SI:CH211-278P9.4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:CH211-278P9.4
Entrez Gene ID	795914
Full Name	si:ch211-278p9.4
General protein information	Preferred Names si:ch211-278p9.4 Names NACHT, LRR and PYD domains-containing protein 12 NACHT, LRR and PYD domains-containing protein 3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297169.3	XP_009295444.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_009297172.2	XP_009295447.1	NACHT, LRR and PYD domains-containing protein 3 isoform X3
XM_009297169.2	XP_009295444.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_009297172.3	XP_009295447.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
XM_009297170.2	XP_009295445.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2
XM_009297173.3	XP_009295448.1	NACHT, LRR and PYD domains-containing protein 12 isoform X3
XM_009297173.2	XP_009295448.1	NACHT, LRR and PYD domains-containing protein 12 isoform X4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565414	XP_693785.6
Danio rerio (zebrafish)	LOC558375	XP_686669.1
Danio rerio (zebrafish)	ftr29	XP_003198034.1
Danio rerio (zebrafish)	ftr82	NP_001068571.1
Danio rerio (zebrafish)	LOC560422	XP_688915.1
Danio rerio (zebrafish)	LOC100000832	XP_001340954.2
Danio rerio (zebrafish)	LOC100006636	XP_005172385.1
Danio rerio (zebrafish)	ftr73	XP_005157866.1
Danio rerio (zebrafish)	ftr11	XP_003197971.1
Danio rerio (zebrafish)	LOC101882560	XP_005163612.1
Danio rerio (zebrafish)	ftr67	NP_001034808.2
Danio rerio (zebrafish)	ftr34	XP_005163669.1
Danio rerio (zebrafish)	ftr37	XP_696445.3
Danio rerio (zebrafish)	ftr30	XP_003198033.2
Danio rerio (zebrafish)	btr22	XP_005173784.1
Danio rerio (zebrafish)	btr18	XP_005173787.1
Danio rerio (zebrafish)	btr32	XP_003201305.1
Danio rerio (zebrafish)	btr07	XP_001923237.4
Danio rerio (zebrafish)	LOC100001589	XP_001341559.3
Danio rerio (zebrafish)	ftr79	XP_697299.2
Danio rerio (zebrafish)	ftr83	XP_688583.3
Danio rerio (zebrafish)	LOC100333853	XP_002661845.3
Danio rerio (zebrafish)	si:dkeyp-123a12.2	XP_691204.4
Danio rerio (zebrafish)	LOC100007791	XP_001346158.5
Danio rerio (zebrafish)	si:ch211-250k18.8	XP_698776.5
Danio rerio (zebrafish)	zgc:174944	NP_001108057.1
Danio rerio (zebrafish)	LOC566957	XP_005174040.1
Danio rerio (zebrafish)	LOC100150225	XP_001921257.4
Danio rerio (zebrafish)	LOC100334101	XP_003197833.2
Danio rerio (zebrafish)	LOC100334843	XP_002665168.2
Danio rerio (zebrafish)	si:ch73-24k9.2	XP_002665507.3
Danio rerio (zebrafish)	si:ch211-196h16.5	XP_690098.5
Danio rerio (zebrafish)	si:ch211-194e1.7	XP_005167387.1
Danio rerio (zebrafish)	LOC793291	XP_001331884.5
Danio rerio (zebrafish)	LOC100004711	XP_001340175.3
Danio rerio (zebrafish)	LOC100534746	XP_005161332.1
Danio rerio (zebrafish)	LOC100005625	XP_001339269.4
Danio rerio (zebrafish)	LOC100003765	XP_001343238.2
Danio rerio (zebrafish)	LOC101883156	XP_005160435.1
Danio rerio (zebrafish)	si:ch211-237a4.2	XP_003198575.2
Danio rerio (zebrafish)	zgc:194906	NP_001124145.1
Danio rerio (zebrafish)	LOC100536980	XP_003201699.2
Danio rerio (zebrafish)	si:ch211-15j1.3	XP_002660683.2
Danio rerio (zebrafish)	si:ch211-233g6.6	XP_002660707.3
Danio rerio (zebrafish)	LOC100331097	XP_002661460.3
Danio rerio (zebrafish)	LOC101885873	XP_005161560.1
Danio rerio (zebrafish)	LOC101883187	XP_005174457.1
Danio rerio (zebrafish)	LOC101882512	XP_005171750.1
Danio rerio (zebrafish)	LOC100329306	XP_005168148.1
Danio rerio (zebrafish)	LOC100535915	XP_005161831.1
Danio rerio (zebrafish)	si:ch211-114l13.1	XP_002660672.3
Danio rerio (zebrafish)	LOC101885187	XP_005165034.1
Danio rerio (zebrafish)	si:rp71-7l19.2	XP_003198343.2
Danio rerio (zebrafish)	LOC100331680	XP_002667178.3
Danio rerio (zebrafish)	LOC100005516	XP_005171758.1
Danio rerio (zebrafish)	LOC101883084	XP_005174455.1
Danio rerio (zebrafish)	LOC101883426	XP_005174069.1
Danio rerio (zebrafish)	LOC100150280	XP_005174567.1
Danio rerio (zebrafish)	LOC101884637	XP_005168127.1
Danio rerio (zebrafish)	LOC100329543	XP_005167374.1
Danio rerio (zebrafish)	LOC101882306	XP_005171748.1
Danio rerio (zebrafish)	LOC566508	XP_694871.6
Danio rerio (zebrafish)	LOC101884187	XP_005157862.1
Danio rerio (zebrafish)	ftr55	XP_003200609.2
Danio rerio (zebrafish)	LOC100330813	XP_002660428.2
Danio rerio (zebrafish)	ftr49	XP_003198077.1
Danio rerio (zebrafish)	LOC100535633	XP_003198959.1
Danio rerio (zebrafish)	btr04	XP_003198956.2
Danio rerio (zebrafish)	btr09	NP_001124100.1
Danio rerio (zebrafish)	LOC558675	XP_687012.2
Danio rerio (zebrafish)	ftr76	XP_005157864.1
Danio rerio (zebrafish)	ftr16	XP_690796.5
Danio rerio (zebrafish)	LOC561829	XP_690320.2
Danio rerio (zebrafish)	ftr03	XP_002660930.2
Danio rerio (zebrafish)	ftr06	XP_001337856.1
Danio rerio (zebrafish)	LOC570185	XP_698724.1
Danio rerio (zebrafish)	LOC797531	XP_001338000.2
Danio rerio (zebrafish)	ftr02	XP_003197969.1
Danio rerio (zebrafish)	LOC571442	XP_700123.5
Danio rerio (zebrafish)	ftr19	XP_002660960.3
Danio rerio (zebrafish)	LOC101882488	XP_005163611.1
Danio rerio (zebrafish)	ftr39p	XP_002661035.2
Danio rerio (zebrafish)	zgc:136302	XP_005170221.1
Danio rerio (zebrafish)	ftr58	XP_686163.5
Danio rerio (zebrafish)	LOC100537283	XP_003198007.2
Danio rerio (zebrafish)	ftr64	XP_002661885.1
Danio rerio (zebrafish)	btr31	XP_005170663.1
Danio rerio (zebrafish)	LOC100006950	XP_001345543.5
Danio rerio (zebrafish)	ftr28l	XP_005163705.1
Danio rerio (zebrafish)	LOC100333325	XP_002667878.3
Danio rerio (zebrafish)	si:ch211-121a2.3	XP_698105.5
Danio rerio (zebrafish)	LOC799802	XP_001336792.2
Danio rerio (zebrafish)	LOC555438	XP_683030.6
Danio rerio (zebrafish)	LOC568716	XP_003201051.2
Danio rerio (zebrafish)	LOC568664	XP_003201050.2
Danio rerio (zebrafish)	si:ch73-286h23.3	XP_001346239.5
Danio rerio (zebrafish)	LOC100006645	XP_001337665.3
Danio rerio (zebrafish)	wu:fb15h11	XP_697949.6
Danio rerio (zebrafish)	si:ch211-191a16.2	XP_001338550.5
Danio rerio (zebrafish)	si:ch211-181d7.1	XP_001919795.3
Danio rerio (zebrafish)	LOC795182	XP_001332957.4
Danio rerio (zebrafish)	LOC100329836	XP_002664746.3
Danio rerio (zebrafish)	LOC100334547	XP_002664742.2
Danio rerio (zebrafish)	si:dkey-156m2.3	XP_002664795.3
Danio rerio (zebrafish)	si:ch211-213a13.6	XP_697815.6
Danio rerio (zebrafish)	si:dkey-13n15.11	XP_005165261.1
Danio rerio (zebrafish)	si:zfos-364h11.1	XP_002665163.2
Danio rerio (zebrafish)	LOC100002266	XP_005158672.1
Danio rerio (zebrafish)	si:ch211-278p9.3	XP_002665167.3
Danio rerio (zebrafish)	zgc:136791	XP_005160238.1
Danio rerio (zebrafish)	LOC796214	XP_005161889.1
Danio rerio (zebrafish)	LOC100536939	XP_003200134.2
Danio rerio (zebrafish)	zgc:174178	NP_001107787.1
Danio rerio (zebrafish)	LOC101882503	XP_005168090.1
Danio rerio (zebrafish)	si:ch211-114l13.4	XP_002660681.1
Danio rerio (zebrafish)	si:ch211-15j1.5	XP_002660682.2
Danio rerio (zebrafish)	LOC100334715	XP_005171097.1
Danio rerio (zebrafish)	si:ch211-15j1.1	XP_001920778.3
Danio rerio (zebrafish)	si:dkey-222h21.8	XP_002660686.2
Danio rerio (zebrafish)	si:dkey-222h21.5	XP_002660689.1
Danio rerio (zebrafish)	LOC101882744	XP_005168242.1
Danio rerio (zebrafish)	LOC100329650	XP_002661801.3
Danio rerio (zebrafish)	si:ch211-255g12.8	XP_694303.5
Danio rerio (zebrafish)	LOC100331576	XP_002661628.3
Danio rerio (zebrafish)	LOC101883760	XP_005164630.1
Danio rerio (zebrafish)	LOC101886147	XP_005167368.1
Danio rerio (zebrafish)	LOC101886579	XP_005161565.1
Danio rerio (zebrafish)	LOC100333957	XP_005171081.1
Danio rerio (zebrafish)	LOC101884123	XP_005171093.1
Danio rerio (zebrafish)	LOC101884874	XP_005171101.1
Danio rerio (zebrafish)	LOC100333264	XP_002660697.3
Danio rerio (zebrafish)	LOC100330744	XP_002660427.3
Danio rerio (zebrafish)	ftr50	XP_005164403.1
Danio rerio (zebrafish)	LOC793898	XP_001920428.2
Danio rerio (zebrafish)	LOC791496	XP_003198035.2
Danio rerio (zebrafish)	LOC565787	XP_694149.5
Danio rerio (zebrafish)	LOC570079	XP_698604.5
Danio rerio (zebrafish)	LOC570163	XP_003197979.1
Danio rerio (zebrafish)	btr16	XP_005157512.1
Danio rerio (zebrafish)	LOC570251	XP_698802.4
Danio rerio (zebrafish)	ftr15	XP_700104.3
Danio rerio (zebrafish)	ftr26	XP_005163617.1
Danio rerio (zebrafish)	ftr65	XP_003198031.2
Danio rerio (zebrafish)	zgc:173666	XP_003198037.1
Danio rerio (zebrafish)	ftr61	XP_003201315.1
Danio rerio (zebrafish)	btr20	XP_003200561.1
Danio rerio (zebrafish)	zgc:173581	XP_005173811.1
Danio rerio (zebrafish)	btr06	NP_001120951.1
Danio rerio (zebrafish)	LOC101886307	XP_005163673.1
Danio rerio (zebrafish)	LOC101884427	XP_005159614.1
Danio rerio (zebrafish)	LOC100002745	XP_005164415.1
Danio rerio (zebrafish)	LOC101885734	XP_005161559.1
Danio rerio (zebrafish)	si:dkey-151g22.1	NP_001137518.1
Danio rerio (zebrafish)	si:dkey-121n8.4	XP_001335920.4
Danio rerio (zebrafish)	LOC100330073	XP_002661806.3
Danio rerio (zebrafish)	si:dkey-7i4.1	XP_005169721.1
Danio rerio (zebrafish)	LOC100001465	XP_005166707.1
Danio rerio (zebrafish)	LOC100005483	XP_001339130.3
Danio rerio (zebrafish)	LOC562679	XP_691146.5
Danio rerio (zebrafish)	LOC100333840	XP_005169858.1
Danio rerio (zebrafish)	LOC100536924	XP_003200567.2
Danio rerio (zebrafish)	LOC559196	XP_687605.5
Danio rerio (zebrafish)	LOC795109	XP_001335224.5
Danio rerio (zebrafish)	si:ch211-187g4.1	XP_698878.6
Danio rerio (zebrafish)	LOC100538217	XP_005169783.1
Danio rerio (zebrafish)	LOC571651	XP_700351.5
Danio rerio (zebrafish)	si:ch211-271g18.1	XP_002661463.3
Danio rerio (zebrafish)	LOC565381	XP_693750.6
Danio rerio (zebrafish)	si:ch211-238e22.7	XP_005168160.1
Danio rerio (zebrafish)	LOC100333747	XP_002661530.3
Danio rerio (zebrafish)	LOC100331072	XP_002661905.3
Danio rerio (zebrafish)	si:ch73-236c18.7	XP_002660699.3
Danio rerio (zebrafish)	si:dkey-222h21.6	XP_002660688.2
Danio rerio (zebrafish)	si:ch211-233g6.3	XP_002660709.3
Danio rerio (zebrafish)	LOC100329354	XP_002661501.3
Danio rerio (zebrafish)	LOC100006280	XP_001920132.4
Danio rerio (zebrafish)	LOC101886178	XP_005164994.1
Danio rerio (zebrafish)	LOC101883660	XP_005168155.1
Danio rerio (zebrafish)	LOC101883625	XP_005170693.1
Danio rerio (zebrafish)	LOC100150791	XP_005165031.1
Danio rerio (zebrafish)	LOC101883234	XP_005160282.1
Danio rerio (zebrafish)	LOC101885938	XP_005173831.1
Danio rerio (zebrafish)	LOC101885252	XP_005168541.1
Danio rerio (zebrafish)	trpm2	XP_003198602.2
Danio rerio (zebrafish)	btr12	NP_001070087.1
Danio rerio (zebrafish)	btr02	NP_001070626.2
Danio rerio (zebrafish)	ftr72	NP_001007388.2
Danio rerio (zebrafish)	LOC101883527	XP_005170222.1
Danio rerio (zebrafish)	trim25l	XP_005172141.1
Danio rerio (zebrafish)	LOC100149991	XP_003200554.1
Danio rerio (zebrafish)	LOC101883221	XP_005171452.1
Danio rerio (zebrafish)	ftr56	XP_003200605.1
Danio rerio (zebrafish)	LOC100334710	XP_002665516.2
Danio rerio (zebrafish)	btr25	XP_005173808.1
Danio rerio (zebrafish)	ftr24	XP_003197988.1
Danio rerio (zebrafish)	LOC100536343	XP_003197990.1
Danio rerio (zebrafish)	LOC100537128	XP_003200077.2
Danio rerio (zebrafish)	LOC100536410	XP_003200565.2
Danio rerio (zebrafish)	btr21	XP_003200564.2
Danio rerio (zebrafish)	ftr84	XP_005165427.1
Danio rerio (zebrafish)	si:ch211-150h8.3	XP_003198032.1
Danio rerio (zebrafish)	ftr43	XP_005164401.1
Danio rerio (zebrafish)	LOC571488	XP_700178.4
Danio rerio (zebrafish)	ftr41	XP_695494.5
Danio rerio (zebrafish)	ftr59	XP_686796.2
Danio rerio (zebrafish)	ftr51	XP_691169.5
Danio rerio (zebrafish)	ftr14l	XP_003199766.1
Danio rerio (zebrafish)	si:ch211-214b16.3	XP_005164324.1
Danio rerio (zebrafish)	LOC100331532	XP_003201602.2
Danio rerio (zebrafish)	LOC100329480	XP_002663890.3
Danio rerio (zebrafish)	si:dkey-222h21.2	XP_001334961.4
Danio rerio (zebrafish)	LOC100006564	NP_001119957.1
Danio rerio (zebrafish)	LOC100333746	XP_002661485.3
Danio rerio (zebrafish)	LOC100329448	XP_002667651.3
Danio rerio (zebrafish)	si:ch211-114l13.10	XP_696367.5
Danio rerio (zebrafish)	LOC100331159	XP_005169797.1
Danio rerio (zebrafish)	si:ch211-213a13.9	XP_003201049.2
Danio rerio (zebrafish)	si:dkey-253d23.10	XP_683789.6
Danio rerio (zebrafish)	si:ch211-278p9.4	XP_001336197.5
Danio rerio (zebrafish)	LOC566782	XP_695160.6
Danio rerio (zebrafish)	LOC558818	NP_001038341.1
Danio rerio (zebrafish)	si:ch211-149a19.3	XP_005170041.1
Danio rerio (zebrafish)	si:dkey-222h21.9	XP_002660685.1
Danio rerio (zebrafish)	LOC796962	XP_001923433.2
Danio rerio (zebrafish)	LOC100536895	XP_003200133.2
Danio rerio (zebrafish)	LOC100537076	XP_003200135.2
Danio rerio (zebrafish)	LOC100329927	XP_003200136.2
Danio rerio (zebrafish)	si:ch211-233m11.1	XP_687146.4
Danio rerio (zebrafish)	LOC100535360	XP_003200157.2
Danio rerio (zebrafish)	LOC101884992	XP_005174036.1
Danio rerio (zebrafish)	LOC794214	XP_001334125.1
Danio rerio (zebrafish)	LOC100537399	XP_003198502.2
Danio rerio (zebrafish)	LOC100007745	XP_001346125.5
Danio rerio (zebrafish)	si:dkey-126g1.7	XP_005162400.1
Danio rerio (zebrafish)	LOC101882643	XP_005171752.1
Danio rerio (zebrafish)	LOC100001765	XP_001341704.4
Danio rerio (zebrafish)	si:dkey-82i20.1	XP_002666374.3
Danio rerio (zebrafish)	LOC100333748	XP_002661966.3
Danio rerio (zebrafish)	LOC565296	XP_003198495.2
Danio rerio (zebrafish)	LOC100535428	XP_005174493.1
Danio rerio (zebrafish)	LOC100334677	XP_002661542.3
Danio rerio (zebrafish)	LOC100329558	XP_002661549.3
Danio rerio (zebrafish)	si:dkeyp-4c4.1	NP_001094426.1
Danio rerio (zebrafish)	LOC101885760	XP_005164976.1
Danio rerio (zebrafish)	LOC101884884	XP_005157350.1
Danio rerio (zebrafish)	LOC101885212	XP_005158663.1
Danio rerio (zebrafish)	LOC101885461	XP_005168195.1
Danio rerio (zebrafish)	LOC560232	NP_001116726.1
Danio rerio (zebrafish)	LOC101885169	XP_005174039.1
Danio rerio (zebrafish)	LOC101883611	XP_005174070.1

Gene Ontology

The following SI:CH211-278P9.4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:CH211-278P9.4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa47021	XM_009297169.3 Latest version!	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa47022	XM_009297170.2 Latest version!	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa47024	XM_009297172.3 Latest version!	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa47025	XM_009297173.3 Latest version!	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
ODa47021	XM_009297169.2	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa47024	XM_009297172.2	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa47025	XM_009297173.2	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:CH211-278P9.4 ( XM_009297169.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295444.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa47021
Clone ID Related Accession (Same CDS sequence)	XM_009297169.3 , XM_009297169.2
Accession Version	XM_009297169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTTAATGG TAGCAATATC AGAGAGAAAG ATTGTGCTGC TTTCATTTCA 
TCTCTTCTAT CAAACCCGTT TCATCTGAGG GAACTCAGTT TGAGCTGGAA CAAACCTGGC 
GACTCTGGAG TCAAGCTTTT GTCAGATCTT CTGAAAGATT CTCGCTGTAA ACTGGAAAAA 
CTGGAGCTGG AGTGCTGCAG TATTATAGAG GAAGGCTGTG CTGCTCTGGC TTTAGCACTT 
GTATCAAACC CATCTCACCT GAGAGAACTC AATCTAAACA ATAATGAACC AGGAGACCTA 
GGAGTGAACC TTTTGTCTTT TCTTCTGAAA AATCCACAAT GTAAACTAGA GAAACTGGAG 
CTGGAATACT GCTGCATAGG TGCAAAAAGT TGTGCTTCTC TGGCTTCTGC TTTTGTATCC 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AACTGGAACA CACCAGAATA TCCTGCAGTG 
AAGCTTCTGT CTGATTATCT GAAGGATCCA CACTGTAAAC TGGAGAAACT ACAGTTGTAT 
AACTGCTGTA TTGGTGAGGA AGGCTGTGCT GCACTGGCTT TAGCTCTGAT ATCAAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAACAAT AATGAACCAG GCGATTCAGG AGTGAAGCTT 
CTCTCTGATC TGCTGAAGGA TCCGCGCTGT AAACTGGAGA AACTACAGCT GGAATACTGC 
TGTATCGGTG AGGAAGGTTG TGCTGCTCTG GTTTCAGGTC TGAAATCGAA CCCGTCACAC 
CTGAGGGAAC TCAATCTGAA CAGAAACGAA CCTGGAAACT TAGGTGCAAA GATGCTGTCA 
GATCTCCTGA AGGATCCGCT GTTTGAACTT GAGAAACTAG AGCTCGATGA CTGCAGTATT 
GAAAACAATG GTTTTGCTGA TCTAGTTTCA GGCCTGAAAT CAAACCCATC AAACATCAGA 
GAGCTCAATC TGAACTCTAA TAAATTCATC TTCTCTGGAT TAAATCTACT CTGTGACCTG 
CTGAGAGATC AGCAGTGTAA ACTGCGGAAG CTACAGCTGG AGTGCTGCAA TATAGATGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCACTG ATATCTAACC CGTCTCATCT GAGAGAACTC 
AATCTGAATT ACAACAATCC AGGAGGCCTT GGAGTGAAGC TTCTGTCTGT TCTTCTGAAG 
AATCCACACT GTAAACTGGA GAAACTGGAG TTGTCTCATT GCAGTATTTC AGAGAATTAC 
CTTGCTGCTC TAAATTCAGC TCTGAGTTCA AACCCAGCAC ACTTGAGGGA ACTCAATTTG 
AACTACAATG AGAACACTAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295444.1
CDS	181..4347
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLNGSNIREKDCAAFISSLLSNPFHLRELSLSWNKPGDSGVKLLSDLLKDSRCKLEKLELECCSIIEEGCAALALALVSNPSHLRELNLNNNEPGDLGVNLLSFLLKNPQCKLEKLELEYCCIGAKSCASLASAFVSNPSHLRELNLNWNTPEYPAVKLLSDYLKDPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297169.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297169.3

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTTAATGG TAGCAATATC AGAGAGAAAG ATTGTGCTGC TTTCATTTCA 
TCTCTTCTAT CAAACCCGTT TCATCTGAGG GAACTCAGTT TGAGCTGGAA CAAACCTGGC 
GACTCTGGAG TCAAGCTTTT GTCAGATCTT CTGAAAGATT CTCGCTGTAA ACTGGAAAAA 
CTGGAGCTGG AGTGCTGCAG TATTATAGAG GAAGGCTGTG CTGCTCTGGC TTTAGCACTT 
GTATCAAACC CATCTCACCT GAGAGAACTC AATCTAAACA ATAATGAACC AGGAGACCTA 
GGAGTGAACC TTTTGTCTTT TCTTCTGAAA AATCCACAAT GTAAACTAGA GAAACTGGAG 
CTGGAATACT GCTGCATAGG TGCAAAAAGT TGTGCTTCTC TGGCTTCTGC TTTTGTATCC 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AACTGGAACA CACCAGAATA TCCTGCAGTG 
AAGCTTCTGT CTGATTATCT GAAGGATCCA CACTGTAAAC TGGAGAAACT ACAGTTGTAT 
AACTGCTGTA TTGGTGAGGA AGGCTGTGCT GCACTGGCTT TAGCTCTGAT ATCAAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAACAAT AATGAACCAG GCGATTCAGG AGTGAAGCTT 
CTCTCTGATC TGCTGAAGGA TCCGCGCTGT AAACTGGAGA AACTACAGCT GGAATACTGC 
TGTATCGGTG AGGAAGGTTG TGCTGCTCTG GTTTCAGGTC TGAAATCGAA CCCGTCACAC 
CTGAGGGAAC TCAATCTGAA CAGAAACGAA CCTGGAAACT TAGGTGCAAA GATGCTGTCA 
GATCTCCTGA AGGATCCGCT GTTTGAACTT GAGAAACTAG AGCTCGATGA CTGCAGTATT 
GAAAACAATG GTTTTGCTGA TCTAGTTTCA GGCCTGAAAT CAAACCCATC AAACATCAGA 
GAGCTCAATC TGAACTCTAA TAAATTCATC TTCTCTGGAT TAAATCTACT CTGTGACCTG 
CTGAGAGATC AGCAGTGTAA ACTGCGGAAG CTACAGCTGG AGTGCTGCAA TATAGATGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCACTG ATATCTAACC CGTCTCATCT GAGAGAACTC 
AATCTGAATT ACAACAATCC AGGAGGCCTT GGAGTGAAGC TTCTGTCTGT TCTTCTGAAG 
AATCCACACT GTAAACTGGA GAAACTGGAG TTGTCTCATT GCAGTATTTC AGAGAATTAC 
CTTGCTGCTC TAAATTCAGC TCTGAGTTCA AACCCAGCAC ACTTGAGGGA ACTCAATTTG 
AACTACAATG AGAACACTAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297169.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295444.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.

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CloneID	ODa47021
Clone ID Related Accession (Same CDS sequence)	XM_009297169.3 , XM_009297169.2
Accession Version	XM_009297169.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTTAATGG TAGCAATATC AGAGAGAAAG ATTGTGCTGC TTTCATTTCA 
TCTCTTCTAT CAAACCCGTT TCATCTGAGG GAACTCAGTT TGAGCTGGAA CAAACCTGGC 
GACTCTGGAG TCAAGCTTTT GTCAGATCTT CTGAAAGATT CTCGCTGTAA ACTGGAAAAA 
CTGGAGCTGG AGTGCTGCAG TATTATAGAG GAAGGCTGTG CTGCTCTGGC TTTAGCACTT 
GTATCAAACC CATCTCACCT GAGAGAACTC AATCTAAACA ATAATGAACC AGGAGACCTA 
GGAGTGAACC TTTTGTCTTT TCTTCTGAAA AATCCACAAT GTAAACTAGA GAAACTGGAG 
CTGGAATACT GCTGCATAGG TGCAAAAAGT TGTGCTTCTC TGGCTTCTGC TTTTGTATCC 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AACTGGAACA CACCAGAATA TCCTGCAGTG 
AAGCTTCTGT CTGATTATCT GAAGGATCCA CACTGTAAAC TGGAGAAACT ACAGTTGTAT 
AACTGCTGTA TTGGTGAGGA AGGCTGTGCT GCACTGGCTT TAGCTCTGAT ATCAAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAACAAT AATGAACCAG GCGATTCAGG AGTGAAGCTT 
CTCTCTGATC TGCTGAAGGA TCCGCGCTGT AAACTGGAGA AACTACAGCT GGAATACTGC 
TGTATCGGTG AGGAAGGTTG TGCTGCTCTG GTTTCAGGTC TGAAATCGAA CCCGTCACAC 
CTGAGGGAAC TCAATCTGAA CAGAAACGAA CCTGGAAACT TAGGTGCAAA GATGCTGTCA 
GATCTCCTGA AGGATCCGCT GTTTGAACTT GAGAAACTAG AGCTCGATGA CTGCAGTATT 
GAAAACAATG GTTTTGCTGA TCTAGTTTCA GGCCTGAAAT CAAACCCATC AAACATCAGA 
GAGCTCAATC TGAACTCTAA TAAATTCATC TTCTCTGGAT TAAATCTACT CTGTGACCTG 
CTGAGAGATC AGCAGTGTAA ACTGCGGAAG CTACAGCTGG AGTGCTGCAA TATAGATGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCACTG ATATCTAACC CGTCTCATCT GAGAGAACTC 
AATCTGAATT ACAACAATCC AGGAGGCCTT GGAGTGAAGC TTCTGTCTGT TCTTCTGAAG 
AATCCACACT GTAAACTGGA GAAACTGGAG TTGTCTCATT GCAGTATTTC AGAGAATTAC 
CTTGCTGCTC TAAATTCAGC TCTGAGTTCA AACCCAGCAC ACTTGAGGGA ACTCAATTTG 
AACTACAATG AGAACACTAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295444.1
CDS	193..4359
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLNGSNIREKDCAAFISSLLSNPFHLRELSLSWNKPGDSGVKLLSDLLKDSRCKLEKLELECCSIIEEGCAALALALVSNPSHLRELNLNNNEPGDLGVNLLSFLLKNPQCKLEKLELEYCCIGAKSCASLASAFVSNPSHLRELNLNWNTPEYPAVKLLSDYLKDPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297169.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297169.2

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTTAATGG TAGCAATATC AGAGAGAAAG ATTGTGCTGC TTTCATTTCA 
TCTCTTCTAT CAAACCCGTT TCATCTGAGG GAACTCAGTT TGAGCTGGAA CAAACCTGGC 
GACTCTGGAG TCAAGCTTTT GTCAGATCTT CTGAAAGATT CTCGCTGTAA ACTGGAAAAA 
CTGGAGCTGG AGTGCTGCAG TATTATAGAG GAAGGCTGTG CTGCTCTGGC TTTAGCACTT 
GTATCAAACC CATCTCACCT GAGAGAACTC AATCTAAACA ATAATGAACC AGGAGACCTA 
GGAGTGAACC TTTTGTCTTT TCTTCTGAAA AATCCACAAT GTAAACTAGA GAAACTGGAG 
CTGGAATACT GCTGCATAGG TGCAAAAAGT TGTGCTTCTC TGGCTTCTGC TTTTGTATCC 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AACTGGAACA CACCAGAATA TCCTGCAGTG 
AAGCTTCTGT CTGATTATCT GAAGGATCCA CACTGTAAAC TGGAGAAACT ACAGTTGTAT 
AACTGCTGTA TTGGTGAGGA AGGCTGTGCT GCACTGGCTT TAGCTCTGAT ATCAAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAACAAT AATGAACCAG GCGATTCAGG AGTGAAGCTT 
CTCTCTGATC TGCTGAAGGA TCCGCGCTGT AAACTGGAGA AACTACAGCT GGAATACTGC 
TGTATCGGTG AGGAAGGTTG TGCTGCTCTG GTTTCAGGTC TGAAATCGAA CCCGTCACAC 
CTGAGGGAAC TCAATCTGAA CAGAAACGAA CCTGGAAACT TAGGTGCAAA GATGCTGTCA 
GATCTCCTGA AGGATCCGCT GTTTGAACTT GAGAAACTAG AGCTCGATGA CTGCAGTATT 
GAAAACAATG GTTTTGCTGA TCTAGTTTCA GGCCTGAAAT CAAACCCATC AAACATCAGA 
GAGCTCAATC TGAACTCTAA TAAATTCATC TTCTCTGGAT TAAATCTACT CTGTGACCTG 
CTGAGAGATC AGCAGTGTAA ACTGCGGAAG CTACAGCTGG AGTGCTGCAA TATAGATGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCACTG ATATCTAACC CGTCTCATCT GAGAGAACTC 
AATCTGAATT ACAACAATCC AGGAGGCCTT GGAGTGAAGC TTCTGTCTGT TCTTCTGAAG 
AATCCACACT GTAAACTGGA GAAACTGGAG TTGTCTCATT GCAGTATTTC AGAGAATTAC 
CTTGCTGCTC TAAATTCAGC TCTGAGTTCA AACCCAGCAC ACTTGAGGGA ACTCAATTTG 
AACTACAATG AGAACACTAG ACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297170.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295445.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.

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CloneID	ODa47022
Clone ID Related Accession (Same CDS sequence)	XM_009297170.2
Accession Version	XM_009297170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCCTGAGTC CAGCTGTGTG TCCATGAAGA GCGACGCTTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCTAAACACA GTGACAACCA AAAGCGATCA 
CAGTCAAATA AAGGCCAGAA GCAGCTAGAC GCCATTTTCA AGGACCTTGA GGAAACAAAC 
ATAGATGAGA TGAGGAAAGT GTTGAGAAGA TTCAAGATGC TGCTTAGTCC AGATTACTCT 
GTGAGAGATG AGGAGGAGGA GGAAGGTCAA CGTCAAATAA ACGAGGCATT TCTGAAGATC 
ACTCTTCATG CCCTCCAGAA GATGAACCAG ACGGACCTCG CTAGCAAACT ACAAACCAAG 
TTAATCTCCG TGAGTCAACA ACAGCTCAAA TCCAGACTGA AGCAGAGATT TGAGAGATTT 
ACTGAAGGAG TCTCAAATCG AGGCAATACA AAACTTCTGA ATGACATCTA CACAGAGGTT 
TACATCACTG AGGGAGGGGC TGGAAACATT GACGATGAGC ACGAGGTGAG ACAGATCGAG 
ACCTCGTCGT CCAGATTTCC AGAGATACCG ATTAGATGTT GCGACATCTT CAAATCCTTA 
CTGGGACAGG ACAAAGCCAT CAGAACGGTT CTGACCAAAG GAGTAGCTGG AATCGGAAAA 
ACCGCTTCTG TGCAGAAGTT CATCCTAGAC TGGGCAGAAG GAAAAGCCAA TCAGGATGTT 
CACTTCATAT TTCCGCTTCC TTTCAGAGAC TTGAATTTGA TAAAGGAGAA ACGGATGAGT 
CTGATGGAGC TCCTTTGCTG TTTTTGCCCA GAACTAAAAC CGATAGACTA TAACAACTTC 
AAAACAGTCT TCATCTATGA TGGTCTGGAT GAGTGGCGCC TTCCTCTGGA TTTCCGGAAC 
AATGAGATTT TGTGGGATAT GAACAAAGTG GCCTCTGTGG ATGCACTGCT TACCAACCTC 
CTCCAGGGGA ACCTGCTTCC TTCTGCTGTC CACTGGATCA CCACACGACC AGCCGCAGCC 
TGTCAGATCC CAGCTGAGTT TATTGATCGG GTCACCGAGG TACGAGGGTT CAAAGATCTT 
CAGAGAGCAG AGTATTTCTG GAAAAGAATA AAAGATCAGA ATCTTGCCAA CAAGATCATC 
ACACACATTC AATCATCAAG AAGCCTTCAC ATCATGTGCC GCATCCCAGT GTTCTGCTGG 
ATCTCCGCCA CTGTTCTTGA GAAGATCTTT GGTGAAGCGA ATAGTGCAGA CATTCCCAAA 
ACTCTCACTC AAATGTTCAT ACACCTGCTG ATCGTTCAGA CCAATCTGAA GACCCAGAAA 
TATGACCGAG AATGTGATGT AAATCTTGAG AAGGCAAGAA AGAGCATCTT GTCACTTGCG 
AAACTGGCAT TTGAGCAACT GGAGAAAGGG AACCTGATCT TCTATGAAGA GGATTTGAAA 
CAGTGTGGCA TTGATGTCCG AGAAGCGTCC GTGTGCTCTG GAGTGTGTAC CCAGATCTTT 
CGGGAAGAGT TTGGGCTGCA GCTTCAAAAG GTGTTTAGCT TTGTACATCT GAGTGTTCAG 
GAGTTTCTCG CTGCCTTGTT TAAGTTTCTT TCCTTTGCTG AAGAAAACAA CAAGTCCTCA 
ATGACACAGT TTATGAAGAA CGAAGTGGAC AGGGCCTTGC AGAGTCCGAA CGGACACCTT 
GATCTCTTCT TACGTTTTCT TCTGGGTCTT TCACTGGACT CCAATCAGAC TCTCCTACAG 
GGATTATTCA CTCCGTTGGA TACCTCTCAC AGCAGAGACG AGACTATTGA GTATATAAAA 
AGTAAAATCA GAGAAAATCC GTCTCCGGAG AGATCCATCA ATCTGTTTTA CTGCCTAAAT 
GAGCTGAATG ATCATTCTCT GGTGCAGGAG GTCCAAACAT ATCTAACCAG CACACGCAGC 
CATCGCCTCT CTGGAGTGAA ACTGTCTCCG GCCCTGTGGT CAGCACTGGT GTTTGTGTTG 
CTGAACTCGA CAAAGGACCT GGACGAGTTT GAGCTCAGGC AATACGATTT TACAGAGGAG 
TGTCTTCTGA GGCTGCTACC GGTGGTCAAA GCTTCCAGAA AAGCAGATTT GAAAGGTGGT 
AATCTCACAG ATCAGATCTG TTCAGCTCTG GCCTCTGCTC TCAGCTCGAG CTCCTGCTGT 
CTGCGAGAAC TGGACCTCAG TGAAAACAGG CTCCAGCTTT CAGGAATACG ACTTTTCTCT 
GCAGGACTGA AGAGCCCTAA CTGTAAGCTG GAGATACTCA AGTTGAGCAA CTGCAGTATT 
AGAGAGGAAG GCTGTGCTTC TCTGGTTTCT GCTCTTCTCT CAAACCCGTC TCATCTGAGA 
GAACTCAACC TGAACTCCAA TCAACCAGGA TATTCAACAG TGAAGCTTTT GTCTGATCTT 
CTGGAGAATC CACACTGTAA ACTGGAGAAA CTACAGTTGT ATAACTGCTG TATTGGTGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCTCTG ATATCAAACC CGTCTCATCT GAGAGAACTC 
AATCTGAACA ATAATGAACC AGGCGATTCA GGAGTGAAGC TTCTCTCTGA TCTGCTGAAG 
GATCCGCGCT GTAAACTGGA GAAACTACAG CTTAATGGTA GCAATATCAG AGAGAAAGAT 
TGTGCTGCTT TCATTTCATC TCTTCTATCA AACCCGTTTC ATCTGAGGGA ACTCAGTTTG 
AGCTGGAACA AACCTGGCGA CTCTGGAGTC AAGCTTTTGT CAGATCTTCT GAAAGATTCT 
CGCTGTAAAC TGGAAAAACT GGAGCTGGAG TGCTGCAGTA TTATAGAGGA AGGCTGTGCT 
GCTCTGGCTT TAGCACTTGT ATCAAACCCA TCTCACCTGA GAGAACTCAA TCTAAACAAT 
AATGAACCAG GAGACCTAGG AGTGAACCTT TTGTCTTTTC TTCTGAAAAA TCCACAATGT 
AAACTAGAGA AACTGGAGCT GGAATACTGC TGCATAGGTG CAAAAAGTTG TGCTTCTCTG 
GCTTCTGCTT TTGTATCCAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CTGGAACACA 
CCAGAATATC CTGCAGTGAA GCTTCTGTCT GATTATCTGA AGGATCCACA CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295445.1
CDS	193..4257
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLNGSNIREKDCAAFISSLLSNPFHLRELSLSWNKPGDSGVKLLSDLLKDSRCKLEKLELECCSIIEEGCAALALALVSNPSHLRELNLNNNEPGDLGVNLLSFLLKNPQCKLEKLELEYCCIGAKSCASLASAFVSNPSHLRELNLNWNTPEYPAVKLLSDYLKDPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297170.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297170.2

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCCTGAGTC CAGCTGTGTG TCCATGAAGA GCGACGCTTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCTAAACACA GTGACAACCA AAAGCGATCA 
CAGTCAAATA AAGGCCAGAA GCAGCTAGAC GCCATTTTCA AGGACCTTGA GGAAACAAAC 
ATAGATGAGA TGAGGAAAGT GTTGAGAAGA TTCAAGATGC TGCTTAGTCC AGATTACTCT 
GTGAGAGATG AGGAGGAGGA GGAAGGTCAA CGTCAAATAA ACGAGGCATT TCTGAAGATC 
ACTCTTCATG CCCTCCAGAA GATGAACCAG ACGGACCTCG CTAGCAAACT ACAAACCAAG 
TTAATCTCCG TGAGTCAACA ACAGCTCAAA TCCAGACTGA AGCAGAGATT TGAGAGATTT 
ACTGAAGGAG TCTCAAATCG AGGCAATACA AAACTTCTGA ATGACATCTA CACAGAGGTT 
TACATCACTG AGGGAGGGGC TGGAAACATT GACGATGAGC ACGAGGTGAG ACAGATCGAG 
ACCTCGTCGT CCAGATTTCC AGAGATACCG ATTAGATGTT GCGACATCTT CAAATCCTTA 
CTGGGACAGG ACAAAGCCAT CAGAACGGTT CTGACCAAAG GAGTAGCTGG AATCGGAAAA 
ACCGCTTCTG TGCAGAAGTT CATCCTAGAC TGGGCAGAAG GAAAAGCCAA TCAGGATGTT 
CACTTCATAT TTCCGCTTCC TTTCAGAGAC TTGAATTTGA TAAAGGAGAA ACGGATGAGT 
CTGATGGAGC TCCTTTGCTG TTTTTGCCCA GAACTAAAAC CGATAGACTA TAACAACTTC 
AAAACAGTCT TCATCTATGA TGGTCTGGAT GAGTGGCGCC TTCCTCTGGA TTTCCGGAAC 
AATGAGATTT TGTGGGATAT GAACAAAGTG GCCTCTGTGG ATGCACTGCT TACCAACCTC 
CTCCAGGGGA ACCTGCTTCC TTCTGCTGTC CACTGGATCA CCACACGACC AGCCGCAGCC 
TGTCAGATCC CAGCTGAGTT TATTGATCGG GTCACCGAGG TACGAGGGTT CAAAGATCTT 
CAGAGAGCAG AGTATTTCTG GAAAAGAATA AAAGATCAGA ATCTTGCCAA CAAGATCATC 
ACACACATTC AATCATCAAG AAGCCTTCAC ATCATGTGCC GCATCCCAGT GTTCTGCTGG 
ATCTCCGCCA CTGTTCTTGA GAAGATCTTT GGTGAAGCGA ATAGTGCAGA CATTCCCAAA 
ACTCTCACTC AAATGTTCAT ACACCTGCTG ATCGTTCAGA CCAATCTGAA GACCCAGAAA 
TATGACCGAG AATGTGATGT AAATCTTGAG AAGGCAAGAA AGAGCATCTT GTCACTTGCG 
AAACTGGCAT TTGAGCAACT GGAGAAAGGG AACCTGATCT TCTATGAAGA GGATTTGAAA 
CAGTGTGGCA TTGATGTCCG AGAAGCGTCC GTGTGCTCTG GAGTGTGTAC CCAGATCTTT 
CGGGAAGAGT TTGGGCTGCA GCTTCAAAAG GTGTTTAGCT TTGTACATCT GAGTGTTCAG 
GAGTTTCTCG CTGCCTTGTT TAAGTTTCTT TCCTTTGCTG AAGAAAACAA CAAGTCCTCA 
ATGACACAGT TTATGAAGAA CGAAGTGGAC AGGGCCTTGC AGAGTCCGAA CGGACACCTT 
GATCTCTTCT TACGTTTTCT TCTGGGTCTT TCACTGGACT CCAATCAGAC TCTCCTACAG 
GGATTATTCA CTCCGTTGGA TACCTCTCAC AGCAGAGACG AGACTATTGA GTATATAAAA 
AGTAAAATCA GAGAAAATCC GTCTCCGGAG AGATCCATCA ATCTGTTTTA CTGCCTAAAT 
GAGCTGAATG ATCATTCTCT GGTGCAGGAG GTCCAAACAT ATCTAACCAG CACACGCAGC 
CATCGCCTCT CTGGAGTGAA ACTGTCTCCG GCCCTGTGGT CAGCACTGGT GTTTGTGTTG 
CTGAACTCGA CAAAGGACCT GGACGAGTTT GAGCTCAGGC AATACGATTT TACAGAGGAG 
TGTCTTCTGA GGCTGCTACC GGTGGTCAAA GCTTCCAGAA AAGCAGATTT GAAAGGTGGT 
AATCTCACAG ATCAGATCTG TTCAGCTCTG GCCTCTGCTC TCAGCTCGAG CTCCTGCTGT 
CTGCGAGAAC TGGACCTCAG TGAAAACAGG CTCCAGCTTT CAGGAATACG ACTTTTCTCT 
GCAGGACTGA AGAGCCCTAA CTGTAAGCTG GAGATACTCA AGTTGAGCAA CTGCAGTATT 
AGAGAGGAAG GCTGTGCTTC TCTGGTTTCT GCTCTTCTCT CAAACCCGTC TCATCTGAGA 
GAACTCAACC TGAACTCCAA TCAACCAGGA TATTCAACAG TGAAGCTTTT GTCTGATCTT 
CTGGAGAATC CACACTGTAA ACTGGAGAAA CTACAGTTGT ATAACTGCTG TATTGGTGAG 
GAAGGCTGTG CTGCTCTGGC TTTAGCTCTG ATATCAAACC CGTCTCATCT GAGAGAACTC 
AATCTGAACA ATAATGAACC AGGCGATTCA GGAGTGAAGC TTCTCTCTGA TCTGCTGAAG 
GATCCGCGCT GTAAACTGGA GAAACTACAG CTTAATGGTA GCAATATCAG AGAGAAAGAT 
TGTGCTGCTT TCATTTCATC TCTTCTATCA AACCCGTTTC ATCTGAGGGA ACTCAGTTTG 
AGCTGGAACA AACCTGGCGA CTCTGGAGTC AAGCTTTTGT CAGATCTTCT GAAAGATTCT 
CGCTGTAAAC TGGAAAAACT GGAGCTGGAG TGCTGCAGTA TTATAGAGGA AGGCTGTGCT 
GCTCTGGCTT TAGCACTTGT ATCAAACCCA TCTCACCTGA GAGAACTCAA TCTAAACAAT 
AATGAACCAG GAGACCTAGG AGTGAACCTT TTGTCTTTTC TTCTGAAAAA TCCACAATGT 
AAACTAGAGA AACTGGAGCT GGAATACTGC TGCATAGGTG CAAAAAGTTG TGCTTCTCTG 
GCTTCTGCTT TTGTATCCAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CTGGAACACA 
CCAGAATATC CTGCAGTGAA GCTTCTGTCT GATTATCTGA AGGATCCACA CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297172.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295447.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.

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CloneID	ODa47024
Clone ID Related Accession (Same CDS sequence)	XM_009297172.2 , XM_009297172.3
Accession Version	XM_009297172.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295447.1
CDS	193..3837
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297172.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297172.2

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297172.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295447.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47024
Clone ID Related Accession (Same CDS sequence)	XM_009297172.2 , XM_009297172.3
Accession Version	XM_009297172.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297172.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295447.1
CDS	181..3825
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297172.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297172.3

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGTTGTATAA CTGCTGTATT GGTGAGGAAG GCTGTGCTGC ACTGGCTTTA 
GCTCTGATAT CAAACCCGTC TCATCTGAGA GAACTCAATC TGAACAATAA TGAACCAGGC 
GATTCAGGAG TGAAGCTTCT CTCTGATCTG CTGAAGGATC CGCGCTGTAA ACTGGAGAAA 
CTACAGCTGG AATACTGCTG TATCGGTGAG GAAGGTTGTG CTGCTCTGGT TTCAGGTCTG 
AAATCGAACC CGTCACACCT GAGGGAACTC AATCTGAACA GAAACGAACC TGGAAACTTA 
GGTGCAAAGA TGCTGTCAGA TCTCCTGAAG GATCCGCTGT TTGAACTTGA GAAACTAGAG 
CTCGATGACT GCAGTATTGA AAACAATGGT TTTGCTGATC TAGTTTCAGG CCTGAAATCA 
AACCCATCAA ACATCAGAGA GCTCAATCTG AACTCTAATA AATTCATCTT CTCTGGATTA 
AATCTACTCT GTGACCTGCT GAGAGATCAG CAGTGTAAAC TGCGGAAGCT ACAGCTGGAG 
TGCTGCAATA TAGATGAGGA AGGCTGTGCT GCTCTGGCTT TAGCACTGAT ATCTAACCCG 
TCTCATCTGA GAGAACTCAA TCTGAATTAC AACAATCCAG GAGGCCTTGG AGTGAAGCTT 
CTGTCTGTTC TTCTGAAGAA TCCACACTGT AAACTGGAGA AACTGGAGTT GTCTCATTGC 
AGTATTTCAG AGAATTACCT TGCTGCTCTA AATTCAGCTC TGAGTTCAAA CCCAGCACAC 
TTGAGGGAAC TCAATTTGAA CTACAATGAG AACACTAGAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297173.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295448.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.

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CloneID	ODa47025
Clone ID Related Accession (Same CDS sequence)	XM_009297173.3 , XM_009297173.2
Accession Version	XM_009297173.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297173.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTGGAATA CTGCTGTATC GGTGAGGAAG GTTGTGCTGC TCTGGTTTCA 
GGTCTGAAAT CGAACCCGTC ACACCTGAGG GAACTCAATC TGAACAGAAA CGAACCTGGA 
AACTTAGGTG CAAAGATGCT GTCAGATCTC CTGAAGGATC CGCTGTTTGA ACTTGAGAAA 
CTAGAGCTCG ATGACTGCAG TATTGAAAAC AATGGTTTTG CTGATCTAGT TTCAGGCCTG 
AAATCAAACC CATCAAACAT CAGAGAGCTC AATCTGAACT CTAATAAATT CATCTTCTCT 
GGATTAAATC TACTCTGTGA CCTGCTGAGA GATCAGCAGT GTAAACTGCG GAAGCTACAG 
CTGGAGTGCT GCAATATAGA TGAGGAAGGC TGTGCTGCTC TGGCTTTAGC ACTGATATCT 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AATTACAACA ATCCAGGAGG CCTTGGAGTG 
AAGCTTCTGT CTGTTCTTCT GAAGAATCCA CACTGTAAAC TGGAGAAACT GGAGTTGTCT 
CATTGCAGTA TTTCAGAGAA TTACCTTGCT GCTCTAAATT CAGCTCTGAG TTCAAACCCA 
GCACACTTGA GGGAACTCAA TTTGAACTAC AATGAGAACA CTAGACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295448.1
CDS	181..3651
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297173.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297173.3

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTGGAATA CTGCTGTATC GGTGAGGAAG GTTGTGCTGC TCTGGTTTCA 
GGTCTGAAAT CGAACCCGTC ACACCTGAGG GAACTCAATC TGAACAGAAA CGAACCTGGA 
AACTTAGGTG CAAAGATGCT GTCAGATCTC CTGAAGGATC CGCTGTTTGA ACTTGAGAAA 
CTAGAGCTCG ATGACTGCAG TATTGAAAAC AATGGTTTTG CTGATCTAGT TTCAGGCCTG 
AAATCAAACC CATCAAACAT CAGAGAGCTC AATCTGAACT CTAATAAATT CATCTTCTCT 
GGATTAAATC TACTCTGTGA CCTGCTGAGA GATCAGCAGT GTAAACTGCG GAAGCTACAG 
CTGGAGTGCT GCAATATAGA TGAGGAAGGC TGTGCTGCTC TGGCTTTAGC ACTGATATCT 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AATTACAACA ATCCAGGAGG CCTTGGAGTG 
AAGCTTCTGT CTGTTCTTCT GAAGAATCCA CACTGTAAAC TGGAGAAACT GGAGTTGTCT 
CATTGCAGTA TTTCAGAGAA TTACCTTGCT GCTCTAAATT CAGCTCTGAG TTCAAACCCA 
GCACACTTGA GGGAACTCAA TTTGAACTAC AATGAGAACA CTAGACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:CH211-278P9.4 ( XM_009297173.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009295448.1 Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X4, mRNA.

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CloneID	ODa47025
Clone ID Related Accession (Same CDS sequence)	XM_009297173.3 , XM_009297173.2
Accession Version	XM_009297173.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT, LRR and PYD domains-containing protein 12 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTGGAATA CTGCTGTATC GGTGAGGAAG GTTGTGCTGC TCTGGTTTCA 
GGTCTGAAAT CGAACCCGTC ACACCTGAGG GAACTCAATC TGAACAGAAA CGAACCTGGA 
AACTTAGGTG CAAAGATGCT GTCAGATCTC CTGAAGGATC CGCTGTTTGA ACTTGAGAAA 
CTAGAGCTCG ATGACTGCAG TATTGAAAAC AATGGTTTTG CTGATCTAGT TTCAGGCCTG 
AAATCAAACC CATCAAACAT CAGAGAGCTC AATCTGAACT CTAATAAATT CATCTTCTCT 
GGATTAAATC TACTCTGTGA CCTGCTGAGA GATCAGCAGT GTAAACTGCG GAAGCTACAG 
CTGGAGTGCT GCAATATAGA TGAGGAAGGC TGTGCTGCTC TGGCTTTAGC ACTGATATCT 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AATTACAACA ATCCAGGAGG CCTTGGAGTG 
AAGCTTCTGT CTGTTCTTCT GAAGAATCCA CACTGTAAAC TGGAGAAACT GGAGTTGTCT 
CATTGCAGTA TTTCAGAGAA TTACCTTGCT GCTCTAAATT CAGCTCTGAG TTCAAACCCA 
GCACACTTGA GGGAACTCAA TTTGAACTAC AATGAGAACA CTAGACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295448.1
CDS	193..3663
Translation	MDSRKRRSSSPESSCVSIKSDASMDNPPTLRLGESPAKHRRSSSLGSSCVSMKSDASMDNPPTLRLGESPAKHRRSSSPESSCVSMKSDASMDNPPTLRLGESPAKHSDNQKRSQSNKGQKQLDAIFKDLEETNIDEMRKVLRRFKMLLSPDYSVRDEEEEEGQRQINEAFLKITLHALQKMNQTDLASKLQTKLISVSQQQLKSRLKQRFERFTEGVSNRGNTKLLNDIYTEVYITEGGAGNIDDEHEVRQIETSSSRFPEIPIRCCDIFKSLLGQDKAIRTVLTKGVAGIGKTASVQKFILDWAEGKANQDVHFIFPLPFRDLNLIKEKRMSLMELLCCFCPELKPIDYNNFKTVFIYDGLDEWRLPLDFRNNEILWDMNKVASVDALLTNLLQGNLLPSAVHWITTRPAAACQIPAEFIDRVTEVRGFKDLQRAEYFWKRIKDQNLANKIITHIQSSRSLHIMCRIPVFCWISATVLEKIFGEANSADIPKTLTQMFIHLLIVQTNLKTQKYDRECDVNLEKARKSILSLAKLAFEQLEKGNLIFYEEDLKQCGIDVREASVCSGVCTQIFREEFGLQLQKVFSFVHLSVQEFLAALFKFLSFAEENNKSSMTQFMKNEVDRALQSPNGHLDLFLRFLLGLSLDSNQTLLQGLFTPLDTSHSRDETIEYIKSKIRENPSPERSINLFYCLNELNDHSLVQEVQTYLTSTRSHRLSGVKLSPALWSALVFVLLNSTKDLDEFELRQYDFTEECLLRLLPVVKASRKADLKGGNLTDQICSALASALSSSSCCLRELDLSENRLQLSGIRLFSAGLKSPNCKLEILKLSNCSIREEGCASLVSALLSNPSHLRELNLNSNQPGYSTVKLLSDLLENPHCKLEKLQLYNCCIGEEGCAALALALISNPSHLRELNLNNNEPGDSGVKLLSDLLKDPRCKLEKLQLEYCCIGEEGCAALVSGLKSNPSHLRELNLNRNEPGNLGAKMLSDLLKDPLFELEKLELDDCSIENNGFADLVSGLKSNPSNIRELNLNSNKFIFSGLNLLCDLLRDQQCKLRKLQLECCNIDEEGCAALALALISNPSHLRELNLNYNNPGGLGVKLLSVLLKNPHCKLEKLELSHCSISENYLAALNSALSSNPAHLRELNLNYNENTRQ

Target ORF information:

RefSeq Version	XM_009297173.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-278p9.4 (si:ch211-278p9.4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297173.2

ORF Insert Sequence:

ATGGACTCCA GAAAGAGGAG ATCCTCCTCT CCTGAGTCCA GCTGTGTGTC CATAAAGAGT 
GACGCTTCAA TGGACAATCC ACCAACACTC AGATTGGGAG AATCTCCTGC GAAACACAGG 
AGATCCTCCT CTCTTGGATC CAGCTGTGTG TCCATGAAGA GCGACGCCTC AATGGACAAT 
CCACCAACAC TCAGATTGGG AGAATCTCCT GCGAAACACA GGAGATCCTC CTCTCCTGAG 
TCCAGCTGTG TGTCCATGAA GAGCGACGCT TCAATGGACA ATCCACCAAC ACTCAGATTG 
GGAGAATCTC CTGCTAAACA CAGTGACAAC CAAAAGCGAT CACAGTCAAA TAAAGGCCAG 
AAGCAGCTAG ACGCCATTTT CAAGGACCTT GAGGAAACAA ACATAGATGA GATGAGGAAA 
GTGTTGAGAA GATTCAAGAT GCTGCTTAGT CCAGATTACT CTGTGAGAGA TGAGGAGGAG 
GAGGAAGGTC AACGTCAAAT AAACGAGGCA TTTCTGAAGA TCACTCTTCA TGCCCTCCAG 
AAGATGAACC AGACGGACCT CGCTAGCAAA CTACAAACCA AGTTAATCTC CGTGAGTCAA 
CAACAGCTCA AATCCAGACT GAAGCAGAGA TTTGAGAGAT TTACTGAAGG AGTCTCAAAT 
CGAGGCAATA CAAAACTTCT GAATGACATC TACACAGAGG TTTACATCAC TGAGGGAGGG 
GCTGGAAACA TTGACGATGA GCACGAGGTG AGACAGATCG AGACCTCGTC GTCCAGATTT 
CCAGAGATAC CGATTAGATG TTGCGACATC TTCAAATCCT TACTGGGACA GGACAAAGCC 
ATCAGAACGG TTCTGACCAA AGGAGTAGCT GGAATCGGAA AAACCGCTTC TGTGCAGAAG 
TTCATCCTAG ACTGGGCAGA AGGAAAAGCC AATCAGGATG TTCACTTCAT ATTTCCGCTT 
CCTTTCAGAG ACTTGAATTT GATAAAGGAG AAACGGATGA GTCTGATGGA GCTCCTTTGC 
TGTTTTTGCC CAGAACTAAA ACCGATAGAC TATAACAACT TCAAAACAGT CTTCATCTAT 
GATGGTCTGG ATGAGTGGCG CCTTCCTCTG GATTTCCGGA ACAATGAGAT TTTGTGGGAT 
ATGAACAAAG TGGCCTCTGT GGATGCACTG CTTACCAACC TCCTCCAGGG GAACCTGCTT 
CCTTCTGCTG TCCACTGGAT CACCACACGA CCAGCCGCAG CCTGTCAGAT CCCAGCTGAG 
TTTATTGATC GGGTCACCGA GGTACGAGGG TTCAAAGATC TTCAGAGAGC AGAGTATTTC 
TGGAAAAGAA TAAAAGATCA GAATCTTGCC AACAAGATCA TCACACACAT TCAATCATCA 
AGAAGCCTTC ACATCATGTG CCGCATCCCA GTGTTCTGCT GGATCTCCGC CACTGTTCTT 
GAGAAGATCT TTGGTGAAGC GAATAGTGCA GACATTCCCA AAACTCTCAC TCAAATGTTC 
ATACACCTGC TGATCGTTCA GACCAATCTG AAGACCCAGA AATATGACCG AGAATGTGAT 
GTAAATCTTG AGAAGGCAAG AAAGAGCATC TTGTCACTTG CGAAACTGGC ATTTGAGCAA 
CTGGAGAAAG GGAACCTGAT CTTCTATGAA GAGGATTTGA AACAGTGTGG CATTGATGTC 
CGAGAAGCGT CCGTGTGCTC TGGAGTGTGT ACCCAGATCT TTCGGGAAGA GTTTGGGCTG 
CAGCTTCAAA AGGTGTTTAG CTTTGTACAT CTGAGTGTTC AGGAGTTTCT CGCTGCCTTG 
TTTAAGTTTC TTTCCTTTGC TGAAGAAAAC AACAAGTCCT CAATGACACA GTTTATGAAG 
AACGAAGTGG ACAGGGCCTT GCAGAGTCCG AACGGACACC TTGATCTCTT CTTACGTTTT 
CTTCTGGGTC TTTCACTGGA CTCCAATCAG ACTCTCCTAC AGGGATTATT CACTCCGTTG 
GATACCTCTC ACAGCAGAGA CGAGACTATT GAGTATATAA AAAGTAAAAT CAGAGAAAAT 
CCGTCTCCGG AGAGATCCAT CAATCTGTTT TACTGCCTAA ATGAGCTGAA TGATCATTCT 
CTGGTGCAGG AGGTCCAAAC ATATCTAACC AGCACACGCA GCCATCGCCT CTCTGGAGTG 
AAACTGTCTC CGGCCCTGTG GTCAGCACTG GTGTTTGTGT TGCTGAACTC GACAAAGGAC 
CTGGACGAGT TTGAGCTCAG GCAATACGAT TTTACAGAGG AGTGTCTTCT GAGGCTGCTA 
CCGGTGGTCA AAGCTTCCAG AAAAGCAGAT TTGAAAGGTG GTAATCTCAC AGATCAGATC 
TGTTCAGCTC TGGCCTCTGC TCTCAGCTCG AGCTCCTGCT GTCTGCGAGA ACTGGACCTC 
AGTGAAAACA GGCTCCAGCT TTCAGGAATA CGACTTTTCT CTGCAGGACT GAAGAGCCCT 
AACTGTAAGC TGGAGATACT CAAGTTGAGC AACTGCAGTA TTAGAGAGGA AGGCTGTGCT 
TCTCTGGTTT CTGCTCTTCT CTCAAACCCG TCTCATCTGA GAGAACTCAA CCTGAACTCC 
AATCAACCAG GATATTCAAC AGTGAAGCTT TTGTCTGATC TTCTGGAGAA TCCACACTGT 
AAACTGGAGA AACTACAGTT GTATAACTGC TGTATTGGTG AGGAAGGCTG TGCTGCTCTG 
GCTTTAGCTC TGATATCAAA CCCGTCTCAT CTGAGAGAAC TCAATCTGAA CAATAATGAA 
CCAGGCGATT CAGGAGTGAA GCTTCTCTCT GATCTGCTGA AGGATCCGCG CTGTAAACTG 
GAGAAACTAC AGCTGGAATA CTGCTGTATC GGTGAGGAAG GTTGTGCTGC TCTGGTTTCA 
GGTCTGAAAT CGAACCCGTC ACACCTGAGG GAACTCAATC TGAACAGAAA CGAACCTGGA 
AACTTAGGTG CAAAGATGCT GTCAGATCTC CTGAAGGATC CGCTGTTTGA ACTTGAGAAA 
CTAGAGCTCG ATGACTGCAG TATTGAAAAC AATGGTTTTG CTGATCTAGT TTCAGGCCTG 
AAATCAAACC CATCAAACAT CAGAGAGCTC AATCTGAACT CTAATAAATT CATCTTCTCT 
GGATTAAATC TACTCTGTGA CCTGCTGAGA GATCAGCAGT GTAAACTGCG GAAGCTACAG 
CTGGAGTGCT GCAATATAGA TGAGGAAGGC TGTGCTGCTC TGGCTTTAGC ACTGATATCT 
AACCCGTCTC ATCTGAGAGA ACTCAATCTG AATTACAACA ATCCAGGAGG CCTTGGAGTG 
AAGCTTCTGT CTGTTCTTCT GAAGAATCCA CACTGTAAAC TGGAGAAACT GGAGTTGTCT 
CATTGCAGTA TTTCAGAGAA TTACCTTGCT GCTCTAAATT CAGCTCTGAG TTCAAACCCA 
GCACACTTGA GGGAACTCAA TTTGAACTAC AATGAGAACA CTAGACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SI:CH211-278P9.4 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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