SI:CH211-213A13.9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-213A13.9
Entrez Gene ID	569389
Full Name	si:ch211-213a13.9
General protein information	Preferred Names si:ch211-213a13.9 Names NACHT, LRR and PYD domains-containing protein 12 NACHT, LRR and PYD domains-containing protein 3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353233.2	XP_017208722.1	NACHT, LRR and PYD domains-containing protein 12 isoform X1
XM_021469437.1	XP_021325112.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
XM_017353233.1	XP_017208722.1	NACHT, LRR and PYD domains-containing protein 12

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565414	XP_693785.6
Danio rerio (zebrafish)	LOC558375	XP_686669.1
Danio rerio (zebrafish)	ftr29	XP_003198034.1
Danio rerio (zebrafish)	ftr82	NP_001068571.1
Danio rerio (zebrafish)	LOC560422	XP_688915.1
Danio rerio (zebrafish)	LOC100000832	XP_001340954.2
Danio rerio (zebrafish)	LOC100006636	XP_005172385.1
Danio rerio (zebrafish)	ftr73	XP_005157866.1
Danio rerio (zebrafish)	ftr11	XP_003197971.1
Danio rerio (zebrafish)	LOC101882560	XP_005163612.1
Danio rerio (zebrafish)	ftr67	NP_001034808.2
Danio rerio (zebrafish)	ftr34	XP_005163669.1
Danio rerio (zebrafish)	ftr37	XP_696445.3
Danio rerio (zebrafish)	ftr30	XP_003198033.2
Danio rerio (zebrafish)	btr22	XP_005173784.1
Danio rerio (zebrafish)	btr18	XP_005173787.1
Danio rerio (zebrafish)	btr32	XP_003201305.1
Danio rerio (zebrafish)	btr07	XP_001923237.4
Danio rerio (zebrafish)	LOC100001589	XP_001341559.3
Danio rerio (zebrafish)	ftr79	XP_697299.2
Danio rerio (zebrafish)	ftr83	XP_688583.3
Danio rerio (zebrafish)	LOC100333853	XP_002661845.3
Danio rerio (zebrafish)	si:dkeyp-123a12.2	XP_691204.4
Danio rerio (zebrafish)	LOC100007791	XP_001346158.5
Danio rerio (zebrafish)	si:ch211-250k18.8	XP_698776.5
Danio rerio (zebrafish)	zgc:174944	NP_001108057.1
Danio rerio (zebrafish)	LOC566957	XP_005174040.1
Danio rerio (zebrafish)	LOC100150225	XP_001921257.4
Danio rerio (zebrafish)	LOC100334101	XP_003197833.2
Danio rerio (zebrafish)	LOC100334843	XP_002665168.2
Danio rerio (zebrafish)	si:ch73-24k9.2	XP_002665507.3
Danio rerio (zebrafish)	si:ch211-196h16.5	XP_690098.5
Danio rerio (zebrafish)	si:ch211-194e1.7	XP_005167387.1
Danio rerio (zebrafish)	LOC793291	XP_001331884.5
Danio rerio (zebrafish)	LOC100004711	XP_001340175.3
Danio rerio (zebrafish)	LOC100534746	XP_005161332.1
Danio rerio (zebrafish)	LOC100005625	XP_001339269.4
Danio rerio (zebrafish)	LOC100003765	XP_001343238.2
Danio rerio (zebrafish)	LOC101883156	XP_005160435.1
Danio rerio (zebrafish)	si:ch211-237a4.2	XP_003198575.2
Danio rerio (zebrafish)	zgc:194906	NP_001124145.1
Danio rerio (zebrafish)	LOC100536980	XP_003201699.2
Danio rerio (zebrafish)	si:ch211-15j1.3	XP_002660683.2
Danio rerio (zebrafish)	si:ch211-233g6.6	XP_002660707.3
Danio rerio (zebrafish)	LOC100331097	XP_002661460.3
Danio rerio (zebrafish)	LOC101885873	XP_005161560.1
Danio rerio (zebrafish)	LOC101883187	XP_005174457.1
Danio rerio (zebrafish)	LOC101882512	XP_005171750.1
Danio rerio (zebrafish)	LOC100329306	XP_005168148.1
Danio rerio (zebrafish)	LOC100535915	XP_005161831.1
Danio rerio (zebrafish)	si:ch211-114l13.1	XP_002660672.3
Danio rerio (zebrafish)	LOC101885187	XP_005165034.1
Danio rerio (zebrafish)	si:rp71-7l19.2	XP_003198343.2
Danio rerio (zebrafish)	LOC100331680	XP_002667178.3
Danio rerio (zebrafish)	LOC100005516	XP_005171758.1
Danio rerio (zebrafish)	LOC101883084	XP_005174455.1
Danio rerio (zebrafish)	LOC101883426	XP_005174069.1
Danio rerio (zebrafish)	LOC100150280	XP_005174567.1
Danio rerio (zebrafish)	LOC101884637	XP_005168127.1
Danio rerio (zebrafish)	LOC100329543	XP_005167374.1
Danio rerio (zebrafish)	LOC101882306	XP_005171748.1
Danio rerio (zebrafish)	LOC566508	XP_694871.6
Danio rerio (zebrafish)	LOC101884187	XP_005157862.1
Danio rerio (zebrafish)	ftr55	XP_003200609.2
Danio rerio (zebrafish)	LOC100330813	XP_002660428.2
Danio rerio (zebrafish)	ftr49	XP_003198077.1
Danio rerio (zebrafish)	LOC100535633	XP_003198959.1
Danio rerio (zebrafish)	btr04	XP_003198956.2
Danio rerio (zebrafish)	btr09	NP_001124100.1
Danio rerio (zebrafish)	LOC558675	XP_687012.2
Danio rerio (zebrafish)	ftr76	XP_005157864.1
Danio rerio (zebrafish)	ftr16	XP_690796.5
Danio rerio (zebrafish)	LOC561829	XP_690320.2
Danio rerio (zebrafish)	ftr03	XP_002660930.2
Danio rerio (zebrafish)	ftr06	XP_001337856.1
Danio rerio (zebrafish)	LOC570185	XP_698724.1
Danio rerio (zebrafish)	LOC797531	XP_001338000.2
Danio rerio (zebrafish)	ftr02	XP_003197969.1
Danio rerio (zebrafish)	LOC571442	XP_700123.5
Danio rerio (zebrafish)	ftr19	XP_002660960.3
Danio rerio (zebrafish)	LOC101882488	XP_005163611.1
Danio rerio (zebrafish)	ftr39p	XP_002661035.2
Danio rerio (zebrafish)	zgc:136302	XP_005170221.1
Danio rerio (zebrafish)	ftr58	XP_686163.5
Danio rerio (zebrafish)	LOC100537283	XP_003198007.2
Danio rerio (zebrafish)	ftr64	XP_002661885.1
Danio rerio (zebrafish)	btr31	XP_005170663.1
Danio rerio (zebrafish)	LOC100006950	XP_001345543.5
Danio rerio (zebrafish)	ftr28l	XP_005163705.1
Danio rerio (zebrafish)	LOC100333325	XP_002667878.3
Danio rerio (zebrafish)	si:ch211-121a2.3	XP_698105.5
Danio rerio (zebrafish)	LOC799802	XP_001336792.2
Danio rerio (zebrafish)	LOC555438	XP_683030.6
Danio rerio (zebrafish)	LOC568716	XP_003201051.2
Danio rerio (zebrafish)	LOC568664	XP_003201050.2
Danio rerio (zebrafish)	si:ch73-286h23.3	XP_001346239.5
Danio rerio (zebrafish)	LOC100006645	XP_001337665.3
Danio rerio (zebrafish)	wu:fb15h11	XP_697949.6
Danio rerio (zebrafish)	si:ch211-191a16.2	XP_001338550.5
Danio rerio (zebrafish)	si:ch211-181d7.1	XP_001919795.3
Danio rerio (zebrafish)	LOC795182	XP_001332957.4
Danio rerio (zebrafish)	LOC100329836	XP_002664746.3
Danio rerio (zebrafish)	LOC100334547	XP_002664742.2
Danio rerio (zebrafish)	si:dkey-156m2.3	XP_002664795.3
Danio rerio (zebrafish)	si:ch211-213a13.6	XP_697815.6
Danio rerio (zebrafish)	si:dkey-13n15.11	XP_005165261.1
Danio rerio (zebrafish)	si:zfos-364h11.1	XP_002665163.2
Danio rerio (zebrafish)	LOC100002266	XP_005158672.1
Danio rerio (zebrafish)	si:ch211-278p9.3	XP_002665167.3
Danio rerio (zebrafish)	zgc:136791	XP_005160238.1
Danio rerio (zebrafish)	LOC796214	XP_005161889.1
Danio rerio (zebrafish)	LOC100536939	XP_003200134.2
Danio rerio (zebrafish)	zgc:174178	NP_001107787.1
Danio rerio (zebrafish)	LOC101882503	XP_005168090.1
Danio rerio (zebrafish)	si:ch211-114l13.4	XP_002660681.1
Danio rerio (zebrafish)	si:ch211-15j1.5	XP_002660682.2
Danio rerio (zebrafish)	LOC100334715	XP_005171097.1
Danio rerio (zebrafish)	si:ch211-15j1.1	XP_001920778.3
Danio rerio (zebrafish)	si:dkey-222h21.8	XP_002660686.2
Danio rerio (zebrafish)	si:dkey-222h21.5	XP_002660689.1
Danio rerio (zebrafish)	LOC101882744	XP_005168242.1
Danio rerio (zebrafish)	LOC100329650	XP_002661801.3
Danio rerio (zebrafish)	si:ch211-255g12.8	XP_694303.5
Danio rerio (zebrafish)	LOC100331576	XP_002661628.3
Danio rerio (zebrafish)	LOC101883760	XP_005164630.1
Danio rerio (zebrafish)	LOC101886147	XP_005167368.1
Danio rerio (zebrafish)	LOC101886579	XP_005161565.1
Danio rerio (zebrafish)	LOC100333957	XP_005171081.1
Danio rerio (zebrafish)	LOC101884123	XP_005171093.1
Danio rerio (zebrafish)	LOC101884874	XP_005171101.1
Danio rerio (zebrafish)	LOC100333264	XP_002660697.3
Danio rerio (zebrafish)	LOC100330744	XP_002660427.3
Danio rerio (zebrafish)	ftr50	XP_005164403.1
Danio rerio (zebrafish)	LOC793898	XP_001920428.2
Danio rerio (zebrafish)	LOC791496	XP_003198035.2
Danio rerio (zebrafish)	LOC565787	XP_694149.5
Danio rerio (zebrafish)	LOC570079	XP_698604.5
Danio rerio (zebrafish)	LOC570163	XP_003197979.1
Danio rerio (zebrafish)	btr16	XP_005157512.1
Danio rerio (zebrafish)	LOC570251	XP_698802.4
Danio rerio (zebrafish)	ftr15	XP_700104.3
Danio rerio (zebrafish)	ftr26	XP_005163617.1
Danio rerio (zebrafish)	ftr65	XP_003198031.2
Danio rerio (zebrafish)	zgc:173666	XP_003198037.1
Danio rerio (zebrafish)	ftr61	XP_003201315.1
Danio rerio (zebrafish)	btr20	XP_003200561.1
Danio rerio (zebrafish)	zgc:173581	XP_005173811.1
Danio rerio (zebrafish)	btr06	NP_001120951.1
Danio rerio (zebrafish)	LOC101886307	XP_005163673.1
Danio rerio (zebrafish)	LOC101884427	XP_005159614.1
Danio rerio (zebrafish)	LOC100002745	XP_005164415.1
Danio rerio (zebrafish)	LOC101885734	XP_005161559.1
Danio rerio (zebrafish)	si:dkey-151g22.1	NP_001137518.1
Danio rerio (zebrafish)	si:dkey-121n8.4	XP_001335920.4
Danio rerio (zebrafish)	LOC100330073	XP_002661806.3
Danio rerio (zebrafish)	si:dkey-7i4.1	XP_005169721.1
Danio rerio (zebrafish)	LOC100001465	XP_005166707.1
Danio rerio (zebrafish)	LOC100005483	XP_001339130.3
Danio rerio (zebrafish)	LOC562679	XP_691146.5
Danio rerio (zebrafish)	LOC100333840	XP_005169858.1
Danio rerio (zebrafish)	LOC100536924	XP_003200567.2
Danio rerio (zebrafish)	LOC559196	XP_687605.5
Danio rerio (zebrafish)	LOC795109	XP_001335224.5
Danio rerio (zebrafish)	si:ch211-187g4.1	XP_698878.6
Danio rerio (zebrafish)	LOC100538217	XP_005169783.1
Danio rerio (zebrafish)	LOC571651	XP_700351.5
Danio rerio (zebrafish)	si:ch211-271g18.1	XP_002661463.3
Danio rerio (zebrafish)	LOC565381	XP_693750.6
Danio rerio (zebrafish)	si:ch211-238e22.7	XP_005168160.1
Danio rerio (zebrafish)	LOC100333747	XP_002661530.3
Danio rerio (zebrafish)	LOC100331072	XP_002661905.3
Danio rerio (zebrafish)	si:ch73-236c18.7	XP_002660699.3
Danio rerio (zebrafish)	si:dkey-222h21.6	XP_002660688.2
Danio rerio (zebrafish)	si:ch211-233g6.3	XP_002660709.3
Danio rerio (zebrafish)	LOC100329354	XP_002661501.3
Danio rerio (zebrafish)	LOC100006280	XP_001920132.4
Danio rerio (zebrafish)	LOC101886178	XP_005164994.1
Danio rerio (zebrafish)	LOC101883660	XP_005168155.1
Danio rerio (zebrafish)	LOC101883625	XP_005170693.1
Danio rerio (zebrafish)	LOC100150791	XP_005165031.1
Danio rerio (zebrafish)	LOC101883234	XP_005160282.1
Danio rerio (zebrafish)	LOC101885938	XP_005173831.1
Danio rerio (zebrafish)	LOC101885252	XP_005168541.1
Danio rerio (zebrafish)	trpm2	XP_003198602.2
Danio rerio (zebrafish)	btr12	NP_001070087.1
Danio rerio (zebrafish)	btr02	NP_001070626.2
Danio rerio (zebrafish)	ftr72	NP_001007388.2
Danio rerio (zebrafish)	LOC101883527	XP_005170222.1
Danio rerio (zebrafish)	trim25l	XP_005172141.1
Danio rerio (zebrafish)	LOC100149991	XP_003200554.1
Danio rerio (zebrafish)	LOC101883221	XP_005171452.1
Danio rerio (zebrafish)	ftr56	XP_003200605.1
Danio rerio (zebrafish)	LOC100334710	XP_002665516.2
Danio rerio (zebrafish)	btr25	XP_005173808.1
Danio rerio (zebrafish)	ftr24	XP_003197988.1
Danio rerio (zebrafish)	LOC100536343	XP_003197990.1
Danio rerio (zebrafish)	LOC100537128	XP_003200077.2
Danio rerio (zebrafish)	LOC100536410	XP_003200565.2
Danio rerio (zebrafish)	btr21	XP_003200564.2
Danio rerio (zebrafish)	ftr84	XP_005165427.1
Danio rerio (zebrafish)	si:ch211-150h8.3	XP_003198032.1
Danio rerio (zebrafish)	ftr43	XP_005164401.1
Danio rerio (zebrafish)	LOC571488	XP_700178.4
Danio rerio (zebrafish)	ftr41	XP_695494.5
Danio rerio (zebrafish)	ftr59	XP_686796.2
Danio rerio (zebrafish)	ftr51	XP_691169.5
Danio rerio (zebrafish)	ftr14l	XP_003199766.1
Danio rerio (zebrafish)	si:ch211-214b16.3	XP_005164324.1
Danio rerio (zebrafish)	LOC100331532	XP_003201602.2
Danio rerio (zebrafish)	LOC100329480	XP_002663890.3
Danio rerio (zebrafish)	si:dkey-222h21.2	XP_001334961.4
Danio rerio (zebrafish)	LOC100006564	NP_001119957.1
Danio rerio (zebrafish)	LOC100333746	XP_002661485.3
Danio rerio (zebrafish)	LOC100329448	XP_002667651.3
Danio rerio (zebrafish)	si:ch211-114l13.10	XP_696367.5
Danio rerio (zebrafish)	LOC100331159	XP_005169797.1
Danio rerio (zebrafish)	si:ch211-213a13.9	XP_003201049.2
Danio rerio (zebrafish)	si:dkey-253d23.10	XP_683789.6
Danio rerio (zebrafish)	si:ch211-278p9.4	XP_001336197.5
Danio rerio (zebrafish)	LOC566782	XP_695160.6
Danio rerio (zebrafish)	LOC558818	NP_001038341.1
Danio rerio (zebrafish)	si:ch211-149a19.3	XP_005170041.1
Danio rerio (zebrafish)	si:dkey-222h21.9	XP_002660685.1
Danio rerio (zebrafish)	LOC796962	XP_001923433.2
Danio rerio (zebrafish)	LOC100536895	XP_003200133.2
Danio rerio (zebrafish)	LOC100537076	XP_003200135.2
Danio rerio (zebrafish)	LOC100329927	XP_003200136.2
Danio rerio (zebrafish)	si:ch211-233m11.1	XP_687146.4
Danio rerio (zebrafish)	LOC100535360	XP_003200157.2
Danio rerio (zebrafish)	LOC101884992	XP_005174036.1
Danio rerio (zebrafish)	LOC794214	XP_001334125.1
Danio rerio (zebrafish)	LOC100537399	XP_003198502.2
Danio rerio (zebrafish)	LOC100007745	XP_001346125.5
Danio rerio (zebrafish)	si:dkey-126g1.7	XP_005162400.1
Danio rerio (zebrafish)	LOC101882643	XP_005171752.1
Danio rerio (zebrafish)	LOC100001765	XP_001341704.4
Danio rerio (zebrafish)	si:dkey-82i20.1	XP_002666374.3
Danio rerio (zebrafish)	LOC100333748	XP_002661966.3
Danio rerio (zebrafish)	LOC565296	XP_003198495.2
Danio rerio (zebrafish)	LOC100535428	XP_005174493.1
Danio rerio (zebrafish)	LOC100334677	XP_002661542.3
Danio rerio (zebrafish)	LOC100329558	XP_002661549.3
Danio rerio (zebrafish)	si:dkeyp-4c4.1	NP_001094426.1
Danio rerio (zebrafish)	LOC101885760	XP_005164976.1
Danio rerio (zebrafish)	LOC101884884	XP_005157350.1
Danio rerio (zebrafish)	LOC101885212	XP_005158663.1
Danio rerio (zebrafish)	LOC101885461	XP_005168195.1
Danio rerio (zebrafish)	LOC560232	NP_001116726.1
Danio rerio (zebrafish)	LOC101885169	XP_005174039.1
Danio rerio (zebrafish)	LOC101883611	XP_005174070.1

Gene Ontology

The following SI:CH211-213A13.9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-213A13.9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151260	XM_021469437.1 Latest version!	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa57106	XM_017353233.2 Latest version!	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa57106	XM_017353233.1	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-213A13.9 ( XM_021469437.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325112.1 Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa151260
Clone ID Related Accession (Same CDS sequence)	XM_021469437.1
Accession Version	XM_021469437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAAGTTATT GAGTCCAGCT GCTGATGAAG CCTGTTCGTT TGTGGCTGTA 
CATGTGGGTG AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTAATTA TGAACTAGGA 
GACCCACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTCATGCTCA GTAATTGTGG TCTAACAGAG GAAAGCTGTT CAGCTCTTGC TTCAGTTCTC 
AGATCAGACT CCTGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGAATTCA 
GGAGTGAAGC GGCTCTCTGA TGGACTGAAG GACAACTGTA AATTGGAGAA ACTCAGACTA 
TCAGACTGCA GTATCAGTGA AGGAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CTTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTCTTAGT 
TCTGCTGTAG AGGAAGTCTG TAAACATTTG TCTGATGTTC TCACTGGGGA CCCGTTAATC 
CTGAAAGAAC TGAATCTGGG TGGATGTAAA ATAGAAGACA AAGTGAGTTA TCTTGCTACT 
CTACTGCAGG ATAAACACTG TCAGATCAGC ACACTGGTGC TGAACAATAC CAGTATTACA 
GAAGAGGGTT GTGCTAATCT GACTTCAGCA ATTACGTTAA ACCCATCACA CCTGAGGGAA 
CTGAACCTAA GTGATAATAA ACTAGGAGAC TCTGCAGTGA AAAACCTCAG TGATTTACTG 
ATAAAGACAC AATTCAAGCT GGAGAAACTA CATTTGAGTA ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGTGA ATGAAATGGG AGACTCTGCA GTGAAAAACT TAAGGGATTT TCTGATGAAG 
CCACAATGCA ATCTGGAGAA ACTACTTCTG CGTCAATGCA GTATTACAGA GAAGCAGTGT 
GAGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GGACCTCAGT 
AGAAATGAAA TAAACAACAC AGGAGTAAAT CTTTTATCTG AAGTACTGAA GGATTCACAC 
TGTAAACTGG AGAGATTGAG TCTAATTGAA TGTGGCATCA CAGATGTTTC CTCTTTTGCT 
CACAATTTGA TGAACACAGA AACCCTGAAA GAGCTTGATC TTAGAAACAA TTCTATATTA 
ATTTCAAGGC GGAAACTGTT ATATGTGCTA TCAGACTCTA ACTGTAAACT GAGCCTAGAC 
AAGGGTAACT TGCATCAACG TTTTGCTGAA TTATTTTTTG ATAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325112.1
CDS	251..5281
Translation	MSEMEGASPKMSLPEKHRMRSGSHAPSSVSMKSDWSMEKPPDLTSEGKPASTTSFLSLKSDWSMEKPPDLTCKRKLMSTKRLLSETQDFQAHKNHKNFTDNLQEIFKNLESQIISFLTNEFEMFKKILLRVNTNNFVKDFNENRCSIREAALDLTLYFLREMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTKHEVRQIEVTSRHHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPVSLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEVRGFNDEQKVEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNFLQEKRNNDVKHNQADSKTLQESNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFQQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKKETGIVLGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSRNRNESMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNRRLLRGLFAQHDHNNKWNIWSIWKIFQYFFRTADQQQDHNDLSKEEIVQYLKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTRLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTSKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDNCKLEKLGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDALLDTKCKAKLRFLSSAADEFCQFVTGIMGKNPLLLKELNLSDRELGDTRVNQLSDLLQDKHCQINTLMLKNNRITAEGCADLMSAFNSNPSHLTELNLSVNELGDLGVEKICPLLENTLCRLEKLNLSDCSITGQSYETLASVLRSNLYLKELDLRGNDPGKSGVKKFIDFLQEGSCKLQIIRFLKSPDAEEACEYLTKDLVINPLLLTELDLSKVKLGHLDGEKFSAFLMDSHNKVEKIKLNNCKLTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVRKICEGLKNPVCELKILKLLSPAADEACSFVAVHVGENPLLLKELNLSNYELGDPRVNQLSALLQDKHCKLNTLMLSNCGLTEESCSALASVLRSDSCSLKDLDLSNNNLQNSGVKRLSDGLKDNCKLEKLRLSDCSISEGGYKALASALRSNLSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSSAVEEVCKHLSDVLTGDPLILKELNLGGCKIEDKVSYLATLLQDKHCQISTLVLNNTSITEEGCANLTSAITLNPSHLRELNLSDNKLGDSAVKNLSDLLIKTQFKLEKLHLSNCSITEEQCVILTSALKSNPSHLRELNLSVNEMGDSAVKNLRDFLMKPQCNLEKLLLRQCSITEKQCEILTSALKSNPSHLRELDLSRNEINNTGVNLLSEVLKDSHCKLERLSLIECGITDVSSFAHNLMNTETLKELDLRNNSILISRRKLLYVLSDSNCKLSLDKGNLHQRFAELFFDNQ

Target ORF information:

RefSeq Version	XM_021469437.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469437.1

ORF Insert Sequence:

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAAGTTATT GAGTCCAGCT GCTGATGAAG CCTGTTCGTT TGTGGCTGTA 
CATGTGGGTG AAAACCCGTT ACTCCTGAAA GAACTGAATC TGAGTAATTA TGAACTAGGA 
GACCCACGAG TGAATCAACT CTCTGCTCTA CTGCAGGATA AACACTGTAA ACTCAACACA 
CTCATGCTCA GTAATTGTGG TCTAACAGAG GAAAGCTGTT CAGCTCTTGC TTCAGTTCTC 
AGATCAGACT CCTGCAGTCT GAAGGATCTT GACCTGAGCA ATAATAATCT GCAGAATTCA 
GGAGTGAAGC GGCTCTCTGA TGGACTGAAG GACAACTGTA AATTGGAGAA ACTCAGACTA 
TCAGACTGCA GTATCAGTGA AGGAGGTTAT AAAGCTCTGG CTTCAGCTCT GAGATCAAAC 
CTTTCACACC TGATAGAGCT GGATCTCAGA GGAAATGATC CTGGACAATC AGGAGTGAAG 
GAGCTCACTG ACTTACTGCA GGATCCACAC TGTACACTGA AGACACTGAG GTTTCTTAGT 
TCTGCTGTAG AGGAAGTCTG TAAACATTTG TCTGATGTTC TCACTGGGGA CCCGTTAATC 
CTGAAAGAAC TGAATCTGGG TGGATGTAAA ATAGAAGACA AAGTGAGTTA TCTTGCTACT 
CTACTGCAGG ATAAACACTG TCAGATCAGC ACACTGGTGC TGAACAATAC CAGTATTACA 
GAAGAGGGTT GTGCTAATCT GACTTCAGCA ATTACGTTAA ACCCATCACA CCTGAGGGAA 
CTGAACCTAA GTGATAATAA ACTAGGAGAC TCTGCAGTGA AAAACCTCAG TGATTTACTG 
ATAAAGACAC AATTCAAGCT GGAGAAACTA CATTTGAGTA ATTGCAGTAT TACAGAGGAA 
CAGTGTGTGA TCCTGACTTC AGCTCTGAAA TCAAACCCAT CACACCTGAG AGAACTGAAC 
CTCAGTGTGA ATGAAATGGG AGACTCTGCA GTGAAAAACT TAAGGGATTT TCTGATGAAG 
CCACAATGCA ATCTGGAGAA ACTACTTCTG CGTCAATGCA GTATTACAGA GAAGCAGTGT 
GAGATCCTGA CTTCAGCTCT GAAATCAAAC CCATCACACC TGAGAGAACT GGACCTCAGT 
AGAAATGAAA TAAACAACAC AGGAGTAAAT CTTTTATCTG AAGTACTGAA GGATTCACAC 
TGTAAACTGG AGAGATTGAG TCTAATTGAA TGTGGCATCA CAGATGTTTC CTCTTTTGCT 
CACAATTTGA TGAACACAGA AACCCTGAAA GAGCTTGATC TTAGAAACAA TTCTATATTA 
ATTTCAAGGC GGAAACTGTT ATATGTGCTA TCAGACTCTA ACTGTAAACT GAGCCTAGAC 
AAGGGTAACT TGCATCAACG TTTTGCTGAA TTATTTTTTG ATAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-213A13.9 ( XM_017353233.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208722.1 Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X1, mRNA.

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CloneID	ODa57106
Clone ID Related Accession (Same CDS sequence)	XM_017353233.2 , XM_017353233.1
Accession Version	XM_017353233.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353233.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAATCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCTTTAAT GTCCTACAGG ATCCTCAGTT TAAAACAAAA 
ATTAGGTTAT TGAGTCCAGC TGCTGATGAA GCCTGTTCGT TTGTGGCTGT ACATGTGGGT 
GAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTAATT ATGAACTAGG AGACCCACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTC 
AGTAATTGTG GTCTAACAGA GGAAAGCTGT TCAGCTCTTG CTTCAGTTCT CAGATCAGAC 
TCCTGCAGTC TGAAGGATCT TGACCTGAGC AATAATAATC TGCAGAATTC AGGAGTGAAG 
CGGCTCTCTG ATGGACTGAA GGACAACTGT AAATTGGAGA AACTCAGACT ATCAGACTGC 
AGTATCAGTG AAGGAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCTTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTCTTAG TTCTGCTGTA 
GAGGAAGTCT GTAAACATTT GTCTGATGTT CTCACTGGGG ACCCGTTAAT CCTGAAAGAA 
CTGAATCTGG GTGGATGTAA AATAGAAGAC AAAGTGAGTT ATCTTGCTAC TCTACTGCAG 
GATAAACACT GTCAGATCAG CACACTGGTG CTGAACAATA CCAGTATTAC AGAAGAGGGT 
TGTGCTAATC TGACTTCAGC AATTACGTTA AACCCATCAC ACCTGAGGGA ACTGAACCTA 
AGTGATAATA AACTAGGAGA CTCTGCAGTG AAAAACCTCA GTGATTTACT GATAAAGACA 
CAATTCAAGC TGGAGAAACT ACATTTGAGT AATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGTG 
AATGAAATGG GAGACTCTGC AGTGAAAAAC TTAAGGGATT TTCTGATGAA GCCACAATGC 
AATCTGGAGA AACTACTTCT GCGTCAATGC AGTATTACAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TAGAAATGAA 
ATAAACAACA CAGGAGTAAA TCTTTTATCT GAAGTACTGA AGGATTCACA CTGTAAACTG 
GAGAGATTGA GTCTAATTGA ATGTGGCATC ACAGATGTTT CCTCTTTTGC TCACAATTTG 
ATGAACACAG AAACCCTGAA AGAGCTTGAT CTTAGAAACA ATTCTATATT AATTTCAAGG 
CGGAAACTGT TATATGTGCT ATCAGACTCT AACTGTAAAC TGAGCCTAGA CAAGGGTAAC 
TTGCATCAAC GTTTTGCTGA ATTATTTTTT GATAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208722.1
CDS	251..5452
Translation	MSEMEGASPKMSLPEKHRMRSGSHAPSSVSMKSDWSMEKPPDLTSEGKPASTTSFLSLKSDWSMEKPPDLTCKRKLMSTKRLLSETQDFQAHKNHKNFTDNLQEIFKNLESQIISFLTNEFEMFKKILLRVNTNNFVKDFNENRCSIREAALDLTLYFLREMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTKHEVRQIEVTSRHHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPVSLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEVRGFNDEQKVEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNFLQEKRNNDVKHNQADSKTLQESNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFQQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKKETGIVLGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSRNRNESMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNRRLLRGLFAQHDHNNKWNIWSIWKIFQYFFRTADQQQDHNDLSKEEIVQYLKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTRLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTSKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDNCKLEKLGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDALLDTKCKAKLRFLSSAADEFCQFVTGIMGKNPLLLKELNLSDRELGDTRVNQLSDLLQDKHCQINTLMLKNNRITAEGCADLMSAFNSNPSHLTELNLSVNELGDLGVEKICPLLENTLCRLEKLNLSDCSITGQSYETLASVLRSNLYLKELDLRGNDPGKSGVKKFIDFLQEGSCKLQIIRFLKSPDAEEACEYLTKDLVINPLLLTELDLSKVKLGHLDGEKFSAFLMDSHNKVEKIKLNNCKLTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELFNVLQDPQFKTKIRLLSPAADEACSFVAVHVGENPLLLKELNLSNYELGDPRVNQLSALLQDKHCKLNTLMLSNCGLTEESCSALASVLRSDSCSLKDLDLSNNNLQNSGVKRLSDGLKDNCKLEKLRLSDCSISEGGYKALASALRSNLSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSSAVEEVCKHLSDVLTGDPLILKELNLGGCKIEDKVSYLATLLQDKHCQISTLVLNNTSITEEGCANLTSAITLNPSHLRELNLSDNKLGDSAVKNLSDLLIKTQFKLEKLHLSNCSITEEQCVILTSALKSNPSHLRELNLSVNEMGDSAVKNLRDFLMKPQCNLEKLLLRQCSITEKQCEILTSALKSNPSHLRELDLSRNEINNTGVNLLSEVLKDSHCKLERLSLIECGITDVSSFAHNLMNTETLKELDLRNNSILISRRKLLYVLSDSNCKLSLDKGNLHQRFAELFFDNQ

Target ORF information:

RefSeq Version	XM_017353233.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353233.2

ORF Insert Sequence:

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAATCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCTTTAAT GTCCTACAGG ATCCTCAGTT TAAAACAAAA 
ATTAGGTTAT TGAGTCCAGC TGCTGATGAA GCCTGTTCGT TTGTGGCTGT ACATGTGGGT 
GAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTAATT ATGAACTAGG AGACCCACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTC 
AGTAATTGTG GTCTAACAGA GGAAAGCTGT TCAGCTCTTG CTTCAGTTCT CAGATCAGAC 
TCCTGCAGTC TGAAGGATCT TGACCTGAGC AATAATAATC TGCAGAATTC AGGAGTGAAG 
CGGCTCTCTG ATGGACTGAA GGACAACTGT AAATTGGAGA AACTCAGACT ATCAGACTGC 
AGTATCAGTG AAGGAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCTTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTCTTAG TTCTGCTGTA 
GAGGAAGTCT GTAAACATTT GTCTGATGTT CTCACTGGGG ACCCGTTAAT CCTGAAAGAA 
CTGAATCTGG GTGGATGTAA AATAGAAGAC AAAGTGAGTT ATCTTGCTAC TCTACTGCAG 
GATAAACACT GTCAGATCAG CACACTGGTG CTGAACAATA CCAGTATTAC AGAAGAGGGT 
TGTGCTAATC TGACTTCAGC AATTACGTTA AACCCATCAC ACCTGAGGGA ACTGAACCTA 
AGTGATAATA AACTAGGAGA CTCTGCAGTG AAAAACCTCA GTGATTTACT GATAAAGACA 
CAATTCAAGC TGGAGAAACT ACATTTGAGT AATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGTG 
AATGAAATGG GAGACTCTGC AGTGAAAAAC TTAAGGGATT TTCTGATGAA GCCACAATGC 
AATCTGGAGA AACTACTTCT GCGTCAATGC AGTATTACAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TAGAAATGAA 
ATAAACAACA CAGGAGTAAA TCTTTTATCT GAAGTACTGA AGGATTCACA CTGTAAACTG 
GAGAGATTGA GTCTAATTGA ATGTGGCATC ACAGATGTTT CCTCTTTTGC TCACAATTTG 
ATGAACACAG AAACCCTGAA AGAGCTTGAT CTTAGAAACA ATTCTATATT AATTTCAAGG 
CGGAAACTGT TATATGTGCT ATCAGACTCT AACTGTAAAC TGAGCCTAGA CAAGGGTAAC 
TTGCATCAAC GTTTTGCTGA ATTATTTTTT GATAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-213A13.9 ( XM_017353233.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208722.1 Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), mRNA.

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CloneID	ODa57106
Clone ID Related Accession (Same CDS sequence)	XM_017353233.2 , XM_017353233.1
Accession Version	XM_017353233.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAATCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCTTTAAT GTCCTACAGG ATCCTCAGTT TAAAACAAAA 
ATTAGGTTAT TGAGTCCAGC TGCTGATGAA GCCTGTTCGT TTGTGGCTGT ACATGTGGGT 
GAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTAATT ATGAACTAGG AGACCCACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTC 
AGTAATTGTG GTCTAACAGA GGAAAGCTGT TCAGCTCTTG CTTCAGTTCT CAGATCAGAC 
TCCTGCAGTC TGAAGGATCT TGACCTGAGC AATAATAATC TGCAGAATTC AGGAGTGAAG 
CGGCTCTCTG ATGGACTGAA GGACAACTGT AAATTGGAGA AACTCAGACT ATCAGACTGC 
AGTATCAGTG AAGGAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCTTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTCTTAG TTCTGCTGTA 
GAGGAAGTCT GTAAACATTT GTCTGATGTT CTCACTGGGG ACCCGTTAAT CCTGAAAGAA 
CTGAATCTGG GTGGATGTAA AATAGAAGAC AAAGTGAGTT ATCTTGCTAC TCTACTGCAG 
GATAAACACT GTCAGATCAG CACACTGGTG CTGAACAATA CCAGTATTAC AGAAGAGGGT 
TGTGCTAATC TGACTTCAGC AATTACGTTA AACCCATCAC ACCTGAGGGA ACTGAACCTA 
AGTGATAATA AACTAGGAGA CTCTGCAGTG AAAAACCTCA GTGATTTACT GATAAAGACA 
CAATTCAAGC TGGAGAAACT ACATTTGAGT AATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGTG 
AATGAAATGG GAGACTCTGC AGTGAAAAAC TTAAGGGATT TTCTGATGAA GCCACAATGC 
AATCTGGAGA AACTACTTCT GCGTCAATGC AGTATTACAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TAGAAATGAA 
ATAAACAACA CAGGAGTAAA TCTTTTATCT GAAGTACTGA AGGATTCACA CTGTAAACTG 
GAGAGATTGA GTCTAATTGA ATGTGGCATC ACAGATGTTT CCTCTTTTGC TCACAATTTG 
ATGAACACAG AAACCCTGAA AGAGCTTGAT CTTAGAAACA ATTCTATATT AATTTCAAGG 
CGGAAACTGT TATATGTGCT ATCAGACTCT AACTGTAAAC TGAGCCTAGA CAAGGGTAAC 
TTGCATCAAC GTTTTGCTGA ATTATTTTTT GATAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208722.1
CDS	250..5451
Translation	MSEMEGASPKMSLPEKHRMRSGSHAPSSVSMKSDWSMEKPPDLTSEGKPASTTSFLSLKSDWSMEKPPDLTCKRKLMSTKRLLSETQDFQAHKNHKNFTDNLQEIFKNLESQIISFLTNEFEMFKKILLRVNTNNFVKDFNENRCSIREAALDLTLYFLREMKQDEAADTLEDELFFIHQLKCGLQKKYQCVFEGIAQQGDSTLLKNIYTDLYITQGGSEQVNTKHEVRQIEVTSRHHEAQEIQVECRHLFEASEQDKQIRTVLTKGVAGIGKSVSVQKFVLDWAEGKENQEISFIFPLPFREMNLKEKETLSLMELITQFYPETKGLNLTRRNRFKVLFILDGLDECRLPLNFAGNETCCDVSSPVSLDVLLTNLIKGNLLPSALIWITSRPAAASKIPPDCIDRVTEVRGFNDEQKVEYFRKRLTDENQAKEIIDHVKQSKSLFIMCHIPVFCWISATVLQNFLQEKRNNDVKHNQADSKTLQESNTEDTPKTLTQMYTHFLRFQIQQSRRKYDGENTPDVSWDKDAILSLGKLAFQQLLNSNLIFYDTDLEACGIDVYKASVYSGMCTQIFKKETGIVLGTMYCFVHLSVQEFIAALYAHLILDINKKSVFDQDSRNRNESMIDLLKTAVDKALESDNGHLDLFLRFLLGLSLQSNRRLLRGLFAQHDHNNKWNIWSIWKIFQYFFRTADQQQDHNDLSKEEIVQYLKQKFEANLSPERSINLFYCLNELNDQTLVKEIQTRLSKGSLSSADLSPAQWSALAFVLLTSEEEMEEFELQKFKKSDECLIRLSAVIKTSKRALLSKCNLTEESCSALASVLRSDSSSLKDLDLSNNNLQDSGVKLLSDGLKDNCKLEKLGLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGPSGVKELTDALLDTKCKAKLRFLSSAADEFCQFVTGIMGKNPLLLKELNLSDRELGDTRVNQLSDLLQDKHCQINTLMLKNNRITAEGCADLMSAFNSNPSHLTELNLSVNELGDLGVEKICPLLENTLCRLEKLNLSDCSITGQSYETLASVLRSNLYLKELDLRGNDPGKSGVKKFIDFLQEGSCKLQIIRFLKSPDAEEACEYLTKDLVINPLLLTELDLSKVKLGHLDGEKFSAFLMDSHNKVEKIKLNNCKLTEKSCSVLAAVLSSKTILKEMNLNNSRLLDSGVRKICEGLKNPVCELKILNLSDCSISEEGYKALASALRSNPSHLIELDLRGNDPGQSGVKELFNVLQDPQFKTKIRLLSPAADEACSFVAVHVGENPLLLKELNLSNYELGDPRVNQLSALLQDKHCKLNTLMLSNCGLTEESCSALASVLRSDSCSLKDLDLSNNNLQNSGVKRLSDGLKDNCKLEKLRLSDCSISEGGYKALASALRSNLSHLIELDLRGNDPGQSGVKELTDLLQDPHCTLKTLRFLSSAVEEVCKHLSDVLTGDPLILKELNLGGCKIEDKVSYLATLLQDKHCQISTLVLNNTSITEEGCANLTSAITLNPSHLRELNLSDNKLGDSAVKNLSDLLIKTQFKLEKLHLSNCSITEEQCVILTSALKSNPSHLRELNLSVNEMGDSAVKNLRDFLMKPQCNLEKLLLRQCSITEKQCEILTSALKSNPSHLRELDLSRNEINNTGVNLLSEVLKDSHCKLERLSLIECGITDVSSFAHNLMNTETLKELDLRNNSILISRRKLLYVLSDSNCKLSLDKGNLHQRFAELFFDNQ

Target ORF information:

RefSeq Version	XM_017353233.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-213a13.9 (si:ch211-213a13.9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353233.1

ORF Insert Sequence:

ATGTCAGAGA TGGAAGGGGC CTCTCCTAAG ATGAGTCTCC CAGAGAAACA CCGTATGAGA 
TCAGGTTCAC ATGCACCCAG CTCTGTGTCT ATGAAGAGTG ATTGGTCAAT GGAAAAACCA 
CCAGACCTGA CAAGTGAGGG GAAACCTGCA TCTACCACAA GCTTTTTGTC TCTGAAGAGT 
GACTGGTCGA TGGAAAAACC ACCAGACCTG ACCTGTAAAA GAAAACTCAT GTCTACCAAA 
AGGCTTTTAT CTGAGACACA AGATTTCCAG GCTCACAAAA ACCACAAGAA TTTTACAGAC 
AATCTTCAAG AGATTTTCAA GAATCTGGAA AGCCAAATAA TCTCATTCCT TACCAACGAG 
TTTGAAATGT TTAAAAAAAT ATTACTAAGA GTAAACACAA ATAATTTTGT AAAGGATTTT 
AATGAGAACA GATGCAGCAT CAGAGAAGCA GCTCTTGATC TCACACTCTA CTTCCTGAGA 
GAAATGAAGC AAGATGAAGC TGCAGATACT CTAGAAGATG AGCTGTTCTT CATTCATCAG 
CTGAAATGTG GCCTACAGAA GAAGTATCAG TGTGTGTTTG AAGGAATTGC ACAGCAAGGA 
GACTCCACAC TCCTGAAGAA CATCTACACA GATCTCTATA TCACTCAGGG TGGCAGTGAA 
CAGGTCAATA CTAAACATGA GGTCAGACAG ATTGAAGTTA CTTCTAGACA CCATGAAGCT 
CAAGAGATAC AGGTTGAATG CAGACATTTG TTTGAAGCGT CTGAACAAGA CAAGCAGATC 
AGAACTGTAC TGACAAAAGG AGTCGCTGGC ATCGGGAAAT CAGTCTCTGT GCAAAAGTTT 
GTTCTGGATT GGGCTGAAGG AAAGGAAAAT CAAGAGATCA GCTTCATATT TCCTCTTCCA 
TTCAGAGAGA TGAACTTAAA GGAGAAAGAA ACACTCAGTT TGATGGAGCT TATAACTCAG 
TTTTACCCAG AGACAAAAGG ACTGAACCTT ACAAGAAGGA ATCGATTCAA AGTCCTGTTC 
ATCCTTGATG GATTGGATGA ATGTCGTCTT CCTCTGAACT TTGCTGGTAA TGAGACGTGT 
TGTGATGTTT CGTCACCAGT CTCTCTGGAT GTTCTCCTGA CGAATCTCAT CAAGGGAAAT 
CTGCTTCCTT CTGCTCTCAT CTGGATCACC AGCAGACCAG CAGCTGCCAG TAAGATTCCT 
CCTGACTGTA TCGACAGAGT GACAGAAGTT AGAGGATTCA ATGATGAACA AAAGGTCGAG 
TACTTCAGAA AAAGACTCAC AGATGAGAAT CAGGCCAAAG AAATCATTGA TCATGTTAAA 
CAATCAAAGA GTCTCTTCAT CATGTGCCAC ATCCCAGTCT TCTGCTGGAT TTCAGCCACT 
GTTCTCCAGA ACTTTTTACA GGAGAAGAGA AATAATGATG TGAAACACAA TCAGGCTGAT 
TCCAAAACAC TGCAGGAATC AAACACTGAA GACACTCCCA AGACTCTGAC ACAGATGTAC 
ACACACTTTC TGCGCTTTCA GATCCAGCAG AGCAGACGAA AGTATGATGG AGAAAACACA 
CCAGATGTGT CCTGGGATAA AGACGCCATC CTTTCACTGG GGAAACTGGC ATTTCAGCAG 
CTGCTAAACA GCAACCTGAT CTTCTATGAC ACAGACCTGG AAGCCTGTGG TATTGACGTC 
TATAAGGCCT CAGTTTACTC AGGCATGTGT ACCCAGATCT TTAAGAAGGA AACAGGGATT 
GTTCTTGGTA CCATGTACTG CTTTGTTCAC TTGAGCGTTC AAGAGTTTAT TGCAGCCCTT 
TATGCACATC TGATTCTAGA CATCAACAAG AAAAGTGTGT TTGATCAAGA CTCTAGAAAC 
AGAAATGAGT CCATGATTGA TTTGCTGAAG ACTGCAGTGG ACAAGGCACT TGAGAGTGAC 
AATGGACACC TGGACCTTTT CCTTCGCTTT CTTCTTGGTC TGTCTCTCCA GTCAAATCGG 
CGACTCTTAC GAGGTCTGTT TGCACAGCAC GACCACAATA ACAAATGGAA CATATGGAGC 
ATATGGAAAA TATTTCAGTA CTTCTTTAGA ACAGCAGATC AGCAGCAAGA CCACAATGAC 
CTTAGCAAAG AGGAAATAGT TCAGTACCTC AAGCAGAAGT TTGAAGCGAA TCTTTCTCCA 
GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGACCAAAC TCTGGTGAAA 
GAAATTCAGA CTCGCCTTAG TAAAGGAAGT CTCTCATCTG CTGATCTTTC ACCTGCCCAG 
TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGAGGAGG AGATGGAGGA GTTTGAGCTC 
CAGAAATTCA AGAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTCC 
AAAAGAGCTC TGCTCAGTAA GTGTAATCTA ACAGAGGAAA GCTGTTCAGC TCTTGCTTCA 
GTTCTCAGAT CAGACTCCAG CAGTCTGAAG GATCTTGACC TGAGCAATAA TAATCTGCAG 
GATTCAGGAG TGAAGCTGCT CTCTGATGGA CTGAAGGACA ACTGTAAATT GGAGAAACTC 
GGACTATCAG ACTGCAGTAT CAGTGAAGAA GGTTATAAAG CTCTGGCTTC AGCTCTGAGA 
TCAAACCCTT CACACCTGAT AGAGCTGGAT CTCAGAGGAA ATGATCCTGG ACCATCAGGA 
GTGAAGGAGC TCACTGATGC ATTACTGGAT ACAAAGTGTA AAGCAAAACT GAGGTTTTTG 
AGTTCTGCTG CTGATGAATT CTGTCAGTTT GTGACTGGAA TTATGGGTAA AAACCCGTTA 
CTCCTGAAAG AACTGAATCT GAGTGATCGT GAACTAGGAG ACACACGAGT GAATCAACTC 
TCTGATCTAC TGCAGGACAA ACACTGTCAG ATCAACACAC TCATGCTGAA GAATAACAGG 
ATTACAGCAG AAGGTTGTGC TGACTTGATG TCCGCATTTA ATTCAAACCC TTCACACCTG 
ACAGAGCTCA ATCTGAGTGT GAATGAACTA GGAGACTTAG GAGTAGAGAA GATCTGTCCA 
CTGCTGGAAA ATACACTGTG CAGATTGGAG AAATTAAATC TGTCAGACTG CAGTATCACT 
GGACAAAGTT ATGAAACTTT GGCTTCAGTT CTGAGATCAA ACTTGTATCT AAAAGAGCTA 
GATCTCAGAG GAAATGATCC TGGAAAATCA GGAGTGAAAA AGTTCATTGA TTTCCTGCAG 
GAGGGATCAT GTAAATTACA GATTATCCGG TTTCTGAAGA GTCCTGATGC AGAGGAAGCA 
TGCGAGTATC TCACTAAAGA TTTGGTCATA AATCCATTAT TACTGACAGA ACTGGATCTA 
AGTAAAGTTA AATTAGGACA TCTGGATGGA GAGAAATTCT CTGCTTTTTT GATGGACTCG 
CATAATAAAG TGGAGAAAAT AAAGCTGAAT AACTGTAAGC TGACAGAGAA AAGCTGTTCA 
GTTCTAGCTG CTGTTCTCTC CTCCAAAACC ATCCTGAAAG AGATGAACCT GAACAACAGT 
CGTCTGCTGG ACTCAGGAGT CAGAAAGATC TGTGAGGGAC TGAAGAATCC TGTGTGTGAG 
CTGAAGATAC TCAATCTTTC AGACTGCAGT ATCAGTGAAG AAGGTTATAA AGCTCTGGCT 
TCAGCTCTGA GATCAAACCC TTCACACCTG ATAGAGCTGG ATCTCAGAGG AAATGATCCT 
GGACAATCAG GAGTGAAGGA GCTCTTTAAT GTCCTACAGG ATCCTCAGTT TAAAACAAAA 
ATTAGGTTAT TGAGTCCAGC TGCTGATGAA GCCTGTTCGT TTGTGGCTGT ACATGTGGGT 
GAAAACCCGT TACTCCTGAA AGAACTGAAT CTGAGTAATT ATGAACTAGG AGACCCACGA 
GTGAATCAAC TCTCTGCTCT ACTGCAGGAT AAACACTGTA AACTCAACAC ACTCATGCTC 
AGTAATTGTG GTCTAACAGA GGAAAGCTGT TCAGCTCTTG CTTCAGTTCT CAGATCAGAC 
TCCTGCAGTC TGAAGGATCT TGACCTGAGC AATAATAATC TGCAGAATTC AGGAGTGAAG 
CGGCTCTCTG ATGGACTGAA GGACAACTGT AAATTGGAGA AACTCAGACT ATCAGACTGC 
AGTATCAGTG AAGGAGGTTA TAAAGCTCTG GCTTCAGCTC TGAGATCAAA CCTTTCACAC 
CTGATAGAGC TGGATCTCAG AGGAAATGAT CCTGGACAAT CAGGAGTGAA GGAGCTCACT 
GACTTACTGC AGGATCCACA CTGTACACTG AAGACACTGA GGTTTCTTAG TTCTGCTGTA 
GAGGAAGTCT GTAAACATTT GTCTGATGTT CTCACTGGGG ACCCGTTAAT CCTGAAAGAA 
CTGAATCTGG GTGGATGTAA AATAGAAGAC AAAGTGAGTT ATCTTGCTAC TCTACTGCAG 
GATAAACACT GTCAGATCAG CACACTGGTG CTGAACAATA CCAGTATTAC AGAAGAGGGT 
TGTGCTAATC TGACTTCAGC AATTACGTTA AACCCATCAC ACCTGAGGGA ACTGAACCTA 
AGTGATAATA AACTAGGAGA CTCTGCAGTG AAAAACCTCA GTGATTTACT GATAAAGACA 
CAATTCAAGC TGGAGAAACT ACATTTGAGT AATTGCAGTA TTACAGAGGA ACAGTGTGTG 
ATCCTGACTT CAGCTCTGAA ATCAAACCCA TCACACCTGA GAGAACTGAA CCTCAGTGTG 
AATGAAATGG GAGACTCTGC AGTGAAAAAC TTAAGGGATT TTCTGATGAA GCCACAATGC 
AATCTGGAGA AACTACTTCT GCGTCAATGC AGTATTACAG AGAAGCAGTG TGAGATCCTG 
ACTTCAGCTC TGAAATCAAA CCCATCACAC CTGAGAGAAC TGGACCTCAG TAGAAATGAA 
ATAAACAACA CAGGAGTAAA TCTTTTATCT GAAGTACTGA AGGATTCACA CTGTAAACTG 
GAGAGATTGA GTCTAATTGA ATGTGGCATC ACAGATGTTT CCTCTTTTGC TCACAATTTG 
ATGAACACAG AAACCCTGAA AGAGCTTGAT CTTAGAAACA ATTCTATATT AATTTCAAGG 
CGGAAACTGT TATATGTGCT ATCAGACTCT AACTGTAAAC TGAGCCTAGA CAAGGGTAAC 
TTGCATCAAC GTTTTGCTGA ATTATTTTTT GATAATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SI:CH211-213A13.9 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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