert Sequence:

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGCGTG 
ACTAGCAGTG CTGGCATCAA GTCCCCCATC ACAATTATCA CCACTAAGGT TGTTACTCCT 
GGCACAGGGA CTCCAGGGAA AATCATCACA GCTGTGCCTA AGCTGACAGG TGCAGGACAA 
CAGGGAGTCA CACAGGTGGT CCTGAAGGGA GCGCCAGGGC AGCCCGGCAC CATCCTGCGC 
ACTGTGCCTA TGGGTGGTGT GCGGCTGGTT TCACCAGGCA CTGTGTCTGC CGACAAACCA 
ACTGTTACCA CACTGGTCGT CAAGGGCACC ACAGGAGTCA CGACTCTGGG TACAGTAACT 
GGAACTGTAT CAACTAGCGT TGCAGGAGCC AATGTTGCTA ATGCCAGCCT GGCGACTCCT 
GTCACCACAC TGGCCTCCAT CGCCACTTTA TCCAGCCAGG TGATCAATCC CACAGCCATC 
ACTGTATCTG CTGCCCAGAC CAACCTCGCC ACCGCTACAA TACAATCTGC ATCTCAGCCT 
ACTCAAGTAA CCCTGATCAC GACCCCGAGT GGTGCTGAAG CCCAGCCTGG GCAGGATCTC 
CCCGTCTCTT TCCTGGCCTC TCCCACATCA GAACAGCCCT CCACCACCGG TGCAGATGCT 
GGCGATAGCG CGGGTGATGG TGCCACCACC GTCACACTAG TATGCTCTAA CCCGCCCTGC 
GAGACCCACG AGACTGGAAC AACAAACACG GCTACTACAG CTTCTGCCAA AATAGGCACT 
GAACAGATTT GTTCCAACCC TCCCTGTGAA ACGCACGTAA CGGGCACCAC CAACACCTCA 
ACCACCGCCT CTGCAAACAT GGGCAGGGCA CAGCAGGTCT GCACCAACCC GCCATCAGAA 
ACCCATGATA CGGGCACCAC CAACACAGCC ACCACTGCCA GCTGTAATAT GGGCTCCACA 
GAAATGGGTA CCGTGAACAG CAAACCAGGC TCTTCATCGC CGCCTGCGTC CTCCTCTTCT 
GAACCATCGA CACCTGTCTC AGAGAGTGGA GCCGAAGCAG GCGTCATGCG TCCAGAAAAC 
CTCCGCACAG GCACCACATA CACCTCCACC ACCGCCCGCT CCAACATGGG CTCTGACCAG 
ACGGGAACAG TCCAGAGCTC CAATAAGAGC CAAGCCGCCA TCTCCTCTAC AATGATGCCA 
GTGAGCTCCA GCGAATCTCC CGGTGACACA GACACTTCTG ACGTCTCCGG CAGCGTCTGC 
TCAAATCCAC CTTGCGAGAC GCGTGAAACG GGCACCACTA GTACACCTAC ACAATCAAGC 
GCAGATATGG AGAGTGGACA GAGTGGTTCT GTGCAAAGGG TGTGTTCCAA TCCTCCTTGT 
GAAACACATG AGACTGGCAC CACCAACACG TCAACACAGG CATCGTCTTC AATGGCCGCT 
GGACAGAGTG GCACTGCGCA AAGGGTGTGT TCTAACCCTC CTTGTGAAAC ACATGAGACT 
GGCACCACCA ACACGCCGAC ACAGGCCTCA TCTTCATTAG GTGCTGGACA GAGTGGCACT 
GTGCAAAGGG TGTGTTCTAA CCCTCCTTGT GAAACACATG AGACTGGCAC CACCAACACG 
CCGACACAGG CCTCATCTTC ATTAGGTGCT GGACAGAGTG GCACTGTGCA AAGGGTGTGT 
TCTAACCCTC CTTGTGAAAC ACATGAGACT GGCACCACCA ACACGCAGAC CCAGGCTTCT 
TCTACTTTGG GTGCTGGACA GAGTGGTGCT GTGCAAAGGG TGTGTTCCAA CCCTCGTGAA 
ACGCATGAAA CGGGCACCAC CAACACGCCG ACACAGGCCT CATCTGGTAT TGGCGCTGGA 
CAGAATGGCA CCGTGCAAAT GGTCTGTTCC AATCCTCCCT GTGAAACCCA CGAGACGGGC 
ACTACCAACA CAGCCACCAC TGCTACAAGC AACTTAGAAA CTGGAGAAGG AGCAGCTCAG 
CAGGCTGGGG ATGGACAGAG TGAAAGCGCC ACCAGCACAG AGCCCACATC ATCTACAGAA 
CCAGCCAATC CCGCGACACA AAGCAGAGCC ATCACCACAG TAACACAGGC AACGCCCACT 
CCAGGACCAT CAGTACCGGA GATTTCCTCC ATGGCTGGAT CTGCAGTAGT ATCTGAAACT 
CCAGGTGAGG CTGAGGCGAT GGAGCAGGCC AGTGCAGAAG CCGTGGCGGC GGGTGAAGAG 
CCTATGCAGA CGGAGAGTCA GGGAGAGCTG AGTGAAGAGG GAGCCGTCAG GGTCGTCGCC 
ATTGATGAAA GTGCAGCAGG TGACGGGAGC ACTGTGCTTC AGGTTCACGT CGCCATGGAG 
GCACAGCCGG GGCAAGGAGA TCAGGCTGAG CAGATGGAGG CCGGTGTGGA GGCAGCAGAA 
GCCATGAGTC TCGCTGCTCA GGACTCAGAA GCCCTCGCCC TGCCACAGGA GCTGATGTCT 
GCAGAAGGAC AGCAAACCAC CCTGATGGTG ACTGGACTGA CCCCTGAGGA GCTGGCAGTG 
ACGGCGGCGG CTGAGGCAGC TGCACAGGCT GCAGCCACAG AAGAAGCCCA GGCCCTCGCT 
ATCCAGGCCG TCCTGCAGGC GGCTCAGCAG GCGGTGCTCA GTGGGGAAGG AGACGCAGGT 
CACGACGGCC AGCAGGCCAC CACCATCCCT ATCGTTTTGA CTCAGCAGGA ACTTGCAGCT 
CTGGTCCAGC AGCAGCAGCA GCTTCAGGAG GCGCAGGCGG CCGCAGCACA GCAGTTAAGT 
GCTGAAGCCG CTGCCGCTGC TCTGCCCACT GAAGGTTTGG CACCAGCAGA CAGCCTTAAT 
GACCCAGCGT CTGAGAGCAA CGGCCATGAG ATGCCAACCG CTGTGACCGG AGCTGTGGTT 
CGCCTCCTTC CACGCACTTC TGCAGAGACT CTAGCCCCGT CAAGTACTTT TGCGCCGTCT 
CAGCCATTGG TGGTTGCCAG CCCTGCCAAG ATGCAAGCGG CCACTGCTTT AGCTGAGGTG 
GCCAACGGGA TCGAATCTGC TGCTGGGAAG CAAGAAGCAC CTCCTGTAGT CGTAAAGCCT 
CCAGTGAAAA AAGAGAACCA GTGGTTTGAT GTTGGTATCG TTAAAGTCAC AAACATGGTC 
GTCACTCACT ACTACGTGCC AGCAGACGAT TCAGCAGTGA CTGATGACGA TTCTGGCGCA 
ATCCCAGACT ACAGCCGAAT GAAGAAAATC GAGTTGTCTC CGGGCACTGC TTACAAGTTC 
CGTGTGGCCG GTATCAACGC ATGCGGCCGA GGGACTTTCT CTGAGGTCTC TGCATTCAAG 
ACCTGCTTAC CGGGATTCCC TGGAGCACCG TGTGCCATCA AAATCAGCAA GAGTCCCGAT 
GGTGCTCACT TGACCTGGGA GCCTCCTTCA GTCACATCGG GGAAAATCAT CGAGTATTCG 
GTGTATCTGG CCATCCAGAG CTCACAGACA GTCGAGGCAA AAGCGTCCGC TCCAGCTCAG 
CTGGCCTTTA TGCGGGTGTA CTGCGGACCC AACCCGTCCT GCCTAGTGCA ATCGTCCAGC 
CTCTCCAATG CCCACATCGA CTACACCACC AAACCCGCTA TCATCTTCCG CATCGCCGCC 
CGCAATGAGA AGGGTTACGG CCCTGCCACG CAAGTCAGGT GGCTACAAGA AACTAGTAAA 
GATGGCTCTG CGGCCAAACC TGCAGCAAAG AGGCCGGTGT CATCACCTGA TATGAAAGGC 
GCTGGTCCAA AGAAGGCCAG GTCTGACCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hcfc1b ( XM_021478283.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333958.1 Danio rerio host cell factor C1b (hcfc1b), transcript variant X3, mRNA.

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CloneID	ODa143704
Clone ID Related Accession (Same CDS sequence)	XM_021478283.1
Accession Version	XM_021478283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	host cell factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC AGACCCAGGC TTCTTCTACT TTGGGTGCTG GACAGAGTGG TGCTGTGCAA 
AGGGTGTGTT CCAACCCTCG TGAAACGCAT GAAACGGGCA CCACCAACAC GCCGACACAG 
GCCTCATCTG GTATTGGCGC TGGACAGAAT GGCACCGTGC AAATGGTCTG TTCCAATCCT 
CCCTGTGAAA CCCACGAGAC GGGCACTACC AACACAGCCA CCACTGCTAC AAGCAACTTA 
GAAACTGGAG AAGGAGCAGC TCAGCAGGCT GGGGATGGAC AGAGTGAAAG CGCCACCAGC 
ACAGAGCCCA CATCATCTAC AGAACCAGCC AATCCCGCGA CACAAAGCAG AGCCATCACC 
ACAGTAACAC AGGCAACGCC CACTCCAGGA CCATCAGTAC CGGAGATTTC CTCCATGGCT 
GGATCTGCAG TAGTATCTGA AACTCCAGGT GAGGCTGAGG CGATGGAGCA GGCCAGTGCA 
GAAGCCGTGG CGGCGGGTGA AGAGCCTATG CAGACGGAGA GTCAGGGAGA GCTGAGTGAA 
GAGGGAGCCG TCAGGGTCGT CGCCATTGAT GAAAGTGCAG CAGGTGACGG GAGCACTGTG 
CTTCAGGTTC ACGTCGCCAT GGAGGCACAG CCGGGGCAAG GAGATCAGGC TGAGCAGATG 
GAGGCCGGTG TGGAGGCAGC AGAAGCCATG AGTCTCGCTG CTCAGGACTC AGAAGCCCTC 
GCCCTGCCAC AGGAGCTGAT GTCTGCAGAA GGACAGCAAA CCACCCTGAT GGTGACTGGA 
CTGACCCCTG AGGAGCTGGC AGTGACGGCG GCGGCTGAGG CAGCTGCACA GGCTGCAGCC 
ACAGAAGAAG CCCAGGCCCT CGCTATCCAG GCCGTCCTGC AGGCGGCTCA GCAGGCGGTG 
CTCAGTGGGG AAGGAGACGC AGGTCACGAC GGCCAGCAGG CCACCACCAT CCCTATCGTT 
TTGACTCAGC AGGAACTTGC AGCTCTGGTC CAGCAGCAGC AGCAGCTTCA GGAGGCGCAG 
GCGGCCGCAG CACAGCAGTT AAGTGCTGAA GCCGCTGCCG CTGCTCTGCC CACTGAAGGT 
TTGGCACCAG CAGACAGCCT TAATGACCCA GCGTCTGAGA GCAACGGCCA TGAGATGCCA 
ACCGCTGTGA CCGGAGCTGT GGTTCGCCTC CTTCCACGCA CTTCTGCAGA GACTCTAGCC 
CCGTCAAGTA CTTTTGCGCC GTCTCAGCCA TTGGTGGTTG CCAGCCCTGC CAAGATGCAA 
GCGGCCACTG CTTTAGCTGA GGTGGCCAAC GGGATCGAAT CTGCTGCTGG GAAGCAAGAA 
GCACCTCCTG TAGTCGTAAA GCCTCCAGTG AAAAAAGAGA ACCAGTGGTT TGATGTTGGT 
ATCGTTAAAG TCACAAACAT GGTCGTCACT CACTACTACG TGCCAGCAGA CGATTCAGCA 
GTGACTGATG ACGATTCTGG CGCAATCCCA GACTACAGCC GAATGAAGAA AATCGAGTTG 
TCTCCGGGCA CTGCTTACAA GTTCCGTGTG GCCGGTATCA ACGCATGCGG CCGAGGGACT 
TTCTCTGAGG TCTCTGCATT CAAGACCTGC TTACCGGGAT TCCCTGGAGC ACCGTGTGCC 
ATCAAAATCA GCAAGAGTCC CGATGGTGCT CACTTGACCT GGGAGCCTCC TTCAGTCACA 
TCGGGGAAAA TCATCGAGTA TTCGGTGTAT CTGGCCATCC AGAGCTCACA GACAGTCGAG 
GCAAAAGCGT CCGCTCCAGC TCAGCTGGCC TTTATGCGGG TGTACTGCGG ACCCAACCCG 
TCCTGCCTAG TGCAATCGTC CAGCCTCTCC AATGCCCACA TCGACTACAC CACCAAACCC 
GCTATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGTT ACGGCCCTGC CACGCAAGTC 
AGGTGGCTAC AAGAAACTAG TAAAGATGGC TCTGCGGCCA AACCTGCAGC AAAGAGGCCG 
GTGTCATCAC CTGATATGAA AGGCGCTGGT CCAAAGAAGG CCAGGTCTGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333958.1
CDS	191..6067
Translation	MASSGTSVLQPRWKRVLGWSGPVPRPRHGHRAVAIKELMVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKPKAPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYTLELRPGSNVAGWDIPITYGVLPPPRESHTAVVYTEKTSKKSRLIIYGGMSGCRLGDLWTLDIDTLTWNKPAISGAAPLPRSLHSATTITNKMFVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDSMSWETILMDTLEDNIPRARAGHCSVAINNRLYVWSGRDGYRKAWNNQVCCKDLWYLETDRPQPPSRVQLVRANTNSLEVSWGAVPTADTYLLQLQKYDIPAATAATSPTVNPMPSVPVNSPKSPVPAAAAPAAQSMPLSGVTLVPQVPSPTTAMPTSPLAASPRGPAILKVAAPHSTPGTSVVTVRQATPGAKSPVTVTSLPAGVRMVVPAQSTQGTPIGSSPQMSGMAALAAAAAATQKIPPSSATTVLNVPAGATLVKTVAVTPGSTTLPATVKVASPVMVTNPATRMLKTAAAQVGTSAISTPNTPTRPIITVHKSGTVTVAQQAQVVTTMVGGVTKTITLVKSPITMGGSGALYSQLSSLGKVMSVVQTKPVQTSAVTGQAGSSPVTLIQTKTPLPAGTILKLVTSADGKPTTIITTSQAGGTGTKPTILGISSVSPTTTNKPGTTIIKTIPMSALQGATGVTSSAGIKSPITIITTKVVTPGTGTPGKIITAVPKLTGAGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVSPGTVSADKPTVTTLVVKGTTGVTTLGTVTGTVSTSVAGANVANASLATPVTTLASIATLSSQVINPTAITVSAAQTNLATATIQSASQPTQVTLITTPSGAEAQPGQDLPVSFLASPTSEQPSTTGADAGDSAGDGATTVTLVCSNPPCETHETGTTNTATTASAKIGTEQICSNPPCETHVTGTTNTSTTASANMGRAQQVCTNPPSETHDTGTTNTATTASCNMGSTEMGTVNSKPGSSSPPASSSSEPSTPVSESGAEAGVMRPENLRTGTTYTSTTARSNMGSDQTGTVQSSNKSQAAISSTMMPVSSSESPGDTDTSDVSGSVCSNPPCETRETGTTSTPTQSSADMESGQSGSVQRVCSNPPCETHETGTTNTSTQASSSMAAGQSGTAQRVCSNPPCETHETGTTNTPTQASSSLGAGQSGTVQRVCSNPPCETHETGTTNTQTQASSTLGAGQSGAVQRVCSNPRETHETGTTNTPTQASSGIGAGQNGTVQMVCSNPPCETHETGTTNTATTATSNLETGEGAAQQAGDGQSESATSTEPTSSTEPANPATQSRAITTVTQATPTPGPSVPEISSMAGSAVVSETPGEAEAMEQASAEAVAAGEEPMQTESQGELSEEGAVRVVAIDESAAGDGSTVLQVHVAMEAQPGQGDQAEQMEAGVEAAEAMSLAAQDSEALALPQELMSAEGQQTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVLSGEGDAGHDGQQATTIPIVLTQQELAALVQQQQQLQEAQAAAAQQLSAEAAAAALPTEGLAPADSLNDPASESNGHEMPTAVTGAVVRLLPRTSAETLAPSSTFAPSQPLVVASPAKMQAATALAEVANGIESAAGKQEAPPVVVKPPVKKENQWFDVGIVKVTNMVVTHYYVPADDSAVTDDDSGAIPDYSRMKKIELSPGTAYKFRVAGINACGRGTFSEVSAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQTVEAKASAPAQLAFMRVYCGPNPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDGSAAKPAAKRPVSSPDMKGAGPKKARSDQ

Target ORF information:

RefSeq Version	XM_021478283.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio host cell factor C1b (hcfc1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478283.1

ORF Insert Sequence:

ATGGCTTCTT CAGGCACTTC GGTTCTGCAG CCGCGCTGGA AGAGGGTTTT GGGCTGGTCT 
GGCCCGGTTC CTCGCCCGAG ACATGGACAC CGAGCTGTGG CCATTAAAGA GCTGATGGTG 
GTGTTCGGCG GAGGAAACGA AGGCATTGTG GATGAGCTGC ACGTTTACAA CACAGCCACC 
AATCAGTGGT TTATTCCGGC TGTCCGGGGT GACATTCCTC CCGGCTGTGC TGCGTATGGA 
TTTGTGTGTG ATGGCACCAG ACTCCTTGTG TTCGGGGGCA TGGTTGAGTA CGGGAAGTAC 
AGCAACGATC TGTATGAACT ACAAGCTAGT AGATGGGAGT GGAAACGATT GAAGCCCAAA 
GCCCCTAAGA ATGGCCCACC ACCATGCCCT CGTCTGGGCC ACAGCTTTTC TCTAGTTGGA 
AACAAGTGTT ACTTGTTTGG TGGATTGGCA AATGACAGCG AAGATCCCAA AAACAATATT 
CCAAGATACC TGAATGACCT CTACACACTG GAGTTGCGCC CGGGGTCTAA TGTAGCAGGC 
TGGGACATCC CGATTACATA CGGCGTGCTT CCTCCTCCCC GTGAGAGCCA CACAGCTGTC 
GTCTACACAG AGAAAACCTC CAAAAAGTCT CGTCTCATCA TCTACGGCGG CATGAGTGGC 
TGCCGTCTTG GAGACCTCTG GACTTTAGAC ATCGATACTT TAACCTGGAA CAAGCCTGCC 
ATAAGTGGTG CAGCACCCTT ACCCCGCAGT CTCCACTCGG CCACCACTAT AACCAACAAG 
ATGTTTGTGT TTGGTGGATG GGTCCCTCTG GTTATGGACG ACGTCAAAGT AGCAACACAC 
GAGAAGGAAT GGAAGTGCAC AAACACGTTG GCCTGCTTAA ATCTGGACTC GATGTCCTGG 
GAAACAATCT TGATGGACAC TCTCGAGGAT AATATCCCAA GGGCTAGAGC CGGACACTGT 
TCTGTGGCGA TAAACAACAG GCTGTATGTC TGGAGTGGAC GAGATGGTTA CCGCAAAGCC 
TGGAACAACC AAGTTTGCTG CAAAGATCTG TGGTATTTGG AGACCGATCG TCCTCAGCCT 
CCGTCCCGTG TGCAGCTGGT TCGTGCCAAC ACCAATTCTC TGGAGGTGAG CTGGGGTGCC 
GTTCCTACGG CAGACACGTA CCTGTTACAG CTGCAAAAAT ACGACATCCC TGCTGCGACA 
GCTGCCACGT CACCAACGGT TAACCCTATG CCGTCTGTAC CCGTAAACTC ACCCAAAAGT 
CCGGTGCCTG CCGCCGCTGC TCCTGCAGCT CAGAGTATGC CACTCTCTGG GGTCACACTG 
GTGCCACAAG TCCCTTCCCC AACCACCGCC ATGCCAACCA GCCCGCTCGC CGCCTCTCCT 
CGTGGACCAG CTATTCTTAA GGTGGCAGCG CCTCATTCTA CCCCTGGAAC TTCAGTCGTT 
ACTGTGCGTC AGGCCACACC AGGAGCAAAA TCCCCAGTGA CGGTTACATC ACTTCCTGCA 
GGTGTTCGTA TGGTTGTTCC TGCGCAGAGC ACACAGGGCA CGCCAATCGG CAGCAGTCCT 
CAGATGAGCG GCATGGCTGC ATTAGCTGCA GCTGCAGCCG CTACACAGAA GATCCCACCT 
TCCTCCGCGA CTACTGTGCT AAATGTTCCA GCCGGAGCCA CTCTTGTGAA GACCGTCGCT 
GTGACCCCTG GATCCACGAC TCTGCCAGCT ACTGTCAAAG TAGCTTCACC TGTTATGGTT 
ACTAATCCTG CGACTCGGAT GCTAAAAACA GCAGCAGCGC AGGTCGGCAC TTCCGCCATC 
TCGACCCCCA ACACCCCGAC TCGCCCCATC ATCACTGTGC ACAAATCCGG CACAGTAACC 
GTAGCCCAGC AGGCCCAAGT TGTTACCACC ATGGTTGGTG GAGTCACCAA AACTATAACA 
CTTGTCAAGA GCCCAATAAC AATGGGTGGC AGTGGTGCTC TGTATTCTCA GCTCTCCAGC 
CTTGGGAAGG TGATGTCTGT TGTCCAGACT AAACCAGTGC AAACATCTGC AGTCACCGGA 
CAGGCCGGAA GCAGTCCTGT CACTTTGATT CAGACTAAGA CGCCTCTTCC TGCTGGTACC 
ATCCTGAAAC TGGTCACATC TGCTGATGGC AAGCCAACCA CAATCATTAC AACTTCACAG 
GCTGGAGGGA CTGGCACCAA ACCCACCATC CTGGGCATCA GCAGTGTCTC TCCAACTACC 
ACCAACAAAC CAGGAACCAC CATCATTAAG ACCATTCCCA TGTCTGCATT ACAAGGTGCA 
ACAGGCGTGA CTAGCAGTGC TGGCATCAAG TCCCCCATCA CAATTATCAC CACTAAGGTT 
GTTACTCCTG GCACAGGGAC TCCAGGGAAA ATCATCACAG CTGTGCCTAA GCTGACAGGT 
GCAGGACAAC AGGGAGTCAC ACAGGTGGTC CTGAAGGGAG CGCCAGGGCA GCCCGGCACC 
ATCCTGCGCA CTGTGCCTAT GGGTGGTGTG CGGCTGGTTT CACCAGGCAC TGTGTCTGCC 
GACAAACCAA CTGTTACCAC ACTGGTCGTC AAGGGCACCA CAGGAGTCAC GACTCTGGGT 
ACAGTAACTG GAACTGTATC AACTAGCGTT GCAGGAGCCA ATGTTGCTAA TGCCAGCCTG 
GCGACTCCTG TCACCACACT GGCCTCCATC GCCACTTTAT CCAGCCAGGT GATCAATCCC 
ACAGCCATCA CTGTATCTGC TGCCCAGACC AACCTCGCCA CCGCTACAAT ACAATCTGCA 
TCTCAGCCTA CTCAAGTAAC CCTGATCACG ACCCCGAGTG GTGCTGAAGC CCAGCCTGGG 
CAGGATCTCC CCGTCTCTTT CCTGGCCTCT CCCACATCAG AACAGCCCTC CACCACCGGT 
GCAGATGCTG GCGATAGCGC GGGTGATGGT GCCACCACCG TCACACTAGT ATGCTCTAAC 
CCGCCCTGCG AGACCCACGA GACTGGAACA ACAAACACGG CTACTACAGC TTCTGCCAAA 
ATAGGCACTG AACAGATTTG TTCCAACCCT CCCTGTGAAA CGCACGTAAC GGGCACCACC 
AACACCTCAA CCACCGCCTC TGCAAACATG GGCAGGGCAC AGCAGGTCTG CACCAACCCG 
CCATCAGAAA CCCATGATAC GGGCACCACC AACACAGCCA CCACTGCCAG CTGTAATATG 
GGCTCCACAG AAATGGGTAC CGTGAACAGC AAACCAGGCT CTTCATCGCC GCCTGCGTCC 
TCCTCTTCTG AACCATCGAC ACCTGTCTCA GAGAGTGGAG CCGAAGCAGG CGTCATGCGT 
CCAGAAAACC TCCGCACAGG CACCACATAC ACCTCCACCA CCGCCCGCTC CAACATGGGC 
TCTGACCAGA CGGGAACAGT CCAGAGCTCC AATAAGAGCC AAGCCGCCAT CTCCTCTACA 
ATGATGCCAG TGAGCTCCAG CGAATCTCCC GGTGACACAG ACACTTCTGA CGTCTCCGGC 
AGCGTCTGCT CAAATCCACC TTGCGAGACG CGTGAAACGG GCACCACTAG TACACCTACA 
CAATCAAGCG CAGATATGGA GAGTGGACAG AGTGGTTCTG TGCAAAGGGT GTGTTCCAAT 
CCTCCTTGTG AAACACATGA GACTGGCACC ACCAACACGT CAACACAGGC ATCGTCTTCA 
ATGGCCGCTG GACAGAGTGG CACTGCGCAA AGGGTGTGTT CTAACCCTCC TTGTGAAACA 
CATGAGACTG GCACCACCAA CACGCCGACA CAGGCCTCAT CTTCATTAGG TGCTGGACAG 
AGTGGCACTG TGCAAAGGGT GTGTTCTAAC CCTCCTTGTG AAACACATGA GACTGGCACC 
ACCAACACGC AGACCCAGGC TTCTTCTACT TTGGGTGCTG GACAGAGTGG TGCTGTGCAA 
AGGGTGTGTT CCAACCCTCG TGAAACGCAT GAAACGGGCA CCACCAACAC GCCGACACAG 
GCCTCATCTG GTATTGGCGC TGGACAGAAT GGCACCGTGC AAATGGTCTG TTCCAATCCT 
CCCTGTGAAA CCCACGAGAC GGGCACTACC AACACAGCCA CCACTGCTAC AAGCAACTTA 
GAAACTGGAG AAGGAGCAGC TCAGCAGGCT GGGGATGGAC AGAGTGAAAG CGCCACCAGC 
ACAGAGCCCA CATCATCTAC AGAACCAGCC AATCCCGCGA CACAAAGCAG AGCCATCACC 
ACAGTAACAC AGGCAACGCC CACTCCAGGA CCATCAGTAC CGGAGATTTC CTCCATGGCT 
GGATCTGCAG TAGTATCTGA AACTCCAGGT GAGGCTGAGG CGATGGAGCA GGCCAGTGCA 
GAAGCCGTGG CGGCGGGTGA AGAGCCTATG CAGACGGAGA GTCAGGGAGA GCTGAGTGAA 
GAGGGAGCCG TCAGGGTCGT CGCCATTGAT GAAAGTGCAG CAGGTGACGG GAGCACTGTG 
CTTCAGGTTC ACGTCGCCAT GGAGGCACAG CCGGGGCAAG GAGATCAGGC TGAGCAGATG 
GAGGCCGGTG TGGAGGCAGC AGAAGCCATG AGTCTCGCTG CTCAGGACTC AGAAGCCCTC 
GCCCTGCCAC AGGAGCTGAT GTCTGCAGAA GGACAGCAAA CCACCCTGAT GGTGACTGGA 
CTGACCCCTG AGGAGCTGGC AGTGACGGCG GCGGCTGAGG CAGCTGCACA GGCTGCAGCC 
ACAGAAGAAG CCCAGGCCCT CGCTATCCAG GCCGTCCTGC AGGCGGCTCA GCAGGCGGTG 
CTCAGTGGGG AAGGAGACGC AGGTCACGAC GGCCAGCAGG CCACCACCAT CCCTATCGTT 
TTGACTCAGC AGGAACTTGC AGCTCTGGTC CAGCAGCAGC AGCAGCTTCA GGAGGCGCAG 
GCGGCCGCAG CACAGCAGTT AAGTGCTGAA GCCGCTGCCG CTGCTCTGCC CACTGAAGGT 
TTGGCACCAG CAGACAGCCT TAATGACCCA GCGTCTGAGA GCAACGGCCA TGAGATGCCA 
ACCGCTGTGA CCGGAGCTGT GGTTCGCCTC CTTCCACGCA CTTCTGCAGA GACTCTAGCC 
CCGTCAAGTA CTTTTGCGCC GTCTCAGCCA TTGGTGGTTG CCAGCCCTGC CAAGATGCAA 
GCGGCCACTG CTTTAGCTGA GGTGGCCAAC GGGATCGAAT CTGCTGCTGG GAAGCAAGAA 
GCACCTCCTG TAGTCGTAAA GCCTCCAGTG AAAAAAGAGA ACCAGTGGTT TGATGTTGGT 
ATCGTTAAAG TCACAAACAT GGTCGTCACT CACTACTACG TGCCAGCAGA CGATTCAGCA 
GTGACTGATG ACGATTCTGG CGCAATCCCA GACTACAGCC GAATGAAGAA AATCGAGTTG 
TCTCCGGGCA CTGCTTACAA GTTCCGTGTG GCCGGTATCA ACGCATGCGG CCGAGGGACT 
TTCTCTGAGG TCTCTGCATT CAAGACCTGC TTACCGGGAT TCCCTGGAGC ACCGTGTGCC 
ATCAAAATCA GCAAGAGTCC CGATGGTGCT CACTTGACCT GGGAGCCTCC TTCAGTCACA 
TCGGGGAAAA TCATCGAGTA TTCGGTGTAT CTGGCCATCC AGAGCTCACA GACAGTCGAG 
GCAAAAGCGT CCGCTCCAGC TCAGCTGGCC TTTATGCGGG TGTACTGCGG ACCCAACCCG 
TCCTGCCTAG TGCAATCGTC CAGCCTCTCC AATGCCCACA TCGACTACAC CACCAAACCC 
GCTATCATCT TCCGCATCGC CGCCCGCAAT GAGAAGGGTT ACGGCCCTGC CACGCAAGTC 
AGGTGGCTAC AAGAAACTAG TAAAGATGGC TCTGCGGCCA AACCTGCAGC AAAGAGGCCG 
GTGTCATCAC CTGATATGAA AGGCGCTGGT CCAAAGAAGG CCAGGTCTGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Hcfc1b, a zebrafish ortholog of HCFC1, regulates craniofacial development by modulating mmachc expression.
Developmental biology396(1)94-106(2014 Dec)
Quintana AM,Geiger EA,Achilly N,Rosenblatt DS,Maclean KN,Stabler SP,Artinger KB,Appel B,Shaikh TH

The first mecp2-null zebrafish model shows altered motor behaviors.
Frontiers in neural circuits7118(2013)
Pietri T,Roman AC,Guyon N,Romano SA,Washbourne P,Moens CB,de Polavieja GG,Sumbre G

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hcfc1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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