myhz1.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	myhz1.2
Entrez Gene ID	799300
Full Name	myosin, heavy polypeptide 1.2, skeletal muscle
Synonyms	myhc3,ns:zf-e523,si:ch211-158m24.8
General protein information	Preferred Names myosin, heavy polypeptide 1.2, skeletal muscle Names myosin, heavy polypeptide 1.2, skeletal muscle fast skeletal myosin heavy chain 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301554.3	XP_009299829.1	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
NM_001161446.1	NP_001154918.1	myosin, heavy polypeptide 1.2, skeletal muscle
XM_009301554.2	XP_009299829.1	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
XM_005171924.3	XP_005171981.1	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
XM_021476335.1	XP_021332010.1	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
XM_005171924.4	XP_005171981.1	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

Ferulic Acid Promotes Hypertrophic Growth of Fast Skeletal Muscle in Zebrafish Model.
Wen Y, Ushio H
Nutrients9(10)(2017 Sep)

MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos.
Lu C, Wu J, Xiong S, Zhang X, Zhang J, Mei J
Gene62549-54(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following myhz1.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myhz1.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37062	NM_001161446.1 Latest version!	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10537	XM_021476335.1 Latest version!	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10537	XM_005171924.4 Latest version!	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10537	XM_009301554.3 Latest version!	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa10537	XM_009301554.2	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10537	XM_005171924.3	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myhz1.2 ( NM_001161446.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001154918.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), mRNA.

Price & Availability↑

CloneID	ODa37062
Clone ID Related Accession (Same CDS sequence)	NM_001161446.1
Accession Version	NM_001161446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX510649.12. On Jun 2, 2009 this sequence version replaced XM_001336203.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA4476735, SAMEA4476755 [ECO:0000348] ##Evidence-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGCTAAGAA TGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTCC GTGAGCAGTT TGAGGAAGAG CAGGAGGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CCAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAGCTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAACGC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG GAACTTTGAC AAGGTCCTGG CAGAATGGAA GCAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGTCCAAG AAGGCAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCTC TGGAGGAGGC TGAAGGCACT CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAAAGT GAGATCGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAATTA CTGATTCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AGATGGAGAT TCAGCTGAGC CATGCCAATC GCCAGGCTGC TGAGGCTCAG 
AAACAGCTCA GAAATGTTCA GGCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCC 
GTGAGAGGAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAATACTCTG 
ATGCAGTCTG AGATTGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GGCTGCTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACCAA GAAGAAGCTT GAGGCTGACC TTGTTCAGAT CCAGAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCAGAGGACA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GGTCACAGTT AAGGACCTGC AGCATCGTCT GGATGAGGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GTGAAGTTGA GGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AAAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAAAAC 
GTCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CAAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTGCTG AGTCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACAG TGGAAAGGGA AAAGAGGCAG CTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001154918.1
CDS	89..5896
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	NM_001161446.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161446.1

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGCTAAGAA TGCACTGGCC CATGCTGTAC AATCAGCCCG TCATGACTGC 
GACCTGCTCC GTGAGCAGTT TGAGGAAGAG CAGGAGGCAA AGGCTGAGCT GCAGCGGGGA 
ATGTCAAAGG CCAACAGTGA GGTTGCTCAG TGGAGAACCA AATATGAAAC TGATGCCATC 
CAGCGCACAG AAGAACTTGA AGAATCCAAG AAGAAGCTGG CTCAGCGTCT GCAAGAGGCA 
GAGGAACAAA TTGAGGCAGT GAACTCCAAA TGTGCATCTC TGGAGAAGAC CAAACAGAGA 
CTCCAGGGTG AAGTGGAGGA CCTCATGATT GATGTGGAGA GAGCCAACGC TTTGGCTGCC 
AACCTTGACA AAAAGCAGAG GAACTTTGAC AAGGTCCTGG CAGAATGGAA GCAGAAATAT 
GAGGAAGGTC AGGCAGAGCT GGAAGGTGCC CAGAAAGAAG CTCGTTCACT CAGCACTGAG 
CTGTTCAAGA TGAAGAACTC CTATGAGGAG ACTCTGGATC AGCTGGAGAC CCTCAAGAGA 
GAGAACAAGA ATCTGCAGCA GGAGATTTCA GATCTGACAG AGCAGATAGG TGAGACTGGT 
AAGAGCATCC ATGAGCTGGA AAAGTCCAAG AAGGCAGTGG AGACTGAGAA GGCAGAGATT 
CAGACCGCTC TGGAGGAGGC TGAAGGCACT CTGGAGCATG AGGAGTCCAA GATTCTTCGT 
GTCCAGCTTG AGCTAAACCA GGTCAAAAGT GAGATCGACA GGAAGCTTGC AGAGAAGGAT 
GAGGAGATAG AGCAGATCAA GAGGAACAGT CAGAGAATTA CTGATTCCAT GCAGAGCACT 
CTGGACTCTG AAGTCAGAAG CAGGAATGAT GCTCTCAGAA TCAAGAAGAA GATGGAGGGA 
GACCTTAATG AGATGGAGAT TCAGCTGAGC CATGCCAATC GCCAGGCTGC TGAGGCTCAG 
AAACAGCTCA GAAATGTTCA GGCACAACTC AAGGATGCCC AGCTGCACCT TGATGATGCC 
GTGAGAGGAC AGGAAGACAT GAAGGAGCAG GTGGCCATGG TGGAGCGCAG AAATACTCTG 
ATGCAGTCTG AGATTGAAGA GCTGAGAGCT GCTCTGGAGC AGACAGAGAG AGGACGCAAA 
GTGGCTGAAC AAGAGCTGGT GGATGCCAGT GAGCGTGTTG GGCTGCTGCA CTCTCAGAAC 
ACAAGTCTTC TGAACACCAA GAAGAAGCTT GAGGCTGACC TTGTTCAGAT CCAGAGTGAA 
GTTGAAGACA CTGTACAGGA AGCCAGAAAT GCAGAGGACA AGGCCAAGAA GGCCATCACT 
GATGCTGCAA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGTGC TCACCTTGAG 
AGGATGAAGA AGAATCTGGA GGTCACAGTT AAGGACCTGC AGCATCGTCT GGATGAGGCT 
GAGAATCTGG CCATGAAGGG AGGAAAGAAA CAACTCCAGA AACTGGAGTC CAGAGTTCGT 
GAGCTTGAGA GTGAAGTTGA GGCAGAGCAG AGACGTGGAG CTGATGCTGT TAAAGGCGTC 
CGCAAATATG AAAGGAGAGT CAAGGAGCTC ACCTACCAGA CTGAGGAGGA CAAGAAAAAC 
GTCAACAGGC TGCAGGATCT GGTTGATAAG CTTCAGCTGA AGGTCAAGGC TTACAAGAGA 
CAGTCTGAAG AAGCTGAGGA ACAAGCCAAC TCTCACCTGT CAAAGTTGAG GAAGGTGCAG 
CATGAGCTGG AGGAGGCTGA GGAGCGCGCT GACATTGCTG AGTCCCAGGT CAACAAGCTC 
AGAGCCAAGA GCCGTGACAG TGGAAAGGGA AAAGAGGCAG CTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz1.2 ( XM_009301554.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299829.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X3, mRNA.

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CloneID	ODa10537
Clone ID Related Accession (Same CDS sequence)	XM_009301554.3 , XM_009301554.2 , XM_005171924.3 , XM_021476335.1 , XM_005171924.4
Accession Version	XM_009301554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299829.1
CDS	79..5892
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	XM_009301554.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301554.3

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz1.2 ( XM_009301554.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299829.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.

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CloneID	ODa10537
Clone ID Related Accession (Same CDS sequence)	XM_009301554.3 , XM_009301554.2 , XM_005171924.3 , XM_021476335.1 , XM_005171924.4
Accession Version	XM_009301554.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299829.1
CDS	99..5912
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	XM_009301554.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301554.2

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz1.2 ( XM_005171924.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171981.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.

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CloneID	ODa10537
Clone ID Related Accession (Same CDS sequence)	XM_009301554.3 , XM_009301554.2 , XM_005171924.3 , XM_021476335.1 , XM_005171924.4
Accession Version	XM_005171924.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171981.1
CDS	102..5915
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	XM_005171924.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171924.3

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz1.2 ( XM_021476335.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332010.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.

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CloneID	ODa10537
Clone ID Related Accession (Same CDS sequence)	XM_009301554.3 , XM_009301554.2 , XM_005171924.3 , XM_021476335.1 , XM_005171924.4
Accession Version	XM_021476335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332010.1
CDS	77..5890
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	XM_021476335.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476335.1

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myhz1.2 ( XM_005171924.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171981.1 Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.

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CloneID	ODa10537
Clone ID Related Accession (Same CDS sequence)	XM_009301554.3 , XM_009301554.2 , XM_005171924.3 , XM_021476335.1 , XM_005171924.4
Accession Version	XM_005171924.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin, heavy polypeptide 1.2, skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171981.1
CDS	124..5937
Translation	MSTDAEMAVYGKAAIYLRKPEKERIEAQNKPFDAKTACYVVDDKELYVKGTIKSRDGGKVTVITLDTKEERVAKEDDVHPMNPPKFDKIEDMAMMTHLNEPSVLYNLKERYAAWMIYTYSGLFCATVNPYKWLPVYDAEVVAAYRGKKRMEAPPHIFSVSDNAYQFMLTDRENQSVLITGESGAGKTVNTKRVIQYFATVAVQGPEKKKEQAAGKMQGSLEDQIIAANPLLEAYGNAKTVRNDNSSRFGKFIRIHFGTSGKLASADIETYLLEKSRVTFQLPDERGYHIFYQMMTNHKPELIEMTLITTNPYDFPMCSQGQITVASIDDKEELVATDTAIDILGFNNEEKMGIYKFTGAVLHHGNMKFKQKQREEQAEPDGTEEADKIGYLLGLNSADMLKALCYPRVKVGNEFVTKGQTVPQVYNSVSALSKSIYERMFLWMVVRINQMLDTKQQRNFFIGVLDIAGFEIFDFNSMEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIVWEFIDFGMDLAACIELIEKPLGIFSILEEECMFPKATDTSFKNKLYDQHLGKCNAFQKPKPAKGKAEAHFSLVHYAGTVDYNISGWLDKNKDPLNESVVQLYQKSSVKLLATLYPPVVEETGGGKKGGKKKGGSMQTVSSQFRENLGKLMTNLRSTHPHFVRCLIPNESKTPGLMENFLVIHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASVIPEGQFIDNKKASEKLLGSIDVNHDEYRFGHTKVFFKAGLLGTLEEMRDEKLASLVTMTQALCRAYLMRREFVKMTERRDAIYTIQYNVRSFMNVKHWPWMKVYYKIKPLLKSAETEKELATMKEDFVKCKEDLAKAEAKKKELEEKMVALLQEKNDLQLAVASESENLSDAEERCEGLIKSKIQLEAKLKETTERLEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAAQDESIGKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKSKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDLENDKQQSEEKLKKKDFETSQLLSKIEDEQSLGAQLQKKIKELQARIEELEEEIEAERAARAKVEKQRADLSRELEEISERLEEAGGATAAQIEMNKKREAEFQKLRRDLEESTLQHEATAAALRKKQADSVAELGEQIDNLQRVKQKLEKEKSEYKMEIDDLSSNMEAVAKAKANLEKMCRTLEDQLSEIKSKNDENLRQLNDLSAQRARLQTENGEFGRQLEEKEALVSQLTRGKQAFTQQIEELKRQIEEEVKAKNALAHAVQSARHDCDLLREQFEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEESKKKLAQRLQEAEEQIEAVNSKCASLEKTKQRLQGEVEDLMIDVERANALAANLDKKQRNFDKVLAEWKQKYEEGQAELEGAQKEARSLSTELFKMKNSYEETLDQLETLKRENKNLQQEISDLTEQIGETGKSIHELEKSKKAVETEKAEIQTALEEAEGTLEHEESKILRVQLELNQVKSEIDRKLAEKDEEIEQIKRNSQRITDSMQSTLDSEVRSRNDALRIKKKMEGDLNEMEIQLSHANRQAAEAQKQLRNVQAQLKDAQLHLDDAVRGQEDMKEQVAMVERRNTLMQSEIEELRAALEQTERGRKVAEQELVDASERVGLLHSQNTSLLNTKKKLEADLVQIQSEVEDTVQEARNAEDKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLESRVRELESEVEAEQRRGADAVKGVRKYERRVKELTYQTEEDKKNVNRLQDLVDKLQLKVKAYKRQSEEAEEQANSHLSKLRKVQHELEEAEERADIAESQVNKLRAKSRDSGKGKEAAE

Target ORF information:

RefSeq Version	XM_005171924.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin, heavy polypeptide 1.2, skeletal muscle (myhz1.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171924.4

ORF Insert Sequence:

ATGAGTACGG ACGCGGAGAT GGCCGTTTAT GGCAAGGCTG CCATTTACCT CCGTAAGCCT 
GAGAAGGAGA GAATTGAAGC TCAGAACAAA CCTTTTGATG CCAAAACCGC TTGTTACGTG 
GTTGATGACA AAGAGCTGTA CGTCAAGGGA ACAATCAAGA GCAGAGACGG TGGCAAAGTC 
ACCGTTATTA CACTTGACAC TAAGGAGGAG AGAGTTGCTA AGGAGGATGA CGTCCATCCA 
ATGAATCCTC CCAAGTTTGA CAAGATTGAG GACATGGCCA TGATGACCCA TCTCAATGAA 
CCCTCTGTGC TGTATAACCT CAAAGAGCGT TATGCAGCAT GGATGATCTA CACCTACTCT 
GGGCTGTTCT GCGCCACTGT GAACCCCTAC AAGTGGCTCC CAGTGTATGA CGCAGAAGTG 
GTTGCTGCCT ACAGAGGCAA GAAGCGTATG GAGGCTCCAC CCCACATCTT CTCTGTCTCT 
GACAACGCCT ATCAGTTCAT GTTGACTGAC AGAGAGAACC AGTCTGTCCT GATTACTGGA 
GAATCTGGTG CTGGAAAGAC TGTGAACACC AAACGTGTCA TTCAGTACTT TGCCACCGTT 
GCAGTTCAGG GCCCTGAAAA GAAGAAAGAG CAAGCTGCTG GCAAAATGCA GGGCTCTCTT 
GAGGATCAGA TCATTGCTGC CAACCCTCTG CTTGAGGCTT ATGGTAATGC CAAGACTGTG 
AGAAATGACA ACTCCTCTCG TTTTGGTAAA TTCATCAGAA TTCATTTCGG CACGTCTGGA 
AAACTGGCCA GTGCTGACAT TGAGACATAT CTGCTGGAGA AATCTAGAGT GACATTCCAG 
CTTCCAGATG AGAGAGGCTA CCACATCTTC TACCAGATGA TGACCAACCA CAAGCCTGAG 
CTGATTGAAA TGACGCTCAT CACCACCAAC CCCTATGACT TCCCCATGTG CAGTCAGGGT 
CAGATCACAG TGGCAAGCAT TGATGATAAA GAGGAGCTGG TTGCTACTGA TACTGCTATT 
GACATTCTGG GCTTCAATAA TGAAGAGAAA ATGGGCATCT ACAAGTTCAC TGGAGCTGTG 
CTTCATCATG GTAACATGAA GTTCAAGCAG AAGCAGCGTG AGGAGCAGGC AGAGCCTGAT 
GGCACAGAGG AGGCTGACAA AATTGGTTAC CTTCTGGGTT TGAACTCTGC TGATATGCTG 
AAGGCTTTGT GCTACCCCAG AGTCAAGGTC GGAAATGAGT TTGTGACCAA AGGACAGACT 
GTACCACAGG TGTACAACTC TGTTAGCGCC TTGTCCAAAT CTATCTATGA GAGGATGTTC 
TTGTGGATGG TCGTTCGTAT CAACCAGATG TTGGACACAA AACAACAAAG AAATTTCTTC 
ATTGGTGTGC TGGATATTGC TGGCTTTGAG ATCTTTGATT TCAACAGCAT GGAGCAGCTG 
TGCATCAACT TCACTAATGA GAAACTGCAA CAGTTTTTCA ACCACCACAT GTTTGTGCTG 
GAACAAGAGG AGTACAAGAA GGAGGGCATT GTTTGGGAGT TCATTGACTT CGGCATGGAC 
TTGGCCGCTT GCATTGAGCT CATTGAGAAG CCCTTAGGTA TCTTCTCCAT CCTTGAAGAG 
GAGTGCATGT TCCCCAAGGC TACAGACACT TCCTTCAAGA ACAAGCTGTA TGATCAGCAT 
CTTGGCAAGT GCAATGCTTT CCAGAAACCA AAGCCTGCCA AAGGCAAGGC TGAGGCCCAC 
TTCTCCCTGG TCCACTATGC TGGAACTGTG GACTACAACA TTTCTGGCTG GCTGGACAAG 
AACAAGGATC CACTGAACGA GTCTGTTGTG CAGCTTTACC AGAAGTCTTC AGTCAAACTG 
CTGGCTACTC TCTACCCACC TGTTGTTGAG GAGACTGGCG GTGGAAAGAA GGGAGGAAAG 
AAGAAGGGTG GTTCCATGCA GACTGTGTCT TCCCAGTTCA GGGAGAACTT GGGCAAGCTC 
ATGACCAACT TGAGGAGCAC TCACCCTCAC TTTGTGCGTT GTCTGATTCC CAATGAGTCC 
AAGACTCCAG GTCTTATGGA GAACTTCCTG GTTATCCACC AGCTGAGGTG TAACGGTGTA 
CTGGAGGGTA TCAGAATCTG CAGAAAGGGT TTCCCCAGCA GAATCCTCTA TGCTGACTTC 
AAGCAGAGAT ACAAGGTGTT GAATGCCAGT GTTATCCCAG AGGGACAGTT CATTGATAAC 
AAGAAGGCCA GCGAGAAACT CCTGGGATCT ATTGATGTTA ATCATGATGA GTACAGATTT 
GGACACACAA AGGTGTTCTT CAAAGCTGGT CTTCTGGGTA CTCTTGAGGA GATGCGTGAT 
GAGAAACTGG CTAGTCTGGT CACAATGACT CAGGCTCTCT GCCGTGCTTA CCTGATGAGG 
AGAGAGTTTG TAAAGATGAC GGAGAGGAGA GATGCAATTT ACACCATCCA GTACAATGTT 
CGCTCATTCA TGAATGTCAA ACACTGGCCA TGGATGAAGG TTTACTACAA GATTAAGCCT 
CTGCTGAAGA GTGCTGAGAC TGAGAAAGAG CTGGCAACCA TGAAAGAGGA CTTTGTCAAA 
TGCAAAGAAG ATCTTGCCAA GGCTGAAGCT AAAAAGAAGG AGCTGGAAGA GAAGATGGTG 
GCACTGCTGC AAGAGAAAAA TGATCTGCAG CTGGCAGTGG CTTCTGAATC AGAGAATCTC 
TCAGATGCTG AGGAAAGATG TGAGGGTCTG ATCAAGAGCA AAATCCAGCT TGAGGCTAAA 
CTCAAAGAGA CAACTGAGAG ACTGGAGGAT GAGGAAGAAA TCAATGCTGA ACTGACAGCC 
AAGAAGAGGA AACTGGAGGA CGAGTGCTCT GAGCTGAAGA AAGACATTGA TGACCTGGAG 
CTCACCTTGG CTAAAGTGGA AAAGGAGAAA CATGCCACAG AGAATAAGGT CAAGAACTTG 
ACAGAGGAAA TGGCAGCTCA AGATGAGAGC ATTGGTAAGC TTACAAAGGA GAAGAAAGCC 
CTCCAAGAGG CACATCAGCA GACTCTGGAT GATCTCCAGG CTGAGGAGGA CAAAGTCAAC 
ACCCTGACCA AATCTAAGAC AAAGCTTGAG CAGCAAGTTG ATGATCTGGA AGGTTCCCTT 
GAGCAAGAGA AGAAGCTCCG TATGGACCTG GAGAGAGCCA AGAGAAAGCT TGAAGGAGAC 
TTGAAATTGG CACAAGAGTC CATCATGGAC CTGGAGAATG ACAAGCAGCA GTCTGAGGAA 
AAGCTGAAGA AGAAAGACTT TGAAACAAGC CAGCTCCTCA GCAAGATTGA AGATGAACAA 
TCTTTGGGTG CTCAGCTCCA GAAGAAGATC AAGGAGCTTC AGGCTCGCAT TGAGGAACTG 
GAGGAAGAGA TTGAGGCTGA GCGTGCTGCT CGTGCCAAGG TTGAGAAGCA GAGAGCCGAT 
CTCTCCAGGG AACTTGAGGA GATCAGTGAG AGGCTTGAGG AGGCTGGAGG AGCCACTGCT 
GCTCAAATCG AGATGAATAA GAAGCGTGAA GCTGAATTCC AGAAGCTGCG TCGTGATCTT 
GAAGAGTCCA CCCTTCAGCA TGAAGCTACT GCTGCTGCCC TGCGCAAGAA GCAGGCAGAC 
AGTGTGGCCG AGCTGGGAGA GCAAATCGAC AACCTCCAGC GTGTCAAGCA GAAGCTTGAG 
AAAGAGAAGA GTGAATACAA AATGGAGATT GATGATCTCT CCAGCAACAT GGAGGCTGTT 
GCCAAAGCAA AGGCCAATCT AGAGAAGATG TGCCGCACAC TTGAGGACCA ACTTAGTGAA 
ATTAAGTCTA AGAATGATGA GAACCTTCGC CAGTTGAATG ATCTCAGTGC TCAAAGAGCA 
AGACTTCAAA CTGAAAATGG TGAGTTTGGC CGTCAGCTTG AGGAGAAGGA AGCTCTGGTT 
TCTCAGCTCA CACGAGGAAA ACAGGCTTTC ACTCAGCAAA TTGAGGAGCT TAAGAGGCAG 
ATTGAAGAGG AGGTTAAGGC TAAGAATGCA CTGGCCCATG CTGTACAATC AGCCCGTCAT 
GACTGCGACC TGCTCCGTGA GCAGTTTGAG GAAGAGCAGG AGGCAAAGGC TGAGCTGCAG 
CGGGGAATGT CAAAGGCCAA CAGTGAGGTT GCTCAGTGGA GAACCAAATA TGAAACTGAT 
GCCATCCAGC GCACAGAAGA ACTTGAAGAA TCCAAGAAGA AGCTGGCTCA GCGTCTGCAA 
GAGGCAGAGG AACAAATTGA GGCAGTGAAC TCCAAATGTG CATCTCTGGA GAAGACCAAA 
CAGAGACTCC AGGGTGAAGT GGAGGACCTC ATGATTGATG TGGAGAGAGC CAACGCTTTG 
GCTGCCAACC TTGACAAAAA GCAGAGGAAC TTTGACAAGG TCCTGGCAGA ATGGAAGCAG 
AAATATGAGG AAGGTCAGGC AGAGCTGGAA GGTGCCCAGA AAGAAGCTCG TTCACTCAGC 
ACTGAGCTGT TCAAGATGAA GAACTCCTAT GAGGAGACTC TGGATCAGCT GGAGACCCTC 
AAGAGAGAGA ACAAGAATCT GCAGCAGGAG ATTTCAGATC TGACAGAGCA GATAGGTGAG 
ACTGGTAAGA GCATCCATGA GCTGGAAAAG TCCAAGAAGG CAGTGGAGAC TGAGAAGGCA 
GAGATTCAGA CCGCTCTGGA GGAGGCTGAA GGCACTCTGG AGCATGAGGA GTCCAAGATT 
CTTCGTGTCC AGCTTGAGCT AAACCAGGTC AAAAGTGAGA TCGACAGGAA GCTTGCAGAG 
AAGGATGAGG AGATAGAGCA GATCAAGAGG AACAGTCAGA GAATTACTGA TTCCATGCAG 
AGCACTCTGG ACTCTGAAGT CAGAAGCAGG AATGATGCTC TCAGAATCAA GAAGAAGATG 
GAGGGAGACC TTAATGAGAT GGAGATTCAG CTGAGCCATG CCAATCGCCA GGCTGCTGAG 
GCTCAGAAAC AGCTCAGAAA TGTTCAGGCA CAACTCAAGG ATGCCCAGCT GCACCTTGAT 
GATGCCGTGA GAGGACAGGA AGACATGAAG GAGCAGGTGG CCATGGTGGA GCGCAGAAAT 
ACTCTGATGC AGTCTGAGAT TGAAGAGCTG AGAGCTGCTC TGGAGCAGAC AGAGAGAGGA 
CGCAAAGTGG CTGAACAAGA GCTGGTGGAT GCCAGTGAGC GTGTTGGGCT GCTGCACTCT 
CAGAACACAA GTCTTCTGAA CACCAAGAAG AAGCTTGAGG CTGACCTTGT TCAGATCCAG 
AGTGAAGTTG AAGACACTGT ACAGGAAGCC AGAAATGCAG AGGACAAGGC CAAGAAGGCC 
ATCACTGATG CTGCAATGAT GGCAGAGGAG CTGAAGAAGG AGCAGGACAC CAGTGCTCAC 
CTTGAGAGGA TGAAGAAGAA TCTGGAGGTC ACAGTTAAGG ACCTGCAGCA TCGTCTGGAT 
GAGGCTGAGA ATCTGGCCAT GAAGGGAGGA AAGAAACAAC TCCAGAAACT GGAGTCCAGA 
GTTCGTGAGC TTGAGAGTGA AGTTGAGGCA GAGCAGAGAC GTGGAGCTGA TGCTGTTAAA 
GGCGTCCGCA AATATGAAAG GAGAGTCAAG GAGCTCACCT ACCAGACTGA GGAGGACAAG 
AAAAACGTCA ACAGGCTGCA GGATCTGGTT GATAAGCTTC AGCTGAAGGT CAAGGCTTAC 
AAGAGACAGT CTGAAGAAGC TGAGGAACAA GCCAACTCTC ACCTGTCAAA GTTGAGGAAG 
GTGCAGCATG AGCTGGAGGA GGCTGAGGAG CGCGCTGACA TTGCTGAGTC CCAGGTCAAC 
AAGCTCAGAG CCAAGAGCCG TGACAGTGGA AAGGGAAAAG AGGCAGCTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ferulic Acid Promotes Hypertrophic Growth of Fast Skeletal Muscle in Zebrafish Model.
Nutrients9(10)(2017 Sep)
Wen Y,Ushio H

MicroRNA-203a regulates fast muscle differentiation by targeting dmrt2a in zebrafish embryos.
Gene62549-54(2017 Aug)
Lu C,Wu J,Xiong S,Zhang X,Zhang J,Mei J

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myhz1.2 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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