dlg5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Gene Symbol	dlg5a
Entrez Gene ID	569065
Full Name	discs, large homolog 5a (Drosophila)
Synonyms	dlg5,wu:fc28f03
General protein information	Preferred Names discs, large homolog 5a (Drosophila) Names disks large homolog 5 discs, large homolog 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145603.1	NP_001139075.1	disks large homolog 5
XM_005156681.4	XP_005156738.1	disks large homolog 5 isoform X1
XM_005156682.4	XP_005156739.1	disks large homolog 5 isoform X2
XM_021480646.1	XP_021336321.1	disks large homolog 5 isoform X4
XM_005156681.3	XP_005156738.1	disks large homolog 5 isoform X1
XM_005156682.3	XP_005156739.1	disks large homolog 5 isoform X2
XM_009307256.2	XP_009305531.1	disks large homolog 5 isoform X3
XM_009307256.3	XP_009305531.1	disks large homolog 5 isoform X3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dlg5	XP_003751471.1
Homo sapiens (human)	DLG5	NP_004738.3
Pan troglodytes (chimpanzee)	DLG5	XP_003951739.1
Canis lupus familiaris (dog)	DLG5	XP_546179.3
Bos taurus (cattle)	DLG5	NP_001193017.1
Gallus gallus (chicken)	DLG5	XP_421604.3
Danio rerio (zebrafish)	dlg5a	NP_001139075.1
Mus musculus (house mouse)	Dlg5	NP_001156985.1
Xenopus tropicalis (tropical clawed frog)	dlg5	XP_002937176.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array.
Tsai HY, Hamilton A, Tinch AE, Guy DR, Gharbi K, Stear MJ, Matika O, Bishop SC, Houston RD
BMC genomics16969(2015 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Statistical evidence for a more than 800-million-year-old evolutionarily conserved genomic region in our genome.
Danchin EG, Pontarotti P
Journal of molecular evolution59(5)587-97(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dlg5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlg5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148544	XM_021480646.1 Latest version!	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa04310	NM_001145603.1 Latest version!	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18634	XM_005156681.4 Latest version!	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18635	XM_005156682.4 Latest version!	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45645	XM_009307256.3 Latest version!	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa18634	XM_005156681.3	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa18635	XM_005156682.3	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45645	XM_009307256.2	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlg5a ( XM_021480646.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336321.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148544
Clone ID Related Accession (Same CDS sequence)	XM_021480646.1
Accession Version	XM_021480646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disks large homolog 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGATC TCTTTACCGT 
AACAGATCCT TTCTGAGAAT CCCTCTGGCT GCTAGGCCTA AGTTCTGCTC TCTCAGAAGC 
TTGAGGTTTG TTCCCTGCCA GTTACGCACA GGGAATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336321.1
CDS	988..5229
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRSLYRNRSFLRIPLAARPKFCSLRSLRFVPCQLRTGNA

Target ORF information:

RefSeq Version	XM_021480646.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480646.1

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGATC TCTTTACCGT 
AACAGATCCT TTCTGAGAAT CCCTCTGGCT GCTAGGCCTA AGTTCTGCTC TCTCAGAAGC 
TTGAGGTTTG TTCCCTGCCA GTTACGCACA GGGAATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( NM_001145603.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139075.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), mRNA.

Price & Availability↑

CloneID	ODa04310
Clone ID Related Accession (Same CDS sequence)	NM_001145603.1
Accession Version	NM_001145603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	disks large homolog 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX323467.8, BX323798.6 and AL645926.16. On Mar 5, 2009 this sequence version replaced XM_692428.3.

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGTACCAAAG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAT AACAGCCGTC 
ATGAAGAACC CTGTTTACAT GGTGCGCAGT CACAGAGTTC ACACTAACAA CTGCCCTACT 
GTCGTCAGCC AGATCAATCA GCAGCACACG CACCCCAGTC CTCAGCATCA AGGACAACTG 
AGTCTGGACC TAAGCCAGAA ACGCTCCACG GACTATTCTG AGTCGTCCCG TAGCAGCCGG 
GCCTCACACG GCACCAACTC CCTGCCGTCC AGTGCAAGAC TCGGTTCATC AAACAACTTG 
CAGTATCGCA CTGAAAGGAT TAAGATTCCC TCCACACCGC GATATCCTCG CTCTATTCTG 
GGGTCAGACA GAGGGTCCCT GTCTCATTCA GAATGCAGCA GTCCCAGTCT CATTACTCCC 
CCTCTTTCTC CTCTCAATCT GGAGACGTCT TCGTTTGCCT CCAGCCAGTC TCAGGGCTCC 
ATCTCCACCC TGCCCAGGAT CTCCGTCAGC CCCGTACCCA CAGAAGAGCG CAGAAAAGAC 
AGGCCGTACT TGGAGGAGCC ACGGAATGTT ATGGTGCACA AAGGGGCGGA GCCTCTCGGC 
ATCTCCATTG TGAGTGGAGA GAACGGAGGA ATCTTTGTCT CCAAGGTGAC AGGAGGCAGT 
ATCGCGCACC AGGCTGGGTT GGAGTATGGG GACCAGCTAT TGGAGTATAA TGGAATAAAC 
CTGCGCAATG CCACTGAACA GCAAGCACGA CTCATCATTG GCCAGCAGTG TGACACCATC 
ACGATCATGG CCCAATACAA CCCACACATG TATCAGCTCG GCAACCACTC GCGCTCCAGT 
TCCCGCCTGG AGCCAGTGAG CACTCAATCC ACGCCACAGG GCAGTGGAAC TGCAACCCCA 
GATAACCACT CTACCATAGA CACCCTGAGT GAACAGGATG AAGGCACACT CACCCCATCC 
TCCAAACAGA CCACACCCAC AACCAGTCCC AACAGCTTCA TCAGAATGCC GTCTGAAAGT 
TCCAAGAAAG TACCAGAGGC GCGTTTGGTG ACAGTGAGGA AGACTCAGGT GGAGCTGGGC 
ATTCAGCTGT GTGGAGGAAA CCTGCGGGGA ATCTTCGTGG AGAGGCTGGA AGATGACAGT 
CCTGCCAGAG GAGCGGATGC TGTTGTACCC GGGGACATGA TACTTGAGTA TGGCTCAGTT 
AATATGAAGA ATAAGACTGT GGAGGAGGCA TATCTGGAAA TGCTGAAGCC TGCAGAAACA 
GTGACGTTAA GAGTCCAGCA TCGTCTGGAT GAGTTCAACC AGGTGAAAGG CACTCCTGGA 
GATGGATTCT ATATCAGAGC ACTTTATGAC CGAGTAGCTG AGACTGAGCT CGACCTCTCT 
TTTAAGAAAG ACGACATCCT CTATGTGGAC GACACACTAC CAAATGGCAA CTTCGGCACC 
TGGATGGCCT GGCAACTTGA CGAAAATGCA CAAAAAATTC AAAGAGGCCA GATTCCTAGC 
AAATTTATGA TGGATCAGGA GTTTTACCGT AGACACAGTA TGACTGAACT GAAGGACGAA 
ACGGGCTCCA GTAAAACCCT CTCGGCTGCT GCACGCAGAT CCTTCTTCCG CAGGAAACAC 
AAGCACAAAC GCAGCAGCTC CAAAGACGGG AAAGACGTAG TGGCTTTGGA CGCCATCAGC 
ACAGACTCAA TTCCTTTCTT AGACGACTGT GTGAGTCTGG CTTATCAGAG AGTGCAGAAG 
ATTGACTGCA CATCTCCCAG ACCTGTGCTG ATCCTCGGCC CGCTTGTGGA TCCTGTTAAA 
GACATGCTGG TCAAAGATTC TCCAGGGAAA TTCAGCAGAT GTGTTCTTGA GGTGATGAAG 
GCTTCTCAGC AGGCCATTGA GCGTGGGGTC AAAGACTTTC TCTTCATCGA CTACAAACGA 
AGGAGTGGCC ATTTTGATGT GACGACTGTT GCCTCAATCA ATGAAATCAC GGACAAGGAC 
TGTCACTGTT TGCTTGACAT TGCACCTCAT GCTATTGAAA GGCTTCACAG CGTTCACATT 
TACCCAATCG TCATTTTCAT ACGCTACAAA AACGCAAAGC AGATAAAAGA GCAGAAGGAC 
CCGATTTATC TGCGGGATAA AGTCTCTCAA AAACATTCCA AGGAGCAGTT TGAGATTGCA 
CAGAAGATTG AGCAAGACTA CAGCAAGTTC TTCACAGGTA TCGTTCAGGG AGGCACCTTC 
CCTTACATTT GCACTCAGAT CGTGACTATT GTGGATCAGG AACAGAGCAA AGTCTTGTGG 
ACTCCACTGG GCTCTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139075.1
CDS	1..5781
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPITAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	NM_001145603.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145603.1

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGTACCAAAG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAT AACAGCCGTC 
ATGAAGAACC CTGTTTACAT GGTGCGCAGT CACAGAGTTC ACACTAACAA CTGCCCTACT 
GTCGTCAGCC AGATCAATCA GCAGCACACG CACCCCAGTC CTCAGCATCA AGGACAACTG 
AGTCTGGACC TAAGCCAGAA ACGCTCCACG GACTATTCTG AGTCGTCCCG TAGCAGCCGG 
GCCTCACACG GCACCAACTC CCTGCCGTCC AGTGCAAGAC TCGGTTCATC AAACAACTTG 
CAGTATCGCA CTGAAAGGAT TAAGATTCCC TCCACACCGC GATATCCTCG CTCTATTCTG 
GGGTCAGACA GAGGGTCCCT GTCTCATTCA GAATGCAGCA GTCCCAGTCT CATTACTCCC 
CCTCTTTCTC CTCTCAATCT GGAGACGTCT TCGTTTGCCT CCAGCCAGTC TCAGGGCTCC 
ATCTCCACCC TGCCCAGGAT CTCCGTCAGC CCCGTACCCA CAGAAGAGCG CAGAAAAGAC 
AGGCCGTACT TGGAGGAGCC ACGGAATGTT ATGGTGCACA AAGGGGCGGA GCCTCTCGGC 
ATCTCCATTG TGAGTGGAGA GAACGGAGGA ATCTTTGTCT CCAAGGTGAC AGGAGGCAGT 
ATCGCGCACC AGGCTGGGTT GGAGTATGGG GACCAGCTAT TGGAGTATAA TGGAATAAAC 
CTGCGCAATG CCACTGAACA GCAAGCACGA CTCATCATTG GCCAGCAGTG TGACACCATC 
ACGATCATGG CCCAATACAA CCCACACATG TATCAGCTCG GCAACCACTC GCGCTCCAGT 
TCCCGCCTGG AGCCAGTGAG CACTCAATCC ACGCCACAGG GCAGTGGAAC TGCAACCCCA 
GATAACCACT CTACCATAGA CACCCTGAGT GAACAGGATG AAGGCACACT CACCCCATCC 
TCCAAACAGA CCACACCCAC AACCAGTCCC AACAGCTTCA TCAGAATGCC GTCTGAAAGT 
TCCAAGAAAG TACCAGAGGC GCGTTTGGTG ACAGTGAGGA AGACTCAGGT GGAGCTGGGC 
ATTCAGCTGT GTGGAGGAAA CCTGCGGGGA ATCTTCGTGG AGAGGCTGGA AGATGACAGT 
CCTGCCAGAG GAGCGGATGC TGTTGTACCC GGGGACATGA TACTTGAGTA TGGCTCAGTT 
AATATGAAGA ATAAGACTGT GGAGGAGGCA TATCTGGAAA TGCTGAAGCC TGCAGAAACA 
GTGACGTTAA GAGTCCAGCA TCGTCTGGAT GAGTTCAACC AGGTGAAAGG CACTCCTGGA 
GATGGATTCT ATATCAGAGC ACTTTATGAC CGAGTAGCTG AGACTGAGCT CGACCTCTCT 
TTTAAGAAAG ACGACATCCT CTATGTGGAC GACACACTAC CAAATGGCAA CTTCGGCACC 
TGGATGGCCT GGCAACTTGA CGAAAATGCA CAAAAAATTC AAAGAGGCCA GATTCCTAGC 
AAATTTATGA TGGATCAGGA GTTTTACCGT AGACACAGTA TGACTGAACT GAAGGACGAA 
ACGGGCTCCA GTAAAACCCT CTCGGCTGCT GCACGCAGAT CCTTCTTCCG CAGGAAACAC 
AAGCACAAAC GCAGCAGCTC CAAAGACGGG AAAGACGTAG TGGCTTTGGA CGCCATCAGC 
ACAGACTCAA TTCCTTTCTT AGACGACTGT GTGAGTCTGG CTTATCAGAG AGTGCAGAAG 
ATTGACTGCA CATCTCCCAG ACCTGTGCTG ATCCTCGGCC CGCTTGTGGA TCCTGTTAAA 
GACATGCTGG TCAAAGATTC TCCAGGGAAA TTCAGCAGAT GTGTTCTTGA GGTGATGAAG 
GCTTCTCAGC AGGCCATTGA GCGTGGGGTC AAAGACTTTC TCTTCATCGA CTACAAACGA 
AGGAGTGGCC ATTTTGATGT GACGACTGTT GCCTCAATCA ATGAAATCAC GGACAAGGAC 
TGTCACTGTT TGCTTGACAT TGCACCTCAT GCTATTGAAA GGCTTCACAG CGTTCACATT 
TACCCAATCG TCATTTTCAT ACGCTACAAA AACGCAAAGC AGATAAAAGA GCAGAAGGAC 
CCGATTTATC TGCGGGATAA AGTCTCTCAA AAACATTCCA AGGAGCAGTT TGAGATTGCA 
CAGAAGATTG AGCAAGACTA CAGCAAGTTC TTCACAGGTA TCGTTCAGGG AGGCACCTTC 
CCTTACATTT GCACTCAGAT CGTGACTATT GTGGATCAGG AACAGAGCAA AGTCTTGTGG 
ACTCCACTGG GCTCTCCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_005156681.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156738.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.

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CloneID	ODa18634
Clone ID Related Accession (Same CDS sequence)	XM_005156681.4 , XM_005156681.3
Accession Version	XM_005156681.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disks large homolog 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156681.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGGCC GTACTTGGAG 
GAGCCACGGA ATGTTATGGT GCACAAAGGG GCGGAGCCTC TCGGCATCTC CATTGTGAGT 
GGAGAGAACG GAGGAATCTT TGTCTCCAAG GTGACAGGAG GCAGTATCGC GCACCAGGCT 
GGGTTGGAGT ATGGGGACCA GCTATTGGAG TATAATGGAA TAAACCTGCG CAATGCCACT 
GAACAGCAAG CACGACTCAT CATTGGCCAG CAGTGTGACA CCATCACGAT CATGGCCCAA 
TACAACCCAC ACATGTATCA GCTCGGCAAC CACTCGCGCT CCAGTTCCCG CCTGGAGCCA 
GTGAGCACTC AATCCACGCC ACAGGGCAGT GGAACTGCAA CCCCAGATAA CCACTCTACC 
ATAGACACCC TGAGTGAACA GGATGAAGGC ACACTCACCC CATCCTCCAA ACAGACCACA 
CCCACAACCA GTCCCAACAG CTTCATCAGA ATGCCGTCTG AAAGTTCCAA GAAAGTACCA 
GAGGCGCGTT TGGTGACAGT GAGGAAGACT CAGGTGGAGC TGGGCATTCA GCTGTGTGGA 
GGAAACCTGC GGGGAATCTT CGTGGAGAGG CTGGAAGATG ACAGTCCTGC CAGAGGAGCG 
GATGCTGTTG TACCCGGGGA CATGATACTT GAGTATGGCT CAGTTAATAT GAAGAATAAG 
ACTGTGGAGG AGGCATATCT GGAAATGCTG AAGCCTGCAG AAACAGTGAC GTTAAGAGTC 
CAGCATCGTC TGGATGAGTT CAACCAGGTG AAAGGCACTC CTGGAGATGG ATTCTATATC 
AGAGCACTTT ATGACCGAGT AGCTGAGACT GAGCTCGACC TCTCTTTTAA GAAAGACGAC 
ATCCTCTATG TGGACGACAC ACTACCAAAT GGCAACTTCG GCACCTGGAT GGCCTGGCAA 
CTTGACGAAA ATGCACAAAA AATTCAAAGA GGCCAGATTC CTAGCAAATT TATGATGGAT 
CAGGAGTTTT ACCGTAGACA CAGTATGACT GAACTGAAGG ACGAAACGGG CTCCAGTAAA 
ACCCTCTCGG CTGCTGCACG CAGATCCTTC TTCCGCAGGA AACACAAGCA CAAACGCAGC 
AGCTCCAAAG ACGGGAAAGA CGTAGTGGCT TTGGACGCCA TCAGCACAGA CTCAATTCCT 
TTCTTAGACG ACTGTGTGAG TCTGGCTTAT CAGAGAGTGC AGAAGATTGA CTGCACATCT 
CCCAGACCTG TGCTGATCCT CGGCCCGCTT GTGGATCCTG TTAAAGACAT GCTGGTCAAA 
GATTCTCCAG GGAAATTCAG CAGATGTGTT CTTGAGGTGA TGAAGGCTTC TCAGCAGGCC 
ATTGAGCGTG GGGTCAAAGA CTTTCTCTTC ATCGACTACA AACGAAGGAG TGGCCATTTT 
GATGTGACGA CTGTTGCCTC AATCAATGAA ATCACGGACA AGGACTGTCA CTGTTTGCTT 
GACATTGCAC CTCATGCTAT TGAAAGGCTT CACAGCGTTC ACATTTACCC AATCGTCATT 
TTCATACGCT ACAAAAACGC AAAGCAGATA AAAGAGCAGA AGGACCCGAT TTATCTGCGG 
GATAAAGTCT CTCAAAAACA TTCCAAGGAG CAGTTTGAGA TTGCACAGAA GATTGAGCAA 
GACTACAGCA AGTTCTTCAC AGGTATCGTT CAGGGAGGCA CCTTCCCTTA CATTTGCACT 
CAGATCGTGA CTATTGTGGA TCAGGAACAG AGCAAAGTCT TGTGGACTCC ACTGGGCTCT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156738.1
CDS	976..6861
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_005156681.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156681.4

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGGCC GTACTTGGAG 
GAGCCACGGA ATGTTATGGT GCACAAAGGG GCGGAGCCTC TCGGCATCTC CATTGTGAGT 
GGAGAGAACG GAGGAATCTT TGTCTCCAAG GTGACAGGAG GCAGTATCGC GCACCAGGCT 
GGGTTGGAGT ATGGGGACCA GCTATTGGAG TATAATGGAA TAAACCTGCG CAATGCCACT 
GAACAGCAAG CACGACTCAT CATTGGCCAG CAGTGTGACA CCATCACGAT CATGGCCCAA 
TACAACCCAC ACATGTATCA GCTCGGCAAC CACTCGCGCT CCAGTTCCCG CCTGGAGCCA 
GTGAGCACTC AATCCACGCC ACAGGGCAGT GGAACTGCAA CCCCAGATAA CCACTCTACC 
ATAGACACCC TGAGTGAACA GGATGAAGGC ACACTCACCC CATCCTCCAA ACAGACCACA 
CCCACAACCA GTCCCAACAG CTTCATCAGA ATGCCGTCTG AAAGTTCCAA GAAAGTACCA 
GAGGCGCGTT TGGTGACAGT GAGGAAGACT CAGGTGGAGC TGGGCATTCA GCTGTGTGGA 
GGAAACCTGC GGGGAATCTT CGTGGAGAGG CTGGAAGATG ACAGTCCTGC CAGAGGAGCG 
GATGCTGTTG TACCCGGGGA CATGATACTT GAGTATGGCT CAGTTAATAT GAAGAATAAG 
ACTGTGGAGG AGGCATATCT GGAAATGCTG AAGCCTGCAG AAACAGTGAC GTTAAGAGTC 
CAGCATCGTC TGGATGAGTT CAACCAGGTG AAAGGCACTC CTGGAGATGG ATTCTATATC 
AGAGCACTTT ATGACCGAGT AGCTGAGACT GAGCTCGACC TCTCTTTTAA GAAAGACGAC 
ATCCTCTATG TGGACGACAC ACTACCAAAT GGCAACTTCG GCACCTGGAT GGCCTGGCAA 
CTTGACGAAA ATGCACAAAA AATTCAAAGA GGCCAGATTC CTAGCAAATT TATGATGGAT 
CAGGAGTTTT ACCGTAGACA CAGTATGACT GAACTGAAGG ACGAAACGGG CTCCAGTAAA 
ACCCTCTCGG CTGCTGCACG CAGATCCTTC TTCCGCAGGA AACACAAGCA CAAACGCAGC 
AGCTCCAAAG ACGGGAAAGA CGTAGTGGCT TTGGACGCCA TCAGCACAGA CTCAATTCCT 
TTCTTAGACG ACTGTGTGAG TCTGGCTTAT CAGAGAGTGC AGAAGATTGA CTGCACATCT 
CCCAGACCTG TGCTGATCCT CGGCCCGCTT GTGGATCCTG TTAAAGACAT GCTGGTCAAA 
GATTCTCCAG GGAAATTCAG CAGATGTGTT CTTGAGGTGA TGAAGGCTTC TCAGCAGGCC 
ATTGAGCGTG GGGTCAAAGA CTTTCTCTTC ATCGACTACA AACGAAGGAG TGGCCATTTT 
GATGTGACGA CTGTTGCCTC AATCAATGAA ATCACGGACA AGGACTGTCA CTGTTTGCTT 
GACATTGCAC CTCATGCTAT TGAAAGGCTT CACAGCGTTC ACATTTACCC AATCGTCATT 
TTCATACGCT ACAAAAACGC AAAGCAGATA AAAGAGCAGA AGGACCCGAT TTATCTGCGG 
GATAAAGTCT CTCAAAAACA TTCCAAGGAG CAGTTTGAGA TTGCACAGAA GATTGAGCAA 
GACTACAGCA AGTTCTTCAC AGGTATCGTT CAGGGAGGCA CCTTCCCTTA CATTTGCACT 
CAGATCGTGA CTATTGTGGA TCAGGAACAG AGCAAAGTCT TGTGGACTCC ACTGGGCTCT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_005156681.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156738.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.

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CloneID	ODa18634
Clone ID Related Accession (Same CDS sequence)	XM_005156681.4 , XM_005156681.3
Accession Version	XM_005156681.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disks large homolog 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156681.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGGCC GTACTTGGAG 
GAGCCACGGA ATGTTATGGT GCACAAAGGG GCGGAGCCTC TCGGCATCTC CATTGTGAGT 
GGAGAGAACG GAGGAATCTT TGTCTCCAAG GTGACAGGAG GCAGTATCGC GCACCAGGCT 
GGGTTGGAGT ATGGGGACCA GCTATTGGAG TATAATGGAA TAAACCTGCG CAATGCCACT 
GAACAGCAAG CACGACTCAT CATTGGCCAG CAGTGTGACA CCATCACGAT CATGGCCCAA 
TACAACCCAC ACATGTATCA GCTCGGCAAC CACTCGCGCT CCAGTTCCCG CCTGGAGCCA 
GTGAGCACTC AATCCACGCC ACAGGGCAGT GGAACTGCAA CCCCAGATAA CCACTCTACC 
ATAGACACCC TGAGTGAACA GGATGAAGGC ACACTCACCC CATCCTCCAA ACAGACCACA 
CCCACAACCA GTCCCAACAG CTTCATCAGA ATGCCGTCTG AAAGTTCCAA GAAAGTACCA 
GAGGCGCGTT TGGTGACAGT GAGGAAGACT CAGGTGGAGC TGGGCATTCA GCTGTGTGGA 
GGAAACCTGC GGGGAATCTT CGTGGAGAGG CTGGAAGATG ACAGTCCTGC CAGAGGAGCG 
GATGCTGTTG TACCCGGGGA CATGATACTT GAGTATGGCT CAGTTAATAT GAAGAATAAG 
ACTGTGGAGG AGGCATATCT GGAAATGCTG AAGCCTGCAG AAACAGTGAC GTTAAGAGTC 
CAGCATCGTC TGGATGAGTT CAACCAGGTG AAAGGCACTC CTGGAGATGG ATTCTATATC 
AGAGCACTTT ATGACCGAGT AGCTGAGACT GAGCTCGACC TCTCTTTTAA GAAAGACGAC 
ATCCTCTATG TGGACGACAC ACTACCAAAT GGCAACTTCG GCACCTGGAT GGCCTGGCAA 
CTTGACGAAA ATGCACAAAA AATTCAAAGA GGCCAGATTC CTAGCAAATT TATGATGGAT 
CAGGAGTTTT ACCGTAGACA CAGTATGACT GAACTGAAGG ACGAAACGGG CTCCAGTAAA 
ACCCTCTCGG CTGCTGCACG CAGATCCTTC TTCCGCAGGA AACACAAGCA CAAACGCAGC 
AGCTCCAAAG ACGGGAAAGA CGTAGTGGCT TTGGACGCCA TCAGCACAGA CTCAATTCCT 
TTCTTAGACG ACTGTGTGAG TCTGGCTTAT CAGAGAGTGC AGAAGATTGA CTGCACATCT 
CCCAGACCTG TGCTGATCCT CGGCCCGCTT GTGGATCCTG TTAAAGACAT GCTGGTCAAA 
GATTCTCCAG GGAAATTCAG CAGATGTGTT CTTGAGGTGA TGAAGGCTTC TCAGCAGGCC 
ATTGAGCGTG GGGTCAAAGA CTTTCTCTTC ATCGACTACA AACGAAGGAG TGGCCATTTT 
GATGTGACGA CTGTTGCCTC AATCAATGAA ATCACGGACA AGGACTGTCA CTGTTTGCTT 
GACATTGCAC CTCATGCTAT TGAAAGGCTT CACAGCGTTC ACATTTACCC AATCGTCATT 
TTCATACGCT ACAAAAACGC AAAGCAGATA AAAGAGCAGA AGGACCCGAT TTATCTGCGG 
GATAAAGTCT CTCAAAAACA TTCCAAGGAG CAGTTTGAGA TTGCACAGAA GATTGAGCAA 
GACTACAGCA AGTTCTTCAC AGGTATCGTT CAGGGAGGCA CCTTCCCTTA CATTTGCACT 
CAGATCGTGA CTATTGTGGA TCAGGAACAG AGCAAAGTCT TGTGGACTCC ACTGGGCTCT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156738.1
CDS	997..6882
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_005156681.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156681.3

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG TTCTTTCCCC AGAGCACATC AGGACAGAAC 
ATCTTCGAGA GCCTGAGGGA GGCAGAGAAA TCCAATGGCA GAATCCACCT GACAGATGTG 
CACTCACGAA ACAGCAGAAA CCTCAAACAC AACAGCTCCA CCCAGACAGA CATTTACAGC 
TGTGACACGG CCGGAGAGAG ACGAAAAACC AGATCTGATG CAGAGGAGAG CCTTTTTTCT 
GAAAGGAAGG TCTTCCCCAT ATCCACCTTG CACCCAAACA CACTGCGACC TGCTTCAGAA 
CTGGGACCAG TCCGTTACAG CGCCAGCGCC TTTCAGGAGG TCTGCTACAC TCGCTCAGAG 
TCCGTCGGCC CTGAATGTGT AACGTTGGAG ACCACCTTAG AGAAGAAACA TAGTGGCGGC 
ACTTGGCCCA AAATGGTGGT TGGTGTCGCG ATGACCACGG ACAGCCCAGC GCAGCTTTCC 
ATCTACAGGT CACCCAAACA AAGAAAGTCC ATCTTTGATG CCGATACCTT CAAACGACCT 
GATACCCCCT CAAAAGCTGA TTACCATGGC CCTCAGCCTT TAAAAGCAGA CTCCGCCCCC 
ACACCCCCAA CCCCGCCTAC TCGCAGCGAC TCCTTTAAAT TCAAGCACAA ACATCAGGGA 
AGCTCCACCT CCGAGTCCAC CATTAGTGCC ATGTCCATCT CTGAACCTAA GCAGGAAGAT 
CGTAATGGGA ACTTGTACTT CAGTGAGGGT AAAGTCTTGA GCTCCAGGAA GTCCTGTGAG 
GAGGACATCG GTCGGATGTG GGCGGAGGAG CCCGAAGTGA AGCGTCCCAG GCCCAAATCG 
GCCCCGGCCC TCAGACGCAG GATGACTCCT CAAAACATTC CTCTCCAGAG CTTCCAGAGT 
TACTCTAATG ACGGTGACTC CGTGGAGCAG AGGGAGATGC TGCGCTCCTC TCCTAACCGA 
CCTCACAGAC ACAGCGTGGG TTTCGTGCCT ACGTCCTATA ATGGAACCCT TCCTCCCAAC 
TCAGCACATC GAGGACTGGC CCCCTGCTCT GCAGTAACAG CCGTCATGAA GAACCCTGTT 
TACATGGTGC GCAGTCACAG AGTTCACACT AACAACTGCC CTACTGTCGT CAGCCAGATC 
AATCAGCAGC ACACGCACCC CAGTCCTCAG CATCAAGGAC AACTGAGTCT GGACCTAAGC 
CAGAAACGCT CCACGGACTA TTCTGAGTCG TCCCGTAGCA GCCGGGCCTC ACACGGCACC 
AACTCCCTGC CGTCCAGTGC AAGACTCGGT TCATCAAACA ACTTGCAGTA TCGCACTGAA 
AGGATTAAGA TTCCCTCCAC ACCGCGATAT CCTCGCTCTA TTCTGGGGTC AGACAGAGGG 
TCCCTGTCTC ATTCAGAATG CAGCAGTCCC AGTCTCATTA CTCCCCCTCT TTCTCCTCTC 
AATCTGGAGA CGTCTTCGTT TGCCTCCAGC CAGTCTCAGG GCTCCATCTC CACCCTGCCC 
AGGATCTCCG TCAGCCCCGT ACCCACAGAA GAGCGCAGAA AAGACAGGCC GTACTTGGAG 
GAGCCACGGA ATGTTATGGT GCACAAAGGG GCGGAGCCTC TCGGCATCTC CATTGTGAGT 
GGAGAGAACG GAGGAATCTT TGTCTCCAAG GTGACAGGAG GCAGTATCGC GCACCAGGCT 
GGGTTGGAGT ATGGGGACCA GCTATTGGAG TATAATGGAA TAAACCTGCG CAATGCCACT 
GAACAGCAAG CACGACTCAT CATTGGCCAG CAGTGTGACA CCATCACGAT CATGGCCCAA 
TACAACCCAC ACATGTATCA GCTCGGCAAC CACTCGCGCT CCAGTTCCCG CCTGGAGCCA 
GTGAGCACTC AATCCACGCC ACAGGGCAGT GGAACTGCAA CCCCAGATAA CCACTCTACC 
ATAGACACCC TGAGTGAACA GGATGAAGGC ACACTCACCC CATCCTCCAA ACAGACCACA 
CCCACAACCA GTCCCAACAG CTTCATCAGA ATGCCGTCTG AAAGTTCCAA GAAAGTACCA 
GAGGCGCGTT TGGTGACAGT GAGGAAGACT CAGGTGGAGC TGGGCATTCA GCTGTGTGGA 
GGAAACCTGC GGGGAATCTT CGTGGAGAGG CTGGAAGATG ACAGTCCTGC CAGAGGAGCG 
GATGCTGTTG TACCCGGGGA CATGATACTT GAGTATGGCT CAGTTAATAT GAAGAATAAG 
ACTGTGGAGG AGGCATATCT GGAAATGCTG AAGCCTGCAG AAACAGTGAC GTTAAGAGTC 
CAGCATCGTC TGGATGAGTT CAACCAGGTG AAAGGCACTC CTGGAGATGG ATTCTATATC 
AGAGCACTTT ATGACCGAGT AGCTGAGACT GAGCTCGACC TCTCTTTTAA GAAAGACGAC 
ATCCTCTATG TGGACGACAC ACTACCAAAT GGCAACTTCG GCACCTGGAT GGCCTGGCAA 
CTTGACGAAA ATGCACAAAA AATTCAAAGA GGCCAGATTC CTAGCAAATT TATGATGGAT 
CAGGAGTTTT ACCGTAGACA CAGTATGACT GAACTGAAGG ACGAAACGGG CTCCAGTAAA 
ACCCTCTCGG CTGCTGCACG CAGATCCTTC TTCCGCAGGA AACACAAGCA CAAACGCAGC 
AGCTCCAAAG ACGGGAAAGA CGTAGTGGCT TTGGACGCCA TCAGCACAGA CTCAATTCCT 
TTCTTAGACG ACTGTGTGAG TCTGGCTTAT CAGAGAGTGC AGAAGATTGA CTGCACATCT 
CCCAGACCTG TGCTGATCCT CGGCCCGCTT GTGGATCCTG TTAAAGACAT GCTGGTCAAA 
GATTCTCCAG GGAAATTCAG CAGATGTGTT CTTGAGGTGA TGAAGGCTTC TCAGCAGGCC 
ATTGAGCGTG GGGTCAAAGA CTTTCTCTTC ATCGACTACA AACGAAGGAG TGGCCATTTT 
GATGTGACGA CTGTTGCCTC AATCAATGAA ATCACGGACA AGGACTGTCA CTGTTTGCTT 
GACATTGCAC CTCATGCTAT TGAAAGGCTT CACAGCGTTC ACATTTACCC AATCGTCATT 
TTCATACGCT ACAAAAACGC AAAGCAGATA AAAGAGCAGA AGGACCCGAT TTATCTGCGG 
GATAAAGTCT CTCAAAAACA TTCCAAGGAG CAGTTTGAGA TTGCACAGAA GATTGAGCAA 
GACTACAGCA AGTTCTTCAC AGGTATCGTT CAGGGAGGCA CCTTCCCTTA CATTTGCACT 
CAGATCGTGA CTATTGTGGA TCAGGAACAG AGCAAAGTCT TGTGGACTCC ACTGGGCTCT 
CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_005156682.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156739.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.

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CloneID	ODa18635
Clone ID Related Accession (Same CDS sequence)	XM_005156682.4 , XM_005156682.3
Accession Version	XM_005156682.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disks large homolog 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156682.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAA CTCAGCACAT 
CGAGGACTGG CCCCCTGCTC TGCAGTAACA GCCGTCATGA AGAACCCTGT TTACATGGTG 
CGCAGTCACA GAGTTCACAC TAACAACTGC CCTACTGTCG TCAGCCAGAT CAATCAGCAG 
CACACGCACC CCAGTCCTCA GCATCAAGGA CAACTGAGTC TGGACCTAAG CCAGAAACGC 
TCCACGGACT ATTCTGAGTC GTCCCGTAGC AGCCGGGCCT CACACGGCAC CAACTCCCTG 
CCGTCCAGTG CAAGACTCGG TTCATCAAAC AACTTGCAGT ATCGCACTGA AAGGATTAAG 
ATTCCCTCCA CACCGCGATA TCCTCGCTCT ATTCTGGGGT CAGACAGAGG GTCCCTGTCT 
CATTCAGAAT GCAGCAGTCC CAGTCTCATT ACTCCCCCTC TTTCTCCTCT CAATCTGGAG 
ACGTCTTCGT TTGCCTCCAG CCAGTCTCAG GGCTCCATCT CCACCCTGCC CAGGATCTCC 
GTCAGCCCCG TACCCACAGA AGAGCGCAGA AAAGACAGGC CGTACTTGGA GGAGCCACGG 
AATGTTATGG TGCACAAAGG GGCGGAGCCT CTCGGCATCT CCATTGTGAG TGGAGAGAAC 
GGAGGAATCT TTGTCTCCAA GGTGACAGGA GGCAGTATCG CGCACCAGGC TGGGTTGGAG 
TATGGGGACC AGCTATTGGA GTATAATGGA ATAAACCTGC GCAATGCCAC TGAACAGCAA 
GCACGACTCA TCATTGGCCA GCAGTGTGAC ACCATCACGA TCATGGCCCA ATACAACCCA 
CACATGTATC AGCTCGGCAA CCACTCGCGC TCCAGTTCCC GCCTGGAGCC AGTGAGCACT 
CAATCCACGC CACAGGGCAG TGGAACTGCA ACCCCAGATA ACCACTCTAC CATAGACACC 
CTGAGTGAAC AGGATGAAGG CACACTCACC CCATCCTCCA AACAGACCAC ACCCACAACC 
AGTCCCAACA GCTTCATCAG AATGCCGTCT GAAAGTTCCA AGAAAGTACC AGAGGCGCGT 
TTGGTGACAG TGAGGAAGAC TCAGGTGGAG CTGGGCATTC AGCTGTGTGG AGGAAACCTG 
CGGGGAATCT TCGTGGAGAG GCTGGAAGAT GACAGTCCTG CCAGAGGAGC GGATGCTGTT 
GTACCCGGGG ACATGATACT TGAGTATGGC TCAGTTAATA TGAAGAATAA GACTGTGGAG 
GAGGCATATC TGGAAATGCT GAAGCCTGCA GAAACAGTGA CGTTAAGAGT CCAGCATCGT 
CTGGATGAGT TCAACCAGGT GAAAGGCACT CCTGGAGATG GATTCTATAT CAGAGCACTT 
TATGACCGAG TAGCTGAGAC TGAGCTCGAC CTCTCTTTTA AGAAAGACGA CATCCTCTAT 
GTGGACGACA CACTACCAAA TGGCAACTTC GGCACCTGGA TGGCCTGGCA ACTTGACGAA 
AATGCACAAA AAATTCAAAG AGGCCAGATT CCTAGCAAAT TTATGATGGA TCAGGAGTTT 
TACCGTAGAC ACAGTATGAC TGAACTGAAG GACGAAACGG GCTCCAGTAA AACCCTCTCG 
GCTGCTGCAC GCAGATCCTT CTTCCGCAGG AAACACAAGC ACAAACGCAG CAGCTCCAAA 
GACGGGAAAG ACGTAGTGGC TTTGGACGCC ATCAGCACAG ACTCAATTCC TTTCTTAGAC 
GACTGTGTGA GTCTGGCTTA TCAGAGAGTG CAGAAGATTG ACTGCACATC TCCCAGACCT 
GTGCTGATCC TCGGCCCGCT TGTGGATCCT GTTAAAGACA TGCTGGTCAA AGATTCTCCA 
GGGAAATTCA GCAGATGTGT TCTTGAGGTG ATGAAGGCTT CTCAGCAGGC CATTGAGCGT 
GGGGTCAAAG ACTTTCTCTT CATCGACTAC AAACGAAGGA GTGGCCATTT TGATGTGACG 
ACTGTTGCCT CAATCAATGA AATCACGGAC AAGGACTGTC ACTGTTTGCT TGACATTGCA 
CCTCATGCTA TTGAAAGGCT TCACAGCGTT CACATTTACC CAATCGTCAT TTTCATACGC 
TACAAAAACG CAAAGCAGAT AAAAGAGCAG AAGGACCCGA TTTATCTGCG GGATAAAGTC 
TCTCAAAAAC ATTCCAAGGA GCAGTTTGAG ATTGCACAGA AGATTGAGCA AGACTACAGC 
AAGTTCTTCA CAGGTATCGT TCAGGGAGGC ACCTTCCCTT ACATTTGCAC TCAGATCGTG 
ACTATTGTGG ATCAGGAACA GAGCAAAGTC TTGTGGACTC CACTGGGCTC TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156739.1
CDS	976..6852
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_005156682.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156682.4

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAA CTCAGCACAT 
CGAGGACTGG CCCCCTGCTC TGCAGTAACA GCCGTCATGA AGAACCCTGT TTACATGGTG 
CGCAGTCACA GAGTTCACAC TAACAACTGC CCTACTGTCG TCAGCCAGAT CAATCAGCAG 
CACACGCACC CCAGTCCTCA GCATCAAGGA CAACTGAGTC TGGACCTAAG CCAGAAACGC 
TCCACGGACT ATTCTGAGTC GTCCCGTAGC AGCCGGGCCT CACACGGCAC CAACTCCCTG 
CCGTCCAGTG CAAGACTCGG TTCATCAAAC AACTTGCAGT ATCGCACTGA AAGGATTAAG 
ATTCCCTCCA CACCGCGATA TCCTCGCTCT ATTCTGGGGT CAGACAGAGG GTCCCTGTCT 
CATTCAGAAT GCAGCAGTCC CAGTCTCATT ACTCCCCCTC TTTCTCCTCT CAATCTGGAG 
ACGTCTTCGT TTGCCTCCAG CCAGTCTCAG GGCTCCATCT CCACCCTGCC CAGGATCTCC 
GTCAGCCCCG TACCCACAGA AGAGCGCAGA AAAGACAGGC CGTACTTGGA GGAGCCACGG 
AATGTTATGG TGCACAAAGG GGCGGAGCCT CTCGGCATCT CCATTGTGAG TGGAGAGAAC 
GGAGGAATCT TTGTCTCCAA GGTGACAGGA GGCAGTATCG CGCACCAGGC TGGGTTGGAG 
TATGGGGACC AGCTATTGGA GTATAATGGA ATAAACCTGC GCAATGCCAC TGAACAGCAA 
GCACGACTCA TCATTGGCCA GCAGTGTGAC ACCATCACGA TCATGGCCCA ATACAACCCA 
CACATGTATC AGCTCGGCAA CCACTCGCGC TCCAGTTCCC GCCTGGAGCC AGTGAGCACT 
CAATCCACGC CACAGGGCAG TGGAACTGCA ACCCCAGATA ACCACTCTAC CATAGACACC 
CTGAGTGAAC AGGATGAAGG CACACTCACC CCATCCTCCA AACAGACCAC ACCCACAACC 
AGTCCCAACA GCTTCATCAG AATGCCGTCT GAAAGTTCCA AGAAAGTACC AGAGGCGCGT 
TTGGTGACAG TGAGGAAGAC TCAGGTGGAG CTGGGCATTC AGCTGTGTGG AGGAAACCTG 
CGGGGAATCT TCGTGGAGAG GCTGGAAGAT GACAGTCCTG CCAGAGGAGC GGATGCTGTT 
GTACCCGGGG ACATGATACT TGAGTATGGC TCAGTTAATA TGAAGAATAA GACTGTGGAG 
GAGGCATATC TGGAAATGCT GAAGCCTGCA GAAACAGTGA CGTTAAGAGT CCAGCATCGT 
CTGGATGAGT TCAACCAGGT GAAAGGCACT CCTGGAGATG GATTCTATAT CAGAGCACTT 
TATGACCGAG TAGCTGAGAC TGAGCTCGAC CTCTCTTTTA AGAAAGACGA CATCCTCTAT 
GTGGACGACA CACTACCAAA TGGCAACTTC GGCACCTGGA TGGCCTGGCA ACTTGACGAA 
AATGCACAAA AAATTCAAAG AGGCCAGATT CCTAGCAAAT TTATGATGGA TCAGGAGTTT 
TACCGTAGAC ACAGTATGAC TGAACTGAAG GACGAAACGG GCTCCAGTAA AACCCTCTCG 
GCTGCTGCAC GCAGATCCTT CTTCCGCAGG AAACACAAGC ACAAACGCAG CAGCTCCAAA 
GACGGGAAAG ACGTAGTGGC TTTGGACGCC ATCAGCACAG ACTCAATTCC TTTCTTAGAC 
GACTGTGTGA GTCTGGCTTA TCAGAGAGTG CAGAAGATTG ACTGCACATC TCCCAGACCT 
GTGCTGATCC TCGGCCCGCT TGTGGATCCT GTTAAAGACA TGCTGGTCAA AGATTCTCCA 
GGGAAATTCA GCAGATGTGT TCTTGAGGTG ATGAAGGCTT CTCAGCAGGC CATTGAGCGT 
GGGGTCAAAG ACTTTCTCTT CATCGACTAC AAACGAAGGA GTGGCCATTT TGATGTGACG 
ACTGTTGCCT CAATCAATGA AATCACGGAC AAGGACTGTC ACTGTTTGCT TGACATTGCA 
CCTCATGCTA TTGAAAGGCT TCACAGCGTT CACATTTACC CAATCGTCAT TTTCATACGC 
TACAAAAACG CAAAGCAGAT AAAAGAGCAG AAGGACCCGA TTTATCTGCG GGATAAAGTC 
TCTCAAAAAC ATTCCAAGGA GCAGTTTGAG ATTGCACAGA AGATTGAGCA AGACTACAGC 
AAGTTCTTCA CAGGTATCGT TCAGGGAGGC ACCTTCCCTT ACATTTGCAC TCAGATCGTG 
ACTATTGTGG ATCAGGAACA GAGCAAAGTC TTGTGGACTC CACTGGGCTC TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_005156682.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156739.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.

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CloneID	ODa18635
Clone ID Related Accession (Same CDS sequence)	XM_005156682.4 , XM_005156682.3
Accession Version	XM_005156682.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disks large homolog 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156682.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAA CTCAGCACAT 
CGAGGACTGG CCCCCTGCTC TGCAGTAACA GCCGTCATGA AGAACCCTGT TTACATGGTG 
CGCAGTCACA GAGTTCACAC TAACAACTGC CCTACTGTCG TCAGCCAGAT CAATCAGCAG 
CACACGCACC CCAGTCCTCA GCATCAAGGA CAACTGAGTC TGGACCTAAG CCAGAAACGC 
TCCACGGACT ATTCTGAGTC GTCCCGTAGC AGCCGGGCCT CACACGGCAC CAACTCCCTG 
CCGTCCAGTG CAAGACTCGG TTCATCAAAC AACTTGCAGT ATCGCACTGA AAGGATTAAG 
ATTCCCTCCA CACCGCGATA TCCTCGCTCT ATTCTGGGGT CAGACAGAGG GTCCCTGTCT 
CATTCAGAAT GCAGCAGTCC CAGTCTCATT ACTCCCCCTC TTTCTCCTCT CAATCTGGAG 
ACGTCTTCGT TTGCCTCCAG CCAGTCTCAG GGCTCCATCT CCACCCTGCC CAGGATCTCC 
GTCAGCCCCG TACCCACAGA AGAGCGCAGA AAAGACAGGC CGTACTTGGA GGAGCCACGG 
AATGTTATGG TGCACAAAGG GGCGGAGCCT CTCGGCATCT CCATTGTGAG TGGAGAGAAC 
GGAGGAATCT TTGTCTCCAA GGTGACAGGA GGCAGTATCG CGCACCAGGC TGGGTTGGAG 
TATGGGGACC AGCTATTGGA GTATAATGGA ATAAACCTGC GCAATGCCAC TGAACAGCAA 
GCACGACTCA TCATTGGCCA GCAGTGTGAC ACCATCACGA TCATGGCCCA ATACAACCCA 
CACATGTATC AGCTCGGCAA CCACTCGCGC TCCAGTTCCC GCCTGGAGCC AGTGAGCACT 
CAATCCACGC CACAGGGCAG TGGAACTGCA ACCCCAGATA ACCACTCTAC CATAGACACC 
CTGAGTGAAC AGGATGAAGG CACACTCACC CCATCCTCCA AACAGACCAC ACCCACAACC 
AGTCCCAACA GCTTCATCAG AATGCCGTCT GAAAGTTCCA AGAAAGTACC AGAGGCGCGT 
TTGGTGACAG TGAGGAAGAC TCAGGTGGAG CTGGGCATTC AGCTGTGTGG AGGAAACCTG 
CGGGGAATCT TCGTGGAGAG GCTGGAAGAT GACAGTCCTG CCAGAGGAGC GGATGCTGTT 
GTACCCGGGG ACATGATACT TGAGTATGGC TCAGTTAATA TGAAGAATAA GACTGTGGAG 
GAGGCATATC TGGAAATGCT GAAGCCTGCA GAAACAGTGA CGTTAAGAGT CCAGCATCGT 
CTGGATGAGT TCAACCAGGT GAAAGGCACT CCTGGAGATG GATTCTATAT CAGAGCACTT 
TATGACCGAG TAGCTGAGAC TGAGCTCGAC CTCTCTTTTA AGAAAGACGA CATCCTCTAT 
GTGGACGACA CACTACCAAA TGGCAACTTC GGCACCTGGA TGGCCTGGCA ACTTGACGAA 
AATGCACAAA AAATTCAAAG AGGCCAGATT CCTAGCAAAT TTATGATGGA TCAGGAGTTT 
TACCGTAGAC ACAGTATGAC TGAACTGAAG GACGAAACGG GCTCCAGTAA AACCCTCTCG 
GCTGCTGCAC GCAGATCCTT CTTCCGCAGG AAACACAAGC ACAAACGCAG CAGCTCCAAA 
GACGGGAAAG ACGTAGTGGC TTTGGACGCC ATCAGCACAG ACTCAATTCC TTTCTTAGAC 
GACTGTGTGA GTCTGGCTTA TCAGAGAGTG CAGAAGATTG ACTGCACATC TCCCAGACCT 
GTGCTGATCC TCGGCCCGCT TGTGGATCCT GTTAAAGACA TGCTGGTCAA AGATTCTCCA 
GGGAAATTCA GCAGATGTGT TCTTGAGGTG ATGAAGGCTT CTCAGCAGGC CATTGAGCGT 
GGGGTCAAAG ACTTTCTCTT CATCGACTAC AAACGAAGGA GTGGCCATTT TGATGTGACG 
ACTGTTGCCT CAATCAATGA AATCACGGAC AAGGACTGTC ACTGTTTGCT TGACATTGCA 
CCTCATGCTA TTGAAAGGCT TCACAGCGTT CACATTTACC CAATCGTCAT TTTCATACGC 
TACAAAAACG CAAAGCAGAT AAAAGAGCAG AAGGACCCGA TTTATCTGCG GGATAAAGTC 
TCTCAAAAAC ATTCCAAGGA GCAGTTTGAG ATTGCACAGA AGATTGAGCA AGACTACAGC 
AAGTTCTTCA CAGGTATCGT TCAGGGAGGC ACCTTCCCTT ACATTTGCAC TCAGATCGTG 
ACTATTGTGG ATCAGGAACA GAGCAAAGTC TTGTGGACTC CACTGGGCTC TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156739.1
CDS	997..6873
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKFFPQSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_005156682.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156682.3

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG CTTCATCACC TCCACCTGCG CTCAGTGACA CCCGGCCGGC CAGTGACAGT 
CTGGACACCA TCCTCTTCCA GCTGAAGCAG GTCACCAGGG AAAGGGACGA ACTTCGCAAG 
CGCTTGGCTC TCGCCTCTCC TGGCACCACC TTTGATGACT GCCGGCCAAA TTCCAAACCT 
GGTCATGACT ACGAGCGTCT GAAAACGCAG TGCACGAAGG CTATGGCGGA CCTGCAGTCT 
TTGCAGAACC AGCACACCAA AACTCTGAAG AGGTGCGAGG AAGCCGTGAA AGAAGCAGAC 
TATTACCACA TACAGCACAG CCGGCTCCTA AGTGAACAGA CACAGTTAAA AGAGGAGATG 
GAGGTTTTGA AAAGAGAGAA CACCAAGCTG ACTCGAGAAC ATAACCATCT GAAACAGAGC 
TGTGAGGAGC TGAGGAGGCT TCATTCTGAG GATCAGAGGG AGGTGGCTGA CATGAGACTA 
CAGCAACAAC AGGTAATCAG GGAAAACGGT TCATCTGAGG TTTTGAACAA GCTCTATGAC 
ACAGCGATGG ACAAGCTGGA GGGGGTGAAG AAGGAATATG ACGTCTTGAG CAAACGGTAC 
AGCGAGAAGG TGTCCAGTCA CAATACAGAT CTGAGTCGAC TGGAGCAGGT GGAGGAGGAG 
AACAGACGCT TGCAAAAACA AATCGACGCT CTGCTCAAAC AGCGCGATAC GGCCATCCAG 
TACCAACAGC AGTGGTCTAC ATCTATGAGG CGGTTTGACT CTGTGCAGCA GGAGCTAAAT 
AAGGCCTCGG CACAGAACAA GGAGCTGCAG AGAGAGATGG AGAGACTCCA GTCGGAGGTG 
ACGCGCTACA AGAACTTCCA GCTGAAGGCG GTGAAGGACG GTGAGAAGTA CAAGGAGGAG 
CGGGATTCTG TGTTTAACGA ATACCGGCTA ATCATGAGCG AAAGAGACCA GGTCATTAAA 
GAGGTGGACA AGCTGCAGAC GGAGTTGGAG GCTGCAGAGG CCCGGCTGAA AAACACCTCT 
TCTGAGAGGA TGGTGGCCAG CGAAGAGCTT GAAGCTCTCA GACAGGAGCT AAATTCATCG 
CTGGTGGATA GAGACCGTGC CATCTGTGAG AGAAACGAGC TGTTGGAAAA ATACTGCCAC 
GAAGTGAAGG ACAAAGCAGA GGCCCAGAAA GAGCTGAGTC AGGCCTGTAA AGACATCGAG 
ACCGTGCGGG AGGAAAGAGA CGTTGCCAGG AAAGAGCGAA CCGAAGCCAT CATCCAGAGA 
GACCAGTTAT TGCGTGAATA CTACCAGGCC CGACAGAAAC AAGACTCTGC CACATTGGAC 
ATGGAGCGTG CTAATAAAGA GATTGACATA TTGAGGAAGC AGTGTGAAGC CATGTCTCAG 
GAGCTGAAGG AGGCAGTGCA GGAGGCTGAG GTGGCTAAGT GTCGCAGAGA TTGGGCCTTT 
CAAGAGAGAG ACAAGATTGT TGCAGAGAGA GAGAGCATAC GGACGTTGTG TGATAACCTG 
CGTAGAGAGC GAGACCGAGC CGTCAGTGAT CTGGCCGATG CCCTACGCAA CCTTGATGAT 
ATGAGGAAAC AGCGCAATGA TGCGGCCAGG GAACTTAAAG AGCTCAAGGA GAAGATGGAG 
AGCCAGTTGG ACAAGGAGGC GCGTTTCTGC CAGCTGATGG CTCACAGCTC CCATGATTCA 
GCCATAGACA CTGATTCATT AGAGTGGGAG ACTGAAGTGG TGGAGTTTGA GAAGGATCGG 
GACGACATGG ATTTGAAGGC AATGGGGTTT GATATCGCAG AGGGGGTGAA TGATCCATAT 
TTACCAGGAG ACTGTGGGAT ATTTGTTTCT CGGGTGGACA AAGGAAGTAT CGCGGATGGA 
AGATTGAGGG TGAATGATTG GCTGCTGAAG ATAAATGATG TGGACCTGAC GAACAAAGAC 
AGGAAGCAGG TCATCAAAGC TGTACTTAAT GGAGGAGGCC TGATCAATAT GGTCGTTCGC 
AGGAGGAAAT CTTTAGGAGG AAGGCTGGTT ACTTCTGTTC ACATTAATCT CATCGGACAC 
AAAGACGCTG GTATAGCTCT TGAAGGTGGT GTATTCGTGG CTGGCATCGC ACCGGGCAGT 
CCAGCTGCCA GAGAAGGATC TCTTACCATC GGAGACCGCC TGATTGCCAT AAATGGGATC 
GCTCTGGATA ACAAATCTCT GAGTGAATGT GAGGCTCTGC TGAGGGGTGG CAGGGACTCG 
CTCGCTCTCT CTCTCATGAA GTTCTTTCCC CAGAGCACAT CAGGACAGAA CATCTTCGAG 
AGCCTGAGGG AGGCAGAGAA ATCCAATGGC AGAATCCACC TGACAGATGT GCACTCACGA 
AACAGCAGAA ACCTCAAACA CAACAGCTCC ACCCAGACAG ACATTTACAG CTGTGACACG 
GCCGGAGAGA GACGAAAAAC CAGATCTGAT GCAGAGGAGA GCCTTTTTTC TGAAAGGAAG 
GTCTTCCCCA TATCCACCTT GCACCCAAAC ACACTGCGAC CTGCTTCAGA ACTGGGACCA 
GTCCGTTACA GCGCCAGCGC CTTTCAGGAG GTCTGCTACA CTCGCTCAGA GTCCGTCGGC 
CCTGAATGTG TAACGTTGGA GACCACCTTA GAGAAGAAAC ATAGTGGCGG CACTTGGCCC 
AAAATGGTGG TTGGTGTCGC GATGACCACG GACAGCCCAG CGCAGCTTTC CATCTACAGG 
TCACCCAAAC AAAGAAAGTC CATCTTTGAT GCCGATACCT TCAAACGACC TGATACCCCC 
TCAAAAGCTG ATTACCATGG CCCTCAGCCT TTAAAAGCAG ACTCCGCCCC CACACCCCCA 
ACCCCGCCTA CTCGCAGCGA CTCCTTTAAA TTCAAGCACA AACATCAGGG AAGCTCCACC 
TCCGAGTCCA CCATTAGTGC CATGTCCATC TCTGAACCTA AGCAGGAAGA TCGTAATGGG 
AACTTGTACT TCAGTGAGGG TAAAGTCTTG AGCTCCAGGA AGTCCTGTGA GGAGGACATC 
GGTCGGATGT GGGCGGAGGA GCCCGAAGTG AAGCGTCCCA GGCCCAAATC GGCCCCGGCC 
CTCAGACGCA GGATGACTCC TCAAAACATT CCTCTCCAGA GCTTCCAGAG TTACTCTAAT 
GACGGTGACT CCGTGGAGCA GAGGGAGATG CTGCGCTCCT CTCCTAACCG ACCTCACAGA 
CACAGCGTGG GTTTCGTGCC TACGTCCTAT AATGGAACCC TTCCTCCCAA CTCAGCACAT 
CGAGGACTGG CCCCCTGCTC TGCAGTAACA GCCGTCATGA AGAACCCTGT TTACATGGTG 
CGCAGTCACA GAGTTCACAC TAACAACTGC CCTACTGTCG TCAGCCAGAT CAATCAGCAG 
CACACGCACC CCAGTCCTCA GCATCAAGGA CAACTGAGTC TGGACCTAAG CCAGAAACGC 
TCCACGGACT ATTCTGAGTC GTCCCGTAGC AGCCGGGCCT CACACGGCAC CAACTCCCTG 
CCGTCCAGTG CAAGACTCGG TTCATCAAAC AACTTGCAGT ATCGCACTGA AAGGATTAAG 
ATTCCCTCCA CACCGCGATA TCCTCGCTCT ATTCTGGGGT CAGACAGAGG GTCCCTGTCT 
CATTCAGAAT GCAGCAGTCC CAGTCTCATT ACTCCCCCTC TTTCTCCTCT CAATCTGGAG 
ACGTCTTCGT TTGCCTCCAG CCAGTCTCAG GGCTCCATCT CCACCCTGCC CAGGATCTCC 
GTCAGCCCCG TACCCACAGA AGAGCGCAGA AAAGACAGGC CGTACTTGGA GGAGCCACGG 
AATGTTATGG TGCACAAAGG GGCGGAGCCT CTCGGCATCT CCATTGTGAG TGGAGAGAAC 
GGAGGAATCT TTGTCTCCAA GGTGACAGGA GGCAGTATCG CGCACCAGGC TGGGTTGGAG 
TATGGGGACC AGCTATTGGA GTATAATGGA ATAAACCTGC GCAATGCCAC TGAACAGCAA 
GCACGACTCA TCATTGGCCA GCAGTGTGAC ACCATCACGA TCATGGCCCA ATACAACCCA 
CACATGTATC AGCTCGGCAA CCACTCGCGC TCCAGTTCCC GCCTGGAGCC AGTGAGCACT 
CAATCCACGC CACAGGGCAG TGGAACTGCA ACCCCAGATA ACCACTCTAC CATAGACACC 
CTGAGTGAAC AGGATGAAGG CACACTCACC CCATCCTCCA AACAGACCAC ACCCACAACC 
AGTCCCAACA GCTTCATCAG AATGCCGTCT GAAAGTTCCA AGAAAGTACC AGAGGCGCGT 
TTGGTGACAG TGAGGAAGAC TCAGGTGGAG CTGGGCATTC AGCTGTGTGG AGGAAACCTG 
CGGGGAATCT TCGTGGAGAG GCTGGAAGAT GACAGTCCTG CCAGAGGAGC GGATGCTGTT 
GTACCCGGGG ACATGATACT TGAGTATGGC TCAGTTAATA TGAAGAATAA GACTGTGGAG 
GAGGCATATC TGGAAATGCT GAAGCCTGCA GAAACAGTGA CGTTAAGAGT CCAGCATCGT 
CTGGATGAGT TCAACCAGGT GAAAGGCACT CCTGGAGATG GATTCTATAT CAGAGCACTT 
TATGACCGAG TAGCTGAGAC TGAGCTCGAC CTCTCTTTTA AGAAAGACGA CATCCTCTAT 
GTGGACGACA CACTACCAAA TGGCAACTTC GGCACCTGGA TGGCCTGGCA ACTTGACGAA 
AATGCACAAA AAATTCAAAG AGGCCAGATT CCTAGCAAAT TTATGATGGA TCAGGAGTTT 
TACCGTAGAC ACAGTATGAC TGAACTGAAG GACGAAACGG GCTCCAGTAA AACCCTCTCG 
GCTGCTGCAC GCAGATCCTT CTTCCGCAGG AAACACAAGC ACAAACGCAG CAGCTCCAAA 
GACGGGAAAG ACGTAGTGGC TTTGGACGCC ATCAGCACAG ACTCAATTCC TTTCTTAGAC 
GACTGTGTGA GTCTGGCTTA TCAGAGAGTG CAGAAGATTG ACTGCACATC TCCCAGACCT 
GTGCTGATCC TCGGCCCGCT TGTGGATCCT GTTAAAGACA TGCTGGTCAA AGATTCTCCA 
GGGAAATTCA GCAGATGTGT TCTTGAGGTG ATGAAGGCTT CTCAGCAGGC CATTGAGCGT 
GGGGTCAAAG ACTTTCTCTT CATCGACTAC AAACGAAGGA GTGGCCATTT TGATGTGACG 
ACTGTTGCCT CAATCAATGA AATCACGGAC AAGGACTGTC ACTGTTTGCT TGACATTGCA 
CCTCATGCTA TTGAAAGGCT TCACAGCGTT CACATTTACC CAATCGTCAT TTTCATACGC 
TACAAAAACG CAAAGCAGAT AAAAGAGCAG AAGGACCCGA TTTATCTGCG GGATAAAGTC 
TCTCAAAAAC ATTCCAAGGA GCAGTTTGAG ATTGCACAGA AGATTGAGCA AGACTACAGC 
AAGTTCTTCA CAGGTATCGT TCAGGGAGGC ACCTTCCCTT ACATTTGCAC TCAGATCGTG 
ACTATTGTGG ATCAGGAACA GAGCAAAGTC TTGTGGACTC CACTGGGCTC TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_009307256.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305531.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.

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CloneID	ODa45645
Clone ID Related Accession (Same CDS sequence)	XM_009307256.2 , XM_009307256.3
Accession Version	XM_009307256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disks large homolog 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG AGCACATCAG GACAGAACAT CTTCGAGAGC 
CTGAGGGAGG CAGAGAAATC CAATGGCAGA ATCCACCTGA CAGATGTGCA CTCACGAAAC 
AGCAGAAACC TCAAACACAA CAGCTCCACC CAGACAGACA TTTACAGCTG TGACACGGCC 
GGAGAGAGAC GAAAAACCAG ATCTGATGCA GAGGAGAGCC TTTTTTCTGA AAGGAAGGTC 
TTCCCCATAT CCACCTTGCA CCCAAACACA CTGCGACCTG CTTCAGAACT GGGACCAGTC 
CGTTACAGCG CCAGCGCCTT TCAGGAGGTC TGCTACACTC GCTCAGAGTC CGTCGGCCCT 
GAATGTGTAA CGTTGGAGAC CACCTTAGAG AAGAAACATA GTGGCGGCAC TTGGCCCAAA 
ATGGTGGTTG GTGTCGCGAT GACCACGGAC AGCCCAGCGC AGCTTTCCAT CTACAGGTCA 
CCCAAACAAA GAAAGTCCAT CTTTGATGCC GATACCTTCA AACGACCTGA TACCCCCTCA 
AAAGCTGATT ACCATGGCCC TCAGCCTTTA AAAGCAGACT CCGCCCCCAC ACCCCCAACC 
CCGCCTACTC GCAGCGACTC CTTTAAATTC AAGCACAAAC ATCAGGGAAG CTCCACCTCC 
GAGTCCACCA TTAGTGCCAT GTCCATCTCT GAACCTAAGC AGGAAGATCG TAATGGGAAC 
TTGTACTTCA GTGAGGGTAA AGTCTTGAGC TCCAGGAAGT CCTGTGAGGA GGACATCGGT 
CGGATGTGGG CGGAGGAGCC CGAAGTGAAG CGTCCCAGGC CCAAATCGGC CCCGGCCCTC 
AGACGCAGGA TGACTCCTCA AAACATTCCT CTCCAGAGCT TCCAGAGTTA CTCTAATGAC 
GGTGACTCCG TGGAGCAGAG GGAGATGCTG CGCTCCTCTC CTAACCGACC TCACAGACAC 
AGCGTGGGTT TCGTGCCTAC GTCCTATAAT GGAACCCTTC CTCCCAACTC AGCACATCGA 
GGACTGGCCC CCTGCTCTGC AGTAACAGCC GTCATGAAGA ACCCTGTTTA CATGGTGCGC 
AGTCACAGAG TTCACACTAA CAACTGCCCT ACTGTCGTCA GCCAGATCAA TCAGCAGCAC 
ACGCACCCCA GTCCTCAGCA TCAAGGACAA CTGAGTCTGG ACCTAAGCCA GAAACGCTCC 
ACGGACTATT CTGAGTCGTC CCGTAGCAGC CGGGCCTCAC ACGGCACCAA CTCCCTGCCG 
TCCAGTGCAA GACTCGGTTC ATCAAACAAC TTGCAGTATC GCACTGAAAG GATTAAGATT 
CCCTCCACAC CGCGATATCC TCGCTCTATT CTGGGGTCAG ACAGAGGGTC CCTGTCTCAT 
TCAGAATGCA GCAGTCCCAG TCTCATTACT CCCCCTCTTT CTCCTCTCAA TCTGGAGACG 
TCTTCGTTTG CCTCCAGCCA GTCTCAGGGC TCCATCTCCA CCCTGCCCAG GATCTCCGTC 
AGCCCCGTAC CCACAGAAGA GCGCAGAAAA GACAGGCCGT ACTTGGAGGA GCCACGGAAT 
GTTATGGTGC ACAAAGGGGC GGAGCCTCTC GGCATCTCCA TTGTGAGTGG AGAGAACGGA 
GGAATCTTTG TCTCCAAGGT GACAGGAGGC AGTATCGCGC ACCAGGCTGG GTTGGAGTAT 
GGGGACCAGC TATTGGAGTA TAATGGAATA AACCTGCGCA ATGCCACTGA ACAGCAAGCA 
CGACTCATCA TTGGCCAGCA GTGTGACACC ATCACGATCA TGGCCCAATA CAACCCACAC 
ATGTATCAGC TCGGCAACCA CTCGCGCTCC AGTTCCCGCC TGGAGCCAGT GAGCACTCAA 
TCCACGCCAC AGGGCAGTGG AACTGCAACC CCAGATAACC ACTCTACCAT AGACACCCTG 
AGTGAACAGG ATGAAGGCAC ACTCACCCCA TCCTCCAAAC AGACCACACC CACAACCAGT 
CCCAACAGCT TCATCAGAAT GCCGTCTGAA AGTTCCAAGA AAGTACCAGA GGCGCGTTTG 
GTGACAGTGA GGAAGACTCA GGTGGAGCTG GGCATTCAGC TGTGTGGAGG AAACCTGCGG 
GGAATCTTCG TGGAGAGGCT GGAAGATGAC AGTCCTGCCA GAGGAGCGGA TGCTGTTGTA 
CCCGGGGACA TGATACTTGA GTATGGCTCA GTTAATATGA AGAATAAGAC TGTGGAGGAG 
GCATATCTGG AAATGCTGAA GCCTGCAGAA ACAGTGACGT TAAGAGTCCA GCATCGTCTG 
GATGAGTTCA ACCAGGTGAA AGGCACTCCT GGAGATGGAT TCTATATCAG AGCACTTTAT 
GACCGAGTAG CTGAGACTGA GCTCGACCTC TCTTTTAAGA AAGACGACAT CCTCTATGTG 
GACGACACAC TACCAAATGG CAACTTCGGC ACCTGGATGG CCTGGCAACT TGACGAAAAT 
GCACAAAAAA TTCAAAGAGG CCAGATTCCT AGCAAATTTA TGATGGATCA GGAGTTTTAC 
CGTAGACACA GTATGACTGA ACTGAAGGAC GAAACGGGCT CCAGTAAAAC CCTCTCGGCT 
GCTGCACGCA GATCCTTCTT CCGCAGGAAA CACAAGCACA AACGCAGCAG CTCCAAAGAC 
GGGAAAGACG TAGTGGCTTT GGACGCCATC AGCACAGACT CAATTCCTTT CTTAGACGAC 
TGTGTGAGTC TGGCTTATCA GAGAGTGCAG AAGATTGACT GCACATCTCC CAGACCTGTG 
CTGATCCTCG GCCCGCTTGT GGATCCTGTT AAAGACATGC TGGTCAAAGA TTCTCCAGGG 
AAATTCAGCA GATGTGTTCT TGAGGTGATG AAGGCTTCTC AGCAGGCCAT TGAGCGTGGG 
GTCAAAGACT TTCTCTTCAT CGACTACAAA CGAAGGAGTG GCCATTTTGA TGTGACGACT 
GTTGCCTCAA TCAATGAAAT CACGGACAAG GACTGTCACT GTTTGCTTGA CATTGCACCT 
CATGCTATTG AAAGGCTTCA CAGCGTTCAC ATTTACCCAA TCGTCATTTT CATACGCTAC 
AAAAACGCAA AGCAGATAAA AGAGCAGAAG GACCCGATTT ATCTGCGGGA TAAAGTCTCT 
CAAAAACATT CCAAGGAGCA GTTTGAGATT GCACAGAAGA TTGAGCAAGA CTACAGCAAG 
TTCTTCACAG GTATCGTTCA GGGAGGCACC TTCCCTTACA TTTGCACTCA GATCGTGACT 
ATTGTGGATC AGGAACAGAG CAAAGTCTTG TGGACTCCAC TGGGCTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305531.1
CDS	997..6870
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_009307256.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307256.2

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG AGCACATCAG GACAGAACAT CTTCGAGAGC 
CTGAGGGAGG CAGAGAAATC CAATGGCAGA ATCCACCTGA CAGATGTGCA CTCACGAAAC 
AGCAGAAACC TCAAACACAA CAGCTCCACC CAGACAGACA TTTACAGCTG TGACACGGCC 
GGAGAGAGAC GAAAAACCAG ATCTGATGCA GAGGAGAGCC TTTTTTCTGA AAGGAAGGTC 
TTCCCCATAT CCACCTTGCA CCCAAACACA CTGCGACCTG CTTCAGAACT GGGACCAGTC 
CGTTACAGCG CCAGCGCCTT TCAGGAGGTC TGCTACACTC GCTCAGAGTC CGTCGGCCCT 
GAATGTGTAA CGTTGGAGAC CACCTTAGAG AAGAAACATA GTGGCGGCAC TTGGCCCAAA 
ATGGTGGTTG GTGTCGCGAT GACCACGGAC AGCCCAGCGC AGCTTTCCAT CTACAGGTCA 
CCCAAACAAA GAAAGTCCAT CTTTGATGCC GATACCTTCA AACGACCTGA TACCCCCTCA 
AAAGCTGATT ACCATGGCCC TCAGCCTTTA AAAGCAGACT CCGCCCCCAC ACCCCCAACC 
CCGCCTACTC GCAGCGACTC CTTTAAATTC AAGCACAAAC ATCAGGGAAG CTCCACCTCC 
GAGTCCACCA TTAGTGCCAT GTCCATCTCT GAACCTAAGC AGGAAGATCG TAATGGGAAC 
TTGTACTTCA GTGAGGGTAA AGTCTTGAGC TCCAGGAAGT CCTGTGAGGA GGACATCGGT 
CGGATGTGGG CGGAGGAGCC CGAAGTGAAG CGTCCCAGGC CCAAATCGGC CCCGGCCCTC 
AGACGCAGGA TGACTCCTCA AAACATTCCT CTCCAGAGCT TCCAGAGTTA CTCTAATGAC 
GGTGACTCCG TGGAGCAGAG GGAGATGCTG CGCTCCTCTC CTAACCGACC TCACAGACAC 
AGCGTGGGTT TCGTGCCTAC GTCCTATAAT GGAACCCTTC CTCCCAACTC AGCACATCGA 
GGACTGGCCC CCTGCTCTGC AGTAACAGCC GTCATGAAGA ACCCTGTTTA CATGGTGCGC 
AGTCACAGAG TTCACACTAA CAACTGCCCT ACTGTCGTCA GCCAGATCAA TCAGCAGCAC 
ACGCACCCCA GTCCTCAGCA TCAAGGACAA CTGAGTCTGG ACCTAAGCCA GAAACGCTCC 
ACGGACTATT CTGAGTCGTC CCGTAGCAGC CGGGCCTCAC ACGGCACCAA CTCCCTGCCG 
TCCAGTGCAA GACTCGGTTC ATCAAACAAC TTGCAGTATC GCACTGAAAG GATTAAGATT 
CCCTCCACAC CGCGATATCC TCGCTCTATT CTGGGGTCAG ACAGAGGGTC CCTGTCTCAT 
TCAGAATGCA GCAGTCCCAG TCTCATTACT CCCCCTCTTT CTCCTCTCAA TCTGGAGACG 
TCTTCGTTTG CCTCCAGCCA GTCTCAGGGC TCCATCTCCA CCCTGCCCAG GATCTCCGTC 
AGCCCCGTAC CCACAGAAGA GCGCAGAAAA GACAGGCCGT ACTTGGAGGA GCCACGGAAT 
GTTATGGTGC ACAAAGGGGC GGAGCCTCTC GGCATCTCCA TTGTGAGTGG AGAGAACGGA 
GGAATCTTTG TCTCCAAGGT GACAGGAGGC AGTATCGCGC ACCAGGCTGG GTTGGAGTAT 
GGGGACCAGC TATTGGAGTA TAATGGAATA AACCTGCGCA ATGCCACTGA ACAGCAAGCA 
CGACTCATCA TTGGCCAGCA GTGTGACACC ATCACGATCA TGGCCCAATA CAACCCACAC 
ATGTATCAGC TCGGCAACCA CTCGCGCTCC AGTTCCCGCC TGGAGCCAGT GAGCACTCAA 
TCCACGCCAC AGGGCAGTGG AACTGCAACC CCAGATAACC ACTCTACCAT AGACACCCTG 
AGTGAACAGG ATGAAGGCAC ACTCACCCCA TCCTCCAAAC AGACCACACC CACAACCAGT 
CCCAACAGCT TCATCAGAAT GCCGTCTGAA AGTTCCAAGA AAGTACCAGA GGCGCGTTTG 
GTGACAGTGA GGAAGACTCA GGTGGAGCTG GGCATTCAGC TGTGTGGAGG AAACCTGCGG 
GGAATCTTCG TGGAGAGGCT GGAAGATGAC AGTCCTGCCA GAGGAGCGGA TGCTGTTGTA 
CCCGGGGACA TGATACTTGA GTATGGCTCA GTTAATATGA AGAATAAGAC TGTGGAGGAG 
GCATATCTGG AAATGCTGAA GCCTGCAGAA ACAGTGACGT TAAGAGTCCA GCATCGTCTG 
GATGAGTTCA ACCAGGTGAA AGGCACTCCT GGAGATGGAT TCTATATCAG AGCACTTTAT 
GACCGAGTAG CTGAGACTGA GCTCGACCTC TCTTTTAAGA AAGACGACAT CCTCTATGTG 
GACGACACAC TACCAAATGG CAACTTCGGC ACCTGGATGG CCTGGCAACT TGACGAAAAT 
GCACAAAAAA TTCAAAGAGG CCAGATTCCT AGCAAATTTA TGATGGATCA GGAGTTTTAC 
CGTAGACACA GTATGACTGA ACTGAAGGAC GAAACGGGCT CCAGTAAAAC CCTCTCGGCT 
GCTGCACGCA GATCCTTCTT CCGCAGGAAA CACAAGCACA AACGCAGCAG CTCCAAAGAC 
GGGAAAGACG TAGTGGCTTT GGACGCCATC AGCACAGACT CAATTCCTTT CTTAGACGAC 
TGTGTGAGTC TGGCTTATCA GAGAGTGCAG AAGATTGACT GCACATCTCC CAGACCTGTG 
CTGATCCTCG GCCCGCTTGT GGATCCTGTT AAAGACATGC TGGTCAAAGA TTCTCCAGGG 
AAATTCAGCA GATGTGTTCT TGAGGTGATG AAGGCTTCTC AGCAGGCCAT TGAGCGTGGG 
GTCAAAGACT TTCTCTTCAT CGACTACAAA CGAAGGAGTG GCCATTTTGA TGTGACGACT 
GTTGCCTCAA TCAATGAAAT CACGGACAAG GACTGTCACT GTTTGCTTGA CATTGCACCT 
CATGCTATTG AAAGGCTTCA CAGCGTTCAC ATTTACCCAA TCGTCATTTT CATACGCTAC 
AAAAACGCAA AGCAGATAAA AGAGCAGAAG GACCCGATTT ATCTGCGGGA TAAAGTCTCT 
CAAAAACATT CCAAGGAGCA GTTTGAGATT GCACAGAAGA TTGAGCAAGA CTACAGCAAG 
TTCTTCACAG GTATCGTTCA GGGAGGCACC TTCCCTTACA TTTGCACTCA GATCGTGACT 
ATTGTGGATC AGGAACAGAG CAAAGTCTTG TGGACTCCAC TGGGCTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlg5a ( XM_009307256.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305531.1 Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.

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CloneID	ODa45645
Clone ID Related Accession (Same CDS sequence)	XM_009307256.2 , XM_009307256.3
Accession Version	XM_009307256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disks large homolog 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG AGCACATCAG GACAGAACAT CTTCGAGAGC 
CTGAGGGAGG CAGAGAAATC CAATGGCAGA ATCCACCTGA CAGATGTGCA CTCACGAAAC 
AGCAGAAACC TCAAACACAA CAGCTCCACC CAGACAGACA TTTACAGCTG TGACACGGCC 
GGAGAGAGAC GAAAAACCAG ATCTGATGCA GAGGAGAGCC TTTTTTCTGA AAGGAAGGTC 
TTCCCCATAT CCACCTTGCA CCCAAACACA CTGCGACCTG CTTCAGAACT GGGACCAGTC 
CGTTACAGCG CCAGCGCCTT TCAGGAGGTC TGCTACACTC GCTCAGAGTC CGTCGGCCCT 
GAATGTGTAA CGTTGGAGAC CACCTTAGAG AAGAAACATA GTGGCGGCAC TTGGCCCAAA 
ATGGTGGTTG GTGTCGCGAT GACCACGGAC AGCCCAGCGC AGCTTTCCAT CTACAGGTCA 
CCCAAACAAA GAAAGTCCAT CTTTGATGCC GATACCTTCA AACGACCTGA TACCCCCTCA 
AAAGCTGATT ACCATGGCCC TCAGCCTTTA AAAGCAGACT CCGCCCCCAC ACCCCCAACC 
CCGCCTACTC GCAGCGACTC CTTTAAATTC AAGCACAAAC ATCAGGGAAG CTCCACCTCC 
GAGTCCACCA TTAGTGCCAT GTCCATCTCT GAACCTAAGC AGGAAGATCG TAATGGGAAC 
TTGTACTTCA GTGAGGGTAA AGTCTTGAGC TCCAGGAAGT CCTGTGAGGA GGACATCGGT 
CGGATGTGGG CGGAGGAGCC CGAAGTGAAG CGTCCCAGGC CCAAATCGGC CCCGGCCCTC 
AGACGCAGGA TGACTCCTCA AAACATTCCT CTCCAGAGCT TCCAGAGTTA CTCTAATGAC 
GGTGACTCCG TGGAGCAGAG GGAGATGCTG CGCTCCTCTC CTAACCGACC TCACAGACAC 
AGCGTGGGTT TCGTGCCTAC GTCCTATAAT GGAACCCTTC CTCCCAACTC AGCACATCGA 
GGACTGGCCC CCTGCTCTGC AGTAACAGCC GTCATGAAGA ACCCTGTTTA CATGGTGCGC 
AGTCACAGAG TTCACACTAA CAACTGCCCT ACTGTCGTCA GCCAGATCAA TCAGCAGCAC 
ACGCACCCCA GTCCTCAGCA TCAAGGACAA CTGAGTCTGG ACCTAAGCCA GAAACGCTCC 
ACGGACTATT CTGAGTCGTC CCGTAGCAGC CGGGCCTCAC ACGGCACCAA CTCCCTGCCG 
TCCAGTGCAA GACTCGGTTC ATCAAACAAC TTGCAGTATC GCACTGAAAG GATTAAGATT 
CCCTCCACAC CGCGATATCC TCGCTCTATT CTGGGGTCAG ACAGAGGGTC CCTGTCTCAT 
TCAGAATGCA GCAGTCCCAG TCTCATTACT CCCCCTCTTT CTCCTCTCAA TCTGGAGACG 
TCTTCGTTTG CCTCCAGCCA GTCTCAGGGC TCCATCTCCA CCCTGCCCAG GATCTCCGTC 
AGCCCCGTAC CCACAGAAGA GCGCAGAAAA GACAGGCCGT ACTTGGAGGA GCCACGGAAT 
GTTATGGTGC ACAAAGGGGC GGAGCCTCTC GGCATCTCCA TTGTGAGTGG AGAGAACGGA 
GGAATCTTTG TCTCCAAGGT GACAGGAGGC AGTATCGCGC ACCAGGCTGG GTTGGAGTAT 
GGGGACCAGC TATTGGAGTA TAATGGAATA AACCTGCGCA ATGCCACTGA ACAGCAAGCA 
CGACTCATCA TTGGCCAGCA GTGTGACACC ATCACGATCA TGGCCCAATA CAACCCACAC 
ATGTATCAGC TCGGCAACCA CTCGCGCTCC AGTTCCCGCC TGGAGCCAGT GAGCACTCAA 
TCCACGCCAC AGGGCAGTGG AACTGCAACC CCAGATAACC ACTCTACCAT AGACACCCTG 
AGTGAACAGG ATGAAGGCAC ACTCACCCCA TCCTCCAAAC AGACCACACC CACAACCAGT 
CCCAACAGCT TCATCAGAAT GCCGTCTGAA AGTTCCAAGA AAGTACCAGA GGCGCGTTTG 
GTGACAGTGA GGAAGACTCA GGTGGAGCTG GGCATTCAGC TGTGTGGAGG AAACCTGCGG 
GGAATCTTCG TGGAGAGGCT GGAAGATGAC AGTCCTGCCA GAGGAGCGGA TGCTGTTGTA 
CCCGGGGACA TGATACTTGA GTATGGCTCA GTTAATATGA AGAATAAGAC TGTGGAGGAG 
GCATATCTGG AAATGCTGAA GCCTGCAGAA ACAGTGACGT TAAGAGTCCA GCATCGTCTG 
GATGAGTTCA ACCAGGTGAA AGGCACTCCT GGAGATGGAT TCTATATCAG AGCACTTTAT 
GACCGAGTAG CTGAGACTGA GCTCGACCTC TCTTTTAAGA AAGACGACAT CCTCTATGTG 
GACGACACAC TACCAAATGG CAACTTCGGC ACCTGGATGG CCTGGCAACT TGACGAAAAT 
GCACAAAAAA TTCAAAGAGG CCAGATTCCT AGCAAATTTA TGATGGATCA GGAGTTTTAC 
CGTAGACACA GTATGACTGA ACTGAAGGAC GAAACGGGCT CCAGTAAAAC CCTCTCGGCT 
GCTGCACGCA GATCCTTCTT CCGCAGGAAA CACAAGCACA AACGCAGCAG CTCCAAAGAC 
GGGAAAGACG TAGTGGCTTT GGACGCCATC AGCACAGACT CAATTCCTTT CTTAGACGAC 
TGTGTGAGTC TGGCTTATCA GAGAGTGCAG AAGATTGACT GCACATCTCC CAGACCTGTG 
CTGATCCTCG GCCCGCTTGT GGATCCTGTT AAAGACATGC TGGTCAAAGA TTCTCCAGGG 
AAATTCAGCA GATGTGTTCT TGAGGTGATG AAGGCTTCTC AGCAGGCCAT TGAGCGTGGG 
GTCAAAGACT TTCTCTTCAT CGACTACAAA CGAAGGAGTG GCCATTTTGA TGTGACGACT 
GTTGCCTCAA TCAATGAAAT CACGGACAAG GACTGTCACT GTTTGCTTGA CATTGCACCT 
CATGCTATTG AAAGGCTTCA CAGCGTTCAC ATTTACCCAA TCGTCATTTT CATACGCTAC 
AAAAACGCAA AGCAGATAAA AGAGCAGAAG GACCCGATTT ATCTGCGGGA TAAAGTCTCT 
CAAAAACATT CCAAGGAGCA GTTTGAGATT GCACAGAAGA TTGAGCAAGA CTACAGCAAG 
TTCTTCACAG GTATCGTTCA GGGAGGCACC TTCCCTTACA TTTGCACTCA GATCGTGACT 
ATTGTGGATC AGGAACAGAG CAAAGTCTTG TGGACTCCAC TGGGCTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305531.1
CDS	977..6850
Translation	MEPKHKELLHQCHQNLLESITDADRLIELLSKSGSLSEQEAFELDQNCSSSAEKVEQLLKMLMNKQSDHFLELCVALEKTYPHLYSALFSNGGGPADHHSGSTYSVLSTMPSDSESSSSLSSVGTKASSPPPALSDTRPASDSLDTILFQLKQVTRERDELRKRLALASPGTTFDDCRPNSKPGHDYERLKTQCTKAMADLQSLQNQHTKTLKRCEEAVKEADYYHIQHSRLLSEQTQLKEEMEVLKRENTKLTREHNHLKQSCEELRRLHSEDQREVADMRLQQQQVIRENGSSEVLNKLYDTAMDKLEGVKKEYDVLSKRYSEKVSSHNTDLSRLEQVEEENRRLQKQIDALLKQRDTAIQYQQQWSTSMRRFDSVQQELNKASAQNKELQREMERLQSEVTRYKNFQLKAVKDGEKYKEERDSVFNEYRLIMSERDQVIKEVDKLQTELEAAEARLKNTSSERMVASEELEALRQELNSSLVDRDRAICERNELLEKYCHEVKDKAEAQKELSQACKDIETVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIDILRKQCEAMSQELKEAVQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDMRKQRNDAARELKELKEKMESQLDKEARFCQLMAHSSHDSAIDTDSLEWETEVVEFEKDRDDMDLKAMGFDIAEGVNDPYLPGDCGIFVSRVDKGSIADGRLRVNDWLLKINDVDLTNKDRKQVIKAVLNGGGLINMVVRRRKSLGGRLVTSVHINLIGHKDAGIALEGGVFVAGIAPGSPAAREGSLTIGDRLIAINGIALDNKSLSECEALLRGGRDSLALSLMKSTSGQNIFESLREAEKSNGRIHLTDVHSRNSRNLKHNSSTQTDIYSCDTAGERRKTRSDAEESLFSERKVFPISTLHPNTLRPASELGPVRYSASAFQEVCYTRSESVGPECVTLETTLEKKHSGGTWPKMVVGVAMTTDSPAQLSIYRSPKQRKSIFDADTFKRPDTPSKADYHGPQPLKADSAPTPPTPPTRSDSFKFKHKHQGSSTSESTISAMSISEPKQEDRNGNLYFSEGKVLSSRKSCEEDIGRMWAEEPEVKRPRPKSAPALRRRMTPQNIPLQSFQSYSNDGDSVEQREMLRSSPNRPHRHSVGFVPTSYNGTLPPNSAHRGLAPCSAVTAVMKNPVYMVRSHRVHTNNCPTVVSQINQQHTHPSPQHQGQLSLDLSQKRSTDYSESSRSSRASHGTNSLPSSARLGSSNNLQYRTERIKIPSTPRYPRSILGSDRGSLSHSECSSPSLITPPLSPLNLETSSFASSQSQGSISTLPRISVSPVPTEERRKDRPYLEEPRNVMVHKGAEPLGISIVSGENGGIFVSKVTGGSIAHQAGLEYGDQLLEYNGINLRNATEQQARLIIGQQCDTITIMAQYNPHMYQLGNHSRSSSRLEPVSTQSTPQGSGTATPDNHSTIDTLSEQDEGTLTPSSKQTTPTTSPNSFIRMPSESSKKVPEARLVTVRKTQVELGIQLCGGNLRGIFVERLEDDSPARGADAVVPGDMILEYGSVNMKNKTVEEAYLEMLKPAETVTLRVQHRLDEFNQVKGTPGDGFYIRALYDRVAETELDLSFKKDDILYVDDTLPNGNFGTWMAWQLDENAQKIQRGQIPSKFMMDQEFYRRHSMTELKDETGSSKTLSAAARRSFFRRKHKHKRSSSKDGKDVVALDAISTDSIPFLDDCVSLAYQRVQKIDCTSPRPVLILGPLVDPVKDMLVKDSPGKFSRCVLEVMKASQQAIERGVKDFLFIDYKRRSGHFDVTTVASINEITDKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPIYLRDKVSQKHSKEQFEIAQKIEQDYSKFFTGIVQGGTFPYICTQIVTIVDQEQSKVLWTPLGSP

Target ORF information:

RefSeq Version	XM_009307256.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio discs, large homolog 5a (Drosophila) (dlg5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307256.3

ORF Insert Sequence:

ATGGAGCCCA AGCACAAAGA GTTACTCCAC CAGTGCCACC AGAACTTGCT GGAGTCCATC 
ACGGATGCTG ACCGGCTGAT TGAGCTGCTG AGTAAATCGG GAAGCCTCAG CGAGCAGGAA 
GCGTTCGAGC TGGACCAGAA CTGCTCCTCC AGCGCAGAGA AAGTGGAGCA GCTCCTGAAG 
ATGCTCATGA ACAAGCAGAG CGACCATTTC CTGGAGCTCT GTGTGGCCCT GGAGAAGACA 
TACCCTCACC TGTACTCTGC CCTTTTCTCC AACGGTGGAG GACCAGCCGA TCATCACTCT 
GGGTCCACAT ACAGTGTCCT GTCCACAATG CCATCAGACT CGGAGAGCAG CAGCTCCCTA 
AGCAGTGTTG GTACCAAAGC TTCATCACCT CCACCTGCGC TCAGTGACAC CCGGCCGGCC 
AGTGACAGTC TGGACACCAT CCTCTTCCAG CTGAAGCAGG TCACCAGGGA AAGGGACGAA 
CTTCGCAAGC GCTTGGCTCT CGCCTCTCCT GGCACCACCT TTGATGACTG CCGGCCAAAT 
TCCAAACCTG GTCATGACTA CGAGCGTCTG AAAACGCAGT GCACGAAGGC TATGGCGGAC 
CTGCAGTCTT TGCAGAACCA GCACACCAAA ACTCTGAAGA GGTGCGAGGA AGCCGTGAAA 
GAAGCAGACT ATTACCACAT ACAGCACAGC CGGCTCCTAA GTGAACAGAC ACAGTTAAAA 
GAGGAGATGG AGGTTTTGAA AAGAGAGAAC ACCAAGCTGA CTCGAGAACA TAACCATCTG 
AAACAGAGCT GTGAGGAGCT GAGGAGGCTT CATTCTGAGG ATCAGAGGGA GGTGGCTGAC 
ATGAGACTAC AGCAACAACA GGTAATCAGG GAAAACGGTT CATCTGAGGT TTTGAACAAG 
CTCTATGACA CAGCGATGGA CAAGCTGGAG GGGGTGAAGA AGGAATATGA CGTCTTGAGC 
AAACGGTACA GCGAGAAGGT GTCCAGTCAC AATACAGATC TGAGTCGACT GGAGCAGGTG 
GAGGAGGAGA ACAGACGCTT GCAAAAACAA ATCGACGCTC TGCTCAAACA GCGCGATACG 
GCCATCCAGT ACCAACAGCA GTGGTCTACA TCTATGAGGC GGTTTGACTC TGTGCAGCAG 
GAGCTAAATA AGGCCTCGGC ACAGAACAAG GAGCTGCAGA GAGAGATGGA GAGACTCCAG 
TCGGAGGTGA CGCGCTACAA GAACTTCCAG CTGAAGGCGG TGAAGGACGG TGAGAAGTAC 
AAGGAGGAGC GGGATTCTGT GTTTAACGAA TACCGGCTAA TCATGAGCGA AAGAGACCAG 
GTCATTAAAG AGGTGGACAA GCTGCAGACG GAGTTGGAGG CTGCAGAGGC CCGGCTGAAA 
AACACCTCTT CTGAGAGGAT GGTGGCCAGC GAAGAGCTTG AAGCTCTCAG ACAGGAGCTA 
AATTCATCGC TGGTGGATAG AGACCGTGCC ATCTGTGAGA GAAACGAGCT GTTGGAAAAA 
TACTGCCACG AAGTGAAGGA CAAAGCAGAG GCCCAGAAAG AGCTGAGTCA GGCCTGTAAA 
GACATCGAGA CCGTGCGGGA GGAAAGAGAC GTTGCCAGGA AAGAGCGAAC CGAAGCCATC 
ATCCAGAGAG ACCAGTTATT GCGTGAATAC TACCAGGCCC GACAGAAACA AGACTCTGCC 
ACATTGGACA TGGAGCGTGC TAATAAAGAG ATTGACATAT TGAGGAAGCA GTGTGAAGCC 
ATGTCTCAGG AGCTGAAGGA GGCAGTGCAG GAGGCTGAGG TGGCTAAGTG TCGCAGAGAT 
TGGGCCTTTC AAGAGAGAGA CAAGATTGTT GCAGAGAGAG AGAGCATACG GACGTTGTGT 
GATAACCTGC GTAGAGAGCG AGACCGAGCC GTCAGTGATC TGGCCGATGC CCTACGCAAC 
CTTGATGATA TGAGGAAACA GCGCAATGAT GCGGCCAGGG AACTTAAAGA GCTCAAGGAG 
AAGATGGAGA GCCAGTTGGA CAAGGAGGCG CGTTTCTGCC AGCTGATGGC TCACAGCTCC 
CATGATTCAG CCATAGACAC TGATTCATTA GAGTGGGAGA CTGAAGTGGT GGAGTTTGAG 
AAGGATCGGG ACGACATGGA TTTGAAGGCA ATGGGGTTTG ATATCGCAGA GGGGGTGAAT 
GATCCATATT TACCAGGAGA CTGTGGGATA TTTGTTTCTC GGGTGGACAA AGGAAGTATC 
GCGGATGGAA GATTGAGGGT GAATGATTGG CTGCTGAAGA TAAATGATGT GGACCTGACG 
AACAAAGACA GGAAGCAGGT CATCAAAGCT GTACTTAATG GAGGAGGCCT GATCAATATG 
GTCGTTCGCA GGAGGAAATC TTTAGGAGGA AGGCTGGTTA CTTCTGTTCA CATTAATCTC 
ATCGGACACA AAGACGCTGG TATAGCTCTT GAAGGTGGTG TATTCGTGGC TGGCATCGCA 
CCGGGCAGTC CAGCTGCCAG AGAAGGATCT CTTACCATCG GAGACCGCCT GATTGCCATA 
AATGGGATCG CTCTGGATAA CAAATCTCTG AGTGAATGTG AGGCTCTGCT GAGGGGTGGC 
AGGGACTCGC TCGCTCTCTC TCTCATGAAG AGCACATCAG GACAGAACAT CTTCGAGAGC 
CTGAGGGAGG CAGAGAAATC CAATGGCAGA ATCCACCTGA CAGATGTGCA CTCACGAAAC 
AGCAGAAACC TCAAACACAA CAGCTCCACC CAGACAGACA TTTACAGCTG TGACACGGCC 
GGAGAGAGAC GAAAAACCAG ATCTGATGCA GAGGAGAGCC TTTTTTCTGA AAGGAAGGTC 
TTCCCCATAT CCACCTTGCA CCCAAACACA CTGCGACCTG CTTCAGAACT GGGACCAGTC 
CGTTACAGCG CCAGCGCCTT TCAGGAGGTC TGCTACACTC GCTCAGAGTC CGTCGGCCCT 
GAATGTGTAA CGTTGGAGAC CACCTTAGAG AAGAAACATA GTGGCGGCAC TTGGCCCAAA 
ATGGTGGTTG GTGTCGCGAT GACCACGGAC AGCCCAGCGC AGCTTTCCAT CTACAGGTCA 
CCCAAACAAA GAAAGTCCAT CTTTGATGCC GATACCTTCA AACGACCTGA TACCCCCTCA 
AAAGCTGATT ACCATGGCCC TCAGCCTTTA AAAGCAGACT CCGCCCCCAC ACCCCCAACC 
CCGCCTACTC GCAGCGACTC CTTTAAATTC AAGCACAAAC ATCAGGGAAG CTCCACCTCC 
GAGTCCACCA TTAGTGCCAT GTCCATCTCT GAACCTAAGC AGGAAGATCG TAATGGGAAC 
TTGTACTTCA GTGAGGGTAA AGTCTTGAGC TCCAGGAAGT CCTGTGAGGA GGACATCGGT 
CGGATGTGGG CGGAGGAGCC CGAAGTGAAG CGTCCCAGGC CCAAATCGGC CCCGGCCCTC 
AGACGCAGGA TGACTCCTCA AAACATTCCT CTCCAGAGCT TCCAGAGTTA CTCTAATGAC 
GGTGACTCCG TGGAGCAGAG GGAGATGCTG CGCTCCTCTC CTAACCGACC TCACAGACAC 
AGCGTGGGTT TCGTGCCTAC GTCCTATAAT GGAACCCTTC CTCCCAACTC AGCACATCGA 
GGACTGGCCC CCTGCTCTGC AGTAACAGCC GTCATGAAGA ACCCTGTTTA CATGGTGCGC 
AGTCACAGAG TTCACACTAA CAACTGCCCT ACTGTCGTCA GCCAGATCAA TCAGCAGCAC 
ACGCACCCCA GTCCTCAGCA TCAAGGACAA CTGAGTCTGG ACCTAAGCCA GAAACGCTCC 
ACGGACTATT CTGAGTCGTC CCGTAGCAGC CGGGCCTCAC ACGGCACCAA CTCCCTGCCG 
TCCAGTGCAA GACTCGGTTC ATCAAACAAC TTGCAGTATC GCACTGAAAG GATTAAGATT 
CCCTCCACAC CGCGATATCC TCGCTCTATT CTGGGGTCAG ACAGAGGGTC CCTGTCTCAT 
TCAGAATGCA GCAGTCCCAG TCTCATTACT CCCCCTCTTT CTCCTCTCAA TCTGGAGACG 
TCTTCGTTTG CCTCCAGCCA GTCTCAGGGC TCCATCTCCA CCCTGCCCAG GATCTCCGTC 
AGCCCCGTAC CCACAGAAGA GCGCAGAAAA GACAGGCCGT ACTTGGAGGA GCCACGGAAT 
GTTATGGTGC ACAAAGGGGC GGAGCCTCTC GGCATCTCCA TTGTGAGTGG AGAGAACGGA 
GGAATCTTTG TCTCCAAGGT GACAGGAGGC AGTATCGCGC ACCAGGCTGG GTTGGAGTAT 
GGGGACCAGC TATTGGAGTA TAATGGAATA AACCTGCGCA ATGCCACTGA ACAGCAAGCA 
CGACTCATCA TTGGCCAGCA GTGTGACACC ATCACGATCA TGGCCCAATA CAACCCACAC 
ATGTATCAGC TCGGCAACCA CTCGCGCTCC AGTTCCCGCC TGGAGCCAGT GAGCACTCAA 
TCCACGCCAC AGGGCAGTGG AACTGCAACC CCAGATAACC ACTCTACCAT AGACACCCTG 
AGTGAACAGG ATGAAGGCAC ACTCACCCCA TCCTCCAAAC AGACCACACC CACAACCAGT 
CCCAACAGCT TCATCAGAAT GCCGTCTGAA AGTTCCAAGA AAGTACCAGA GGCGCGTTTG 
GTGACAGTGA GGAAGACTCA GGTGGAGCTG GGCATTCAGC TGTGTGGAGG AAACCTGCGG 
GGAATCTTCG TGGAGAGGCT GGAAGATGAC AGTCCTGCCA GAGGAGCGGA TGCTGTTGTA 
CCCGGGGACA TGATACTTGA GTATGGCTCA GTTAATATGA AGAATAAGAC TGTGGAGGAG 
GCATATCTGG AAATGCTGAA GCCTGCAGAA ACAGTGACGT TAAGAGTCCA GCATCGTCTG 
GATGAGTTCA ACCAGGTGAA AGGCACTCCT GGAGATGGAT TCTATATCAG AGCACTTTAT 
GACCGAGTAG CTGAGACTGA GCTCGACCTC TCTTTTAAGA AAGACGACAT CCTCTATGTG 
GACGACACAC TACCAAATGG CAACTTCGGC ACCTGGATGG CCTGGCAACT TGACGAAAAT 
GCACAAAAAA TTCAAAGAGG CCAGATTCCT AGCAAATTTA TGATGGATCA GGAGTTTTAC 
CGTAGACACA GTATGACTGA ACTGAAGGAC GAAACGGGCT CCAGTAAAAC CCTCTCGGCT 
GCTGCACGCA GATCCTTCTT CCGCAGGAAA CACAAGCACA AACGCAGCAG CTCCAAAGAC 
GGGAAAGACG TAGTGGCTTT GGACGCCATC AGCACAGACT CAATTCCTTT CTTAGACGAC 
TGTGTGAGTC TGGCTTATCA GAGAGTGCAG AAGATTGACT GCACATCTCC CAGACCTGTG 
CTGATCCTCG GCCCGCTTGT GGATCCTGTT AAAGACATGC TGGTCAAAGA TTCTCCAGGG 
AAATTCAGCA GATGTGTTCT TGAGGTGATG AAGGCTTCTC AGCAGGCCAT TGAGCGTGGG 
GTCAAAGACT TTCTCTTCAT CGACTACAAA CGAAGGAGTG GCCATTTTGA TGTGACGACT 
GTTGCCTCAA TCAATGAAAT CACGGACAAG GACTGTCACT GTTTGCTTGA CATTGCACCT 
CATGCTATTG AAAGGCTTCA CAGCGTTCAC ATTTACCCAA TCGTCATTTT CATACGCTAC 
AAAAACGCAA AGCAGATAAA AGAGCAGAAG GACCCGATTT ATCTGCGGGA TAAAGTCTCT 
CAAAAACATT CCAAGGAGCA GTTTGAGATT GCACAGAAGA TTGAGCAAGA CTACAGCAAG 
TTCTTCACAG GTATCGTTCA GGGAGGCACC TTCCCTTACA TTTGCACTCA GATCGTGACT 
ATTGTGGATC AGGAACAGAG CAAAGTCTTG TGGACTCCAC TGGGCTCTCC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Genome wide association and genomic prediction for growth traits in juvenile farmed Atlantic salmon using a high density SNP array.
BMC genomics16969(2015 Nov)
Tsai HY,Hamilton A,Tinch AE,Guy DR,Gharbi K,Stear MJ,Matika O,Bishop SC,Houston RD

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Statistical evidence for a more than 800-million-year-old evolutionarily conserved genomic region in our genome.
Journal of molecular evolution59(5)587-97(2004 Nov)
Danchin EG,Pontarotti P

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dlg5a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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