MDC1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDC1
Entrez Gene ID	569805
Full Name	mediator of DNA damage checkpoint 1
Synonyms	im:7163673,si:ch73-309n4.5
General protein information	Preferred Names mediator of DNA damage checkpoint 1 Names mediator of DNA damage checkpoint protein 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001365560.1	NP_001352489.1	mediator of DNA damage checkpoint 1
XM_017351464.1	XP_017206953.1	mediator of DNA damage checkpoint protein 1
XM_693212.8	XP_698304.5	mediator of DNA damage checkpoint protein 1
XM_017351464.2	XP_017206953.1	mediator of DNA damage checkpoint protein 1
NM_001365561.1	NP_001352490.1	mediator of DNA damage checkpoint 1
XM_693212.7	XP_698304.5	mediator of DNA damage checkpoint protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45855	NM_001365560.1 Latest version!	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45855	NM_001365561.1 Latest version!	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45855	XM_693212.8 Latest version!	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45855	XM_017351464.2 Latest version!	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa45855	XM_017351464.1	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa45855	XM_693212.7	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDC1 ( NM_001365560.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001352489.1 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 1, mRNA.

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CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	NM_001365560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	mediator of DNA damage checkpoint 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP016064.5, CU984574.3 and CR848747.9. On Sep 1, 2018 this sequence version replaced XM_017351464.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDQH01008296.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA4476737, SAMEA4476741 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352489.1
CDS	43..5787
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	NM_001365560.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365560.1

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( NM_001365561.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001352490.1 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 2, mRNA.

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CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	NM_001365561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	mediator of DNA damage checkpoint 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP016064.5, CU984574.3 and CR848747.9. On Sep 1, 2018 this sequence version replaced XM_693212.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GFIL01013369.1, GDQH01008296.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352490.1
CDS	46..5790
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	NM_001365561.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365561.1

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017351464.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206953.1 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	XM_017351464.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of DNA damage checkpoint protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206953.1
CDS	25..5769
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	XM_017351464.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351464.1

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_693212.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_698304.5 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

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CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	XM_693212.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_693212.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698304.5
CDS	66..5810
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	XM_693212.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693212.8

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017351464.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017206953.1 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

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CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	XM_017351464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017206953.1
CDS	27..5771
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	XM_017351464.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351464.2

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_693212.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_698304.5 Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

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CloneID	ODa45855
Clone ID Related Accession (Same CDS sequence)	NM_001365560.1 , XM_017351464.1 , XM_693212.8 , XM_017351464.2 , NM_001365561.1 , XM_693212.7
Accession Version	XM_693212.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of DNA damage checkpoint protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_693212.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_698304.5
CDS	78..5822
Translation	MMDATQQIEDPFCEEEEEEEDKGDPKREQLATLKVFKNDHIPEAEFPLYVGENILGRDPAACSVLLPARSVSSLHAVISISVFHSSKRSDNGEAVEALIWDKGSLNGTRKGRLKLTPQVRYALTEGESVVLADVPCQYIGLDISKRDTLKTPEKGVCVKEKKESPVLPNSDSKLRKSVQNGLNDKEKTALPPVPLWSPEEEEPSICSPQSAHKQPEITLVPESDSDGESATDERKDFVSDSASSSNLSSSASSSFLTPAKKVIPESEDESSFTPSSASVNRFRRSESNCEPSSNSSKHGPLALNLDSGTDFEEEEMKTSKTEPLATPEVAARIEPVSLAEFHMDSDTDVEEEELETDNAGPEAQVAKKSVTSAGLRVDSDTDVEEEENNKTEPEAQIEQSPKVGPVSSGDLHLESDTDVEEEEEDKSKPDPVAQTETPRLAPVSSADLHIDSDTDVEEEESDASKAKPEAHNAVTLDKPAPSPGLHIDSDTDVEEDEEEKSKTKQKAEIIKSVASTDLHMDSDTDVEEEEPEASNAKPGDLDKSESSVGQCHMDSDTDVEDDNPVQVPQPIGKGIDVKPATSTPGTELNMDSDTDVDEENEDKEKTKDYLSNGGTDDEDPFKPVARKTVKVFKSQEASGGGDEGEQSEGSILKKQDQHSQAKASLEEPTQAFGHLEDDWDFIPTQAYGAASTSAVLKPKRLDLEATQAYGIETDREEEQEEPLSHIQPYTNLSTAETQLIPVAKPGDYAEEEDEETPNDSHLSTADTVIIASTPRREEQTQAFSLFTAQTQLVWEGEGKEVDQSEPTQLIEAESNDEMQEGENEEMIKQGMKTHGTGAVQNSKNSSSYLIIPETQPMCEDEEAIAADLSGDEFQKPSIRKQDKKYDTSHYDKNIPSSHVTLAETQPMCDDDELPGQDDVLSSVQTEKHVSSHIAIAETQPMFEEEEVQDDDLKTEASRRHTDKDEPVQYTEEASTSGFSISKTQPVCGENVRQELRSKTSSRRSCRGLSKKGKDVPLPDEAVSSPAITETQPICMEEKAPDDNLKGEVSSGSTRQQAGEFKSIELAETEPISDEQQAPGEDMPDAARPRRIDEDNSIEFQKPSISHFTIDETQPVVEENEGHEKMFSNKTASRRSCRGKAKKEEEVSQTAETAENQPIPEELIERAELNSEVKSRRSRRTRLIKEEKVPQPSDVVIGSVSTISKTLSVYEDETERDEDLSVIRSKRSCRGRQKDEGEPPKAAELNAGLTVVEAHHMFEENAQEEPSSRTSRRQGKDKAKEESVIPASSKVQVKSRKVQDGKRKRGKELSEDEVESEEEKIIRKGTRRTGLKQKCSEKEEEESVEERNVQEEKEKMKKEQVETERLQKEENERNEQEHEESERAETERQKLEQEQKEQLKREEDEKRQENERIQREKDEEREEKERLERKRNEQEEQRQKEEEGIQRDKEQKENEILQAEKEQLEKERQENEARAQKERDEGSLKKGQKNKQLDKNKSELEPPLLPPTRRSRRCSSSSLNSDQSATSQQEPTSQRGRGRGRGRGRGKQTTGEQPISARQSSRRGPAFEVADTEQDSSLTTRSRSRSSSRSSERSANSVQSSNQGTRGRGRARKSVNLPQPEEVSQTKPSERGRGRRGRTSGVENVDVGMSNELKQDAKMVEDNTAVSQTNSRGRKRTAITSTTAEEALSPTPKTPRRSLASQAHKVLFTGLTDESGERVVSRLGGTLAKGVNDMTHLVTDKARRTVKFLCAVARGVPIVTPDWLKKCGKAGHFISADDYILKDIEQEKKFDFCLEKSLQTAQSQSLLKGYEIHVTPSVMPEPSQMKEIITCCGARFLPKMPSAHKEHVVVVSCEQDRVLCAKAVGMSLPVVSTEFLLTGILQQRVDLQAYSLTSSLDTSNQPPAPKKAAARGRRK

Target ORF information:

RefSeq Version	XM_693212.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator of DNA damage checkpoint 1 (mdc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_693212.7

ORF Insert Sequence:

ATGATGGATG CCACTCAGCA GATTGAGGAT CCCTTCTGTG AAGAAGAAGA GGAAGAGGAG 
GATAAAGGAG ACCCAAAAAG AGAACAGTTA GCAACATTAA AGGTGTTTAA AAATGACCAC 
ATACCAGAGG CAGAATTTCC TCTCTATGTC GGAGAAAATA TCTTGGGTCG GGATCCTGCT 
GCCTGTTCTG TTCTGCTTCC GGCCCGATCT GTGTCCAGTC TGCATGCTGT CATCTCCATC 
TCTGTATTTC ACTCTAGTAA ACGTTCTGAT AATGGTGAGG CTGTGGAAGC ACTTATATGG 
GACAAGGGAA GTTTAAATGG TACCAGAAAG GGCAGATTAA AGCTGACGCC TCAGGTACGA 
TACGCTTTGA CGGAAGGAGA GAGTGTGGTG CTCGCTGACG TGCCTTGTCA GTACATTGGC 
TTGGACATCT CCAAGAGAGA TACATTGAAA ACTCCAGAAA AAGGAGTGTG TGTAAAGGAG 
AAAAAAGAAA GTCCAGTGCT ACCGAATAGT GACTCAAAGT TGAGGAAAAG TGTGCAGAAT 
GGGTTAAATG ACAAAGAGAA AACGGCTTTG CCTCCTGTGC CTTTATGGAG CCCTGAAGAA 
GAAGAACCCA GCATATGTTC ACCACAGTCA GCTCACAAAC AGCCTGAAAT CACCCTGGTG 
CCTGAATCAG ACTCCGATGG AGAGAGCGCT ACAGATGAGC GGAAAGATTT TGTTTCAGAC 
TCTGCGTCAT CTTCTAATCT GTCTAGTTCC GCAAGTTCAT CCTTCCTGAC GCCTGCAAAA 
AAAGTAATTC CAGAGAGTGA GGATGAGAGC TCATTCACTC CATCTTCTGC CTCAGTCAAC 
CGATTCAGAC GGAGTGAAAG TAACTGTGAA CCCAGTTCAA ACTCATCTAA GCATGGCCCC 
TTGGCCCTAA ACTTGGACAG TGGCACTGAT TTTGAGGAAG AGGAGATGAA GACAAGCAAG 
ACTGAACCAT TGGCTACACC AGAAGTAGCT GCAAGAATAG AGCCAGTATC TTTGGCAGAA 
TTTCATATGG ACAGTGATAC TGATGTTGAG GAAGAGGAGC TAGAGACAGA TAATGCTGGT 
CCAGAGGCTC AGGTAGCTAA GAAGTCTGTA ACCTCAGCTG GTCTACGTGT AGATAGTGAT 
ACTGATGTTG AAGAGGAAGA GAATAACAAA ACCGAACCAG AGGCTCAGAT TGAACAATCT 
CCAAAGGTGG GTCCAGTATC TTCAGGAGAT CTTCATTTGG AAAGTGATAC TGATGTTGAA 
GAAGAGGAGG AAGACAAAAG CAAACCTGAT CCAGTAGCTC AGACTGAAAC TCCAAGGTTG 
GCACCAGTCT CTTCAGCAGA TCTTCATATA GACAGTGATA CTGATGTTGA AGAAGAGGAA 
TCGGATGCAA GTAAGGCAAA ACCAGAGGCT CACAACGCAG TAACTCTTGA TAAACCTGCA 
CCTTCTCCTG GTCTTCATAT AGACAGTGAC ACTGATGTAG AAGAAGATGA GGAAGAGAAA 
AGCAAGACTA AACAAAAGGC TGAAATAATT AAATCAGTAG CTTCAACGGA TCTTCATATG 
GACAGCGATA CTGATGTTGA GGAAGAAGAG CCAGAAGCAA GTAATGCTAA GCCAGGGGAT 
CTTGATAAAT CTGAATCTTC AGTTGGACAG TGTCATATGG ACAGTGATAC TGATGTTGAA 
GATGATAATC CTGTGCAGGT TCCGCAACCC ATTGGAAAGG GAATTGATGT CAAACCTGCT 
ACGTCAACTC CAGGGACAGA GCTTAACATG GATAGTGACA CAGATGTGGA TGAAGAGAAT 
GAGGATAAGG AAAAGACCAA AGATTATCTG TCAAACGGTG GAACAGATGA TGAAGATCCT 
TTTAAACCTG TAGCAAGAAA AACTGTGAAG GTTTTCAAGA GTCAGGAGGC CTCTGGTGGA 
GGTGATGAAG GAGAGCAGTC TGAAGGGAGC ATCCTTAAAA AACAAGATCA ACATTCCCAA 
GCAAAGGCTA GTCTTGAGGA ACCAACACAA GCTTTTGGCC ACCTTGAAGA TGACTGGGAC 
TTTATACCCA CACAAGCATA TGGAGCTGCC AGTACTAGTG CTGTTCTAAA GCCAAAGCGG 
CTTGATCTTG AGGCTACACA GGCATATGGA ATAGAGACAG ACAGAGAGGA GGAACAGGAG 
GAGCCACTGA GCCATATCCA ACCTTACACT AACCTCTCTA CTGCTGAAAC TCAACTCATC 
CCTGTTGCAA AGCCTGGTGA TTATGCTGAG GAGGAGGATG AAGAGACACC AAATGACTCT 
CACCTCTCCA CAGCAGACAC AGTAATTATT GCCAGCACCC CGAGAAGAGA GGAGCAGACT 
CAGGCCTTTT CTCTTTTCAC AGCTCAAACG CAACTCGTCT GGGAGGGAGA AGGCAAAGAA 
GTTGATCAAA GTGAGCCCAC CCAGCTGATA GAAGCTGAAT CAAATGATGA AATGCAGGAG 
GGTGAAAATG AGGAAATGAT TAAACAAGGG ATGAAAACCC ATGGTACCGG AGCAGTTCAA 
AATAGTAAAA ACTCCAGCTC CTATCTTATA ATTCCTGAAA CTCAGCCTAT GTGTGAGGAT 
GAAGAAGCAA TAGCTGCAGA TCTGAGTGGG GATGAATTTC AGAAACCAAG TATTAGAAAA 
CAGGATAAGA AATATGATAC TTCACATTAT GATAAAAACA TCCCCAGCTC CCATGTCACT 
CTAGCAGAAA CACAACCAAT GTGTGATGAT GATGAATTAC CAGGTCAGGA TGATGTATTG 
AGCAGTGTTC AAACAGAAAA ACATGTCAGT TCCCATATTG CCATTGCTGA AACACAACCT 
ATGTTTGAGG AAGAAGAGGT GCAAGATGAT GACCTGAAAA CTGAGGCCAG CAGAAGACAT 
ACTGACAAAG ATGAACCTGT GCAATATACT GAAGAAGCTT CGACCTCTGG TTTTTCCATT 
TCCAAAACAC AACCTGTTTG CGGAGAAAAT GTGAGACAGG AGCTCAGAAG CAAGACATCT 
AGCAGAAGAT CATGCAGGGG TCTATCAAAA AAAGGTAAGG ATGTCCCACT GCCTGATGAA 
GCTGTGTCCA GTCCTGCTAT CACAGAAACG CAACCGATCT GCATGGAAGA AAAAGCACCA 
GATGACAATC TTAAGGGTGA AGTCAGCTCT GGGAGTACAA GGCAACAAGC AGGTGAATTC 
AAGTCTATAG AGCTTGCTGA AACAGAGCCT ATAAGTGATG AACAGCAGGC ACCAGGAGAA 
GATATGCCTG ATGCGGCTAG ACCCAGGCGG ATAGATGAAG ATAACTCCAT AGAGTTTCAA 
AAGCCCTCTA TCTCCCATTT CACCATAGAT GAAACGCAGC CTGTGGTTGA GGAAAATGAG 
GGACATGAGA AAATGTTCAG CAACAAGACA GCAAGCAGAA GGTCATGCAG AGGAAAGGCG 
AAGAAAGAAG AGGAGGTTTC ACAAACTGCT GAGACTGCTG AAAACCAACC AATTCCTGAA 
GAACTTATTG AAAGAGCTGA GCTGAATAGT GAAGTCAAAT CCAGGAGATC CCGCAGGACA 
AGACTTATTA AGGAAGAGAA GGTTCCACAA CCTTCTGATG TGGTTATCGG TTCTGTTTCC 
ACCATATCAA AAACGCTGTC TGTTTATGAG GATGAGACTG AAAGAGATGA AGATCTGAGT 
GTGATTAGAT CCAAAAGATC TTGCAGAGGA CGACAAAAAG ATGAAGGTGA ACCTCCAAAG 
GCTGCTGAAC TAAACGCTGG TCTCACGGTG GTTGAAGCAC ACCATATGTT TGAGGAAAAT 
GCTCAAGAAG AACCTAGTAG CAGAACTAGC AGGAGACAAG GGAAAGATAA GGCAAAGGAG 
GAGAGTGTAA TACCTGCATC TAGTAAAGTC CAAGTTAAAA GTAGAAAAGT GCAGGATGGA 
AAAAGAAAGA GGGGGAAAGA ATTGTCTGAG GATGAGGTAG AAAGTGAAGA GGAAAAAATC 
ATAAGGAAAG GAACCAGAAG AACTGGTTTG AAACAAAAGT GTAGTGAAAA GGAAGAAGAG 
GAAAGCGTGG AGGAGAGGAA TGTCCAGGAA GAGAAAGAAA AAATGAAGAA AGAACAGGTG 
GAGACAGAGA GACTACAGAA GGAAGAAAAT GAGAGAAATG AGCAAGAACA TGAAGAGAGT 
GAGAGAGCTG AAACTGAAAG ACAAAAGCTA GAACAAGAGC AGAAAGAACA ATTGAAAAGG 
GAAGAAGATG AAAAAAGACA AGAGAATGAA AGAATACAGA GGGAAAAAGA TGAAGAAAGA 
GAAGAGAAGG AAAGGTTGGA GAGGAAGAGA AACGAGCAAG AAGAACAAAG ACAAAAAGAG 
GAGGAAGGAA TTCAAAGAGA TAAGGAACAA AAAGAAAATG AAATATTACA GGCAGAGAAG 
GAACAATTAG AAAAGGAGCG GCAAGAAAAT GAGGCAAGAG CACAAAAAGA AAGAGATGAG 
GGGAGCCTTA AGAAAGGACA AAAGAATAAG CAGTTGGACA AGAACAAATC TGAACTAGAA 
CCTCCTCTAC TTCCTCCAAC CAGGCGATCA CGCCGCTGTT CAAGCTCCTC TCTGAATTCT 
GATCAGTCTG CAACCTCACA ACAGGAGCCA ACCAGCCAGA GGGGTAGAGG GCGGGGTAGA 
GGACGGGGCC GAGGGAAACA GACAACTGGC GAGCAGCCCA TCAGTGCTAG ACAGTCTAGC 
CGTAGAGGTC CAGCTTTTGA AGTCGCAGAC ACAGAACAAG ACTCCAGTTT AACAACCCGA 
TCAAGATCTC GCTCCAGTTC TAGAAGTTCT GAGAGATCTG CTAATAGTGT CCAGTCCTCA 
AATCAAGGAA CAAGAGGACG AGGAAGAGCC AGGAAGAGTG TGAATCTACC ACAACCTGAG 
GAAGTTTCCC AGACTAAACC TTCTGAAAGA GGCAGGGGGA GAAGAGGCAG GACTTCTGGT 
GTGGAAAATG TAGACGTCGG AATGAGCAAT GAGCTTAAAC AAGATGCGAA GATGGTTGAA 
GACAATACTG CTGTTTCCCA AACCAACAGC AGAGGTCGCA AGAGAACAGC CATCACAAGC 
ACTACTGCAG AAGAGGCTCT TTCTCCCACA CCCAAGACTC CACGGCGCTC GCTGGCCAGT 
CAGGCACACA AGGTGTTGTT CACTGGACTG ACAGATGAGA GTGGAGAAAG AGTGGTCTCT 
CGGTTGGGTG GAACTCTGGC AAAAGGGGTA AACGATATGA CTCACTTGGT GACTGACAAG 
GCAAGACGCA CTGTTAAATT TTTGTGCGCT GTTGCCAGAG GGGTACCAAT CGTGACTCCT 
GACTGGCTAA AGAAGTGTGG AAAAGCTGGC CATTTCATTT CTGCTGACGA TTATATATTG 
AAAGACATTG AGCAAGAGAA GAAGTTTGAT TTCTGCTTAG AGAAGTCATT GCAAACTGCA 
CAAAGTCAAT CTCTTTTAAA GGGTTATGAG ATTCATGTAA CGCCATCTGT GATGCCAGAG 
CCATCTCAAA TGAAAGAAAT CATAACCTGC TGCGGAGCAC GTTTCCTTCC CAAAATGCCC 
TCTGCTCACA AGGAGCATGT TGTTGTGGTG TCATGTGAGC AGGACAGAGT ACTTTGTGCG 
AAAGCAGTGG GTATGTCATT GCCCGTGGTC AGCACAGAGT TCCTATTGAC TGGCATTCTG 
CAGCAGAGAG TTGACCTGCA GGCCTATTCT CTCACTTCCT CCCTCGACAC GTCCAATCAG 
CCTCCAGCCC CCAAAAAAGC TGCTGCCAGG GGCCGTAGGA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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MDC1 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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